prot_P-fluviatile_contig2.3667.1 (polypeptide) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: D8LCZ8_ECTSI (3-methyl-2-oxobutanoate hydroxymethyltransferase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LCZ8_ECTSI)

HSP 1 Score: 500 bits (1287), Expect = 7.830e-175
Identity = 254/319 (79.62%), Postives = 282/319 (88.40%), Query Frame = 0

Query:   86 DVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQHSPYKMPAGEEEAFHLLLAQDESRRNQVALEVKKLRERVDEDXXXXXGSNG---GPYG 401
            DV+KIRR+G+RK+TMVTA DYPS+VHVCRAGIDILLVGDS+GMVELGYDTTQPVTV DMLHHC+AVGRGARTP+VV D+PFGSYEVSP+EALRTAIRFVKEGNVDAVKLEGG+NR +HIRK+VDGGIAVMGHVGLTPQA+SVLGGFRAQGRTAVKAR+V++DALAVQEAGAFAVV+ECVPSPVAKAVTEAL+IPTIGIGAGP  +GQVLVYHDMLGMLQHP H QFVPRFCK+YAS+GEEVRRGLE FR+EVE GVFPQ +HSPYKMPAGEE+ F  LLA+DE  R +     KK  E  DE        +G   GPYG
Sbjct:    2 DVVKIRRRGERKVTMVTAYDYPSAVHVCRAGIDILLVGDSVGMVELGYDTTQPVTVGDMLHHCKAVGRGARTPLVVADMPFGSYEVSPKEALRTAIRFVKEGNVDAVKLEGGKNRAEHIRKVVDGGIAVMGHVGLTPQAISVLGGFRAQGRTAVKARQVVDDALAVQEAGAFAVVVECVPSPVAKAVTEALEIPTIGIGAGPCTSGQVLVYHDMLGMLQHPHHAQFVPRFCKNYASVGEEVRRGLENFRREVEGGVFPQEEHSPYKMPAGEEQQFKRLLAKDEHERLERLALKKKALEEQDEYETVSKDDDGDVEGPYG 320          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: A0A835Z145_9STRA (3-methyl-2-oxobutanoate hydroxymethyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z145_9STRA)

HSP 1 Score: 390 bits (1003), Expect = 4.360e-132
Identity = 199/315 (63.17%), Postives = 240/315 (76.19%), Query Frame = 0

Query:   87 VMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQHSPYKMPAGEEEAFHLLLAQDESRRNQVA-LEVKKLRERVDEDXXXXXGSNGGPY 400
            V+K+ R+ +RKITMVTA DYPSSVHVC AGIDI LVGDS+GMV LGY+TTQPVTV++MLHHCRAV RGA  P++VGD+PFGSYEVSPE+AL+TA RFVKEG VDAVKLEGGR R +HI++IV+GG+AVMGHVGLTPQA+SVLGGFRAQGR+ +            QEAGAFAVV+ECVP+ V +A+ +AL+IP IGIGAGP   GQVLVYHDMLG+L HP H +  P+FCK +A +  EV +GL A+R+EVE G FP A HSPY M   EE  F  LLA D   R + A L  KKL+E+ + +     G  GG +
Sbjct:    3 VLKLAREKKRKITMVTAYDYPSSVHVCEAGIDIALVGDSVGMVTLGYETTQPVTVEEMLHHCRAVKRGAAGPLLVGDMPFGSYEVSPEQALQTAYRFVKEGGVDAVKLEGGRTRTEHIKRIVEGGVAVMGHVGLTPQAISVLGGFRAQGRSVI------------QEAGAFAVVLECVPAVVGRAIADALEIPVIGIGAGPHTDGQVLVYHDMLGILSHPHHQRMAPKFCKVFADVQTEVMKGLHAYRREVEAGAFPGAAHSPYAMTPAEEANFEHLLAHDARERREAAELTKKKLKEQDELEAVRLYGQGGGGF 305          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: W7TQW2_9STRA (3-methyl-2-oxobutanoate hydroxymethyltransferase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TQW2_9STRA)

HSP 1 Score: 390 bits (1003), Expect = 3.440e-131
Identity = 198/305 (64.92%), Postives = 243/305 (79.67%), Query Frame = 0

