mRNA_P-fluviatile_contig13.1723.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A6H5L2M1_9PHAE (Isoleucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5L2M1_9PHAE)

HSP 1 Score: 1866 bits (4833), Expect = 0.000e+0
Identity = 956/1103 (86.67%), Postives = 1009/1103 (91.48%), Query Frame = 2

Query:   23 MRLPWVGSLALLTPALGFVAQSIFVVRGSPSMLQAFVWPSAGVVRAVSSSRGWRRQQSLRLQQLQRAAPLLQQSLANGGMRMXXXXXXXXXXXXXMAAKQEMAKK-PESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRVLEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESGEPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPEKHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHARHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQ---VLIYCGDESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWYQCGSVGSHKHHPTLCSRCNGIVEGLGLPPXXXXXXXXXXXXAEAAAVSAA 3319
            MR PWVGSLALL PA GFVAQS+ VVRGSP MLQAFV PSAG VRA +SSR WRR+ S+RLQQLQR A L Q     G +RM       XXXXXX           PESYYK+TVILPQT FEQRANAVKREPELQAFWDEERIY+RVL+DN GEK+VLHDGPPYANGDLHIGHALNKILKDFINRYQML+GRKV +VPGWDCHGLPIELKVLQSMKSKER GMTPV+LRKKAAEFAR+TVSKQS SFRRYGVWGDFE+PYLTLQPEYEAAQIEVFGKMFT+GHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEV EATPAL ++M S GGLAV+VWTTTPWTLPANLAVAVSETI+YSVVEHASLG RKLLVATDLVG VSAKIGLEEGD+LKV++++TG+DL+G KYQHPLCDRVSEVV+GGDYITTE GTGLVHTAPGHGQEDYVTG KYGLPLLSPVDDAGKFTAEAG+RFKGL+VLKEGNEEVLVALEEAGGLLA+EAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRD ALSAIEKV+WIPEVGQRRIT+MVEGR+DWCISRQRSWGLPIPVFY++ESGEPMLT ESIEHIR IVAEHGSD+WWEME ADLLPPSL+ EADQWRRGTDTMDVWFDSGSSWAGVAKAREEL YPADLYLEGSDQHRGWFQSSLLTSVAANG APYKTVLTHGFVLDEKGYKMSKSLGNTLDP EVIEGG NKKQKP YGADVLRLWVSSVDYSGDVCVGD IIKQ SE+YRKLRNTLRYL+GSLFDFDPEK +VPYE+LPSLDKYMLG+LSSVLEEVE A+DGYQFYKASQALQRFAT DLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLS VMAPILPHMAEDVWQTLPFKPEGG+RSIFQAGWAT RHA+HE +KWGRLRTLR DVNKALEQAR AK+LGSSLEGQ   V IYC DE +KAEL+S+LGDETLKREPELSNGVDDLRFVLMVSKINLV SAEAVDSACAEGMTVAA DSESGC VGVS APGNKCERCWY C SVGSH  HP+LCSRC+G+VEGLGL PXXXXXX       E AAVSAA
Sbjct:    1 MRSPWVGSLALLAPAHGFVAQSV-VVRGSPKMLQAFVRPSAGGVRA-ASSRTWRRE-SVRLQQLQRVARLPQPVAVVGDVRMMASGAGAXXXXXXXXXXXXXXXXXPESYYKKTVILPQTGFEQRANAVKREPELQAFWDEERIYERVLDDNTGEKFVLHDGPPYANGDLHIGHALNKILKDFINRYQMLRGRKVGYVPGWDCHGLPIELKVLQSMKSKERRGMTPVQLRKKAAEFARNTVSKQSASFRRYGVWGDFEKPYLTLQPEYEAAQIEVFGKMFTEGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVKEATPALEKYMGSGGGLAVSVWTTTPWTLPANLAVAVSETIQYSVVEHASLGDRKLLVATDLVGAVSAKIGLEEGDTLKVVETFTGKDLIGTKYQHPLCDRVSEVVIGGDYITTETGTGLVHTAPGHGQEDYVTGQKYGLPLLSPVDDAGKFTAEAGDRFKGLNVLKEGNEEVLVALEEAGGLLAREAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDTALSAIEKVQWIPEVGQRRITTMVEGRSDWCISRQRSWGLPIPVFYNIESGEPMLTTESIEHIRAIVAEHGSDSWWEMEVADLLPPSLQGEADQWRRGTDTMDVWFDSGSSWAGVAKAREELDYPADLYLEGSDQHRGWFQSSLLTSVAANGHAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPTEVIEGGNNKKQKPAYGADVLRLWVSSVDYSGDVCVGDQIIKQASESYRKLRNTLRYLTGSLFDFDPEKDSVPYEDLPSLDKYMLGMLSSVLEEVENAFDGYQFYKASQALQRFATTDLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSTVMAPILPHMAEDVWQTLPFKPEGGHRSIFQAGWATERHAQHEAEKWGRLRTLRNDVNKALEQARGAKVLGSSLEGQASLVFIYCSDEGLKAELESLLGDETLKREPELSNGVDDLRFVLMVSKINLVSSAEAVDSACAEGMTVAAKDSESGCVVGVSRAPGNKCERCWYHCDSVGSHDDHPSLCSRCHGVVEGLGLAPXXXXXXAVE---TEPAAVSAA 1097          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A835YNF4_9STRA (Isoleucine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNF4_9STRA)

HSP 1 Score: 1318 bits (3411), Expect = 0.000e+0
Identity = 657/978 (67.18%), Postives = 777/978 (79.45%), Query Frame = 2

