mRNA_P-fluviatile_contig89.14998.1 (mRNA) Porterinema fluviatile SAG_2381

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-fluviatile_contig89.14998.1
Unique NamemRNA_P-fluviatile_contig89.14998.1
TypemRNA
OrganismPorterinema fluviatile SAG_2381 (Porterinema fluviatile SAG_2381)
Homology
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: D1J7A7_ECTSI (Light-independent protochlorophyllide reductase subunit N n=42 Tax=Phaeophyceae TaxID=2870 RepID=D1J7A7_ECTSI)

HSP 1 Score: 270 bits (689), Expect = 4.370e-87
Identity = 129/136 (94.85%), Postives = 134/136 (98.53%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSV +I+PKGLKEREEE+W KLKYYISLI+GKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKN+PIPIIVEKPDNFNQVDRIR
Sbjct:  250 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVFEIQPKGLKEREEEIWGKLKYYISLIDGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNVPIPIIVEKPDNFNQVDRIR 385
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A8F0JZA7_9PHAE (Light-independent protochlorophyllide reductase subunit N n=1 Tax=Chorda asiatica TaxID=1281577 RepID=A0A8F0JZA7_9PHAE)

HSP 1 Score: 263 bits (673), Expect = 1.010e-84
Identity = 125/136 (91.91%), Postives = 133/136 (97.79%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKIC V  +EPKGL++RE+EVWEKLKYYISLI+GKSVFFMGDNLLEISLARFLI+SGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPII+EKPDN+NQVDRIR
Sbjct:  246 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICMVFGVEPKGLQKREDEVWEKLKYYISLIDGKSVFFMGDNLLEISLARFLIKSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIIEKPDNYNQVDRIR 381
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A8F0F745_9PHAE (Light-independent protochlorophyllide reductase subunit N n=1 Tax=Protohalopteris sp. TaxID=2843287 RepID=A0A8F0F745_9PHAE)

HSP 1 Score: 259 bits (663), Expect = 2.740e-83
Identity = 123/136 (90.44%), Postives = 133/136 (97.79%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSV  I+P GLKEREEE+W+KL+YYISLINGKSVFFMGDNLLEISLARFLI+SGMIVFEIGIPYMDKRYQGAELQLLQ+TCKE++IPIPII+EKPDN+NQVDRIR
Sbjct:  240 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVFCIQPTGLKEREEEIWKKLEYYISLINGKSVFFMGDNLLEISLARFLIKSGMIVFEIGIPYMDKRYQGAELQLLQQTCKEQHIPIPIIIEKPDNYNQVDRIR 375
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A8E5XRE7_9PHAE (Light-independent protochlorophyllide reductase subunit N n=1 Tax=Ishige okamurae TaxID=233772 RepID=A0A8E5XRE7_9PHAE)

HSP 1 Score: 251 bits (640), Expect = 8.190e-80
Identity = 116/136 (85.29%), Postives = 129/136 (94.85%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRR+KCKLIGAPFPIGPDGTRAWLEKIC+V  +EP GLKEREEE+WEKLK+Y+SL+ GKSVFFMGDNLLEISLARFLIRSGM+V+EIGIPYMDKRYQ AELQLL+KTCKE+  PIPII+EKPDN+NQVDRIR
Sbjct:  241 ATTLMRRKKCKLIGAPFPIGPDGTRAWLEKICAVFGVEPSGLKEREEEIWEKLKFYLSLVKGKSVFFMGDNLLEISLARFLIRSGMMVYEIGIPYMDKRYQAAELQLLEKTCKEQQKPIPIIIEKPDNYNQVDRIR 376
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A141BSG8_9PHAE (Light-independent protochlorophyllide reductase subunit N n=14 Tax=Sargassaceae TaxID=3014 RepID=A0A141BSG8_9PHAE)

HSP 1 Score: 249 bits (636), Expect = 3.750e-79
Identity = 114/136 (83.82%), Postives = 129/136 (94.85%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKIC++L+I+P GLKERE+E+W K+  YISLI GKSVFFMGDNLLEISLARFL+R+GM +FEIGIPYMDKRYQGAEL+LLQKTCKEK +P+PII+EKPDN+NQVDRIR
Sbjct:  246 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICTILKIQPIGLKEREDEIWNKMSNYISLIKGKSVFFMGDNLLEISLARFLLRAGMTIFEIGIPYMDKRYQGAELELLQKTCKEKKVPLPIIIEKPDNYNQVDRIR 381
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A2R4QPZ1_9PHAE (Light-independent protochlorophyllide reductase subunit N n=3 Tax=Fucaceae TaxID=3010 RepID=A0A2R4QPZ1_9PHAE)

