mRNA_P-fluviatile_contig13.1737.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: D7G858_ECTSI (Leucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G858_ECTSI)

HSP 1 Score: 1560 bits (4040), Expect = 0.000e+0
Identity = 771/857 (89.96%), Postives = 808/857 (94.28%), Query Frame = 1

Query:  601 GTKLYDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTEETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMANAEGDPRKGDDHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDVELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTFEKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 3171
            G+KLY+HK IEARWQ+HWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTD +YVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERG HPVVR PLRQWAL+ITKYADQLEAGLEGL WP+GTL AQKQWIGRSEGASITFDLEGT E++EVFTTRPDTLMGVTY+VLAPEHPLTL+VAT E+RAAVEEYV ASASRSDLDR+SMGKGKKKTGTDTGA+AVHP++GEKVPVWTADYVLGSYGTGAVMAVPAHDERD AFA+ F LP++RVV P+GGK XXXXXXXXXXXXXXX     E            V+EAFTG GVA+N GEG DGLSTE+CAEAVV QLE +QHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM + EGDPR+GDDHKICYD PMPVDVSELPLTLPEMESFEPGDDPQGCLTR PDWR+FQRDGKWFARETNTMPQWAGSCWYYLRFTD KN +AGWSK+ADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILG DGEKMSKSRGNVINPDDIVD+ GADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDVEL  ETLT+LHKTMKKVSEDI+ELAFNTAISALMVLTNHLMTFEKPPREALEALV MVSPFAPHLGEECWDLLGNN G+G++FAPWVEWKEELCVS++VTLGVQVNGKVR ELELSKTASS+DARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK
Sbjct:   15 GSKLYNHKAIEARWQQHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDKDYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGDHPVVRTPLRQWALKITKYADQLEAGLEGLNWPDGTLTAQKQWIGRSEGASITFDLEGTGESMEVFTTRPDTLMGVTYLVLAPEHPLTLEVATQERRAAVEEYVTASASRSDLDRTSMGKGKKKTGTDTGALAVHPITGEKVPVWTADYVLGSYGTGAVMAVPAHDERDLAFARTFELPVRRVVKPSGGKXXXXXXXXXXXXXXXXXXXXXE------------VVEAFTGRGVAINSGEGFDGLSTEECAEAVVSQLESQQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMVDPEGDPRRGDDHKICYDQPMPVDVSELPLTLPEMESFEPGDDPQGCLTRAPDWRFFQRDGKWFARETNTMPQWAGSCWYYLRFTDTKNADAGWSKKADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGTDGEKMSKSRGNVINPDDIVDSDGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDVELAPETLTILHKTMKKVSEDIDELAFNTAISALMVLTNHLMTFEKPPREALEALVRMVSPFAPHLGEECWDLLGNNKGNGIAFAPWVEWKEELCVSDSVTLGVQVNGKVRTELELSKTASSDDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 859          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A7S4DRP5_9EUKA (Leucine--tRNA ligase n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S4DRP5_9EUKA)

HSP 1 Score: 1126 bits (2913), Expect = 0.000e+0
Identity = 562/859 (65.42%), Postives = 670/859 (78.00%), Query Frame = 1