Query:  329 AKKPESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRVLEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESGEPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPEKHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHARHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGD-ETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACA--EGMTVAANDSESGCFVGVSLAPGN--KCERCWYQCGSVGSHKHHPTLCSRCNGIVEGLG 3247
            AKKP+S YK +VILPQT F QRANAV REPELQ +W E R+Y+++ E N G+ YVLHDGPPYANGDLHIGHALNKILKDFINRYQ+L+G++  FVPGWDCHGLPIELKVLQSMKS ERAG+T ++LR++AA FA +TV +Q ++F+RYGVW  ++ PYLTLQPEYEAAQIEVFGKM   GHI+RGRKPVNWSPSSRTALAEAELEYP  H STS+YAAF+V + + ALA    +   + VA+WTTTPWT+PANLAVAV+E ++Y +V HA LG ++L+VA +L+  +SAK+G  EG+  +V+ + +G DL G  YQHPL +R S+VV+GGDYITTE GTGLVHTAPGHGQEDY TGLKYGLPLLSPVDDAG FT EAGERF+GLSVLKEGNE VL A+ E+G LL  EAY HKYPYDWRTKKPTIFRATDQ  A+    R +       V+WIPEVG+ RIT MV+GR+DWCISRQRSWG+PIPVFYH E+GEP+LT+ESI H+  IV E G+DAW+ M  A+LLP S   +A+ W +GTDTMDVWFDSGSSWAGVA  RE L YPAD+YLEGSDQHRGWFQSSLLTSVAANG AP+KTVLTHGFVLDEKG+KMSKSLGNT+DPKEVIEGG N+K KP +GADVLRLWVS+VDYSGDV VGD+I+KQV E+YRKLRNT RYL+G+LFDFDP    + Y++LPSLDK++LG LS V+ EVE AY+ YQFY+ASQA+QRFA  DLSNFYLD AKDRLYIS     RRRSCQTVLH +L++LSK MAPILPHMAED+WQTLPF      +SIFQ GW       HE   W RLR LR DVNKALE AR AK+LG+SLE  V I+C D  + A L +M GD +T+ + P  +NGVDDLRFVLMVS++ L+ S +AV +A A  E  T A    ESGC V V+ A G   KC+RCWYQC +VG+   HP +C+RC  ++   G
Sbjct:   32 AKKPDSPYKTSVILPQTSFNQRANAVVREPELQQYWAENRVYEQLAESNPGDVYVLHDGPPYANGDLHIGHALNKILKDFINRYQLLQGKRARFVPGWDCHGLPIELKVLQSMKSGERAGLTTLQLRERAAAFATATVERQRDAFKRYGVWALWDEPYLTLQPEYEAAQIEVFGKMLDAGHIYRGRKPVNWSPSSRTALAEAELEYPPDHISTSMYAAFKVVQPSAALAVHAAAGADVRVAIWTTTPWTIPANLAVAVNEKLDYCLVTHAVLGDKQLVVAKELIPALSAKLGAPEGEQFEVVATLSGADLAGTTYQHPLYERQSQVVIGGDYITTETGTGLVHTAPGHGQEDYQTGLKYGLPLLSPVDDAGCFTEEAGERFQGLSVLKEGNEAVLEAMRESGALLLSEAYNHKYPYDWRTKKPTIFRATDQVSAAAAAARASCCRCA--VQWIPEVGKNRITGMVQGRSDWCISRQRSWGVPIPVFYHKETGEPLLTQESIAHVVDIVREKGTDAWFRMSVAELLPLSHAADAEHWDKGTDTMDVWFDSGSSWAGVANGREGLRYPADMYLEGSDQHRGWFQSSLLTSVAANGHAPFKTVLTHGFVLDEKGFKMSKSLGNTVDPKEVIEGGANQKLKPAWGADVLRLWVSTVDYSGDVPVGDNILKQVGESYRKLRNTARYLTGNLFDFDPAADAIAYDDLPSLDKWVLGRLSDVMREVEEAYETYQFYRASQAIQRFAVQDLSNFYLDTAKDRLYISEPGAARRRSCQTVLHHVLINLSKAMAPILPHMAEDIWQTLPFPV--AEKSIFQTGWVDASFPSHEAAVWDRLRLLRADVNKALEAARTAKLLGASLEAAVFIHCEDSELLATLTAMKGDADTITQHPVSTNGVDDLRFVLMVSQVELLASRDAVAAAAAAPELHTAAG---ESGCAVSVARAGGGRTKCDRCWYQCSTVGASHAHPGVCARCASVLAATG 1002          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A7S1ZYZ0_9STRA (Isoleucine--tRNA ligase n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZYZ0_9STRA)

HSP 1 Score: 1271 bits (3290), Expect = 0.000e+0
Identity = 632/981 (64.42%), Postives = 764/981 (77.88%), Query Frame = 2