HSP 1 Score: 246 bits (629), Expect = 4.470e-78
Identity = 114/136 (83.82%), Postives = 129/136 (94.85%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRRRKCKLIGAPFPIGPDGTRAW+ KIC+V  I+P+GL+ERE E+W+K+K Y++LINGKSVFFMGDNLLEISLARFLIR+GMIVFEIGIPYMDKRYQGAEL LLQKTCK KN+P+PII+EKPDN+NQVDRIR
Sbjct:  248 ATTLMRRRKCKLIGAPFPIGPDGTRAWITKICTVFNIQPQGLEEREIEIWKKMKNYLNLINGKSVFFMGDNLLEISLARFLIRAGMIVFEIGIPYMDKRYQGAELDLLQKTCKIKNLPLPIIIEKPDNYNQVDRIR 383
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A7S6U9V0_9STRA (Light-independent protochlorophyllide reductase subunit N n=1 Tax=Schizocladia ischiensis TaxID=196139 RepID=A0A7S6U9V0_9STRA)

HSP 1 Score: 246 bits (627), Expect = 7.810e-78
Identity = 113/136 (83.09%), Postives = 129/136 (94.85%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRR+KCKLIGAPFPIGPDGTRAWLEKIC V  I+P+GL++RE E+W+KL +Y+ LI+GKSVFFMGDNLLEISLARFLIR+GMIV+EIGIPYMDKRYQGAEL LL+KTCKEKNIP+PIIVEKPDN+NQVDRI+
Sbjct:  243 ATTLMRRKKCKLIGAPFPIGPDGTRAWLEKICYVFNIKPEGLEKRENEIWQKLDFYLKLIDGKSVFFMGDNLLEISLARFLIRAGMIVYEIGIPYMDKRYQGAELNLLEKTCKEKNIPVPIIVEKPDNYNQVDRIQ 378
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A2I4Q2D6_9PHAE (Light-independent protochlorophyllide reductase subunit N n=1 Tax=Dictyopteris divaricata TaxID=156996 RepID=A0A2I4Q2D6_9PHAE)

HSP 1 Score: 244 bits (624), Expect = 2.590e-77
Identity = 114/136 (83.82%), Postives = 127/136 (93.38%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRR+KCKLIGAPFPIGPDGTRAW+EKIC VL IEP+GL ERE E+W+ L+ YI+LI  KSVFFMGDNLLEISLARFLI++GM+VFEIGIPYMDKRYQGAELQLLQ+TCKEK IPIPII+EKPDN+NQVDRIR
Sbjct:  249 ATTLMRRKKCKLIGAPFPIGPDGTRAWIEKICKVLNIEPQGLDERENEIWKNLQDYINLIENKSVFFMGDNLLEISLARFLIKAGMVVFEIGIPYMDKRYQGAELQLLQQTCKEKKIPIPIIIEKPDNYNQVDRIR 384
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: A0A7G5XUZ2_9MARC (Light-independent protochlorophyllide reductase subunit N n=10 Tax=Marchantiophyta TaxID=3195 RepID=A0A7G5XUZ2_9MARC)

HSP 1 Score: 233 bits (595), Expect = 8.200e-73
Identity = 108/136 (79.41%), Postives = 124/136 (91.18%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR 409
            ATTLMRRRKCKLIGAPFPIGPDGTRAW+EKICSV  IEP+GL+ERE+++WE LK Y++L+ GKSVFFMGDNLLEISLARFLIR GMIV+EIGIPYMDKRYQ AEL LLQ+TCK+  IP+P IVEKPDN+NQ+ R+R
Sbjct:  260 ATTLMRRRKCKLIGAPFPIGPDGTRAWIEKICSVFNIEPQGLEEREQQIWESLKNYLNLVRGKSVFFMGDNLLEISLARFLIRCGMIVYEIGIPYMDKRYQAAELTLLQETCKKMCIPMPRIVEKPDNYNQIQRMR 395
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BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Match: UPI00145F520E (ferredoxin:protochlorophyllide reductase (ATP-dependent) subunit N n=1 Tax=Brasilonema bromeliae TaxID=383615 RepID=UPI00145F520E)