Query:  607 KLYDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTEETVEVFTTRPDTLMGVTYIVLAPEHPL--TLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMANAEGDPRKGDDHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDVELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTFEKP---PREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVV 3168
            K YD  +IE RWQK W E+ TFKTPER +G+ K+++LDMFPYPSG+GLHVGHPEGYTASDVMARY+RMK +DVLHPMGWDSFGLPAEQHAI TGTHP VTT  NI  F+RQL MLGFSYDWDRE+ATTD  +VKWTQWIFL+L+K GLA QSE  VNWCPALGTVLANEE+I+GKSERG HPV R+PLRQW L+IT+YAD+L   L+GL WPEGTL AQ+ WIGRSEGA I F+++G +E++ VFTTRPDTLMGVTY+ +APEHPL  TL +  D     V  YV  ++S+SD+DR++    K+KTG DTGA A+HP++GE++P+W ADYVLGSYG+GAVMAVPAHDERDFAFA  F LP K VV     K   XXXXXXXXXX    +  E+E       KA+P   AFTG G  VN GE  DGL  ++    ++++LE    GR++I Y+LRDWIFSRQRYWGEPIPIYFPV MA+  GDPRKGD+H I Y  P+PV   ELPL LPEM++F+PGDDPQGCL R  DWRYF +DGKW+ARETNTMPQWAGSCWYYLRF D  N +  +SKQAD DWMPVDLYVGGQEHA LHLLYARFWHKVL++ G  K  EPFQ+LVHQGMILG DGEKMSKSRGNV+NPDD+V+ +GAD+LRLYEMFMGPLEATKPWQT QV+GV RFRD+VY LA RG     ++ +    +HK +KKV+ED+E ++FNTAISA+MV TN L    K    PR+A+E L L+VSPFAPHLGEECW +LG+     ++F PW E+ E LCV +TVT+GVQVNGK+R +LE+ K A  + A+  A  +P V +F  GKE+KKFIY PG+II+ VV
Sbjct:   78 KRYDPAEIEPRWQKIWAEEKTFKTPERRDGKEKRYILDMFPYPSGAGLHVGHPEGYTASDVMARYYRMKDYDVLHPMGWDSFGLPAEQHAINTGTHPAVTTLENIANFKRQLSMLGFSYDWDREIATTDIRFVKWTQWIFLQLYKQGLATQSEVSVNWCPALGTVLANEEVINGKSERGDHPVKRLPLRQWVLKITEYADKLIDDLDGLNWPEGTLTAQRTWIGRSEGAEIEFNIQGHDESLSVFTTRPDTLMGVTYVCIAPEHPLAKTLPIDADHLEK-VSAYVEETSSKSDVDRTAAAA-KEKTGVDTGAKAIHPITGEEIPIWVADYVLGSYGSGAVMAVPAHDERDFAFADKFGLPRKEVVQAPPAKNNNXXXXXXXXXXK---ADVEDE-------KAQP--SAFTGEGKLVNSGE-FDGLKGDKAKAKIIEKLEAEGKGRRKIQYKLRDWIFSRQRYWGEPIPIYFPVNMADPNGDPRKGDEHTIDYQTPIPVPEEELPLQLPEMDNFQPGDDPQGCLARAADWRYFLKDGKWYARETNTMPQWAGSCWYYLRFIDPLNDQQPFSKQADDDWMPVDLYVGGQEHATLHLLYARFWHKVLYEAGLTKTKEPFQKLVHQGMILGTDGEKMSKSRGNVVNPDDVVNEHGADSLRLYEMFMGPLEATKPWQTSQVSGVVRFRDKVYALATRGDLQDTMDEKIEKAMHKCIKKVTEDVESMSFNTAISAMMVFTNTLQDAAKEGMVPRKAVETLALLVSPFAPHLGEECWKMLGHE--DSLAFHPWPEYDEALCVESTVTIGVQVNGKLRGQLEIEKDAPEDKAKEAAMAIPGVEQFAGGKEMKKFIYRPGKIINIVV 919          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A7S2RFM0_9STRA (Leucine--tRNA ligase n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2RFM0_9STRA)

HSP 1 Score: 1118 bits (2892), Expect = 0.000e+0
Identity = 554/869 (63.75%), Postives = 656/869 (75.49%), Query Frame = 1

Query:  595 TAGTKLYDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTEE--TVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMANA----EGDPRK-GDDHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAE--DVELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTF-EKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 3171
            TA +  Y  +++E +WQ  W E  TFKTPER   +PKK+VLDMFPYPSG+GLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ AI TG  P  TT  NID FRRQLKMLGFSYDWDRE+ATTD  YVKWTQWIFL+L+K GLA+QS   VNWCPALGTVLANEE+I+G SERG HPV R+PLRQW L+IT YAD+LEAGLEGL WP GT+AAQKQWIG+SEG +I FD+EG+EE   V VFTTR DTL GVTY+ LAPEHPL  ++ T +Q+  V++YV  ++SRSD+DR++    K+KTG  TGA A+HPL+ EKVP+W  DYVLGSYGTGAVMAVPAHDERDF FA  F L +K VV P                        EE ++           EAFT  GVA N  +  DGL+T +  +A+   L+E   G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+        + DP++ G +H I YD P+PVD SELPL LP ME F+PGDDP GCL R  DWRYFQ+DGKWFARETNTMPQWAGSCWYY R+ DNKN E   SK+ D++WMPVDLY+GG EHAVLHLLYARFWH+VL DLG+ + PEPF++LVHQGMILG DGEKMSKSRGNV+NPDDIV+A GADALRLYEMFMGPLEA KPWQT QV+GV RF++++Y + ++  E  DV+++ ET  +LHKTMKKV+EDIE ++FNTAISA+MVLTNHL +  E  PREA E L LMVSP APHLGEECW LLGN+    +++ PWVE+ E LCV NTVT+GVQVNGK R E+E+ K A  E A + A    +V    EGK++KK IYVPGRI++ V K
Sbjct:   77 TAASTNYPFEKVEKKWQDFWMENQTFKTPERDLSKPKKYVLDMFPYPSGTGLHVGHPEGYTASDVMARYWRMTGNDVLHPIGWDSFGLPAEQFAINTGAQPAETTTKNIDNFRRQLKMLGFSYDWDREIATTDVNYVKWTQWIFLQLYKKGLAQQSSVSVNWCPALGTVLANEEVINGLSERGDHPVERLPLRQWILKITDYADRLEAGLEGLDWPSGTMAAQKQWIGKSEGCNIDFDVEGSEEGDKVSVFTTRADTLFGVTYVTLAPEHPLISKITTADQKETVDDYVKKTSSRSDMDRTAS---KEKTGVFTGAYAIHPLTDEKVPIWVGDYVLGSYGTGAVMAVPAHDERDFEFASKFGLEVKWVVEPK--------------------KNPEEMSKE----------EAFTATGVATNSAD-FDGLTTAKTKKAITYNLKELNKGGPQITYKLRDWVFSRQRYWGEPIPIYFPVDFLEGVIPDDVDPKEDGCEHTIRYDQPIPVDESELPLELPHMEDFQPGDDPAGCLARAADWRYFQKDGKWFARETNTMPQWAGSCWYYFRYIDNKNDEEVISKKLDEEWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYTQHPEPFKKLVHQGMILGTDGEKMSKSRGNVVNPDDIVNAQGADALRLYEMFMGPLEAVKPWQTSQVSGVVRFQNKLYSVVKKAVENDDVKMDEETTKILHKTMKKVTEDIESMSFNTAISAMMVLTNHLQSLKENIPREAAEKLALMVSPIAPHLGEECWSLLGND--KSLAYHPWVEYDESLCVDNTVTMGVQVNGKKRGEIEILKDADQETAMSEAMKQGRVESQLEGKDIKKIIYVPGRILNIVAK 909          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A7S4I9K5_9STRA (Leucine--tRNA ligase n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4I9K5_9STRA)

