mRNA_P-fluviatile_contig14.2200.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: D7FNM0_ECTSI (Tetrahydrofolate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNM0_ECTSI)

HSP 1 Score: 682 bits (1759), Expect = 5.670e-231
Identity = 405/714 (56.72%), Postives = 456/714 (63.87%), Query Frame = 2

Query:  488 QLDISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTA--------------------------------------------TATAEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVL----RECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETRKKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTARAAETKGKDDDGALPLTADSATTVEAAALPPVDRRPRPRTQPALQV---------RRSRRILWFNASHERDVFPLLDKLASARRPAPLSKSDGYGGDEDE-------NDKWPLFDEAWFMELNPGRPSRFAIPSAEEILAHHGVSPAAADAVVGRIMDGAAAAVGDVQPSPVGRCLPGMSEGPAEASTCPGSAGAESGEYQNAAGGRREG---EKEKEAGVEARAGAWQRTLQEVFTCLQARRGGRLSTTLTGKASEFMRKLGLICTNGQNKA---AATEPVDRGTHRSGGTSALTAD---SDHKNEWEAVEAGQPGKGSSGDV-VVEILCTGSLYAVAAALESFGAQV 2407
            QLD+SGLSVIHVTGTKGKGSTCAMVES+LR HG+RTGLYTSPHLLEVTER+R DGKPL+KE FA YFF+VWDRLQ TA                                            T   EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGR+DATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGC AM VEQPRGGMSVL    ++CA +AGCGELEVV+PLPT DP GRAYEIGLAG+HQRVNA LALRLCAVW+E  ++Q      QQQ+    +AP    I DT+ F  SA+VH+GLS C WPGRCQVFR PAFPSVT+YLDGAHTEQSM ACL WFV+KTA AA ++ K D+ A      S+    A A+ P+D    PR+ P             RR RR+LWFNASHERDV PLLD LASARR      S G              +   PLFDEAWFME+ PGRPSRF +P+A+EILA HG+SPA A          AAAA                S+ P             SGE    AGG   G   EK   AG  + AGAWQRTL++V+T LQ RRGG LS TLTG A  F  KL LIC N Q+     AA+   D G       SA+ AD   S  + +      G   +G   D  +VE+LCTGSLYAVAAALE+FGA+V
Sbjct:   57 QLDLSGLSVIHVTGTKGKGSTCAMVESILRHHGFRTGLYTSPHLLEVTERVRCDGKPLTKEAFARYFFQVWDRLQDTAAPTNTDDATAAATXXXXGTTGITAAASEGPSQAEQGPAAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLCEASQQCAAAAGCGELEVVKPLPTRDPCGRAYEIGLAGDHQRVNAALALRLCAVWIEAHRQQ------QQQQHAGDQAPPAEKIHDTETFWASADVHRGLSCCVWPGRCQVFRPPAFPSVTIYLDGAHTEQSMRACLGWFVEKTATAAASRAKADEVA------SSRAAVAVAVTPLDI---PRSSPXXXXXXXXXXXXNRRCRRVLWFNASHERDVVPLLDMLASARRRESCDGSSGNAXXXXXXXXXXXADGDTPLFDEAWFMEVIPGRPSRFKLPTAQEILAPHGISPAVA----------AAAATSXXXXXXXXXXXGPCSDDP-------------SGEEP--AGGAAPGCGEEKADGAGAXSGAGAWQRTLEQVWTSLQTRRGGTLSATLTGTAPFFFEKLSLICANSQHDGGSEAASVVGDPGVASRTAVSAVDADHHGSGGRGDASVAATGCGDRGGRVDSEIVEVLCTGSLYAVAAALEAFGAEV 730          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: A0A6H5L5N3_9PHAE (Tetrahydrofolate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L5N3_9PHAE)

HSP 1 Score: 537 bits (1384), Expect = 1.640e-177
Identity = 320/574 (55.75%), Postives = 355/574 (61.85%), Query Frame = 2

