prot_P-fluviatile_contig52.11545.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: A0A6H5JMF4_9PHAE (Vps8 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JMF4_9PHAE)

HSP 1 Score: 1620 bits (4196), Expect = 0.000e+0
Identity = 1428/2823 (50.58%), Postives = 1560/2823 (55.26%), Query Frame = 0

Query:   63 SRIGTKQELDRWRGDHNGAGRSKEGAVKQRPPLGYRDTAAXXXXXXXXXXXXXXXPSPPSYGRGGGSSVAYSDRVSGDEGSSTTTGADCNGGSASGALARTAFLSRDEGLEDSLLLQQILRESEAEVAASAADRANXXXXXXXXXXXXXXXXXXXXXXXXXXKGESMTKFASTVGGAGGXGGDLYGAADDNLHSLDVDRIIASMELEAEAEEDGDLMAGRGGSDGGMPSWRASAAGLMATMASSSRNRSVTSNGVTEGGGGGRAMASGLRERGASATGGEGRAGGKGVVSLSPPTSNDGAYGVGATDRPDLAGIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRALRKA-----ETAELRLLRGGNTDIVSPLQVKRRMRAPXXXXXXXXXXXXXXXXXXXXXXXXV--VRMENLKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQ----------EIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPSWTDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRPQLSTNNVGAGAPSEHL------------HLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGTRWPALAVVKELGASAQVVAVEWLREQV---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGSA-------------------------PPRSEAAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXXSSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAVRASRGDYVSSTEEVLLEAISIAEHEQ------PYIEEGAAEAXXXXXXXXERQLWPYPADPSFDELGYKVLLYLERCFQGRVLGAAEPLPAAETARARSSLLHLLVRPSLLQPGSSASSPSEPHDRSAGFXXXXXXXXXXXXXXXXXVCRRRRRWREAMYPYLSVLLYVDAPAALDVLSVAMDSPDANFQRE-PGPAFGGGXXXXXXXXXXXXXGG--------PKLFRESWSSSTSSVSLLATGAEGPEVSAGGTSDGGGRGNEGESRRRKGSARSGEALGGGVWGRRLGQKVRAARAVGWTVGSAAMAAGKADG----DGGGVEGIRKWRRAGLEAVAAAAETSCPSRRMIVEAVTSVLAPHLTADAEQLIAPAXXXXXXXXXXXXXXXXXXXXGDGTPDDGGGLSAAAFVHGNRPAAAPATPAAATAGAGESSTKMAMPTAATAAALSSSLLRDRDGQEWWAAARPHLFGFVAKYLELSLLSVGPRLTNAVLVSAATGTCGGSGGGPVEGGXXXXXXXXXXXXXXXXLGSAGDAQTRLVTLLERVPLAFVDRERLLPLAERSRFFRAAVILLKAAVTLGEQRGYSVSQFARIVSCYLGDSDDAFRSQVFGFIRTEITRETEAAAAAATAAESPVGGAKNKPKSQSNKHDGGGECAGSAVDELAEKRDVVLSKLPQLMELNKVEAVFTVGEAFRGDHKAALAALAATTTLQFDYLDALLRP------GAAAAGKHSGGAAPS-SRTR--------------------------PPPTLVLAKAKGLDDGDRALHVRLLVRFRPYEVYPFLSSSTGYNLDETLALCQEKGVADATAYLLERKGDVPGALALALRTL-----------------------------------------------STRLVALRAALQAASLRDLAAEMAYFSTAGSGSGFGSDLSSPGKSPFP-----------------QLSPKRLSEGGAGMGSWGV--------------GPRAGTAAWSRWASKVALEGLQEGREAVRSLMVAIEMCQRASPNSAGAPIG---LRAAGAARHHRPAAVGAAGIAGQGGLPSSPAANDDLGDNDGDEGLDLSASLWFQTLDKILSAKSAVCRGGPAFSSLASDGTFAAGGGGSERGASGAAGAGLVDSSGVDDASAASAAGELPQTAAVMGGVLDALLQRTLSSMAGYVPLPAIVKKVVSDHAGSSLGEFRHVILAMLETYSYDTAIHSTAASLLFDSLRAVVRERHKLKGAGVRVAAVAGIELRAPDHGSDGSGANRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAELIDAVGSGVISVSPRGLAVVEHLGRNGGCGGDDGGATVGGRLSGDEVMGDSVRRAAAXXXXXXXXG---------------AAVSLWRLRAARRRRQARGNKMRALVGWAAG--LGPHTGEFIPAPGPGGGGDQANTSIRERSGGRGGSGVNPVL-RAGSALAGRSGALA-----GGDSSRAAGAIYQGGGGLVGDSIGTQEQRLQQQLEEAG------QSDGRQALSWGSALQARAR-ALPASLLPKRT 2467
            S +   +EL+RWR D  G GR   G     PP+  + +A                    + G  G  SV YSD  SG  GSST    D N GS SGA  RTAFLSRDE  EDS LLQQILRESE E+AAS ADRAN     XXXXXXXXX               +MT+  S+VGGAG    DLYGAADDNL SLDVD+II SMELEA  ++D    AG GGS  GM  WRASA                                                                   G  A+         XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX       L        E AELRLLRGGN +++SPLQVKRRMRAP  XXXX         XXXXXXXX V  VRMENL AASRQLARNAAYGKHGPGVC+ATAAHSKFIA+GTSRGLILLFDHFQ          EIRHVLGSA G GA+GAPADDPVTSLDMFVGSD LVAGHGSGRVLLWDYL RGVVLK+VVD H+SAVVSVRCFV GG  G  XXXXXXXXXXX     +PAAITADVEGVV+KMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLP+WTDSA PSP+   ++        G A  G  PGF+DLGMMAMSSWKNTF+VGI+PEVKVIHKWPRP L +                        HLPPPCLSWGP QPSA  A AP MALLARGWGNSIQILAATRV D GG     XXXX          R G RWP LAVVKEL ASA VVAVEWLREQV                                                                                                                                                                                                         GG                            PRSEAAEHISDLLAQYLRLA+NNAP A+ S       XXXXXXXXXXX S GG RINLA+SHFQMLAGVCAEFCAVTGRLDMLFGQIF AFR RGQQGVFLDTLEPYVL GKLKTLSPEVI+AF+DR QS GD I++ERLVLRLEPR L+L TTLPLLR HR+YSALLAV A  GD+VSS EEVL EA+SIAE ++      P   +G            ++ L PYPADP+FDELGYKVLLYL+RCF+GRVLGAAEPL A +TA+AR+SLLHLLVRP+L+Q  SS+   S       G                       RRW+EA +P+L+VLLYVDA A +DVLSVAMDSPDA F+RE P  A    XXXXXXXXXXXXX          P L RE  SSS+S                                                    