mRNA_P-fluviatile_contig12.1356.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: A0A6H5J7N3_9PHAE (E1 ubiquitin-activating enzyme n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5J7N3_9PHAE)

HSP 1 Score: 1845 bits (4778), Expect = 0.000e+0
Identity = 926/1060 (87.36%), Postives = 984/1060 (92.83%), Query Frame = 2

Query:   80 GGREISLPFSLNL-EPSAKRVRTMPPRPHPAKGGGGEASGIEQNGSGVDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQGMTELEGREFTITEKSPFSFEIDFDTSACGTFVSGYVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTEKGSLPEAGDMDQGEEVYTMAKGFNDEEGGFKAEGLDDCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLSKEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            G REIS P S  + EP++KRVRTMPP   PA+GG    +G   + +GVDESLYSRQLYVMGHEAQRRMATSNVLIVG NGLGAEVAKNVILAGV+SVTLLDDG AEW+DLSAQFYL+EADLG PRAAACV++LAELNRYV VST +G +TEA++ S+Q VVMIDAPL+EQLRVND CHAKGVCFISCDARGVFAYAFCDFG++FVVSD DGNQAASCVV+SVTKD VGLVTVMDDQRHNLVTGDVVTFNS+QGMTELEGREFTITEK PFSFEID DTS+ GTFVSGYVNQVKKPSTLSFLPLREAL+KPEPFMETDFAKIGRPGVLHQAFRGLDK+R +KGSLPEAGDM+Q E V+ +AKG N E+G FKAEGLDD KDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDA EALPEKPL+KEEVQP+GCRYD SIMVFGKTMQ LLGKQKLFLVGAGAIGCEMLKNWAMMG+GC GDGQV VTDMD+IEKSNLSRQFLFRESDIGR KSLTAA AV+AMNPSL IKPYEAKCAQETEELFSDDFYSGL+AVCTALDNVEARLYMDQRCLFY+KPMLESGTLGTKGNTQIVVP+LTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYL DPNYT FLNSQHNTKLETLTRISESL SSRPS+F DCI+WARLQFQ RFHNEIAQLLHNFPVDS+TSSGNPFWSGAKRPP PLDFD +D LH GFVKGAAVLRA  YGIKPPTDD+VY + L +++VP FKP+DGVKIATT+AEAKEQ  G  XX  QLEDVDAQCERML ELPK  DMK+FRLEVVEFDKDLD+HMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLV MEVYKIMQ+KPLESYKNWFLNLALPQFSCSEPLPPAKT T+IKGSEWKWSAWDSLELEGAD TLQQLFDIMK+KYGLEVTMLSHGVSILYSFFA+KKKIAERLPM+LPKIVELVTKK IPA QRYLIFEVCVSD++TDDEREVPYIRLKLR
Sbjct:   46 GSREISSPQSYTVAEPASKRVRTMPP--DPAEGGKAVEAGGGGSKAGVDESLYSRQLYVMGHEAQRRMATSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLSEADLGNPRAAACVSRLAELNRYVGVSTTTGEVTEAMIGSYQVVVMIDAPLDEQLRVNDICHAKGVCFISCDARGVFAYAFCDFGESFVVSDTDGNQAASCVVSSVTKDAVGLVTVMDDQRHNLVTGDVVTFNSIQGMTELEGREFTITEKGPFSFEIDCDTSSLGTFVSGYVNQVKKPSTLSFLPLREALSKPEPFMETDFAKIGRPGVLHQAFRGLDKYRADKGSLPEAGDMEQAEAVFELAKGCN-EDGDFKAEGLDDSKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAFEALPEKPLAKEEVQPLGCRYDGSIMVFGKTMQALLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDGDGQVHVTDMDNIEKSNLSRQFLFRESDIGRAKSLTAARAVRAMNPSLNIKPYEAKCAQETEELFSDDFYSGLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLQDPNYTTFLNSQHNTKLETLTRISESLDSSRPSSFGDCIKWARLQFQARFHNEIAQLLHNFPVDSVTSSGNPFWSGAKRPPCPLDFDPNDALHMGFVKGAAVLRARMYGIKPPTDDAVYASTLSQMEVPVFKPVDGVKIATTEAEAKEQATGXXXXXAQLEDVDAQCERMLGELPKPADMKDFRLEVVEFDKDLDEHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVSMEVYKIMQEKPLESYKNWFLNLALPQFSCSEPLPPAKTATMIKGSEWKWSAWDSLELEGADITLQQLFDIMKEKYGLEVTMLSHGVSILYSFFASKKKIAERLPMTLPKIVELVTKKGIPASQRYLIFEVCVSDMETDDEREVPYIRLKLR 1102          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: A0A836CIM1_9STRA (Ubiquitin-activating enzyme E1 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CIM1_9STRA)

HSP 1 Score: 1359 bits (3517), Expect = 0.000e+0
Identity = 682/1026 (66.47%), Postives = 801/1026 (78.07%), Query Frame = 2

