prot_P-fluviatile_contig6.12533.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A6H5JBC7_9PHAE (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBC7_9PHAE)

HSP 1 Score: 1347 bits (3486), Expect = 0.000e+0
Identity = 711/857 (82.96%), Postives = 759/857 (88.56%), Query Frame = 0

Query:    1 MSGFPPPSSSMPPVNIGQSATIAASNADAVGAGSGS----------------------NGVSSTISGDGVVFEGGAVFGSMMVGTVPAMGGGLGQSAPPSPELGPTPSANRSGPSGPVHHSRASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTFDPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGLYEDSDEEDGGLDDMIVRQAEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGGDKVSPGFKTEYG--NLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSRGNEYTHKPMEPEPGAW 833
            MSGFPPPS+S+PPVNIGQSA+ A         G GS                        +SS +SG      GG ++G+MMVG++PAMGGGLG+SAPPSPELGP PSANRSG SGPVHHSRA+LMSA+RIV+KAGTSIVSNSRGYPCLSR+GA+VEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKG+GRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYE DGMFSDNDGLAALVAEQMNAKML+LLTDVEGVYNKHPDEEGAKIFHTFDP+RHELMIG+KSAGGRGGMGAKIRAASRAVSGGVPSVVIASG+NPYSIERIVCGERVGTMFCANPGLYEDSD+E+   DDMIVRQAE ARVGGR LCAL SEERAGILRKVAEALL                         LLNRLKLTEDKIKVLAEGI+SLADGEEPL KCNSRLEVQ+GL+LKQIT+PIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAE+SNACLHRIIV+AVAEGS GRV RDCIGLVT+RAAVGELLKLDHLIDLVIPRGSG+LVNHIKASTKIPVMGHSEGICHVYIDKAADPTKA E+AVDAKVNYPSACNAAETILIH  LL +GAGTC+QVVRALHS GVTVLGGPRAV+LGVVGGDK+SPGFKTEYG  +LTV++EIVDSVEAA+DHIQ +GSSHTETIVTEDQ+VA+ F+E VDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGV+GLLTTKWVLRSHGE AHTV + + Y  KPM+ E GAW
Sbjct:    1 MSGFPPPSNSLPPVNIGQSASTAG--------GGGSVXXXXXXXXXXXXXXXXTTVPVADMSSIVSG------GGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGKSGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEED-ADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW 842          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: D7G5M6_ECTSI (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5M6_ECTSI)

HSP 1 Score: 1046 bits (2706), Expect = 0.000e+0
Identity = 547/662 (82.63%), Postives = 579/662 (87.46%), Query Frame = 0

Query:  172 MGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTFDPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGLYEDSDEEDGGLDDMIVRQAEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGGDKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSRGNEYTHKPMEPEPGAW 833
            MGAVLDGHAEVPKG+GRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYE DGMFSDNDGLAALVAEQMNAKML+LLTDVEGVYNKHPDEEGAKIFHTFDP+RHELMIG+KSAGGRGGMGAKIRAASRAVSGGVPSVVIASG+NPYSIERIVCGERVGTMFCANPGLYEDSD+E+   DDMIVRQAE ARVGGR LCAL SEERAGILRKVAEALL                         LLNRLKLT+DKIKVLAEGI+SLADGEEPL KCNSRLEVQ+GL+LKQIT+PIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAE+SNACLHRIIV+AVAEGS GRV RDCIGLVT+RAAVGELLKLDHLIDLVIPRGSG+LVNHIK                                    VNYPSACNAAETILIH  LL +GAGTCSQVVRALHSAGVTVLGGPRAV+LGVVGGDK+SPGFKTEYG+LTV++EIVDSVEAA+DHIQR+GSSHTETIVTEDQ+VA+ F+E VDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGV+GLLTTKWVLRSHGE AHTV + + Y+ KPM+ E GAW
Sbjct:    1 MGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEED-ADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTKDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIK------------------------------------VNYPSACNAAETILIHKALLEEGAGTCSQVVRALHSAGVTVLGGPRAVDLGVVGGDKISPGFKTEYGDLTVSLEIVDSVEAALDHIQRFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGETAHTVGKKDPYSLKPMDLEAGAW 625          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A835ZBW4_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBW4_9STRA)

