prot_P-lacustris_contig993.16223.1 (polypeptide) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A6H5JG41_9PHAE (PI3K/PI4K catalytic domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JG41_9PHAE)

HSP 1 Score: 1253 bits (3243), Expect = 0.000e+0
Identity = 623/676 (92.16%), Postives = 642/676 (94.97%), Query Frame = 0

Query:    1 MIIQVGNAVAAWIYLWMTFQLGFYESTDLLPRGPRLPPRHEARYLTAFEELMMRWKDSDVELFVPQLAAVLMSKGVPSRNVHITQLVVGRLSAYLQQRCYRNIGLAVQMMWQFEAAGRRPAEDGPAWNALKNSYVLAALNGDNLPEQLRVRRRAYCTDSMAFVDTLMDICDSLRKMPRNRRLGVLDNELGRVNRMLLRRMQTRGASFEIPGDTGTGGASGGGGG---REWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLEGGSAGVTCEDVVNKKTLPQALQEELLRSRGQDVWEDDIQNQDESSSRSYQLEMEEADCLFFDRATSQYLTSGDPSSPHVKNMREALQVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMFPSSDRRMATQQILFRKRFMDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNGYLK 673
            MIIQ GNAVAAWIYLWMTFQLGFYESTDLLPRGPRLPP+HEARYLTAFEELM+RWKDSDVE FVPQLAAVLMSKGVPSRNVHITQLVVGRLSA LQQRC+ NIGLAVQMMWQFEA GRRP EDGP+WNA KNSYVLAALNGDNLPEQLR++RRAYC DSMAFVDTLMDI DSL+K+P  RRL  LDNELGRVNRMLLRRMQTRGASFEIPGDT  G           REWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLEGGSAGVTCEDVVNKKTLPQALQEELLRSRGQDVWED  QNQDE+SS+SYQLE+EEADCLFFDRAT+QYLTSGDPSSPH+KNMREALQVFGTTSA LERMVR QSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVD+IFKREGLSLRLRPY+ILCCG+MKGLVECVTDAKS+DHIKK TAK+GGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDK+ERFRELFLTGFKAVQRHMREIE LIWPMFPSSD RM TQQILFRKRFMDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNGYLK
Sbjct:    1 MIIQAGNAVAAWIYLWMTFQLGFYESTDLLPRGPRLPPKHEARYLTAFEELMLRWKDSDVEFFVPQLAAVLMSKGVPSRNVHITQLVVGRLSANLQQRCFGNIGLAVQMMWQFEAVGRRPQEDGPSWNAFKNSYVLAALNGDNLPEQLRMQRRAYCADSMAFVDTLMDIGDSLKKLPIKRRLSALDNELGRVNRMLLRRMQTRGASFEIPGDTVAGXXXXXXXXXXXREWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLEGGSAGVTCEDVVNKKTLPQALQEELLRSRGQDVWED--QNQDEASSQSYQLELEEADCLFFDRATNQYLTSGDPSSPHIKNMREALQVFGTTSAALERMVRHQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDSIFKREGLSLRLRPYSILCCGEMKGLVECVTDAKSIDHIKKLTAKSGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKYERFRELFLTGFKAVQRHMREIETLIWPMFPSSDHRMTTQQILFRKRFMDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNGYLK 674          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A835ZKD2_9STRA (Pi 4-kinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZKD2_9STRA)

HSP 1 Score: 355 bits (912), Expect = 5.750e-115
Identity = 177/288 (61.46%), Postives = 215/288 (74.65%), Query Frame = 0

Query:  384 VKNMREALQ-VFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMFPSSDRRMATQQILFRKRFMDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNG 670
            ++ MRE L  VF        RM+R  SPFGHLKGWKL+ FIVK+GDD R EALAMQVI LVD I++REGL L LRPYAI C G   GLVEC+TDA+SVD IKK T     ++ +FER+YMG  +GLYGRA DNFVRSLAGY++VTY+LQVKDRHNANIMLDTRGHIIHIDFG+ILG+SPG W HE+APFK T DY++IMGG GS  W +F+ELFL GF+AVQ+++ +I AL+  M P++DRR   +    +KRF D +TD+EI+ L+  S  S  T QYD  Q   NG
Sbjct:    2 MERMREILHHVFANVDEARTRMLRLNSPFGHLKGWKLSTFIVKAGDDVRQEALAMQVISLVDRIWRREGLRLWLRPYAIACVGHEAGLVECITDARSVDEIKKRTPGYVSMQDYFERLYMGPGAGLYGRAQDNFVRSLAGYSVVTYLLQVKDRHNANIMLDTRGHIIHIDFGFILGASPGLWTHETAPFKLTSDYIDIMGGVGSVNWVKFQELFLAGFRAVQKNINDIAALVQVMMPANDRRTLIEISKLKKRFSDLKTDEEILGLIKSSANSVKTWQYDYIQSLQNG 289          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A4D9D0J9_9STRA (PI3K/PI4K catalytic domain-containing protein n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D0J9_9STRA)

