mRNA_P-fluviatile_contig18.3170.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: D7FUT0_ECTSI (Methionine synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUT0_ECTSI)

HSP 1 Score: 2227 bits (5771), Expect = 0.000e+0
Identity = 1110/1230 (90.24%), Postives = 1167/1230 (94.88%), Query Frame = 2

Query:  305 KDYHILVKGNNDMLSITQPHIIKEIHTKYLESGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPPREPFMWLSGLEDLVVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVKAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEKALKNKGKATVETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPAATVDGEDVEVYANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            +DYHILVKGNNDMLSITQPHIIKEIHTKY++SGSDII TNTFS+TTIAMADYEMEKDVYELNFESARLAREAC EATAKDP+KPRFVMG+LGPTNRTGSISPDVEDPSFRNVTFDELV+AYKDQTKGLIDGG DIIIVETIFDTLNAKA VFAVREYFDEQG+TDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFC+GLNCALGA+QMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYD+TPEDMARDNLLFAEEGLINMLGGCCGSTP HIK        C+   +  PPREPFMWLSGLEDL+VTKERFQFLNVGERCNVSGSIRFKKLILKGDYQ AMDVAKQQVEDGAMVIDINLDDGLLDG+AAMQKFCKIAVTEPDV+KVPFMIDSSKF IVEAGLKWVQGKSIVNSISLKVGE++F+RHAR+VKAHGAAVVVMAFDE+GQAADCDNKVRICKRSYD+LV+EVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIK +CPYCKISGGVSNLSFGFRGVNVIRE+MHSVFL+HAVKAGMDMGIVNAGML VYDDIEPGLLKIVEDAVNN+H GATEALLDAAEIEKEKALKNKGK TVE SGWRTQ V  RLTHSLIKGIDQWVIEDVEECRLMLD +GKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEK AKMIADGIDP S++ +DDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYK       VYDMGVMQPCD ILAKA +LKVD++GLSGLITPSLDEMVFVAKEM+K G KLPLLIGGATTSKMHTAVKISPQYA+PE PVIHVLDASRSVTVVGALLSDRK DYVEE+MEEYEELREEHYAGQEERHYL FEKA +KRK IDF+K PP P P +PG+TVVTG Y LDDVLDYIDWNPFFQTWELRGRYPNRGYPKIF DE+VGEEAKKLF +AQ MLK+I++EK+L+L G+V IFPAATVDGEDVEVYAN+SREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFA+ACMGCEKMVKEFE+LNDDYSKIMVQALTDRFVEAFAEKLHED+RK IWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLN AELTGIELSESLSMMPASSVSALVFAHP+SEYFAVGTI KDQVSSYAERKG+D+ TCEKWLSPIL+YDRD
Sbjct:    3 QDYHILVKGNNDMLSITQPHIIKEIHTKYVDSGSDIIATNTFSATTIAMADYEMEKDVYELNFESARLAREACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGADIIIVETIFDTLNAKAGVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAEEGLINMLGGCCGSTPAHIKAMKDAVEPCSTR-ALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQERLTHSLIKGIDQWVIEDVEECRLMLDDQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYK-------VYDMGVMQPCDLILAKAKQLKVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKNIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVAIFPAATVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAIACMGCEKMVKEFEDLNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1224          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A7S2XWE9_9STRA (Methionine synthase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XWE9_9STRA)

HSP 1 Score: 1865 bits (4832), Expect = 0.000e+0
Identity = 954/1286 (74.18%), Postives = 1078/1286 (83.83%), Query Frame = 2

Query:  143 GSVANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKYKLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLESG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPPR-EPFMWLSGLEDLVVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVKAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEKALKNKGKATVETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPAATVDGEDVEVYANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            G+V NYPYF R KDS  AYL RLFQE I ++DGAMGTMIQKYKLGEEEYRGERFKDYH LVKGNNDMLSITQP IIKEIH KYLE G S +IGTNTFSSTTIAMADY+ME  V ELN ES RLAREAC E TAKDP++PRFV+G++GPTNRTGSISP+VEDPS RNVTFDEL IAY +Q  GL++GG DI++VETIFDTLNAKAAVFA   + +E GL D+PLFVSGTLVDQSGRTLSGQTTE FYVS+RHCKPFC+GLNCALGATQM+PFL+RLA VAECFVHVYSNAGLPNAMGGYDDTP+DMAR+NL+FA+E L+NM+GGCCGSTPPHIK  AI  +   + P   P + EP +WLSGLEDLVV+K+RF FLNVGERCN++GSIRFKKLIL GDY  AMD+AK+QVEDGAMV+DIN+DDG+LDG+AAMQKF KIAVTEP+++KVPFMIDSSKFEIVEAG+KWVQGKSI+NSISLKVGE++F+R A ++K HGAAV+VMAFDE+GQAA    KVRICKRSYDILVNEV FPPEDIIFDPNILTIATGI+EHNNY VDFI+AT++IK +CPY +ISGGVSNLSFGFRGVNVIREA+HSVFLYHAV AGMDMGIVNAGM+ VYDDI   LL++VEDAV N+H  ATE LL+ +  EKEKA    G    E   WR + V  RLT++LIKGI Q++ EDVEECR  +D    RPL VIEGPLM GMN+VGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFME EK+A + A GI  + +  DDDS YAGKVL+ATVKGDVHDIGKNIV+VVLGCNNYK       V+D+GVMQPC+ IL    + + D+VGLSGLITPSLDEMVFVAKEM KAG K PL+IGGATTSKMHTAVKISPQYA  E PVIHVLDASRSVTVV +LL D K ++VE+LMEEYE+LREEHYAG EER +L  ++A+ K  +IDF   PP  AP Q GVTV+   Y L+DVL YIDWNPFFQTWELRGRYPNRGYPKIF DE VG EAKKLF++AQ MLK I+  K L L GV GIF A  V GEDVEVY    + EV A+FCMLRQQAEKES+ PYLS ADFIAPK+SG+ DYMGMFAVACMG E+    FE  NDDYSKIMVQAL DR VEAFAE LH D+R  IWGYAPDEK+  +DL K++YQGIRPAPGYPSQPDHTEK TMW+LL V E +GI LSE+LSM+PASSVSAL FAHP++EYFAVG I KDQV  YAERKG++VA  EKWLSPILNYDR+
Sbjct:   11 GTVENYPYFNREKDSVRAYLTRLFQEEIAIYDGAMGTMIQKYKLGEEEYRGERFKDYHTLVKGNNDMLSITQPQIIKEIHLKYLEEGNSQLIGTNTFSSTTIAMADYDMESYVRELNVESVRLAREACDEVTAKDPTRPRFVVGAIGPTNRTGSISPNVEDPSVRNVTFDELEIAYYEQVCGLVEGGCDILMVETIFDTLNAKAAVFACNRFLEEHGLEDMPLFVSGTLVDQSGRTLSGQTTEAFYVSIRHCKPFCVGLNCALGATQMKPFLRRLAVVAECFVHVYSNAGLPNAMGGYDDTPDDMARENLIFADEKLVNMIGGCCGSTPPHIK--AISEAVKGMPPRPLPEKKEPVLWLSGLEDLVVSKDRFTFLNVGERCNIAGSIRFKKLILGGDYATAMDIAKKQVEDGAMVLDINVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGMKWVQGKSIINSISLKVGEEEFKRQAGIIKRHGAAVIVMAFDEEGQAATESEKVRICKRSYDILVNEVQFPPEDIIFDPNILTIATGIEEHNNYGVDFIEATKKIKEICPYARISGGVSNLSFGFRGVNVIREAIHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPKDLLQLVEDAVLNRHSEATEKLLERSLEEKEKAASGGGPKKKEVE-WRIKPVTERLTYALIKGISQYIDEDVEECRQTVD----RPLQVIEGPLMQGMNIVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKKAALEAKGI--TEMPEDDDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYK-------VFDIGVMQPCESILDAIKKYQPDVVGLSGLITPSLDEMVFVAKEMEKAGLKQPLMIGGATTSKMHTAVKISPQYANNEHPVIHVLDASRSVTVVQSLLGDDKDEFVEDLMEEYEDLREEHYAGLEERCFLALDQAKQKGLQIDFAACPPVQAPNQLGVTVLDS-YTLEDVLPYIDWNPFFQTWELRGRYPNRGYPKIFNDENVGGEAKKLFDEAQEMLKDIVANKRLHLKGVCGIFAANRV-GEDVEVYTGADKSEVAAKFCMLRQQAEKESEAPYLSMADFIAPKDSGLDDYMGMFAVACMGAEEEAARFEAENDDYSKIMVQALADRLVEAFAEALHRDMRVSIWGYAPDEKLPAEDLHKIRYQGIRPAPGYPSQPDHTEKTTMWNLLQVEEKSGIRLSEALSMLPASSVSALCFAHPQAEYFAVGKIEKDQVVEYAERKGMEVAEMEKWLSPILNYDRE 1278          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A836CPG7_9STRA (Methionine synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CPG7_9STRA)