LG++ RAARAVGW  G+   A G        +G  V+G+  W   G  A A  AE  CPSRR +VEAVTSVLAPHL+  AEQL A +XXXXXXXXXXXXXXXXXXXX                                  G G+          A AAA  SSLLRDRDGQEWWAAARPHLFGFVAKYLELSL+SVGPRLTNAVLVS ATG                    XXXXXX      A DAQ RLVTLLERVPL+ VDR+RLLPLAE+S FFRAAVILLKAAVTLGEQRG+++ QFARIV CYL DS+DAFRSQVFGFIR EI     AAA ++  A S    AK+  +S    H GGGE     +  L+EKRDVVL KLPQLMEL+K EAVFTVGEAF GDHKAALAAL+ATTTLQFDYLDALLRP      G +++    G   P  +R R                          P        A GLDDGDR+LHVRLLVRFRPYEVYPFLSSSTGY+LD+TLALCQE+GVADATAYLLERKGDVPGALALALRTL                                               S RL +LR ALQ ASLR+LAAEMA  S+                                      LS  R  +G                       GPRAGTAAW+RWASKVALEGLQEGREA RSLMVAIEMCQRASP+S+G  +G    R A  ARH   A               SP A+D   D+DG+  LDLSASLWFQTLDKILSAK A CR GP                     A  AA         V       AAGELPQTAAVMGGVLDALLQRTLSSMAGYVPLPAIVKKVVSDHAGSSLGEFRHVILAML+TYSYDTAIH TAASLLF+SLR VVRERH LKGAGVRV+AV G +L  P               XXXXXXXXXXXXXXXXXXXXXXXXXXX           + L+ A+GSGVI VS  G+AVVEHLGR G                              XXXXXXXX                A+ SLWRLRAARRRRQARG+KMR+L  W+AG   GP  G+         GG++A  +I          G  PV+ R GS ++GRSG        G D +   G ++Q G                             QS GRQ L WGSALQARA  ALPAS L KRT
Sbjct:    2 SNVSPSRELERWR-DDGGGGRGA-GTSANLPPVASQGSATAAAAGGLLSPRSFGGEDTSTKGGRGAESVGYSDPYSGGGGSSTY--GDGNNGSLSGAPPRTAFLSRDEVSEDSRLLQQILRESEEEIAASVADRANGFGAGXXXXXXXXX---------------AMTRLVSSVGGAGS---DLYGAADDNLQSLDVDKIIESMELEAGEDDDVTGRAGGGGS--GMTPWRASAXXXXXXXXXXXXXXXXXX-----------------------------------XXXXXXXXXXXXXXGAAASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRASRGSLLSGVAGDAKEAAELRLLRGGNREMISPLQVKRRMRAPPPXXXXGQPSAGFPGXXXXXXXXGVGVVRMENLDAASRQLARNAAYGKHGPGVCTATAAHSKFIAVGTSRGLILLFDHFQVGGGGGRGGVEIRHVLGSAAGGGADGAPADDPVTSLDMFVGSDYLVAGHGSGRVLLWDYL-RGVVLKAVVDVHSSAVVSVRCFVQGGSGGGXXXXXXXXXXXXLAGSSDPAAITADVEGVVSKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPTWTDSASPSPSL--SSXXXXXXRRGVAVGGPGPGFYDLGMMAMSSWKNTFIVGIHPEVKVIHKWPRPPLFSGXXXXXXXXXXXXXXXXXXXXXXGHLPPPCLSWGPSQPSAARAQAPPMALLARGWGNSIQILAATRV-DAGGRGGAGXXXX----------RGGARWPGLAVVKELSASAPVVAVEWLREQVLAYLDTDMELCVLDTRAGTELEHASLSTLSVSISPILPXXXXXXXXXXXXXXXXXXXXXXGTQAAGTRSGPAESTLXXXXXXXPGTYHNAFRACEGRLYLLGRQELRAVRVQGWSQRVESLVQAGEWLEALAVALDHFEQKILPAENAAAXXXXXXXXXXXXXXXXXASAHRADGATGGPLHRSGSSRDIRGVAGRMAANFGGEMLKSSSXXXXXXWPXXXXXXXXXRRPTPRSEAAEHISDLLAQYLRLAMNNAPGADSSSLHSTAAXXXXXXXXXXXGSPGGPRINLAHSHFQMLAGVCAEFCAVTGRLDMLFGQIFQAFRARGQQGVFLDTLEPYVLMGKLKTLSPEVISAFIDRCQSAGDMIAAERLVLRLEPRSLELETTLPLLRGHRLYSALLAVGALTGDHVSSAEEVLREAMSIAERDRLRAPNPPPCGDGGQ---GGEGYRQQQLLHPYPADPTFDELGYKVLLYLKRCFRGRVLGAAEPLSAVDTAKARASLLHLLVRPALMQSSSSSLGAS-------GVAAADAATGGRRMGGSSKPWGGDRRWQEAPFPHLAVLLYVDAGATIDVLSVAMDSPDATFRREVPAAAXXXXXXXXXXXXXXXXXXXXXXXXXVPPTLHREFSSSSSS----------------------------------------------------LGRRGRAARAVGWATGAPVSAGGPETRTKVVEGEEVDGV--W---GGTAAAVEAENLCPSRRSVVEAVTSVLAPHLSDQAEQLEAASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------------GYGDDGLT------AEAAAQQSSLLRDRDGQEWWAAARPHLFGFVAKYLELSLVSVGPRLTNAVLVSVATGXX------------------XXXXXXHEGPDPAVDAQARLVTLLERVPLSLVDRDRLLPLAEKSGFFRAAVILLKAAVTLGEQRGFTLEQFARIVRCYLQDSEDAFRSQVFGFIRQEIAHAATAAAPSSGNASSKPRQAKHGDRSPPGTH-GGGEEGNKEL--LSEKRDVVLEKLPQLMELDKAEAVFTVGEAFGGDHKAALAALSATTTLQFDYLDALLRPPSEQQQGVSSSPSPPGSGGPDIARGRSGWGGGQQGVRHHHRDDRVDVGGVRGPGSRPSSGLAAGLDDGDRSLHVRLLVRFRPYEVYPFLSSSTGYSLDDTLALCQERGVADATAYLLERKGDVPGALALALRTLQNLGRAAGRGGGGXAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSARLASLRTALQKASLRELAAEMALVSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLSSTRFGDGXXXXXXXXXTVGXXXXXXXXXAKGPRAGTAAWARWASKVALEGLQEGREATRSLMVAIEMCQRASPSSSGGSVGSMSARRASGARHRSTAGXXXXXXXXXXXXXRSPLADDF--DDDGE--LDLSASLWFQTLDKILSAKGAACRSGPXXXXXXXXXXXXXXXXXXXXXADPAASVPGXXXXAVAPGRGREAAGELPQTAAVMGGVLDALLQRTLSSMAGYVPLPAIVKKVVSDHAGSSLGEFRHVILAMLDTYSYDTAIHRTAASLLFESLRTVVRERHTLKGAGVRVSAVGGFDLHPP-----------RIAAXXXXXXXXXXXXXXXXXXXXXXXXXXXRSLAPHHPPVPSGLLGALGSGVIRVSSAGVAVVEHLGRGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDASASLWRLRAARRRRQARGDKMRSLARWSAGEDTGPIRGD---------GGERARGTI----------GHIPVMTRGGSKVSGRSGXXXXXXXLGRDEN---GRVHQRGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQSRGRQPLGWGSALQARAALALPASFLTKRT 2608          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: D8LRX0_ECTSI (Vps8 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRX0_ECTSI)

