mRNA_P-fluviatile_contig14.2076.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: D7FNG4_ECTSI (5-oxoprolinase (ATP-hydrolysing) 5-oxoprolinase (ATP-hydrolysing) n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FNG4_ECTSI)

HSP 1 Score: 2112 bits (5471), Expect = 0.000e+0
Identity = 1134/1423 (79.69%), Postives = 1183/1423 (83.13%), Query Frame = 1

Query:   16 EKTERTFRFSIDRGGTFTDVYAEVPGSKGFRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAGGEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEVGDVAGXXXXXXXXXXXXXX---------RKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWGSNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGDPSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQ----GCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKPDSAASSMDVDDGVVGGAGGVVGDVEDPDRKSRREAEMRLGGSVSGHRLGQEQA 4245
            E   R FRFSIDRGGTFTDVYAEVPG+KGFRVLKLLSEDP NYPDAPREGVRRILEEETGV HPRD PLDTSRIEYIRMGTTVATNALLER+GEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERV+LDK                                          VVQGVTGEGVRVV++PDLAA+RGSLQAVLDQGITSLAVVFLHSYTFA+HE+RVGKLA EMGF H+SLSSEVMPMVK VPRGFTTAADAYLTPVIARYV SFRSGFD+ FDDVKTLFMQSDGGLTPV+SFRGSRAILSGPAGGVVGYAATSYG    XXXXXXXXX  KPAVIGFDMGGTSTDVSRFAG FEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFR+GLFAVGPQSAGAHPGPVCYRKSGHLAVTDANV+LGRV+P+YFPKIFGPGEDE LD+EGPRLAFEEMA ++N LAAA A+  G  A  PA KTADDVAYGFIRVANEAMCRPIRNLTTMK                                                                         VWEKVR LE +AR +L +QGF P DV CQAYLNLRFQGTDTALMTPA+PP HKAPEPGG         +DDAL+SFF QYRREFGF+LEGRAVVVDDVRVRAVGRSPPA RPRV AAP GQES P PVD ASCYWEGLGRV+T VH+LAELKAGHRVEGPAILIQDVSTVVVEPGC ATVSPFGDVAI+VGDVA     XXXXXXXXXX         RKR KRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDS GGLVANAPHLPVHLGAMQEAVRYQV+HWGSNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEG+GD  GPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVT G FDE GVS LL APGE GDP+CSGTRNLQDNLSDLRAQVAANTRGVALLQGL +EYG+DV+SAYMGHIQ+NAEAAVR MLVDFSLQMGLGEVGTV+AED+MDDGTPIRLAVTVDR  RSALFDF GTG EVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQ    GCL PVEIRIP G LLNPSP AAVVGGNVLTSQRVTDVI KCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTW+GCSGVHTHMTNTRITDPEILERRYPVILN+FGLRRGSGGGGAR GG+GV+RSMT RKPLTVSVLSERR+F+PWGLAGG PAERGLN LRFRGGGG  + R+VS+GGKNTVKV+AGDRLTILTPGGGGHG P                      D+EDPDRKSRREAEMRLGGSVSGHRLGQEQA
Sbjct:   13 EINRRPFRFSIDRGGTFTDVYAEVPGAKGFRVLKLLSEDPQNYPDAPREGVRRILEEETGVAHPRDEPLDTSRIEYIRMGTTVATNALLERQGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVILDKNA---------------------------------------VVVQGVTGEGVRVVKEPDLAAVRGSLQAVLDQGITSLAVVFLHSYTFADHERRVGKLAGEMGFTHVSLSSEVMPMVKAVPRGFTTAADAYLTPVIARYVASFRSGFDRGFDDVKTLFMQSDGGLTPVASFRGSRAILSGPAGGVVGYAATSYGDXXXXXXXXXXXXXEKPAVIGFDMGGTSTDVSRFAGVFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRSGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVFLGRVIPRYFPKIFGPGEDEPLDAEGPRLAFEEMAKQVNALAAATASQTG--ASPPAAKTADDVAYGFIRVANEAMCRPIRNLTTMK-------------------------------------------------------------------------VWEKVRVLEESARSDLAKQGFGPEDVRCQAYLNLRFQGTDTALMTPASPPPHKAPEPGGSGSGGEG--EDDALQSFFGQYRREFGFVLEGRAVVVDDVRVRAVGRSPPAHRPRVEAAPPGQESSPVPVDHASCYWEGLGRVETAVHRLAELKAGHRVEGPAILIQDVSTVVVEPGCTATVSPFGDVAIDVGDVAAEGDDXXXXXXXXXXXXXXXXXXXRKRAKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSTGGLVANAPHLPVHLGAMQEAVRYQVRHWGSNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGQGDGTGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTMGVFDEKGVSDLLHAPGEWGDPACSGTRNLQDNLSDLRAQVAANTRGVALLQGLVSEYGIDVVSAYMGHIQDNAEAAVRHMLVDFSLQMGLGEVGTVNAEDFMDDGTPIRLAVTVDRTMRSALFDFEGTGAEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQISDRGCLAPVEIRIPEGCLLNPSPTAAVVGGNVLTSQRVTDVILKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWSGCSGVHTHMTNTRITDPEILERRYPVILNSFGLRRGSGGGGARRGGDGVSRSMTIRKPLTVSVLSERRSFRPWGLAGGQPAERGLNFLRFRGGGGGNSGRSVSLGGKNTVKVNAGDRLTILTPGGGGHGTPXXXXXXXXXXXXXX--XXXXXXDLEDPDRKSRREAEMRLGGSVSGHRLGQEQA 1317          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: W7TYX7_9STRA (5-oxoprolinase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TYX7_9STRA)

HSP 1 Score: 1567 bits (4058), Expect = 0.000e+0
Identity = 863/1343 (64.26%), Postives = 983/1343 (73.19%), Query Frame = 1