HSP 1 Score: 1837 bits (4759), Expect = 0.000e+0
Identity = 944/1284 (73.52%), Postives = 1057/1284 (82.32%), Query Frame = 2

Query:  146 SVANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKYKLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLESGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPPREPFMWLSGLEDLVVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVKAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEKALKNK--GKATVETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPAATVDGEDVEVYANESREEVLARFCMLRQQAEKESD-DPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYD 3988
            SVA+YP + +GKDS  AYL RLFQERIVM+DGAMGTMIQK++L EE+YRGERFKDY  LV+GNND+LSITQP II++IH +YLESGSDIIGTNTFSSTTIA ADY ME  VYELN+ESA+LAR AC E TA+DPS+PRFV GS+GPTNRT SISP VEDPS+RNV FDELV AY  + K L++GG DIIIVETIFDTLNAKAAVFAVR+Y  E    D+PL +SGTLVDQSGRTLSGQT EGFYVSMRH KPFC+GLNCALGA QM+PFL+RL+ VAECFVHVYSNAGLPNAMGGYDDTP DMARDN  FA EG++NM+GGCCGSTP HIK       +CA  P   P   P MWLSGLE L V+K+R  FLNVGERCN SGSI+FKKLI+ G+Y +AMDVAK QVEDGAMV+DINLDDGLLDGLAAMQKFCKIAVTEPDV+KVPFMIDSSKFEI+EAGLKWVQGK IVNSISLKVGE +FRR A +V+ HGAAVVVMAFDE+GQAAD ++KVRICKRSYDILV+E+ FPPEDIIFDPNILTIATGIDEHNNYA+DFI AT RIK +CP+CKISGGVSNLSFGFRGVN IREAMHSVFLYHA++AGMDMGIVNAGML VYDDI P LLKIVEDAV NKHPGATEALL+ A+IE+E A   K  GK  V+   WR Q V ARLTHSLIKGIDQ++  D EE R   D    RPL VIEGPLM GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFME EK  K +  G+DP+    DDD+ YAGKV+IATVKGDVHDIGKNIVAVVLGCNNYK       VYD+GVM  C+ IL    + K DIVGLSGLITPSLDEMVFVA+EM+K GC  PL+IGGATTSKMHTAVKI+PQY++PE PVIHVLDASRSVTVV +LL   K +Y EE++EEYEELRE            T EKA  KR +I+F+ TP AP P       V   Y+L+ VLDYIDWNPFFQTWELRGRYPNRGYPKIF D  VG EAKKLF+DAQ ML  I+  K L LTGV+G++PA  V GEDV +Y++ SR E LA FCMLRQQAEK++  + YLS ADF+AP   G  D++GMFAVACMGC+  VK FE  NDDYSKIMVQAL DR VEAFAE LH D+R++IWG+A +EKV G+DLFKLKYQGIRPAPGYPSQPDHTEKRTMW++L   EL GI+LSESLSMMPASSVSALVFAHP+SEYFAVG IGKDQV SYA RKG+ +  C++WL PILNYD
Sbjct:   14 SVADYPRYTKGKDSCTAYLTRLFQERIVMYDGAMGTMIQKHRLEEEDYRGERFKDYDKLVRGNNDLLSITQPQIIRQIHIEYLESGSDIIGTNTFSSTTIAQADYGMENLVYELNYESAKLARSACDEVTARDPSRPRFVAGSIGPTNRTASISPSVEDPSYRNVNFDELVDAYYFEAKALVEGGSDIIIVETIFDTLNAKAAVFAVRKYLRETNQEDIPLMISGTLVDQSGRTLSGQTGEGFYVSMRHVKPFCIGLNCALGAQQMKPFLRRLSMVAECFVHVYSNAGLPNAMGGYDDTPSDMARDNHEFASEGMVNMIGGCCGSTPQHIKAMHEDVKDCAPRPLPKPLDPPVMWLSGLEPLQVSKDRINFLNVGERCNCSGSIQFKKLIMAGNYSSAMDVAKAQVEDGAMVVDINLDDGLLDGLAAMQKFCKIAVTEPDVSKVPFMIDSSKFEILEAGLKWVQGKCIVNSISLKVGETEFRRQATIVRDHGAAVVVMAFDEEGQAADEESKVRICKRSYDILVDELKFPPEDIIFDPNILTIATGIDEHNNYAIDFINATRRIKEVCPHCKISGGVSNLSFGFRGVNTIREAMHSVFLYHAIQAGMDMGIVNAGMLQVYDDIAPDLLKIVEDAVLNKHPGATEALLERAQIERELADARKAGGKVAVKEGSWRDQPVKARLTHSLIKGIDQYIETDTEEARSQAD----RPLSVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKRLKALEQGVDPNEP-VDDDAAYAGKVVIATVKGDVHDIGKNIVAVVLGCNNYK-------VYDLGVMVSCEDILKAVDKYKPDIVGLSGLITPSLDEMVFVAREMAKKGCTQPLMIGGATTSKMHTAVKIAPQYSSPEHPVIHVLDASRSVTVVSSLLGGEKTEYAEEVLEEYEELREXXXXXXXXXXXXTLEKAMGKRLEINFEATP-APQPPSFIGNKVLDNYSLESVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDATVGAEAKKLFDDAQVMLNDIVANKRLRLTGVLGMYPANRV-GEDVVLYSDASRAEKLATFCMLRQQAEKDTPTEAYLSLADFVAPV--GHRDHLGMFAVACMGCDDEVKRFEAENDDYSKIMVQALADRLVEAFAEALHADVRREIWGFASEEKVAGEDLFKLKYQGIRPAPGYPSQPDHTEKRTMWNVLKAEELAGIQLSESLSMMPASSVSALVFAHPQSEYFAVGKIGKDQVESYAARKGMPLDECQRWLGPILNYD 1281          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A7S4DBJ3_HETAK (Methionine synthase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBJ3_HETAK)