Query:  218 VDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQGMTELEGREFTITEKSPFSFEIDFDTSACGTFVSGYVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTEKG-SLPEAGDMDQGEEVYTMAKGFND-EEGGFKAEGLD--DCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKP----LSKEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQ------LEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKL 3253
            VDESLYSRQLYVMG EAQRRMATS+VLIVGVNGLG EVAKNVILAGV+SVTLLD   A W+DL+AQFYLTEAD+GKPRAAACV KLAELNRYVS       LT  LVS FQ VV+ID PL  QL V+DFCH+KG+C I+ D RGVFA  FCDFG AFVVSD +  QA SC+V+ VTKD   LVTVMDDQRH L TGDVVTF SV+GM ELEGREFTITE+ P++FEI+ DT+A GTFVSGYVNQVK+P T++F PLR AL +PEPFMETDFAK GRPGVLHQ FR L ++R E G +LP  G+  Q + +  +A+  N     G+KA+GLD  +   V+  L+  +RGV++PVCA +GG+VGQEVLKA SGKF PIRQW+Y+DA EALP       L+ EEVQP  CRYD  IMVFGKT+Q  LG Q LFLVGAGAIGCEMLKNWAMMG+GC   G + VTDMDHIE+SNLSRQFLFRE  IG+PKS TAA A +AMN  +K+  +E KCA +TE +F+DDFY+ LT VCTALDNVEARLYMDQ+CLFY+KPM ESGTLGTKGNTQ+VVP+LTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQW+RDWFEG F Q+A D N YL+ P++ A L +Q N +LETL R+ E+L S RP  +  C++WARL+F+  FHN +AQLLHNFP DS+TSSG PFWSGAKRPP+P+ FD  DPLH  FV+ AA LRA NYGI    DD+     L  + VP+F P +GVKIA T+AEAKE      XX         + D DAQC  ++  LP+   +  FRL  VEFDKD+D HM  VTAASNLRAR YKIPEADMHRSR IAGKIIPAIATTTALVTGLVC+E+ K+ Q+KPLE+YKNWFLNLALPQF+CSEP+PPA T   +KG EW+WSAWD ++++  D TL  LF +++D+YGLEVTMLSHGVSIL+SFFA+KKK AERLPM + ++V  VTK+E+   QRYLIFEVC SDV T ++ E+PY+R KL
Sbjct:    3 VDESLYSRQLYVMGREAQRRMATSDVLIVGVNGLGVEVAKNVILAGVKSVTLLDRTPASWSDLAAQFYLTEADIGKPRAAACVNKLAELNRYVSC--VEEDLTTTLVSRFQVVVLIDVPLSVQLEVDDFCHSKGICCIAADVRGVFASVFCDFGPAFVVSDKNDTQAVSCLVSGVTKDSPALVTVMDDQRHQLETGDVVTFASVEGMPELEGREFTITERGPYAFEINCDTTAFGTFVSGYVNQVKQPVTVAFKPLRAALDQPEPFMETDFAKFGRPGVLHQGFRALHRYRAEHGGALPAPGNSAQADALLALARELNSGSSDGYKADGLDGAEAARVLRALASTSRGVVSPVCAAVGGVVGQEVLKAASGKFMPIRQWLYWDAEEALPAAAEGGVLAVEEVQPQNCRYDGQIMVFGKTLQAALGAQNLFLVGAGAIGCEMLKNWAMMGVGCGPGGAIHVTDMDHIERSNLSRQFLFREEHIGKPKSATAAAAARAMNGDMKLSAHELKCAPDTEGVFNDDFYAHLTGVCTALDNVEARLYMDQKCLFYKKPMFESGTLGTKGNTQVVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWARDWFEGAFSQSAGDANAYLEMPDFLAQLAAQPNARLETLRRVEEALVSGRPERYAQCVQWARLRFEDLFHNTVAQLLHNFPADSMTSSGTPFWSGAKRPPTPVAFDADDPLHVAFVRAAADLRAANYGIAAAADDAAVLAALSSVIVPDFTPAEGVKIAVTEAEAKEXXXXXXXXXXXXXXXXFMMDTDAQCAALVDALPEPASLGAFRLAAVEFDKDVDAHMVLVTAASNLRARCYKIPEADMHRSRLIAGKIIPAIATTTALVTGLVCLELIKVAQEKPLEAYKNWFLNLALPQFACSEPIPPATTAVKLKGREWRWSAWDCIDVDRGDITLAALFALLRDEYGLEVTMLSHGVSILFSFFASKKKAAERLPMRMTQVVAAVTKREVAPTQRYLIFEVCASDVKTSEDVEIPYLRFKL 1026          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: A0A7S2G964_9STRA (E1 ubiquitin-activating enzyme n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2G964_9STRA)

HSP 1 Score: 1170 bits (3027), Expect = 0.000e+0
Identity = 603/1041 (57.93%), Postives = 745/1041 (71.57%), Query Frame = 2

Query:  185 EASGIEQNGSGVDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQGMTELEGREFTITEKSPFSFEIDFDTSACGTF-VSGYVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTEKG-SLPEAGDMDQGEEVYTMAKGFNDE--EGGFKAE----GLDDCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLSKEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGG------QLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGS----EWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKL 3253
            +A  +E+  + +DE LYSRQLYVMG E Q RMA S+VLIVG+NGLG E+AKNVILAGV+SVTL D     W DLSAQFYL EA+LG+PRAA    KLAELN+YV V+     L    +S+FQ VVMI+AP E QL VND+CHA G  FIS +  GVF   FCDFG  FVVSD++G  A+SC+VAS+T+    LVTV+DDQRH L TG VV  + + GM EL GR+FTIT K  +SFEID DT+A   +  +GYVN++K+P+TL+F  LRE+L  P     +DF K  RPG+LH AFR LD ++++ G + P  G+++   E+       N+   EG FK E    G +    V+ +LS+G+ GV+NP+CA +GG+VGQEVLKACSGKF P+ Q+ Y+DA EALP++PL+ ++V P GCRYD+ I+VFG  MQE +     FLVG+GAIGCEMLKNWA+MG+     G V +TDMD IEKSNLSRQFLFR SDIG+ K+  A    + MNP++KI  YEA+   ++E LF+DDF+  L  V  ALDN+EARLYMD RCLFYQKP+LESGTLGTKGNTQ+VVPHLTENYGA+RDPPEKSIPVCTLKNFPNQIEHTLQWSRD FEG FKQ  ED N YL  P++   L  Q +TKL+TL  +  +L   R  +FDDCI W RL F+  ++N+I QLLHNFP D +TS+G PFWSG+KR P  L FD SDPLH  ++K  A LRA NYGIK   DD+ +   L  + VP F P DGVKI T +           XX        +L DVDAQC+ ++ +LP    +  F+L  VEFDKD+D+HMEFVTAASNLRAR YKIPEADMHRSR IAGKIIPAIATTTALVTGLVC+E+YK++Q KP+E+YKN F+NLA+P F+ SEP PPA T   I+      EWKWSAWD L+++  D TL+QL   M+D YGLE+TMLSHGVSILYSFF NKKK   R+PM++ ++V  VT KE+P  Q+YLIFEV  S  D DDE +VP +RL L
Sbjct:    9 DAMDVEEQPTEIDEGLYSRQLYVMGREGQARMAVSDVLIVGMNGLGVEIAKNVILAGVKSVTLHDPTPTSWLDLSAQFYLGEAELGQPRAAVSAPKLAELNQYVKVTCRDDELNPEFLSNFQCVVMINAPYETQLAVNDYCHANGKQFISSEVAGVFGSVFCDFGPEFVVSDINGEAASSCIVASITQASPALVTVLDDQRHGLETGAVVQLDGLDGMEELNGRQFTITFKDAYSFEIDCDTTAMSPYKTAGYVNEIKQPTTLAFKSLRESLTAPGEITLSDFGKWDRPGLLHMAFRALDVWKSKHGGAYPTPGNLEHAGELMGELTTLNEAAGEGEFKLEAEQLGSEATVKVVQQLSVGSAGVINPMCALLGGVVGQEVLKACSGKFMPLTQFFYFDATEALPDQPLTIDQVTPTGCRYDSQIVVFGSEMQEKIMALNYFLVGSGAIGCEMLKNWALMGLAAGESGHVHITDMDSIEKSNLSRQFLFRPSDIGKMKATAATAKAQEMNPAMKITVYEARVGSDSEGLFNDDFFEALDGVTNALDNIEARLYMDSRCLFYQKPLLESGTLGTKGNTQMVVPHLTENYGATRDPPEKSIPVCTLKNFPNQIEHTLQWSRDMFEGMFKQTMEDTNTYLTSPDFAETLRQQQSTKLDTLKNVKAALVDDRALSFDDCIAWTRLNFEVDYNNKIRQLLHNFPEDQVTSTGQPFWSGSKRAPVALVFDASDPLHLAYIKAGANLRASNYGIKGTWDDAYFLAFLPNVVVPGFTPKDGVKIKTPEEXXXXXXXXXXXXXXXXXXDEELMDVDAQCDEIIAQLPTPASLAGFQLNPVEFDKDIDEHMEFVTAASNLRARNYKIPEADMHRSRLIAGKIIPAIATTTALVTGLVCLELYKVIQSKPVEAYKNGFVNLAIPVFAFSEPQPPATTLAKIQKDGAPFEWKWSAWDRLDIKLGDITLKQLIAHMEDAYGLELTMLSHGVSILYSFFQNKKKTKARMPMTMSQLVTEVTGKEVPPEQKYLIFEVMCS-TDEDDEVDVPCVRLFL 1048          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: A0A7S1TWZ4_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1TWZ4_9STRA)