HSP 1 Score: 1300 bits (3365), Expect = 0.000e+0
Identity = 1052/1856 (56.68%), Postives = 1139/1856 (61.37%), Query Frame = 0

Query:   65 IGTKQELDRWRGDHNGAGRSKEGAVKQRPPLGYRDTAAXXXXXXXXXXXXXXXPSPPSYG-------RGGGS-SVAYSDRVSGDEGSSTTTGADCNGGSASGALARTAFLSRDEGLEDSLLLQQILRESEAEVAASAADRANXXXXXXXXXXXXXXXXXXXXXXXXXXKGESMTKFASTVGGAGGXGGDLYGAADDNLHSLDVDRIIASMELEAEAEEDGDLMAGRGGSDGGMPSWRASAAG---LMATMASSSRNRSVTSNGVTEGGGGGRAMASGLRERGASATGGEGRAGGKGVVSLSPPTSNDGAYGVGATDRPDLAGIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRALRKAETAELRLLRGGNTDIVSPLQVKRRMRAPXXXXXXXXXXXXXXXXXXXXXXXXV--VRMENLKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGG--WGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPSWTDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRPQLSTNNVGAGAPSEHLHLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGTRWPALAVVKELGASAQVVAVEWLREQVGGSAPPRSEAAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXX----SSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAVRASRGDYVSSTEEVLLEAISIAEHEQ-------PYIEEGAAEAXXXXXXXXERQLWPYPADPSFDELGYKVLLYLERCFQGRVLGAAEPLPAAETARARSSLLHLLVRPSLLQPGSSASSPSEPHDRSAGFXXXXXXXXXXXXXXXXXVCRRRRRWREAMYPYLSVLLYVDAPAALDVLSVAMDSPDANFQRE-PGPAFGGGXXXXXXXXXXXXXGG-------PKLFRESWSSSTSSVSLLATGAEGPEVSAGGTSDGGGRGNEGESRRRKGSARSGEALGGGVWGRRLGQKVRAARAVGWTVGSAAMAAGKADGDGGGVEGIRKWRRAGLEAVAAAAETSCPSRRMIVEAVTSVLAPHLTADAEQLIAPAXXXXXXXXXXXXXXXXXXXXGDGTPDDGGGLSAAAFVHGNRPAAAPATPAAATAGAGESSTKMAMPTAATAAALSSSLLRDRDGQEWWAAARPHLFGFVAKYLELSLLSVGPRLTNAVLVSAATGTCGGSGGGPVEGGXXXXXXXXXXXXXXXXLGSAGDAQTRLVTLLERVPLAFVDRERLLPLAERSRFFRAAVILLKAAVTLGEQRGYSVSQFARIVSCYLGDSDDAFRSQVFGFIRTEITRETEAAAAAATAAESPVGGAKNKPKSQSNKHDGGGECAGSAVDELAEKRDVVLSKLPQLMELNKVEAVFTVGEAFRGDHKAALAALAATTTLQFDYLDALLRPGA--------------------------------------------AAAGKHSGGAAPSSRTRPPPTLVLAKAKGLDDGDRALHVRLLVRFRPYEVYPFLSSSTGYNLDETLALCQEKGVADATAYLLERKGDVPG 1842
            +   +EL+RWRGD  G G    G      P+  R +A                 SP S+G       RGGG+ SV YSD  SG        G D N GS SGA  RTAFLSRDE  EDS LLQQILRESE E+AAS      XXXXXXXXXXXXXXXX             +MT+  S+VGGAG    DLYGAADDNL SLDVD+II SMELEA  ++D    AG GGS  GM  WRAS AG        A S R RS T++G            S                                                XXXXXXXXXXXXXXXXXXXX                                    ALRKAE AELRLLRGGN +++SPLQVKRRMRAP  XXXX         XXXXXXXX V  VRMENL AASRQLARNAAYGKHGPGVC+ATAAHSKFIA+GTSRGLILLFDHFQEIRHVLGSA G GA+GAPADDPVTSLDMFVGSD LVAGHGSGRVLLWDYL RGVVLK+VVD H+SAVVSVRCFV GGG      XXXXXXXXXXXXXX  +PAAITADVEGVV+KMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLP+WTDSA  S               GGA  G  PGF+DLGMMAMSSWKNTF+VGI+PEVKVIHKWPRP L T            H PPPCLSWGPPQPSA  A AP MALLARGWGNSIQILAATRV D GG     XXXX          R G RWP LAVVKEL ASA VVAVEWLREQ   +  PRSEAAEHISDLLAQYLRLA+NNAPAA+ S    XXXXXXXXXXXXXX     S GG RINLA+SHFQMLAGVCAEFCAVTGRLDMLFGQIF AFR RGQQGVFLDTLEPYVL GKLKTLSPEVI+AF+DR QS GD I++ERLVLRLEPR L+L TTLPLLR HR++SALLAV A  GD+VSS EEVL EA+SIAE ++                 XXXXXXXX   L PYPADP+FDELGYKVLLYL+RCF+GRVLGA+EPL AA+TA+AR+SLLHLLVRP+L+Q  SS+   +      AG                       RRW+EA++PYL+VLLYVDA A +DVLSVAMDSPDA F RE P  A    XXXXXXXXXXXXX         P L RE      SS           E   G     GG           G         GG WG           A    V +    A     +G  V+G+  W   G  A A  AE  CPSRR +VEAVTSVLAPHL+  AEQL A + XXXXXXXXXXXXXXXXXXX                                                         LLRDRDGQEWWAAARPHLFGFVAKYLELSL+SVGPRLTNAVLVS ATG                    XXXXXX      A DAQ RLVTLLERVPL+ VDRERLLPLAE+S FFRAAVILLKAAVTLGEQRG+++ QFARIV CYL DS+DAFRSQVFGFIR EI R   AAA ++    S    AK+   S    H GGGE      + L+EKRDVVL KLPQLMEL+K EAVFTVGEAF GDHKAALAAL+ATTTLQFDYLDALLRP                                                 G    G+ PSS            A GLDDGDR+LHVRLLVR+ P               D+TLALCQE+GVA AT YLLERKG  PG
Sbjct:   11 VSPSRELERWRGD--GGGGRGAGTSANLLPVASRGSATAAAAGGLL--------SPRSFGGEDTSTKRGGGAESVGYSDAYSGXXXXXXXYG-DGNNGSLSGAPPRTAFLSRDEVSEDSRLLQQILRESEEEIAASXXXXXXXXXXXXXXXXXXXXXX-------------AMTRLVSSVGGAGS---DLYGAADDNLQSLDVDKIIESMELEAGEDDDVTGRAGGGGS--GMTPWRASVAGGGVSTTATAWSGRRRSSTADG------------SAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPGGSRASRGSLLSGVAGDAKGSAPGGGGGGELGLTGALRKAEAAELRLLRGGNREMISPLQVKRRMRAPPPXXXXGQPSAGFPGXXXXXXXXGVGVVRMENLDAASRQLARNAAYGKHGPGVCTATAAHSKFIAVGTSRGLILLFDHFQEIRHVLGSAAGGGADGAPADDPVTSLDMFVGSDYLVAGHGSGRVLLWDYL-RGVVLKAVVDVHSSAVVSVRCFVQGGGGXXXXXXXXXXXXXXXXXXXSSDPAAITADVEGVVSKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPTWTDSA--SSXXXXXXXXXXXXLRGGAVGGPAPGFYDLGMMAMSSWKNTFIVGIHPEVKVIHKWPRPPLFTGXXXXXXXXXG-HFPPPCLSWGPPQPSAARAQAPPMALLARGWGNSIQILAATRV-DAGGRGGAGXXXX----------RGGARWPGLAVVKELSASAPVVAVEWLREQRRPA--PRSEAAEHISDLLAQYLRLAMNNAPAADSSSRHSXXXXXXXXXXXXXXXXXAGSPGGPRINLAHSHFQMLAGVCAEFCAVTGRLDMLFGQIFQAFRARGQQGVFLDTLEPYVLMGKLKTLSPEVISAFIDRCQSAGDMIAAERLVLRLEPRSLELETTLPLLRGHRLHSALLAVGALTGDHVSSAEEVLREAMSIAERDRLRAPRPPXXXXXXXXXXXXXXXXXXXLLLHPYPADPTFDELGYKVLLYLKRCFRGRVLGASEPLSAADTAKARASLLHLLVRPALMQSSSSSLGAAGVAAADAGGRRAGGSKRPWGGD---------RRWQEALFPYLAVLLYVDAGATIDVLSVAMDSPDATFHREVPAAAXXXXXXXXXXXXXXXXXXXXXXXAVPPTLHRE-----LSSXXXXXXXXXDRERGGG---PAGGEAXXXXXXXXXGEXXXXXXXRGGRWGGGANXXXXXXXAALDIVLAGGTEARTKVVEGEEVDGV--W---GGTAAAVEAENLCPSRRSVVEAVTSVLAPHLSDQAEQLEAASSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----------------XXXXXXXXXXXXXXXXXXXXLLRDRDGQEWWAAARPHLFGFVAKYLELSLVSVGPRLTNAVLVSVATGXX------------------XXXXXXHEGPDPAVDAQARLVTLLERVPLSLVDRERLLPLAEKSGFFRAAVILLKAAVTLGEQRGFNLEQFARIVRCYLQDSEDAFRSQVFGFIRQEIARAATAAAPSSGNTSSKPRQAKHGDGSPPGTHGGGGE---GEAELLSEKRDVVLEKLPQLMELDKAEAVFTVGEAFGGDHKAALAALSATTTLQFDYLDALLRPPTDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRDDRVGVGGVRGPGSRPSS----------GLAAGLDDGDRSLHVRLLVRYSP---------------DDTLALCQERGVAHATPYLLERKGRRPG 1724          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: A0A1Z5K8H4_FISSO (Vps8 domain-containing protein n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K8H4_FISSO)