HSP 1 Score: 1830 bits (4741), Expect = 0.000e+0
Identity = 938/1298 (72.27%), Postives = 1067/1298 (82.20%), Query Frame = 2

Query:  134 EPKGSVANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKYKLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLESG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPPRE------PFMWLSGLEDLVVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVKAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEKALKNKGKATVETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLS-DRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPAA-TVDGEDVEVYANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESG--VADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            E KGSV NYPYF R  DS  AYL RLFQE+IVM+DGAMGTMIQKYKL EE+YRGE+FKD+H LVKGNNDMLS+TQP IIKEIH +YL  G S +IGTNTFS TTIAMADYEME  V ++NF SAR+ARE   E TA DP++PRFV+G++GPTNRTGSISP VED S RNVTFDEL  AY  QT  L++GG D+IIVETIFDTLNAKAA+FAV  YF+E G T+ PLF+SGTLVDQSGRTLSGQT E FY S+RH KPFC+GLNCALGA QM+PFL+RLA V ECFVHVYSNAGLPNAMGGYDDTPEDMAR+N +FA E L+NM+GGCCGSTPPHI   A             PPRE      P MWLSGLEDL+V+K+RF FLNVGERCN++GSI FKK I+ GDY AAM++AK+QVEDGAMVID+N+DDG+LDG+AAMQKF KIAVTEP+++KVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGE++F+RHA + K HG A+VVMAFDE+GQAA  D+KV +CKRSYDILV+EVGFPPEDIIFDPNILT+ATG++EHNNY VDFI AT RIK  CP+ KISGGVSNLSFGFRGVNVIREAMHSVFLYHAV AGMDMGIVNAGM+ VYDDI   LL +VEDAV N+ P ATE LLD + +E+EK     GK  V+   WRT+ V  RLTH+LIKGID+++  DVEECR  +     +PL+VIEGPLM+GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFMEAEK AK+ A G+DP     D+DS YAGKVL+ATVKGDVHDIGKNIV+VVLGCNNYK       V+D+GVM  C+ ILAKA E KVD++GLSGLITPSLDEMVFVAKEM+K G K PL+IGGATTSKMHTAVKI+PQYAT E PVIHVLDASRSVTVV +LLS  +K  YVEEL+EEYEELREEHYA  EER YL F K + K  ++DF   PP  AP  PGV  + G Y L DV+ +IDWNPFFQTWELRGRYPNRGYPKIF DEAVG EA+KLF+DAQAML+ I++ K L LTGV  IFPAA T DGEDVEV++ E     L +FCMLRQQAEKE+D+PYLS ADF+AP+  G  V D++G FAVAC+G ++     E  NDDY+KIMVQAL DR VEAFAEKLH DIR  +WGYAPDE++  +DL K++YQGIRPAPGYPSQPDHTEKRTMWDLL V EL+GI LSESLSMMPASSVSALVFAHP+++YFAVG IG+DQV  YA RKG+DV   EKWLSPILNYDRD
Sbjct:   11 ETKGSVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAIAADVE-------GMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGKV-VKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAV----AKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEAEKAAKLAAQGLDPQEA--DEDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYK-------VFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEG-YELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGHSSLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEAARHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 1286          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A097PA47_9STRA (Methionine synthase n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A097PA47_9STRA)

HSP 1 Score: 1814 bits (4699), Expect = 0.000e+0
Identity = 920/1285 (71.60%), Postives = 1068/1285 (83.11%), Query Frame = 2