Query:  332 KKPESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQR---VLEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESG-EPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPE----KHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHA--RHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWY---QCGSVGSHKHHPTLCSRCNGIV 3235
            KK ++ Y  T++LP+T F QRANAV REPELQ +W +  +Y +   V E+    ++VLHDGPPYANGDLHIGHALNK+LKDFINR Q+LKG++V +VPGWDCHGLPIELKVLQ+MKSKER  +TP+ LR+KAA FA+ TV+KQSESF+RYGV+GDFE PYLTLQP++EA QI+VFG+M+ +G+IFRGRKPV+WSPSSRTALAEAELEYP GH S S+Y AF+V   + AL EF  +DGGL VA+WTTTPWT+PANLA+AV+  +EY+VVEH   G  +LLVA DL  +++ K  L EG+ L +L +  G +++G  Y HPL DR S V+ GGDYITTE+GTGLVHTAPGHGQEDY+TGLK GL LLSPVDD G+FT EAGERF G++VLKEGNEEV+ AL+E G LL  E Y HKYPYDWRTKKPTIFRATDQWFASV+ FRD AL AI+ V+W+PEVG+ RITS V GR DWCISRQRSWG+PIPVFY  E+G E +L E +++H++ I +EHGSDAWW+M+  DLLP   +EEA++W++GTDTMDVWFDSGSSWAGVA AR+EL YPADLYLEGSDQHRGWFQSSLLTSVAA G APYKTVLTHGFVLDEKGYKMSKSLGN +DP +VIEGG NKKQ+P YGADVLRLWV++VDYSGDVC+G++IIKQ  E YRKLRNT RYL G+L D+ PE     + VPYE+LPS+DK+MLG LS++L EV+ A D YQF +A+Q + RFATADLSNFYLD AKDRLYISS DD RRRSCQTVLH  L   SK +APILPHMAED+WQ LP+K      S+F+ GW     +   H++++W  +R LR DVNK LE ARA K++G+SL+    +Y  DES +  L  +  DE L   P  +NG+DDLR  LM+S++ LVDSA+ V  AC E     +  + SGC VGV  A G KC RCW+   Q G++G    H  LC RC   V
Sbjct:   67 KKEKNVYAPTILLPETPFSQRANAVVREPELQQYWKDSDLYNKLSSVAEEAKAPRFVLHDGPPYANGDLHIGHALNKLLKDFINRKQLLKGKQVHYVPGWDCHGLPIELKVLQTMKSKERQALTPITLREKAASFAKETVAKQSESFQRYGVFGDFENPYLTLQPQFEAEQIKVFGEMYKKGYIFRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYVAFDVDTPSDALKEFHDADGGLRVAIWTTTPWTMPANLAIAVNPDLEYAVVEHEKTG--RLLVAADLAESLATKFELPEGEKLSILATMKGSEIIGTTYSHPLYDRKSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKAGLDLLSPVDDLGRFTIEAGERFAGMNVLKEGNEEVVTALQETGSLLRAEDYGHKYPYDWRTKKPTIFRATDQWFASVEGFRDAALDAIDTVRWVPEVGKNRITSFVSGRGDWCISRQRSWGVPIPVFYDEETGNEVLLDEHTLDHVQKIFSEHGSDAWWKMDEKDLLPEMYKEEAEKWKKGTDTMDVWFDSGSSWAGVASARDELQYPADLYLEGSDQHRGWFQSSLLTSVAAQGQAPYKTVLTHGFVLDEKGYKMSKSLGNVIDPLKVIEGGNNKKQEPAYGADVLRLWVATVDYSGDVCIGNNIIKQTFETYRKLRNTARYLIGNLNDYVPEGQDNSNAVPYEDLPSMDKWMLGRLSALLREVDEATDDYQFSRATQEVLRFATADLSNFYLDVAKDRLYISSIDDPRRRSCQTVLHACLEGFSKAIAPILPHMAEDIWQNLPYKKPT--ESVFEGGWPGHLMSFPEHDEEQWTVVRNLRDDVNKVLESARADKLVGASLDAAAYVYAPDESTRDILAKLESDEHLVYPPVKTNGIDDLRTALMISQVKLVDSADLVSEACDESYV--SKGAFSGCTVGVKKAEGKKCGRCWFYDEQVGNLGLP--HDDLCQRCGHAV 1039          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A7S4II32_9STRA (Isoleucine--tRNA ligase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4II32_9STRA)

HSP 1 Score: 1264 bits (3270), Expect = 0.000e+0
Identity = 626/982 (63.75%), Postives = 767/982 (78.11%), Query Frame = 2

Query:  341 ESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRV---LEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMC--SDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESG-EPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPEKH----TVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHARHEDD--KWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWYQCGSVGS----HKHHPTLCSRCNGIVE 3238
            ++ Y  T++LP+T F QRANA KREPELQ FW    +Y R+    E+   +++VLHDGPPYANGDLHIGHALNK+LKDFINR+Q+LKG++V +VPGWDCHGLPIELKVLQS+KSKER G+TP+ LR+KAA+FA++TV KQSESF+RYGV GDF++PYLTLQP++EAAQI VFG+MF +G+IFRGRKPV+WSPSSRTALAEAELEYP GH S S+Y AFEV E +  L  +    SD  L VAVWTTTPWT+PANLAVAV+  +EYSVVEH   G  +LLVATDL  ++++K  L EG++  VL +  G D+VG  Y HPL  R S V+ GGDYITTE+GTGLVHTAPGHGQEDY+TGLK GL LLSPVDDAG+FT EAGE+F+G++VLKEGN+ ++ ALEE   LL  E Y HKYPYDWRTKKPTIFRATDQWFASV+ FRD AL A++ VKWIP+VG+ RITS V GR DWCISRQRSWG+PIPVFY  E+G E +L E+++EHI+ + +EHGSD WW M+  +LLP   R +A +W++GTDTMDVWFDSGSSWAGVA++R+ELAYPADLYLEGSDQHRGWFQSSLLTSVAA G APYK VLTHGFVLDEKG+KMSKSLGN +DP +VIEGG NKKQ+P YGADVLRLWV+SVDY+GDVC+G++IIKQ  E+YRKLRNT RYL G+L D+ PE       VPY++LPS+DK+MLG LSSVL EV+ A D YQF +A+Q + RFA+ADLSNFYLD AKDRLYIS  DDFRRRSCQTVL   L   +K +AP+LPHMAED+WQ +P++ +G   S+F+ G+  G     E D  +W  +R LR DVNKALE ARA K++G+SL+    ++C DE ++  L  ++GD  L   P  SNGVDDLR  LM+S++NLVDSAE V   C +   +     +SGC VGV+ A G+KC RCW+   +VG+     +  P LC RC+  ++
Sbjct:   31 KNVYAPTILLPETSFSQRANAAKREPELQQFWKSTDLYGRLSSSAEEAGADRFVLHDGPPYANGDLHIGHALNKLLKDFINRHQILKGKQVHYVPGWDCHGLPIELKVLQSLKSKERQGLTPITLREKAADFAKATVEKQSESFQRYGVIGDFDKPYLTLQPQFEAAQIGVFGEMFRRGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSIYVAFEVEEPSDKLRPYHGERSDDRLKVAVWTTTPWTMPANLAVAVNPELEYSVVEHERTG--RLLVATDLANSLASKFSLPEGEAFAVLATMRGEDVVGTSYNHPLYPRTSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLDLLSPVDDAGRFTKEAGEKFEGMNVLKEGNDAIVEALEETKSLLLAEDYGHKYPYDWRTKKPTIFRATDQWFASVEGFRDEALEAVDTVKWIPDVGKNRITSFVVGRGDWCISRQRSWGVPIPVFYDRETGNEVLLDEKTLEHIQSLFSEHGSDCWWTMDEEELLPEEYRADASKWKKGTDTMDVWFDSGSSWAGVARSRDELAYPADLYLEGSDQHRGWFQSSLLTSVAAEGKAPYKAVLTHGFVLDEKGFKMSKSLGNVVDPLKVIEGGNNKKQEPAYGADVLRLWVASVDYTGDVCIGNNIIKQTFESYRKLRNTARYLIGNLADYVPEGRDGSTAVPYDDLPSMDKWMLGRLSSVLREVDEAMDDYQFSRATQEILRFASADLSNFYLDVAKDRLYISGVDDFRRRSCQTVLAACLEGFAKAIAPVLPHMAEDIWQNIPYEQQGESTSVFEGGFPAGLREYGEVDAERWDLVRDLRDDVNKALEGARADKLVGASLDAAAYVHCPDEKVREILDGLVGDRDLIHPPVKSNGVDDLRTALMISQVNLVDSAEDVAEKCDDKYVM--KGEKSGCTVGVTKAAGHKCGRCWFYDETVGTTTDDSRRGPDLCQRCDEAID 1008          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: L1JBT3_GUITC (Isoleucine--tRNA ligase n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JBT3_GUITC)

