mRNA_P-fluviatile_contig7.13480.1 (mRNA) Porterinema fluviatile SAG_2381

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-fluviatile_contig7.13480.1
Unique NamemRNA_P-fluviatile_contig7.13480.1
TypemRNA
OrganismPorterinema fluviatile SAG_2381 (Porterinema fluviatile SAG_2381)
Homology
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BLAST of mRNA_P-fluviatile_contig7.13480.1 vs. uniprot
Match: D7FME6_ECTSI (Similar to mitochondrial import inner membrane translocase,TIM17/22/23-like protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FME6_ECTSI)

HSP 1 Score: 205 bits (522), Expect = 1.400e-61
Identity = 114/169 (67.46%), Postives = 127/169 (75.15%), Query Frame = 2 
Query:  260 MGDWVADPAE-TPDAYSANCASRAAEGFYVGALYGTVWGFIGRAYSSPVFPSTPEMGGRSPEPTAEPKPARGASGGQPARNLLRALGRSAAGYGAATASTAGVFGVFVGIVNAGTCACERFRGKKDWINNMCGGAAAGLSVALLSRHTRSPRMVAAHVAGAAAITSVVA 763
            M DW  +P E T   Y+ +C+SRAAEGFYVGALYG VWG IGRA  +P  PSTP MG   P    + +PA G      +R + RA GR AAGYG A ASTAGVFGVFVG+VNAGTC CER RGKKDW NN  GGAAAGLSVALLSRHTR+P+M+AAHVAGAAAITSVVA
Sbjct:    1 MEDWNVEPPEMTHKDYAPDCSSRAAEGFYVGALYGAVWGVIGRAAHAPTLPSTPAMGELKPPSGLQAQPAPGLETRGVSRQV-RAFGRGAAGYGIAAASTAGVFGVFVGMVNAGTCGCERLRGKKDWFNNAFGGAAAGLSVALLSRHTRTPQMLAAHVAGAAAITSVVA 168
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BLAST of mRNA_P-fluviatile_contig7.13480.1 vs. uniprot
Match: A0A7S2MQ39_9STRA (Mitochondrial import inner membrane translocase subunit TIM22 n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2MQ39_9STRA)

HSP 1 Score: 66.6 bits (161), Expect = 1.640e-9
Identity = 53/154 (34.42%), Postives = 72/154 (46.75%), Query Frame = 2 
Query:  314 CASRAAEGFYVGALYGTVWGFIGRAYSSPVFPSTPEMGGRSPEPTAEPKPARGASGGQPARNLLRALGRSAAGYGAATASTAGVFGVFVGIVNAGTCACERFRGKKDWINNMCGGAAAGLSVALLSRHTRSPRMVAAHVAGAAAITSVVAIATG 775
            C S+ A+G   G L G VWG +        F  T      +P  +  P  ++    G   R  L ++GRS+ G           FG+F+GI NA +C  E+ RGKKDW+N+M GG AAG    L S    SPRM      G   +TS+  +  G
Sbjct:   23 CGSKFADGAARGLLIGLVWGAV--------FDVTSLHEPATPHRSNPPCSSQTILWGSKVRPALYSMGRSSIG-----------FGLFLGIFNAISCNSEKIRGKKDWMNSMFGGFAAGTFAGLGSG---SPRMTFISALGTGTVTSLFYVVQG 154
The following BLAST results are available for this feature:
BLAST of mRNA_P-fluviatile_contig7.13480.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D7FME6_ECTSI1.400e-6167.46Similar to mitochondrial import inner membrane tra... [more]