HSP 1 Score: 1115 bits (2883), Expect = 0.000e+0
Identity = 551/862 (63.92%), Postives = 648/862 (75.17%), Query Frame = 1

Query:  613 YDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTE-ETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEM---ANAEGDPRKGDD--HKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAED--VELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTF-EKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 3171
            Y   QIE +WQ  WEE  TFKTPER   + KK+VLDMFPYPSG+GLHVGHPEGYTASDVM+RYWRM GFDVLHP+GWDSFGLPAEQ AI TG  P  TT  NI  F+RQLKMLGFSYDWDRE+ATTD EYVKWTQWIFL+L+K GLAEQS   VNWCPALGTVLANEE+I+G SERG HPV R+PLRQW L+IT YAD+LEAGL+GL WP GTL AQ+QWIG+S G +I FD++G E E +  FTTR DTLMGVTY+ LAPEHPL   V+T EQ+  V+ YV A++SRSDLDR++    K+KTG  TG  A+HP+SG+KVP+W  DYVLGSYGTGAVMAVPAHD+RDF FA  ++L +K VV P                    G A  +EA              FT NGVA N GE  DG++T  C + V K+LE    G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+     N E    KGDD  H I +D P+PV+ ++LPL LPEM+ F PGDDP GCL R  DWRYFQ+DGKWFARETNTMPQWAGSCWYY RF D KN +A +S + D+DWMPVDLYVGG EHAVLHLLYARFWH+VL DLG+ K  EPFQ+LVHQGMILG+DGEKMSKSRGNV+NPDDIVD  GADALRLYEMFMGPLEA KPWQTEQV+GV RF+++ Y + +  AE+   +++ ET  +LHKTMKKV+EDI+ ++FNTAISA+MVLTNHL T  +K P EA E L LMVSP APHLGEECW++LGN     +++  WVE+ EELCV NTV +GVQVNGKVR E+E+ K A  + A   A    +V    EGK++KK I+VPGRI++ V K
Sbjct:   72 YPFAQIEEKWQAFWEENNTFKTPERNPEKAKKYVLDMFPYPSGAGLHVGHPEGYTASDVMSRYWRMNGFDVLHPIGWDSFGLPAEQFAINTGARPADTTAKNIANFKRQLKMLGFSYDWDREIATTDVEYVKWTQWIFLQLYKTGLAEQSSVSVNWCPALGTVLANEEVINGLSERGDHPVTRLPLRQWLLKITDYADRLEAGLDGLDWPSGTLTAQQQWIGKSVGCNIEFDVDGLEGEKITAFTTRADTLMGVTYVTLAPEHPLVSSVSTAEQKDEVDAYVKATSSRSDLDRTAA---KEKTGVFTGGYAIHPISGDKVPIWVGDYVLGSYGTGAVMAVPAHDQRDFEFATKYNLDVKWVVKPK------------------KGDANTDEA--------------FTANGVAANSGE-FDGMTTTTCKKEVTKKLESMDKGGAKITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVNPEDTDPKGDDCEHTIRFDQPIPVEEADLPLRLPEMDDFTPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNDDAAFSPKVDEDWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTKHSEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDDIVDRQGADALRLYEMFMGPLEAVKPWQTEQVSGVVRFQNKFYNVVKAAAENGCTDMDEETTKILHKTMKKVTEDIDSMSFNTAISAMMVLTNHLQTLKDKVPVEAAEKLALMVSPLAPHLGEECWNMLGNE--DSLAYHQWVEYDEELCVDNTVKMGVQVNGKVRGEIEIPKDADQDLAMEEANKQSKVASQLEGKDIKKIIFVPGRILNIVAK 895          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A1Z5K7R9_FISSO (Leucine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K7R9_FISSO)

HSP 1 Score: 1113 bits (2879), Expect = 0.000e+0
Identity = 547/862 (63.46%), Postives = 658/862 (76.33%), Query Frame = 1