Query:  557 MVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQ----------STATATA---------------------------------EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRE----CAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETRKKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTARAAETKGKDDDGALPLTADSATTVEAAALPPVDRRPRPRTQPAL---------QVRRSRRILWFNASHERDVFPLLDKLASARRPAPLSKSDGY-------GGDEDENDKWPLFDEAWFMELNPGRPSRFAIPSAEEILAHHGVSPAAADAVVGRIMDGAAAAVGDVQPSPVGRCLPGMSEGPAEASTCPGSAGAESGEYQNAAGGRREGEKEKEAGVEARAGAWQRTLQEVF 2089
            MVESVLR HG+RTGLYTSPHLLEVTER+R DGKPL+KE FA YFF+VWDRLQ          +TA A A                                 EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGR+DATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGC AM VEQPRGGMSVLRE    CA +AGC ELEVV+PLP  DP GRAYEIGLAG+HQRVNA LALRLC VW+E  ++Q        Q      AP    I +T+AF  SAEVH+GLS C WPGRCQVFR PAFPSVT++LDGAHTEQSM ACL WFV+K    A ++                   AA    V  R  PR+ P           Q RR RR+LWFNASHERDV PLLD LASARR    + S            D+D +   PLF+EAWFME+N GRPSRF +P+A+EILA HG+ PA A A        AA   G    S VG    G S          G A    GE           EK   AG  + AGAWQRTL++ +
Sbjct:    1 MVESVLRHHGFRTGLYTSPHLLEVTERVRCDGKPLTKEAFARYFFQVWDRLQDPAARTNTDDATAAAVAATGTTGITAAASEGPSQAKQGPTAPEEEGTTAPEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRFDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCRAMAVEQPRGGMSVLREASPKCAAAAGCSELEVVKPLPARDPCGRAYEIGLAGDHQRVNAALALRLCDVWIEAHRQQXXXXXAGDQ------APPPEKIRETEAFWASAEVHRGLSCCVWPGRCQVFRPPAFPSVTIHLDGAHTEQSMRACLGWFVEKGDEVASSR-------------------AAVAVAVTPRDIPRSSPXXXXXQLELRRQKRRCRRVLWFNASHERDVVPLLDMLASARRREARNGSXXXXXXXXXXADDDDADGDTPLFEEAWFMEVNTGRPSRFKLPTAQEILASHGMFPAVAAA--------AATPPGMAGASGVGS---GPSSDDPXXXXXXGGAAPGCGE-----------EKADGAGAXSGAGAWQRTLEQGY 527          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: A0A0S7K0M8_9TELE (Tetrahydrofolate synthase (Fragment) n=2 Tax=Poeciliopsis prolifica TaxID=188132 RepID=A0A0S7K0M8_9TELE)

HSP 1 Score: 292 bits (747), Expect = 7.970e-86
Identity = 161/349 (46.13%), Postives = 213/349 (61.03%), Query Frame = 2

Query:  488 QLDISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTATATA-EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETR--KKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTARAAETKGKDDDGALPLTADSATTVEAAAL 1525
            QLD   L++IHVTGTKGKGSTCA  E +LR +G+RTG Y+SPHL++V ERIR DG+P+S E F  YF++V+ RL  T  A    MP YF  LT++    F+QE VD+ ++EVG+GG YD TNVV  P  CGV+ L +DHTQ+LGDT++KIAW+K GIFK G  A TV QP+  M+VLR+ A+   C  L V   L        A ++GLAG+HQR NA LAL+LC  W++ R     +  L            P    +   D+FR  A + KGL++  W GR Q  R  A   +T +LDGAHT +SM AC++WF +  A A    G     A  L  +S+   +AAA+
Sbjct:   14 QLDH--LNIIHVTGTKGKGSTCAFTEQILRTYGFRTGFYSSPHLVQVRERIRIDGRPISNELFTKYFWQVYGRLDKTKEAHGGTMPAYFRFLTILAFHVFLQEKVDLAIIEVGIGGAYDCTNVVRRPWVCGVSSLGIDHTQILGDTIEKIAWQKAGIFKPGVPAFTVRQPQEAMAVLRDRAREIRC-PLRVCPDLDRYQTESGALQLGLAGQHQRSNASLALQLCHTWLQRRCGPDSSPALP----------DPDSSCLSQADSFRPGAAMVKGLADTHWAGRNQTLRRGA---LTYFLDGAHTHRSMQACVQWFQE--AAAQPQSGASGAVARVLLFNSSGQRDAAAM 344          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: A0A8C4KQ54_DRONO (Folylpolyglutamate synthase n=1 Tax=Dromaius novaehollandiae TaxID=8790 RepID=A0A8C4KQ54_DRONO)