Query:  155 NYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKYKLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLESGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTD-VPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPPREPFMWLSGLEDLVVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVKAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEKALKNKGKATVETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPAATVDGEDVEVYANESR----EEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            +YP F RG+DST+AY ++LF+ERIV++DGAMGTMIQK++L EE+YRGERFKDYH LV+GNND+L++TQP IIK+IH KYL+ G+D+IGTNTFSSTTIA ADY+ME   YELN ESAR+ARE   E +A++P +PRFV GS+GPTNRT SISPDV D S RNVTFDEL  AY ++ +GLIDGG D II+ETIFDTLNAKAA+F+VR    E+G+ + +PL +SGTLVDQSGRTLSGQTTE FYVS+RHC PFC+GLNCALGA QMRPFLQRL+KVAECFV VYSNAGLPNAMGGYDDTP DMA DN +F +E L+NM+GGCCGSTP HI   A       + P     R   MWLSGLEDLVV+KERFQFLNVGERCN++GS  FKKLIL GDY  AMD+AK+QVEDGAMV+D+N+D+GLLDGLAAMQ+F KIA+TEP+ +KVP M+DSSKFEI+EAGLKWVQGK IVNSISLKVGE++F+R A VVKAHGAAVVVMAFDE+GQAAD  NKVRICKRSYDILV+EVGFPPEDI+FDPNILTIATGI+EHNNYAVDFI+A   IK +CP+ KISGGVSNLSFGFRGVNVIREAMHSVFLYHAV+AGMDMGIVNAGML VY DI P LL+IVEDAV NKH  ATEALL+ + +E+EKAL NKG+AT   + WRT +V ARLTH+L+KGID+++ EDVEECR ++     RPL+VIEGPLMSGMNVVGDLFG+GKMFLPQVIKSARVMKKAVA+LLPFME EKE KM   GI+  +   DDDS YAG VL+ATVKGDVHDIGKNIV VVLGCNNYK       VYDMGVM PC+KI+ KAIE+K  ++GLSGLITPSLDEMVFVAKEMSKAG  +PLLIGGATTSKMHTAVKI+P Y+T E P IHVLDASRSVTVVG+LL + K +YV+++++EY+ELREEHYAG EERH  TF+ A+     IDF  +PP  +P+      V   Y+L+DV+ +IDWNPFFQTWELRG+YPNRGYP+IF DE VGEEAKKLF DAQA+LK +++ K LSL GVVG+FPAA V GEDVEVYA+E      EE + +FCMLRQQ EKESDD Y S ADF+A KESG  DY+G FAVAC G E+ V+ +E LNDDY+KIMVQAL DRFVEAFAE LH ++R K WGYAP+E+++ +DL K+KY GIRPAPGYPSQPDHTEK  MW+LL   EL GI L+ESLSM PASSVSALVFAHPES+YFAVG I KDQV  YA RKG+ V   E+WL+PILNYDRD
Sbjct:   13 DYPRFVRGRDSTLAYFHKLFRERIVIYDGAMGTMIQKHRLTEEDYRGERFKDYHKLVRGNNDLLTLTQPTIIKDIHRKYLKGGADLIGTNTFSSTTIAQADYDMESLAYELNVESARIAREVADEVSAEEPERPRFVGGSIGPTNRTASISPDVVDASKRNVTFDELDEAYYEEARGLIDGGCDFIIIETIFDTLNAKAAIFSVRRLLKERGMEETMPLIISGTLVDQSGRTLSGQTTEAFYVSVRHCNPFCVGLNCALGAKQMRPFLQRLSKVAECFVSVYSNAGLPNAMGGYDDTPADMAADNSVFVKESLVNMIGGCCGSTPDHINAIAKMCE--GVPPRPVHERVKHMWLSGLEDLVVSKERFQFLNVGERCNIAGSPLFKKLILAGDYAKAMDIAKKQVEDGAMVLDVNMDEGLLDGLAAMQRFLKIAMTEPECSKVPIMVDSSKFEIIEAGLKWVQGKCIVNSISLKVGEEEFKRQAAVVKAHGAAVVVMAFDEEGQAADEYNKVRICKRSYDILVDEVGFPPEDIVFDPNILTIATGIEEHNNYAVDFIKACRTIKEVCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVQAGMDMGIVNAGMLQVYSDIPPDLLQIVEDAVLNKHAEATEALLERSMVEREKALANKGQATAVQAPWRTLEVQARLTHALVKGIDEFIDEDVEECRQLVP----RPLEVIEGPLMSGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPFMEKEKEEKMKEQGIEAVA---DDDSAYAGVVLLATVKGDVHDIGKNIVGVVLGCNNYK-------VYDMGVMIPCEKIIEKAIEVKAQVIGLSGLITPSLDEMVFVAKEMSKAGMTVPLLIGGATTSKMHTAVKIAPNYSTVEHPCIHVLDASRSVTVVGSLLGEDKEEYVQDVLDEYDELREEHYAGLEERHLKTFDAAKKLGFNIDFGASPPVCSPVGGTGVKVVDYYSLEDVVPFIDWNPFFQTWELRGKYPNRGYPRIFNDENVGEEAKKLFADAQALLKDLVDNKRLSLRGVVGVFPAARV-GEDVEVYASEEAKDRGEEAIGKFCMLRQQQEKESDDAYFSLADFVASKESGHKDYLGAFAVACFGAEEEVERYEALNDDYNKIMVQALADRFVEAFAEALHREMRMKTWGYAPEEELDPEDLLKIKYDGIRPAPGYPSQPDHTEKVMMWELLKARELAGIGLTESLSMTPASSVSALVFAHPESQYFAVGRIEKDQVEDYAVRKGMVVEEVERWLAPILNYDRD 1280          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A1Z5K9H8_FISSO (Methionine synthase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K9H8_FISSO)

HSP 1 Score: 1701 bits (4405), Expect = 0.000e+0
Identity = 876/1299 (67.44%), Postives = 1037/1299 (79.83%), Query Frame = 2

Query:  152 ANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKY----KLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLESG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPP-REPFMWLSGLEDLVVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAV-KAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEK--ALKNKGKAT-VETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPA-ATVDGEDVEVYANESREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            A++P++ RG+DS   ++ +LF  RI ++DGAMGTMIQ Y    KL E+EYRGERFKD+   VKGNNDMLSITQPHII+ I+ +YLE G SD+IGTNTFSSTTIAMADYEME   YELN+  ARLARE C E TA+DP+KPRFV+G++GPTNRTGSISP VEDPS RNVTFDELV  Y +Q  GL+DGG DI++VETIFDTLNAKAA++A+ EY +  GL D+P+FVSGTLVDQSGRTLSGQT E FY S+RH KP C+GLNCALGA  M PF++RLA   ECF+HVYSNAGLPNAMGGYDD+PE MA  N LF E G +NM+GGCCGSTPPHI  RAI        P   P    P MWLSGLED+ V          FLNVGERCN++GSI+FKKL++ GDY AAMD+AKQQVEDGA VIDIN+DDG+LDGL AMQKF KIAVTEP+VAKVPFM+D+SKFEIV AGLKW QGK IVNSISLKVGE+ F  HA ++K HGAAVVVMAFDE+GQAA    KVRICKRSYDILVN+V FPPEDIIFDPN+LTI TG++EH NY VDFI A + IK  CPY KISGG+SNLSFGFRGV  IRE++H+VFL+HA+ ++GMD+GIVNA  +++ +++EP LLK  E+ V NK   ATE +L+    EKE+  A K  G  T    + WR QD   RL H+LI GI ++V +DVEE R     +  +PLDVIEGPLM GMN+VGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFM+ EK   +IA G++P   + +DDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNN+K       VYD+GVM  C+ IL KA E  VD++GLSGLITPSLDEMV VAKE+SK G K PLLIGGATTSKMHTAVKI+P Y++ E PVIHVLDASRSVTVV +LL + K DYV ++ EEYEE+RE++YAG E+R++LTFE+A+ ++  IDF+K+PPA  P Q G T++  K  L DV+ YIDWNPFFQTWELRGRYPNRGYPKIF DEAVG+EAKKLF+DAQ+M+K I+ + ++ LTGVVG++PA  + DGEDVEVY NE+  E     ARFCMLRQQAEKESDDPY SQADF+AP+  G+ D++GMFAV+C+GC+ +V ++E  NDDYSKIM QAL DRFVEAFAE LH +IR  +WGYAPDEK+   DL K+KY GIRPAPGYPSQPDHTEK TMW+L+   EL GIELSESLSMMPASSVSALVFAHP++EYFAVG +GKDQV SYAERK + +  CE+WLSPILNY+R+
Sbjct:   80 ASFPHYVRGRDSLRKHITQLFTSRIAIYDGAMGTMIQNYAKKNKLEEDEYRGERFKDWKCNVKGNNDMLSITQPHIIQGIYRQYLEEGGSDLIGTNTFSSTTIAMADYEMEAYAYELNYWGARLAREVCDEVTARDPAKPRFVVGAIGPTNRTGSISPSVEDPSARNVTFDELVETYFEQVVGLMDGGADILMVETIFDTLNAKAALYAIGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAQHMTPFVERLAGCVECFMHVYSNAGLPNAMGGYDDSPETMAEQNRLFFENGWLNMVGGCCGSTPPHI--RAIREVSEGYEPRKLPDVGRPKMWLSGLEDMKVEDVHNHLGLPFLNVGERCNIAGSIKFKKLMMAGDYGAAMDIAKQQVEDGAHVIDINVDDGMLDGLTAMQKFVKIAVTEPEVAKVPFMLDASKFEIVLAGLKWCQGKPIVNSISLKVGEEMFIEHATLLKKHGAAVVVMAFDEEGQAATESEKVRICKRSYDILVNKVKFPPEDIIFDPNVLTIGTGMEEHANYGVDFINACKTIKEECPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAILESGMDVGIVNAKEMLMIEEVEPELLKASENLVFNKSEDATEVMLELTRKEKERFEAFKAGGGVTKTNEASWRDQDAVKRLEHALINGISEFVDQDVEEAR----QKCSKPLDVIEGPLMDGMNIVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMDEEKRQNLIAQGLNPDDFDQNDDSNYAGKVLMATVKGDVHDIGKNIVAVVLGCNNFK-------VYDIGVMCSCELILEKAKEYDVDVIGLSGLITPSLDEMVVVAKELSKGGFKQPLLIGGATTSKMHTAVKIAPNYSSAEHPVIHVLDASRSVTVVSSLLGNAKEDYVADIHEEYEEMREDYYAGLEDRYFLTFEQAKQQKFMIDFEKSPPASQPKQIGTTLID-KVKLSDVVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGKEAKKLFDDAQSMMKEIIADGSMWLTGVVGLYPANRSDDGEDVEVYENEADRETGTPKARFCMLRQQAEKESDDPYFSQADFVAPR--GMKDHLGMFAVSCLGCDALVDKYEKSNDDYSKIMAQALADRFVEAFAEYLHREIRVNMWGYAPDEKLNEADLLKIKYDGIRPAPGYPSQPDHTEKTTMWELIKAKELAGIELSESLSMMPASSVSALVFAHPQAEYFAVGQVGKDQVVSYAERKNMKLDVCERWLSPILNYERE 1361          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A7S1ZPU5_9STRA (Methionine synthase n=3 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZPU5_9STRA)