HSP 1 Score: 879 bits (2270), Expect = 2.100e-310
Identity = 472/747 (63.19%), Postives = 562/747 (75.23%), Query Frame = 0

Query:   99 SRASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGR-------RNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTFDPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGLY--EDSDEEDGGLDDMIVRQAEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGGDKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVS-----RGNEYTHKPMEPEPG 831
            SR  ++ A RIVIKAGTS+VS+ RGYP L+R+GA+VEQCAWL+KMGKEV+LVSSGAVGVGRQLLRKQQLL++S+G VLDGH     G+G         +Y+SACAAAGQLGLMSLY+TLF+QCD ATSQ+L+T  DF TPER R+LRYT +TML+LGV+PI+NENDAVS N+GY  DG FSDNDGLAA+VA+QM A++LVLLTDV+GVY+K P   GA+I HT+ P   E+ IG KS GGRGGM AKI AA RAV  GV SVVIA+G NP+SIE++ CGE+VGT+FC N       D  EED  L + +   AE AR GGR LC L +EER   L  VA+AL                          LL RL+LT  KI+ L +GI+SLAD  EP+GKC S LEV +GL+LKQ+T PIGVLL+VFESRPDSLPQIASLA+RSGNGLLLKGGKEAE+SNACLH II +AV E +GGRV R+ IGLV  R AV ELLKLD LIDLVIPRGSG LVN IK++TKIPV+GH+EG+CH++ID AAD  KA  +AVDAK+NYP+ACNA ET+L+H D +    G   +V  AL SAG  VLGGPRA+  G+    + +P FKTEYG  ++++E+VD   AA+DHI R+GS HTE IVTED   AQ F+E VDSACVFHNASTRFSDG+RFGLGAEVG+STGRIHARGPVGV+GLLTTKWVLRS    AHTV+      G  YTHK ++P  G
Sbjct:   38 SRQKVLHAHRIVIKAGTSVVSDERGYPSLTRLGAIVEQCAWLRKMGKEVLLVSSGAVGVGRQLLRKQQLLNSSLGQVLDGH----DGNGAALLQAKSNSYNSACAAAGQLGLMSLYDTLFSQCDTATSQMLLTADDFGTPERCRNLRYTMNTMLQLGVIPIVNENDAVSANQGYTKDGTFSDNDGLAAVVAQQMGAQLLVLLTDVDGVYDKAPTLPGARIIHTYLPAT-EVQIGDKSVGGRGGMSAKISAAMRAVEAGVASVVIANGCNPFSIEQVCCGEKVGTLFCQNAAAMAARDLPEEDEALTEKVRVAAEAARAGGRALCQLSTEERTQALHAVAKALGARQAEVLAANARDMQEAERVKLAGPLLKRLQLTPAKIQTLVDGINSLADQPEPIGKCMSHLEVSEGLVLKQVTTPIGVLLVVFESRPDSLPQIASLALRSGNGLLLKGGKEAEHSNACLHGIIADAVFESTGGRVCREVIGLVVGRQAVAELLKLDALIDLVIPRGSGELVNRIKSTTKIPVLGHAEGVCHMFIDTAADEAKAIALAVDAKINYPAACNALETLLVHQDCV--ATGLAKKVADALVSAGGKVLGGPRAIAAGLA--TEPTPSFKTEYGENSLSLEVVDDAGAAIDHIHRFGSGHTEAIVTEDAATAQSFLERVDSACVFHNASTRFSDGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRS--SDAHTVAGMGGESGRAYTHKALDPVDG 773          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: W7U194_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U194_9STRA)

HSP 1 Score: 729 bits (1883), Expect = 6.800e-252
Identity = 428/769 (55.66%), Postives = 538/769 (69.96%), Query Frame = 0