A0A7S2MQ39_9STRA1.640e-934.42Mitochondrial import inner membrane translocase su... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-fluviatile_contig7contigP-fluviatile_contig7:568302..573824 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Porterinema fluviatile SAG_23812021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons5
Model size1197
Cds size534
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817117.8111253-UTR-P-fluviatile_contig7:568301..5687051622817117.8111253-UTR-P-fluviatile_contig7:568301..568705Porterinema fluviatile SAG_2381UTRP-fluviatile_contig7 568302..568705 -
1693571200.3265355-UTR-P-fluviatile_contig7:568301..5687051693571200.3265355-UTR-P-fluviatile_contig7:568301..568705Porterinema fluviatile SAG_2381UTRP-fluviatile_contig7 568302..568705 -
1622817117.8706977-UTR-P-fluviatile_contig7:571825..5720161622817117.8706977-UTR-P-fluviatile_contig7:571825..572016Porterinema fluviatile SAG_2381UTRP-fluviatile_contig7 571826..572016 -
1693571200.3800633-UTR-P-fluviatile_contig7:571825..5720161693571200.3800633-UTR-P-fluviatile_contig7:571825..572016Porterinema fluviatile SAG_2381UTRP-fluviatile_contig7 571826..572016 -
1622817117.8823695-UTR-P-fluviatile_contig7:573756..5738241622817117.8823695-UTR-P-fluviatile_contig7:573756..573824Porterinema fluviatile SAG_2381UTRP-fluviatile_contig7 573757..573824 -
1693571200.3984733-UTR-P-fluviatile_contig7:573756..5738241693571200.3984733-UTR-P-fluviatile_contig7:573756..573824Porterinema fluviatile SAG_2381UTRP-fluviatile_contig7 573757..573824 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622817117.8248072-CDS-P-fluviatile_contig7:568705..5688741622817117.8248072-CDS-P-fluviatile_contig7:568705..568874Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 568706..568874 -
1693571200.337922-CDS-P-fluviatile_contig7:568705..5688741693571200.337922-CDS-P-fluviatile_contig7:568705..568874Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 568706..568874 -
1622817117.8394494-CDS-P-fluviatile_contig7:569330..5695331622817117.8394494-CDS-P-fluviatile_contig7:569330..569533Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 569331..569533 -