HSP 1 Score: 1149 bits (2972), Expect = 0.000e+0
Identity = 594/1026 (57.89%), Postives = 731/1026 (71.25%), Query Frame = 2

Query:  218 VDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVS-TASGP-LTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQGMTELEGREFTITEKSPFSFEID-FDTSACGTFV-SGYVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTEKG-SLPEAGDMDQGEEVYTMAKGFN--------DEEGGFKAEGLDDCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLSKEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            VDE LYSRQLYVMGHEAQRRMA+S+VLIVG+NGLG EVAKNVILAGV+S+TL D   A + DLSAQFYLTEADLG+PRAAA  AKLAELN YV VS T  G  L  A +S F+ VV ID  L EQLR+ND C   G CF++ D RGV A  FCDFG +F   DVDG  AAS ++++VT++    VT ++D RH L TGD VT   V+GM EL G+EF IT   P++FE+   D +    ++  GYVNQVKKP  LSF  +REAL  P  F+  DFAK+ RP VLHQAFRGL  +  E G +LP  GD    EEV+  A+  N          E     E  +D KDV+LR++L A GV +P+CA MGG++GQEVLKA SGKF PIRQW Y DA E L ++PL  EEV P G RYD  I V+G T+Q  +  Q++FLVGAGAIGCEMLKNWAMMG+GC   G V VTDMD IE+SNLSRQFLFR +DIG+PKS TAA + K MN  +++ PYEAK A  TE++F  DFY GL AVCTALDN++ARLYMD RCLF+  PM ESGT+GTKGNTQIVVPHLTE+YGA+RDPPEKS+PVCTLKNFP+QIEHT+QW+RDWFEG FKQ  + VN YL D  +   L  Q N +LETLT +  SL   RP +FDDC+ WARLQF + F N I QLLHNFP+D +T+SG PFWSG+K+PP+PL F  SD +H  F++  A LRA  YGI P TDD      L  I VP F P DGVKIA  + EA E+ +  S  GG    VD++  R++  LP    +  FRL  V+FDKD+D+ M  V A SNLRAR YKIPEADMH SRQIAGKIIPAIATTTA+VTGL+ +E++K++QKKPLE+YKN F NLA+P F+ SEP PP KT  +IKG E  ++AWD L+++G   TL+ L D M+D+YG+EV MLS+G +I++SF ++ KK  ER+P+ +  ++ELVTK E+P L +Y+       D+DT DE ++P +R +LR
Sbjct:   21 VDEGLYSRQLYVMGHEAQRRMASSDVLIVGLNGLGVEVAKNVILAGVKSLTLYDPTPASYGDLSAQFYLTEADLGQPRAAASKAKLAELNEYVQVSLTPEGQALDGAYLSGFRVVVAIDQLLAEQLRLNDLCRECGACFLAGDVRGVAAAIFCDFGASFTCYDVDGEPAASAMISAVTQENPAAVTCLEDHRHGLETGDFVTITGVEGMEELNGQEFQITVTGPYTFELPTVDATGYANYIRGGYVNQVKKPVELSFRTMREALVSPGEFLLADFAKMDRPPVLHQAFRGLHAYMREHGGALPPPGDAAAAEEVWQEAQRINAAAKTDGDGSEAALHVENFEDAKDVVLRVALVAAGVTSPMCAAMGGVLGQEVLKAASGKFCPIRQWFYIDALETLSDEPLPAEEVAPRGSRYDGQIAVYGATLQTKICAQRVFLVGAGAIGCEMLKNWAMMGVGCGDAGGVHVTDMDRIERSNLSRQFLFRSTDIGQPKSTTAAASAKRMNTDMRVSPYEAKVAPSTEDVFDTDFYDGLDAVCTALDNIDARLYMDSRCLFFGLPMYESGTMGTKGNTQIVVPHLTEHYGATRDPPEKSVPVCTLKNFPHQIEHTIQWARDWFEGAFKQTPDSVNAYLSDAEFLKKLERQPNIRLETLTAVMRSLVGDRPRSFDDCVAWARLQFDKLFRNAILQLLHNFPLDHVTTSGAPFWSGSKKPPTPLVFSTSDEVHLEFIRSVARLRAQIYGITPSTDDESIVAALDGIDVPAFSPADGVKIAADEKEAAEE-EKSSLSGGTS--VDSEVSRIVDALPAPASLAGFRLSPVDFDKDVDEQMAVVMAVSNLRARNYKIPEADMHASRQIAGKIIPAIATTTAMVTGLISLEMFKVLQKKPLEAYKNGFANLAIPVFAFSEPGPPEKTKAMIKGEEKAFTAWDFLQVDGTGMTLKGLIDYMEDEYGVEVQMLSYGQAIVHSFMSSAKKRKERMPLPVKDVIELVTKTELPPL-KYITLAAMCGDLDTGDEVDLPPLRFRLR 1042          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: I2CSG2_NANGC (E1 ubiquitin-activating enzyme n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=I2CSG2_NANGC)