Query:  100 RASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTF----DPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCA-------------------------NPGLYEDSDEEDGGLDDMIVRQAEGARVGG--------RMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGGDKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSRGNE-----YTHKPM 826
            R  L  A R+VIKAGTS+VS   GYP L R+  +VEQ   L K G++V+LV+SGAVG+G+QLL KQQ +  S+  V+ GH  + K +G   YDSACAAAGQLGLMSLYETLF+   +ATSQ+LVT +DF   +RR H++Y  +++L+LG+VPI+NENDAVSGNEGY    +FSDND LA+LVA++  A +L+LLTDV GVY+K P +E AK    +     P      IG+KSA GRGGM AKI AA+ A  GGV  VVIASG     I +I  GE  GT+FC                           PG    S   D  L+D++  +AEG   G         R L +L   ER+ IL  VA+ALL    XXXXXXXXXXXXXXXXX    LLNRL LT++K+  LA+GI +LA+ EEP+G+  SR+EV  GL L Q T PIGVLLIVFESRPDSLPQIA+LA+RSGNGLLLKGGKEA +SN  LHR+I +A+   S GRV  + IGLVTTR+ V  LL+LD +IDLVIPRGS ALV  IK+ST+IPV+GH+EG+CH+Y+DK AD  KAA + VDAK +YP+ACNA ET+L+H   + DGA   S+++RAL SAGVTVLGGP AV+ G+V     +   + EYG+LT+T+E+V  +E A+ HI ++GSSHT+ I+TED   A++F+ +VDSACVFHNAS+RF+DGYRFGLGAEVG+STGRIHARGPVGV+GLLTTKW+LRS G  +HTV+   E     YTH+ +
Sbjct:    6 RLRLRDAQRVVIKAGTSVVSTPEGYPSLVRLAHIVEQICLLIKEGRDVLLVTSGAVGIGQQLLAKQQRMSLSVRDVVSGHPPIKKPNG---YDSACAAAGQLGLMSLYETLFSTRQIATSQILVTGYDFTCSDRREHVQYCVNSLLQLGIVPIINENDAVSGNEGYTESTVFSDNDSLASLVAQETQADLLILLTDVCGVYDKPPSDEDAKQLGLYLRDCSP-----GIGEKSAQGRGGMQAKIDAATAAAKGGVEHVVIASGHEAEVIGKIARGENTGTLFCRAALFPPEEDMSASTGSLVGLNGNGDGPGTVCSSGPPDELLEDILALEAEGGIRGQALATKEAQRALASLTGNERSQILLAVADALLANTAXXXXXXXXXXXXXXXXXLAEPLLNRLGLTKEKLMTLADGIRALANMEEPVGRLVSRMEVAAGLELTQTTVPIGVLLIVFESRPDSLPQIAALALRSGNGLLLKGGKEALHSNQVLHRVITDAMTTASEGRVPAEAIGLVTTRSEVSALLELDDVIDLVIPRGSSALVRSIKSSTRIPVLGHAEGVCHIYVDKGADLRKAARIVVDAKTDYPAACNAVETLLLHRSFVADGAIGASKLLRALMSAGVTVLGGPEAVKEGLVSPGHAAKDLRQEYGDLTITVEVVADMEEAIAHIHKFGSSHTDAIITEDPVCAEEFLRSVDSACVFHNASSRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLLRSSG--SHTVADFAEGGDKVYTHQKL 764          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A7S2WAW7_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WAW7_9STRA)

HSP 1 Score: 696 bits (1797), Expect = 4.080e-239
Identity = 385/739 (52.10%), Postives = 497/739 (67.25%), Query Frame = 0