Query:  610 LYDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTE-ETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMAN----AEGDPRKGD-DHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGA--EDVELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTFEKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 3171
            ++  + +EA+WQ +W++  TF+TP+R   +PKK+VLDMFPYPSG+GLHVGHPEGYTASDVMARYWRMKG+DVLHP+GWDSFGLPAEQ+AI TG  P  TT  NI  F+RQL+MLGFSYDWDRELATTD EYVKWTQWIFL+LF+ GLAEQSE  VNWCPALGTVLANEE+I+G SERG HPV R+PLRQW L+IT+YAD+LE GL+GL+WP GTL+AQKQWIGRSEG  I F +EG + ET+ VFTTR DTLMGVTY+ LAPEHPL L V ++EQ+ AV  YV  ++SRSDLDR+S    K+K+G  TG  A+HP+SGE+VP+W  DYVLGSYGTGAVMAVPAHD+RDF FAQ + L IK VV PA                                A+ +  + AFT  GV VN GE  DGL+TE+  EAV K+LEE + G  +INY+LRDW+FSRQRYWGEPIPIYFP++       ++ DP+  D  H I YD P+PVD ++LPL LP ME F+PGD+P GCL R  DWRYFQ+DGKWFARETNTMPQWAGSCWYY RF D KN++  +SK+ D++WMPVDLYVGG EHAVLHLLYARFWH+VL DLG+   PEPFQ+LVHQGMILG+DGEKMSKSRGNV+NPDDIV + GADALR+YEMFMGPLEA KPWQT QVAGV RF ++ Y +    A  +  E+  ETL +++KTMKKV+EDIE ++FNTAISALMV  NHL + E  PREA + L LMVSPFAPHLGEECW++LG+     +++ PWV + E LCV  TVT+GVQVNGK R E+ ++K A    A A A  + +V    EGKE+KK IYVPGRI++ + K
Sbjct:   50 VFPFQHVEAKWQAYWQKHQTFRTPQRDLSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGYDVLHPIGWDSFGLPAEQYAIQTGAQPADTTAANIANFKRQLQMLGFSYDWDRELATTDVEYVKWTQWIFLQLFRKGLAEQSEVSVNWCPALGTVLANEEVINGLSERGDHPVERLPLRQWVLKITQYADRLEEGLQGLEWPAGTLSAQKQWIGRSEGCRIDFGIEGMDTETITVFTTRADTLMGVTYVTLAPEHPLVLSVTSEEQKDAVSAYVNEASSRSDLDRTSS---KEKSGVFTGGYALHPISGERVPIWVGDYVLGSYGTGAVMAVPAHDQRDFEFAQKYGLDIKWVVEPAD------------------------------HAELDQTV-AFTEYGVNVNSGE-FDGLTTEKAKEAVTKKLEELEKGGTQINYKLRDWVFSRQRYWGEPIPIYFPIDFPEGVDPSQQDPKLDDCQHTIRYDQPIPVDEADLPLKLPVMEDFQPGDNPAGCLARARDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPKNSKEAFSKKVDEEWMPVDLYVGGAEHAVLHLLYARFWHQVLFDLGYTTHPEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDDIVQSQGADALRMYEMFMGPLEAVKPWQTSQVAGVVRFMNKAYNIVVSAAATKHTEMGDETLRLMNKTMKKVTEDIENMSFNTAISALMVYANHLQSLETIPREAAKTLTLMVSPFAPHLGEECWNILGHT--ESLAYEPWVVFDEALCVDTTVTIGVQVNGKSRGEISIAKDADEATAVAAASGVDKVQVQLEGKEIKKIIYVPGRILNLIAK 874          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A448Z2U8_9STRA (Leucine--tRNA ligase n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z2U8_9STRA)

HSP 1 Score: 1112 bits (2875), Expect = 0.000e+0
Identity = 540/859 (62.86%), Postives = 650/859 (75.67%), Query Frame = 1