HSP 1 Score: 1132 bits (2927), Expect = 0.000e+0
Identity = 578/1042 (55.47%), Postives = 736/1042 (70.63%), Query Frame = 2

Query:  188 ASGIEQNGSGVDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVST----ASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQGMTELEGREFTITEKSPFSFEIDFDTSACGTFV-SGYVNQVKKPSTLSFLPLREALAKPE--PFMETDFAKIGRPGVLHQAFRGLDKFRTE-KGSLPEAGDMDQGEEVYTMAKGFNDEEGG---FKAEGLD-DCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLSKEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDS----VYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLE---DVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            ++G+E     +DE LYSRQLYVMGHEAQ+RM  S+VLIVGV GLG EVAKNVILAGV+SVTL D     + DL+AQFYL+EADLGKPR  A   +LAELN YV V      A   LT   V  +Q + + + PL EQLR+N   H   + FI+ + RG+    FCDFG+AF+VSD     A + ++ S+T++   +V+V+++ RH L TGDVV    V+GM E+  +E+ IT   P +F+I   T     +V  GY+ Q+K+  TL+F PL +AL       F+  DFAK+ RP +LH  FR L  F  + +G  P  G  +  EEV  +A   N+   G      E L+ D   VI +L+LGA GVLNP+ +  GGIVGQEVLKACSGKFSPI+QW Y+DA E LP +P+S+ E  P+ CRYD  I VFGK++Q++LG+Q  FLVGAGAIGCEMLKNWAMMG+G    G V VTDMD IEKSNLSRQFLFR +DIG+ KS+ A  A + MNP LKI  YE +   +TE++F+D+FY  LT VCTALDNV+ARLYMDQRCLFYQ PMLESGTLGTKGNTQ+VVPHLTENYGA+RDPPEKSIPVCTLKNFPNQIEHT+QW+RDWFEG FKQ  EDVN YL +P Y   L +Q NTKLETL RI  SL + RP++F DC+ WARL+F++ F+N I QLLHNFP+D +TS+G PFWSG K+PP+PL F   DPLH  FV   A LRA  Y +  P  ++         L  + VP+F P DGVKIA TD EAK+Q + G   GG +    D++A+CE ++  LP    +  F L+ ++FDKD+D HM  VTA SNLRA  YKIPEADMH++RQIAGKIIPAIATTTALVTGLVC+E+YK++ KKP+E +K+ F+NLALP F+ SEP PPA  T ++KG EWKWSAWD +++EG D TL++    M++++GLEV+MLSHGVS+L+S   ++K I  R+PM L ++V LVTKK +P  +++LI EV   DV+  D+ EVPY+RL+++
Sbjct:    7 STGMEPGHENIDEGLYSRQLYVMGHEAQKRMQASDVLIVGVAGLGIEVAKNVILAGVKSVTLFDPAPVAYPDLAAQFYLSEADLGKPRDQASAPRLAELNPYVPVHVLEPEAGEALTAEAVKRYQVLCVTNRPLAEQLRLNAITHPLNIAFIASEVRGLCGSVFCDFGEAFLVSDPSDEPAINAMIGSITQEDPAVVSVLEEHRHGLETGDVVMIAEVEGMPEVNNQEYRITVTGPSTFQIPISTKGASPYVRGGYLQQIKQARTLTFQPLEKALGASGLGDFVLADFAKMDRPPLLHLGFRALHAFMAKHQGLAPVPGRREDAEEVLALAHSLNEATEGELQIPREVLEKDGARVIRQLALGAAGVLNPMASFFGGIVGQEVLKACSGKFSPIKQWFYFDALECLPTEPVSEAEAAPLNCRYDGQIAVFGKSVQQILGQQAYFLVGAGAIGCEMLKNWAMMGVGTGEKGAVHVTDMDRIEKSNLSRQFLFRANDIGQAKSVCAVRAAQTMNPDLKICAYENRVGADTEDIFNDEFYEQLTGVCTALDNVDARLYMDQRCLFYQLPMLESGTLGTKGNTQVVVPHLTENYGATRDPPEKSIPVCTLKNFPNQIEHTVQWARDWFEGVFKQAPEDVNAYLSNPEYLPHLQTQQNTKLETLRRIQASL-NDRPTSFQDCLAWARLRFEELFNNTIRQLLHNFPIDQVTSTGTPFWSGPKKPPTPLSFHPDDPLHLAFVSSVAALRASMYALPAPPSEACAPLAVKAALENVMVPDFSPQDGVKIAVTDTEAKQQAEQGGSDGGAVTSSLDLEARCEAIIASLPPPSALTGFTLQPIDFDKDVDAHMAVVTATSNLRAANYKIPEADMHKTRQIAGKIIPAIATTTALVTGLVCLELYKLVLKKPIEDFKSAFVNLALPLFTFSEPQPPASQTAVVKGEEWKWSAWDRIDIEG-DLTLREFLKYMEEQFGLEVSMLSHGVSLLHSSLTSRKNIELRMPMRLSEVVGLVTKKPVPEGKKFLILEVICMDVEGGDDVEVPYVRLRIK 1046          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: A0A6A3N8N5_9STRA (E1 ubiquitin-activating enzyme n=4 Tax=Phytophthora TaxID=4783 RepID=A0A6A3N8N5_9STRA)

HSP 1 Score: 1120 bits (2897), Expect = 0.000e+0
Identity = 578/1040 (55.58%), Postives = 727/1040 (69.90%), Query Frame = 2

