mRNA_P-fluviatile_contig1.478.1 (mRNA) Porterinema fluviatile SAG_2381

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1196 bits (3095), Expect = 0.000e+0
Identity = 624/964 (64.73%), Postives = 731/964 (75.83%), Query Frame = 2

Query:   62 PSLGVLETALLTTAKGEFGGDQRTFEAAAARTADR--NHQHHTRAPHLCCTDYDHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEHEENPRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTP-----PVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDYFRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAEPATCEAGKVVSCVGGCIDGSAWYATDDLVTTSTATFLDIDRLCPAFG---CDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEERTSCTISSPAITKNVLAVGATTSGNTRLSTT-GLVDPEVDDSGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGDGFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDVESGVWVVWVWAKRLTTDTQSYSLVVNGAISTAT 2920
            PSL ++E AL TTAK +F G  R FEAAA    +R  + Q  TRAPHLCC +Y HGRE +SRL+  LS EAVR V HSE  GAC+  + +HA A++I ++  Q+ L SF+PFPSALK+APGL++H +    D  T+  L R L ARHG+ MR  +VEGLS+EL PG L + S  A  F+ D L DL+  S+DLH+TNFWSD  +  GE+ +  GGAVR RDWSKAA+VV  LSE+A TSP DICSWN V VHHA  D+LLVSGLDHLL SGRGAGGR   GE+AELH+AC+MGL+SFLA +PEV+RVAPR +Q LLNASARANIQSATV  TPLTDAGLDGTG+V+Q+IDSG+DESSC+FA+DE+G  V HG+Y++EWG N  LT      P + DDD+   WP A        TGS YY  A+ F GGDFT++P+RRKIIQYINL+KSDD  GS+GD F +S GQ +PWLPA+ FEYD  AGHGTH AGS AGATL  PAE  +C+  + +SCVG C+D       DDL T +    +D+DRLCP F    CD    E+CLSDDV+ETLTNNGGMAQGAKLAIFD FY  FGL   VGNGLWEPCAEA CK+HSNS+G D +C LGP DVLYD +M  NPENLLIFAAGN G+  R++CT+SSPAI KNVLAVGA+TSG+TRLS T GL        GIDTVA FSS GPT+D RIKPEV+APGD IYSAASDGS DS++CRLWAYAGTSMS PIVAGA+AM+RQYF++  F++ D+ ARG C +   CE FSPSSAT+KAL+INSANLMGGSSEPDGSRGFGRIHL+AGMPL G GSL LFV+DS  T IPE TRQEY F V+A A  D R TLSW+DPPATTFS VQLVHDLDL V+SP G R+TMWASGVTD+ N NERVIVDA DVE+G W VW+WA  LTTD QSYSLVVNGAIS  T
Sbjct:   22 PSLELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDR-LSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSL-GWPRATDD---HRTGS-YYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVE-IVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGL--------GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGT 970          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1077 bits (2784), Expect = 0.000e+0
Identity = 587/964 (60.89%), Postives = 695/964 (72.10%), Query Frame = 2

Query:   62 PSLGVLETALLTTAKGEFGGDQRTFEAAAARTADR--NHQHHTRAPHLCCTDYDHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEHEENPRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDY--FRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAEPATCEAGKVVSCVGGCIDGSAWYATDDLVTTSTATFL-DIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEERTSCTISSPAITKNVLAVGATTSGNTR-LSTTGLVDPEVDDSGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGDGFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDVESGVWVVWVWAKRLTTDTQSYSLVVNGAISTATVEAAS 2935