Query:   99 SRASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTF-DPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGLYEDSDEE----DG--GLDDMIVRQAEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGGDKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSRGNE----YTHKPM 826
            SR  L +A RIVIKAGTS+V+N  G+  L+R+  +VEQ + L   G EV+L+SSGAVG GRQ+LRKQ  L  +    L    +   G     YDSACA+AGQLGLMSLY+TLF QCD+  SQ LVT +DF    R++HL    S +++ G+VPI+NENDAVSGN+GY  + +FSDND LA++VA  MNA +L+LLTDV G++NKHP E GAKI +T+         IG KS  GRGGMGAK+ +A R +  GV ++VIASG +  SI  +  G  VGT+F   PG   D  +     DG   L+ M V     AR   R L AL +EER  +++ +A+AL                          L+ RLKLTE K++ LA GI ++++ +EP+G    R E+ DGLIL ++T PIGVLLI+FESRPDSLPQIA+LAIRSGNGLLLKGG+EAE+SNA LHRIIV AV   + G+V++D + LVT+R  V  LL LD  IDLV+PRGS ALV HIK  T+IPV+GH++GICHV++D++AD  KA ++A+D+K +YP+ACNAAETILIH        GT   ++  L  AGVT+ GG RAV LG++     +    TEYG+LT  +E+V  ++ A+DHI  +GSSHTE IVTED E A+ F+  VD+ACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGV+GLLTTK++L S          G+     YTH+ +
Sbjct:   23 SRLDLRNARRIVIKAGTSVVTNPDGHFSLTRLSGIVEQISELIHQGFEVVLISSGAVGCGRQVLRKQAFLSGNFKQQLRTSEDRSTG-----YDSACASAGQLGLMSLYDTLFGQCDICASQFLVTSYDFTDDSRKQHLVECMSRIIEHGMVPIVNENDAVSGNKGYTTENVFSDNDALASIVAANMNADLLILLTDVPGLFNKHPSEPGAKIINTYYSGDASNFTIGAKSTQGRGGMGAKVDSAVRGLDRGVKAIVIASGYHQRSIFDVCLGYPVGTLFL--PGSSPDPKDALCSADGPPSLNSMAVE----ARKAARALQALSTEERVRVMKAIAQALRDNSDEILEANAKDLALAVTNDVAPPLVQRLKLTEQKLETLAAGIEAISEADEPIGHVEGRTELADGLILSKVTVPIGVLLIIFESRPDSLPQIAALAIRSGNGLLLKGGREAEHSNATLHRIIVTAVQTATAGKVSQDLVSLVTSREQVSSLLALDDCIDLVVPRGSKALVEHIKQHTRIPVLGHADGICHVFVDESADLEKAVDIAIDSKTDYPAACNAAETILIHEAAAETMGGT---ILGGLRRAGVTIKGGARAVALGMIPHSDAAESMSTEYGDLTCLVEVVSGIDEAIDHIHSHGSSHTECIVTEDAEQAKVFLARVDAACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKYILESRDGDVVNAFAGDSPSRHYTHRKL 747          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A1W0A8G2_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A8G2_9STRA)

HSP 1 Score: 686 bits (1770), Expect = 1.880e-235
Identity = 383/733 (52.25%), Postives = 510/733 (69.58%), Query Frame = 0