HSP 1 Score: 1260 bits (3261), Expect = 0.000e+0
Identity = 623/975 (63.90%), Postives = 759/975 (77.85%), Query Frame = 2

Query:  341 ESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRVLEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKI--GLEEGDSLKVLKSYTGRDLVGIKYQHPLC-DRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESGEPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREE---LAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPEKHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHARHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGDETLKREPELSNG---VDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWYQCGSVGSHKHHPTLCSRCNGIVE 3238
            E  Y  TV LP T F+QRAN+V REPE+Q FW+E RIY+++ E N G K+VLHDGPPYANG+LHIGHALNKILKDFINRY+++ G KV FVPGWDCHGLPIELKVLQS+K +E+  MTP+ LRKKAAEFA+ TV +Q ESF+RYGVW D+  PYLTL P+YEAAQIE FG M   GHI+RGRKPVNWSPSSRTALAEAELEYP GH S S+YAAF V E + A+         L VA+WTTTPWT+PANLAVAV+E +EYS+VEH  +   K +VA DL G ++AK+  G E+   LK +   TG++L G KYQHPL  +R++ VV+GGDYITTE+GTGLVHTAPGHG EDY TGLKYGL LLSPVDDAG+FTAEAG   +G  VLK+GNE VL  +EEAG L+ +E Y HKYPYDWRTKKPTIFRATDQWFASV+ FR+ A+ AI+ VKWIPEVG+ RI++MVEGR+DWCISRQRSWG+PIPVFYH E+GEP+LT ESI+HIR I+AEHGSDAWWE + A+LLPPS ++EAD+W RG DTMDVWFDSGSSW GV K+  E   L +PAD+YLEGSDQHRGWFQSSLLTSVAA G APYKTVLTHGFVLDEKG+KMSKSLGN +DP  VI GG N+K +P +GAD LRLWV+SVDY+GDV VG +I+KQ+S++YRK+RNTLRYL G+L  FDP+KH+V YE+LPS+DK+MLG L+ V EEV  AYD +QFY+A QA+ +    +LSNFYLD AKDRLYIS+ DD RRRSCQTVLH  L  L+  ++P+LPHMAED WQ LP++      S+F+ GW    +  HE ++W  +R+LR DVN+ +E ARA K++G++LEG V IY  DE  K  + S+L + ++    E S G   VDDLRF+ +VS + +VD+ E V S C E  T++ +DS SGCFVGV+ A G KCERCW+   +VG+H+    +C RC   V+
Sbjct:   19 EGIYSSTVNLPVTNFQQRANSVVREPEIQKFWEENRIYEKLYESNPGTKFVLHDGPPYANGNLHIGHALNKILKDFINRYKVISGHKVKFVPGWDCHGLPIELKVLQSLKKEEKENMTPISLRKKAAEFAKQTVDQQRESFKRYGVWADWSEPYLTLDPKYEAAQIETFGAMLKGGHIYRGRKPVNWSPSSRTALAEAELEYPEGHKSRSMYAAFTVVEPSEAVKPH---SENLKVAIWTTTPWTIPANLAVAVNEKLEYSIVEHHGV---KYVVAKDLKGALAAKLKKGEEDEVELKEIAVITGKELAGTKYQHPLYKERINPVVIGGDYITTESGTGLVHTAPGHGVEDYQTGLKYGLELLSPVDDAGRFTAEAGPLLEGKDVLKDGNERVLELMEEAGALIKEEEYGHKYPYDWRTKKPTIFRATDQWFASVENFREKAMGAIDTVKWIPEVGKNRISAMVEGRSDWCISRQRSWGVPIPVFYHKETGEPLLTAESIDHIRDIIAEHGSDAWWEKDVAELLPPSHKQEADKWERGRDTMDVWFDSGSSWNGVVKSWGEGKALDFPADMYLEGSDQHRGWFQSSLLTSVAAQGTAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPALVINGGKNQKTEPAFGADTLRLWVASVDYTGDVRVGGNIMKQISDSYRKIRNTLRYLLGNLHGFDPKKHSVKYEDLPSVDKWMLGRLARVQEEVRDAYDTFQFYRAYQAILQVCITELSNFYLDIAKDRLYISAEDDVRRRSCQTVLHAALEMLTTAISPMLPHMAEDAWQNLPWEHVS---SVFEHGWKQLDYPSHEGERWDYIRSLRDDVNQCMEAARADKLIGATLEGSVAIYVADEEKKQLVTSLLHEVSMLTS-ETSPGFNKVDDLRFLFLVSDVKIVDTPEDVTSLCNEKHTLSISDSASGCFVGVARAQGKKCERCWFYSDTVGTHEGLENICPRCAQAVK 983          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A6T8JN82_HEMAN (Isoleucine--tRNA ligase n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A6T8JN82_HEMAN)

HSP 1 Score: 1252 bits (3240), Expect = 0.000e+0
Identity = 624/978 (63.80%), Postives = 764/978 (78.12%), Query Frame = 2