HSP 1 Score: 226 bits (577), Expect = 3.030e-72
Identity = 107/135 (79.26%), Postives = 118/135 (87.41%), Query Frame = 2 
Query:    2 ATTLMRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLKYYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAELQLLQKTCKEKNIPIPIIVEKPDNFNQVDRI 406
            ATTLMRRRKCKLIGAPFPIGPDGTRAW+EKICSV  I PKGL ERE ++WE L+ YI LI GKSVFFMGDNLLE+SLARFL+R GM V EIGIPYMDKRYQ AEL+LL+KTCKE  +P+P IVEKPDN+NQV RI
Sbjct:   83 ATTLMRRRKCKLIGAPFPIGPDGTRAWIEKICSVFGITPKGLDEREAQIWESLEDYIKLIRGKSVFFMGDNLLEVSLARFLVRCGMTVPEIGIPYMDKRYQDAELKLLEKTCKEMGVPLPRIVEKPDNYNQVQRI 217
The following BLAST results are available for this feature:
BLAST of mRNA_P-fluviatile_contig89.14998.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D1J7A7_ECTSI4.370e-8794.85Light-independent protochlorophyllide reductase su... [more]
A0A8F0JZA7_9PHAE1.010e-8491.91Light-independent protochlorophyllide reductase su... [more]
A0A8F0F745_9PHAE2.740e-8390.44Light-independent protochlorophyllide reductase su... [more]
A0A8E5XRE7_9PHAE8.190e-8085.29Light-independent protochlorophyllide reductase su... [more]
A0A141BSG8_9PHAE3.750e-7983.82Light-independent protochlorophyllide reductase su... [more]
A0A2R4QPZ1_9PHAE4.470e-7883.82Light-independent protochlorophyllide reductase su... [more]
A0A7S6U9V0_9STRA7.810e-7883.09Light-independent protochlorophyllide reductase su... [more]
A0A2I4Q2D6_9PHAE2.590e-7783.82Light-independent protochlorophyllide reductase su... [more]
A0A7G5XUZ2_9MARC8.200e-7379.41Light-independent protochlorophyllide reductase su... [more]
UPI00145F520E3.030e-7279.26ferredoxin:protochlorophyllide reductase (ATP-depe... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-fluviatile_contig89contigP-fluviatile_contig89:147224..147632 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Porterinema fluviatile SAG_23812021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score264.6
Seed ortholog evalue2.4e-68
Seed eggNOG ortholog2880.D1J7A7
Preferred namechlN
KEGG rclassRC01008
KEGG koko:K04038
KEGG ReactionR06282
KEGG Pathwayko00860,ko01100,ko01110,map00860,map01100,map01110
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0009507,GO:0009536,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044424,GO:0044444,GO:0044464
EggNOG free text desc.Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (ChlN-ChlB) is the catalytic component of the complex
EggNOG OGs2QQ7U@2759,COG2710@1
EC1.3.7.7
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categoryother localisation
Exons1
Model size410
Cds size396
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817301.2606802-UTR-P-fluviatile_contig89:147223..1472361622817301.2606802-UTR-P-fluviatile_contig89:147223..147236Porterinema fluviatile SAG_2381UTRP-fluviatile_contig89 147224..147236 +
1693571994.9308596-UTR-P-fluviatile_contig89:147223..1472361693571994.9308596-UTR-P-fluviatile_contig89:147223..147236Porterinema fluviatile SAG_2381UTRP-fluviatile_contig89 147224..147236 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817301.2727613-CDS-P-fluviatile_contig89:147236..1476321622817301.2727613-CDS-P-fluviatile_contig89:147236..147632Porterinema fluviatile SAG_2381CDSP-fluviatile_contig89 147237..147632 +
1693571994.9461565-CDS-P-fluviatile_contig89:147236..1476321693571994.9461565-CDS-P-fluviatile_contig89:147236..147632Porterinema fluviatile SAG_2381CDSP-fluviatile_contig89 147237..147632 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-fluviatile_contig89.14998.1prot_P-fluviatile_contig89.14998.1Porterinema fluviatile SAG_2381polypeptideP-fluviatile_contig89 147237..147632 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-fluviatile_contig89.14998.1