Query:   80 KRTSVTDVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQHSPYKMPAGEEEAFHLLLAQDE-SRRNQVALEVKKLRE 383
            K+ SV D++  +R+ QR I+MVTA DYPS++HV  AGIDI+L GDS+ MVELGYDTTQPV ++ MLHHC+AV RG +  ++VGD+PFGSYEVS E A+  A RF+KEG  DAVK+EGGR+R   + KIV  GIAVMGHVGLTPQA+SVLGGFRAQGRTAVKAR +++DALA+QEAGAFAVV+ECVP+PVA+A+TEAL+IPTIGIGAGP   GQVLV+HDMLGM  HP H  FVPRFCK YA +G  +R GLE+F+Q+VE G FP   +SPYKMPA E  AF  L+AQD  SR+ Q     ++L++
Sbjct:   60 KKMSVLDLLHKKRKNQR-ISMVTAYDYPSALHVDLAGIDIVLCGDSVAMVELGYDTTQPVDMEQMLHHCKAVARGTKRSLIVGDMPFGSYEVSEELAMTNAFRFIKEGFTDAVKIEGGRDRAHIVEKIVKNGIAVMGHVGLTPQAISVLGGFRAQGRTAVKARGLVDDALALQEAGAFAVVVECVPAPVAQAITEALEIPTIGIGAGPHTCGQVLVFHDMLGMTTHPHHEAFVPRFCKRYAQLGNVIREGLESFKQDVEAGTFPSEAYSPYKMPAAELRAFQALMAQDATSRQTQAHRAAERLKQ 363          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: A0A4D9CXI1_9STRA (3-methyl-2-oxobutanoate hydroxymethyltransferase n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CXI1_9STRA)

HSP 1 Score: 382 bits (981), Expect = 5.040e-129
Identity = 191/285 (67.02%), Postives = 229/285 (80.35%), Query Frame = 0

Query:  100 MVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQHSPYKMPAGEEEAFHLLLAQDE-SRRNQVALEVKKLRE 383
            MVTA DYPS++HV  AGIDI+L GDS+ MVELGYDTTQPV ++ MLHHC+AV RG +  ++VGD+PFGSYEVS E A+  A RF+KEG  DAVK+EGGR+R   + KIV  GIAVMGHVGLTPQA+SVLGGFRAQGRTAVKAR +++DALA+QEAGAFAVV+ECVP+PVA+A+TEAL+IPTIGIGAGP   GQVLVYHDMLGM  HP H  FVPRFCK YA +G  +R GLE+F+Q+VE G FP   +SPYKMPA E  AF  L+AQD  SR+ Q     ++L++
Sbjct:    1 MVTAYDYPSALHVDLAGIDIVLCGDSVAMVELGYDTTQPVDMEQMLHHCKAVARGTKRSLIVGDMPFGSYEVSEELAMTNAFRFIKEGFTDAVKIEGGRDRAHIVEKIVKNGIAVMGHVGLTPQAISVLGGFRAQGRTAVKARGLVDDALALQEAGAFAVVVECVPAPVAQAITEALEIPTIGIGAGPHTCGQVLVYHDMLGMTTHPHHEAFVPRFCKRYAQLGNVIREGLESFKQDVEAGTFPSEAYSPYKMPAAELRAFQALMAQDATSRQTQAHRAAERLKQ 285          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: A0A7S2FBI3_9STRA (3-methyl-2-oxobutanoate hydroxymethyltransferase n=2 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2FBI3_9STRA)

HSP 1 Score: 372 bits (956), Expect = 5.240e-125
Identity = 186/302 (61.59%), Postives = 237/302 (78.48%), Query Frame = 0

Query:   86 DVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQHSPYKMPAGEEEAFHLLLAQDESRRNQVALEVKKLRERVDE 387
            +++ ++R+G+R I+MVTA +YPS+V V  A ID++LVGDS+GMVELGY+TTQPVT++DMLHH +AV RG    ++V DLPFGSYE+SP +AL++A R VKEG  DAVKLEGGR+R D  R IV GGIAVMGH+GLTPQA+SV+GGFRAQGRT VKAR +L+DALA+Q+AG FA+V+ECVP  VA+ +TE+L+IPTIGIGAGP  TGQVLVYHDMLGML HP H QFVP+FCK YA +G  +R GL+AF+ +V++G+FP  + +PYKM   EE  F  L+A+DE  R        K  +  DE
Sbjct:    1 ELLAMKRRGKR-ISMVTAYNYPSAVQVDLANIDVVLVGDSVGMVELGYETTQPVTLEDMLHHAKAVKRGVERSLIVVDLPFGSYEISPAQALQSAYRCVKEGGADAVKLEGGRDRADTCRSIVKGGIAVMGHIGLTPQAISVIGGFRAQGRTGVKARALLDDALALQDAGVFALVLECVPPQVAQVITESLEIPTIGIGAGPHTTGQVLVYHDMLGMLSHPHHEQFVPKFCKKYAEVGHAIRFGLDAFKSDVDQGLFPADEFTPYKMSDKEEIKFRELVAEDEGVRQSKLTRASKRLKDADE 301          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: A0A7S2XAE6_9EUKA (3-methyl-2-oxobutanoate hydroxymethyltransferase (Fragment) n=2 Tax=Lotharella TaxID=74819 RepID=A0A7S2XAE6_9EUKA)

