prot_P-fluviatile_contig5.11239.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: D8LRA1_ECTSI (tRNA(Ile)-lysidine synthetase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRA1_ECTSI)

HSP 1 Score: 760 bits (1962), Expect = 1.680e-274
Identity = 387/474 (81.65%), Postives = 404/474 (85.23%), Query Frame = 0

Query:    1 MALCLALVRLRPVYGFEVLGIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRR--------------GGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEYVGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPIPLDGGLVDVGPWRITTVEGEAIPPHELKGLAERGRPPAANTPPFRSMEELMRGELSYVVAVPDTEEAALTVQACLFPPGATARAPAWRDVDPRIRRGIPLVAPARSRDPVPLSESGARMVTVTLRYTG 460
            MALC ALV LRPVYGFEV+GIHIDYGNRHESGREADFV+GWC RHGVVF+KRAIGEVRRGVTAR+EYEKVAREIRFDAYKAAL  R              GGG        G     PAVMFGHH GDVEENVLTNLIKGCSILELAGMS ENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIP+MEDVFGSGVLGHLSAVARESDDLR LVHRELFQPVWDKAV QPLGLRL+VE YR+HGLLFWKEVLR+LVH MGMPMVRDKTLGMFVRDTLQ+D +VPLKDKWVELRKEYVG L  G LY+FR GVFLPPNTSYGEKAEGTP+PLDGGLV VGPWRITT E E   P EL+G  ERGRPPAAN  PF SMEELM GELSYVVAVPDT EAALTVQ     PG  A+APAWRDVDPRIRRG+PL+AP  SRDP  L ESGARMVTVTL+YTG
Sbjct:    1 MALCRALVHLRPVYGFEVVGIHIDYGNRHESGREADFVQGWCARHGVVFYKRAIGEVRRGVTARDEYEKVAREIRFDAYKAALGGRSKDGNSXXXXXXXXGGGKEEKGEGGGGR---PAVMFGHHEGDVEENVLTNLIKGCSILELAGMSPENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPMMEDVFGSGVLGHLSAVARESDDLRDLVHRELFQPVWDKAVTQPLGLRLDVESYRSHGLLFWKEVLRQLVHKMGMPMVRDKTLGMFVRDTLQRDTHVPLKDKWVELRKEYVGFLCGGSLYIFRMGVFLPPNTSYGEKAEGTPVPLDGGLVQVGPWRITTAETETKLPQELQGF-ERGRPPAANALPFASMEELMDGELSYVVAVPDTPEAALTVQ-----PGLRAKAPAWRDVDPRIRRGMPLLAPPPSRDPPLLKESGARMVTVTLKYTG 465          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: A0A6H5L0I6_9PHAE (tRNA(Ile)-lysidine synthetase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0I6_9PHAE)

HSP 1 Score: 762 bits (1967), Expect = 4.140e-273
Identity = 384/467 (82.23%), Postives = 403/467 (86.30%), Query Frame = 0

Query:    1 MALCLALVRLRPVYGFEVLGIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDE-------AACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEYVGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPIPLDGGLVDVGPWRITTVEGEAIPPHELKGLAERGRPPAANTPPFRSMEELMRGELSYVVAVPDTEEAALTVQACLFPPGATARAPAWRDVDPRIRRGIPLVAPARSRDPVPLSESGARMVTVTLRYTG 460
            MALC ALV L+PVYGFEV+GIHIDYGNRHESGREADFVEGWC+RHGVVF+KRAIGEVRRGVTAR+EYEKVAREIRFDAYKAAL  R           G +          PAVMFGHH GDVEENVLTNLIKGCSILELAGMS ENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIP+MEDVFGSGVLGHLSAVARESDDLR LVHRELFQPVWDKAV QPLGLRL+VE YR+HGLLFWKEVLR+LVH MGMPMVRDKTLGMFVRDTLQ+D +VPLKDKWVELRKEYVG L  G LY+FR GVFLPPNTSYGEKAEGTP+PLDGGLV VGPWRITT E E  PP EL+   ERGRPPAAN  PF SM+ELM GELSYVVAVPDT EAALTVQ     PG  A+APAWRDVDPRIRRG+PLVAP  SRDP  L ESGARMVTVTL+YTG
Sbjct:  140 MALCRALVHLQPVYGFEVMGIHIDYGNRHESGREADFVEGWCERHGVVFYKRAIGEVRRGVTARDEYEKVAREIRFDAYKAALGGRSNDGNGDDNEGGXKEEKGEGGGVRPAVMFGHHEGDVEENVLTNLIKGCSILELAGMSPENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPMMEDVFGSGVLGHLSAVARESDDLRDLVHRELFQPVWDKAVTQPLGLRLDVESYRSHGLLFWKEVLRQLVHKMGMPMVRDKTLGMFVRDTLQRDTHVPLKDKWVELRKEYVGFLCGGSLYIFRMGVFLPPNTSYGEKAEGTPVPLDGGLVQVGPWRITTAETETKPPQELQDF-ERGRPPAANALPFASMDELMDGELSYVVAVPDTPEAALTVQ-----PGLRAKAPAWRDVDPRIRRGMPLVAPPPSRDPPLLKESGARMVTVTLKYTG 600          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: A0A1Z5K323_FISSO (tRNA(Ile)-lysidine synthetase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K323_FISSO)