Query:  335 KPESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRVLEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGG--LAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDS---------LKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESGEPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAR-EELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPEKHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHARHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGD-ESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAAND--SESGCFVGVSLAPGNKCERCWYQCGSVGSHKHHPTLCSRC 3223
            K +S Y +TV LP+T F QRANAV REPELQAFW +E++Y++  EDN G K+VLHDGPPYANGDLHIGHALNKILKDFINRYQ+LKG KV FVPGWDCHGLPIELKVLQSMKS E+  +TPV+LRKKAA FAR TV KQ +SF+RYGVWGD+ERPYLTL PEYEAAQI+VFG+M   GHI+RGRKPVNWSPSSRTALAEAELEYP GH S S++AAF VT  + AL E     GG  + VAVWTTTPWTLPANLAVAV+E ++Y++V+  S      +VA DLV  +S K+  E  +          L+ +  + G++L G  Y+HPL +R S+VV+GG+YITTE+GTGLVHTAPGHG EDY TGLKY L LLSPVDD G FT EAGE  KG SVL +GN+ V+  + EAG L+ +E Y HKYPYDWRTKKPTIFRATDQWFASVD FRD A+  I KV++IPE G+ RI SMVEGR+DWCISRQR+WG+PIPVFYH E+GEP+LT+ESI+H+R IV + G+DAW+EM+ ADLLPPS ++EADQW +G DTMDVWFDSGSSWAGV + R E+LAYPAD+YLEGSDQHRGWFQSSLLTSVAA G APYKTVLTHGFVLDEKG+KMSKSLGN +DPK++IEGG NKK +PPYGADVLRLWVSSVDY+GDV VG +I+KQ+S++YRK+RNTLRYL G+L+DFDP KH V Y+E+PS+DK+MLG L+ V  EV+ A++ +QFY+ASQ + +    DLSN YLD AKDRLYISS DD  RRSCQTVL   L  LS  +AP++PHMAED WQ +P+  +  ++S+FQ GW T  +A H+ DKW  +R +R DVN A+E AR   ++G+++E +V+I+CGD + +KA ++  +  +++   P      DD+RF+L+ S++ LVDSA+AV  AC E  TV   +  +ESG  VGV+ A G KCERCW+   SVG H+    +C RC
Sbjct:   96 KEDSKYSKTVNLPETNFNQRANAVVREPELQAFWKDEKVYEKAYEDNTGSKFVLHDGPPYANGDLHIGHALNKILKDFINRYQILKGSKVKFVPGWDCHGLPIELKVLQSMKSAEKRELTPVKLRKKAASFARETVDKQMDSFKRYGVWGDWERPYLTLLPEYEAAQIKVFGEMVEGGHIYRGRKPVNWSPSSRTALAEAELEYPEGHVSRSLWAAFPVTMMSEALKE---KAGGEDVRVAVWTTTPWTLPANLAVAVNERLDYALVKAPS--GVCYIVAKDLVDNLSKKMQGEAEEEXXXXXXXXVLEEIAVFKGKELEGSIYKHPLYERESKVVIGGEYITTESGTGLVHTAPGHGVEDYQTGLKYNLDLLSPVDDGGLFTEEAGEDLKGKSVLGDGNQAVIDMMREAGALIKEEPYGHKYPYDWRTKKPTIFRATDQWFASVDSFRDQAMEEIAKVEFIPEKGRSRIESMVEGRSDWCISRQRNWGVPIPVFYHKETGEPLLTKESIDHVRKIVLDKGTDAWFEMDEADLLPPSHKDEADQWVKGKDTMDVWFDSGSSWAGVVQERGEDLAYPADMYLEGSDQHRGWFQSSLLTSVAAQGQAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPKQIIEGGKNKKLEPPYGADVLRLWVSSVDYTGDVRVGSNIMKQISDSYRKIRNTLRYLLGNLYDFDPAKHAVSYDEMPSVDKWMLGKLAEVQSEVKEAFEKHQFYRASQCITQLVIGDLSNLYLDVAKDRLYISSPDDLSRRSCQTVLKVALEMLSTSIAPLMPHMAEDAWQNIPWDVD--HKSVFQFGWPTLDYAPHDADKWATIREVRDDVNSAMELARRGGVVGANMEAKVVIHCGDNDELKALIKGSMQQDSILAYPSPVTVADDIRFILLASQVKLVDSADAVTDACGEEYTVGVKEGQTESGIVVGVAKADGKKCERCWFYDESVGEHEGQDGVCPRC 1066          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: B7FRF4_PHATC (Isoleucine--tRNA ligase n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FRF4_PHATC)

HSP 1 Score: 1238 bits (3204), Expect = 0.000e+0
Identity = 612/980 (62.45%), Postives = 755/980 (77.04%), Query Frame = 2

