prot_P-fluviatile_contig6.12394.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: Q8QN66_ESV1K (EsV-1-224 n=2 Tax=root TaxID=1 RepID=Q8QN66_ESV1K)

HSP 1 Score: 284 bits (727), Expect = 2.210e-91
Identity = 161/320 (50.31%), Postives = 209/320 (65.31%), Query Frame = 0

Query:   14 WIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYNDLF---HQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREHSRLGV-----VLSHLADVESCLSEGGSEALGVAAVTSAFH 325
            W EK+RP  L+DV GN  VIRAL S+ +    P+++LHGPPG+GKTS+I+++A+SF+G T  S M LELNA D R + T+R++I  FTRCS V +     L K+VILDEAD LT  +Q ALR LI+ +  R RFCLCCN+IS+LS GL SRCTSF FS I   +L  T R VA+KE L ISE+ L+ VVK+  GDAR+ INLLQS+SL     L     Q++Y+ CG+P P++   + E LL +SF+  Y  L   V     SL Q+I  L E +++     +GV     VLS LADVE CLS GGSE + V A+  AFH
Sbjct:    4 WTEKHRPTRLQDVSGNDHVIRALQSFTSMETTPNMILHGPPGSGKTSSIISMAKSFFGRTNISIMTLELNAGDSRSIGTMRDQIHFFTRCSSVTDRSTNAL-KLVILDEADGLTSCAQRALRHLIETTSGRARFCLCCNYISRLSSGLRSRCTSFSFSGIGNVQLAHTLREVARKEDLEISEEGLNAVVKVCAGDARQGINLLQSLSLGDATTLVVRDDQAVYATCGLPSPSQTSEIFEVLLNQSFSVGYTTLTSFVELYQFSLLQMIPPLVESIISCSDKDIGVDTVGEVLSTLADVERCLSNGGSEGIAVGAMVGAFH 322          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: A0A6H5KU33_9PHAE (MEP9 protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KU33_9PHAE)

HSP 1 Score: 277 bits (709), Expect = 1.040e-82
Identity = 156/317 (49.21%), Postives = 211/317 (66.56%), Query Frame = 0

Query:   14 WIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYNDLF---HQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREHSRLGV-----VLSHLADVESCLSEGGSEALGVAAVTS 322
            W EK+RP  L+DV GN  VIRAL ++K+    P+L+LHGPPG+GKTS+IL++A+SF+G T  S+M LELNA D R + T+R++I  FTRCS V++     L K+VILDEAD LT  +Q ALR LI+ +  R RFCLCCN+I++LS GL SRCTSF FS I   +L  T R VA KE L ISE+ L+ VV +  GDAR+ INLLQS+SL     L     Q++Y+ CG+P P++   + + LL +SF+  Y  L  L+     SL Q+I  L E +++    ++GV     VLS LADVE CL++GGSE + V A+ +
Sbjct:    4 WTEKHRPTRLQDVSGNDQVIRALQTFKSMEATPNLILHGPPGSGKTSSILSMAKSFFGGTNISTMTLELNAGDSRSIGTMRDQINFFTRCSSVSDKSTNAL-KLVILDEADGLTSCAQRALRHLIETTCGRARFCLCCNYINRLSSGLRSRCTSFSFSGIGNVQLARTLRKVAHKERLEISEEGLNAVVNVCAGDARQGINLLQSLSLGHATTLTARDDQAVYTTCGLPSPSQTSEIFDVLLNQSFSVGYTTLMSLIDLCQFSLTQMIPRLVESIISCGDEKMGVDRVGEVLSTLADVERCLTDGGSEGIAVGAMAN 319          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: A0A7E4ZQQ8_PANRE (AAA domain-containing protein n=1 Tax=Panagrellus redivivus TaxID=6233 RepID=A0A7E4ZQQ8_PANRE)

HSP 1 Score: 228 bits (580), Expect = 5.650e-69
Identity = 131/328 (39.94%), Postives = 203/328 (61.89%), Query Frame = 0