>prot_P-fluviatile_contig89.14998.1 ID=prot_P-fluviatile_contig89.14998.1|Name=mRNA_P-fluviatile_contig89.14998.1|organism=Porterinema fluviatile SAG_2381|type=polypeptide|length=132bp
MRRRKCKLIGAPFPIGPDGTRAWLEKICSVLQIEPKGLKEREEEVWEKLK
YYISLINGKSVFFMGDNLLEISLARFLIRSGMIVFEIGIPYMDKRYQGAE
LQLLQKTCKEKNIPIPIIVEKPDNFNQVDRIR
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mRNA from alignment at P-fluviatile_contig89:147224..147632+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-fluviatile_contig89.14998.1 ID=mRNA_P-fluviatile_contig89.14998.1|Name=mRNA_P-fluviatile_contig89.14998.1|organism=Porterinema fluviatile SAG_2381|type=mRNA|length=409bp|location=Sequence derived from alignment at P-fluviatile_contig89:147224..147632+ (Porterinema fluviatile SAG_2381)
TGCAACTACATTAATGAGAAGAAGAAAATGTAAGCTTATTGGGGCACCAT
TTCCTATTGGTCCCGATGGAACAAGAGCTTGGTTAGAAAAAATTTGTAGT
GTTTTGCAGATCGAACCTAAAGGATTAAAAGAGCGAGAGGAAGAAGTCTG
GGAGAAATTAAAGTATTATATTAGTTTAATTAACGGGAAAAGTGTTTTTT
TCATGGGTGACAATTTACTAGAAATTTCACTAGCACGTTTTTTAATACGT
TCAGGAATGATTGTTTTTGAGATCGGTATACCGTATATGGATAAACGATA
TCAAGGTGCTGAGTTACAATTACTACAAAAAACTTGTAAAGAAAAAAATA
TTCCAATTCCTATTATTGTTGAGAAACCAGATAATTTTAATCAAGTAGAT
AGAATACGT
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Coding sequence (CDS) from alignment at P-fluviatile_contig89:147224..147632+

>mRNA_P-fluviatile_contig89.14998.1 ID=mRNA_P-fluviatile_contig89.14998.1|Name=mRNA_P-fluviatile_contig89.14998.1|organism=Porterinema fluviatile SAG_2381|type=CDS|length=792bp|location=Sequence derived from alignment at P-fluviatile_contig89:147224..147632+ (Porterinema fluviatile SAG_2381)
ATGAGAAGAAGAAAATGTAAGCTTATTGGGGCACCATTTCCTATTGGTCC
CGATGGAACAAGAGCTTGGTTAGAAAAAATTTGTAGTGTTTTGCAGATCG
AACCTAAAGGATTAAAAGAGCGAGAGGAAGAAGTCTGGGAGAAATTAAAG
TATTATATTAGTTTAATTAACGGGAAAAGTGTTTTTTTCATGGGTGACAA
TTTACTAGAAATTTCACTAGCACGTTTTTTAATACGTTCAGGAATGATTG
TTTTTGAGATCGGTATACCGTATATGGATAAACGATATCAAGGTGCTGAG
TTACAATTACTACAAAAAACTTGTAAAGAAAAAAATATTCCAATTCCTAT
TATTGTTGAGAAACCAGATAATTTTAATCAAGTAGATAGAATACGTATGA
GAAGAAGAAAATGTAAGCTTATTGGGGCACCATTTCCTATTGGTCCCGAT
GGAACAAGAGCTTGGTTAGAAAAAATTTGTAGTGTTTTGCAGATCGAACC
TAAAGGATTAAAAGAGCGAGAGGAAGAAGTCTGGGAGAAATTAAAGTATT
ATATTAGTTTAATTAACGGGAAAAGTGTTTTTTTCATGGGTGACAATTTA
CTAGAAATTTCACTAGCACGTTTTTTAATACGTTCAGGAATGATTGTTTT
TGAGATCGGTATACCGTATATGGATAAACGATATCAAGGTGCTGAGTTAC
AATTACTACAAAAAACTTGTAAAGAAAAAAATATTCCAATTCCTATTATT
GTTGAGAAACCAGATAATTTTAATCAAGTAGATAGAATACGT
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