            PSL ++E  L TTAK +F G  R FEAAA    +R    Q  TRAPHLCC +Y HGRE +SRL+  LS +AVR V HSE HGAC+L T +HA A++I ++  Q+ L SF+PFPSALK+APGL++H E+   +   S    R LGARHG  MR  +VEGL+V+L P  LPA SAGA  F+ D+L DL+S S+DLH+TNFWSDP +  GE  +  GGAVR RDWSKAA+VV  LSE+A TSP DICSW+ V +  AGDD LLV+GLDHLLFSGRGAGG  HE E+ ELHVAC+MGL+SF A RPEV+RV+PR+ + +LN +AR  IQSA+   TPLTDAGLDGTGEVIQ++D+GLDE+SC+F  D+ G +V HGY+++E                               STGSS      VF  GDFT    RRK+IQYI LV  D       DY  F ++ G ++P+LP ++F  DD AGHGTHTAGSAAGATL  PAE  TC   + + CVGGCID S    TDDLV+ +    L D+DRLCP +GCD S  + CLSD+V ETLT NGGMAQGAKL+IFD F+  FGL+ V+GNG+WEPC EAGCK+HS S G D  C  G +++LYDDFMY+NPENLLIFAAGN G+ ER+ CT++SPAI KNVLAVGA+TSG+TR +ST G        +GID VA FSS G T DGRIKPEVVAPGDM+YSAASDG+ DS++CRLWAY GTSMSCPIVAGAAAMVRQYFVD  FY+ADV ARG C DGF CEGFSPS+ATVKALLINSANLMG SS+PD  RGFGRIHL+ GMPL G G L LFVAD+ DT I E T QEY F VDA A  + RATLSWID PAT+ S +QLV+DLDL VVSP G  + MW S VTD  N NERVIVDA DVE+G W V V++  L TD+QSYSLVVNGAIS  T E AS
Sbjct:   32 PSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDR-LGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGS-HEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFI-DDGGEEVDHGYFFEELFVG---------------------------STGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVNPDS------DYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESE--TTDDLVSYAIQIALPDLDRLCPMYGCDGST-DPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVISTEG-------GAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGT-DSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGTGEGAS 948          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1041 bits (2692), Expect = 0.000e+0
Identity = 576/982 (58.66%), Postives = 677/982 (68.94%), Query Frame = 2

Query:    5 VFVASAIGNGGSLSRVSTLPSLGVLETALLTTAKGEFGGDQRTFEAAAARTADR--NHQHHTRAPHLCCTDYDHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEHEENPRGDDATSKSLGR-RLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQD-GEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDYFRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAEPATCEAGKVVSCVGGCIDGSAWYATDDLVTTSTATF-LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEERTSCTISSPAITKNVLAVGATTSGNTRLSTTGLVDPEVDDSGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGDGFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDVESGVWVVWVWAKRLTTDTQSYSLVVNGAISTATVEAAS 2935
            VF+ S+    G L+     P+L ++E ALLTTAK +F G    FEAAA    +R  + Q  TRAPHL C  Y  G EA SRL+  LS EAVR V HSE HGAC+  + +HA A +I ++  Q+ L +F+PFPS LK+APGL++H E+    +  ++S G  RL ARHG+ MR+ +VEGLSVEL PG L A S+ A  F+  ML DL+S S+DLH+TNFWSDP +   GE  +   GAVR RDW KAA+VV  LSE+AST P DICSWN + VHHAGDD LLVSGLDHLLFSGRGAGG  HE E+ ELHVAC+MGL+SFLA RPEV+RV+PR  + +LNA+AR  IQSA+   TPLTDAGLDGTGE+IQ+IDSGLDE+SCYF  D++G +V HGYY+DE G     +   ++D+                        TA +F GGDF+ YP RRK                                          AGHGTHTAGSAAGATL  PAE  TC     +SCVG CID     ATDDLV+ S+ T   D+DRLCP +GCD+S    CLSDDV ETL+NNGGMAQGAKLAIFD FYG +     +GN LWE C EAGCKLHSNS G+D +C +   D+L D FMY+N ENLLIFAAGN G  ER++CTI++P I KN LA+GA+TSG+TR+ +T      V  +GIDTVA FSS G T D RIKPEVVAPGD +YSAASDG+ D+++CRLWAY GTSMSCPIVAGAAAM+RQYFVD  FY+ADV ARG C DGF CEGFSPS+ATVKALLINSANLMGGSSEPDG RGFGR+HL+AGMPL G G LVLFVAD+ DT I   T +EY F VDA A  D RATLSWIDPPAT  S VQLVH+LDL VVSP G  + MW S  TD  N NERVIVDA DV +G W VWVWA  L TD+QSYSLVVNGAIS  T E AS