Query:   31 TFRFSIDRGGTFTDVYAEVPGSKGFRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDD-VKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHA--GPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAGGEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEP-------GGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEVGDVAGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWG-SNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGDPSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVK 4026
            +F F+IDRGGTFTDV+  +PG+ GFRVLKLLSEDP NYPDAPREG+RRILE+ETGVPHPRD PLDTSRI  I+MGTTVATNALLER+GE  AL IT GFRDLL+IGNQSRP+IFDL++R+PE+LY+ VVE+DERVVL         A  A  D D                             GVTGE V V +  +    R  LQ++ D G  SLAVV LHSYTF  HE  +G+LA E+GFK +SLSS+VMPMVK+VPRG TT  DAYLTPVI  Y+ SF+SGFD   ++ V+  FMQSDGGL P+++F G  AILSGPAGGVVGYA T+ G                 AVIGFDMGGTSTDVSR+ GAF+HVFET TAG+T+QSPQLDI+TVAAGGGSRLFFRNGLF VGP+SAGAHPGPVCYRK G LAVTDANV LGRV+P+YFP IFGP EDE LD    + AF ++  ++N  AAA +  A  G    S   KTAD+VAYGF+RVANEAMCRPIRNLTTM+G D +TH LA FGGAGPQHCCA+A+ALGM RI+VSRFSGVLSAYG+  AD+V EKQE  A +Y E   E L  +  ++  LE AAR ELV QGF    +  + +LNLRF GTD ALMT  +  V             GG           D   +F + Y+REFGF LEGR V+VDDVRVR  GR                +  P PVD  S ++EG+GRV+TP++K   LKAG  V GPAIL+Q+VSTVVVEPGC A V+  G+  I VG V                 KR+   LDP+YLSIFAHRFMG+AEQMGRTLQRTSISVNIKERLDFSCA+FD  GGLVANAPHLPVHLGAMQEAVRYQ++HW    L+ GDVL+SNHPQLAGGSHLPD+TVITPVF EGR       FFVASRGHHADVGG+APGSMPP+SK L+EEGAAIVAFKLV  G F EAG+  LL AP     P CSG+RNLQDNLSDLRAQVAANT+G  L+QGL +EYGL VI AYM HIQ+NAE AVR ML  FSL   L EVGTV AED+MDDGTPI L VT+DR   SA+FDF GTG E+ GNLNAPPAVTASAVIY LRCLLP   AD+PLNQGCLVPVEI IP  SLLNPSP AAVVGGNVLTSQRVTDVIF+ F A AASQGCMNNLTFGD +MGYYETIAGGAGAGP+W G SGVHTHMTNTRITDPEILERR+PVIL  F LR  SGG G   GG+GV R++ FR+PL VS+LSERRAF+P+GLAGG    RGLNLL F GG  S + R V++GGKNTV+
Sbjct:    4 SFSFAIDRGGTFTDVFCVLPGN-GFRVLKLLSEDPANYPDAPREGIRRILEQETGVPHPRDEPLDTSRIASIKMGTTVATNALLERKGERFALAITAGFRDLLHIGNQSRPSIFDLKVRKPEVLYERVVEIDERVVL--------AAEDAASDCD---------------------------FVGVTGERVCVEKALNEDIARQQLQSLYDSGYRSLAVVLLHSYTFHRHELILGRLAREIGFKQVSLSSQVMPMVKMVPRGLTTCIDAYLTPVIDAYLASFKSGFDARLEERVRVSFMQSDGGLAPMNNFSGHHAILSGPAGGVVGYAVTASG------RAGRESEEAAQAVIGFDMGGTSTDVSRYDGAFQHVFETTTAGITIQSPQLDISTVAAGGGSRLFFRNGLFVVGPESAGAHPGPVCYRKGGVLAVTDANVLLGRVVPRYFPHIFGPREDEPLDVTAVQAAFADLTGEVNAFAAAESTQAREGGGEVSRVPKTADEVAYGFLRVANEAMCRPIRNLTTMRGFDITTHALACFGGAGPQHCCAIAKALGMRRIIVSRFSGVLSAYGIHLADIVVEKQEPAAGKYDE---ESLSTLEARLDALEKAAREELVAQGFAEDAIRVERFLNLRFAGTDCALMTARSEEVRTGGRQSGGGEWDGGRLGSSGMSQGRDYRSAFESNYQREFGFKLEGRDVLVDDVRVRVTGRXXXXXXXXXXXXXXXXK--PRPVDYGSVFFEGIGRVETPLYKAEGLKAGFVVPGPAILMQEVSTVVVEPGCTAHVTSEGNFEILVGAVE---------------EKRITTTLDPIYLSIFAHRFMGVAEQMGRTLQRTSISVNIKERLDFSCALFDETGGLVANAPHLPVHLGAMQEAVRYQLKHWAREGLREGDVLVSNHPQLAGGSHLPDITVITPVFDEGR-----LVFFVASRGHHADVGGLAPGSMPPMSKRLDEEGAAIVAFKLVEGGVFQEAGIIALLEAPARV--PGCSGSRNLQDNLSDLRAQVAANTQGRMLMQGLISEYGLGVIQAYMQHIQDNAEQAVRAMLTRFSLSQELPEVGTVGAEDFMDDGTPICLEVTIDRARGSAVFDFAGTGPELYGNLNAPPAVTASAVIYCLRCLLPD--ADIPLNQGCLVPVEILIPPSSLLNPSPSAAVVGGNVLTSQRVTDVIFRAFGAVAASQGCMNNLTFGDASMGYYETIAGGAGAGPSWHGESGVHTHMTNTRITDPEILERRFPVILRQFVLRDDSGGKGVYAGGDGVVRALEFRRPLVVSILSERRAFRPYGLAGGGDGARGLNLLTFAGGAKS-SRRLVNLGGKNTVQ 1274          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A2P6V631_9CHLO (5-oxoprolinase isoform A n=4 Tax=Micractinium conductrix TaxID=554055 RepID=A0A2P6V631_9CHLO)

HSP 1 Score: 1503 bits (3891), Expect = 0.000e+0
Identity = 834/1368 (60.96%), Postives = 976/1368 (71.35%), Query Frame = 1

Query:   34 FRFSIDRGGTFTDVYAEVPGSKGF---RVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAGGEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEVGDV--AGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWGSN-------LKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGDPSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKPDSAAS 4101
            F FSIDRGGTFTDVYAE  G+ G    RVLKLLSEDP NYPDAPREG+RR+LE ETG PHPR  PLDTSR+  IRMGTTVATNALLER+GE  ALV+T+GFRDLL+IGNQSRPNIFDLEI+ P++LY+ VVE DE VVL  G            D   T A                          VTGE V V R PD+A LR  LQ VLD GI S+AVV  H+  F  HEQ VG+LA EMGFK +SLSS VMPMVK+VPRGFT AADAYLTP I RY+++F++GFD     V   FMQSDGGLT V+ F G +AILSGPAGG VGYA T+  G              +  +IGFDMGGTSTDVSR+AGA+EHVFE+ TAGVT+Q+PQLDINTVAAGGGSRLF R+G+F VGP+SAGAHPGPVCYRK G+LA+TDAN+ LGR+LP +FPKIFGP E+E+LDSEG R A E +A ++N  AAAA        + P  K+ D++A GF+RVANE MCRPIR LT MKG+D + HVL+ FGGAG QH CA+A ALGM  I + R+SG+LSA G+  A+VV E QE  AA  G    + LP++  ++  L+  A   L ++GF    + CQ +LNLR+ GTD  LMTPA       PE GG               +F A Y+RE+GF LEGRA+ VDD+RVRA GR     +P  G  P      P P   AS Y+E  GR  TP + L +LK GH V GPA+LI ++ST+V+EPGC A V+   DV IE+G    A  XXXXXXXXXX         E DP+ L+IF+HRFMGIAEQMGR LQRTSISVNIKERLDFSCA+FD+ G LV+NAPHLPVHLGAM EAV+YQ++H+          L  GDVL+SNHPQLAGGSHLPD+TVITPVF  G        FFVASRGHHAD+GGI+PGSMPP S TLEEEGA I++ KLV  G FDEAG++ LL APG  G+P+ SGTRNLQDNLSDL+AQVAANT+G+AL++ L  EY L V+ AYM HIQ NAEAAVR+MLV +S   GL EVGTV+A D MDDGTPIRLAVTVDRR  SA+FDF GTG +V GN NAPPAVT SA+IYA+RCL+     D+PLN GC+ P+ ++IP GSLL+PSP AAVVGGNVLTSQRVTDV+ K F A AASQGCMNN TFGD  MGYYETIAGGAGAGP W G SGVHTHMTNTRITDPEILERRYPV+L  F LR GSGG G   GG+GVAR + F +PLT S+LSERRA +P+GL GG P + GLNL   R G        VS+GGK TV+V+ GD + ILTPGGGG G P + A+
Sbjct:    6 FSFSIDRGGTFTDVYAEFLGADGAPRSRVLKLLSEDPSNYPDAPREGIRRVLEAETGAPHPRGAPLDTSRVAAIRMGTTVATNALLERKGERTALVVTQGFRDLLHIGNQSRPNIFDLEIKCPDVLYEEVVECDEEVVLPLG------------DAPSTRAGPDPVGDSQRHPLVGER------AAAVTGEAVVVRRPPDMAGLRHELQRVLDAGIVSVAVVLKHAAIFPRHEQLVGRLAREMGFKQVSLSSVVMPMVKMVPRGFTAAADAYLTPHIMRYIQTFQAGFDAGLARVPVYFMQSDGGLTSVADFSGHKAILSGPAGGYVGYATTTRWGGVEPS---------RLQMIGFDMGGTSTDVSRYAGAYEHVFESTTAGVTIQAPQLDINTVAAGGGSRLFCRSGVFQVGPESAGAHPGPVCYRKGGYLAITDANLVLGRILPDFFPKIFGPLENESLDSEGARAALEAVAAEVNAQAAAAG-------QPP--KSVDEIAMGFLRVANETMCRPIRALTQMKGYDVTQHVLSCFGGAGGQHACAIAAALGMRSIFIHRYSGILSAVGIGLAEVVQEAQEPSAATLG---ADALPDLERRLDALQELAVARLRKKGFSDEQICCQRFLNLRYDGTDVPLMTPA-------PEAGGF------------AAAFEAAYKREYGFKLEGRAIRVDDLRVRASGRHTEPQQP--GREPGDAGPLPTPAVTASAYFEAGGRQPTPAYLLTDLKPGHAVTGPAMLIDNISTIVLEPGCTAHVTAARDVRIELGSAPAAAGXXXXXXXXXXGAAAGPADTECDPIQLAIFSHRFMGIAEQMGRVLQRTSISVNIKERLDFSCALFDADGNLVSNAPHLPVHLGAMSEAVKYQIRHYAPGGAGAAHGLAEGDVLVSNHPQLAGGSHLPDITVITPVFEGGS-----VVFFVASRGHHADIGGISPGSMPPHSHTLEEEGAHIISHKLVQGGHFDEAGITDLLLAPGRGGNPAVSGTRNLQDNLSDLKAQVAANTKGIALVKELIGEYSLPVVQAYMRHIQANAEAAVREMLVAYSRDQGLPEVGTVTAGDQMDDGTPIRLAVTVDRRDGSAVFDFEGTGPQVFGNTNAPPAVTHSALIYAMRCLV---TRDIPLNHGCMAPLTVKIPPGSLLSPSPDAAVVGGNVLTSQRVTDVVLKAFNAVAASQGCMNNFTFGDPGMGYYETIAGGAGAGPGWHGRSGVHTHMTNTRITDPEILERRYPVVLKQFMLRPGSGGAGRYRGGDGVARELEFLRPLTASILSERRAVRPFGLLGGQPGDAGLNLWLRRDG------MVVSLGGKATVQVEGGDCIRILTPGGGGFGPPTADAA 1299          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A835ZBL8_9STRA (Hydantoinase B/oxoprolinase-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBL8_9STRA)