HSP 1 Score: 1692 bits (4383), Expect = 0.000e+0
Identity = 875/1303 (67.15%), Postives = 1039/1303 (79.74%), Query Frame = 2

Query:  155 NYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKY----KLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLE-SGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPPRE------PFMWLSGLEDLVVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAV-KAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKE--KALKNKGKAT-VETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPA-ATVDGEDVEVYANESREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            +YP+F RG+DS   Y+  LF  +I ++DGAMGTMIQ Y    KL EEEYRG+RFKD++  VKGNNDMLSITQPH+I  I+ +YLE  GS++IGTNTFSSTTIAMADY ME   YELN+E ARLAR+AC E TA DP+ PRFV+G++GPTNRTGSISP VEDPS RNVTFDELV AY +Q  GL+DGG DI++VETIFDTLNAKAA++AV EY +  GL D+P+FVSGTLVDQSGRTLSGQT E FY S+RH KP C+GLNCALGA  M PF+++LAKV ECF+HVYSNAGLPNAMGGYD++P+DMAR+N  F E G +NM+GGCCGSTPPHIK       ECA    +  PR+      P MWLSGLEDLVV          FLNVGERCN+SGSIRFKKL++ GDY  AMD+AK+QVEDGA V+DIN+DDG+LDGLAAMQKF KIAVTEP+V+KVPFM+D+SKFEIV AGLKW QGK IVNSISLKVGE+ F+  A ++K HGAAVVVMAFDE+GQAA  D KVRICKRSYDILVNEV FPPEDIIFDPN+LTI TG++EH NY VDFI A + IK  CPY KISGG+SNLSFGFRGV  IRE++H+VFL+HA+ ++GMD+GIVNA  ++   ++E  L K  E+ V NK P AT+A+L+  + EK   +A K  G  T ++   WR  +   RL HSL+ GI Q++ +DVEE RLM      +PLDVIEGPLM GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFME EK+  +I  G+DP   +P+DDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNNYK       VYD+GVM  C+ IL KA E  VD++GLSGLITPSLDEMV VAKEMSKAG K PLLIGGATTSKMHTAVK+SP Y T E PVIHVLDASRSVTVV +LL + K D+V ++MEEY+E+RE++YAG E+R++LTFE+A+ ++ KIDF    PAPAP + G+TVV     L++V+ YIDWNPFFQTWELRGRYPNRGYPKIF DEAVG EA+KLF+DAQAMLK I+ EK++ L GVVG+FPA  + DGEDV +Y++E         A FCMLRQQAEKE++DPYLSQADFIAP  +G  D++GMFAV+C GCE +V+++E  +DDYSKIM QAL DRFVEAFAE LH+ IR ++WGYA +E++   DL K+KY GIRPAPGYPSQPDHTEK TMW+L+   EL GI LS+SLSMMPA+SVSALVFAHP+S+YFAVG +GKDQV SYAERK  ++   E+WLSPILNY+RD
Sbjct:   82 SYPHFVRGRDSLREYITSLFTSKIAIYDGAMGTMIQNYSKRNKLDEEEYRGDRFKDWNCNVKGNNDMLSITQPHVISGIYKEYLEVGGSNLIGTNTFSSTTIAMADYNMEAYAYELNYEGARLARQACDEVTAADPTNPRFVVGAIGPTNRTGSISPSVEDPSARNVTFDELVEAYFEQIVGLMDGGADILMVETIFDTLNAKAALYAVGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAVHMIPFVEKLAKVVECFLHVYSNAGLPNAMGGYDESPDDMARNNKTFFENGWLNMVGGCCGSTPPHIKA----IRECA---ENYAPRKLPDVGRPKMWLSGLEDLVVDDVHNHLGLPFLNVGERCNISGSIRFKKLMMAGDYATAMDIAKKQVEDGAHVLDINVDDGMLDGLAAMQKFVKIAVTEPEVSKVPFMLDASKFEIVLAGLKWCQGKCIVNSISLKVGEELFKEQATLLKKHGAAVVVMAFDEEGQAATEDEKVRICKRSYDILVNEVHFPPEDIIFDPNVLTIGTGMEEHANYGVDFINAVKTIKEQCPYVKISGGISNLSFGFRGVMKIRESIHAVFLHHAILESGMDVGIVNANEMLHVTEVEGELKKACENLVFNKTPDATDAMLELTQKEKRAIEARKKGGAVTEIKEKSWRDFEAKKRLEHSLVNGISQYIDKDVEEARLM----SSKPLDVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKKQALIDAGLDPEEFDPEDDSAYAGKVLMATVKGDVHDIGKNIVAVVLGCNNYK-------VYDIGVMVSCETILEKAKEYNVDVIGLSGLITPSLDEMVVVAKEMSKAGFKQPLLIGGATTSKMHTAVKLSPNYFTLEHPVIHVLDASRSVTVVSSLLGENKEDFVTDIMEEYDEMREDYYAGLEDRYFLTFEQAKEQKFKIDFDAKSPAPAPNKVGITVVD-YVELENVVPYIDWNPFFQTWELRGRYPNRGYPKIFDDEAVGNEARKLFDDAQAMLKEIIAEKSMHLKGVVGLFPANQSEDGEDVHIYSDEDSRAAGNPTATFCMLRQQAEKENEDPYLSQADFIAP--AGYKDHLGMFAVSCFGCEALVEKYEAEHDDYSKIMSQALADRFVEAFAEYLHKVIRTEMWGYANNEELNEADLLKIKYDGIRPAPGYPSQPDHTEKTTMWNLIKAKELAGISLSDSLSMMPAASVSALVFAHPDSKYFAVGQVGKDQVESYAERKKFELPVAERWLSPILNYERD 1362          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A7S4J684_9STRA (Methionine synthase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4J684_9STRA)