Query:  613 YDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTEETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMANA----EGDPRKGD-DHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAED--VELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTF-EKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFV 3165
            Y  +++E +WQK+WEE  TFKTPER   +PKK+VLDMFPYPSG+GLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ A+ TGT P  TT  NI  FRRQLKMLGFSYDWD+ELATTDT YVKWTQWIFL+L+K GLAEQSE  VNWC ALGTVLANEE+I+G SERG HPV R+PLRQW L+IT+YAD+LE+GL+GL WP GT+ +Q+QWIG+S G  I FD+EG++E+V+VFTTRPDTL+GVTY+ LAPEHPL   + T EQ+ +V++YV  ++SRSDLDR+S    K KTG  TGA   HP+SG++VPVW  DYVLGSYGTGAVMAVPAHD RDF FA+ F L +K VV P  G+                                    EAFT  GVAVN GE  DGL+T+ C +A+  +LEE   G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+          DP+  D +H I YD P+PVD ++LPL LP ME+FEPGDDP GCL R  DWRYF++DGKWFARETNTMPQWAGSCWYY RF D  NTE  +S+Q DKDWMPVDLY+GG EHAVLHLLYARFWH+VL DLG+ + PEPF++LVHQGMILG+DGEKMSKSRGNV+NPDD+V   GADALRLYEMFMGPLEA KPWQTEQV GV RF++R+Y +    A+    +++ ET  +LHKTMKKV+EDIE ++FNTAISA+MVLTNHL++  +K P EA E L LMVSP APHLGEECW +LG+     +++A WVE+ E LCV N VT+G QVNGK+R ++ +   A  E A + A+ +  V    +GK ++K IYVPGRI++ +
Sbjct:   67 YPFQEVEPKWQKYWEENNTFKTPERDTSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMTGHDVLHPIGWDSFGLPAEQFAVQTGTQPAETTGKNIANFRRQLKMLGFSYDWDKELATTDTAYVKWTQWIFLQLYKKGLAEQSEVSVNWCAALGTVLANEEVINGLSERGDHPVQRLPLRQWVLKITEYADRLESGLDGLNWPSGTMTSQQQWIGKSVGCEIVFDVEGSDESVKVFTTRPDTLLGVTYVTLAPEHPLVSSITTPEQKDSVDKYVLETSSRSDLDRTSS---KVKTGVFTGAYVTHPISGDRVPVWVGDYVLGSYGTGAVMAVPAHDSRDFEFAKKFDLDMKWVVEPKKGELNKD--------------------------------EAFTEPGVAVNSGE-FDGLATKNCKKAITNKLEEINKGGAQITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVDPTSQDPKNDDCEHTIRYDQPIPVDEADLPLELPHMENFEPGDDPAGCLARAKDWRYFEKDGKWFARETNTMPQWAGSCWYYFRFLDPSNTEEAFSQQMDKDWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYSEHPEPFEKLVHQGMILGSDGEKMSKSRGNVVNPDDVVSEQGADALRLYEMFMGPLEAVKPWQTEQVPGVVRFQNRLYNVVNAAAKSKTTDMDEETARILHKTMKKVTEDIESMSFNTAISAMMVLTNHLVSLGDKVPLEAAENLALMVSPLAPHLGEECWSILGHK--DSLAYATWVEYDEALCVDNEVTIGCQVNGKMRGQITIPAEADEETAVSAAKEMENVQSQIDGKTIRKIIYVPGRILNII 887          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A7S2XSK2_9STRA (Leucine--tRNA ligase n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XSK2_9STRA)

HSP 1 Score: 1110 bits (2871), Expect = 0.000e+0
Identity = 547/861 (63.53%), Postives = 656/861 (76.19%), Query Frame = 1

Query:  613 YDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTE-ETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMAN----AEGDPRK-GDDHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAED--VELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTF-EKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVV 3168
            Y   +IE RWQ  W+++ TFKTP R   +PKK+VLDMFPYPSG+GLHVGHPEGYTASDVMARYWRMKG+DVLHP+GWDSFGLPAEQ AI TGT P VTT +NI  F+RQLKMLGFSYDWDRELATTD E+VKWTQWIFL+LFK GLAEQS   VNWCP LGTVLANEE+I+G SERG  PV+R+PLRQW L+IT YAD+LE GLEGL WP GT+AAQKQWIG+S G++I F ++G + E + VFTTR DTLMGVTY+ LAPEHPL L + ++EQ+  VE YV  ++SRSDLDR++    K+KTG  TG   +HP+SGEKVP+W  DYVLGSYGTGAVMAVPAHD RDF FAQ F L IK VV P+  K                              + +   EAFT +G  +N GE  DGL+T +  +A+ ++L E   G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+       ++ DP++ G +H I YD P PVD +ELPLTLPE++ F+PGDDP GCL R  DWRYFQ+DGKWFARETNTMPQWAGSCWYYLR+ D  N+   +SKQAD DWMPVDLYVGG EHAVLHLLYARFWH+V+ DLG+ + PEPFQ+LVHQGMILG+DGEKMSKSRGNV+NPD+IVDA GADALRLYEMFMGPLEA KPWQT QV+GV RF++++Y +     E+   E++ ET  +LHKTMKKVSEDI+ L FNTAISALMVLTNHL++  +K P EA E L LMVSPFAPHLGEECW LLG+     +++ PWVE+ E LC+   VT+GVQVNGKVR E+ ++K A  + A + A    +V    +GK++KK IYVPGRI++F+V
Sbjct:   84 YPFAEIEKRWQAKWQDEQTFKTPIRNPDKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMKGYDVLHPIGWDSFGLPAEQFAINTGTKPAVTTASNIANFKRQLKMLGFSYDWDRELATTDVEFVKWTQWIFLQLFKKGLAEQSSVSVNWCPELGTVLANEEVINGLSERGDFPVIRLPLRQWLLKITDYADRLEEGLEGLDWPSGTMAAQKQWIGKSIGSNIDFKVDGLDTEKISVFTTRADTLMGVTYVTLAPEHPLVLSICSEEQKEEVEAYVKETSSRSDLDRTAA---KEKTGVFTGGYGIHPISGEKVPIWVGDYVLGSYGTGAVMAVPAHDVRDFEFAQKFGLEIKWVVQPSQTK------------------------------ELKSTEEAFTPHGACINSGE-FDGLTTAKAKKAITRKLAEMDMGGAKITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVNPSDADPKEDGCEHTIRYDQPFPVDEAELPLTLPEIDDFKPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYLRYLDPTNSGEAFSKQADADWMPVDLYVGGAEHAVLHLLYARFWHQVMFDLGYTQHPEPFQKLVHQGMILGSDGEKMSKSRGNVVNPDEIVDAQGADALRLYEMFMGPLEAVKPWQTSQVSGVVRFQNKLYTVVNTAIENQVTEMDDETTRILHKTMKKVSEDIDTLGFNTAISALMVLTNHLVSLKDKVPLEAAEKLALMVSPFAPHLGEECWKLLGHT--EMLAYHPWVEYDESLCIDTDVTIGVQVNGKVRGEIFIAKDADQDLAMSEARKQERVAFQLDGKDIKKVIYVPGRILNFIV 908          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: W7TSV9_9STRA (Leucine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TSV9_9STRA)