HSP 1 Score: 320 bits (820), Expect = 1.510e-96
Identity = 230/663 (34.69%), Postives = 315/663 (47.51%), Query Frame = 0

Query:   57 DSDVELFVPQLAAVLMSKGVPS------RNVHITQLVVGRLSAYLQQRCYRNIGLAVQMMWQFEAA-------GRR--PAEDGPAWNA----LKNSYVLAALNGDNLPEQLRVRRRAYCTDSMAFVDTLMDICDSLRKMPRNRRLGVLDNELGRVNRMLLRRMQTRGASFE-----------IPGD---------------TGTGGASGGGGGREWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLEGGSAGVTCEDVVNKKTLPQALQEELLRSRGQDVWEDDIQNQDESSSRSYQLEMEEADCLFFDRATSQYLTSGDPS--SPHVKNMREALQVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQ--RHMREIEALIWPMFPSSDRRMATQQILFRKRFMDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNG 670
            DS +E  +PQ+A +L    V        RN  I Q V+        ++C  N+ L +++ W   AA        RR  P   G    A    L N+  + A  G   P  LR  R  Y      FV  L+ +   ++ +   RRL  L   +  VN +L R M TRG   E            PG                TG GG++G      WL E  +A  CPEV+ H++HLP+  L    P      R LRL    C +LPS+E+ PY+VVAE+LQT  +                      +  LP  L                                       FD  T   L++ DP    P  +      +VFG      E+ +R  SPFG L+GW+LA FIVK+GDD R E +AMQ+I LV  IFK EGL L LRPY I+C GD  GL+E V DAKS+DHIKK       ++ ++ER+Y G  S  +  A  NF+RSLAGY + TY+LQVKDRHNANI++D +GH+IHIDFG++LG+SPG  + ESAPFK T++Y+E++GG     W+ FR + + GF  +   RH R +  L+         + A     F KR +        + LVD+S  ++ T  YD +Q++ NG
Sbjct:   66 DSQIEECIPQIATLLACTKVDGPPDAMRRNRWIEQQVI--------EKCTSNVHLGLKLFWLLRAALGHLEIPTRRGLPQITGERQRAHLMSLLNTGEILAREGQQSPGCLRPLRARYFAHCTTFVAALVKLSFDVKGVSSVRRLTALHQGIDGVNALLFRLMHTRGEEMEGGPDEASPSPQTPGSRPSASPPQRHGVSAVTG-GGSAGASAHPSWLTEEHIARQCPEVAAHSVHLPM--LDGSSPI----FRFLRLVSAECELLPSRERCPYMVVAELLQTERN---------------------TRCALPPPLA--------------------------------------FDEDT---LSAMDPRLLDPSTRRAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGLYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAVSIFEVPGKAAILDS-FDKRLIYASYAPNALALVDESFDNYRTKMYDSYQKRRNG 650          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: M2XFY2_GALSU (Phosphatidylinositol 4-kinase n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2XFY2_GALSU)

HSP 1 Score: 270 bits (689), Expect = 4.040e-75
Identity = 187/548 (34.12%), Postives = 287/548 (52.37%), Query Frame = 0