Query:  188 ASGIEQNGSG-VDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEL-EGREFTITEKSPFSFEID-FDTSACGTFVSG------YVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTE-KGSLPEAGDMDQGEEVYTMAKGFNDEEGG---FKAEGLD--DCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLSK--EEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            A  I+Q G+  +DE LYSRQLYVMG EAQ RM  SNVLI+G+NGLG E+AKNVILAGV+SVTL DD  A   DL++QFYL+EAD+GK RAA  V KLAELN YV V   SG +TE  +  F+AVV+++ PL+E  R+N  CHAK + FI+ +ARGVF   FCDFGD F+VSD DG +  SC+++S++     L+ V DD RH L TGD+V+F  V G   L +G+   +T   PF+F +D  D +    F  G      YV QVK+P    F  L  ALA P  F+ +DFAKIGR  +LH AF+ LD ++ + +G  P+ G M    EV ++A   ND+      F  E +D  D K VI  L+ GA GVL+P+ A +GGIVGQE LKACSGKF+PI+Q+ Y+DA E LPE   +   +E  PVG RYD  I+VFG+ +QE +    +FLVGAGAIGCEMLKNWAMMG+  + D  + +TDMD IEKSNL+RQFLFR  D+ + KS  AA A+K MNP + ++ Y ++   E+E+ F+DDF+  L+ VCTALDNVEARLYMDQRCLFY+ PM ESGTLGTKGNTQIVVPH TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVN+YL+ P +   LN Q NTK+ETL R+  SL  +RP +F+DCI WAR +F+  F N+I QLLHNFP+D LT+SG PFWSG KRPP+P+ FD  DPLH  F+   A  RA NYG+K  TD   +T VL +I VPEF P  GVKIA +DAE KE   GG+  G  LED DAQCE +L ELPK  D+  +R+E +EFDKD D HME + + SNLRAR YKIPE DMH+SR IAGKIIPAIATTTALVTGLVC E  K+ Q KPL+ YKN F+NLALP F+ +EP+ P  T T++KG E+KW+AWD LE++  D TL++     + +Y  EVTMLS+GV+ILY+ ++ K +  ER+ M +  +V  VTKK I +  +YL+ EVC  D D +D  E+PY+R   +
Sbjct:   66 AMDIDQTGAAPIDEGLYSRQLYVMGREAQLRMGASNVLIIGLNGLGVEIAKNVILAGVKSVTLHDDAPATKLDLASQFYLSEADIGKSRAAVSVKKLAELNPYVPVRCHSGEITEEFLLGFRAVVVVNVPLKEAKRINAVCHAKSIAFIATEARGVFGSVFCDFGDEFIVSDRDGVEPVSCLISSISNSAPPLIAVSDDTRHGLETGDLVSFREVAGFPFLNDGKPREVTVTGPFTFTLDTIDDAGKRLFEEGQPSSGGYVTQVKQPLVAKFKDLESALATPGEFLISDFAKIGRSELLHVAFQALDAYQEKHQGRYPKPGCMRDAGEVLSLAAELNDQSASKKHFSVENIDADDSKKVIQALAAGATGVLSPMAAFLGGIVGQEALKACSGKFTPIQQFFYFDAIECLPETVYADTPDEFAPVGSRYDGQIVVFGRKVQEKIKNLNMFLVGAGAIGCEMLKNWAMMGVASNKDATIHITDMDTIEKSNLNRQFLFRSKDVQQAKSSVAARAIKDMNPDVNVQSYVSRVGAESEDQFNDDFFESLSGVCTALDNVEARLYMDQRCLFYRLPMFESGTLGTKGNTQIVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNRYLEGPAFMKELNEQQNTKVETLERLKSSLVDNRPLSFEDCISWARFKFEYLFSNQIKQLLHNFPLDQLTTSGTPFWSGPKRPPTPIAFDAKDPLHLDFIVSVANSRAKNYGLKGHTDRDTFTQVLAEIHVPEFSPRKGVKIAASDAELKE---GGAAPG--LEDADAQCESILNELPKPSDLAGYRMEPIEFDKDDDSHMEVIVSVSNLRARSYKIPEEDMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKVFQDKPLDHYKNGFVNLALPLFTFAEPIEPKATKTMLKGEEYKWTAWDRLEVDRGDMTLKEFLAYFEKEYDAEVTMLSYGVTILYAMYSQKSRSKERMAMKISDLVRTVTKKPIDSKLKYLVLEVCAMDADGEDV-ELPYLRYHYK 1099          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: H3G877_PHYRM (E1 ubiquitin-activating enzyme n=33 Tax=Phytophthora TaxID=4783 RepID=H3G877_PHYRM)

HSP 1 Score: 1113 bits (2879), Expect = 0.000e+0
Identity = 579/1040 (55.67%), Postives = 725/1040 (69.71%), Query Frame = 2