HSP 1 Score: 1688 bits (4371), Expect = 0.000e+0
Identity = 868/1300 (66.77%), Postives = 1038/1300 (79.85%), Query Frame = 2

Query:  155 NYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKY----KLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLE-SGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSP---PREPFMWLSGLEDLVVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAV-KAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKE--KALKNKGKAT-VETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPA-ATVDGEDVEVYANESREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            ++P+F RG+DS   Y+ +LF  RI  +DGAMGTMIQ +    KL EEEYRGE FKD+   VKGNNDMLS+T+P +I  I+ +YLE  GSD+IGTNTFSSTTIAMADYEME   YELN+E ARLAR AC E TA DPS+PRFV+G++GPTNRTGSISP VEDPS RNVTFDELV  Y +Q  GL+DGG DI++VETIFDTLNAKAA++AV EY +  GL D+P+FVSGTLVDQSGRTLSGQT E FY S+RH KP C+GLNCALGA  M PF+++LAK  ECF+HVYSNAGLPNAMGGYD+TPE MA  N +F E+G +NM+GGCCGSTPPHIK       ECA + S  P      P MWLSGLEDLVV          FLNVGERCN++GSI+FKKL++KGDY +AMD+AK QVEDGA VIDIN+DDG+LDGLAAMQKF KIAVTEP+V KVPFM+D+SKFEIV AGLKW QGK IVNSISLKVGE  F+  A ++K HGAAVVVMAFDE+GQAA+ D KVRICKRSYD+LV+EVGFPPEDIIFDPN+LTI TG++EHN+Y +DFI AT+RIK  CPY KISGG+SNLSFGFRGV  IRE++H+VFL+HA+ ++GMD+GIVNA  ++   ++EP L    E+ V NK   +T+ +LD  + EKE  +A K  G A  V+ + WR  +   RL HSL+ GI ++V++DVEE R   D    +PLDVIEGPLM GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFM+ EK+  MIA G++P   + +D+S +AGKVL+ATVKGDVHDIGKNIV+VVLGCNNYK       VYD+GVM  C+KIL KA E  VD++GLSGLITPSLDEMV VAKEMSKAG K PLLIGGATTSKMHTAVK+SP Y T E PVIHVLDASRSVTVV +LL   K D+V ++MEEY+E+RE++YAG E+R++LTF++A+ ++  IDF+  PPAPAP + G+TV+    +L+D++ YIDWNPFFQTWELRGRYPNRGYPKIF DEAVG+EAKKLF+DA AM+   + EK++ L GVVG+FPA  + DGEDV VY +E+        A FCMLRQQAEKE+DDPYLSQADF+AP  +G  D++GMFAV+C GC+ +V +FE  NDDYSKIM QAL DRFVEAFAE LH  IR ++WGYAPDE+++  DL K+KY GIRPAPGYPSQPDHTEKRTMWD++   EL GI+LSESLSMMPA+SVSALVFAHP+S+YFAVG IGKDQV SY  RK  +++  E+WLSPILNY+RD
Sbjct:  138 SFPHFVRGRDSAREYITKLFTSRIAFYDGAMGTMIQNFSKKNKLEEEEYRGEHFKDWKCNVKGNNDMLSMTRPDVISGIYKQYLEIGGSDMIGTNTFSSTTIAMADYEMEDYAYELNYEGARLARLACDEVTAADPSRPRFVVGAIGPTNRTGSISPSVEDPSVRNVTFDELVETYFEQAVGLVDGGSDILMVETIFDTLNAKAALYAVGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAQHMVPFVEKLAKCVECFMHVYSNAGLPNAMGGYDETPEMMAEYNKVFFEKGWLNMVGGCCGSTPPHIKA----IRECAQNFSPRPLPAVGRPKMWLSGLEDLVVDDVHNHLGLPFLNVGERCNIAGSIKFKKLMMKGDYGSAMDIAKAQVEDGAHVIDINVDDGMLDGLAAMQKFVKIAVTEPEVCKVPFMLDASKFEIVMAGLKWCQGKPIVNSISLKVGEKLFKEQATLLKKHGAAVVVMAFDENGQAAEEDEKVRICKRSYDMLVDEVGFPPEDIIFDPNVLTIGTGMEEHNDYGIDFINATKRIKEQCPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAILESGMDVGIVNAKEMLALSEVEPVLKTACENLVFNKTADSTDVMLDLTKKEKEAIEARKRGGGAVEVKKTSWRDLEATKRLEHSLVNGISEFVVKDVEEARQKCD----KPLDVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMDKEKKEAMIAKGLNPDDFDENDESNFAGKVLMATVKGDVHDIGKNIVSVVLGCNNYK-------VYDIGVMVSCEKILEKAKEYDVDVIGLSGLITPSLDEMVVVAKEMSKAGFKQPLLIGGATTSKMHTAVKLSPNYFTLEHPVIHVLDASRSVTVVSSLLGRDKGDFVTDIMEEYDEMREDYYAGLEDRYFLTFDQAKEQKCVIDFESKPPAPAPKKLGITVIDS-VSLEDIVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGKEAKKLFDDAIAMMNTFISEKSMHLKGVVGLFPANRSEDGEDVHVYLDEAARAAGTPEATFCMLRQQAEKENDDPYLSQADFVAP--AGYKDHLGMFAVSCFGCDDLVNKFEAENDDYSKIMSQALADRFVEAFAEYLHRGIRTEMWGYAPDEELDEADLLKIKYDGIRPAPGYPSQPDHTEKRTMWDVIKAEELAGIQLSESLSMMPAASVSALVFAHPDSQYFAVGQIGKDQVESYGARKKFELSVMERWLSPILNYERD 1418          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: A0A7S3JTL8_9STRA (Methionine synthase n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JTL8_9STRA)