Query:  329 AKKPESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRV---LEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESG-EPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPEKHT----VPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATG--RHARHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWYQCGSVGSHK-HHPTLCSRCNGIV 3235
            AKKP++ Y  T++LP+T F QRANAV REPELQA W E  +Y ++    ++   E++VLHDGPPYANGDLH GHALNKILKDFINR Q+L G++V ++PGWDCHGLPIELKVLQ+MKSKER  +TPV LR+KAAEFA+ TV KQS +F+RYG++GDFE+PYLTLQPE+EAAQI VFG+MF +G+IFRGRKPV+WSPSSRTALAEAELEYP GH S S+Y AF V   +  LA F  ++  L VA+WTTTPWT+PAN+AVAV+  + YSVV+H   G  KLLVATDL  +++ K  L E ++L +L ++ G+DLVG  Y+HP+ DR S V+ GGDYITTE+GTGLVHTAPGHGQEDY+TGLK GL +LSPVDD GKFT EAGER+ G+ VL EGN  ++ AL+EAG LL  E Y HKYPYDWRTKKPTIFRATDQWFASV  FRD AL A+EKVKW+PEVG+ RI S V+GR DWCISRQRSWG+PIPVFY   +G E +L ++++ H++ + AEHGSD WW+M+ ++LLP   R EAD+W++GTDTMDVWFDSGSSWAGVA++R+ELAYPAD+YLEGSDQHRGWFQSSLLTSVA N  APYKTVLTHGFVLDEKG+KMSKSLGN +DP +VIEGG NKKQ P YGADVLRLWV++ DYSGDV +GD+IIKQ  ++YRKLRNT RYL G+L DF P   T    V Y++LPS+DK+MLG LS+VL  V  A D +Q+ +A Q L RFA+ADLSNFYLD AKDRLYIS+ DD RRRSCQTVL+  L   +K ++PILPHMAED+WQ LP++      S+F+ GW T    +A  +   W  +R +R DVNK LE AR+ K++G+SL+    IY  D   KA L+ ++GD +L   P  +NGVD+LR  LM+S+I+LV++   V  AC E   V++ D+ SGC VGV  A G KC RCW+    VG+H   +  +C RCN  +
Sbjct:   54 AKKPKNAYAPTIVLPETAFSQRANAVIREPELQALWKETNLYHKLSSQAKEAGAERFVLHDGPPYANGDLHCGHALNKILKDFINRKQILNGKQVHYIPGWDCHGLPIELKVLQTMKSKEREALTPVTLREKAAEFAKETVEKQSVAFQRYGIFGDFEKPYLTLQPEFEAAQIRVFGEMFQKGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSIYVAFNVDTPSEVLASFHSTEEPLKVAIWTTTPWTMPANMAVAVNPELSYSVVQHEKTG--KLLVATDLASSLARKFDLPEDETLDILATFPGKDLVGTIYKHPMYDRKSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLEILSPVDDVGKFTIEAGERYVGMRVLAEGNLAMIDALQEAGSLLKAEDYGHKYPYDWRTKKPTIFRATDQWFASVAGFRDQALDAVEKVKWVPEVGKNRIRSFVDGRGDWCISRQRSWGVPIPVFYDKATGKEVLLNKDTLAHVQTLFAEHGSDCWWKMDESELLPEKFRAEADKWQKGTDTMDVWFDSGSSWAGVAQSRDELAYPADMYLEGSDQHRGWFQSSLLTSVANNNIAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPLKVIEGGNNKKQDPAYGADVLRLWVANSDYSGDVLIGDNIIKQTFDSYRKLRNTARYLIGNLADFVPSDSTDSNAVAYDDLPSMDKWMLGRLSAVLRTVNEAMDDFQYQRAIQELLRFASADLSNFYLDVAKDRLYISAMDDARRRSCQTVLYACLEGFTKAISPILPHMAEDIWQNLPYQKSTD--SVFEGGWPTNLMSYAEFDSQTWDLVRLVRDDVNKMLESARSDKLVGASLDAAAYIYVPDSEKKAILEKLVGDASLVAPPVKTNGVDELRTALMLSQIHLVENENDVREAC-EDKYVSSRDTSSGCIVGVGRAVGTKCARCWFYDEEVGNHSLTYADVCQRCNEAI 1028          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A7S4ERA1_9STRA (Isoleucine--tRNA ligase n=2 Tax=Pseudo-nitzschia TaxID=41953 RepID=A0A7S4ERA1_9STRA)

HSP 1 Score: 1236 bits (3198), Expect = 0.000e+0
Identity = 609/978 (62.27%), Postives = 755/978 (77.20%), Query Frame = 2

Query:  338 PESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRVLE---DNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESG-EPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDF----DPEKHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPF-KPEGGYRSIFQAGWAT--GRHARHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWYQCGSVGSHK-HHPTLCSRCNGIV 3235
            P++ +  T++LPQT F QRANA+KREPE+Q FW E  +Y ++ E   +   +++VLHDGPPYANG LH GHALNK+LKDFINR Q L G++V ++PGWDCHGLPIELKVLQ+MKSKER G+TPV LR+KAA FA++TV +QS+SF+RYG++GDFE+PYLTLQP++EAAQI VFG+MF +G IFRGRKPV+WSPSSRTALAEAELEYP  H S S+Y  FEV E +  L EF   +  + VA+WTTTPWT+PANLAVA++  + YSVV HAS G  KL+VATDLV T+++K+GL EG+S +++ +  G DLVG  Y HPL DR S V+ GGDYITTE+GTGLVHTAPGHGQEDY+TGLK GL LLSPVDD+G+FT EAGE FKGL+VLKEGN+ ++ AL   G LL  E Y HKYPYDWRTKKPTIFRATDQWFASV+ FRD+AL A++ V+W+P  G+ RI S V GR DWCISRQRSWG+PIPVFY  E+G E +L + +++H+  I  E GSDAWW+++  DLLP  L+ EAD+W++GTDTMDVWFDSGSSWAGVA  R+ELAYPAD+YLEGSDQHRGWFQSSLLTSVA N  APYKTVLTHGFVLDEKG+KMSKSLGN +DP  VIEGG NKK +P YGADVLRLWV+SVDYSGDV +G +IIKQ  E+YRKLRNT RYL G+L DF     P+   VPY+ELPSLDK+MLG LS  L EV+ A D ++F +A+Q L RFATA++SNFYLD AKDRLYIS+SDDFRRRSCQTVLH ++   +K +A ILPHMAED+WQ LP+ KP     S+F+ GW +   +  +HE +KW  +R++R DVNK LE AR  K++G+SL+    +Y  DE  +  L+S++GD +L      +NGVD+LR  LM+S+I++VDS E +  +C E   ++  D+ SGC VGV+ A G KC RCW+    +G     H   C RCN  +
Sbjct:   55 PKNPFASTIMLPQTGFSQRANAIKREPEIQEFWKEYGLYDKLSEAASEASKDRFVLHDGPPYANGSLHCGHALNKVLKDFINRKQALNGKQVHYIPGWDCHGLPIELKVLQTMKSKERQGLTPVTLREKAATFAKATVEEQSKSFQRYGIFGDFEKPYLTLQPKFEAAQIRVFGEMFKKGFIFRGRKPVHWSPSSRTALAEAELEYPPNHVSKSIYVGFEVVEPSDGLKEFHSKEEPVKVAIWTTTPWTMPANLAVAINPDLSYSVVNHASTG--KLVVATDLVETIASKVGLPEGESFEIVATLPGSDLVGTMYTHPLYDRNSPVLEGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLELLSPVDDSGRFTIEAGEEFKGLNVLKEGNQAMIDALTAKGALLKVEDYGHKYPYDWRTKKPTIFRATDQWFASVEGFRDSALEAVDTVQWVPATGKNRINSFVVGRGDWCISRQRSWGVPIPVFYDRETGNEVLLNDSTLDHVEKIFVEQGSDAWWKLDEVDLLPEELKAEADKWKKGTDTMDVWFDSGSSWAGVASERDELAYPADMYLEGSDQHRGWFQSSLLTSVAVNDKAPYKTVLTHGFVLDEKGFKMSKSLGNVIDPLYVIEGGNNKKLEPAYGADVLRLWVASVDYSGDVGIGPNIIKQTFESYRKLRNTARYLIGNLADFVPAGQPDSTAVPYDELPSLDKWMLGRLSDTLNEVDEAMDVFEFQRATQELLRFATAEMSNFYLDVAKDRLYISASDDFRRRSCQTVLHALIEGFAKAIASILPHMAEDIWQNLPYQKPT---ESVFEGGWPSHLAQFEQHETEKWDLVRSVRDDVNKMLEGARTDKLVGASLDAAAYLYVADEEKRKILESLVGDTSLIVPSVKTNGVDELRTTLMLSQIHIVDSPEKIAESCDEKY-ISTADTSSGCIVGVAKAEGTKCGRCWFYDTQIGKQGLPHDDACQRCNEAI 1026          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A1Z5JP77_FISSO (Isoleucine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JP77_FISSO)