Sbjct:   12 VFLCSSAVAAGRLNS----PALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGS-HEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFI-DDSGEEVDHGYYFDELGRAAVYSSSYSSDE------------------------TATIFNGGDFSYYPERRK------------------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACID--LLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVIST------VGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGT-DTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTGEGAS 912          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 900 bits (2327), Expect = 1.910e-306
Identity = 520/981 (53.01%), Postives = 631/981 (64.32%), Query Frame = 2

Query:   11 VASAIGNGGSLSRVSTLPSLGVLETALLTTAKGEFGGDQRTFEAAAARTAD---RNHQHHTRAPHLCCTDYDHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEHEENPRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDYFRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAE-PATCEAGKVVSCVGGCIDGSAWYATDDLVTT---STATFLDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEE--RTSCTISSPAITKNVLAVGATTSGNTRLSTTGLVDPEVDD----SGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGD-GFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWA-SGVTDTVNNNERVIVDAKDVESGVWVVWVWAKRLTTDTQSYSLVVNGAI 2908
            VA++   GGS + +   PSLGV+E +LLTTA   F G  + FE AA  T D      + + RAPHL CT+Y+ G E  SRLQ LLS EAVR V HS  HGAC+ VT +HA A ++S N   Y L S +PFPSALK+APG+LEH  +    D  S+S GR L   HG  MR+ +VEGL++EL+PG LPAHS  A  F+ ++L+DL+S+S+DLH  N WSDP   +GE      GAVR+R+WS AA +V  L+ +  TSP DICSW+++ +HHAG+DLLLVSGLDHLL    GA G   + EA EL VAC+MGL+SFL+ R EV RV+PR+A  LLNA AR  IQSAT+   PLT AGLDGTG+VIQ+I                                                                                       QYINL+K+D + G       +SGG    W+  + F+ DD+  HGTHTAGSA GAT+  PAE P TC   +V  CVGGCID       DDL+T    +++   DIDR+CP  GCD+     CLSD V +TL ++GGMAQGAKL+ FD + G       +GNGLWEPC EAGCK+HS S+G+D +C +  TD+LYDDFMY NPENL++ AAGN G+ E  RT CT+ SPAI KN LAVGAT+SG TR+  T       D     S +DTVA FSS GPT DGRIKPEVVAPGD +YSA SDGS D ++CRL A +GTSM+CPIVAGA+AMVRQYF+D  FY AD AARG C D G +CE FSPSSATVKALLINSANLMGGSSEPDG RGFGRIHL+ GMPL G GSL LFVADS+ T +PEL+ Q++ F VDA A  DFRATLSWIDPPA++ S VQLVHDLDL VVSP G  H MWA SG  D+ N NERVI+ A++VESG + V V    L TD+Q+YSLVVNGA+
Sbjct:   16 VAASTPWGGSANSLP-YPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDN-PYYGLTSISPFPSALKVAPGVLEHGVSR---DQASQSPGR-LATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGS--DEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI---------------------------------------------------------------------------------------QYINLIKADSVSGPASS--TTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGS-DVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 888 bits (2294), Expect = 2.050e-302
Identity = 510/957 (53.29%), Postives = 618/957 (64.58%), Query Frame = 2

Query:   11 VASAIGNGGSLSRVSTLPSLGVLETALLTTAKGEFGGDQRTFEAAAARTAD---RNHQHHTRAPHLCCTDYDHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEHEENPRGDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDYFRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAE-PATCEAGKVVSCVGGCIDGSAWYATDDLVTTS---TATFLDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEE--RTSCTISSPAITKNVLAVGATTSGNTRLSTTG----LVDPEVDDSGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGD-GFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWA-SGVTDTVNNNERVIVDAKDVESGVW 2836