HSP 1 Score: 1496 bits (3872), Expect = 0.000e+0
Identity = 836/1362 (61.38%), Postives = 966/1362 (70.93%), Query Frame = 1

Query:   13 MEKTERTFRFSIDRGGTFTDVYAEVPGSKGFRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKPA-VIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAGGEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEVGDVAGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWGSN-LKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGDPSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKPDS 4092
            M + +R F FSIDRGGTFTDVYA VPG K FR+LKLLSEDP NYPDAPREG+RRILE                              A++        L   +GFRDLL IGNQSR +IFDL I RPELLY+ VVEVDERVVL K                                             GVTGE VRV+R+PD+AALR  L AVL +G++SLAVVFLHSYT+A HE+ VG LA +MGF H+SLSSEVMPMVK VPRG+TTAADAYLTP+I +Y+ SF        DD++TLFMQSDGGLTP+S FRGSRAILSGPAGGVVGYAAT++  DA+          G+P  VIG DMGGTS+DVSR+AG +EHVFETVTAG+TLQSPQLDINTVAAGGGSRLF+R GLF+VGP+SAGAHPGP+CYRK G+LAVTDANV+LGR++PKYFP IFGP ED+ALD+EGP  AF  +A ++   +A+AAA         A    ++VA+GF+RVA EAMCRPIRNLTTMKG+D +TH LA FGGAGPQ CCA+A++LG+S I V R SGVLSAYGLS ADVV E+QE   A            + E+++ L+  A  +L++QGF    +    YLNLRF GTDT+LM+ A+ P                   D  L++F AQY+REFGF+LEGR V+VDD RVRAVG   PA RP+                R++    G+  +  P   L  +            +QDVSTVV+EP C A ++P GD+ + VG             XXXX   +     DP+YLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCA+FD+ GGLVANAPHLPVHLGAMQEAV+YQV+HWG++ L+ GDVL+SNHPQLAGGSHLPD+TVITPVF++   + A P+FFVASRGHHADVGGIAPGSMPPLSK+L EEGAAIVAFKLV  G F+E G+S LL            GTRNL DNLSDLRAQVAANTRG  L+Q L  EYGLDVI AYMGHIQ+NAE AVR MLV FSL  GL EVGTV+AED++DDGT I LAVT+DR+T SA FDF GTG EV GNLNAPPAVTASAVIY+LRCL+P    D+PLNQGCL PV I IP GSLL+P   AAVVGGNVLTSQRVTDVI K FRACAASQGCMNNLT GD  MGYYETIAGGAGAGP W GCSGVHTHMTNTRITDPEILERRYP++L  F LR  SGG G   GG+GV R + FRK L VS+LSERRAFQP+GL+GG P  RG NLLR+R G      R +S+GGKNT+ V  GDRLTILTPGGGG+G PDS
Sbjct:    1 MTQAKRDFGFSIDRGGTFTDVYATVPGPKKFRLLKLLSEDPDNYPDAPREGIRRILE------------------------------AIV------LPLPSMQGFRDLLLIGNQSRRHIFDLVIARPELLYEEVVEVDERVVLIK-------------------------------------------TVGVTGERVRVLRRPDVAALREQLSAVLARGVSSLAVVFLHSYTYAAHERAVGDLARDMGFAHVSLSSEVMPMVKAVPRGYTTAADAYLTPIITQYLASFXXXXXXXXDDIQTLFMQSDGGLTPMSQFRGSRAILSGPAGGVVGYAATAF--DASGRRH------GQPQPVIGLDMGGTSSDVSRYAGTYEHVFETVTAGITLQSPQLDINTVAAGGGSRLFYRGGLFSVGPESAGAHPGPICYRKGGYLAVTDANVFLGRIIPKYFPHIFGPREDQALDAEGPGAAFAALAAEVQGTSASAAA---------AAMAPEEVAHGFLRVAIEAMCRPIRNLTTMKGYDITTHALACFGGAGPQFCCAIAQSLGVSTIKVHRMSGVLSAYGLSLADVVAEQQEGTYAA-----------LQERLKRLDDRATNDLLKQGFDLEHIQHHHYLNLRFSGTDTSLMSEASAP-------------------DGFLRAFLAQYQREFGFLLEGRDVLVDDARVRAVGTRAPAERPK----------------RSTPLSPGIRAI--PRASLTRMVPQ---------LQDVSTVVIEPACEAAITPEGDITVAVGQ-----------GXXXXAGLKAGGPPDPIYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCALFDAAGGLVANAPHLPVHLGAMQEAVKYQVKHWGADGLRFGDVLVSNHPQLAGGSHLPDITVITPVFVD---NGAKPSFFVASRGHHADVGGIAPGSMPPLSKSLAEEGAAIVAFKLVEGGVFNEQGISKLLE-----------GTRNLADNLSDLRAQVAANTRGTKLMQDLTDEYGLDVIQAYMGHIQDNAERAVRDMLVAFSLSQGLPEVGTVTAEDFLDDGTRIALAVTIDRKTCSATFDFTGTGPEVYGNLNAPPAVTASAVIYSLRCLVPD--VDIPLNQGCLTPVNIIIPQGSLLSPCAEAAVVGGNVLTSQRVTDVILKAFRACAASQGCMNNLTLGDATMGYYETIAGGAGAGPGWHGCSGVHTHMTNTRITDPEILERRYPLLLLKFALRPDSGGTGQYKGGDGVERELMFRKDLVVSILSERRAFQPYGLSGGGPGARGKNLLRYRTG------RVISLGGKNTISVKEGDRLTILTPGGGGYGSPDS 1176          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A7S3H7L0_9STRA (Hypothetical protein n=2 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3H7L0_9STRA)

HSP 1 Score: 1493 bits (3866), Expect = 0.000e+0
Identity = 829/1419 (58.42%), Postives = 988/1419 (69.63%), Query Frame = 1