Query:  100 RASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTFDPHRH-ELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGLYEDSDEEDGGLDDMIVRQAEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGGDKVSPGFKTEYGNLTVTIEIVDS-VEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSR----GNEYTHKPM 826
            R    +A RIVIK GTS+VS S G   L RV +L EQ + LK+ GKEV+LV+SG++GVGR+ L+KQ LL +S+   + G++ + +   ++    A AAAGQ+GLMSLYETLF+  DVA SQ+LVTE DF+T E R ++R T   +L+L V+PI+NENDAVS     E + +F+DND LAALVA ++ A +L+LLTDV+G+YNK P E  AKI   F P +  + +IG+KS+ GRGGMG+KI++A  A+  GV +VVIASG     ++ I+ G  +GT+F  +P L     E     +DM +     AR G R L  L S++RA ILRK+A +L+                         L  RLKLT+ K++ LA+GI S+AD  EP+G+  S++EV  GL+L+Q+T PIGVLLIVFESRPDSLPQIA+LA+RSGNGLLLKGGKEA +SNACLH++I +A+   + GRV R  IGLVTTR  V ELLKLDH+IDL IPRGS +LV +IK +T+IPV+GH+EG+CH+YI   A+  KA  + VDAK +YP+ACNA ET+L+  D +   +G    ++ ALH AGV +L GP A  LGV+   + +P    EYG + +T+E++   VE A+ HI  + S HTE+IVTED+ VA++F++ +DSACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGV+GLLTTKW LRS+    HTV+      N+YTHKP+
Sbjct:    9 RQDFQNAKRIVIKMGTSVVS-SNGEAALGRVASLAEQISMLKRQGKEVLLVTSGSIGVGRKKLQKQILLSSSLRMHVQGNSNMSRLLEKKK--GAMAAAGQVGLMSLYETLFSLYDVACSQVLVTESDFKTKEHRANIRDTLLHLLELDVIPIVNENDAVSAGVIREGETVFTDNDSLAALVAGEIKANLLILLTDVDGLYNKPPSEPNAKIISVFVPEQPGQFVIGEKSSVGRGGMGSKIQSAQGAIDNGVNAVVIASGFKYGVLDAIMKGHNIGTLFVQHPELLV---ENKSSAEDMAIS----AREGSRALLQLTSDDRARILRKIAASLI--ENSDTILYANQLDMAEASSVDAALRARLKLTKPKLETLAKGIRSIADQVEPIGQTVSKMEVSSGLVLEQLTCPIGVLLIVFESRPDSLPQIAALALRSGNGLLLKGGKEASHSNACLHKLIGDAIESETQGRVPRGVIGLVTTRDDVSELLKLDHVIDLCIPRGSNSLVTYIKKNTRIPVLGHAEGVCHMYIHSGANMEKAKSLVVDAKTDYPAACNALETLLV--DKVHVDSGAIQSIIDALHDAGVKILVGPTAASLGVLPEAEAAPSLSHEYGAMALTLEVISGGVEKAIAHINAHSSGHTESIVTEDKNVAEQFLDGIDSACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWKLRSNS--VHTVASFTNGHNKYTHKPL 725          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A6A3JB98_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=24 Tax=Peronosporaceae TaxID=4777 RepID=A0A6A3JB98_9STRA)

HSP 1 Score: 679 bits (1751), Expect = 3.680e-232
Identity = 385/738 (52.17%), Postives = 503/738 (68.16%), Query Frame = 0

Query:  100 RASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTFDPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGLYEDSDEEDGGLDDMIVRQ-AEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGG-DKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSRGN--------EYTHKPME 827
            R  L  A+RIV+K GTS+VS + G P L R+ ++VEQ   LK+ GKEV+LV+SGAVG+GR+ L KQ LL  S+   + G+ ++     ++    A AAAGQ+GLMSLYETLF+  DVA SQ+LVT  DF+T + R ++R T   +L+L VVPI+NENDAVS +    V   F+DND LAALV  ++ A +L+LLTDVEG+YNK P + GAKI   F P   +  IG+KS+ GRGGMGAKI AA  A+S GV +VVIASG+    +  I+ G  +GT+F ANPG+           D M V + A  AR G   L AL SEERA IL ++A+ L                          LL RLKL+E+K++ LA+GI S+A+ EEP+G+   R E+  GL+L Q TA IGVLL++FESRPDSLPQIA+LA+RSGNGLLLKGGKEA +SNA LH+IIVNAV E + G+V +D IGLVT+R  + +LL+LD +IDL IPRGSG++V++IK +T+IPV+GH+EG+CH+YI  AAD  KA E+A+DAK +YP+ACNA ET+LI   L+  G     +++ AL  AG+ +   P A +LGV     + +    TEYG+  +TIE+ +  +AA+ HI +Y S HTE IVTED   A+ F + +DSACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGV+GLLTTKW L S  E  HTVS+ +        +YTHK +E
Sbjct:   23 RHDLKHATRIVVKMGTSVVSTN-GEPALGRIASIVEQVCMLKRQGKEVLLVTSGAVGIGRKRLNKQILLSASLRTHVQGNQQMLALEKKKG---AMAAAGQIGLMSLYETLFSLYDVACSQVLVTASDFKTAQNRANMRDTMLNLLELDVVPIVNENDAVSASPDGTV---FTDNDSLAALVGGEIEADLLMLLTDVEGLYNKPPTQPGAKIISVFRPENSDFKIGEKSSVGRGGMGAKIEAAQSAISQGVNAVVIASGMKYGVVGSIMKGSSLGTLFVANPGVESP--------DCMSVEEMATAAREGCHQLQALSSEERANILFRIADELKKNSKTILNANRKDLLAAQKSSIDEGLLVRLKLSEEKLETLADGIRSIAEAEEPIGRMLKRTELASGLVLHQETASIGVLLVIFESRPDSLPQIAALALRSGNGLLLKGGKEALHSNAVLHKIIVNAVEEATNGKVKKDVIGLVTSREEISDLLRLDDVIDLCIPRGSGSMVSYIKKNTRIPVLGHAEGVCHMYIHGAADVKKAIELAIDAKTDYPAACNALETLLIDESLVTSGQD--KEILAALEEAGINLHIAPNAAKLGVATAKSRPTNSLSTEYGSKDLTIEVANGADAAISHINQYSSGHTECIVTEDAAAAELFQQMIDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWKLVS--ESGHTVSQFSGAKNDHPLKYTHKKLE 741          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A7S2BH10_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2BH10_9STRA)