Query:  155 YCTDSMAFVDTLMDICDSLRKMPRNRRLGVLDNELGRVNRMLLRRM---QTRGASFEIPGDTGTGGASGGGGGREWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLEGGSAGVTCE----DVVNKKTLPQALQEELLRSRGQDVWEDDIQNQD------ESSSRSYQLEMEEA-DCLFFDRATS----QYLTSG--DPSSPHVKNMREA--LQVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMFPSSDRRM--ATQQIL--FRKRF----MDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNGYL 672
            Y  D + FV  L+ +  + R  P ++R   +   L R+N + LRRM   ++  +  + P    +          EWL +   A     V+  ++HLPI        A +R +R+LR   E   V P++ + P+LV  EV++T+    S  ++ E        E    D+V     P  +   + R R     E    +++      ESS+     + E   + ++ +  T+    +Y+ SG  D   P     R+A  L V+G         +R+ SPFGH  GWKLA FIVK+GDD R E LA+Q+I     IF  E L L +RP+ ++C     GLVE + DA S+  +KK T     +  +FER+Y GK S  + RA  NFV S+AGY++V Y+LQ+KDRHN NIMLD++G +IHIDFG++LG+SPG    ESAPFK T +++++MGG  S+ ++ FREL + GF A ++H  ++  L+  M   +          +L  FR+RF    ++ +    ++ L+D+SV +W T QYD +Q  TNG L
Sbjct:  438 YFNDGLVFVKQLVRLSSAARDAPSDKRYSFIQRGLDRINELFLRRMSGLESHPSPLKPPPQLPS---------LEWLAQQGRA-----VALRSLHLPIAR------AVSRVLRILRFVPEETIVFPTRTRVPFLVFVEVVETNMLCCSQYVFFEHMLVDDYEENDDKDIVAVMESPH-IASHIARKRTLSSEETPPSSKETRSGSVESSTTPSSKQKEHVRNAVYGNSVTAVNMEEYVESGKEDLEDPDSIISRDAALLAVYGEIWHWKAERIRKASPFGHFSGWKLASFIVKAGDDLRQEQLAVQLIAQFRNIFVEEDLELWVRPFTVVCLSADSGLVETLPDAVSLHALKKRTPNFVTLLDYFERVYGGKDSKRFRRAQRNFVESMAGYSLVCYLLQIKDRHNGNIMLDSQGRVIHIDFGFMLGNSPGSIRFESAPFKLTAEFVDVMGGTHSEAFQYFRELMVQGFLAARKHHEKLTTLVEIMIEGTKIPCMHGGHSVLESFRQRFCLGLLENECIHHMLSLIDESVDNWRTRQYDRYQFYTNGIL 964          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A5J4YK21_PORPP (Phosphatidylinositol 4-kinase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YK21_PORPP)

HSP 1 Score: 250 bits (638), Expect = 2.800e-68
Identity = 185/595 (31.09%), Postives = 288/595 (48.40%), Query Frame = 0

Query:  133 SYVLAALNGDNLPEQLRVRRRAYCTDSMAFVDTLMDICDSLRKMPRNRRLGVLDNELGRVNRMLLRRMQTRGASFEIPGDTGTGGASGGGGGREWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLE---GGSAGVTC----------------EDVVNKKTL----PQALQE---------ELLRSRGQDVWEDDIQNQDESSS-RSYQ-------LEMEEADCLFFDRATSQYLTSGDPSSPHVKNM------REALQ--VFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMFPSSDRRMATQ--QILF---RKRFMDFQTDDEIMR----LVDDSVRSWTTGQYDDFQQKTNG 670
            +Y  +  NG  +   ++  R  Y  D ++ +   + +   LR +P   R       L   N +LLRRM     S   P D                +   VAA+    +  A+HLP+   +   PA    +R+LR+  +   VL +K KAPY+V AEV ++        ++ E       G  C                E+ V+  +L    P  ++E         E  +S G+    D   +Q  +++  S+Q       L  E      + R T + + +     P  ++       R A+Q  V+G      ER V   SPFG L    L+ FIVK+GDD R E LA+Q+IR  + IFK E + + +RP++I+C     GLVE V ++ S+ ++KK T     +R +FER Y   ++  +  A+ NFV S+A Y+++ Y LQ+KDRHN N+MLDT GH++HIDFG++L +SPG    E+APFK T + +E+MGG  S  +  F+EL + GF A ++H  ++ AL+  +  S+      Q   +L    R RF+   T+ E +R    L+D+S  +W + QYD FQ  TNG
Sbjct:  337 AYTASESNGPEILLAIKQERFDYFNDILSLIKAFVKLSLRLRDVPSAEREAACHVGLAECNMLLLRRMAGMSTSAVSPFDQD----------HPPPDADAVAAMGESAAKRALHLPLA--RANDPA----LRILRIAEKETIVLSTKTKAPYMVFAEVYKSQMPCHDKHIFCEHLFAAEDGCGCSNETSDVAGPDDASEEEEAVHASSLIEKQPSGVEESDMSTTGLAESTKSLGESSRSDSAAHQASATTPASFQKLADPAILVREHVKKSVYGRKTGESVLARVDEMPDDEDADAFLAKRHAMQFVVYGELWEDRERRVLASSPFGKLPNVHLSAFIVKAGDDLRQEQLAIQLIRQFEWIFKEENVDVFVRPFSIMCVNADAGLVELVPNSASLHNVKKKTPHFTSLRNYFERTYGAPSTKSFRVALRNFVSSMAAYSLICYFLQIKDRHNGNLMLDTDGHLVHIDFGFMLSNSPGAIKFETAPFKLTDELLEVMGGERSPSYLYFKELVVLGFLAARKHSEKVVALVEILLESNSVMPCMQGGSVLLDQLRSRFLVSCTEHECIRSVLALIDESCNNWRSKQYDKFQNWTNG 915          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A7S0ZJ17_9RHOD (Hypothetical protein n=1 Tax=Timspurckia oligopyrenoides TaxID=708627 RepID=A0A7S0ZJ17_9RHOD)