            +A+A    G  +     PSL V+E +LLTTA+  F G  + FE AA  T +      + + RAPHL CT+Y+ G +   RLQ LLS  AVRPV HS  HGAC+ VT +HA A ++S +   Y L S +PFPSALK+APG+LEH  +    D  S+S GR L   HG  MR+ +VEGL++EL+PG LPAHS  A  F+ ++L+DL+S+S+DLH  N WSDP   +GE      GAVR+R+WS AA +V  L+ +  TSP DICSW+++ +H+AG+DLLLVSGLDHLL    GA G   + EA EL VAC+MGL+SFL++R EV+RV+PR+A  LLNA AR  IQSAT+   PLTDAGLDGTG+VIQ+ID+GLDE+SC+F +D+ G +V HG+YY+  G                                                                                      +P+     DD+ GHGTH AGSA GAT+  PAE P TC   +V  CVGGCID       DDL T     +   +DIDRLCP   CDD+    CLSDDV +TL ++GGMAQGAKLA FD F         VGNGLWEPC EAGCK+HS S G+D +C +   DVLYDDFMY NPENL++ AAGN GD E  RT CT+ SPAI KN LAVGAT+SG TR++ T     L+D     + +DTVA FSS GPT DGRIKPEVVAPGD IYSA SDGS D+++CRL A +GTSM+CPIVAGA+AMVRQYF+D  FY AD AARG   D G RCE FSPSSATVKALLINSANLMGGSSEPDG RGFGRIHL+ GMPL G GSL LFVADS+ T +PEL  Q++ F VDA A  DFRATLSWIDPPA++ S VQLVHDLDL VVSP G  H MWA SG  D+ N NERVI+ A+DVESG +
Sbjct:   15 LAAASTPWGGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDS-PYYGLTSISPFPSALKVAPGVLEHGVSL---DQASQSPGR-LTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGS--DEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDD-GEEVPHGHYYEALGEI------------------------------------------------------------------------------------IPSSSSARDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGS-DNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTY 878          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 859 bits (2220), Expect = 3.690e-293
Identity = 443/674 (65.73%), Postives = 511/674 (75.82%), Query Frame = 2

Query:  929 MGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQ-------WPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDYFRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAEPATCEAGKVVSCVGGCIDGSAWYATDDLVTTSTATFLDIDRLCPAFG---CDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEERTSCTISSPAITKNVLAVGATTSGNTRLSTTGLVDPEVDDSGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGDGFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDVESGVWVVWVWAKRLTTDTQSYSLVVNGAISTAT 2920
            MGL+SFLA +PEV+RVAPR +Q LLNA+ARANIQSATV  TPLTDAGLDGTG+V+Q+IDSG+DESSC+FA+DE+G  V HG+Y++EWG N  LT    +              W  A  H G      SYY  A+ F GGDFT+YP+RRKIIQYINL+KSDD  GS+GD F +S GQ +PWL A+ FEYDD AGHGTH AGS AGATL  PAE  +C   + +SCVG C+D       DDL T       D+DRLCP F    CD    ++CLSDDV+ETLTNNGGMAQGAKLAIFD FY  FGL   VG GLWEPCA+A CK+HSNS+G D +C LG  DVLYD++M  NPENLLIFAAGN G+  R++CTI SPAI KNVLAVGA+TSG+TRLS T         SGIDTVA FSS GPT+D RIKPEV+APGD IYSAASDGS D+++CRLWAY GTSMS PIVAGA+AM+RQYF++  F++ D+ ARG C +   CE FSPSSAT+KAL+INSANLMG SSEP+GSRGFGRIHL+AGMPL G GSL LFV+DS  T IPELTRQEY F V+A A  D R TLSW+DPPATTFS VQLVHDLDL V+SP G R+TMWASGVTD+ N NERVIVDA DVE+G W VW+WA  LTTD QSYSLVVNGAIS  T
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTG------SYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVE-IADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATP-------GSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGT 660          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 815 bits (2106), Expect = 1.740e-272
Identity = 484/979 (49.44%), Postives = 627/979 (64.04%), Query Frame = 2