Query:    1 MSTVAREC-RGSLPWIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVA-EAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYNDLFHQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREHSRLGV--VLSHLADVESCLSEGGSEALGVAAVTSAF 324
            M TV  E  +G+LPW+EKYRP  L D+  + ++I  L  +    +LPHLL +GPPG GKTS ILA AR  Y      SMVLELNASD RG+  VR EI  F +   +  +A+GK   K++ILDEAD++T  +Q ALR +I++  D VRFC+ CN++SK+   L SRCT FRF+ ++K ++L     V   E L+++E   D +  LS GD RR +N+LQS +L+ ++ +   ++YS  G P P +I  +L++LL  +F++A+  L++L   +  +L  +I  + + +++ +     V  ++  LA ++S L+ G SE L + A+ S+F
Sbjct:    1 MVTVPSEAPKGNLPWVEKYRPSTLSDLVSHQEIINTLNKFVDSNQLPHLLFYGPPGTGKTSTILAAARQIYTAKQYRSMVLELNASDDRGIGIVREEIVSFAQSRMLHYDADGKASYKLIILDEADAMTKDAQNALRRVIEKFTDNVRFCIICNYLSKIIPALQSRCTRFRFAPLSKDQVLPRLNHVIAAENLTVTETGKDALFALSGGDMRRVLNVLQSTALA-FDTVNEDNVYSCVGQPRPKDITLILKTLLDGTFDDAFKSLNELRISSGIALTDVIDNVLDKVISMKIKAEAVCGLVDALATIQSRLATGCSEKLQLLALVSSF 327          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: RFC5_DICDI (Probable replication factor C subunit 5 n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=RFC5_DICDI)

HSP 1 Score: 224 bits (570), Expect = 1.940e-67
Identity = 128/318 (40.25%), Postives = 189/318 (59.43%), Query Frame = 0

Query:   11 SLPWIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLS-VYNDLFHQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREHSR--LGVVLSHLADVESCLSEGGSEALGVAAVTSAFH 325
            SLPW+EKYRP+ L+D+  + D+ + +        LPHLL +GPPG GKTS I AIAR  YGD   S MVLELNASD RG+D VR +I+ F    F          K++ILDEADS+T+++Q ALR +I++     RFC+ CN++ K+   L SRCT FRFS +         + + +KE + +    ++ V++L  GD R+ +N+LQSVS+S + N++  ++IY   G P P++I  +++ LL   + EA+  + DL      SL  IIA L + +V  +     L  +LSHL+D+E  LS G SE L + ++   F 
Sbjct:   23 SLPWVEKYRPKNLDDLIAHEDITQTITKLIDNNTLPHLLFYGPPGTGKTSTIQAIARKLYGDN-YSRMVLELNASDDRGIDVVREQIKTFASSMFFFNTT--VPYKLIILDEADSMTNIAQTALRRVIEKYTKTTRFCIVCNYVIKIIPALQSRCTRFRFSPLPTPPTEIRLKEIIEKENVKVDSKAMNAVLELGCGDMRKCLNILQSVSMSSIDNNITEEAIYKCTGYPMPSDIELMVDWLLNSDYEEAFQNISDLKKKKGLSLNDIIATLQKFVVQIDLDNVILCKLLSHLSDIEYNLSIGSSEKLQLGSLVGCFQ 337          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: A0A2T0FIM6_9ASCO (Replication factor C subunit 3 n=1 Tax=Wickerhamiella sorbophila TaxID=45607 RepID=A0A2T0FIM6_9ASCO)

HSP 1 Score: 223 bits (567), Expect = 2.560e-67
Identity = 127/314 (40.45%), Postives = 192/314 (61.15%), Query Frame = 0

Query:   11 SLPWIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYND--LFHQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREHSRL--GVVLSHLADVESCLSEGGSEALGVAAV 320
            +LPWIEKYRP  L DV G+  VI+ +  +    ++PHLL +GPPG GKTS I+A+AR  YG     +MVLELNASD RG+D VRNEI+ F     +  ++G    K+VILDEAD++T+++Q ALR +I++     RFC+  N+  KL+  L+SRCT FRFS +    +    + V   E ++I+   LD +V L+HGD RRA+N+LQ+    V     +  + IY   G P+P ++  +L S+LK+S++ AY  +  L      +LA I+  L + ++  E  +   G +LS +A++E  LS GG+E +  +A+
Sbjct:    4 NLPWIEKYRPASLNDVSGHEGVIKTIRRFVENKQIPHLLFYGPPGTGKTSTIVAVAREIYGPN-YKNMVLELNASDDRGIDVVRNEIKTFASTQQIF-SKG---FKLVILDEADAMTNVAQNALRRIIEKYSAHTRFCIIANYTHKLNPALISRCTRFRFSPLTSDAVRSRCQDVIAAESIAITPTALDALVTLAHGDMRRALNVLQACHARVERGTPIDEKDIYDCVGSPDPRDVQRILTSMLKESWSTAYDVITTLKRDKGLALADILQCLTDEVITLELDQTVRGKILSGMAEIEWRLSSGGTEKIQTSAL 312          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: A0A1A8W655_PLAMA (Replication factor C subunit 3, putative n=6 Tax=Plasmodium (Plasmodium) TaxID=418103 RepID=A0A1A8W655_PLAMA)

HSP 1 Score: 222 bits (566), Expect = 7.090e-67
Identity = 120/315 (38.10%), Postives = 191/315 (60.63%), Query Frame = 0