HSP 1 Score: 243 bits (620), Expect = 1.340e-65
Identity = 178/579 (30.74%), Postives = 282/579 (48.70%), Query Frame = 0

Query:  148 LRVRRRAYCTDSMAFVDTLMDICDSLRKMPRNRRLGVLDNELGRVNRMLLRRMQTRGASFEIPGDTGTGGASGGGGGREWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYAS---------DRLYLEGG---SAGVTCEDVVNKKTLPQALQ--EELLRSRGQDVWEDDIQNQDES----------------SSRSYQLEMEEADCLFFDRATSQYL---------------TSGDPSSPHVKNMREALQVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMFPSSDRRMATQ--QILFRK---RFMDFQTDDEIMR----LVDDSVRSWTTGQYDDFQQKTNGYL 672
            L+  R  Y +D +  V +L+ +  S+  +  + RL  L   L +VN +LL+RMQ   A+   P D+              ++   VA +  + +  +IHLP+        A++ A+R+LR+  +   +L ++ K PY++  EV ++    +          D++Y +     S  +   DV  ++T    +   + +  +  + V EDD                        SRS Q  + +   L  +    Q                    D      K +   + V+G      E+ +   SPFG +   KLA FIVK+GDD R E LAMQ+IR    IF+ E + + LRP+ I+C     GLVE V +A S+  +KK       +R +FER Y    +  +  A+ NFV S+AGY++V+Y LQ+KDRHN N+M+D  G+++HIDFG++L +SPG    ESAPFK T + M++MGG  SD ++ F+EL + GF A ++H  +I AL+  +  S+      Q  +IL  +   RF     + E +R    L+D+S R+W + QYD FQ  TNG L
Sbjct:  397 LKQERFDYFSDMLLVVKSLVKLSLSIHDVVASERLNALKRGLNQVNSLLLKRMQGSSAASMTPFDSSL---------ESEMSVQQVAELGEKAAKRSIHLPLSR------ASSPALRLLRINIDETILLSTRTKDPYMLFLEVYESKLLCSDPYIFCEELRDQIYTKESELESNEIKRNDVKTEETQTHRVLKGDSVTENENESVIEDDXXXXXXXXXXXXXXXXXXXXXXPKSRSAQRRLADPAKLVRESVRRQVYGRNVQNLFGRVDLLPVDEDADVIEAKKLTMLVLVYGELWQYKEKRILNSSPFGTIPNTKLAAFIVKAGDDLRQEQLAMQLIRQFQWIFQTENVDVTLRPFNIICVNADAGLVELVPNAVSLHALKKRVPNFTSLRNYFERTYGAPETTSFKLALRNFVMSMAGYSLVSYFLQIKDRHNGNLMIDADGNLVHIDFGFMLTNSPGAIKFESAPFKLTDELMDVMGGEKSDAYKYFKELIVFGFLAARKHSEKIFALVEILLESNSVMPCMQGGKILLEQLQARFFPSLPEHECIRQVLALIDESARNWRSQQYDRFQWYTNGIL 960          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A7J7IGD6_9RHOD (Phosphatidylinositol 4-kinase beta n=1 Tax=Cyanidiococcus yangmingshanensis TaxID=2690220 RepID=A0A7J7IGD6_9RHOD)