Query:   38 SLSRVSTLPSLGVLETALLTTAKGEFGGDQRTFEAAAARTADRNHQHHTRAPHLCCTDYDHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEHE--ENPRG--DDATSKS--LGRRLGARHGSPMRRDDVEGLSVELAPGILPAHSAG---ATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDYFRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAEPATCEAG-KVVSCVGGCIDGSAWYATDDLVTTSTATF-LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIG-DEERTSCTISSPAITKNVLAVGATTSGNTRLSTTGLVDPEVDD----SGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGDGFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDVE----SGVWVVWVWAKRLTT-DTQSYSLVVNGAIS 2911
            ++S  S +PSLG  E  LLTTA  +F  +     A+A +  DR  +    +P+L C ++  GR AY +L+G L A AV+PV  +  HGAC++VT + A A  +S         +F  FP+ALKIAPGLL+H   + P    D AT+ S     RL   HG+ M+ D   GL VEL+PG LP+HS G   A  F++++L+ L S SLDLHA   WSD  ++ G+    A GA+R R+WS+AA +V   SES   +P+ +C W  VVVHHA +D+LLV+GLDHLL+ GR   G   E EAAEL +ACYMGL+SFLA R EV++VA    +  LNA+ARA IQSA+V  TPLTDAGLDG+GEVIQIID+G++E++C+  + + G++V+HGYY+D   ++     P+ T                S S   S +     FTGGDF+   +RRK+IQYI+L+  D   GS      SSG    P++ A+ F  DD  GHGTH A + AG+T++ PAE  +C  G +V+ C GGCI       T+    +S A +  DI+RLCPAFGC     ++CL DD ++TL+ +GGMAQGAK+A  D FYG++    + GNGLWE CA+AGCK+HSNS+G D  CQL   DVLYD++MY+N E+LL+F+AGN G   +R +CTI SP I KNVL VG+T+SG TRLSTT L      D    S ID V+ FS  G T DGRIKPEVVAPGDMIYSA+ +G   S  C L AYAGTS S  + +GAAAMVRQYF D+ FYSADV +RG C  GF C+ FSP + TVKA+LINSAN+MGG +EPD  RGFGRIHL+AGMPL G G + LFVADS+D  +    + ++ F VD  A  + RATLSW DP A++ S  QLV+DLDL V +P G R+ MW     DT N NERVIV A+ V     +  W V V  +  T  DT S+SLVV GAIS
Sbjct:   17 TVSGASQVPSLGFFEQTLLTTAGADFDSEAAFAFASAWQAGDRQGRS---SPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSS---WEDFTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEE-EAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGD-GTEVEHGYYFDFVASS----NPLFT----------------SSSESRSMFKAE--FTGGDFSYDSDRRKVIQYIDLINPDTGDGS------SSG---MPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCI-------TESSTGSSEADYGEDINRLCPAFGCSG---DQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDSS--CELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAIS 944          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 800 bits (2067), Expect = 8.860e-265
Identity = 519/1102 (47.10%), Postives = 640/1102 (58.08%), Query Frame = 2

Query:   32 GGSLSRVSTLPS--LGVLETALLTTAKGEFGGDQRTFEAAA----ARTADRNHQHHTRAPHLCCTDYDHGREAY-SRLQGLLSAEAV--RPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEHEENPRGDDATSKSLGRRLGARHGSPMRR----------------------------------------------DDVEGLSVELAPGILPAH----SAGATFFV------EDMLEDLVSESLDLHATNFWS--------DPAVQDGEEFARAGGA--VRRRDWSKAAAVVQGLSES-ASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEG-EAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSY-YSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPG--------------------------SYGDYFRSSGGQLFPWLPAEDFEYDDIAGHGTHTAGSAAGATLTYPAEPAT------CEAGKVVSCVGGCIDGSAWYATDDLVTTSTATF-------LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGL-MGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEERTSCTISSPAITKNVLAVGATTSGNTRLSTTGLV-DPEVDDS---GIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGDGFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDV---------------ESGVWVVWVWAKRLT-TDTQSYSLVVNGAISTATV 2923