Query:   13 PWIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYNDLFHQS-IYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREH--SRLGVVLSHLADVESCLSEGGSEALGVAAVTSAF 324
            PW+EKYRP VL D+  +  VI  +  +    ELPHLLLHGPPG GKTS ILA+ +  YGD+  SS VLELNASD RG++ VR++I+ F           KT  K++ILDEAD +T  +Q A+R +++     VRFC+ CN+++K++  + SRCTSF+FS + +  ++     +AK E + ++++ L++++++  GD RR +N LQ VSLS  N    ++ I S   +P P E+  +LE  +K S  E+Y  +  + +    S+  I+  L E ++  +   S + ++L +  ++E   S G SE + ++A+ SAF
Sbjct:   14 PWVEKYRPNVLNDIISHEQVISTIQKFVEKGELPHLLLHGPPGTGKTSTILAVCKELYGDSR-SSFVLELNASDDRGINVVRDQIKTFAESKNHYNTCEKTSLKLIILDEADHMTFPAQNAMRRIMENYAKNVRFCMLCNYVNKITPAIQSRCTSFKFSPLKEEYMINKALDIAKSENVKLTKEGLESLIRVGRGDMRRILNCLQVVSLSHKNMTIDENVILSTLDIPLPNEVKDILEHFMKSSIKESYEFVTKMQYSKGYSIKDIMVCLYESILTYDFPDSAVCLLLKNFGEIEERCSSGASEQITLSALISAF 327          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: C1C1X9_CALCM (Replication factor C subunit 5 n=1 Tax=Caligus clemensi TaxID=344056 RepID=C1C1X9_CALCM)

HSP 1 Score: 221 bits (562), Expect = 2.900e-66
Identity = 133/332 (40.06%), Postives = 195/332 (58.73%), Query Frame = 0

Query:    1 MSTVARECRGS---LPWIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYNDLFHQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELH-DLVFGNHCSLAQIIA---LLAEHLVAREHSRLGVVLSHLADVESCLSEGGSEALGVAAVTSAFH 325
            M T   E +G+   LPW+EKYRP+ L D+  + D++  +  +   + +PH+L +GPPG GKTSAILA +R  +G+T  SS VLELNASD RG+D  R  I  F     +A   G    K++ILDEAD++T+ +Q ALR +I++  D VRFCL CN++SK+   L SRCT FRF+ +A  ++L   + V K+E L +S D    ++ L+ GD RR +N+LQS S++ +  +   +IY+  G P P++I   LESLL     +AY  +H  L      SL  I+    LL   L      ++ +++  LAD E  L  G SE + + AV +AF 
Sbjct:    1 MVTSTMEPKGAALNLPWVEKYRPKDLSDLVSHEDIVGTIRRFVKESRMPHMLFYGPPGTGKTSAILAASREVFGETSNSS-VLELNASDDRGIDVARGRILNFASTKRIAIQAGTASFKLIILDEADAMTNDAQNALRRIIEKFTDNVRFCLICNYLSKIIPALQSRCTRFRFAPLASEQILPRLQAVVKEESLEMSPDGAKALLTLAKGDMRRILNILQSCSMA-FPVINESNIYACTGHPPPSDISLALESLLNDDLQKAYQTIHVQLQTTKGLSLIDILTELHLLVHRLEISNRVKVKLLIK-LADAEHRLLSGTSEKIQLGAVVAAFR 329          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: A0A2R5GU05_9STRA (Replication factor C subunit 5 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GU05_9STRA)

HSP 1 Score: 221 bits (562), Expect = 3.750e-66
Identity = 134/328 (40.85%), Postives = 192/328 (58.54%), Query Frame = 0

Query:    1 MSTVARECRGSLPWIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECF--TRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYNDLFHQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREH--SRLGVVLSHLADVESCLSEGGSEALGVAAVTSAF 324
            M+   +E   +LPWIEKYRP+ L+D+  + +++  +        LPHLL +GPPG GKTS ILA+AR  YG +   SMVLE+NASD RG+DTVRNEI  F  TR  F       T  K+VILDEAD++T  +Q ALR +I++     RFC+ CN+ SK+   L SRCT FRF+ +    ++   + +  KE L +SE  LD V+KL  GD R+ +N+LQS SL+    +   ++Y   G P  A+I A   +L  +S +EA+  + +L      +L  I+  L E L A +   S    +L H+A++E  L+ G SE L + A+  AF
Sbjct:    1 MADKLQEETEALPWIEKYRPQKLDDLVAHDEIVATIRKLVDANRLPHLLFYGPPGTGKTSTILAVAREMYGSS-MRSMVLEMNASDARGIDTVRNEISNFAGTRKLF------STGIKLVILDEADNMTKDAQMALRRVIEKYASNARFCIICNYASKIIPALQSRCTRFRFAPLKTQLVVGRVKEIIAKEELEVSEAGLDAVLKLGEGDMRKVLNILQSSSLANNRKVDEAAVYRCTGKPTAADILACRSALFSESVSEAFQTMRNLQVTKGLALVDILQALHEDLKAVKFAPSTRVQLLKHMAEIEYNLASGTSETLQLGALVGAF 321          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: A0A1X7R1Q3_9SACH (Similar to Saccharomyces cerevisiae YNL290W RFC3 Subunit of heteropentameric Replication factor C (RF-C) n=2 Tax=Kazachstania TaxID=71245 RepID=A0A1X7R1Q3_9SACH)