HSP 1 Score: 237 bits (604), Expect = 1.080e-64
Identity = 135/299 (45.15%), Postives = 182/299 (60.87%), Query Frame = 0

Query:  385 KNMREA--LQVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMF-----PSSDRRMATQQILFRKRFMDFQTDDEIMR----LVDDSVRSWTTGQYDDFQQKTNGYL 672
            +  REA  L V+G      E  VR  SPFGHL  W+LA FIVK+GDD R E LA+Q+I  +  IF+ E L L LRP+ I+C G+  GLVE + DA SV  +KK T     +R +FER Y    +  + +AV NF  S+AGY++ TY+ QV+DRHN NIML   GH+IHIDFG++LG+SPG    E+APFK + +Y+E+MG   S  +  FRELF++GF A ++H  ++  LI  M      P     MA    L R+R M    + E +R    L+D+S+ +W T QYD FQ  +NG L
Sbjct:  432 RKAREATLLAVYGEMWEWKEARVRVSSPFGHLPTWRLASFIVKAGDDLRQEQLAVQLIDQMLRIFEEESLPLWLRPFTIVCIGNNAGLVETIPDAVSVHSLKKRTPNFISLREYFERAYGAPGTSAFTKAVRNFTESMAGYSLATYLFQVRDRHNGNIMLAASGHVIHIDFGFMLGNSPGSIRFEAAPFKLSPEYLEVMGDIHSKAFHYFRELFVSGFLACRKHYEKLMTLIEIMLEGTRLPCMAGGMAVVDGL-RQRLMLSLPERECIRAVLDLIDESIDNWRTRQYDRFQFYSNGIL 729          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A7S1XGJ5_9RHOD (Hypothetical protein n=2 Tax=Compsopogon caeruleus TaxID=31354 RepID=A0A7S1XGJ5_9RHOD)

HSP 1 Score: 236 bits (603), Expect = 6.860e-64
Identity = 179/551 (32.49%), Postives = 275/551 (49.91%), Query Frame = 0

Query:  148 LRVRRRAYCTDSMAFVDTLMDICDSLRKMPRNRRLGVLDNELGRVNRMLLRRMQTRGASFEIPGDTGTGGASGGGGGREWLNEHDVAAVCPEVSH-HAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLEGGSAGVTCED-------VVNKKTLPQALQEELLRSRGQ---DVWEDDIQNQDESSSRSYQLEM-----EEADCLFFDRATS--QYLTSGDPSSPHVKNMREALQVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMFPSSDR--RMATQQIL--FRKRF----MDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNGYL 672
            +R  R +Y  D++  V  L+     +R++P   R   L   L  +N+ LLRRM   G ++                  E +   DV A   E S+  +IHLP+        A    +RVLR+  +   +L +K KAPY++  EV++T    +   ++ E   A    +        + N++TL  A Q    RS G    +      Q+QD   +R+ Q+       +     F +R       L + D  S  +     A  V+G      E  + + SPF  + G KL  FIVK+GDD R E LA+Q+I     IF+ E +   LRP+ I+C     GL+EC+TDA S+  +KK T K   +R +FER +    S  + +A   F++S+AGY++++Y LQ+ DRHN NIMLD  G IIHIDFG++LG+SPG    E++PFK T + +E+M       +  F+ELF+ GF A ++H  +I  L+  M   +     +A QQ++   R RF    ++     +I+ L+D+S  +W + QYD FQ  TNG L
Sbjct:  310 VRQERFSYFDDTIMIVKQLVKFSLEIREIPIEERPARLKTRLEGINQSLLRRMA--GPNYRCD---------------ELVA--DVIAANGEFSYLRSIHLPLSR------ADCHVLRVLRINPDETIILNTKTKAPYMLFIEVVETKMLNSDSGVFAEHFFAAQYIDQRKKASVALENEQTLTHAPQV-CPRSPGTLFLETMHTTTQSQD-PDARTRQIVKHAVYGDTEHVSFGERLIGFDSILETIDEESEKISRSTFAA-VYGELWKDREERIIKASPFSVIPGTKLMAFIVKAGDDLRQEQLAVQLIAQFKRIFEDEKVDCFLRPFTIMCSNAESGLIECLTDAVSLHGLKKKTPKFTSVRNYFERAFAPVNSIRFQQAQKRFIQSMAGYSLISYFLQILDRHNGNIMLDVTGRIIHIDFGFMLGNSPGAIRFENSPFKLTDELLEVMASRNDQAFNYFKELFILGFLAARKHHEKITTLVEVMIEGTSMPCMLAGQQVVDELRSRFFLGLLERDCIAKILALIDESYNNWRSKQYDRFQYMTNGIL 832          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: M1VC27_CYAM1 (Phosphatidylinositol 4-kinase n=2 Tax=cellular organisms TaxID=131567 RepID=M1VC27_CYAM1)