HSP 1 Score: 242 bits (618), Expect = 2.040e-60
Identity = 249/939 (26.52%), Postives = 363/939 (38.66%), Query Frame = 0

Query:  442 ELRLLRGGNTDIVSPLQVKRRMRAPXXXXXXXXXXXXXXXXXXXXXXXX----VVRMENLKAASRQLARNAAYG-KHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCF-RRVMWTKWVVDTECLLDGAAGPIPALCVLPSW----TDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRP------QLSTNNVGAGAPSEHLHLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGTRWPALAVVKELGASAQVVAVEWLRE------------------------------------------------------QVGGS---------------------------------------------------------------------------------APPRSEAAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXXSSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAV-RASRGDYVSSTEEVLLEAISIAEHEQPYIEEGAAEAXXXXXXXXERQLWPYPADPSFDELGYKVLLYLERCFQGRVLGAA-EPLPAAETARARSSLLHLLVRPSLLQPGSSASSPSEPHDRSAGFXXXXXXXXXXXXXXXXXVCRRRRRWREAMYPYLSVLLYVDAPAALDVLSVAMDSPDA 1227
            E +LL+ G  ++VSPL+VKRR++                              +V  + L   S  L +++  G K   G+ +  A +SKFIA+GT RG++L++D F+ +R  LG+     A GA    PVTS+D+    + +++GH SG ++LWD + RG+VL++V D H S + S R       W                       +T DV G+VNK+ F + ++W+ + VDTECLLDG AG I A+ VLP +    T    P P                           L ++A+SS +++F V + P++ V+H+W RP       +S N       SE L    PCLSWG      P  G   M +LAR WG  +Q+L A+                           +   WPA  V KE+     VVAVEWL +                                                      QV  S                                                                                 A    +  E ++ LL +YL LA+ NAP                       S  GG+  +L+ SH+QMLAGVC EFC+VT + ++LFG +F  F+  G   +F D LEPYVL  K+  +S E ++ FV+  +     ++ ER +L ++  ++D  T L LLRR+ MY+AL  V      DYVS  E VL          + + E  A  A          Q         F+ LGYK LLYL+ CF G+      E LP  +  + R  LL  L           +S   +     AG                          R   YPY+ +LL VDA A LD +++A+++ D+
Sbjct:  243 EQKLLKAGQREMVSPLRVKRRLKQKIELTTRGKEPKKATRIAALSVTRFGSSGLVENKTLTGVSTSLEKHSKEGAKVLCGLPTCLAFNSKFIAVGTQRGIVLVYDLFEVLRQRLGATYDDNA-GAMRAGPVTSIDLSFQGETVLSGHASGILVLWDTI-RGIVLRAVHDTHISPISSARFMSD---W---------------------KVVTVDVGGIVNKISFTKNMLWSNYSVDTECLLDGTAGQILAMNVLPPYGTIKTQQLRPDPLTDM--------------------LRKLTLIALSSERSSFAVAVEPQINVLHRWARPPAEKTDDVSINTSNNPVQSEFL----PCLSWG--WGLIPGGGNTVMPILARAWGCCLQLLCAS---------------FPSIDDKSLIESNSIHWPAFGVHKEVDTGKPVVAVEWLNDRSLMYLTLTNEFTLVDTVMMTLLEKLDFSGLKLVYAEFALSRSSRTPGSDDENGQVSASTFQNSIRYSDDRLLVLCKNELKCISVVGARKRISSLEQDGEWLEALALALDYYDSAVVSLEDRRRDPSRKRDLSRHPDLFRAKGEDDEEEWLAKLLVRYLNLAVENAPDVS------------HIPASARVSINGGN--DLSRSHYQMLAGVCVEFCSVTRKPELLFGPVFQKFQSAGFSSIFFDVLEPYVLNDKIPYISAEAMSYFVEHCKMTKSILTVERCLLHMDCSIMDFDTILSLLRRNAMYTALFYVYNNGLDDYVSPLEIVL---------NRVFEEADAGSALLKRREDGVPQT-------DFETLGYKALLYLKSCFIGKTFPREDELLPDEKCKKVRQDLLTFLASKKY-----QSSFAKKDKQEIAGH-------------------------RALNYPYMRLLLLVDARATLDAVALALNTEDS 1054          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: B7FQM2_PHATC (Predicted protein n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7FQM2_PHATC)

HSP 1 Score: 240 bits (613), Expect = 8.280e-60
Identity = 243/940 (25.85%), Postives = 359/940 (38.19%), Query Frame = 0

Query:  433 RALRKAETAELRLLRGGNTDIVSPLQVKRRM--------RAPXXXXXXXXXXXXXXXXXXXXXXXXVVRMENLKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCF-RRVMWTKWVVDTECLLDGAAGPIPALCVLPSWTD-SAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRP---QLSTNNVGAGAPSEHLHLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGTRWPALAVVKELGASAQVVAVEWLRE---------------------------------------------QVGGSAPPRS-------------------------------------------------------------------------------------------EAAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXXSSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAV-RASRGDYVSSTEEVLLEAISIAEHEQPYIEEGAAEAXXXXXXXXERQLWPYPADPSFDELGYKVLLYLERCFQGRVLGAAEPL-PAAETARARSSLLHLLVRPSLLQPGSSASSPSEPHDRSAGFXXXXXXXXXXXXXXXXXVCRRRRRWREAMYPYLSVLLYVDAPAALDVLSVA 1221
            RAL  A   E +LLR G+ +I+SPL VKRR+        R+                         V   E L   +  +    A      G+ +  A +S+FIAIGT  G++L++D F+ +R  LG  G      A     VTSLD+    D +++G+ SG V+LWD + +G VL+S+ D H S + +VR FV                           A+T D  G+VNK+ F + ++W+ +  D +CLLDG AG I ++ VLP +   +    P  LA+  RG                  + ++A+SS +++F+V + P V V+H+W RP    L+ + V       H H+P  CL+WG         G   M +LARGWG  +Q+L  +                           +   WPA  +  E+ A A ++A+EWL                                               Q G     R                                                                                            E  E I+ LL +YL LA+ NAP +  +                       +RI+LA SHFQMLAGVC +FC VT RLD+LFG IF  F+  G   +FLD LEPYVL  +L  ++PEV+  FV+  +S     + ER +L ++   +D  T + LL  + M++AL  V      D+V+  + +          E+ + E    +A          Q         F+  GYK +LYL+ CF+G+     E + P       +  LL LL+R           +PS PH   +G                          R  +YPY+ + L VD  AALD LS+A
Sbjct:  244 RALEHARAYEQKLLRAGHREIISPLMVKRRLKPKIELCSRSARKKRPQLTERFASTSTPRFNFSGIVEEKEMLLIGASLVQHTTAGSDENYGLPTCLAFNSRFIAIGTQLGIVLVYDFFEVLRQQLGGGGSEDNWNAQKAGAVTSLDLSTNGDMIISGYTSGCVVLWDAI-KGTVLRSIHDIHPSPITTVR-FVA-----------------------NLKAVTVDAGGLVNKVAFVKNILWSTYSTDIDCLLDGTAGQILSMNVLPPYAMVNPQLRPENLASVLRG------------------MNLIALSSERSSFVVAVDPTVNVLHRWARPPQEDLNESKVDDHEREVHAHMP--CLAWG--WALTSGGGNLVMPVLARGWGCCLQLLGVS------------FPTGDDTTNVEKGKNAPIHWPAFGLHDEIDAKAPILALEWLSSRSVLYLTLSNELHLIDTVMMTLLERIDVSNLRTVYAEFSLSRNASQKGDRIASRDNDLEVSVTFQNSVRCSHDRLMILCQEQLKCISIVGARRRISCLEADGEWLEALALTLDHYESTVISQEDRQRNPNQAKDFASRRNFVFTKGEDHEWIAKLLMRYLNLAVENAPDSSANQPWSPGSID--------------ARIDLAQSHFQMLAGVCVDFCVVTRRLDLLFGPIFRRFQHVGYTDIFLDVLEPYVLNDRLTYIAPEVMNHFVEYCRSHNGIATVERCLLHMDCTAMDFDTIISLLMVNEMFTALFFVFNQGLNDFVTPLQMLF---------EKLFEEADTGKALLSRRRNGTPQ-------NRFERYGYKAILYLQTCFRGKTFPVEESIVPDERRGSLKRELLELLMRKDY--------NPS-PHSMHSGENNVVIGQ------------------RAQLYPYMKIFLQVDPRAALDSLSLA 1067          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: A0A662WX84_9STRA (Chromo domain-containing protein n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WX84_9STRA)