HSP 1 Score: 293 bits (750), Expect = 2.390e-84
Identity = 155/328 (47.26%), Postives = 207/328 (63.11%), Query Frame = 2

Query:  494 DISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTAT-ATAEMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETRKKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTARAAETKGKDDDG 1474
            D+  L++IHVTGTKGKGS CA  E +LR +G +TG Y+SPHL++V ERIR +G+P+SKE F+ YF+ V++RL+ T   A A MP YF  LT++    F+QE VD+ V+EVG+GG YD TN++  PV CGV+ L +DHT +LGDTL+KIAW+KGGIFK G  A TV QP   + VLR+ A+   C  L +   L   +  GRA  +GLAG+HQR NA LAL+L   W++ R  + +             +P    +    AFR S  + +GL E  WPGR QV   P  P VT Y+DGAHT  S+ AC++WF     +AA  + K  DG
Sbjct:   63 DLDRLNIIHVTGTKGKGSACAFTERILRGYGLKTGFYSSPHLVQVRERIRINGQPISKELFSKYFWLVYNRLEETKDPARASMPAYFRFLTIMAFHVFLQEQVDLAVVEVGIGGAYDCTNIIRKPVVCGVSSLGIDHTSILGDTLEKIAWQKGGIFKPGVPAFTVAQPERPLEVLRDRARERAC-PLYLCPELDAFEEGGRALALGLAGDHQRSNAALALQLARAWLQRRGYEGKTP-----------SPXGRPVPLASAFRPSVAMVQGLREAEWPGRTQVL--PHGP-VTWYIDGAHTTSSVQACVRWF----RQAALNENKPQDG 371          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: UPI0005CC571D (folylpolyglutamate synthase, mitochondrial isoform X2 n=2 Tax=Oryzias latipes TaxID=8090 RepID=UPI0005CC571D)

HSP 1 Score: 292 bits (747), Expect = 9.670e-84
Identity = 151/317 (47.63%), Postives = 200/317 (63.09%), Query Frame = 2

Query:  494 DISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTATATA-EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETRKKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTAR 1441
            +++ L++IHVTGTKGKGSTCA  E +LR +G RTG Y+SPHL++V ERIR +G+P+ K+ F  YF++++ RL+ +  A+   MP YF  LT++    F+QE VDV V+EVG+GG YD TN++  P  CGVA L +DHTQ+LGDT++KIAW+KGGIFK G  A TV QP G M+VLR+ AK   C  L V   L      G A  +GLAG+HQR NA LAL+L   W++ R    Q L            P    +    AFR S+E+  GL+   WPGR Q  +      VT +LDGAHT +SM AC+ WF    A+
Sbjct:   95 ELNRLNIIHVTGTKGKGSTCAFTEKILRSYGLRTGFYSSPHLVQVRERIRINGQPIGKDIFTKYFWQIYGRLEESKPASGGTMPAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQPEGAMAVLRDRAKEIQC-PLWVCPNLDEYQTDGTALHLGLAGKHQRWNASLALQLSNSWLKRRCSPDQSLS--------AINPENTRVQPATAFRPSSEMMMGLANTEWPGRNQTLKNG---EVTYFLDGAHTVRSMQACVDWFKGSAAQ 399          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: UPI0007E9809E (folylpolyglutamate synthase, mitochondrial isoform X3 n=1 Tax=Poecilia reticulata TaxID=8081 RepID=UPI0007E9809E)

HSP 1 Score: 289 bits (739), Expect = 1.400e-83
Identity = 152/322 (47.20%), Postives = 201/322 (62.42%), Query Frame = 2