1693571200.3493047-CDS-P-fluviatile_contig7:569330..5695331693571200.3493047-CDS-P-fluviatile_contig7:569330..569533Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 569331..569533 -
1622817117.8497837-CDS-P-fluviatile_contig7:571218..5712801622817117.8497837-CDS-P-fluviatile_contig7:571218..571280Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 571219..571280 -
1693571200.3603709-CDS-P-fluviatile_contig7:571218..5712801693571200.3603709-CDS-P-fluviatile_contig7:571218..571280Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 571219..571280 -
1622817117.860375-CDS-P-fluviatile_contig7:571725..5718251622817117.860375-CDS-P-fluviatile_contig7:571725..571825Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 571726..571825 -
1693571200.3687072-CDS-P-fluviatile_contig7:571725..5718251693571200.3687072-CDS-P-fluviatile_contig7:571725..571825Porterinema fluviatile SAG_2381CDSP-fluviatile_contig7 571726..571825 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-fluviatile_contig7.13480.1prot_P-fluviatile_contig7.13480.1Porterinema fluviatile SAG_2381polypeptideP-fluviatile_contig7 568706..571825 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-fluviatile_contig7.13480.1

>prot_P-fluviatile_contig7.13480.1 ID=prot_P-fluviatile_contig7.13480.1|Name=mRNA_P-fluviatile_contig7.13480.1|organism=Porterinema fluviatile SAG_2381|type=polypeptide|length=178bp
MGDWVADPAETPDAYSANCASRAAEGFYVGALYGTVWGFIGRAYSSPVFP
STPEMGGRSPEPTAEPKPARGASGGQPARNLLRALGRSAAGYGAATASTA
GVFGVFVGIVNAGTCACERFRGKKDWINNMCGGAAAGLSVALLSRHTRSP
RMVAAHVAGAAAITSVVAIATGPPVGS*
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mRNA from alignment at P-fluviatile_contig7:568302..573824-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-fluviatile_contig7.13480.1 ID=mRNA_P-fluviatile_contig7.13480.1|Name=mRNA_P-fluviatile_contig7.13480.1|organism=Porterinema fluviatile SAG_2381|type=mRNA|length=5523bp|location=Sequence derived from alignment at P-fluviatile_contig7:568302..573824- (Porterinema fluviatile SAG_2381)
GCTTGCCCTCGCCCTCCTTGGTCTTGTACGTTGCGCATACCTCTAGCAGA
GATATCTCCTCACGACAGGTGCGTAAGTGTAGCCATTGCGTGCTAGAGTT
AGTGTCGTGGTGGTGTCAAATATTTTGCAAAAGAATCAGTCAACGGACGT
CAGATTATTCTTCATCGTCATGTGTTCCAACGAGCTATGCCATGCTCCCA
CCGCCGCCAGCCTTGACGCGTTTTCCGAGAGGCGATACCTCTCTGGCTCT
AACTAAGCTGTGTTTTTTGCGTTCTTGATTCCTGTGCGATGATCCCCGCA