HSP 1 Score: 236 bits (601), Expect = 2.800e-58
Identity = 422/1767 (23.88%), Postives = 622/1767 (35.20%), Query Frame = 0

Query:  435 LRKAETAELRLLRGGNTDIVSPLQVKRRMRA--------------------PXXXXXXXXXXXXXXXXXXXXXXXXVVRMENLKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPSWTDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRPQLSTNNVGAGAPSEHLHLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGTRWPALAVVKELGASAQVVAVEWLREQVG-------------------------------------------------------------------------------------------------------------------------------------------------------------GSAPPRSE-------------------------AAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXXSSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAVRASRGDYVSSTEEVLLEAISIAEHEQPY-------------------IEEGAAEAXXXXXXXXERQLWPYPADPSFDE---------------LGYKVLLYLERCFQGRVLGAAEPLPAAETARARSSLLHLLVRPSLLQPGSSASSPSEPHDRSAGFXXXXXXXXXXXXXXXXXVCRRRRRWREAMYPYLSVLLYVDAPAALDVLSVAMDSPDANFQREPGPAFGGGXXXXXXXXXXXXXGGPKLFRESWSSSTSSVSLLATGAEGPEVSAGGTSDGGGRGNEGESRRRKGSARSGEALGGGVWGRRLGQKVRAARAVGWTVGSAAMAAGKADGDGGGVEGIRKWRRAGLEAVAAAAETSCPSRRMIVEAVTSVLAPHLTADAEQLIAPAXXXXXXXXXXXXXXXXXXXXGDGTPDDGGGLSAAAFVHGNRPAAAPATPAAATAGAGESSTKMAMPTAATAAALSSSLLRDRDGQEWWAAARPHLFGFVAKYLELSLLSVGPRLTNAVLVSAATGTCGGSGGGPVEGGXXXXXXXXXXXXXXXXLGSAGDAQTRLVTLLERVPLAFVDRERLLPLAERSRFFRAAVILLKAAVTLGEQRGYSVSQFARIVSCYLGDSDDAFRSQVFGFIRTEITRETEAAAAAATAAESPVGGAKNKPKSQSNKHDGGGECAGSAVDELAEKRDVVLSKLPQLMELNKVEAVFTVGEAFRGDHKAALAALAAT----TTLQFDYLDALLRPGAAAAGKHSGGAAPSSRTRPPPTLVLAKAKGLDD-GDRALHVRLLVRFRPYEVYPFLSSSTGYNLDETLALCQEKGVADATAYLLERKGDVPGALALALRTLSTRLVALRAALQAASLRDLAAEMAYFSTAGSGSGFGSDLSSPGKSPFPQLSPKRLSEGGAGMGSWGVGPRAGTAAWSRWASKVALEGLQEGREAVRSLMVAIEMCQRAS 1960
            L  AE  E +LL     +++SPLQVKRR+RA                          XXXXXXXXXXXXXX     VV++E ++A SRQL +N  + ++GPG  +  A HSKFIAIGTS+GL+++FDHFQ IR VLG+        A +D P+T++D+  GSD LV G+ SGR++LWD + +G  LK+V DAH + VVS+R                           +P  ++ D  G+VNK+ F ++M   +VVD + L DG+AG I ++ VLP    +A  +                              + A+SS K TF++ I PEV+V+++W RP    +++ A  P     LP    +W     S P +      +LARGWGN +Q L    VV  GG                   R G  +P      ++  S+ V+AV+WL +QV                                                                                                                                                              G  P  S+                          AE ++DLL +Y+RLAI NAP +  +                      G +++LA SH+QMLAGVC E+CA+ GR D+LFG+IF  F+  G+ GVF++ LEPY+L+ KL++LSP  +  FV  F ++G     E+ +L L    LDL T L L   H +YSAL+ +     D  ++  +VLLEA S A+  +P                    +   A+           +     P   S  E                GYK+LLY+     GR     EP+   +  + RS + + L   ++      A++P                                       +P L  L+ +DA    +V++   DSP+  F+ E                           ++  S+S+   S                              R  SAR+ E                                                             T CPSR  IV ++           AE +  P                            GG +    +      A   A  A  ++G                                 A+    L  F+A     SLLS G        V+ AT           EG                    AG  +  LV LL ++  A  + E LL    R +  RAAV+L K       ++G     FA+ ++ YL D D  ++   F +IR E  +  +         E     + +   S        G   G    ++ E  + VL     LM+ +    V  +   F   +   +    +       L+F YL  +L     AA   SG +A          L  +K +  DD   +   VRLL  F P  V+PFL S   Y +D  L LC+E G+ DA AYLLER GDV GAL L L++L  +L  L+ A     LR   A     ++AG+     SD+               +   G   G+   G    ++A    +S   ++ + EG++A+++L VA+ MCQR S
Sbjct:  403 LEIAEAREKQLLSCVGVELISPLQVKRRLRAHARSKALKSRRPESAVMNAKAKSQRKXXXXXXXXXXXXXXKKSTGVVKVEPMEAISRQLRKNIEFKEYGPGSPTVVAIHSKFIAIGTSKGLVIIFDHFQNIRQVLGNT-----TDADSDGPITAVDVSPGSDYLVCGYQSGRIVLWDMI-KGTSLKAVSDAHENPVVSLRFL----------------------KDQKPVLVSVDTNGLVNKLNFSKMMGMVYVVDVDPLYDGSAGKILSISVLPQSAGNAKIAYLT-----------------------DQYCLAALSSEKVTFIIAIEPEVRVLYRWTRP----DDIAADDPV----LPSLAFAWI----SFPGSSRALAPVLARGWGNRVQFL---EVVFPGGKGHTHA-------------RHG--FPTFDEHDQIECSSDVMAVQWLGDQVVVYLNSHDEICVYDVMSRQELEMVDVSSLELVFASYRGKNARSFSNSFRGCYNILYLLGLKELQTARVQPWTQRIDSLVDEGEWLEALALALDHYEGLKIAAADRATRDRFPPVFFRDKQNDQCLVDILHMCQTNQRTGEKEDVFRHEESAEEVRWVCGEVPYPSDIAKKLEETLQKARSGEVTKNFVPISVAERVADLLMEYVRLAIANAPGSTSAATGELGLSKI------------GMKLDLAKSHYQMLAGVCIEYCALIGRTDLLFGEIFTRFKEAGKLGVFVELLEPYILSEKLRSLSPVAMQEFVKHFSAQGKLAQVEQCLLHLNVAELDLDTILKLCHDHELYSALIYIYNEGLDDYTTPIDVLLEACSDAKKAKPKPAVEHTPAPRLARAPSGSRVSSLASAFGGTTRTASSKVAISKPVGLSAQEKAEEEELSGPRRRRLYGYKLLLYISYALSGRTFPKHEPIATQKLGKVRSQICYHLFEKTVAM----ATNPRP-------------------------------------FPRLETLIDLDARELFNVMARMFDSPNIEFEGE---------------------------KKDGSTSSRPTS------------------------------RYDSARNAEL------------------------------------------------------------TKCPSRLSIVISL-----------AEVIFGPKSPFSSVEHAHFFMFEARLL-------SGGSIEPQEYADARSEAIGDAGAAGGSSG---------------------------------ASMMDSLMNFLALG-PASLLSKGSSA-----VTPATSQ--------EEG-----------------FDKAG-REAMLVRLLLKLNKATYNHEALLASVIREKMNRAAVLLYK-------EKG----DFAQAIASYLADEDHEYQMNAFSYIRVETDKAVDG--------EDLEHSSHDSLSSXXXXXXXTGAGPGRRRRKVIE--EAVLVHAGALMKTDDYAFVTLILGQFPNLNNKVIQRFLSMGKEGAQLEFYYLKQVLM----AASITSGSSASDEADVVKDLLDRSKLRIADDPAVQERFVRLLCEFEPVGVFPFLESHDSYKVDACLRLCKEFGITDAEAYLLERTGDVTGALTLILQSLEQKLKILKPA-----LRGYNASSVSSTSAGA-----SDM--------------LVGSSGVNGGTGSAGALTASSAARHQSSDRIIDSVAEGQDAMKTLEVALAMCQRNS 1786          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: A0A3F2RY97_9STRA (Vps8 domain-containing protein n=22 Tax=Peronosporaceae TaxID=4777 RepID=A0A3F2RY97_9STRA)