Query:  488 QLDISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTATATA-EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETR--KKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTARA 1444
            QLD   L++IHVTGTKGKGSTCA  E +LR +G+RTG Y+SPHL++V ERIR DG+P+  E F  YF++V+ RL  T  A    MP YF  LT++    F+QE VD+ ++EVG+GG YD TNVV  P  CG+  L +DHTQ+LGDT++KIAW+K GIFK G  A TV QP+  M+VLR+ A+   C  L V   L        A ++GLAG+HQR NA LAL+LC  W++ R     +  L            P    +   D+FR  A + KGL+E  W GR Q  R  A   +T +LDGAHT +SM AC++WF +  A++
Sbjct:   95 QLDH--LNIIHVTGTKGKGSTCAFTERILRTYGFRTGFYSSPHLVQVRERIRIDGRPIGNELFTKYFWQVYGRLDETKEAHGGTMPAYFRFLTILAFHVFLQEKVDLAIIEVGIGGAYDCTNVVRRPWVCGITSLGIDHTQILGDTIEKIAWQKAGIFKPGVPAFTVRQPQEAMTVLRDRAREIQC-PLRVCPDLDRYQTESGALQLGLAGQHQRSNASLALQLCHTWLQRRCGPDLSPALP----------DPDTSCVLQADSFRPGATMVKGLAEARWAGRNQTLRRGA---LTYFLDGAHTHRSMQACVQWFQEAAAQS 400          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: A0A3P9NNL6_POERE (Folylpolyglutamate synthase n=2 Tax=Poecilia TaxID=8080 RepID=A0A3P9NNL6_POERE)

HSP 1 Score: 290 bits (741), Expect = 2.250e-83
Identity = 152/328 (46.34%), Postives = 203/328 (61.89%), Query Frame = 2

Query:  470 LSHNAGQLDISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTATATA-EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETR--KKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTARA 1444
            + +   QLD   L++IHVTGTKGKGSTCA  E +LR +G+RTG Y+SPHL++V ERIR DG+P+  E F  YF++V+ RL  T  A    MP YF  LT++    F+QE VD+ ++EVG+GG YD TNVV  P  CG+  L +DHTQ+LGDT++KIAW+K GIFK G  A TV QP+  M+VLR+ A+   C  L V   L        A ++GLAG+HQR NA LAL+LC  W++ R     +  L            P    +   D+FR  A + KGL+E  W GR Q  R  A   +T +LDGAHT +SM AC++WF +  A++
Sbjct:   46 MEYQVEQLDH--LNIIHVTGTKGKGSTCAFTERILRTYGFRTGFYSSPHLVQVRERIRIDGRPIGNELFTKYFWQVYGRLDETKEAHGGTMPAYFRFLTILAFHVFLQEKVDLAIIEVGIGGAYDCTNVVRRPWVCGITSLGIDHTQILGDTIEKIAWQKAGIFKPGVPAFTVRQPQEAMTVLRDRAREIQC-PLRVCPDLDRYQTESGALQLGLAGQHQRSNASLALQLCHTWLQRRCGPDLSPALP----------DPDTSCVLQADSFRPGATMVKGLAEARWAGRNQTLRRGA---LTYFLDGAHTHRSMQACVQWFQEAAAQS 357          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: F1Q7N0_DANRE (Folylpolyglutamate synthase n=16 Tax=Cyprinoidei TaxID=30727 RepID=F1Q7N0_DANRE)

HSP 1 Score: 290 bits (741), Expect = 6.310e-83
Identity = 148/311 (47.59%), Postives = 198/311 (63.67%), Query Frame = 2

Query:  494 DISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTATATA-EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETRKKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWF 1423
            ++  L++IHVTGTKGKGSTCA  E +LR +G+RTG Y+SPHL++V ERIR +G+P+ KE F  YF++V+ RL+ T  A    MP YF  LT++    F+QE VD+ V+EVG+GG YD TN++  P  CG++ L +DHT +LGDT++KIAW+KGGIFK G  A TV+QP G M VL+E A+  GC  L V   L   +  G +  +GLAG+HQR NA LAL+L   W+     Q  IL              F  +    AF+ S  + KGL+E  WPGR Q  +      VT Y+DGAHT +SM AC++WF
Sbjct:   94 ELDRLNIIHVTGTKGKGSTCAFTEKILRNYGFRTGFYSSPHLVQVRERIRINGQPIGKELFTKYFWQVYGRLEETKDAHGGSMPAYFRFLTILAFHVFLQEKVDLAVIEVGIGGAYDCTNIIRRPWVCGISSLGIDHTSILGDTIEKIAWQKGGIFKPGVPAFTVKQPDGPMKVLQERAEEVGCS-LSVCPDLDEYESEG-SLSLGLAGQHQRSNASLALQLSHTWL-----QRHILT------DPASPVEFSGVSSAPAFQPSPAMIKGLAETEWPGRNQTLK---HGPVTYYMDGAHTTRSMQACVRWF 388          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: A0A3P9NNJ7_POERE (Folylpolyglutamate synthase n=13 Tax=Poeciliinae TaxID=586240 RepID=A0A3P9NNJ7_POERE)