GTGGATGACTGTCGTGTGAGTTTCTGGTTGTTGTTTGTTCGAGAAACCTG
GCCATGCCCTTAGCGGCACTTGTGGGTGTACAAGGTCTTTCTAGCTCTCT
AAGCGCCTTGCGGGTGCAGCTTTATCGTGGCAGACAGCAGTGCACAACGT
TCAGGAGTACCCTACGTGCAGTAGCGCACTACGTGCAGCATTAAGAAGGT
TTTTTTATCTTGTTGCGGGGATGCAATAATGCCGGCGTCCATGTCGGAGA
GGAGACGCCCGCCCGGACCCCTGGGAATCCCAACAACCGCCGGACACCCA
CCCCGAGGCCAGCGCACACTTTGTTGCTCTCGCGTCCCGTATATCAACGA
AAGTTGGCACGTCAAGGAGCGGTGGCAATAATATCTCTAGCCGTAGAAGT
ATACCAACAGCATGAGTCACCGTGTTGTCGGTACTGTGTCCACCATATGC
TTGCGCTTTGCTCGCTGTTTGCGGATTTCCTGGGCAGACAGCAGGTCGTT
GCCCTTTGGTCAATGAAGGGCGTCCCTCCCCCCCCATCATCTGAAATGGC
GCTCACTCACAACAACGCACACGATTACGTCAAATACGCGGACCAGCAGT
GTGCCGGTGGGCTGGCACACGCATGTTTCGTCCGACTCTGACCAAATAGG
AACATGCACGTACAGCTTGGTCTCCCCTCCCCGTGAAGATCTCGATTGCC
CCCGAAGGCTGAAAATGCGCAATGCACGGCACTAAGTTTGTAGGGCACAC
AGAGGCGCGCTCGCAAGAGAACGGCGCGTCCAGTTGCTATAGATTGCTGG
AGAAAGGGGGAAACTGCGGAGCTCAGCGTCGAGTGCAACACACAACTCTA
ACGATTGGCTTGGTTCGAGTCTGATGAGAAACCGAGGCAGCAATCTCAAG
GCCTAATTCTGCAGCTTATCGTGGTCTTGTGTGAGCGGGCCGGTGCTCTG
CACACGAGGAGATGGAGGACACCCTTTACTGTCAACAACAACCCGACAAA
TGGCGCGGGTGCAGTTTTGGATAGATTAGGAAAGCGACAGCCCCCCCGAA
AATGTTTGGCTTTCGTCGCCGCGAAAGCCGACCTCGCCACCCTGGTTTGG
ACAGTTCCATGGAACAATCGAGTTTGTTGCGACTTGGTCCGACGGGGAGG
GGGCGAGGACACACGGGATAGCCGAAAGCAACCGCATCCGAGCTGGTATA
GTATCTGGCTCTCAAAATGCATGGCGAAGTGAGACCTTAACAGCATGTGA
CTAAGCAAGCAGGAAAAAGGCGCCCAGAAATGACGAAATGACGACAGGCA
GCCGGAATGGTGTGTCGCTCTCGCATACATTCACGGAACCCCGTTTAGCC
GGCGGGCGTGGAGCACGTGTCCCCTGGTCGACAATTTTGGGGGTACGTGA
AAGGCCATTCACGCGTCAGCCTTCGAAACGGACCGAAAATCAGGAGACGT
TTGTGCTCAAAACAAGCCCGTTGTTGTTCATGCCCTTCGTTTTGCTGTCA
ACAAGCAGACATCCCACACGGAGTTTTGCTTGTCTCGTATGGACCAGGAG
GCAAGCCCAACACAGAAGAGCGGCAGCAGCACCACTACGACGACCACCAG
CTGCGGTAACGGCGAGAGCAAGTAAGCCGCACGAAGGAGAACAACAGCAG
CAGCAGCAGTAGCGGCGGTGGCGGCGGCGGCGGCGGCAACTGTGACAGCA
TGGGTGACTGGGTAGCTGACCCAGCAGAGACTCCCGACGCGTACAGCGCA
AACTGCGCGTCGAGAGCGGCGGAAGGTTTCTACGTAGGGGCTCTCTATGG
TACGCAGTATTTACCCCCGTCTAATTCCTTTGCTTAATGCCCTCTTGCCG
ATTGGTCCTGTGATTACCGAGGGTATATACCTGCTTTGGTCGGTGCGCGT
GCTCTATCACACCGTGTATAAAAAGGAAAGCCCATCTCTAGCACCGTGAA
CGTATGATTTTCCCCCTTGTGCTGAATGTTTTGCTGCTTACGTCAGGGGA
ATGAAAGCACGCGCGTCTCAAGTGGACTGGTTTCGGGGGTTTCTCTGTTT
GCTCCCTGCGGTCTTGGCGTCGTCATCGGAGAGCGCCCCGTTACGCCGCT
CAGAAACGCGCTGCTGTGCAACGCCCCCGTCGGCCTAATTTAACCTCCGA
AAGTCAACCACGCAAAAATGGTTGCGCTCCGCTCCGTTTCCTCTCTGGCC
TTCGCCGAAACGCAACAAAATTAAACGCCGCCGCCCTCGCTTAGGAACGG