HSP 1 Score: 371 bits (952), Expect = 1.480e-123
Identity = 182/287 (63.41%), Postives = 230/287 (80.14%), Query Frame = 0

Query:   79 RKRTSVTDVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQ-HSPYKMPAGEEEAFHLLL 364
            R++ S  +++  RR+G+R ITMVTA DYPS++H   AGID++LVGDS GMVELGYDTTQPVT+D+MLHH +AV RGA+ P++VGDLPFG+YEVSPE+A RTA RFVKE  VD VK+EGGR       K+V+GG+AVMGH+GLTPQ +SVLGGFRAQGRTA+KAR +++DALA+++AG FA+VIECVP  V +A+TEAL+IPTIGIG+GP  TGQVLVYHDMLGM  HP +  F P+FCK +  +GE +R+GLE F++EVE G FP  Q HSPYKM   E+  F  ++
Sbjct:   49 RRKMSAMELIAKRRRGER-ITMVTAYDYPSALHCDLAGIDVVLVGDSCGMVELGYDTTQPVTIDEMLHHAKAVVRGAKHPLIVGDLPFGTYEVSPEQAQRTAYRFVKEAGVDCVKMEGGRKMAPFASKVVNGGVAVMGHIGLTPQHISVLGGFRAQGRTAIKARELVDDALALEDAGCFAIVIECVPPQVGEAITEALEIPTIGIGSGPGTTGQVLVYHDMLGMTVHPHYKAFTPKFCKKFVDLGETIRQGLEGFKREVESGSFPSLQDHSPYKMSNKEQAMFEEMM 334          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: A0A7S3YF20_9EUKA (3-methyl-2-oxobutanoate hydroxymethyltransferase n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S3YF20_9EUKA)

HSP 1 Score: 370 bits (949), Expect = 2.810e-123
Identity = 181/275 (65.82%), Postives = 226/275 (82.18%), Query Frame = 0

Query:   79 RKRTSVTDVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQ-HSPYKM 352
            R++ S  +++  RR+G+R ITMVTA DYPS++H   AGIDI+LVGDS GMVELGYDTTQPVT+D+M+HH RAV RGA+ P+VVGDLPFGSYEV+PE+A +TA RFVKE  VD VK+EGGR      +K+V+GG+AVMGHVGLTPQA+SVLGGFRAQGRTA+KAR +++DALA+++AG FAVV+ECVP  V +A+T+AL+IPTIGIG+GP  TGQVLVYHDMLGM  HP +  F P+FCK +  +GE +R+GLE F++EVE G FP    HSPYKM
Sbjct:   54 RRKMSAMELIAKRRRGER-ITMVTAYDYPSALHCDLAGIDIVLVGDSCGMVELGYDTTQPVTIDEMMHHARAVVRGAKHPLVVGDLPFGSYEVNPEQAQQTAYRFVKEAGVDCVKMEGGRKMAPFAQKVVNGGVAVMGHVGLTPQAISVLGGFRAQGRTAIKARELVDDALALEDAGCFAVVVECVPPQVGQAITQALEIPTIGIGSGPGTTGQVLVYHDMLGMTVHPHYKAFTPKFCKKFVDLGETIRQGLEGFKKEVESGTFPSLDSHSPYKM 327          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: K3W4X2_GLOUD (3-methyl-2-oxobutanoate hydroxymethyltransferase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3W4X2_GLOUD)

HSP 1 Score: 362 bits (929), Expect = 4.050e-120
Identity = 183/286 (63.99%), Postives = 225/286 (78.67%), Query Frame = 0

Query:   79 RKRTSVTDVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQHSPYKMPAGEEEAFHLLL 364
            R++ +  D+  ++R+ ++ ITMVTA DYPS+VHV  AG DILL+GDSLGMVELG DTT PVT+DDM+HH +AV RGA  P+++ D+PFGS E  P EAL+ A R +KEG  D VK+EGGR R   I+ IVDGGIAVMGHVGL PQ +SVLGGFRAQGRTAV+A +++EDALAVQEAGAFAVV+ECVPSPVAK VTE L +PTIGIGAGP  +GQVLV+HD+LGMLQHP H Q VP+FCK YA +GE +R GLE +R++VE   FP  +++PYKM A E E    L+
Sbjct:   55 RRKVTTLDLGILKRK-KKPITMVTAYDYPSAVHVDLAGFDILLIGDSLGMVELGMDTTIPVTMDDMIHHTQAVKRGAERPLIIADMPFGSCEGDPYEALKNAQRLLKEGGADCVKIEGGRERAKTIKLIVDGGIAVMGHVGLRPQHISVLGGFRAQGRTAVQAEQIIEDALAVQEAGAFAVVLECVPSPVAKQVTELLRVPTIGIGAGPDTSGQVLVFHDLLGMLQHPHHAQHVPKFCKRYADVGERIRIGLEEYREDVENRRFPSEEYAPYKMSAEETEKLQELV 339          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: A0A7S0H7K8_9EUKA (3-methyl-2-oxobutanoate hydroxymethyltransferase (Fragment) n=1 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0H7K8_9EUKA)