HSP 1 Score: 1688 bits (4371), Expect = 0.000e+0
Identity = 868/1306 (66.46%), Postives = 1030/1306 (78.87%), Query Frame = 2

Query:  134 EPKGSVAN-YPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQKYKLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYL-ESGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPPRE-PFMWLSGLEDLVVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNE-VGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHA-VKAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEKALKNK----GKATVETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLS-DRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPAATVDG-EDVEVY----ANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            +PKG     +PYF RG+DS   YL +LFQ RI M+DGAMGTMIQK+KL EE++RGERFKDYH+L+KGNND+LSIT+P II  I+ KYL E GS +IGTNTFSSTTIA ADY+M+  +YELN++SARLARE C + TA+DP+KPRFV+G++GPTNRT SISPDV DP+ RN TFDELV AY +Q  GL+DGG DI+IVETIFDTLNAKAAVFAV EY +   + D+PL +SGTLVDQSGRTLSGQT E FY S+RH KP C+GLNCALGA+ M PFL+RLA  AECFVHVYSNAGLPNAMGGYDDTP+DMAR+NL+FAE G +NM+GGCCGSTPPHI        +C   P   P R+ P MWLSGLED VV          F+NVGERCN++GS +FK+L+++G +  AMD+AK QVE+GA ++D+N+DDG+LDG+ AMQKF K+AVTEPDV+KVPFMIDSSKFEIVEAGLKW QGK IVNSISLKVGE+ F+ HA  ++ HGAAVVVMAFDE GQAA  ++KVRICKRSYDILV+E + +PPEDI+FDPNILTI TGI+EHNNY VDFI A   IK +CP+ KISGGVSNLSFGFRGVN+IRE++H+VFL+HA + +GMDMGIVNA  ++   ++   L  +  DAV N  P ATE LL A E EKE A   K      A  +T+ WR   +  R+THSL+KGI ++V +D EE R  + A G RPL+VIEGPLM+GMNVVGDLFG+GKMFLPQVIKSARVMKKAVA+LLP+MEAEK   +IA GIDP S   DDDSQYAGK LIATVKGDVHDIGKNIVAVVLGCNNYK       VYD+GVM  CDKIL KA E KVD +GLSGLITPSLDEMVFVAKEMSK G ++PLLIGGATTSKMHTAVKI+PQYA+ E PVIHVLDASR+VTVV +LL+ D K DYV ++ +EY ELR+E+YA  E+R +LT E+AQ KR ++++ K PPAP       T+    Y L DV+  IDWNPFFQTWELRGRYPNRGYPKIF DE VG EAK+LF+DAQ M+K+I++EK L L GV G++ A   +G EDV+V+    A +++E+  A FCM RQQAE ES   Y+SQADFIAP+     DY+GMFAVAC G E+   ++E  NDD+SKIM+QAL DR VEA AE LH D+R K WGYA DE +  +DL K+KY+GIRPAPGYPSQPDHTEKRTMWDLL+V   +GI+LSES+SMMPASSVSALVFAHPE+ YFAVG I KDQV SYAERKG  +   E+WLSP+LNYDRD
Sbjct:   76 QPKGLYPEGFPYFVRGRDSVREYLTKLFQTRITMYDGAMGTMIQKHKLEEEDFRGERFKDYHMLIKGNNDLLSITKPDIIGGIYEKYLVEGGSQLIGTNTFSSTTIAQADYDMQSLIYELNYDSARLAREVCDKVTAQDPTKPRFVVGAIGPTNRTASISPDVNDPTARNCTFDELVEAYYEQCVGLVDGGADILIVETIFDTLNAKAAVFAVNEYLEHANV-DIPLIISGTLVDQSGRTLSGQTGEAFYASIRHAKPICVGLNCALGASAMAPFLKRLADCAECFVHVYSNAGLPNAMGGYDDTPQDMARENLMFAENGWVNMIGGCCGSTPPHIAAIQEAIKDCK--PRPLPTRKAPRMWLSGLEDFVVESAHNNVGLPFVNVGERCNIAGSRKFKRLVMEGKFAEAMDIAKDQVENGAHIVDVNVDDGMLDGMNAMQKFIKMAVTEPDVSKVPFMIDSSKFEIVEAGLKWCQGKPIVNSISLKVGEEKFKEHALCLRKHGAAVVVMAFDEQGQAASREDKVRICKRSYDILVSEPIYYPPEDIVFDPNILTIGTGIEEHNNYGVDFIAAVREIKQVCPWAKISGGVSNLSFGFRGVNIIRESIHAVFLHHACLDSGMDMGIVNAKEMLALSEVPKHLRGVCIDAVMNTRPDATERLLTATEAEKEAAEARKRGGGDVAVAKTTTWRDMPIEERITHSLVKGISEFVDKDTEELRAEIAARGGRPLEVIEGPLMAGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPYMEAEKRENLIAQGIDPDSQQQDDDSQYAGKFLIATVKGDVHDIGKNIVAVVLGCNNYK-------VYDIGVMCECDKILEKAKEYKVDAIGLSGLITPSLDEMVFVAKEMSKKGLQVPLLIGGATTSKMHTAVKIAPQYASDEHPVIHVLDASRAVTVVSSLLNADDKPDYVADIADEYAELRDEYYASLEDRKFLTLEQAQAKRCELNWSKVPPAPKTALGAHTIT---YELSDVVPLIDWNPFFQTWELRGRYPNRGYPKIFNDETVGAEAKRLFDDAQKMMKQIVDEKRLELRGVYGLYAAQRSEGGEDVDVWDSDAARDAKEKPKATFCMFRQQAETESAAAYMSQADFIAPR-GAAKDYLGMFAVACFGAEEWANKYEAQNDDFSKIMMQALADRLVEACAEALHRDMRTKYWGYASDETLSAEDLIKVKYEGIRPAPGYPSQPDHTEKRTMWDLLDVESRSGIKLSESISMMPASSVSALVFAHPEATYFAVGKIEKDQVKSYAERKGYSLPEMERWLSPMLNYDRD 1367          

BLAST of mRNA_P-fluviatile_contig18.3170.1 vs. uniprot
Match: B7GBG7_PHATC (Methionine synthase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7GBG7_PHATC)

HSP 1 Score: 1686 bits (4366), Expect = 0.000e+0
Identity = 867/1267 (68.43%), Postives = 1024/1267 (80.82%), Query Frame = 2