Query:   28 RTFRFSIDRGGTFTDVYAEV-PGSKGF----RVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKP-AVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAG--GEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCP---PPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEV--GDVAGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWGSNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGDPSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKPDSAASSMDVDDGVVGGAGGVVGDVEDPDRKSRREAEM--RLGGSVSGHRLGQE 4239
            + FRF+IDRGGTFTD+Y+EV   S G      V+KLLSEDP NY DAP EG+RRILE ETG+PHPR  P+DTSRIE+IRMGTTVATNALLER+GE  AL+ T GFR L  IGNQSRP IFDL+I+RP+LLY+ VVEVDERV+L K                                          +  G + E + V +  D+  +   L+ +L  GI SLAVVFLHSYTF +HE  V  +A ++GF+ +S+SSE+MPM++ VPRG TT  DAYLTPVI RY+KSF  GFD     V   FMQSDGGLTP+++F G++AILSGPAGGVVGYA TS    AA            P +VIGFDMGGTSTDVSR+AG +EHVFE+ TAGVT+QSPQLDINTVAAGGGSRLF++NGLF VGP+SAGAHPGPVCYRK G L VTDANV+LGRV P  FPKIFGP E+EALD EG R+AFE +  +IN+ A     H         V T  +VAYGFIR+ANEAM RPIRNLTTMKG+D + H LA FGGAGPQHCCA+A++LGM+++LV R+SG+LSAYGLS ADVV EKQE        +G   E L     +++ LE  AR +L+ QGF    +    YLN R+ GTDT+ MT     +                +D    + F   YRREFGF L  R ++VDD+RVRAVG++         AA L   S P   P  +  + + E L    TPV  + +L +G  V GPAI++Q+V+TV+VEPGC A ++P GD+AI V  GD                   R+  E+D +YLS+F+HRFMGIAEQMGRTLQRTS+SVNIKERLDFSCA+FD +GGLVANAPHLPVHLGAM EAVRYQV++WG +LK GDVL+SNHPQLAGGSHLPD+TVITP+F  G+       FFVASRGHHADVGGIAPGSMPPLSKTL +EGAAI+AFKLV  G F EAGV+ LL+APG+       GTRNLQDN+SDL+AQVAANT+G  L+  L  EY L+V+ AYMGHIQ  AE AVR+ML  FS + G+ EV   SAED++DDGTPIRLAVT+DR T SA+FDF GTG E+ GNLNAPPAVTASA+IY LRCLLP    D+PLNQGCL P+EI+IP   +LNPS  AAVVGGNVLTSQRVTDV+ + F+ACAASQGCMNNLTFGD  MGYYETIAGGAGAGPTW GCS VHTHMTNTRITDPEILE+RYP+IL  F LR GSGG G   GGEGV R + F KPL VS+LSERRAF+P+GLAGG    RG+NLLRF     +   R +S+GGKNT+ V  GD L I +PGGGG+G  D  A        V G AG         +  S + A +  R+ GS++ + L QE
Sbjct:   14 KRFRFAIDRGGTFTDIYSEVLDASTGAVIKKPVIKLLSEDPSNYKDAPTEGIRRILEAETGIPHPRSSPVDTSRIEFIRMGTTVATNALLERKGERIALLTTSGFRHLQLIGNQSRPKIFDLQIKRPDLLYEEVVEVDERVILVK-------------------------------DHTKDEFKAEDIRVGKSHEPLVVEKAIDVEEVTAKLRGILALGIKSLAVVFLHSYTFPDHEIIVKDIATQLGFEQVSISSEIMPMIRAVPRGGTTCVDAYLTPVIKRYLKSFSQGFDAGMAKVNVSFMQSDGGLTPMANFLGNKAILSGPAGGVVGYAITSELDHAARQRAAGPDVTLPPLSVIGFDMGGTSTDVSRYAGTYEHVFESTTAGVTIQSPQLDINTVAAGGGSRLFYKNGLFVVGPESAGAHPGPVCYRKGGPLTVTDANVFLGRVQPHLFPKIFGPNENEALDVEGTRVAFEALTEEINKDA-----HLHGK-----VYTTHEVAYGFIRIANEAMARPIRNLTTMKGYDVTKHSLACFGGAGPQHCCAIAKSLGMTQVLVHRYSGILSAYGLSLADVVVEKQEPF-----NSGVIAESLELANSRLKVLEQEARDDLISQGFPAESIEVTRYLNCRYHGTDTSSMTSVDARIASG-------------LDGSYEQEFVDGYRREFGFELLNRQILVDDIRVRAVGKAKTLIDEHASAA-LSAASVPAAVPDEEVQTFFDESLHT--TPVFFMHKLVSGQVVTGPAIIVQNVATVIVEPGCRADITPSGDIAITVQRGD-----------------HLRLSTEVDAIYLSVFSHRFMGIAEQMGRTLQRTSVSVNIKERLDFSCALFDPRGGLVANAPHLPVHLGAMSEAVRYQVKYWGDDLKEGDVLVSNHPQLAGGSHLPDITVITPIFDNGK-----IVFFVASRGHHADVGGIAPGSMPPLSKTLVQEGAAIIAFKLVRDGVFQEAGVTELLQAPGKL--EGNFGTRNLQDNISDLKAQVAANTKGANLMHELVKEYSLEVVVAYMGHIQACAETAVREMLRSFSTREGMAEVDVASAEDFLDDGTPIRLAVTIDRTTGSAVFDFAGTGPEIYGNLNAPPAVTASAIIYCLRCLLPDI--DIPLNQGCLNPIEIKIPPQCILNPSAGAAVVGGNVLTSQRVTDVVLRAFKACAASQGCMNNLTFGDATMGYYETIAGGAGAGPTWHGCSAVHTHMTNTRITDPEILEKRYPIILRQFALREGSGGKGQFNGGEGVVRELQFTKPLVVSILSERRAFRPYGLAGGEDGHRGMNLLRF-----AQDQRVISLGGKNTISVSTGDCLLIHSPGGGGYGSADGVA--------VQGTAG---------EAHSAQSASVPPRVSGSLNQYSLNQE 1322          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A8J9WPJ0_9CHLO (5-oxoprolinase n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9WPJ0_9CHLO)

HSP 1 Score: 1491 bits (3860), Expect = 0.000e+0
Identity = 825/1428 (57.77%), Postives = 980/1428 (68.63%), Query Frame = 1