TCTGGGGGTTCATCGGCCGCGCCTATTCTTCCCCGGTTTTTCCGTCGACG
CCAGAGGTAAGCCATTGTTGTAGTTCGTGCTTCAGAATGACTGCCATACA
CGGATTAGAGTTTTCGATGACTTCTTCACCAAGGTATGCGCACGGGTGGA
ACCCGCCGACGAATAGGGGATACATCGAGTCACTCATTGGTGCTTGACGC
GCACAAGGGACGAACGGACCAAATGGTTGCAATTCAAGAGGTGCTTTCTT
CACACGCGGGAGGGGGGGGAGTTCTTGGAACGGTTTCAACCCCCCCCTCC
CTGTGCCTCAGAGGTCTACAGCTGAACGGCATGCGTGGCGTGAGTACCCC
CCGCCCCCTTCTCCCGCTATCCCTCAGCGCGAGCAGGGAGCATGACTGAC
GAGGAACAGAACCACTCGCGTTGGTGCCGGCCAAGTTTAGTTCACGACTT
TGAAGGCGTTCGTTGAACGGGGCTTGGGTGTGTATGCGTTGTTCTTCTTG
CTTTCTTGTTTCACTTGCGGTTGCCATGGATACCGATAATTCCGTCAAAT
TCGGGTTACGTGAAGAATCTTCGCTGGCTACGCGCGCACTGTACGAAAGG
TAACCGGCAAAATTACACACGTCGGACGGATCACGATCTAGACCATCTGT
TGGCTACATACATCACGGCCAGCCAACATGTACAGATCATCGACGGGCTT
TACAGATCGCCTGTGACCAATGGTAATTCGATCCTCGACAGCATCGATCG
GTGGCCTGATCTGCCCGACGTGTGTGACACGTCGCACCAATCGTTGAGAA
GAAATATCCCTGTTGCTTTGCGAAGAGCCAAACGATGATGCCTATCTTCT
CCAGGACGAGCCGTCCACGGCTCTTAATGGAAAAGCTTTTCCTGAAGACA
TGCCGACGGCCGGGAGTTTTTTGTTTTTGTTATTTCCAATGACATCCCTT
CAAATCGTAAGAATTTCGTCCCGAAATTATCCGACGTTTTATGGAGCACG
TCTCCCCGCGGATCAATGAGGAACACGCGAGCTCATTTCCGTTCAAATCC
GAAAAAGCTTTGGTTTTGTTTTACCGAGGGGCCTTTCGATCTTAAGCTGC
ACGACTGGTTCGGATGAAAACACCCTGGTTCGTGTACACCAGGACGATTG
CACGCCGCCGCTCTCACCGCTGTGGGTGCCTGGTGCCGTTGCCCTTCGTA
AGTGCCGAAGAACTGCTCGGGCGTATACTACGCTTACGCTTTGATTTGAT
TTTTGCCTCCATGTTTCAGGAGTTGGGATACATCCAATATGGAGAAAAGG
CACTGACTCTTGGGCCTTTTATTTTATGTTATGATGAAGCGAAAATATTG
ATGCGATGTTTGTGTCTGCGATGTCTGTTCTTTGGTTTCAAAAGTGGAGA
CTCCCGCCCGCGCCCCAACTCATGCTCCCCACTCCCACACACCCCCTTTC
CCGTGCTCGACCAAACGCTTCTCCACCGTTCCCCATCTTTCCTCTTCACG
ACCACGGGTTCACGCTCGACGAGATGCCCTTAATATCCGTCCTGTCCCCT
CGCCCCCCTCCCGAGAACGTTGCTCTTCTCCGTGCCCGAGGAGACGCCCT
CCATCCACCGTCACCCCTTCCTTTGCGCTGCTTTTCTCGGCTAGACTCAC
GCCTGCACGTACTCCTCACCGGCTCCATCCAATCCAGCCCCACCACTTGT
CCACGCTCTCCTTCCCGCTGCTGCCGCCGCTTTCGAGATAGATGGGGGGA
AGGTCTCCGGAGCCGACGGCGGAACCCAAACCCGCACGAGGGGCGTCCGG
GGGCCAGCCGGCGCGAAATCTCCTCCGGGCCCTCGGGAGGAGTGCCGCCG
GGTACGGCGCGGCGACCGCGTCGACGGCGGGGGTGTTCGGGGTGTTCGTG
GGCATCGTGAACGCCGGGACGTGCGCGTGCGAGCGATTCCGAGGGTAGGT
TTGGTTGTGGTCGAAACGAGACGTTCTTTTTTCTTTGCAATGTGGACGCC
TACATGTTTGCCTCGTTTTCCTTGTTTTTTGTTTGTTCGAGGGGTCTTCG
AGCTGTCTAATTTCGGTGGAATGTACGCGGACTGCACATCTTGTTGTCGG
TGTTCCGGACGAAGACCTCTAGGATCACTGCGCGAAAATGAATGATGCAT
GGGGCCACACGGCGCCCGACACGGGTTGACTTGGGAGAACGATTTAATTA
GATGGATTGACTCAAGATAGCACCCCCCTCGAAAGATTTTCAAAGCTCCA