HSP 1 Score: 1108 bits (2865), Expect = 0.000e+0
Identity = 552/863 (63.96%), Postives = 649/863 (75.20%), Query Frame = 1

Query:  613 YDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLE---GTEETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMANAE-GDPRKGDDHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQR-DGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRG--AEDVE-LESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMT--FEKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 3171
            Y+H+ IE +WQ++W E+ TFK   R  G+ KK+VLDMFPYPSGSGLHVGHPEGYTASDVMARYWRM   DVLHPMGWDSFGLPAEQHAI TGTHP VTTY NI  FRRQLK LGFSYDW RELATTD +YV+WTQWIFL+L+K GLAEQSE  VNWCPALGTVLANEEIIDGKSERG HPV R+PLRQW L+ITKYA++LE  L+GL WPEGT+ AQK WIGRSEGA + F L        +VEVFTTRPDTLMGVTY+VLAPEHPL  +V ++E+RAAV  YV  +A +SDLDR++ GK K KTG DTGA  VHPLSGE++P+W ADYVL  YGTGAVMAVPAHDERD AFA  F LPIK+VV P  G                     EEEA              F G G  VN G  LDG  T +  + VV++LE+ + G   +NYRLRDW+FSRQRYWGEPIPIYFPV+MA+ + GDPRK D H I +D P+PV+ +ELPL LP+M++F PG+DPQGCL R  DWR+F++ DGKW+ARETNTMPQWAGSCWYYLRF+D  N +  W ++AD+ W+PVDLYVGGQEHAVLHLLYARFWHK L DLG  K  EPF +LVHQGMILG DGEKMSKSRGNV+NPDD+V   G DALRLYEMFMGPLEA KPWQT QVAGV RFRD+V+QL R     +  E ++ ETL ++HKT+KKV++D+E L+FNTAIS +M+  NHL +   E  PR A+E LVLMVSPFAPHLGEECW++LG++    ++  PW  ++EEL V + V LGVQVNGK R E+ +   A+  D  A A    +V  F +GK V K IYVPGRI++ V K
Sbjct:   75 YEHEVIEPKWQRYWAERETFKAIRRP-GKGKKYVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMCDLDVLHPMGWDSFGLPAEQHAINTGTHPGVTTYENIANFRRQLKSLGFSYDWSRELATTDWDYVRWTQWIFLQLYKKGLAEQSEVLVNWCPALGTVLANEEIIDGKSERGDHPVERLPLRQWVLKITKYAEELEEDLKGLDWPEGTMTAQKLWIGRSEGARVMFPLACSGAIPGSVEVFTTRPDTLMGVTYVVLAPEHPLVERVTSEERRAAVRAYVREAAGKSDLDRTATGKDKGKTGVDTGAKVVHPLSGEELPIWVADYVLWGYGTGAVMAVPAHDERDHAFATIFGLPIKQVVAPDAGSDALP---------------VEEEA--------------FVGEGQVVNSGPLLDGQPTSEAKKVVVEELEKNERGAAVVNYRLRDWVFSRQRYWGEPIPIYFPVDMADHDTGDPRKDDPHTIRFDQPIPVEEAELPLKLPDMDNFAPGNDPQGCLARAKDWRFFKKSDGKWYARETNTMPQWAGSCWYYLRFSDPTNLKRAWGEEADEQWLPVDLYVGGQEHAVLHLLYARFWHKFLFDLGLTKHREPFLKLVHQGMILGTDGEKMSKSRGNVVNPDDVVKEVGGDALRLYEMFMGPLEAVKPWQTAQVAGVVRFRDKVFQLVRNREMTDSTEAMDDETLRLMHKTIKKVTQDVETLSFNTAISTMMIFANHLASRKTETLPRAAVENLVLMVSPFAPHLGEECWEMLGHD--QSLACHPWPRYQEELTVDDVVKLGVQVNGKTRGEVSVPADATEADVMAAAMEQEKVASFLDGKRVVKVIYVPGRILNIVAK 905          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A7S4AJT1_9STRA (Leucine--tRNA ligase n=2 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4AJT1_9STRA)