HSP 1 Score: 289 bits (739), Expect = 1.340e-82
Identity = 152/322 (47.20%), Postives = 201/322 (62.42%), Query Frame = 2

Query:  488 QLDISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTATATA-EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETR--KKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTARA 1444
            QLD   L++IHVTGTKGKGSTCA  E +LR +G+RTG Y+SPHL++V ERIR DG+P+  E F  YF++V+ RL  T  A    MP YF  LT++    F+QE VD+ ++EVG+GG YD TNVV  P  CG+  L +DHTQ+LGDT++KIAW+K GIFK G  A TV QP+  M+VLR+ A+   C  L V   L        A ++GLAG+HQR NA LAL+LC  W++ R     +  L            P    +   D+FR  A + KGL+E  W GR Q  R  A   +T +LDGAHT +SM AC++WF +  A++
Sbjct:   95 QLDH--LNIIHVTGTKGKGSTCAFTERILRTYGFRTGFYSSPHLVQVRERIRIDGRPIGNELFTKYFWQVYGRLDETKEAHGGTMPAYFRFLTILAFHVFLQEKVDLAIIEVGIGGAYDCTNVVRRPWVCGITSLGIDHTQILGDTIEKIAWQKAGIFKPGVPAFTVRQPQEAMTVLRDRAREIQC-PLRVCPDLDRYQTESGALQLGLAGQHQRSNASLALQLCHTWLQRRCGPDLSPALP----------DPDTSCVLQADSFRPGATMVKGLAEARWAGRNQTLRRGA---LTYFLDGAHTHRSMQACVQWFQEAAAQS 400          

BLAST of mRNA_P-fluviatile_contig14.2200.1 vs. uniprot
Match: A0A8C7ZZ20_9TELE (Folylpolyglutamate synthase n=7 Tax=Oryzias sinensis TaxID=183150 RepID=A0A8C7ZZ20_9TELE)

HSP 1 Score: 289 bits (740), Expect = 1.350e-82
Identity = 148/318 (46.54%), Postives = 202/318 (63.52%), Query Frame = 2

Query:  494 DISGLSVIHVTGTKGKGSTCAMVESVLRKHGYRTGLYTSPHLLEVTERIRRDGKPLSKETFAGYFFEVWDRLQSTATATA--EMPTYFHLLTLVGLWCFVQEGVDVCVLEVGMGGRYDATNVVPSPVACGVAMLDLDHTQVLGDTLDKIAWEKGGIFKEGCHAMTVEQPRGGMSVLRECAKSAGCGELEVVEPLPTLDPSGRAYEIGLAGEHQRVNAGLALRLCAVWMETRKKQAQILQRQQQKQQQGRAPHFGAIGDTDAFRRSAEVHKGLSECAWPGRCQVFRTPAFPSVTVYLDGAHTEQSMHACLKWFVDKTAR 1441
            +++ L++IHVTGTKGKGSTCA  E +LR +G+RTG Y+SPHL++V ERIR +G+P+ K+ F+ YF++++ RL+ +    +   MP YF  LT++    F+QE VDV V+EVG+GG YD TN++  P  CGVA L +DHTQ+LGDT++KIAW+KGGIFK G  A TV QP G M+VLR+ AK   C  L +   L      G A  +GLAG+HQR NA LAL+L   W++ R                G++   G    + +FR S+E+  GL+   WPGR Q  R      VT +LDGAHT +SM AC+ WF    A+
Sbjct:  113 ELNRLNIIHVTGTKGKGSTCAFTEKILRSYGFRTGFYSSPHLVQVRERIRINGQPIGKDIFSKYFWQIYGRLEESKVDASGGTMPAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQPEGAMAVLRDRAKEIQC-PLWLCPNLDDYQTDGTALHLGLAGKHQRWNASLALQLSNSWLKRRCSP-------------GKSSRMGQGLLSTSFRPSSEMMMGLANTEWPGRNQTLRNG---EVTYFLDGAHTVRSMQACVDWFKGSAAQ 413