HSP 1 Score: 361 bits (926), Expect = 6.280e-120
Identity = 180/291 (61.86%), Postives = 230/291 (79.04%), Query Frame = 0

Query:   79 RKRTSVTDVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQ-AQHSPYKMPAGEEEAFHLLLAQDE 368
            R++ S  +++  RR+G+ KITMVTA DYPS++H   AGIDI+LVGDSLGMVELGY+ T PVT +++LHH +AV RGA+ P+++ DLPFGSYEV P++A  +A +FVKE  VDAVK+EGGR     ++K+VDGGIAVMGHVGLTPQ ++VLGGFRAQGRTA KAR +L+DALA++ AGAFA+VIECVP+ V +A+T AL+IPTIGIGAGP  +GQVLVYHDMLGM  HP +  F P+FCK +  +GE +R GLE F++EVE G FP  + HSPYKM   E+E F  +L+ DE
Sbjct:   56 RRKMSALELISRRRKGE-KITMVTAYDYPSALHCDLAGIDIILVGDSLGMVELGYENTLPVTPEELLHHAKAVVRGAKHPLIIADLPFGSYEVDPKQAQESAYKFVKEAGVDAVKMEGGRRLAPTVKKVVDGGIAVMGHVGLTPQHINVLGGFRAQGRTAKKARDLLDDALALEAAGAFAIVIECVPAQVGEAITNALEIPTIGIGAGPGTSGQVLVYHDMLGMTVHPHYKAFTPKFCKKFVDLGETIREGLECFKKEVETGEFPSFSDHSPYKMSKKEQELFREMLSVDE 345          

BLAST of mRNA_P-fluviatile_contig2.3667.1 vs. uniprot
Match: A0A024GNC3_9STRA (3-methyl-2-oxobutanoate hydroxymethyltransferase n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024GNC3_9STRA)

HSP 1 Score: 357 bits (916), Expect = 7.650e-119
Identity = 175/280 (62.50%), Postives = 222/280 (79.29%), Query Frame = 0

Query:   79 RKRTSVTDVMKIRRQGQRKITMVTACDYPSSVHVCRAGIDILLVGDSLGMVELGYDTTQPVTVDDMLHHCRAVGRGARTPMVVGDLPFGSYEVSPEEALRTAIRFVKEGNVDAVKLEGGRNRVDHIRKIVDGGIAVMGHVGLTPQAVSVLGGFRAQGRTAVKARRVLEDALAVQEAGAFAVVIECVPSPVAKAVTEALDIPTIGIGAGPFATGQVLVYHDMLGMLQHPDHGQFVPRFCKSYASIGEEVRRGLEAFRQEVEEGVFPQAQHSPYKMPAGEEE 358
            RK+ + TD+  ++R+G + ITM+TA DYPS+VHV  AG D+LLVGDSLGMVELG  TT PVT+DDM+HHC+AV RGA  P+++ DLPFG+ E SP EAL  A R +KEG  D VK+EGG+ R   IR IVD GIAV+GHVGL PQ +SV+GGFRAQGRTA +A +++EDA+A+++AGAFA+VIECVPS VA+ +T+ L+IPTIGIGAGP   GQVLV+HD+LGMLQHP H QFVP+FCK YA +G+ +R+GLE +R +VE   FP  Q++PYKM   EEE
Sbjct:    3 RKKVTATDLRVLKRRG-KPITMITAYDYPSAVHVDLAGFDVLLVGDSLGMVELGMSTTLPVTLDDMIHHCKAVKRGATRPLILVDLPFGTCEGSPFEALSNAQRLLKEGGADCVKIEGGQERAKTIRTIVDAGIAVVGHVGLRPQHISVMGGFRAQGRTAEQAEKIIEDAIAIEKAGAFALVIECVPSTVARHITDILEIPTIGIGAGPHTCGQVLVFHDLLGMLQHPHHAQFVPKFCKRYADVGQAIRKGLENYRDDVENHTFPSEQYTPYKMTQEEEE 281