HSP 1 Score: 220 bits (560), Expect = 4.590e-66
Identity = 125/331 (37.76%), Postives = 201/331 (60.73%), Query Frame = 0

Query:    1 MSTVARECRGSLPWIEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSV----YNDLFHQSIYSYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIA----LLAEHLVAREHSRLGVVLSHLADVESCLSEGGSEALGVAAVTSA 323
            MST A + R +LPW+EKYRP+ L+DV G   ++  +  +    +LPHLL +GPPG GKTS I+A+AR  YG    S+MVLELNASD RG+D VRN+I+ F     +  +      K++ILDEAD++T+ +Q ALR +I++     RFC+  N+  KL+  L+SRCT FRF  +    +      V   E + +S+     ++KLS GD RR +N+LQ+   ++     +++  ++IY  CG P PA++ A+L+S+L+  ++ AY+ L+ +   N  +L  +I     +L E+ +  E +R+ + L+ L D+E  +S+GG++ +  +AV  A
Sbjct:    1 MSTQADKKRENLPWVEKYRPQTLDDVYGQTSIVTTVRKFIEEGKLPHLLFYGPPGTGKTSTIVALARELYGKN-YSNMVLELNASDDRGIDVVRNQIKDFASTRQIFSSG----FKLIILDEADAMTNAAQNALRRIIEKYTKNTRFCILANYAHKLTPALLSRCTRFRFQPLPSDAIERRIANVLVHEHIKLSDQAKIALLKLSKGDMRRVLNVLQAGKATLDDPEKDEITDETIYECCGAPRPADVTAILKSILEDDWSTAYYTLNKIRQTNGLALIDLIEGMIEILEEYELQNETTRIEL-LTRLGDIEYSISKGGNDRIQSSAVIGA 325          

BLAST of mRNA_P-fluviatile_contig6.12394.1 vs. uniprot
Match: A0A1J1H8Q3_PLARL (Replication factor C3, putative n=2 Tax=Plasmodium (Haemamoeba) TaxID=418104 RepID=A0A1J1H8Q3_PLARL)

HSP 1 Score: 220 bits (560), Expect = 4.870e-66
Identity = 121/313 (38.66%), Postives = 190/313 (60.70%), Query Frame = 0

Query:   15 IEKYRPRVLEDVRGNADVIRALLSYKTPAELPHLLLHGPPGAGKTSAILAIARSFYGDTGCSSMVLELNASDYRGVDTVRNEIECFTRCSFVAEAEGKTLAKMVILDEADSLTHLSQCALRSLIDRSGDRVRFCLCCNFISKLSEGLVSRCTSFRFSTIAKSELLCTTRTVAKKEGLSISEDCLDTVVKLSHGDARRAINLLQSVSLSVYNDLFHQSIY-SYCGVPEPAEIGAVLESLLKKSFNEAYHELHDLVFGNHCSLAQIIALLAEHLVAREH--SRLGVVLSHLADVESCLSEGGSEALGVAAVTSAF 324
            +EKYRP VL D+  +  VI  +  +    ELPHLLLHGPPG GKTS ILA+ +  YGD+  SS VLELNASD RG++ +R++I+ F           KT  K++ILDEAD +T  +Q A+R +++     VRFCL CN+++K++  + SRCT+FRFS + K  ++     +AK E ++++++ L++++++ HGD RR +N LQ VSLS  N    ++I  S   +P P EI  +LE   K S  E+Y  +  L +    S+  I+  L + ++  +   S   ++L +L ++E   S G SE + ++A+ S+F
Sbjct:   11 VEKYRPNVLSDIISHEQVISTIRKFVQKGELPHLLLHGPPGTGKTSTILAVCKELYGDSR-SSFVLELNASDDRGINVIRDQIKTFAESKNHYNTCEKTTLKLIILDEADHMTFPAQNAMRRIMENYAKNVRFCLLCNYVNKITPAIQSRCTAFRFSPLKKEYMMNKALDIAKSENVNVTKEGLESLIRVGHGDMRRILNCLQVVSLSHKNLTIDENIILSTLDIPLPNEIKQILEHFTKSSIKESYEFVSKLQYNKGYSIKDIMKCLYDMVITYDFPDSAFCLLLKNLGEIEERCSSGASEQITLSALISSF 322