HSP 1 Score: 674 bits (1738), Expect = 2.940e-230
Identity = 377/714 (52.80%), Postives = 485/714 (67.93%), Query Frame = 0

Query:  103 LMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMG------AVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFH-TFDPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERI-VCGERVGTMFCANPGLYEDSDEEDGGLDDMIVRQAEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLAD-GEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGGDKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRS 807
            ++ A R+VIKAGTS+VSN  G   L RV A+VEQ   L K+ KEVIL+SSGAVG GR+ LRKQ LL TS+       + LD  A+  +      Y +A A++GQL LM +YET+F+Q DVA SQ LVTE+DF    RR HL+ +   ++K  +VPI+NENDAVS N+GY  D +FSDND LAALVA+QM A  LVLLTDV+G+Y++ P E  A +    +     E   G KS  GRGGM AK+ AA  A+  GV +VV+ASG +   ++++ V GE  GT+F   P   ++      GL     +QA  AR  GR L  L   ER+G+L+ VA+A+                          LL RLKL+ +K+  +A GI  LAD   +PLGK   R EV +GL L Q++ PIGVLLIVFESRPDSLPQIASLA+ SGNGLLLKGGKEAE+SN  LH+++V+AVA  S G+V R+ +GLVT+R  V +LLKLD LIDLVIPRGS ALV +IK +T+IPV+GH++GICH YID+ AD  KAA +AVD K+NYP+ACNA ET+LIH    +  +G C+ V+R L  AGV++ GGP A+  G++     +     EYG+LT  +E+V ++E A+ HI  +GSSHTE IVTED   A++F++  D+ACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGV+GLLTTKWVLRS
Sbjct:   19 IVDAKRVVIKAGTSVVSNEDGTLSLVRVAAIVEQVYALLKLQKEVILISSGAVGCGRRKLRKQSLLGTSLRKQMHSCSSLDDLADEDESE---FYSAASASSGQLELMKVYETMFSQFDVAASQFLVTEYDFNNSSRRSHLKNSMEEIIKHCMVPIINENDAVSANQGYTDDNIFSDNDALAALVAQQMGADALVLLTDVDGLYDRAPSEPDATVIPIVYRSAGDEFSYGAKSNQGRGGMSAKVGAAMGALEAGVKAVVVASGKDTTVLQKLFVTGEGAGTLFLEQPPSTDEPAVGVSGLTPTSAQQAASARDAGRKLQGLSFSERSGVLKAVAQAIRDNEESILLANSRDMSAAKQNNLAPALLQRLKLSHEKLVTVARGIDQLADLAADPLGKVLGRTEVAEGLQLTQLSVPIGVLLIVFESRPDSLPQIASLALLSGNGLLLKGGKEAEHSNYTLHKVVVDAVAAASDGKVPREIVGLVTSRTQVSDLLKLDRLIDLVIPRGSKALVTNIKKNTRIPVLGHADGICHTYIDQHADDVKAAAIAVDGKINYPAACNATETVLIHQ--AKVDSGGCATVMRELRKAGVSLKGGPNAIAQGLIDMSCAAKSLSVEYGDLTCLVEVVANMEEAIAHIHEHGSSHTECIVTEDTATAEEFIKTTDAACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRS 727          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A0N7L745_PLAHL (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0N7L745_PLAHL)