Query:   13 MEKTERTFRFSIDRGGTFTDVYAEV---PGSKGFRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSY--GGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAGGEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEVGDVAGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWG-------SNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGD--PSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKPDSAASSMDVDDGVVGGAGGVVGDVEDPDRKSRR---EAEMRLGGSVSGHRLGQEQA 4245
            M   +R   FSIDRGGTFTDV+AE+    G K  RVLKLLSEDP NY DAPREG+RR+LEE +G PHPRD PLDTSRI  IRMGTTVATNALLER+GE C LV TKGFRDLL+IGNQSRP IFDLEIR P++LY  VVEVDE+VVL  G                                             VTGE V + R+PDL A+R  LQAVLD GI SLA+VF H+  + +HE+ VG++A E+GFK +SLSSEVM MVK+VPRGFT  ADAYLTP I RY+++F+SGFD+   DV+  FMQSDGGL+PVSSF G +A+LSGPAGG VGYA T+   G +AA           K  +IGFDMGGTSTDVSRFAG +EHVFE+ TAGVT+Q+PQLDINTVAAGGGSRLFFR G+F VGP+SAGAHPGPVCYRK G+ A+TDAN+ LGR+LP++FP IFGP ED+ LDS+  R A EE+A +INE         G  AKS      D+V  GF+RVANE MCRPIR LT MKG+D + H LA FGGAG QH CA+A+ LGM  I V R++GVLSA G+  ADVV E+QE  A E        + E+ E++  L+  A   L+ QGF+   +  + +LNLR+ GTD A+MT                       D    ++F + Y+REFGFILEGR + VDDVRVRA G++ P P+P  G         P P    S Y+E  GR DTP + L +L+ GH ++GPA+LI D+STVV+EP C+A ++   D+ I+VG  A                K+   E DP+ L+IF+HRFMGIAEQMGR LQRTSISVNIKERLDFSCA+F   G LVANAPHLPVHLGAM EA+RYQ++HWG         L+ GDVL SNHPQLAGGSHLPD+TVITPVF EG        FFVASRGHHADVGG+ PGSMPP S +L EEGAAI++FKLV  G F   G++ +L APG+  D  P  SGTRNL DNLSDL+AQVAAN +G++L+  L  EYGL  + AYM HIQ NAE AVRQMLV FS +  L EVGTV+AED MDDGTPIRL+VTVDRR  SA+FDF GTG +V GN NAPPAVT SA+IY+LRC++     D+PLNQGCL P+ +RIP GS+L+PSP AAVVGGNVLTSQRVTDV+ K F A AASQGCMNNLTFGDE MGYYETIAGGAGAGP WAG SGVHTHMTNTRITDPEILERRYPV+L+ F LR GSGG G   GG+GV R + F +P+T  +LSERRA +P+G+ GG  A  GLNLL       S   RTV++GGKN V++  G+RL ILTPGGGG G  D                       E P  K +R    A  R+GGSV+ ++  QE A
Sbjct:    1 MGSVQRDLSFSIDRGGTFTDVFAEIGTESGKKRQRVLKLLSEDPANYKDAPREGIRRVLEEVSGRPHPRDAPLDTSRILSIRMGTTVATNALLERKGEACCLVTTKGFRDLLHIGNQSRPAIFDLEIRVPDVLYSQVVEVDEQVVLPLG------------------EVPDKRAGRNPKQDSEQYPPGGPCTTSVTGEVVCIRRRPDLEAVRRDLQAVLDGGIRSLAIVFKHAAIYPDHEEAVGRVARELGFKQVSLSSEVMQMVKMVPRGFTATADAYLTPHIMRYIEAFQSGFDEGLRDVQLSFMQSDGGLSPVSSFCGHKAVLSGPAGGYVGYALTTQWEGQEAA-----------KLQMIGFDMGGTSTDVSRFAGTYEHVFESTTAGVTIQAPQLDINTVAAGGGSRLFFRTGVFQVGPESAGAHPGPVCYRKGGYAAITDANLVLGRILPEFFPNIFGPNEDQPLDSDAARQALEELARQINE------GSPGQPAKS-----VDEVTMGFVRVANETMCRPIRALTQMKGYDVAQHCLACFGGAGGQHACAIAQNLGMRTIFVQRYAGVLSAVGIGLADVVAEEQEPSAQELNST---TIRELDEQLDALQKKAVQRLLDQGFRDDQIAVERFLNLRYDGTDVAVMTSCP-------------------SDSTYTEAFESAYKREFGFILEGRGIRVDDVRVRATGKAVPLPKP--GDITSDPGPLPAPARVISAYFEVGGRQDTPAYLLKDLRPGHDIKGPAVLIDDISTVVIEPLCSAHITAGQDIRIQVGMEA-------------DGSKKPLTECDPIQLAIFSHRFMGIAEQMGRVLQRTSISVNIKERLDFSCALFGPDGSLVANAPHLPVHLGAMSEAIRYQIKHWGPEGEGAGEGLQPGDVLASNHPQLAGGSHLPDITVITPVFNEGE-----IRFFVASRGHHADVGGVTPGSMPPNSHSLAEEGAAILSFKLVRSGIFQGEGITDILMAPGKLSDSIPGISGTRNLSDNLSDLKAQVAANNKGISLVGDLIEEYGLPTVIAYMHHIQANAEDAVRQMLVAFSEEQQLAEVGTVTAEDQMDDGTPIRLSVTVDRRDGSAVFDFEGTGPQVFGNTNAPPAVTYSAIIYSLRCMV---HQDIPLNQGCLKPITVRIPPGSILSPSPTAAVVGGNVLTSQRVTDVVLKAFNAAAASQGCMNNLTFGDEGMGYYETIAGGAGAGPGWAGRSGVHTHMTNTRITDPEILERRYPVVLHCFSLRPGSGGPGHWRGGDGVVREIEFLRPITACILSERRAVRPFGILGGGAALAGLNLLL------SADGRTVNLGGKNNVQLRGGERLRILTPGGGGCGSADDKG--------------------EQPTVKKQRIDYSAIARVGGSVAKYKADQESA 1317          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A836BUI7_9CHLO (Uncharacterized protein n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A836BUI7_9CHLO)

HSP 1 Score: 1487 bits (3850), Expect = 0.000e+0
Identity = 857/1438 (59.60%), Postives = 997/1438 (69.33%), Query Frame = 1

Query:   28 RTFRFSIDRGGTFTDVYAEVPGSKG---FRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAG-GEGLPE-VWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAP----RPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEV----------GDVAGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWG-------SNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFME-----GRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGD--PSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKPDS-AASSMDVDDGVVGGAGGVVGDVEDPDRKSRREAEMRLGGSVSGHRLGQE 4239
            R F FSIDRGGTFTDV+AEVP  KG   FRVLKLLS DP NYPDAPREG+RR+LEE TGVPHPR  PLDTSRI  IRMGTTVATNALLER+GE  AL ++ GF DLL+I NQSRPNIFDLEIR P+ LY+ VVEVDE+VVL                    T                       +   VTGE V V R+ D   LR  LQ VLD GITSLAVV  H+  F EHE+ VG LA  MGF  +SLS EVMPMVK+VPRGFT AADAYLTP I RY+  F +GFD+   DV+ LFMQSDGGL+PV +F G +AILSGPAGG VGYA T+                 K  VIGFDMGGTSTDVSR+AG++EHVFET TAGVT+Q+PQLDINTVAAGGGSRL FRNGLFAVGP+S GAHPGPVCY+K G LA+TDAN+ LGR+LP++FPKIFGPGE+E LD+EG   AF  +  ++N   A   A        P   T DDVA GF+RVANEAMCRPIR LT M+G+DA+ HVL  FGGAG QH CA+A+ALGMS I V R++G+LSA G+  ADVV E QE  AA  G AG G G  E +  +   LEAAA  +L+ QGF    +  + +LNLR+ GTD A+MT         P PGG               +F A YRREFGF+L+ R V VDDVRVRAVGRS P P     P     PL     P PV   S Y+E  GR  TP + L  L  GH +EGPA+LI  +ST+VVEPG  ATV+   +V ++           G  A    XXXXXXXXXXX     R+ DPV L+IF+HRFMGIAEQMGRTLQRTS+SVNIKERLDFSCA+F   G LVANAPHLPVHLGAM EAVR+Q++++G         L+ GDVLLSNHPQLAGGSHLPD+TVITPVF +     G G      FFVASRGHHAD+GGI+PGSMPP SK L EEGAA+V+FKLV +G+F E G++ LL APG   D  P  SGTRNL DNLSDL+AQVAANTRG+ L+  L  EY L V++A+MGHIQ NAEA+VR+ML +FS++ GL EVGTV A D MDDGTPI LAVT+DRR  SA+FDF GTG EV GN NAPPAVT SA+IYALRC++    +D+PLNQGCL P+ +RIP   +LNPS  AAVVGGNVLTSQRVTDV+ K F A AASQGCMNN TFGDE MGYYETIAGGAGAGP W G SGVHTHMTNTRITDPEILERRYPV+L++F LR GSGG G   GG+GV R +   +P++  +LSERRA +P+GL GG  A+ GLNLL  +GG      RTV++G K TV++DAGDRL ILTPGGGG+G P +  A++  +    +GG  G  G  ED     RR A   L GSV  +R  QE
Sbjct:    2 RDFHFSIDRGGTFTDVFAEVPDGKGGTKFRVLKLLSVDPSNYPDAPREGIRRVLEEVTGVPHPRSQPLDTSRIASIRMGTTVATNALLERKGERAALAVSSGFPDLLHIANQSRPNIFDLEIRTPDKLYEQVVEVDEQVVLP------------------LTDAPSQRNGAQAAHNSSRFPPSGFLEAAVTGEVVEVRRRLDPEGLRRDLQKVLDSGITSLAVVLKHAAIFPEHEREVGALARSMGFTQVSLSHEVMPMVKMVPRGFTAAADAYLTPHIRRYLADFTAGFDEGLKDVQVLFMQSDGGLSPVDAFSGHKAILSGPAGGYVGYALTTRWAGLDLD---------KLQVIGFDMGGTSTDVSRYAGSYEHVFETTTAGVTIQAPQLDINTVAAGGGSRLVFRNGLFAVGPESVGAHPGPVCYKKGGALAITDANLQLGRILPEFFPKIFGPGENEPLDAEGTAAAFRAVTAEVNAHEATTGA--------PTTSTTDDVAMGFVRVANEAMCRPIRALTNMRGYDAAAHVLGCFGGAGGQHACAIAKALGMSTIFVHRYAGILSAVGIHLADVVSEAQEPAAARLGPAGPGLGPDEDLLARQARLEAAAVQKLLDQGFSRDQISTERFLNLRYHGTDVAVMT-------AEPGPGGGGPGGYGA-------AFEAAYRREFGFVLD-REVWVDDVRVRAVGRSRPLPDDAMAPVEAPGPL-----PAPVTTTSAYFEPRGRQPTPAYALDALAPGHALEGPALLIDAISTIVVEPGWRATVTGGHNVRMDALDGAHTAAGAGARAVAAAXXXXXXXXXXXXXXXARDADPVRLAIFSHRFMGIAEQMGRTLQRTSVSVNIKERLDFSCALFGPDGSLVANAPHLPVHLGAMSEAVRFQIRYYGPGGPGELEGLQEGDVLLSNHPQLAGGSHLPDITVITPVFADAPAGAGAGGKREIVFFVASRGHHADIGGISPGSMPPTSKLLVEEGAAVVSFKLVRRGAFQEEGITQLLLAPGRLADRIPGISGTRNLADNLSDLKAQVAANTRGIQLVGELIREYSLGVVAAFMGHIQANAEASVREMLREFSVRQGLREVGTVRASDAMDDGTPICLAVTIDRRDGSAVFDFEGTGPEVYGNHNAPPAVTYSAIIYALRCMV---HSDIPLNQGCLAPITVRIPDACILNPSDTAAVVGGNVLTSQRVTDVVLKAFEAAAASQGCMNNFTFGDEQMGYYETIAGGAGAGPGWHGRSGVHTHMTNTRITDPEILERRYPVVLHSFRLRPGSGGEGCWRGGDGVVREIEALRPMSAGILSERRALRPFGLLGGGAAQPGLNLLLRKGG------RTVNLGAKATVQLDAGDRLRILTPGGGGYGPPGADEATAQGIKRRRLGG--GDEG-AEDGAAGGRRVA---LKGSVEEYRSRQE 1369          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A2P6TTJ8_CHLSO (5-oxoprolinase n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TTJ8_CHLSO)