HSP 1 Score: 286 bits (731), Expect = 5.270e-85
Identity = 172/452 (38.05%), Postives = 250/452 (55.31%), Query Frame = 0

Query:    1 MALCLALVRLRPVYGFEVL-GIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEY-VGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPIPLDGGLVDVGPWRITT-VEGEAIPPHELKGLAERGRPPAANTPPFRSMEELMRGELSYVVAVPDTEEAALTVQACLFPPGATARAP-AWRDVDPRIRRGIPLVAPARSRDPVPLSESG 448
            M +   L +LR  +    L  +HIDY NR ES  EA +V+ +C + G+V+  R I EV RG+TAR+EYE++AREIR+ AY+ A+    G                 ++ GHH GD+ ENVL+N  KGC  L+L+GM++ +  +GV + RP+LP  K  ++D+AH+FGVPYFKDTTP WSTRGKLR +L+PL+E+V+G G + +LSA+A ESD+ R L H+   QP  D+   +P+GL+     ++  GL FWK VLR ++H+ G+ M  DK++  F+     K     LK+ W++ RK+Y V L   G + VF    F  P       A G  +   G    VGPW +T  V  + + P E   L E           +RS  E+M G + Y ++VP   +    V   LF        P AW+ +D +++  +PLV      D + L E G
Sbjct:  322 MVIAFVLNQLRAQFQLPPLVAVHIDYANRPESSAEAQYVQHYCDQLGIVYRCRRIDEVTRGITARDEYERIAREIRYQAYRDAIEEFDG---------------IGILLGHHKGDLRENVLSNAHKGCGPLDLSGMTAVSQNDGVTLLRPLLPLEKIAIFDYAHQFGVPYFKDTTPHWSTRGKLRNRLVPLLEEVYGEGSMNNLSALAVESDECRALFHQLALQPFLDQTTYKPMGLQFATAPWKEQGLFFWKFVLREVLHSAGLGMFSDKSVVSFLERVQAKT----LKEGWLQCRKDYGVHLQKDGTVSVFYPRCF--PFREADRFAVGEQLEY-GKAKTVGPWSVTAQVLPQELEPDESGTLVEMKA--------YRSFAEMMNGTIDYYISVPYIND---QVHPLLFTHFTRRTRPTAWKSLDLKVQEVLPLVG----NDGIALGELG 736          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: C1E3H1_MICCC (tRNA(Ile)-lysidine synthetase n=3 Tax=Micromonas TaxID=38832 RepID=C1E3H1_MICCC)

HSP 1 Score: 285 bits (728), Expect = 1.940e-84
Identity = 172/449 (38.31%), Postives = 245/449 (54.57%), Query Frame = 0