HSP 1 Score: 233 bits (595), Expect = 1.140e-57
Identity = 265/1036 (25.58%), Postives = 389/1036 (37.55%), Query Frame = 0

Query:  435 LRKAETAELRLLRGGNTDIVSPLQVKRRMRA-----------------PXXXXXXXXXXXXXXXXXXXXXXXXVVRMENLKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPSWTDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRPQLSTNNVGAGAPSEHLHLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGTRWPALAVVKELGASAQVVAVEWLREQV-------------------------------------GGSA----------------------------------------------------------------------PP--------------------------------RSE-------------------------------------------AAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXXSSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAVRASRGDYVSSTEEVLLEAISI--------AEHEQPYIEEG---------AAEAXXXXXXXXERQLWPYPADPSFDE---------------LGYKVLLYLERCFQGRVLGAAEPLPAAETARARSSLLHLLVRPSLLQPGSSASSPSEPHDRSAGFXXXXXXXXXXXXXXXXXVCRRRRRWREAMYPYLSVLLYVDAPAALDVLSVAMDSPDANFQREPGPAFGG 1239
            L  AE  E +LL     +++SPLQVKRR+RA                                          VV++E ++A SRQL +N  + ++GPG  +  A HSKFIA GTS+GL+++FDHFQ IR VLG+      N A +D PVT++D+  GSD LV G+ SGR++LWD + +G  LK+V DAH + VVS+R                           +P  ++ D  G+VNK+ F ++M   +VVD + L DG+AG I ++ VLP    +A  S                              + A+SS K TF++ I PEV+V+++W RP    +++   AP + + LP    +W     S P +      +LARGWGN +Q L    VV  GG                   R G  +P      ++ +S+ V+AV+WL +QV                                     G +A                                                                      PP                                R E                                            AE ++DLL +Y+RLAI NAP +  +                      G +++LA SH+QMLAGVC EFCA+ GR D+LFG+IF  F+   +  VF++ LEPY+L+ KL+ LSP  +  FV  F ++G     E+ +L L    LD+ T L L   H +YSAL+ +     D  ++  +VLLEA S         A    P +            A+           +     PA+ S  E                GYK+LLY+     GR     EP+ A +  R RS + + L   ++     S SS + P+ R                                    L  L+ +DA    +++   +DSP+  F+ E     GG
Sbjct:   97 LEIAEAREKQLLSCVRVELISPLQVKRRLRAHARSKALKSRHDGVVTTKKNERKKKIAEGTAVNANAEKKSTGVVKVEPMEAISRQLRKNIEFKEYGPGSPTVVAIHSKFIATGTSKGLVIIFDHFQNIRQVLGNT-----NDADSDGPVTAVDVSPGSDYLVCGYQSGRIVLWDMI-KGTSLKAVSDAHENPVVSLRFL----------------------KDQKPVLVSVDTNGLVNKLNFSKMMGMVYVVDVDPLYDGSAGKILSISVLPQSAGNAKISYLT-----------------------DQYCLAALSSEKVTFIIAIEPEVRVLYRWARP----DDI---APDDPV-LPSLAFAWI----SFPGSSRALAPVLARGWGNRVQFL---EVVFPGGKNHSHG-------------RHG--FPTFDEHDQIESSSAVMAVQWLGDQVVVYLNSHDEICVYDVMSRQELEIVDVSSLELVFASYRGKNARSFSNSFRGCYNILYLLGLKELQTARVLPWTQRIDLLVDDGEWLEALALALDHFEGLKVAAADRAARDRFPPVFFRDKQNDQCLVDILHMCQTNQRTGEKEDVFRHEESADEVRWVCGEVPYPPDIAKKLEETLQKARSGEVTKNFVPISVAERVADLLMEYVRLAIANAPGSTAATSGELGLNKI------------GMKLDLAKSHYQMLAGVCIEFCALIGRTDLLFGEIFTRFKEANKLSVFVELLEPYILSEKLRNLSPVAMQEFVQHFSAQGKLAQVEQCLLHLNVAELDMDTILKLCHDHELYSALVYIYNEGLDDYTTPIDVLLEACSDEKASKSTPASDPSPRVTRAPSGSRVSSLASTFGGTTRAAGSKISIAKPAELSTREKAEEEALTGPRRRRLYGYKLLLYISYALSGRTFPKHEPIAAQKLGRVRSQICYHLFEKAV-----SMSSHARPYAR------------------------------------LETLIDLDARELFNIMGRILDSPNVEFEGEKKDEVGG 998          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: A0A2D4BIS2_PYTIN (Vacuolar protein sorting-associated protein 8 n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BIS2_PYTIN)

HSP 1 Score: 233 bits (593), Expect = 2.680e-57
Identity = 237/845 (28.05%), Postives = 341/845 (40.36%), Query Frame = 0