Query:  197 IEQNGSGVDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQG---MTELEGREFTITEKSPFSFEID---------FDTSACGTFVSGYVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTE-KGSLPEAGDMDQGEEVYTMAKGFNDE---EGGFKAEGLD--DCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLS--KEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            I+Q+GS +DE LYSRQLYVMG EAQ RM  SNVLIVG+NGLG E+AKNVILAGV+SVTL DD  A   DL++QFYLTEAD+GKPRAA  + KLAELN YV V   +G +TE  +  F+AVV+++APL E  R+N  CHAK + FI  +ARGVF   FCDFGD F+VSD DG +  SC+++S++     LVTV DD RH L TGD+V+F  V G   + + + R+ T+T   PF+F +D         FD     +   GYV QVK+P   SF  L  AL  P  F+  DFAKIGR  +LH AF+ LD F+ + +GS P+ G M   EEV  +A   N +   +  F  E +D  D K +I  L+ GA GV+ P+ A +GGIVGQE LKACSGKF+PI+Q+ Y+DA E LP+   +   +E  P G RYD  I+VFG+ +QE +    LFLVGAGAIGCEMLKNWAMMG+  + +G + +TDMD IEKSNL+RQFLFR  D+ + KS  AA A+K MNP + ++ Y ++   E+E+ F+DDF+  L+ VCTALDNVEARLYMDQRCLFY  PM ESGTLGTKGNTQ+VVPH TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVN+YL+ P +   LN Q NTK+ETL R+  SL   RP +F+DCI WAR +F+  F N+I QLL NFPVD LT+SG PFWSG KRPP+P+ FDV DPLH  FV   A  RA NYG+K   D   +  VL  I VPEF P  GVKIA +DAE KE   GG+  G  LEDVDAQCE +L+ELPK   +  +R++ +EFDKD D HME + + SNLRAR YKIPE DMH+SR IAGKIIPAIATTTALVTGLVC E  K+ Q+KPL+ YKN F+NLALP F+ +EP+ P  T T++KG E+KW+AWD LE++  D TL++     + +Y  EV+MLS+GV+ILY+ ++ K +  ER+ M +  +V  VTKK I    +YLI EVC  D D +D  E+PY+R   +
Sbjct:    3 IDQSGS-IDEGLYSRQLYVMGREAQLRMGASNVLIVGLNGLGVEIAKNVILAGVKSVTLHDDTPASTLDLASQFYLTEADVGKPRAAVSLNKLAELNPYVPVRCHTGEITEDFLLGFRAVVLVNAPLTEAKRINAICHAKSIAFIMTEARGVFGSVFCDFGDEFIVSDRDGVEPVSCLISSISNTVPPLVTVNDDARHGLETGDIVSFREVSGFPFLNDSKPRKVTVT--GPFTFTLDTVDDADKERFDQGQPSS--GGYVTQVKQPLVTSFKNLESALVAPGEFLINDFAKIGRSELLHVAFQALDAFQEKHQGSYPKPGSMQDAEEVSALASELNKQAISKKQFSVENVDAADSKKIIQALAAGALGVIAPMAAFLGGIVGQETLKACSGKFTPIQQFFYFDAVECLPDAVYAGTPDEFAPAGSRYDGQIVVFGRQLQEKIKSLNLFLVGAGAIGCEMLKNWAMMGVASNKNGIIHITDMDTIEKSNLNRQFLFRSKDVQQAKSSVAARAIKDMNPDVNVQAYVSRVGAESEDQFNDDFFESLSGVCTALDNVEARLYMDQRCLFYGLPMFESGTLGTKGNTQVVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNRYLEGPTFLKELNEQQNTKVETLERLKNSLVDDRPMSFEDCISWARFKFEDLFSNQIKQLLFNFPVDQLTTSGTPFWSGPKRPPTPITFDVKDPLHMDFVVSVANSRAKNYGLKGHADRDSFAQVLASIHVPEFSPRKGVKIAASDAELKE---GGAAPG--LEDVDAQCEFILKELPKPSTLAGYRMQAIEFDKDDDAHMEVIVSVSNLRARSYKIPEEDMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKVFQEKPLDHYKNGFVNLALPLFTFAEPIEPKATKTMLKGEEYKWTAWDRLEVDRGDMTLKEFLAYFEKEYDAEVSMLSYGVTILYAMYSQKSRSKERMAMKISDLVRTVTKKPIDPKLKYLILEVCAMDADGEDV-ELPYLRYHYK 1031          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: A0A5D6Y0E3_9STRA (E1 ubiquitin-activating enzyme n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Y0E3_9STRA)

HSP 1 Score: 1110 bits (2871), Expect = 0.000e+0
Identity = 569/1040 (54.71%), Postives = 715/1040 (68.75%), Query Frame = 2

Query:  197 IEQNGSGVDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQG---MTELEGREFTITEKSPFSFEID-FDTSACGTF------VSGYVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRT-EKGSLPEAGDMDQGEEVYTMAKGFNDEE---GGFKAEG----LDDCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLSKE--EVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            ++ + S +DE LYSRQLYVMGHEAQ RM  +NVL+VG++GLG E+AKN++LAGV+SVTL DD  A   DLSAQFYL+E D+G PRA     KLAELN YV V   SG ++EA +  F+ VV+++A L E  R+N  CHA G+ F+S +ARGVF   FCDFGDAFVVSD DG Q  SC+VAS+      LVTV DD RH L TGDVVTF  + G   + E   R  T+T   PF+F +D  D +    F      V GYV QVK+P T++F  L  ALA+P  F+ +DFAK+ R  +LH AF+ LD +R    G  P  G +  G+EV+ +A   N +    GGF          D K V+  L+ GA GV++P+ A +GGIVGQE LKACSGKF+PI+Q+ Y+DA E LP+   + +  E  PVG RYD  ++VFGK +Q  +    LFLVGAGAIGCEMLKNWAMMG+    +  + VTDMD IEKSNL+RQFLFR  D+ +PKS  A  A+K MNP + ++ Y A+   E+E  F+D+F+  L  VCTALDNVEARLYMDQRCLFY  PM ESGTLGTKGNTQIVVPH TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVNQYL  P++   LN Q NTKLETL R+ +SL   RPS+F+DC+ WAR +F+  F N+I QLLHNFP+D +T++G PFWSG KRPP+P+ FD  DP+H  F+   A  RA  YG+    D +     L  I VPEF P +GVKIA TDAE KE+    +    +L++ DAQ E +L  LP    + +FR+  +EFDKD D HME + A SNLRAR YKIPEADMH+SR IAGKIIPAIATTTALVTGLVC E  K  Q KPL+ YKN F+NLALP F+ SEP+ P  TTT+++G E+KW+AWD L+L   D TL++L    + +Y  E++MLS+GV+ILY+ ++ K +  ERL M++P++VE VTKK + A  +YLI EVC  D D +D  E+PY+R   R
Sbjct:   39 MDVDASAIDEGLYSRQLYVMGHEAQLRMGAANVLVVGLDGLGVEIAKNIVLAGVKSVTLHDDAPASLLDLSAQFYLSERDVGAPRAQVSAKKLAELNPYVHVHAHSGDISEAFLQQFRVVVVVNAALSEAKRINALCHAHGIAFVSTEARGVFGAVFCDFGDAFVVSDRDGEQPVSCMVASIANSTPPLVTVSDDARHQLETGDVVTFREISGFPFLNECAPRRVTVT--GPFTFTLDPLDDADAAQFARGAPSVGGYVTQVKQPLTVAFQRLESALARPGEFLVSDFAKLNRSELLHVAFQALDAYRAAHDGRFPTPGSLADGDEVHALAVALNQQAANVGGFAVPSDLLESADAKKVVRALASGASGVISPMAAFLGGIVGQEALKACSGKFTPIQQFFYFDALECLPDAVFAGDTSEFAPVGSRYDGQLVVFGKMLQATVQNLSLFLVGAGAIGCEMLKNWAMMGVATGANSVIHVTDMDTIEKSNLNRQFLFRSKDVQQPKSTVAGRAIKEMNPDVNVQSYVARVGSESETQFNDEFFESLAGVCTALDNVEARLYMDQRCLFYGLPMFESGTLGTKGNTQIVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNQYLAGPDFVKQLNDQQNTKLETLERLQQSLVDQRPSSFEDCVAWARFKFEDLFSNQIKQLLHNFPLDQVTTTGTPFWSGPKRPPTPVAFDARDPVHLDFIVAVAASRAKTYGLPNHADRARIAKTLATIHVPEFAPREGVKIAATDAEMKEE----AARAPRLDNPDAQAEHLLAALPTAASLSDFRMAPIEFDKDDDAHMEVIVATSNLRARSYKIPEADMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKAFQGKPLDHYKNGFVNLALPLFAFSEPVAPKTTTTMLRGEEYKWTAWDRLDLPAGDLTLKELLAHFEKEYEAEISMLSYGVTILYAMYSQKSRAKERLAMTIPELVETVTKKPLDASLKYLILEVCAMDADGEDI-ELPYVRYHTR 1071          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: K3WKD4_GLOUD (E1 ubiquitin-activating enzyme n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WKD4_GLOUD)