HSP 1 Score: 1103 bits (2854), Expect = 0.000e+0
Identity = 540/860 (62.79%), Postives = 646/860 (75.12%), Query Frame = 1

Query:  613 YDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQWPEGTLAAQKQWIGRSEGASITFDLEGTE-ETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMAN----AEGDPRKGD-DHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDV--ELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTF-EKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFV 3165
            Y   ++E +WQK+WEE  TFKTPER   +PKK+VLDMFPYPSG+GLHVGHPEGYTASDVMARYWRM G DVLHP+GWDSFGLPAEQ+A+ TGT P  TT  NI  F RQLKMLGFSYDWD+ELATTDT YVKWTQWIFL+L+K GLAEQSE  VNWCPALGTVLANEE+I+G SERG HPV R+PLRQW L+IT+YAD+LEAGL+GL WP GT+ +Q+ WIG+S G  I FD+EG   ETV+VFTTR DTL+GVTY+ LAPEHPL   + T EQ+  V+ YVA ++SRSDLDR+S    K+KTG  TGA   HP+SG++VPVW  DYVLGSYGTGAVMAVPAHD RDF FA+ F L +K VV P                    G   +EEA              FT  GVAVN GE  DGL+T+ C +A+  +LEE   G  +I Y+LRDW+FSRQRYWGEPIPIYFPV+       A  DP+  D +H I YD  +PVD ++LPL LP ME+FEPGDDP GCL R  DWRYFQ+DGKWFARETNTMPQWAGSCWYY RF D  N +  +SKQ DKDWMPVDLY+GG EHAVLHLLYARFWH+VL DLG+ + PEPF++LVHQGMILG DGEKMSKSRGNV+NPDD+V   GADALR+YEMFMGPLEA KPWQT QV GV RF++R+Y +    A     E++ ET  +LHKTMKKV+EDIE ++FNTAISA+MVLTNHL++  +K P EA E L LMVSP APHLGEECW++LGN     +++APWVE+ E LCV + +T+GVQVNGK+R ++ ++  A  E A + A+ +  V    +GK ++K IY+ GRI++ +
Sbjct:   67 YPFAEVEPKWQKYWEENNTFKTPERDTSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDVMARYWRMTGHDVLHPIGWDSFGLPAEQYAVQTGTQPAATTKKNIANFMRQLKMLGFSYDWDKELATTDTNYVKWTQWIFLQLYKKGLAEQSEVSVNWCPALGTVLANEEVINGLSERGDHPVKRLPLRQWVLKITEYADRLEAGLDGLNWPSGTMTSQQAWIGKSIGCEIDFDVEGDSGETVKVFTTRADTLLGVTYVTLAPEHPLVSSITTPEQKEVVDSYVATTSSRSDLDRTSS---KEKTGVFTGAFVTHPISGDRVPVWVGDYVLGSYGTGAVMAVPAHDTRDFEFAKKFELDVKWVVEPK------------------KGELNKEEA--------------FTAPGVAVNSGE-FDGLATKNCKKAITSKLEEINKGGAQITYKLRDWVFSRQRYWGEPIPIYFPVDFPEGVDPASVDPKNDDCEHTILYDQAIPVDEADLPLELPYMENFEPGDDPAGCLARAKDWRYFQKDGKWFARETNTMPQWAGSCWYYFRFLDPNNNDEAFSKQMDKDWMPVDLYIGGAEHAVLHLLYARFWHQVLFDLGYSEHPEPFEKLVHQGMILGTDGEKMSKSRGNVVNPDDVVGENGADALRMYEMFMGPLEAVKPWQTGQVPGVVRFQNRLYNVVNAAARSQTNEMDEETTRILHKTMKKVTEDIELMSFNTAISAMMVLTNHLVSLGDKVPLEAAEKLALMVSPLAPHLGEECWNILGNE--ESLAYAPWVEYDESLCVDDEITMGVQVNGKMRGKITIAADADEEAAVSAAKEMNTVQSQIDGKTIRKIIYIQGRILNII 888          

BLAST of mRNA_P-fluviatile_contig13.1737.1 vs. uniprot
Match: A0A1E7FAE0_9STRA (Leucine--tRNA ligase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FAE0_9STRA)

HSP 1 Score: 1101 bits (2848), Expect = 0.000e+0
Identity = 543/864 (62.85%), Postives = 652/864 (75.46%), Query Frame = 1