Query:    1 MALCLALVRLRPVYG-FEVLGIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPMVRDKTLGMFV-RDTLQKDVNVPLKDKWVELRKEYVGLLFQGDLYVFRSGVFLPPNTSYGEKAE-----GTPIPLDGGL-VDVGPWRITTVEGEAIPPHELKGLAERGRPPAANTPPFRSMEELMRGELSYVVAVPDTEEAALTVQACLFPPGA-----TARAPAWRDVDPRIRRGIPLVAPA 436
            M L   L    P +G F+V+ +HIDY NR ESG+EADFV GWC+R G+V   R I EV+RGVT RE+YE  +R IR+  YK   +  G                PAV  GHH GDV+EN++ N+ +G ++L + GM+ E  V GVR+WRPMLP  KD V DFAH FGVPYF DTTP+WSTRGKLR QL+PL+ D+FG G L +LS +  +S  L ++V   +  P   +  +   G   +        +LFWKE L+R+ H +G  M++++++   + R TL K      KD W+ L+K     +    L +F S  F  P+  + EK+      GT + LD      VGPW IT      + P+ ++G+A      A  +     +  ++R E++Y++ + ++  A          PGA      AR P  R VD ++ R +PLV PA
Sbjct:  309 MVLATILKAAAPSFGDFDVVAMHIDYANRPESGKEADFVRGWCERKGIVCVVRRIAEVKRGVTPREQYEAESRAIRYGLYKECAKTHG---------------FPAVFVGHHEGDVQENIIANIFRGANVLGMNGMNEEGVVEGVRIWRPMLPHSKDPVLDFAHTFGVPYFLDTTPTWSTRGKLRNQLLPLLADMFGDGFLRNLSLLGEDSAQLGRMVEDSVLGPFSKRMRLSDSGAYADFSGDEDKPMLFWKEALKRMCHGLGSGMMKERSVRELIDRFTLSK--RRCAKDGWITLKKLNKTFVTGKTLGMFSSEFF--PHYPWTEKSVWSEPVGTTVGLDAAEPTRVGPWTITA----RVVPNSIEGVA------ALESAAPLDVWAILRNEITYLLPLTESHAA----------PGAYYVDTKARIPPLRGVDAQVARALPLVVPA 718          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: A0A6B2KYV1_9EUKA (tRNA(Ile)-lysidine synthetase n=1 Tax=Arcella intermedia TaxID=1963864 RepID=A0A6B2KYV1_9EUKA)

HSP 1 Score: 281 bits (720), Expect = 4.190e-84
Identity = 149/350 (42.57%), Postives = 212/350 (60.57%), Query Frame = 0

Query:    1 MALCLALVRLRPVYGFEVLGIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGC-SILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEYVGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPI-PLDGG-LVDVGPWRIT 347
            M +   L+ L P + ++V+ IHIDY NR ESG EA +VE WCK+  V+F KR I EV RG+T RE YEK +R IRF+ Y++ L               D   C     GHH GD +EN++TN++KG  SIL L+GMS E+ + GV+VWRP +P  K V+YDF+HK+G+PYF DTTP WS RGK+R  L PL+ D+FG G L +LSA+  +S +L +L+   +F+PVW+      +G+   ++ +RT   LFWKE +R + ++MG+  VRDK++G F++   +       +  WV L+ E   L F+  L +FRS  F  P+TSY        + P D   +V  GPW +T
Sbjct:  220 MCILKCLLLLAPKFKYKVIAIHIDYANRPESGLEATYVEEWCKKFDVIFRKRVISEVTRGITDRELYEKESRRIRFEFYRSVLNE----------FQTDSGIC----LGHHKGDEQENIITNIMKGRESILNLSGMSPESIIEGVKVWRPCMPHPKSVIYDFSHKYGIPYFLDTTPVWSNRGKVRNHLQPLLGDIFGFGYLNNLSALGEDSKELSELLTATVFKPVWETIKRTEVGVWFNIKDHRTKPKLFWKETMRYIFYSMGVSTVRDKSMGHFMK---RMGEGFAKEGVWVSLKLENPALFFEDSLIIFRSSFF--PSTSYFTNKSTIEVSPTDPQTVVTFGPWTVT 550          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: A0A835YLZ2_9STRA (tRNA(Ile)-lysidine synthetase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLZ2_9STRA)

HSP 1 Score: 291 bits (745), Expect = 1.380e-83
Identity = 191/531 (35.97%), Postives = 267/531 (50.28%), Query Frame = 0