HSP 1 Score: 1225 bits (3169), Expect = 0.000e+0
Identity = 606/977 (62.03%), Postives = 746/977 (76.36%), Query Frame = 2

Query:  332 KKPESYYKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRVLEDNVGE-KYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESG-EPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPE----KHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHARHED--DKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWYQCGSVGSHK-HHPTLCSRCNGIV 3235
            KK ++ +  T+ILP T F QRANAV REPELQA+W E  +Y ++ + N    ++VLHDGPPYANGDLH GHALNKILKDF+NR Q+L G++V ++PGWDCHGLPIELKVLQ+M SKER+G+TPV LR+KAA FA+ TV KQS +F+R+G++GDF++PYLTLQPE+EAAQI VFG+MF +G+IFRGRKPV+WSPSSRTALAEAELEYP GH S S+Y AF V + + AL E   +   L VA+WTTTPWT+PANLAVAV+  + YSVV H   G  KLLVATDL  T++ K  L EG+   V  ++ G DLVG  YQHPL +R S V+ GGDYITTE+GTGLVHTAPGHGQEDY+TGLK GL +LSPVDD GKFTA+AGE+F GLSVL EGN+ ++ AL E G LL  E Y HKYPYDWRTKKPTIFRATDQWFASV  FRD+AL AI+ VKW+P+VG+ RIT+ V GR DWCISRQRSWG+PIPVFY   +G E +L E++++HI+ +  +HGSD WW M+  DLLP S + EA++W +GTDTMDVWFDSGSSWAGVA++R ELAYPAD+YLEGSDQHRGWFQSSLLTSVA N  APYKTVLTHGFVLDEKG+KMSKSLGN +DP +VI+GG N+K +P YGADVLRLWV++ DYS DV +G +IIKQ  E+YRKLRNT RY+ G+L DF PE     + VPY++LPS+DK+MLG L++VL+ V+ A + YQF +A Q + RFATADLSNFYLD AKDRLYIS  DDFRRRSCQTV+   L   +K M+P+LPHMAED+WQ LP+  E  + S+F+ GW     A  E   D+W  +R LR DVNK LE AR  K++G+SL+G   +Y  D+  +A L+ ++GDE L   P  +NGVD+LR  LM+S+++LV S  A+  AC E   ++  D+ SGC VGV  A G KC RCW+    +G H   H   C RCN  +
Sbjct:   45 KKEKNVFASTIILPDTPFSQRANAVVREPELQAYWKESGLYHKLSKQNAASGRFVLHDGPPYANGDLHCGHALNKILKDFVNRKQLLNGKQVHYIPGWDCHGLPIELKVLQTMSSKERSGLTPVTLRQKAAAFAKETVEKQSVAFQRFGIYGDFDKPYLTLQPEFEAAQIRVFGEMFKKGYIFRGRKPVHWSPSSRTALAEAELEYPDGHVSKSIYVAFNVDKPSQALVEHHSAQDPLKVAIWTTTPWTMPANLAVAVNSDLSYSVVFHEKTG--KLLVATDLAETLAKKFALPEGEVFDVRGTFQGSDLVGTTYQHPLYERRSPVLAGGDYITTESGTGLVHTAPGHGQEDYLTGLKNGLEILSPVDDVGKFTADAGEKFVGLSVLAEGNQAIIDALAETGALLKAEDYGHKYPYDWRTKKPTIFRATDQWFASVQGFRDSALKAIDTVKWVPDVGKNRITAFVSGRGDWCISRQRSWGVPIPVFYDRATGKEVLLNEDTLQHIQELFVKHGSDCWWTMDEKDLLPESYKHEAEKWVKGTDTMDVWFDSGSSWAGVAQSRNELAYPADMYLEGSDQHRGWFQSSLLTSVANNDQAPYKTVLTHGFVLDEKGFKMSKSLGNVVDPMQVIKGGNNQKLEPAYGADVLRLWVANCDYSSDVSIGPNIIKQTFESYRKLRNTARYMIGNLADFVPEGQPGSNAVPYDDLPSMDKWMLGRLTAVLKLVDEAMNEYQFQRAVQEILRFATADLSNFYLDVAKDRLYISGIDDFRRRSCQTVIQKCLEGFAKAMSPLLPHMAEDIWQNLPY--ETSHDSVFEGGWPADLAAYPEFAVDEWDLVRELRDDVNKVLEAARNDKLVGASLDGAAFVYVADDRKRAILEKLVGDENLISPPVKTNGVDELRTALMLSQVSLVGSETALKEACEEKY-ISGKDTASGCIVGVRKASGEKCGRCWFYDEQIGKHNLPHGDACQRCNEAI 1016          

BLAST of mRNA_P-fluviatile_contig13.1723.1 vs. uniprot
Match: A0A7S0LI09_9EUKA (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0LI09_9EUKA)

HSP 1 Score: 1224 bits (3166), Expect = 0.000e+0
Identity = 597/968 (61.67%), Postives = 737/968 (76.14%), Query Frame = 2