HSP 1 Score: 670 bits (1728), Expect = 9.460e-229
Identity = 379/738 (51.36%), Postives = 493/738 (66.80%), Query Frame = 0

Query:  100 RASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTFDPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGLYEDSDEEDGGLDDMIVRQ-AEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGVVGG-DKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSRGN--------EYTHKPME 827
            R  L +A+RIV+K GTS+VS + G P L R+ ++VEQ   LK+ GKEV++V+SGAVG+GR+ L KQ LL  S+   + G+ ++           A AAAGQ+GLMSLYETLF+  DVA SQ+LVT  DF+T + R ++R T   +L+L VVPI+NENDAVS +    V   F+DND LAALV  ++ A +L+LLTDVEG+YNK P   GAK+   F P  +   IG+KS+ GRGGMGAKI+AA  A+S GV +VVIASG     +  IV G  +GT+F ANPG            D M   + A  AR G   L  L SEER  IL +VA+ L                          L+ RLKLTE+K+  L +GI S+A+ EEP+G+   R E+  GLIL Q TAPIGVLL++FESRPDSLPQIA+LA+RSGNGLLLKGGKEA +SNA LHRIIVNAV E S G+V +D IGLVT+R  + +LL +D++IDL IPRGSG++V+HIK +T+IPV+GH+EG+CH+YI  AAD  K+ E+A+DAK +YP+ACNA E++L+   ++  G     +++  L  A + +  GP A  LGV     + +    TEYG+  +TIE+V  + AA+ HI +Y S HTE IVTED   A+ F   VDSACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGV+GLLTTKW+L S  E  H VS+ +        +YTH+ ++
Sbjct:   19 RVDLKNATRIVVKMGTSVVSTN-GEPALGRIASIVEQVCMLKRQGKEVLVVTSGAVGIGRKRLNKQILLSASLRTHVQGNQQM---LALEKKQGAMAAAGQIGLMSLYETLFSLYDVACSQVLVTASDFKTAQNRANMRDTMLNLLELDVVPIVNENDAVSASPDGTV---FTDNDSLAALVGGEIGADLLMLLTDVEGLYNKPPTHPGAKVISVFIPENNNFRIGEKSSVGRGGMGAKIKAAQSAISQGVKAVVIASGFRYGVVASIVKGANLGTLFVANPGAESP--------DSMSAEEMAFSARKGSHQLQMLSSEERVHILFRVADDLKRNFSTILAANRQDLLAAQKSSIDEGLIARLKLTEEKLDTLIDGIRSIAESEEPIGRILKRTELASGLILHQETAPIGVLLVIFESRPDSLPQIAALALRSGNGLLLKGGKEARHSNAELHRIIVNAVEEASQGKVKKDVIGLVTSRDEISDLLCMDNVIDLCIPRGSGSMVSHIKKNTRIPVLGHAEGVCHMYIHSAADINKSIELAIDAKTDYPAACNALESLLLDEAVVSSGQD--KKILAKLEKADINLHIGPNAASLGVATAKSRPTDSLSTEYGSKDLTIEVVHGLSAAISHINQYSSGHTECIVTEDAAAAETFQRRVDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWILAS--ESGHIVSQFSGTKCKSSLKYTHRKLD 737          

BLAST of mRNA_P-fluviatile_contig6.12533.1 vs. uniprot
Match: A0A5D6YC09_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YC09_9STRA)

HSP 1 Score: 665 bits (1716), Expect = 1.000e-226
Identity = 384/737 (52.10%), Postives = 495/737 (67.16%), Query Frame = 0