Query:    1 MALCLALVRLRPVYGFEVLGIHIDYGNRHESGREADFVEGW-----------------CKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALR---RRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPM--------------------------VRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEY----VGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPIPLDGGLVDVGPWRITTVEGEAIPPHELKGLAERGRPPAANTPPFRSMEELMRGELSYVVAVPDTEEAALTVQACLFPP------------GATARAPA-----WRDVDPRIRRGIPLVAPARSRDPVP----LSESGARMVTVTLRYTG 460
            M L   L  L+P YG+ ++ +HIDY NR ESG+EADFVE                   C    + F KR + EV RGVT R EYE V+RE   +A + A+    RR            + A CPA++FGHH GDV+ENV++N+++GCS+L++AGM  E+  +GV+VWRP+L F KD ++DFAH++ VPYFKDTTP WSTRGKLR  L+PL++D++G GVLGHLSA+A  SD+L   + RELFQP WD     P+G  +++  YR  GL FW+E L  L+H++G+PM                          V D+ L   +R   + +   P    W  LR+EY         +G LY+ +      P   Y   AEGT + +      V   ++   +  +            G  P    PPF  +   M G  S+ + +P +    L V     PP             A  RA A     +R +DP +R   PLV+ AR +  +      +  G R+V VT RY G
Sbjct: 1112 MVLAKILHHLQPAYGWRLVAVHIDYANRAESGKEADFVEQCVXXXXXXXXXXXTEPTSCHSLSITFRKRVVSEVTRGVTDRAEYEAVSREA-ANASRMAVTDEDRRDARYAAYAEVLREFAPCPAILFGHHRGDVQENVVSNVMRGCSLLKVAGMGEESINSGVKVWRPLLAFDKDAIFDFAHRYSVPYFKDTTPLWSTRGKLRNGLVPLLKDMYGDGVLGHLSALAAGSDELASELRRELFQPFWDSVERCPMGASVDLMPYRGKGLYFWREALMVLLHSLGLPMAXXXXXXXXXXXXXXXXXXXXXXXXXVSDRALITTLRRLGEPNAPPP-SAAWWPLRREYRCYCSEARIEGVLYILQPAA--APPEPYAVAAEGTEVEVSEPGPVVAADQLAAHKKGSRSXXXXXXXXXXGWLPELLPPPFADIRGFMSGAFSHTLPIPTSPPHDLRVAP---PPLLRERNAQRPDAAAQRRAEAPLPFPFRGMDPCLRACAPLVSCARGQAALEGWGDAAGGGVRVVRVTYRYVG 1635          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: A0A448ZEJ9_9STRA (tRNA(Ile)-lysidine synthetase n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448ZEJ9_9STRA)

HSP 1 Score: 278 bits (710), Expect = 3.320e-81
Identity = 169/453 (37.31%), Postives = 259/453 (57.17%), Query Frame = 0

Query:    1 MALCLALVRLRPVYGFEVLGI---HIDYGNRHESGREADFVEGWCKRH---GVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEYVGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPIPLDGGLVD------VGPWRITTVEGEAIPPHELKGLAERGRPPAA--NTPPFRSMEELMRGELSYVVAVP-----DTEEAALTVQACLFPPGATARAP-AWRDVDPRIRRGIPLV 433
            M +   L  L+   G++ L I   HIDY NR ESG EAD+V  +C+ H    + F  R IGEV RGVTAR++YE++AREIR+D+Y+ A+ R   G        G       V+ GHH GD+ ENVL+N  KGC  L+L+GM++ +  +G+ V+RP+LP  K +V+D+AH+FGVPY+KDTTP WSTRGKLR +L+PL+E+++G G + +LS +A ESD+ R L+++ +  P  D  V +P+G+ L+   ++   L FWK VLR  +H+ G+ M  DK++  F    L++      K  W++ RK+Y G+  Q D  V+   V  P +  + +K       +DG +V+      VG W++ +   E +    ++ L++   PPAA        SM+ LM G + Y V        D E      ++ +F   + A  P AW+  D RI+  +P++
Sbjct:  387 MVIASVLADLKRSRGYDHLAISAVHIDYANRPESGAEADYVRRYCEHHLGGAISFVCRRIGEVTRGVTARDDYERIAREIRYDSYREAVARAREGLGLLP---GGGPRTVGVVLGHHRGDLRENVLSNAHKGCGPLDLSGMTAVSRNDGITVFRPLLPLEKSLVFDYAHRFGVPYYKDTTPHWSTRGKLRNKLLPLLEEIYGDGSMNNLSNLAVESDECRALMNKSMIAPFLDSIVRRPMGIVLDTSAWKDQPLFFWKVVLREALHSAGLGMFSDKSVLEF----LKRIQATKPKAAWLQCRKDY-GVYLQEDGRVY---VLYPSSFPWSKKDA---YRVDGQVVEFGTDRTVGSWKVRS---EVLSDSAIESLSQ---PPAALLERRAVPSMDSLMDGNIEYFVKASTRFEKDKESGRFVPRSLVFRTFSKADRPRAWKSCDLRIQSTLPVL 819          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: A4RU81_OSTLU (tRNA(Ile)-lysidine synthetase n=2 Tax=Ostreococcus sp. 'lucimarinus' TaxID=242159 RepID=A4RU81_OSTLU)