            GG  S   TLP   L V+  ALLTTA   F   Q  F+AA     AR  D        AP+L C   D  R    + L  +L A A+  R VV S +HG C + T T   A SI Q   Q   +S+A FPSALK+APGLL+H      DD        RL   HG  +RR                                                V GL+VELAPG+L A     S+G  ++       ++  E+L+S+SLD+H  +FWS        D A  +G   +  GG   V  R+W++AA V++GL  +    +  D+CSW+ +VV H  DDLL + G+DHLL  GRGAGG  +EG EA EL VAC+M LLSFLA RPEV+RV+P  A  LLNA+A  N+QSA++  +P+TDAGLDGTGEVIQI+D+GLD +SC+F +++ G  V HGYY++E G                       S  GS  T   +    A+VFTGGDF    +RRK +       S D P                           +YG+ + +S G+ +PW+ A +F  DD  GHGTHTAGSAAGA ++ P+   T      CE G+ +SCVGGCID    +  DDL+TT    +       +D+DRLCP   CDD   E CL DD +ETL  +GG+A+GA+LAIFD      G+ + + GNG+WEP  EAG KL SNSWG    C     DVL+DDFMY+N + LL+FAAGN GD+  + CT+ +PA+ KNVLAVGA+TSG  RLS T    DP   DS    ID +AYFSS GPT D RIKPEVVAPGDMIYSAA  G  + YTCRLW YAGTSMS PIVAGAAA+VRQYF +  FY+ DV  RG C D F CE F+PS+ATVKA+LINSANLMGGS+EPDG RGFGR+HL+AGMPLAG G   LFVADSA T   ELT  EY F VDA A  D RATLSWIDPPATTFS VQLVHDLDL+V SP G+R++MW  G  D++NNNERVIV A ++                 G W V VW+KRL   +TQSYSLVV GAI   T+
Sbjct:   18 GGGASAAPTLPHPPLSVIRGALLTTAGVTFR-HQGEFKAAVEWHEARLDDARP-----APYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQG--QQVFSSWAAFPSALKLAPGLLDHPH----DDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGG--NEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVE--------------------ASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDW-SGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGN-YTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFC-DSFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPTL 1082          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 780 bits (2014), Expect = 8.720e-263
Identity = 427/708 (60.31%), Postives = 500/708 (70.62%), Query Frame = 2

Query:  839 SGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDIPGSYGDYFRSSGGQLFPWLPAEDFEYDDIAGHG--------THTAGSAAGATLTYPAEPATCEAGKVVSCVGGCIDGSAWYATDDLVTTSTATF-LDIDRLCPAFGCDDSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFYGEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEERTSCTISSPAITKNVLAVGATTSGNTRLSTTGLVDPEVDDSGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGDGFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSADTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDVESGVWVVWVWAKRLTTDTQSYSLVVNGAISTATVEAAS 2935
            +GLDHLLFSGRGAGG  HE E+ ELHVAC+MGL+S LA RPEV+RV+PR  + +LNA+AR  +QSA+   TPLTDAGLDGTGE+IQ+IDSGLDE+SCYF  D++G +V HGYY+DE       +   ++D+                        TA +F GGDF+ YP RRKIIQYI+LV  D     Y  ++ S G  +  +LP + F  DD A                SAAGATL  PAE  TC     +SCVG CID      TDDLV+ S  T   D+DRLCP +GCD+S    CLSDDV ETL+NNGGMAQGAKLAIFD FYG++     +GN LWE C EAGCKLHSNS G+D +C +   D+L D FMY+N E+LLIFAAGN G EER++CTI++P I KN LA+GA+TSG+TR+ +T      V  +GI+TVA FSS G T D RIKPEVVAPGD +YSAASDG+ D+++CRLWAY GTSMSCPIVAGAAAM+RQYF+D  FY ADV ARG C D F CEGFSPS+ATVKALLINSANLMGGSSEPDG RGFGR+HL+AGMPL G G+LVLFVAD+ DT I E T  EY F VDA A  D RATLSWIDPP+T  S VQLVH+LDL VVSP G  + MW S  TD  N NERVIVDA DV +G W VWVWA  L TD+QSYSLVVNGAIS  T E AS