Query:  497 LKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPSWTDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRPQLSTNNVGAGAPSEHLHLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAAT-------------------------------------------------------------RVVDVG--------------------------------GXXXXXXXXXXXXXXXXXXXRSGTRW-PALAVVKELGASAQVVAVE---------------------------------------------------W--------------LRE--------QVGGSAPPRSEAAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXXSSGGGS------RINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAV-RASRGDYVSSTEEVLLEAISIAEHEQPY-------------IEEGAAEAXXXXXXXXERQLWPYPADPSFD---------ELGYKVLLYLERCFQGRVLGAAEPLPAAETARARSSLLHLL 1145
            + A SRQL +N  + +HGPG  +  A HSKFIAIGTS+GL+L+FDHFQ IR VLG+      N A +D PVT++D+  GSD LV G+ SGR++LWD + +G  LK+V DAH   VVS+R                           +P  ++ D  GV+NK+ F ++M   +VVD + + DG+AG + ++ +LP    +A  S                              + A+SS K TFL+ I PEVKV+++W RP    ++VGA  P     LP    +W     S P +      +LARGWG  +Q L                                                                 VVDV                                 G                       +W  ALA+  +     +V AV+                                                   W              L E        QV  +  P S  AE ++DLL +Y+RLAI NAP++                     S+GG S      +++L+ SH+QMLAGVC E+CA+ GR D+LFG+IF  F+  G+  VF++ LEPY+L+ KL+TLSP+V+ AFV  +         E+ +L L+   LD  T L L ++H +YSAL+ +   +  D+V+  + +LL+A+  A    P              +   AA      XXXX        +D + D         + GYK+LLYL     GR     EPLP    ++ R+ +LH L
Sbjct:    1 MDAISRQLQKNLEFKEHGPGSPTVVAIHSKFIAIGTSKGLVLIFDHFQNIRQVLGNT-----NDADSDGPVTAIDVSPGSDYLVCGYQSGRLVLWDMI-KGTSLKAVSDAHEHPVVSLRFL----------------------KDQKPVLVSVDTNGVLNKINFSKMMGMVYVVDVDPIYDGSAGKLLSISILPQSAGNAKISHLT-----------------------DQYVLAALSSEKVTFLIAIEPEVKVLYRWARP----DDVGADDPV----LPSLAFAWI----SFPGSSRALAPVLARGWGRHVQFLEVVFPGGQHHSHSKHGWPTFEEHDLQIETASDTTALQWLGDQVIVYLNAHDEICVYDVMSRQELEVVDVSLLELVFASYRGKSNARSFANSFRGCDNILYLLGLKELQTARVQPWTQRIDTLVDDGQWLEALALALDHYEGLKVAAVDRAVRDRFPPVFFRDRNNDQCLVDLLHMSQTNQRTGEKEDVFRHEDSADEVRWRCGEELYAPDVAQRLEETYQKARSGQVTKNFVPIS-VAERVADLLMEYVRLAIANAPSS---------------------STGGSSIGRSQSKLDLSKSHYQMLAGVCIEYCAIIGRTDLLFGEIFKRFQDAGKTDVFVELLEPYILSDKLRTLSPDVLEAFVSHYDRHDKLAQVEQCLLHLQVAELDTDTILSLCKKHELYSALVYIYNEAHQDFVTPID-ILLDAMKTATPPAPKPAPITTRAPSGSKVSSLAAAFGGTAXXXXXXXXTLAKSDAADDPEATARRRRQYGYKLLLYLSYSLSGRSFPKHEPLPPQLLSKVRADVLHHL 759          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: D8LRX1_ECTSI (Vps8 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRX1_ECTSI)

HSP 1 Score: 214 bits (545), Expect = 3.420e-57
Identity = 228/408 (55.88%), Postives = 252/408 (61.76%), Query Frame = 0

Query: 2095 MGGVLDALLQRTLSSMAGYVPLPAIVKKVVSDHAGSSLGEFRHVILAMLETYSYDTAIHSTAASLLFDSLRAVVRERHKLKGAGVRVAAVAGIELRAPD-HGSDGSGANRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXA--------ELIDAVGSGVISVSPRGLAVVEHLGRNGGCG------GDDGGATVGGRLSGDEVMGDSVRRAAAXXXXXXXXG-AAVSLWRLRAARRRRQARGNKMRALVGWAAG--LGPHTGEFIPAPGPGGGGDQANTSIRERSGGRGGSGVNPVL-RAGSALAGRSGALAGG--------DSSRAAGAIYQGGG-------GLVGDSIGTQEQRLQQQLEEAGQSDGRQALSWGSALQARAR-ALPASLLPKRT 2467
            MGGVLDALLQRTLSSMAGYVPLPAIVKKVVSDHAGSSLGEFRHVILAML+TYSYDTAIH TAASLLF+SLR VVRERH LKGAGVRV+AV G +L  P    +DG    R    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  A         L+ AVGSGVISVS  G+AVVEHLGR            D+G                       XXXXXXXX  A+ SLWRLRAARRRRQA+G+KMR+L  W+AG   GP  G+         GGD+A  +I          G  PV+ R G+ ++GR+G   GG        D +   G ++Q GG                             QS GRQAL WGSALQARA  ALPAS L KRT
Sbjct:    1 MGGVLDALLQRTLSSMAGYVPLPAIVKKVVSDHAGSSLGEFRHVILAMLDTYSYDTAIHRTAASLLFESLRTVVRERHTLKGAGVRVSAVGGFDLHPPRIAAADGPTRTRNASAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLALHHPPVPSGLLGAVGSGVISVSSGGVAVVEHLGRGXXXXXXXXXXNDEGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASASLWRLRAARRRRQAKGDKMRSLARWSAGEDTGPTRGD---------GGDRARGTI----------GHIPVMNRGGTKVSGRNG---GGVXXXXXXXDEN---GRVHQRGGPGXXXXXXXXXXXXXXXXXXXXXXXXXXXQSRGRQALGWGSALQARAALALPASFLAKRT 383          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: A0A7S3PW51_9STRA (Hypothetical protein n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3PW51_9STRA)

HSP 1 Score: 231 bits (588), Expect = 8.400e-57
Identity = 251/959 (26.17%), Postives = 371/959 (38.69%), Query Frame = 0