Query:   99 SRASLMSASRIVIKAGTSIVSNSRGYPCLSRVGALVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGSGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEVDGMFSDNDGLAALVAEQMNAKMLVLLTDVEGVYNKHPDEEGAKIFHTFDPHRHELMIGQKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGINPYSIERIVCGERVGTMFCANPGL-YEDSDEEDGGLDDMIVRQAEGARVGGRMLCALKSEERAGILRKVAEALLGXXXXXXXXXXXXXXXXXXXXXXXXLLNRLKLTEDKIKVLAEGISSLADGEEPLGKCNSRLEVQDGLILKQITAPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEYSNACLHRIIVNAVAEGSGGRVARDCIGLVTTRAAVGELLKLDHLIDLVIPRGSGALVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAAEVAVDAKVNYPSACNAAETILIHNDLLRDGAGTCSQVVRALHSAGVTVLGGPRAVELGV-VGGDKVSPGFKTEYGNLTVTIEIVDSVEAAVDHIQRYGSSHTETIVTEDQEVAQKFMEAVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVKGLLTTKWVLRSHGEKAHTVSRGNE-------YTHKPM 826
            SR  L  A RIV+K GTS+VS + G P L R+ ++VEQ   LK+ GK+V+LV+SG+VG+GR+ L KQ LL  S+   + G+  +           A AAAGQ+GLMSLYETLF+  DVA SQ+LVT  DF+T + R ++R T   +L+L V+PI+NENDAVS      V   FSDND LA+LV  +++A +L+LLTDVEG+YNK P + GA+I   F  +     IG KSA GRGGMG+KI AA  A++ GV +VVIASG     I  ++ G   GT+F ANPGL    S       +DM V     AR G R L  L S+ERAGILR++A  L+                         LL RLKLT+ K++ LA+GI+ +A  EEP+GK   R E+  GL+L+Q TAPIGVLL++FESRPDSLPQIA+LA+RSGNGLLLKGGKEA  SNA LHRIIV+AVA  + GRV++  +GLVT+R A+ +LL+LD +IDL IPRGSGA+V+ IK +T+IPV+GH+EG+CH+YI + A    A  +AVDAK +YP+ACNA ET+LI   L+  G  T   V+ AL  AGV +  GP+A +LG+     + +    TEYG   +T+E+V  VEAAV+HI  + S HTE IVTE+   A +F + VDSACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGV+GLLTTKW L S  E+ HTV++ +        YTHK +
Sbjct:   30 SRQDLQHARRIVVKMGTSVVSTN-GEPALGRIASIVEQICMLKRQGKDVLLVTSGSVGIGRKRLNKQILLSASLRTHVQGNQHM---LALEKKTGAMAAAGQIGLMSLYETLFSLYDVACSQVLVTASDFKTAKNRANMRDTMLNLLELDVIPIVNENDAVSAASDGSV---FSDNDSLASLVGGEISADLLLLLTDVEGLYNKPPSQPGARIIPVFHTNNASFEIGDKSAVGRGGMGSKIDAAQGAINRGVKAVVIASGFKYGIIMSVIDGGNHGTLFVANPGLPASGSAAASASAEDMAVA----ARAGCRQLQKLSSDERAGILRRIAHELVAQSDAILKANEADLYAAEVSSLDEGLLARLKLTKAKLQTLADGIAGIARQEEPVGKVLKRTELASGLVLRQETAPIGVLLVIFESRPDSLPQIAALALRSGNGLLLKGGKEAAQSNAILHRIIVDAVAAETRGRVSKSVVGLVTSREAIADLLRLDDVIDLCIPRGSGAMVSSIKKNTRIPVLGHAEGVCHMYIHRDAALATAITLAVDAKTDYPAACNALETLLIDEALIAGGQATA--VLAALEQAGVVLHIGPQAHKLGLGTPRSRTTTALSTEYGTKELTVEVVGGVEAAVEHINAHSSGHTECIVTENAAAAAQFQQDVDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWKLCS--ERGHTVAQFSSAHAQPLAYTHKQL 751