Query:  350 YKQTVILPQTRFEQRANAVKREPELQAFWDEERIYQRVLEDNVGEKYVLHDGPPYANGDLHIGHALNKILKDFINRYQMLKGRKVSFVPGWDCHGLPIELKVLQSMKSKERAGMTPVELRKKAAEFARSTVSKQSESFRRYGVWGDFERPYLTLQPEYEAAQIEVFGKMFTQGHIFRGRKPVNWSPSSRTALAEAELEYPAGHTSTSVYAAFEVTEATPALAEFMCSDGGLAVAVWTTTPWTLPANLAVAVSETIEYSVVEHASLGARKLLVATDLVGTVSAKIGLEEGDSLKVLKSYTGRDLVGIKYQHPLCDRVSEVVVGGDYITTEAGTGLVHTAPGHGQEDYVTGLKYGLPLLSPVDDAGKFTAEAGERFKGLSVLKEGNEEVLVALEEAGGLLAKEAYQHKYPYDWRTKKPTIFRATDQWFASVDRFRDNALSAIEKVKWIPEVGQRRITSMVEGRNDWCISRQRSWGLPIPVFYHVESGEPMLTEESIEHIRGIVAEHGSDAWWEMETADLLPPSLREEADQWRRGTDTMDVWFDSGSSWAGVAKAREELAYPADLYLEGSDQHRGWFQSSLLTSVAANGFAPYKTVLTHGFVLDEKGYKMSKSLGNTLDPKEVIEGGTNKKQKPPYGADVLRLWVSSVDYSGDVCVGDHIIKQVSEAYRKLRNTLRYLSGSLFDFDPEKHTVPYEELPSLDKYMLGILSSVLEEVEGAYDGYQFYKASQALQRFATADLSNFYLDGAKDRLYISSSDDFRRRSCQTVLHTMLMSLSKVMAPILPHMAEDVWQTLPFKPEGGYRSIFQAGWATGRHARHEDDKWGRLRTLRIDVNKALEQARAAKMLGSSLEGQVLIYCGDESMKAELQSMLGDETLKREPELSNGVDDLRFVLMVSKINLVDSAEAVDSACAEGMTVAANDSESGCFVGVSLAPGNKCERCWYQCGSVGSHKHHPTLCSRCNGIVEGLGLP 3253
            Y+ +V LPQT F QRAN+  RE ELQ FW+E+ +Y+R+ + N GEK+VLHDGPPYANGDLHIGHALNKILKD INRYQML+G++V +VPGWDCHGLPIELKVLQ MKSKER  +TP+ LR+KAA FA+ TVSKQ ESF+RYGVWG+++ PYLTLQPEYEAAQI VFGKM  +GHI+RGRKPV+WSPSSRTALAEAELEYP  H STS+Y A +VTE + ALA +      +AVA+WTTTPWT+PANLAVAV+  ++YSVV HASL   KL+VA+DLV +V+ ++ L EG+ L  +   +G DL    Y+HPL +R S+VV+GGDYITTE+GTGLVHTAPGHGQEDY+TG KYGLPLLSPVDDAGKFT EAGE F GLSVL +GN  V+ AL+EAG LLA E Y HKYPYDWRTKKPTIFRAT QWFASVD FRD+ALSAIE V+W+P +GQ RITSM + R+DWCISRQR+WG+PIPVFY  ++ E +L + +++H++ + AEHGSD WW+ +   LLP  L+ EA +WR+GTDTMDVWFDSGSSW+GVA +R +L+YPADLYLEGSDQHRGWFQSSLLTSVAANG APYKTVLTHGFVLDEKGYKMSKSLGN +DP+ VIEGG NKK +P YGADVLRLWV+SV+Y+ DVC+G  IIKQ  E+YRKLRNT R+L G+L DF P  HTVPY+ELP  D+YMLG+L+ V+ E + A+D YQF +A  A+Q+F  ADLSNFYLD  KDRLYIS+  + RRRSCQTVL     +L+  +APILPH+AED+W  LP+KP     S+F+  W    +   +   W  +R LR   N+ +E AR  K LG+SLE ++L++  D  +K  L+   G      E    NGVD+LRF  + S++ L+++AEAV       +     +      VGV+ A G+KCERCW+    + S   +P +C RC+  +  +  P
Sbjct:   47 YRSSVSLPQTSFSQRANSATRELELQRFWEEQGVYERLRDSNPGEKFVLHDGPPYANGDLHIGHALNKILKDIINRYQMLQGKRVCYVPGWDCHGLPIELKVLQGMKSKERQALTPISLREKAAAFAQETVSKQRESFKRYGVWGEWDSPYLTLQPEYEAAQIGVFGKMALKGHIYRGRKPVHWSPSSRTALAEAELEYPDKHVSTSIYVALKVTEPSEALAPYTTDGTPVAVAIWTTTPWTIPANLAVAVNADLKYSVVSHASLDF-KLVVASDLVSSVAGRLSLPEGEELSTIAVLSGADLEKSVYKHPLYERTSQVVIGGDYITTESGTGLVHTAPGHGQEDYITGQKYGLPLLSPVDDAGKFTTEAGEPFVGLSVLGDGNTAVITALQEAGALLASEGYAHKYPYDWRTKKPTIFRATSQWFASVDAFRDDALSAIESVRWLPAIGQNRITSMTQSRSDWCISRQRTWGVPIPVFYDRDTDEVLLNKATLDHVQALFAEHGSDVWWQQDEEFLLPDELKSEAARWRKGTDTMDVWFDSGSSWSGVADSRSKLSYPADLYLEGSDQHRGWFQSSLLTSVAANGVAPYKTVLTHGFVLDEKGYKMSKSLGNVIDPRSVIEGGNNKKTQPGYGADVLRLWVASVNYAADVCIGPSIIKQTFESYRKLRNTARFLLGNLHDFVPASHTVPYDELPETDRYMLGLLAEVMAEAKAAFDDYQFSRALGAIQQFVVADLSNFYLDVGKDRLYISAPGEARRRSCQTVLSITTHALAAALAPILPHLAEDIWLNLPYKPPT--TSVFENPWPQLDYQVSDAAAWSAVRELRDVANRCIELARIDKALGASLEAKLLLHVEDPELKEALRRWQG------EGAAGNGVDELRFFFLTSQVELLETAEAVAQTSLHSIV---REEAPAATVGVAKADGHKCERCWHYHVDLASSVSYPNVCPRCSESLSLMAFP 1002