Query:  434 ALRKAETAELRLLRGGNTDIVSPLQVKRRMRAPXXXXXXXXXXXXXXXXXXXXXXXX-------VVRMENLKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADD--PVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCFRRVM-WTKWVVDTECLLDGAAGPIPALCVLPSWTDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRPQLSTNNVGAGAP------SEHLHLPPP--------CLSWGPPQPSAPSAGAPSMA-LLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGTRWPALAVVKELGASAQVVAVEWL------------------------------------------------------------------------------REQVG-----------------------------------GSAPPR----------------------SEAAEHISDLLAQYLRLAINNAP--AAEPSXXXXXXXXXXXXXXXXXXSSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAVRASRGDYVSSTEEVLLEAISIAEHEQPYIEEGAAEAXXXXXXXXERQLWPYPADPSFDELGYKVLLYLERCFQGRVLGAAEPL-PAAETARARSSLLHLLVRPSLLQPGSSASSPSEPHDRSAGFXXXXXXXXXXXXXXXXXVCRRRRRWREAMYPYLSVLLYVDAPAALDVLSVAMDSPDANF 1229
            +L+ AE  E RLLR G  DIVSPL VKRRM+                                 ++    ++    +L RN        G+ +A +  SKFIAIGT RG++L+FD F+E+R  L       A  +  +D   ++S+ M    + +VAG+ +G ++LWD + +G +LK+V D H+S +  VR F+                          + ++ D  G+VNK+ F + M ++ +  + ECLLDG AG I A+ +L S   S    P  ++   +                 H L ++A+SS +++F + I P V V+H+W RP     NV +  P      S     PPP        CL+WG    +  S G  S+  +LARGWG  +Q+L                                  WPA  V  E    A VVA+EWL                                                                              +E+V                                     S   R                      +E  E I+DLL +YL LA+ NAP  A EPS                      G+RI+LA SH+QMLAGVC EFC VT RLD+LF +I+  F   G   +FLD LEPYVL  K+K ++P V+A F+D  +   D  + ER +L ++  ++D  + L LL+++ MYSAL+ +     D   S   +L E +  A  +   +  G A                          GYK+LL+++ CFQG++     PL P       R  LL  L+R        ++S+ S+P +                           + +R   YPYL VL+ +D+ A    L++  D PDA F
Sbjct:  435 SLKTAEEYERRLLRPGQRDIVSPLMVKRRMKPKIELNTKSRCNDMHQKRSRITPQQNAQFNFSEMIETSTMQRIGVELKRNVQCNNEVVGLPTAISIGSKFIAIGTQRGIVLVFDLFEELRQKLDVRSENTAASSRENDYGSISSIGMAANGENVVAGYTNGSIVLWDVI-KGTILKTVSDLHSSPITVVR-FIS---------------------EKALSVVSVDAGGLVNKITFVKAMLFSTYTTENECLLDGTAGQILAVDIL-SPISSLKTIPMRISDNKK----------QSYHSSIHRLVLLALSSERSSFGIAIEPTVSVLHRWARPTPEQMNVDSFVPPTSEIDSNDAKPPPPAFPSTFLPCLTWGW---ALVSGGENSVTPILARGWGCFVQLLRVN------------------FPPCDDKSDDAMLWPAFGVHDEFEVKAPVVALEWLGNRSLVYLSLTNELTIVDTVMMTLVERLDFSGMKLVYAEFALSRSITAKSTSSTANNCVSTTFQNSIQSSEERLIVLCQEEVKTVSILGIQQRIVALEESGEWLEALALALDHYESTIASQEDRKRNSDGSFDLSDHPEFVSRIRLTEDEEWIADLLLRYLHLAVENAPDPAVEPSSPFHRR----------------GARIDLAQSHYQMLAGVCIEFCVVTRRLDLLFNEIYQRFLDTGYTWIFLDVLEPYVLNDKVKYIAPLVMADFIDHCREANDVATVERCLLHMDVTIMDFDSILSLLQKNSMYSALIYIYTQGLDDFVSPLIILFEGLFDA-LDSGDVSNGKA-------------------------YGYKILLFMQHCFQGKLFPTGSPLMPEDRLNTLRPELLTCLIRKVY-----TSSAISQPMNDIG-----------------------TKGYRLEAYPYLRVLVNLDSKAFFSTLALVFDDPDACF 1268          

BLAST of mRNA_P-fluviatile_contig52.11545.1 vs. uniprot
Match: A0A0P1AJW9_PLAHL (Vacuolar assembly/sorting protein VPS8 n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AJW9_PLAHL)

HSP 1 Score: 230 bits (586), Expect = 1.160e-56
Identity = 243/929 (26.16%), Postives = 353/929 (38.00%), Query Frame = 0

Query:  435 LRKAETAELRLLRGGNTDIVSPLQVKRRMRA----------------PXXXXXXXXXXXXXXXXXXXXXXXXVVRMENLKAASRQLARNAAYGKHGPGVCSATAAHSKFIAIGTSRGLILLFDHFQEIRHVLGSAGGTGANGAPADDPVTSLDMFVGSDCLVAGHGSGRVLLWDYLSRGVVLKSVVDAHASAVVSVRCFVPGGGWGSGXXXXXXXXXXXXXXXXEPAAITADVEGVVNKMCFRRVMWTKWVVDTECLLDGAAGPIPALCVLPSWTDSAMPSPTALAAAARGGDGAMGGAAAGTPPGFHDLGMMAMSSWKNTFLVGIYPEVKVIHKWPRPQLSTNNVGAGAPSEHLHLPPPCLSWGPPQPSAPSAGAPSMALLARGWGNSIQILAATRVVDVGGXXXXXXXXXXXXXXXXXXXRSGT-----RWPALAVVKELGASAQVVAV---------------------------------------------------------------------EWLR--------------------------------------------------------------------EQVGGSAPPRSE-------------------------AAEHISDLLAQYLRLAINNAPAAEPSXXXXXXXXXXXXXXXXXXSSGGGSRINLAYSHFQMLAGVCAEFCAVTGRLDMLFGQIFHAFRIRGQQGVFLDTLEPYVLTGKLKTLSPEVIAAFVDRFQSRGDHISSERLVLRLEPRLLDLATTLPLLRRHRMYSALLAVRASRGDYVSSTEEVLLEAIS--IAEHEQPYIEEGA--------------AEAXXXXXXXXERQLW-PYPADPSFDE---------------LGYKVLLYLERCFQGRVLGAAEPLPAAETARARSSLL-HLLVR 1147
            L+ AE  E +LL     +++SPLQVKRR+RA                                         VV++E ++A SRQL +N  + ++GPG  +  A HSKFIAIGTS+GL+++FDHFQ IR VLG+      N A  D PVT++D+  GSD LV G+ SGR++LWD + +G  LK+V D H + VVS+R                           +P  ++ D  G+VNK+ F ++M   +VVD + L DG+AG I ++ VLP    +A  S                              + A+S+   TF++ I PEV+VI++W RP+         AP + + LP    +W     S P +      +LARGWGN +Q L    VV  GG                    S +     +W    VV  L +  ++                                                                        EWL                                                                       V G  P  ++                          AE ++DLL +Y+RLAI NAP +  +                      G +++L  SH+QMLAGVC E+CA+  R D+LFG+I+  F+   +  V ++ LEPY+L+ KL+ LSP  +  FV  F ++G     E+ +L L    LD+ T L L   H++YSAL+ +     D  ++  +VLLEA S   A   +P +E  A              A A          ++    PA  S  E                GYK+LLY+   F GR     EP+P  +  R RS +  HL  R
Sbjct:   94 LQIAEAREKQLLSCVGVELISPLQVKRRLRANARSKALKSRREGHIATKTNQRKMKVTEGTTAATTEKKSRGVVKVEPMEAISRQLRKNIEFKEYGPGSPTVVAIHSKFIAIGTSKGLVIIFDHFQNIRQVLGNI-----NDADGDGPVTAVDVSPGSDYLVCGYQSGRIVLWDMI-KGTSLKAVSDVHENPVVSLRFL----------------------KDQKPVLVSVDTNGLVNKLNFSKMMGMVYVVDVDPLYDGSAGKIKSISVLPQSAGNAKISYLT-----------------------DQYCLAALSTETVTFIIAIEPEVRVIYRWARPEDI-------APDDPV-LPSLAFAWI----SFPGSSRALAPVLARGWGNRVQFL---EVVFPGGKNHSHGRHGFPTFDEHDQIESSSAVMAIQWLGDQVVVYLNSHDEICVYDVMSRQELEIVDVSSLELVFASYRGNNARSFSNSFRGCYNILYLLGLKELQTARVLPWTQRIDLLVDDGEWLEALALALDHYEGLKVAAADRAARDRFPPVFFRDKQNDQCLVDILHMCQTNQRTGEKEDVFRHEESADEVRWVCGEVPYPADIAKKLEETLQKARSGEVTKNFVPISVAERVADLLMEYVRLAIANAPGSIAAAGGELSLNKI------------GMKLDLTKSHYQMLAGVCIEYCALINRTDLLFGEIYTRFKEANKLSVLVELLEPYILSEKLRNLSPVAMEEFVQHFSAQGKLAQVEQCLLHLNVAELDMDTILKLCHDHQLYSALIYIYNEGIDDFTTPIDVLLEACSNATASTNRPALESSARAFREPSGSRVSSLASAFGGTTRTTSSKVAIAKPAALSAREEAEEKVLGGSRRRRLFGYKLLLYISYAFSGRTFPKNEPIPLGKLGRVRSQICYHLFER 944