Query:  245 MGTMIQKY----KLGEEEYRGERFKDYHILVKGNNDMLSITQPHIIKEIHTKYLESG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARLAREACAEATAKDPSKPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVIAYKDQTKGLIDGGVDIIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCLGLNCALGATQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPEDMARDNLLFAEEGLINMLGGCCGSTPPHIKVRAIHTSECALHPSSSPP-REPFMWLSGLEDLVVTKERFQ----FLNVGERCNVSGSIRFKKLILKGDYQAAMDVAKQQVEDGAMVIDINLDDGLLDGLAAMQKFCKIAVTEPDVAKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEDDFRRHARVVKAHGAAVVVMAFDEDGQAADCDNKVRICKRSYDILVNEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKALCPYCKISGGVSNLSFGFRGVNVIREAMHSVFLYHAV-KAGMDMGIVNAGMLVVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEKEKALKNKGKAT--VETSGWRTQDVNARLTHSLIKGIDQWVIEDVEECRLMLDAEGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKEAKMIADGIDPSSVNPDDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASKEANGVYDMGVMQPCDKILAKAIELKVDIVGLSGLITPSLDEMVFVAKEMSKAGCKLPLLIGGATTSKMHTAVKISPQYATPEAPVIHVLDASRSVTVVGALLSDRKADYVEELMEEYEELREEHYAGQEERHYLTFEKAQTKRKKIDFQKTPPAPAPLQPGVTVVTGKYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFMDEAVGEEAKKLFNDAQAMLKRILEEKTLSLTGVVGIFPA-ATVDGEDVEVYANESREE---VLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAVACMGCEKMVKEFENLNDDYSKIMVQALTDRFVEAFAEKLHEDIRKKIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNVAELTGIELSESLSMMPASSVSALVFAHPESEYFAVGTIGKDQVSSYAERKGIDVATCEKWLSPILNYDRD 3994
            MGTMIQ Y    +L EEEYRGERF+D++  VKGNNDMLSI+QPHIIK I+ +YLE G S++IGTNTFSSTTIAMADY+ME   YELN+  ARLAREAC E TAKDPSKPRFV+G++GPTNRT SISP VEDP+ RNVTFDELV  Y +Q  GL+DGG D+++VETIFDTLNAKAA++A+ E+ +  GL D+P+FVSGTLVDQSGRTLSGQT E FYVS+RH KP C+GLNCALGA  M PF++RL+K AECFVHVYSNAGLPNAMGGYDDTPEDMAR+N +F E G +NM+GGCCGSTPPHIK  AI  +     P   P    P MWLSGLEDL V     Q    FLNVGERCN++GSI+FKKL++KGDY AAMD+AKQQVEDGA VIDIN+DDG+LDGLAAMQKF KIAVTEP++AKVPFM+D+SKF+IV AGLKW QGK IVNSISLKVGE+ F   A ++K HGAAVVVMAFDE GQAA  D K+RICKRSYD+LVN+VGFPPEDI+FDPN+LTI TG++EH NY VDFI + +RIK  CPY KISGG+SNLSFGFRGV  IRE++H+VFL+HA+ ++GMD+GIVN+  L+  DD+EP +  + E+ V NK P AT+ +L+    E+     +KG     V+ S WR  + + RL HSL+ GI ++V  DVEE R++      +PLDVIEGPLM GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFM+AEK   MIA G+DP  V+PDDDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNNYK       VYD+GVM  C+KILAKA E  VD++GLSGLITPSLDEMV VAKEMSK G K PLLIGGATTSKMHT+VKI+P Y TPE P IHVLDASRSVTVV +LL + K ++VE++MEEYEE+RE++YAG E+R++LTFEKA+ ++  I+F ++P A  P + G+TV+     +  V+ YIDWNPFFQTWELRGRYPNRGYPKIF DEAVG EAKKLF+DAQ ML +I E+ +++L GVVGIFPA  + DGEDV VY  E+  E   V   FCMLRQQAEKESDDPYLSQADF+AP  +G  D++GMFAV+C GC+ +VK+FE+ NDDYSKIM QAL DRFVEAFAE +H ++R  +WGYA  E++   DL K+KY GIRPAPGYPSQPDHTEKRTMW+LL   EL GIELSESLSMMPASSVSALVFAHPESEYFAVG +GKDQV++YA RK +D+A CE+WLSPILNY+RD
Sbjct:    1 MGTMIQNYAKKNRLDEEEYRGERFRDWNCAVKGNNDMLSISQPHIIKGIYMQYLEEGGSNMIGTNTFSSTTIAMADYQMEAYAYELNYVGARLAREACDEVTAKDPSKPRFVVGAIGPTNRTCSISPSVEDPAARNVTFDELVETYLEQVVGLVDGGADVLMVETIFDTLNAKAALYAIGEFLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYVSIRHAKPMCVGLNCALGAKHMVPFVERLSKAAECFVHVYSNAGLPNAMGGYDDTPEDMARENEVFFENGWLNMVGGCCGSTPPHIK--AIRETAERYQPRKLPDVGRPKMWLSGLEDLKVEDVHNQLGLPFLNVGERCNIAGSIKFKKLMMKGDYGAAMDIAKQQVEDGAHVIDINVDDGMLDGLAAMQKFVKIAVTEPEIAKVPFMLDASKFDIVMAGLKWCQGKCIVNSISLKVGEELFMEQATLLKKHGAAVVVMAFDEQGQAATEDEKIRICKRSYDVLVNKVGFPPEDIVFDPNVLTIGTGMEEHANYGVDFINSVKRIKEECPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAITESGMDVGIVNSHELLSVDDLEPDMKLLCENLVFNKTPDATDDMLERTNFER-----SKGGVVDEVKKSSWRDLEPSKRLEHSLVNGISEFVDSDVEEARVL----ATKPLDVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMDAEKRENMIAAGLDPDDVDPDDDSNYAGKVLMATVKGDVHDIGKNIVAVVLGCNNYK-------VYDIGVMCSCEKILAKAKEYNVDVIGLSGLITPSLDEMVDVAKEMSKQGFKQPLLIGGATTSKMHTSVKIAPSYTTPEHPTIHVLDASRSVTVVNSLLGENKGEFVEDIMEEYEEMREDYYAGLEDRNFLTFEKAKEQKITINFDQSPVADTPNKIGITVID-TVKISAVVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGGEAKKLFHDAQTMLNQICEDGSMTLKGVVGIFPANRSEDGEDVHVYETEADRESGNVSTTFCMLRQQAEKESDDPYLSQADFVAP--AGYKDHLGMFAVSCFGCDALVKKFESENDDYSKIMAQALADRFVEAFAEYVHREMRLDLWGYAAGEQLNESDLLKVKYDGIRPAPGYPSQPDHTEKRTMWNLLQAQELAGIELSESLSMMPASSVSALVFAHPESEYFAVGQVGKDQVTNYASRKQMDLALCERWLSPILNYERD 1245