HSP 1 Score: 1482 bits (3837), Expect = 0.000e+0
Identity = 830/1381 (60.10%), Postives = 973/1381 (70.46%), Query Frame = 1

Query:   28 RTFRFSIDRGGTFTDVYAEVPGSKG---FRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAGGEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEV-------------------GDVAGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWGSN------LKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGDPSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKP 4086
            R F FSIDRGGTFTDVYAE   ++G    RVLKLLSEDP NYPDAPREG+RR+LE E+GVPHPR  PLDT RI  IRMGTTVATNALLER+GE  ALV+T GFRDLL+IGNQSRP IFDLEIR P++LY+ VVE DE+VVL  G            D   T A                          VTGE V V + PDL ALRG LQ VLD GI S+AVV  H+  +  HEQ VG+LA +MGF  +SLSS VMPMVK+VPRGFT AADAYLTP I RY+++F++GFD+    V   FMQSDGGLT V  F G +AILSGPAGG VGYA T+  G A            K  +IGFDMGGTSTDVSR+AG +EHVFE+ TAGVT+Q+PQLDINTVAAGGGSRLFFR+G+F VGP+SAGAHPGPVCYRK G+LA+TDAN+ LGR+LP++FP IFGP EDE LD+EG R A E +A ++N  AAAA    G  +KS      D+VA GF+RVANE MCRPIR LT MKG+D + HVLA FGGAG QH CA+A ALGM  I V R+SG+LSA G+  A+VV E QE  AA       E LPE+  ++  L+  A  +LV +GF    V CQ +LNLR+ GTD  +MTPA                     + D   +F   Y+REFGF LE R ++VDD+RVRA GR   A  P  G AP      P P   +S Y+E  GR  TP + L++L+ GH V GPAILI ++ST+VVEP C A ++   DV +E+                        XXXXXXXXXXXXXX    + E DP+ L+IF+HRFMGIAEQMGR LQRTSISVNIKERLDFSCA+FDS G LV+NAPHLPVHLGAM EAV++Q++++ S       L+ GDVL+SNHPQLAGGSHLPD+TVITPV+   R       FFVASRGHHAD+GGI+PGSMPP S +L EEGAAI++ KLV  G FDE G++ LL APG  GDP+ SGTRNLQDNLSDL+AQVAANT+G+AL++ L  EYGL V+ AYM HIQ NAE AVR+MLV FS + GL EVGTV+  D MDDGTPI L+VT+DRR  SA FDF GTG EV GN NAPPAVT SA+IYALRC++     D+PLN GC+ P+ + IP G LL+PSP AAVVGGNVLTSQRVTDV+ K F A AASQGCMNN TFGDE MGYYETIAGGAGAGP W G SGVHTHMTNTRITDPEILERRYPV+L+ F LR G+GG G   GG+GV R + F +PLT S+LSERR+ +P+GL GG P   GLNL   R G      R VS+GGK TV+V+ GDRL ILTPGGGG+G P
Sbjct:    8 RDFSFSIDRGGTFTDVYAEFRDAQGQPRTRVLKLLSEDPGNYPDAPREGIRRVLEAESGVPHPRGQPLDTHRIAAIRMGTTVATNALLERKGERTALVVTAGFRDLLHIGNQSRPAIFDLEIRCPDVLYETVVECDEQVVLPLG------------DAPSTRAGRDPAGDSQRHPLQGSP------ATAVTGEAVVVRKAPDLEALRGELQRVLDSGICSVAVVLKHAAIYPAHEQLVGQLARDMGFTQVSLSSVVMPMVKMVPRGFTAAADAYLTPHILRYIETFQAGFDEGLKKVPVYFMQSDGGLTSVGDFSGHKAILSGPAGGYVGYAMTTRWGGA---------DASKLQLIGFDMGGTSTDVSRYAGRYEHVFESTTAGVTIQAPQLDINTVAAGGGSRLFFRSGVFQVGPESAGAHPGPVCYRKGGYLAITDANLVLGRILPQFFPHIFGPQEDEPLDAEGARAAMEAVAGEVNAHAAAA----GQPSKS-----VDEVAMGFVRVANETMCRPIRALTQMKGYDVTQHVLACFGGAGGQHSCAIAAALGMRTIFVHRYSGILSAVGIGLAEVVQEAQEPSAAVLS---SEALPELNRRLDALQEQALAKLVDKGFSAQQVSCQRFLNLRYDGTDVPIMTPAP-------------------ANGDFAAAFEQAYQREFGFTLEKRRILVDDLRVRASGRH--AELPEAGEAPQHAGPLPQPAATSSAYFEVGGRQPTPAYLLSDLQPGHAVPGPAILIDNISTIVVEPRCTAHITATRDVRLELPPPRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVAETECDPIQLAIFSHRFMGIAEQMGRVLQRTSISVNIKERLDFSCALFDSDGNLVSNAPHLPVHLGAMSEAVKFQIRYYASGAGAAEGLQEGDVLVSNHPQLAGGSHLPDITVITPVWEGSR-----VVFFVASRGHHADIGGISPGSMPPHSHSLREEGAAIISHKLVQGGHFDEEGITGLLLAPGRGGDPAISGTRNLQDNLSDLKAQVAANTKGIALVKELIGEYGLPVVQAYMKHIQANAELAVREMLVAFSQEQGLPEVGTVTTRDQMDDGTPICLSVTIDRRDGSATFDFEGTGPEVFGNTNAPPAVTHSAIIYALRCMV---TRDIPLNHGCMAPITVCIPKGCLLSPSPEAAVVGGNVLTSQRVTDVVLKAFNAAAASQGCMNNFTFGDEGMGYYETIAGGAGAGPGWHGRSGVHTHMTNTRITDPEILERRYPVVLHQFRLRPGTGGEGQYRGGDGVVRELEFLRPLTASILSERRSVRPFGLLGGQPGAAGLNLWLKRDG------RMVSLGGKATVQVEGGDRLRILTPGGGGYGPP 1314          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A8J4AQ69_9CHLO (Uncharacterized protein n=3 Tax=Volvox TaxID=3066 RepID=A0A8J4AQ69_9CHLO)