TTCCTCGAAATCATGAAGATCGCGGAGACCTTCCACTTGATTCTCTCAAC
CACAAACTTGGGGCATGTTCTTTATTGTGGCTGTTCCTCGACTCTGTTCC
GCGTTTGCGCTATTGCTGTTGAACATGTGCTTGCTGGCTGGCTGCTAAAG
GAAGAAGGACTGGATCAATAACATGTGCGGGGGCGCGGCGGCAGGGCTGA
GCGTGGCGCTGCTGTCGAGACATACACGTAGCCCCCGGATGGTCGCGGCT
CACGTCGCGGGGGCAGCCGCGATAACGTCGGTGGTTGCAATCGCGACCGG
TCCGCCGGTCGGAAGTTGAAGCAAAAGACACACTCCGGAGTGTACGTTTC
TTCCATGTGCAGGAACCCGGTTTATGAGCGCTGTTTTCTTTCCAAAGTTA
AATTCCACTCTGGCCTTTTCTCCTGGGGGCTTTCGTTTCTCTTTGCTGCA
GTCAAGAACAATGGGAGTGCCTGACGACTGGACAATCGCGACCGGTCAGC
CTGTCGGCAGTGGAAGCAAAAACCCAACAATCAGGAACCCCGTTTTTCGA
CGGGTTCTTTGAGACCATCGCCAACAGAAAATATGGAGTTCGGATCTGAG
GCAGCACTGTGGACACTCCCTGCCACCCACGCCCGCTCGAGAAACAAACT
CTCGTCGTGGTTGCACACAACGAAAATGAGCCAGCATAGGAGGCGACCCT
GTGGATACAACGCAATCTATTAG
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Coding sequence (CDS) from alignment at P-fluviatile_contig7:568302..573824-

>mRNA_P-fluviatile_contig7.13480.1 ID=mRNA_P-fluviatile_contig7.13480.1|Name=mRNA_P-fluviatile_contig7.13480.1|organism=Porterinema fluviatile SAG_2381|type=CDS|length=1068bp|location=Sequence derived from alignment at P-fluviatile_contig7:568302..573824- (Porterinema fluviatile SAG_2381)
ATGGGTGACTGGGTAGCTGACCCAGCAGAGACTCCCGACGCGTACAGCGC
AAACTGCGCGTCGAGAGCGGCGGAAGGTTTCTACGTAGGGGCTCTCTATG
ATGGGTGACTGGGTAGCTGACCCAGCAGAGACTCCCGACGCGTACAGCGC
AAACTGCGCGTCGAGAGCGGCGGAAGGTTTCTACGTAGGGGCTCTCTATG
GAACGGTCTGGGGGTTCATCGGCCGCGCCTATTCTTCCCCGGTTTTTCCG
TCGACGCCAGAGGAACGGTCTGGGGGTTCATCGGCCGCGCCTATTCTTCC
CCGGTTTTTCCGTCGACGCCAGAGATGGGGGGAAGGTCTCCGGAGCCGAC
GGCGGAACCCAAACCCGCACGAGGGGCGTCCGGGGGCCAGCCGGCGCGAA
ATCTCCTCCGGGCCCTCGGGAGGAGTGCCGCCGGGTACGGCGCGGCGACC
GCGTCGACGGCGGGGGTGTTCGGGGTGTTCGTGGGCATCGTGAACGCCGG
GACGTGCGCGTGCGAGCGATTCCGAGGATGGGGGGAAGGTCTCCGGAGCC
GACGGCGGAACCCAAACCCGCACGAGGGGCGTCCGGGGGCCAGCCGGCGC
GAAATCTCCTCCGGGCCCTCGGGAGGAGTGCCGCCGGGTACGGCGCGGCG
ACCGCGTCGACGGCGGGGGTGTTCGGGGTGTTCGTGGGCATCGTGAACGC
CGGGACGTGCGCGTGCGAGCGATTCCGAGGGAAGAAGGACTGGATCAATA
ACATGTGCGGGGGCGCGGCGGCAGGGCTGAGCGTGGCGCTGCTGTCGAGA
CATACACGTAGCCCCCGGATGGTCGCGGCTCACGTCGCGGGGGCAGCCGC
GATAACGTCGGTGGTTGCAATCGCGACCGGTCCGCCGGTCGGAAGTTGAG
AAGAAGGACTGGATCAATAACATGTGCGGGGGCGCGGCGGCAGGGCTGAG
CGTGGCGCTGCTGTCGAGACATACACGTAGCCCCCGGATGGTCGCGGCTC
ACGTCGCGGGGGCAGCCGCGATAACGTCGGTGGTTGCAATCGCGACCGGT
CCGCCGGTCGGAAGTTGA
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