HSP 1 Score: 274 bits (700), Expect = 6.320e-81
Identity = 153/431 (35.50%), Postives = 242/431 (56.15%), Query Frame = 0

Query:   15 GFEVLGIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHAMGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEYVGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPIPLD--GGLVDVGPWRI-------TTVEGEAIPPHELKGLAERGRPPAANTPPFRSMEELMRGELSYVVAVPDTEEAALTVQACLFPPGATARAPAWRDVDPRIRRGIPLVAPA 436
            G++V+ +HIDY NR ES  EADFV+ W  RHG+ F +  + ++RRGVT RE+YE  +R IR+  YK A  + G                PAV+ GHH GDV+EN++TNL++G ++L + GMS E  V GVR+WRPML   KD V  FAH +G+PYF D+TP+WSTRGKLR QL+PL+ED+FG G+L +LS +   S+ L ++V + LF+P WD      +G  ++ E + +  + FWK+V+R   H +G  M++D+++ + +   ++++ +   KD W+ L+KE    +  G+ +   +  F+P + +      GTPI +   G   D+G W I        +V+G   P   L+G             P  ++  ++  ++SY +   D   + +      FPP         +++D  +R  +PLV P+
Sbjct:  277 GYDVVTLHIDYKNRPESTEEADFVDDWSLRHGMKFERCTVDQIRRGVTPREQYEIESRRIRYGFYKEAGAKHG---------------FPAVLLGHHHGDVQENIITNLMRGANLLSVNGMSDEGVVEGVRIWRPMLSHVKDDVLTFAHAYGIPYFLDSTPTWSTRGKLRNQLVPLLEDMFGIGLLRNLSVIGENSEQLSEMVDKSLFKPFWDATKSSDVGCYVDCEPFISQPIFFWKQVIRETCHGLGASMMKDRSVRLLLA-RIKRERST--KDGWLCLKKENATFM-HGNTFGMFTTEFMPRSDTI---VPGTPIVVSSSGASFDIGNWHIDLEVVSNVSVDGNQCP---LEG-------------PAITVWNVLENDISYHIPYKDGTNSYVIDPEIRFPP--------TQNLDTAVRDALPLVVPS 661          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: R1DKB5_EMIHU (tRNA(Ile)-lysidine synthetase n=2 Tax=Emiliania huxleyi TaxID=2903 RepID=R1DKB5_EMIHU)

HSP 1 Score: 272 bits (696), Expect = 4.680e-80
Identity = 158/403 (39.21%), Postives = 227/403 (56.33%), Query Frame = 0