HSP 1 Score: 1457 bits (3772), Expect = 0.000e+0
Identity = 844/1444 (58.45%), Postives = 999/1444 (69.18%), Query Frame = 1

Query:   28 RTFRFSIDRGGTFTDVYAEVPGSKG---FRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATS--YGGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAG-GEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQES-CPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEVGDVAGXXXXXXXXXXXXXXR----KRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHWGSN-------LKAGDVLLSNHPQLAGGSHLPDMTVITPVFME-------------GRGDDAGP---AFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGD--PSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHGKPDSAASSMDVDDGVVG----GAGGVVGDVEDPDRKSRREAEMRLGGSVSGHRLGQE 4239
            R F FSIDRGGTFTDV+AEVP   G   F+VLKLLS DP NYPDAPREG+RR+LEE TGV HPRD PLDT RI  IRMGTTVATNALLER+GE  AL +T GF DLL+I NQSRPNIFDLEIR PE LY+ VVEV+E+VVL  G   G    A                                +   VTGE V V R+ D   LR  L+A+L +GI SLAVV  H+  +  HEQ+VG +A EMGF  +SLS EVMPMVK+VPRGFT AADAYLTP I RY+  F +GFD+ F DV+ LFMQSDGGL+PV+ F G +A+LSGPAGG VGYA T+   G D +           K  VIGFDMGGTSTDVSR+AG++EHVFET TAGVT+Q+PQLDINTVAAGGGSRL FRNGLFAVGP+S GAHPGPVCY+K G LA+TDAN+ LGR+LP++FPKIFGP E+E LD EG   AF ++ +++N+   +  A        P   + DDVA GFI VANEAMCRPIR LT M+G+DA+ H LA FGGAG QH CA+AR+LGMS I V R++G+LSA G+  ADVV E QE  A+  G AG GE   E+ E+   LE  A  +L+ QGF    +  + YLNLR+ GTD A+MT A P          XXXXX          +F + YRREFGF+L+ R + VDDVRVRAVGRS P P     A  +G+    PPP    S Y+E  GR  TPVH L  L+ GH + GPA+LI  +ST+VVEPG  A ++   +V ++    A XXXXXXXXXXX             E DPV L+IF+HRFMGIAEQMGRTLQRTS+SVNIKERLDFSCA+F   G LVANAPHLPVHLGAM EAVR+QV+++ S        L+  DVLLSNHPQLAGGSHLPD+TVITPVF               G G   G     FFVASRGHHAD+GGI+PGSMPP SK L EEGAA+V+FK+V +G F E G++ LL APG+  D  P  SGTRNL DNLSDL+AQVAANTRG+ L+  L +EYGL+V+SA+M HIQ NAE +VR ML DFS++ GL E GTV A D MDDG+PI L+VT+DRR  SA+FDF GTG EV  N NAPPAVT SA+IY+LRC++    +D+PLNQGCL P+ +RIP GS+LNPS  AAVVGGNVLTSQRVTDV+ K F A AASQGCMNN TFGDE MGYYETIAGGAGAGP W G SGVHTHMTNTRITDPEILERRYPV+L+ F LR GSGG G   GG+GV R +   +P++  +LSERRA +P+GL GG  AE G+NLL    G      R V++G K TV+++AGDRL ILTPGGGG+G P     + DV  GV+     G G  +G  ++ +   RR A   L GSV  +R  QE
Sbjct:    2 REFHFSIDRGGTFTDVFAEVPDGNGGSKFKVLKLLSVDPANYPDAPREGIRRVLEEVTGVAHPRDSPLDTCRIASIRMGTTVATNALLERKGERAALAVTAGFPDLLHIANQSRPNIFDLEIRTPEKLYEEVVEVEEQVVLPLGASPGQRNGAQA------------------ALNSTRYPPVGQLEAAVTGEVVEVRRRLDTEKLRAGLKAILAKGIRSLAVVLKHAAIYPAHEQQVGAIAREMGFTQVSLSHEVMPMVKMVPRGFTAAADAYLTPHIRRYIADFTAGFDEGFKDVQVLFMQSDGGLSPVNQFSGHKAVLSGPAGGYVGYALTTRWVGMDLS-----------KRQVIGFDMGGTSTDVSRYAGSYEHVFETTTAGVTIQAPQLDINTVAAGGGSRLMFRNGLFAVGPESVGAHPGPVCYKKGGMLAITDANLQLGRILPQFFPKIFGPLENEPLDEEGTAAAFRQLTSEVNKFEESTGA--------PNCSSVDDVAMGFISVANEAMCRPIRALTQMRGYDAAAHTLACFGGAGGQHACAIARSLGMSNIFVHRYAGILSAVGIHLADVVAEVQEPAASRLGPAGPGE---EIMERQARLEEQAVQKLLDQGFTRDQITAERYLNLRYHGTDVAVMT-AEPXXXXXXXXXXXXXXXPAAY----ATAFASSYRREFGFVLD-RDIWVDDVRVRAVGRSRPLPDD--AAVEVGEPGPLPPPAITTSAYFEPGGRQPTPVHHLESLRPGHALLGPALLIDTISTIVVEPGWRAIITGGRNVQMKAVGPAXXXXXXXXXXXXGASAFTSTAPTAVECDPVRLAIFSHRFMGIAEQMGRTLQRTSVSVNIKERLDFSCALFGPDGSLVANAPHLPVHLGAMSEAVRFQVRYYSSGGPGAAEGLEERDVLLSNHPQLAGGSHLPDITVITPVFSADGEDGXXXXXXXIGXGRGGGRRRIVFFVASRGHHADIGGISPGSMPPTSKVLAEEGAAVVSFKIVRKGIFQEEGITELLLAPGKLADRIPGISGTRNLSDNLSDLKAQVAANTRGIQLVGDLISEYGLEVVSAFMDHIQRNAELSVRAMLRDFSVRQGLAETGTVYARDIMDDGSPIVLSVTIDRREGSAVFDFEGTGPEVFANHNAPPAVTYSAIIYSLRCMV---QSDIPLNQGCLAPITVRIPPGSILNPSDTAAVVGGNVLTSQRVTDVVLKAFEAAAASQGCMNNFTFGDEQMGYYETIAGGAGAGPGWHGRSGVHTHMTNTRITDPEILERRYPVVLHAFKLRPGSGGEGCWRGGDGVVREVEALRPMSAGILSERRAARPFGLLGGKDAEPGINLLLRSNG------RIVNLGAKATVQLEAGDRLRILTPGGGGYGPP----GADDVTAGVLKRRRVGCGDRLGGGDEVE--GRRVA---LKGSVEEYRNRQE 1379          

BLAST of mRNA_P-fluviatile_contig14.2076.1 vs. uniprot
Match: A0A061QPQ7_9CHLO (5-oxoprolinase (ATP-hydrolysing) n=1 Tax=Tetraselmis sp. GSL018 TaxID=582737 RepID=A0A061QPQ7_9CHLO)

HSP 1 Score: 1453 bits (3762), Expect = 0.000e+0
Identity = 799/1365 (58.53%), Postives = 945/1365 (69.23%), Query Frame = 1