Query:  613 YDHKQIEARWQKHWEEKGTFKTPERTEGRPKKFVLDMFPYPSGSGLHVGHPEGYTASDVMARYWRMKGFDVLHPMGWDSFGLPAEQHAILTGTHPKVTTYTNIDTFRRQLKMLGFSYDWDRELATTDTEYVKWTQWIFLELFKAGLAEQSEARVNWCPALGTVLANEEIIDGKSERGAHPVVRMPLRQWALRITKYADQLEAGLEGLQ--WPEGTLAAQKQWIGRSEGASITFDLEGTEETVEVFTTRPDTLMGVTYIVLAPEHPLTLQVATDEQRAAVEEYVAASASRSDLDRSSMGKGKKKTGTDTGAVAVHPLSGEKVPVWTADYVLGSYGTGAVMAVPAHDERDFAFAQAFHLPIKRVVTPAGGKGXXXXXXXXXXXXXXXGSAAEEEARAGIEAKAEPVLEAFTGNGVAVNCGEGLDGLSTEQCAEAVVKQLEERQHGRKEINYRLRDWIFSRQRYWGEPIPIYFPVEMANAEGDPRKGD------DHKICYDHPMPVDVSELPLTLPEMESFEPGDDPQGCLTRVPDWRYFQRDGKWFARETNTMPQWAGSCWYYLRFTDNKNTEAGWSKQADKDWMPVDLYVGGQEHAVLHLLYARFWHKVLHDLGHVKDPEPFQRLVHQGMILGADGEKMSKSRGNVINPDDIVDAYGADALRLYEMFMGPLEATKPWQTEQVAGVTRFRDRVYQLARRGAEDV--ELESETLTMLHKTMKKVSEDIEELAFNTAISALMVLTNHLMTF-EKPPREALEALVLMVSPFAPHLGEECWDLLGNNTGSGVSFAPWVEWKEELCVSNTVTLGVQVNGKVRAELELSKTASSEDARALAEVLPQVVKFTEGKEVKKFIYVPGRIISFVVK 3171
            Y   Q+E +WQ +WEE  TFKTP R   +PKK+VLDMFPYPSG+GLHVGHPEGYTASD+MARYWRM G DVLHP+GWDSFGLPAEQ+A+ TGT P VTT  NI TF+RQLK LGFSYDWDRELATTDT YVKWTQWIFL+L+K GLAEQSE  VNWCPALGTVL+NEE+I+G SERG HPV R+PLRQW LRITKYAD+LEAGL  LQ  WP GTL +Q+QWIG+S G  I FD E  +E+V+VFTTRPDT+MGVTY+ LAPEHPL   + T EQ+  V+ YV  ++SRSD+DR+S    K+KTG  TG    HPLSG++VPVW  DYVLGSYGTGAVMAVP HD RDF FAQ F L IK VV P  G+                   ++EE              AFT  GV +N G+  DGLST++C +AV  +LEE + G  +I Y+LRDW+FSRQRYWGEPIPI+FPV+    + DP   D      +H I YD P+PV+ ++LPL LP M++FEPGDDP GCL R  DWRYF++DGKWFARETNTMPQWAGSCWYY RF D +N E  +SKQ DKDWMPVDLYVGG EHAVLHLLYARFWH+VL+DLG+ + PEPFQ LVHQGMILG DGEKMSKSR NV+NPDD+V   GADALR+YEMFMGPLEA KPWQT QV GV RF++R+Y +    A+    +++ ET  +LHKTMKKVSEDIE ++FNTAISALMVLTNHL++  +K P EA E+L LMVSPFAPHLGEECW +LG+ TG+ +++ PWV+++E+LCV + + +GVQVNGK R ++ +   A  E A + A  +  V +  EGK++KK IYVPGRI++ + K
Sbjct:   52 YPFAQVETKWQAYWEENNTFKTPVRDTSKPKKYVLDMFPYPSGAGLHVGHPEGYTASDIMARYWRMTGHDVLHPIGWDSFGLPAEQYAVQTGTQPGVTTKKNIATFKRQLKSLGFSYDWDRELATTDTNYVKWTQWIFLQLYKEGLAEQSEVSVNWCPALGTVLSNEEVINGLSERGDHPVTRLPLRQWLLRITKYADRLEAGLGDLQGQWPAGTLTSQQQWIGKSTGCEIVFDAESIDESVKVFTTRPDTVMGVTYVTLAPEHPLVSSMTTPEQKETVDSYVKKTSSRSDMDRTSS---KEKTGVFTGGYVTHPLSGDRVPVWVGDYVLGSYGTGAVMAVPGHDTRDFEFAQKFDLGIKWVVKPKDGE-------------------SDEEV-------------AFTEPGVVMNSGD-FDGLSTDECKKAVTSKLEEIKKGGAQITYKLRDWVFSRQRYWGEPIPIFFPVDFPE-DVDPSSQDLINEECEHTIRYDQPIPVEEADLPLELPYMKNFEPGDDPAGCLARAKDWRYFEKDGKWFARETNTMPQWAGSCWYYFRFLDPENKEEAFSKQLDKDWMPVDLYVGGAEHAVLHLLYARFWHQVLYDLGYTEHPEPFQNLVHQGMILGTDGEKMSKSRNNVVNPDDVVGEQGADALRMYEMFMGPLEAVKPWQTAQVPGVVRFQNRLYNVVNAAAKSKTEDMDEETTRLLHKTMKKVSEDIESMSFNTAISALMVLTNHLVSLGDKVPLEAAESLALMVSPFAPHLGEECWSILGH-TGT-LAYHPWVKFEEKLCVDDVINMGVQVNGKSRGQVTIPADADEETALSAAMDVGPVQQQLEGKDIKKIIYVPGRILNIIAK 876