HSP 1 Score: 1103 bits (2852), Expect = 0.000e+0
Identity = 570/1041 (54.76%), Postives = 718/1041 (68.97%), Query Frame = 2

Query:  188 ASGIEQNGSGVDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQG---MTELEGREFTITEKSPFSFEIDF-DTSACGTFVSG------YVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTE-KGSLPEAGDMDQGEEVYTMAKGFNDE---EGGFKAEGLD--DCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLS--KEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            A  +++ G  +DESLYSRQLYVMGHEAQ RM  SNVLI+G+NGLG E+AKN+ILAGV+SVTL DD  A   DL+AQFYLTE D+G+ RA A   KLAELN YV V   SG +TE  +  F+AVV++++ L+E LR+N  CH   + F+S +ARGVF   FCDFGD FVVSD DG Q  +C+V S++     LVTV DD RH L TGD VTF  V+G   + E + R  T+T   PF+F +D  D      F +G      YV QVK+P T+ F PL EALA P  F+ +DFAK  RP +LH AF+ LD ++ + +G  P+ G +   +EVY +A   N E    G F  + L+  + K VI  L+ G+ GV++P+ A +GGIVGQE LKACSGKF+PI+Q+ Y+DA E LP+   S   +E  P G RYD  I+VFGKT+QE +    LFLVGAGAIGCEMLKNWAMMG+    D  + +TDMD IEKSNL+RQFLFR  D+ + KS  A  ++K MNP + +K Y A+   ++E  F+D+F+  L+ VCTALDNVEARLYMDQRCLFY  PM ESGTLGTKGNTQIVVPH TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVNQYL  P +   LN Q NTKLETL R+ +SL   RP++F+DCI WAR +F+  F N+I QLL+NFP+D +T+SG PFWSG KRPP+ + FDV+D +H  FV   A  RA NYG+K   D      VL  I VP F P DGVKIA +DAE KE          ++++ DAQ E +L  LPK + +  +R+E +EFDKD D HME + A SNLRAR YKIPEADMH+SR IAGKIIPAIATTTALVTGLVC E  K  Q KPL+ +KN F+NLALP F+ SEP+ P  T T++KG E+KW+AWD +E++  D TL++       +YG EV+MLS+GV+ILY+ ++ K +  ER+ M + ++VE VTKK I    +YLI EVC  D D +D  E+PY+R   +
Sbjct:   28 AMDVDEAGPKIDESLYSRQLYVMGHEAQMRMGASNVLIIGLNGLGVEIAKNIILAGVKSVTLHDDTPASHLDLAAQFYLTEEDIGQSRAQASSKKLAELNPYVHVQHHSGEVTEDFLKQFRAVVLVNSTLKESLRINQICHVHSIAFVSTEARGVFGSVFCDFGDEFVVSDRDGEQPVNCMVTSISNTTPPLVTVSDDTRHQLETGDYVTFREVKGFPFLNECKPRRVTVT--GPFTFTLDILDEEDEKLFANGSPSAGGYVTQVKQPLTVKFKPLTEALAAPGEFLISDFAKFNRPELLHFAFQALDAYQAKHQGGYPKPGSVQDADEVYAIAVNLNKEAAASGRFSIDHLETEEAKKVIRGLASGSSGVISPMAAFLGGIVGQEALKACSGKFTPIQQFFYFDAVECLPDSVYSGNTDEFAPTGSRYDGQIVVFGKTLQEKIRNLSLFLVGAGAIGCEMLKNWAMMGVSTGKDSVIHITDMDTIEKSNLNRQFLFRSKDVQQAKSTVAGRSIKEMNPEVNVKSYVARVGADSEAQFNDEFFESLSGVCTALDNVEARLYMDQRCLFYGLPMFESGTLGTKGNTQIVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNQYLSGPEFIKQLNDQQNTKLETLERLKQSLVDQRPASFEDCISWARFKFEDLFSNQIKQLLYNFPLDQVTTSGTPFWSGPKRPPTAITFDVNDSVHLDFVVAVANSRAKNYGLKGHHDREAIAKVLSGIHVPAFAPRDGVKIAASDAEMKEDSN-----ASRVDNPDAQAEYILNALPKPDSLAGYRMEPIEFDKDDDSHMEVIVATSNLRARSYKIPEADMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKAFQDKPLDHFKNGFVNLALPLFTFSEPIEPKTTKTMLKGEEYKWTAWDRIEVDIGDITLKEFLAYFDKEYGAEVSMLSYGVTILYAMYSAKSRSKERMAMKISELVETVTKKPIDQNLKYLILEVCAMDADGEDI-ELPYLRYHYK 1060          

BLAST of mRNA_P-fluviatile_contig12.1356.1 vs. uniprot
Match: A0A3R7H2J5_9STRA (E1 ubiquitin-activating enzyme n=4 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7H2J5_9STRA)

HSP 1 Score: 1098 bits (2841), Expect = 0.000e+0
Identity = 568/1042 (54.51%), Postives = 722/1042 (69.29%), Query Frame = 2