Query:    1 MALCLALVRLRPVYGFEVLGIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHA-MGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEYVGLLFQGDLYVFRSGVFLPPNTSYGEKAEGTPIP------LDGGLVDVGPWRITTV---EGEAIPPHELKGLAERGRPPAANTPPFRSMEELMRGELSYVVAV 393
            M L   L+RL+P +   V  +HIDY NR ESG EA F++ WC   GV    R + EVRRG T+R+EYEKV+R +RFDAY+ AL + GG                AV+FGHH+GDV ENV++N++KG S+L +AG+S+ + VNGV +WRPMLP  K ++YD+AH++GVPYFKD+TP WSTRG+LR QL PL+ +V+G G   HLSA+AR+S    +L+  +L+ P        PL +  +        L FW+E LR +    +G+ +V +K     V   L++     +++ W+ LRKE   LL  G L   R  VF       G    G  +P      + G  +  GPWR+      +G ++ P      ++  RP AA       + E++ G  +YV+ V
Sbjct:  312 MVLAHVLLRLQPRHRRRVCAVHIDYTNRAESGAEAAFLQRWCAARGVPLRVRTVEEVRRGATSRDEYEKVSRAVRFDAYRQALAQFGG---------------RAVLFGHHLGDVHENVISNVMKGASLLNIAGISTASAVNGVLIWRPMLPHPKSLLYDYAHRYGVPYFKDSTPGWSTRGRLRNQLQPLLAEVYGEGYSTHLSALARDSALCSELIDSQLYAPFRSSLRRSPLAVWFDAAGRAALPLFFWREALRHVCEKELGIGLVGEKA----VLQLLERLRAPRVREGWLALRKESRVLLAAGTLAFLRPSVFPGARRGRGLIGPGEAVPAPPADAVRGPTISFGPWRVRITRCAQGSSLGPGA--DTSDVARPAAA-----LGLWEVLSGRFAYVLPV 688          

BLAST of mRNA_P-fluviatile_contig5.11239.1 vs. uniprot
Match: A0A2D4C4Z2_PYTIN (tRNA(Ile)-lysidine synthetase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4C4Z2_PYTIN)

HSP 1 Score: 272 bits (696), Expect = 5.010e-79
Identity = 176/472 (37.29%), Postives = 250/472 (52.97%), Query Frame = 0

Query:    1 MALCLALVRLRPVYG-FEVLGIHIDYGNRHESGREADFVEGWCKRHGVVFFKRAIGEVRRGVTAREEYEKVAREIRFDAYKAALRRRGGGXXXXXXXXGDEAACPAVMFGHHMGDVEENVLTNLIKGCSILELAGMSSENTVNGVRVWRPMLPFGKDVVYDFAHKFGVPYFKDTTPSWSTRGKLRRQLIPLMEDVFGSGVLGHLSAVARESDDLRKLVHRELFQPVWDKAVVQPLGLRLEVEMYRTHGLLFWKEVLRRLVHA-MGMPMVRDKTLGMFVRDTLQKDVNVPLKDKWVELRKEYVGLLFQGD-LYVFRSGVFLPPNTSYGEKAEGTPIPLDGGLVDVGPWRI-TTVEGEAIPPHELKGLAERGRPPAANTPPFRSMEELMR-GELSYVVAVPDTEEAA--LTVQACLFPPGATARAPAWRDVDPRIRRG-------IPLVAPARSRDPVPLSESGARMVTVTLRY 458
            M +   L +LR  +  F ++ +HIDYGNR ESG E D+V  WC+R G++F  R + EV+R  T R++YE+++R+IR+  Y   + + G               CP + FGHH GDV+ENV++N++KG S+L L GMS  + VNGVR+WRP+L F KD +++FAH++GVPYFKDTTP WSTRGKLR  LIPL+ D++G G L +LS +  ES    +LV R +  P+ D      + + L+           WKEVLR + HA MG  MVR+K +   +   L +       D+W+ L+K     L     L +FR  VF P  T Y E+  GT + L G     GPW + T++E +   P  ++    RG           +M EL+  G++ Y  A+P + +AA  L V               W D  P +R         +PLV   R   P   S S  R V VT+ Y
Sbjct:  233 MVIAYLLHKLRSQHNNFAIVAVHIDYGNREESGAECDYVRRWCERFGIIFHARRVDEVKRATTKRDDYERISRDIRYGTYATVMAQYG---------------CPGMCFGHHRGDVQENVISNMMKGISLLNLNGMSESSVVNGVRIWRPLLAFDKDAIFEFAHRYGVPYFKDTTPRWSTRGKLRNHLIPLLRDMYGDGFLNNLSNLGAESMQCSELVDRTVLSPILDTVGSSEVAVWLDCTRLIGQPFFVWKEVLREVCHARMGNSMVREKPIRELMMK-LARHRGHETGDQWITLKKGNRSFLTADKVLIIFRDRVF-PLKTKYVER--GTAVTL-GASHTFGPWTVETSIETDTEHPRVVQW--RRGE--------VLTMSELVTTGQVQY--ALPCSADAAPELVVD--------------WEDRLPTLRMKEKVMTDFMPLVT-LRKAAP---SSSSERFVLVTIAY 654