Sbjct:    4 TGLDHLLFSGRGAGGS-HEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFI-DDSGEEVDHGYYFDELAQAAVYSSLYSSDE------------------------TATIFNGGDFSYYPERRKIIQYIDLVNPDS---PYSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACID--LLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTRVIST------VGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGT-DTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAISPGTGEGAS 673          

BLAST of mRNA_P-fluviatile_contig1.478.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 654 bits (1688), Expect = 2.290e-210
Identity = 440/1059 (41.55%), Postives = 578/1059 (54.58%), Query Frame = 2

Query:   68 LGVLETALLTTAKGEFGGDQRTFEAAAARTADR---NHQHHTRAPHLCCTDYDHGREAYSRLQGLLSAEAVRPVVHSEQHGACYLVTVTHAHATSISQNLRQYRLASFAPFPSALKIAPGLLEH--EENPR---GDDATSKSLGRRLGARHGSPMRRDDVEGLSVELAPGILPA--HSAGATFFVEDMLEDLVSESLDLHATNFWSDPAVQDGEEFARAGGAVRRRDWSKAAAVVQGLSESASTSPADICSWNTVVVHHAGDDLLLVSGLDHLLFSGRGAGGRRHEGEAAELHVACYMGLLSFLADRPEVVRVAPRYAQGLLNASARANIQSATVIYTPLTDAGLDGTGEVIQIIDSGLDESSCYFANDENGSQVKHGYYYDEWGANFDLTPPVATDDDTWWQWPGAKSHAGSESTGSSYYSTAQVFTGGDFTVYPNRRKIIQYINLVKSDDI-------------------PGSYGDY----FRSSGGQLFPWLPAED-----------------------------------FEYDDIAGHGTHTAGSAAGATLTYPA-EPATCEAGKVVSCVGGCIDGSAWYATDDLVTTSTATFLDIDRLCPAFGCD---DSLYEECLSDDVSETLTNNGGMAQGAKLAIFDGFY-GEFGLMGVVGNGLWEPCAEAGCKLHSNSWGSDHDCQLGPTDVLYDDFMYKNPENLLIFAAGNIGDEE----RTSCTISSPAITKNVLAVGATTSG-----------NTRLSTTGLVD--PE-----------------VDDSGIDTVAYFSSTGPTADGRIKPEVVAPGDMIYSAASDGSADSYTCRLWAYAGTSMSCPIVAGAAAMVRQYFVDNGFYSADVAARGLCGD---GFRCEGFSPSSATVKALLINSANLMGGSSEPDGSRGFGRIHLDAGMPLAGNGSLVLFVADSA--DTFIPELTRQEYKFTVDAKAARDFRATLSWIDPPATTFSVVQLVHDLDLAVVSPRGVRHTMWASGVTDTVNNNERVIVDAKDVESGVWVVWVWAKRLTTDTQSYSLVVNGAI 2908
            LG ++    ++A  +F   Q  + AA++   DR   +    + AP++ C DY  GR A + L+   +  AV  V ++E  G C++VT   + AT++ +    + L S AP   +LK+A  LL+H  + +PR    DD         L + +G  M  D+V GLSV L+PG+L A    + A  F+ D   DL SES+++   +FWSD      E         R R+WS+AAAVV GL+  ++ +  +IC +  + + H  DDL+LV G++HLL       G+R     +E   ACYMGLLS LA +PEV+RV+P  +  L NA A A +QSAT   TPL DAGLDGTGEVIQ++DSGLDE+SC+FA+D+ G QV+HG+ +D           V  +DD            G+ ST    YS         F    +RRKI+QYI L K+ D                    P  Y D     +    G+ +    +ED                                   F  D + GHGT TAGSAAGA       E   C   ++  C GGCI  S     D ++  ST    D+D  CP + CD   D    ECLSDD  E L  +GG+A GA++++FD  Y G        GN LW    E G K+H+NSWG    CQL   + LYD FMY+NPE+LLIFAAGN G  E    R +CT+ SPA+ KN LAVGAT+SG           N      GL D  PE                  + +G+DTVA+FSS GPT DGRIKP+VVAPGD + SA+SDG+ D ++C+L A  GTS SCP+VAGAAA+VRQYF D  F++ DV +RG C +     +C  F+PS+ATVKA++INSA+LMGGSSEP+G RGFGR+HL+AGMP+ G G   L V ++A  D  I EL       TVD  A  + RATL+W+DPP T  S  QL HDLDL V++P G ++TMW SGV DTVN  ERVIV  + +ESGVW V V AK L TD Q YSLVV GAI
Sbjct:   14 LGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDV-----TRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLL------PGKRFP---SETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDD-GLQVEHGFLFDG----------VRVEDD------------GNVSTVLGNYS---------FPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASD---VDAMLDDST---FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGT-DGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIADGEITELV------TVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1013