Query:  188 ASGIEQNGSG-VDESLYSRQLYVMGHEAQRRMATSNVLIVGVNGLGAEVAKNVILAGVRSVTLLDDGNAEWTDLSAQFYLTEADLGKPRAAACVAKLAELNRYVSVSTASGPLTEALVSSFQAVVMIDAPLEEQLRVNDFCHAKGVCFISCDARGVFAYAFCDFGDAFVVSDVDGNQAASCVVASVTKDPVGLVTVMDDQRHNLVTGDVVTFNSVQG---MTELEGREFTITEKSPFSFEIDF-DTSACGTFVSG------YVNQVKKPSTLSFLPLREALAKPEPFMETDFAKIGRPGVLHQAFRGLDKFRTE-KGSLPEAGDMDQGEEVYTMAKGFNDEEGGFKAEGLD-----DCKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPEKPLS--KEEVQPVGCRYDASIMVFGKTMQELLGKQKLFLVGAGAIGCEMLKNWAMMGIGCSGDGQVFVTDMDHIEKSNLSRQFLFRESDIGRPKSLTAAEAVKAMNPSLKIKPYEAKCAQETEELFSDDFYSGLTAVCTALDNVEARLYMDQRCLFYQKPMLESGTLGTKGNTQIVVPHLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLDDPNYTAFLNSQHNTKLETLTRISESLTSSRPSTFDDCIEWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPSPLDFDVSDPLHTGFVKGAAVLRALNYGIKPPTDDSVYTTVLGKIKVPEFKPLDGVKIATTDAEAKEQGQGGSXXGGQLEDVDAQCERMLRELPKREDMKEFRLEVVEFDKDLDDHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEVYKIMQKKPLESYKNWFLNLALPQFSCSEPLPPAKTTTIIKGSEWKWSAWDSLELEGADTTLQQLFDIMKDKYGLEVTMLSHGVSILYSFFANKKKIAERLPMSLPKIVELVTKKEIPALQRYLIFEVCVSDVDTDDEREVPYIRLKLR 3256
            A G++Q G+  +DE LYSRQLYVMG EAQ RM  SNVLIVG NGLG E+AKNV+LAGV+SVTL DD  A   DL++QFYLTEAD+GKPRA   V KLAELN YV V   SG +TE  +  F+AVV++++ L+E  R+N  CHAK + FI+ +ARGVF   FCDFGD FV+SD DG    SC+++S++     LVTV DD RH L TGD+V+F  V G   + +   R+ T+T   PF+F +D  D +    F  G      YV QVK+P    F  L+ ALA P  F+  DFAKIGR  +LH AF+ LD F+ + +G  P+ G M+  EEV  +A   N +    K   +D     + K VI  LS G+ GV++P+ A +GGIVGQEVLKACSGKF+PI+Q+ Y+DA E LP+   +   EE    G RYD  I+VFG+ MQ+ + K  +FLVGAGAIGCEMLKNWAMMG+    D  + +TDMD IEKSNL+RQFLFR  D+ + KS  AA A+K MNP + +  Y ++   E+E+ F+DDF+  L+ VCTALDNVEARLYMDQRCLFY  PM ESGTLGTKGNTQIVVPH TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVN+YL+ P++   LN Q NTK+ETL R+  SL   RP +F+DCI WAR +F+  F N+I QLL+NFP+D +T++G PFWSG KRPP+P+ FD+ DPLH  FV   A  RA NYG+K   D + +  +L  I VP+F P  GV+IA +DAE KE    G+  G  LED DAQCE +L++LPK   +  +R++ +EFDKD D HME + + SNLRAR YKIPE DMH+SR IAGKIIPAIATTTALVTGLVC E  K+ Q KPL+ YKN F+NLALP F+ +EP+ P  T T++KG E+KW+AWD LE++  D TL++     + +Y  EVTMLS+GV+ILY+ ++ K +  ER+ M +  +V  VTKK I    +YLI EVC  D D++D  E+PY+R   +
Sbjct:   49 AMGVDQTGTATIDEGLYSRQLYVMGREAQVRMGESNVLIVGQNGLGVEIAKNVVLAGVKSVTLHDDTPASSLDLASQFYLTEADIGKPRATVSVQKLAELNPYVPVRCHSGTITEDFLHGFRAVVLVNSSLKEAKRINAICHAKSIAFITTEARGVFGSIFCDFGDEFVISDRDGVDPVSCLISSISDSTPPLVTVSDDARHGLETGDLVSFREVPGFPFLNDSNPRKVTVT--GPFTFTLDIVDDADKFRFAQGKPSSGGYVTQVKQPLVTKFKDLKNALAAPGEFLINDFAKIGRSELLHVAFQALDAFQEKHQGIYPKPGCMEDSEEVIALALELNKQSAAKKEFIVDNITTAESKKVIQALSAGSTGVISPMAAFLGGIVGQEVLKACSGKFTPIQQFFYFDAVECLPDAVYAGTPEEFAAAGSRYDGQIVVFGQAMQDKIKKLNMFLVGAGAIGCEMLKNWAMMGVASGKDSAIHITDMDTIEKSNLNRQFLFRSKDVQQAKSSVAARAIKEMNPDINVHAYVSRVGAESEDQFNDDFFESLSGVCTALDNVEARLYMDQRCLFYGLPMFESGTLGTKGNTQIVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGDFFQAPSDVNRYLEGPDFMKELNEQQNTKVETLERLKNSLVDDRPMSFEDCISWARFKFEDLFSNQIKQLLYNFPLDQVTTTGTPFWSGPKRPPTPITFDLKDPLHMDFVVAVANSRAKNYGLKGHNDRTAFAHILNGIHVPDFSPQKGVRIAASDAELKE----GAPPG--LEDADAQCEFILKDLPKPSTLAGYRMQPIEFDKDDDSHMEVIVSVSNLRARSYKIPEEDMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKVFQDKPLDHYKNGFVNLALPLFTFAEPIEPKATKTMLKGEEFKWTAWDRLEVDRGDITLKEFLTYFESEYDAEVTMLSYGVTILYAMYSQKSRSKERMAMKISDLVRTVTKKPIDPKLKYLILEVCAMDADSEDV-ELPYLRYHYK 1081