Query:   13 MEKTERTFRFSIDRGGTFTDVYAEVPGSKG---FRVLKLLSEDPHNYPDAPREGVRRILEEETGVPHPRDGPLDTSRIEYIRMGTTVATNALLEREGEPCALVITKGFRDLLYIGNQSRPNIFDLEIRRPELLYKAVVEVDERVVLDKGGEGGWEAAAAVDDGDGTTAXXXXXXXXXXXXXXXXXXXXXXVVQGVTGEGVRVVRKPDLAALRGSLQAVLDQGITSLAVVFLHSYTFAEHEQRVGKLAEEMGFKHLSLSSEVMPMVKVVPRGFTTAADAYLTPVIARYVKSFRSGFDQNFDDVKTLFMQSDGGLTPVSSFRGSRAILSGPAGGVVGYAATSYGGDAAXXXXXXXXXGGKPAVIGFDMGGTSTDVSRFAGAFEHVFETVTAGVTLQSPQLDINTVAAGGGSRLFFRNGLFAVGPQSAGAHPGPVCYRKSGHLAVTDANVYLGRVLPKYFPKIFGPGEDEALDSEGPRLAFEEMANKINELAAAAAAHAGPDAKSPAVKTADDVAYGFIRVANEAMCRPIRNLTTMKGHDASTHVLATFGGAGPQHCCAMARALGMSRILVSRFSGVLSAYGLSAADVVCEKQEACAAEYGEAGGEGLPEVWEKVRGLEAAARLELVQQGFKPGDVYCQAYLNLRFQGTDTALMTPATPPVHKAPEPGGXXXXXXXXMDDDALKSFFAQYRREFGFILEGRAVVVDDVRVRAVGRSPPAPRPRVGAAPLGQESCPPPVDRASCYWEGLGRVDTPVHKLAELKAGHRVEGPAILIQDVSTVVVEPGCAATVSPFGDVAIEVGDVAGXXXXXXXXXXXXXXRKRVKRELDPVYLSIFAHRFMGIAEQMGRTLQRTSISVNIKERLDFSCAVFDSKGGLVANAPHLPVHLGAMQEAVRYQVQHW------GSNLKAGDVLLSNHPQLAGGSHLPDMTVITPVFMEGRGDDAGPAFFVASRGHHADVGGIAPGSMPPLSKTLEEEGAAIVAFKLVTQGSFDEAGVSTLLRAPGERGDPSCSGTRNLQDNLSDLRAQVAANTRGVALLQGLAAEYGLDVISAYMGHIQENAEAAVRQMLVDFSLQMGLGEVGTVSAEDYMDDGTPIRLAVTVDRRTRSALFDFGGTGKEVLGNLNAPPAVTASAVIYALRCLLPGDAADVPLNQGCLVPVEIRIPAGSLLNPSPRAAVVGGNVLTSQRVTDVIFKCFRACAASQGCMNNLTFGDEAMGYYETIAGGAGAGPTWAGCSGVHTHMTNTRITDPEILERRYPVILNTFGLRRGSGGGGARPGGEGVARSMTFRKPLTVSVLSERRAFQPWGLAGGLPAERGLNLLRFRGGGGSGASRTVSIGGKNTVKVDAGDRLTILTPGGGGHG 4080
            +E   + F FSIDRGGTFTDV+AEVP + G   +R LKLLSEDP NY DAPREG+RRILEE TG  HPR+ PLDTSRI  IRMGTTVATNALLER+GE   LV TKGF DLL+IGNQSRP+IFDLE+R PE LY  VVEVDE VVL  G + G  A    D G    A                       V  VTGE V V R PD+ A+R  L+A+L  GIT++ VV  H+  F EHE++VG+LA  MGF+ +SLSSEVMPMVK+VPRG+T AADAYLTP I RY+ +F +GFD+    V+  FMQSDGGL+PV  F G +AILSGPAGGVVGYA T+                G   VIGFDMGGTSTDVSR+AG FEHVFET TAGVT+Q+PQLDINTVAAGGGSRLFFRNG+F+VGP+SAGAHPGPVCYRK GHLAVTDAN+ LGRV P  FPKIFGP ED+ LD +G R AFEE+A  IN         A  +      K+ D+VAYGF+ VANEAMCRPIR +T MKG+D S HVLA FGGAG QH CA+AR+LGMS + V RFSG+LSA G+  +DVV E Q    +  G   G   PE+  ++  LEA AR  L  QGF    +  + +LNLR+ GTD A+M        + PE            D +   +F  +YRREFGF+L  R V+ DDVRVRA GRS         AAP      P P      Y+   GR  TPV ++ EL+ GH V GPAIL+   STVVVEP CAA ++  GD+ I++ D A                K+V  E DP+ L+IF+HRFM IAEQMGR LQRTSISVNIKERLDFSCA+F   G LV+NAPH+PVHLG+M ++V+    ++         L  GDVLLSNHPQLAGGSHLPD+TV+TPVF  G+       F+VASR HHAD+GGIAPGSMPP SK LEEEGAAIVAFKLV++G+F E G+  LL APG+ G P  SGTRNL+DNLSDL+AQVAAN +G+ L++ L  EY +  + AYM HIQ NAE AVRQML DFS ++GL E+G+V AED MDDG+PI L VT+DR+ +SA FDF GTG EV GN N PPAV  SA+IY LRC++     D+PLNQGCL P+E+ IP  S+LNPS  AAVVGGNVLTSQRVTDV+ K F A AASQGCMNNLTFGD  +GYYETIAGGAGAGPTW G SGVHTHMTNTRITDPEILERRYPV+L  F LR+GSGG G   GG+GV R + F +PL   +LSERR+ +P+GL GGLP + G+NLL+ + G      R VS+G K +  V+AGD L I TPG GG+G
Sbjct:   74 LEMHGKDFSFSIDRGGTFTDVFAEVPRTGGGTEYRTLKLLSEDPENYADAPREGIRRILEEVTGEVHPRERPLDTSRIRSIRMGTTVATNALLERKGERFGLVTTKGFADLLHIGNQSRPDIFDLEVRCPENLYDRVVEVDESVVLPLGDKPGRRAGP--DPGRSAAAGGPAGR----------------TVTSVTGEVVTVRRPPDMGAVRRDLEALLGDGITAICVVLKHAAIFPEHEKQVGELARSMGFRQVSLSSEVMPMVKMVPRGYTAAADAYLTPRIMRYLSTFTAGFDEGLASVQLAFMQSDGGLSPVDGFSGHKAILSGPAGGVVGYALTTR---------WEGTEPGSLQVIGFDMGGTSTDVSRYAGKFEHVFETTTAGVTIQAPQLDINTVAAGGGSRLFFRNGVFSVGPESAGAHPGPVCYRKGGHLAVTDANLLLGRVQPDLFPKIFGPTEDQPLDLDGTRAAFEELAAAIN---------ADAERMGLPPKSLDEVAYGFLEVANEAMCRPIRAMTQMKGYDCSAHVLACFGGAGGQHACAIARSLGMSTVFVHRFSGILSAVGIGMSDVVVEAQGPAQSRVG--AGALPPELESRLNELEAEARAGLAAQGFGDHQIETERFLNLRYDGTDIAVMI-------RTPE------------DGNFRAAFDEEYRREFGFVLSDRDVLADDVRVRATGRSGMFLGEE--AAPADPGELPLPAMDVPVYFADGGRQPTPVFRIGELRPGHSVAGPAILVDKNSTVVVEPSCAAHITSRGDIRIQIEDPAD---------------KKVSTECDPIQLAIFSHRFMSIAEQMGRALQRTSISVNIKERLDFSCALFGPDGSLVSNAPHMPVHLGSMSQSVKAVDAYYTAGPGAAEGLHEGDVLLSNHPQLAGGSHLPDLTVVTPVFNNGK-----KVFYVASRAHHADIGGIAPGSMPPNSKLLEEEGAAIVAFKLVSKGTFQEEGLKELLMAPGKSGIPGNSGTRNLEDNLSDLKAQVAANNKGIQLVRDLIDEYSMATVQAYMSHIQVNAEEAVRQMLTDFSREIGLPEIGSVLAEDQMDDGSPIVLKVTIDRKNQSATFDFTGTGPEVYGNCNTPPAVVYSAIIYCLRCMV---TRDIPLNQGCLTPIEVIIPENSILNPSAGAAVVGGNVLTSQRVTDVVLKAFSAAAASQGCMNNLTFGDSGLGYYETIAGGAGAGPTWEGRSGVHTHMTNTRITDPEILERRYPVVLRCFELRKGSGGLGRFKGGDGVVRELEFLRPLQAGILSERRSSRPFGLNGGLPGKSGVNLLKKKDG------RVVSLGPKASFPVEAGDVLRIETPGAGGYG 1350