HSP 1 Score: 239 bits (610), Expect = 1.230e-63
Identity = 137/299 (45.82%), Postives = 184/299 (61.54%), Query Frame = 0

Query:  385 KNMREA--LQVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKREGLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREIEALIWPMF-----PSSDRRMATQQILFRKRFMDFQTDDEIMR----LVDDSVRSWTTGQYDDFQQKTNGYL 672
            +  REA  L V+G   A  E  VR  SPFGHL  W+LA FIVK+GDD R E LA+Q+I  +  IF+ E L L LRP+ I+C G+  GLVE + DA SV  +KK T     +R +FER Y    S    +AV NF  S+AGY++VTY+ QV+DRHN NIML   GH+IHIDFG++LG+SPG    E+APFK + +Y+++MG   S+ +  FRELF++GF A ++H  ++  LI  M      P     MA    L R+R M    + E +R    L+D+S+ +W T QYD FQ  +NG L
Sbjct:  878 RRAREATLLSVYGEMWAWKEARVRLASPFGHLPTWRLASFIVKAGDDLRQEQLAVQLIEQMLRIFEEESLPLWLRPFTIVCLGNNAGLVETIPDAVSVHALKKRTPNFISLREYFERAYGAPPSPALAKAVRNFTESMAGYSLVTYLFQVRDRHNGNIMLAASGHVIHIDFGFMLGNSPGSIRFEAAPFKLSPEYLQVMGDVQSETFCYFRELFVSGFLACRKHHEKLTTLIEIMLEGTRLPCMAGGMAVVDGL-RQRLMLSLPERECIRAVLDLIDESIDNWRTRQYDRFQFYSNGIL 1175          

BLAST of mRNA_P-lacustris_contig993.16223.1 vs. uniprot
Match: A0A7S2KTT8_9STRA (Hypothetical protein (Fragment) n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2KTT8_9STRA)

HSP 1 Score: 231 bits (589), Expect = 1.650e-61
Identity = 201/630 (31.90%), Postives = 288/630 (45.71%), Query Frame = 0

Query:  145 PEQLRVRRRAYCTDSMAFVDTLMDICDSLRKMPRNRRLGVLDNELGRVNRMLLRRMQTRGA------SFEIPGDTGTGGASGGGGGREWLNEHDVAAVCPEVSHHAIHLPIK---VLKPGQPAATRA-----------VRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSYASDRLYLEGGSAGV--TCED-----------------VVNKKTLPQALQEELLRSRGQDVWEDDIQ---NQDESSSRSYQLE------------------MEEADCL-FFDRATSQYLTS--------GDP-----------SSPHVKNMREAL-QVFGTTSAGLERMVREQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDTIFKRE---GLSLRLRPYAILCCGDMKGLVECVTDAKSVDHIKKSTAKNGGIRTFFERMY-------------MGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWN----HESAPFKFTQDYMEIMGGPGSDKWERFRELFLTGFKAVQRHMREI----EALIWPMFPSSD-RRMATQQILFRKRFMDFQTDDE--IMRLVDDSVRSWTTGQYDDFQQ 666
            P  +   R  +  DSM FVD L  I   LR +P   R   L + L  +N+ L RRM TRG       S E  G   T           W   +    V  ++ HH++H P++   V  PG   AT +           VR LR+  ++CRVL S+E+ P+LV  EV++T       RLY      GV  T E+                 ++N       +  EL+R     +  D        DESS  ++                      E  D    FD    QY            DP           S+P V    E L +VFG   A     +R+QSP+ ++KGW+LA FI+K+G+D R EAL MQ++  + T F+ E    L   LRPY I+C G   G+VEC+ D KSV+ +KK T     +  FFER +                T  L  +A DNF+RSL GY++V Y+LQ+KDRHNANI++D  GHI+HIDFG++LG +P         E APFK + ++ E++GG     ++RF E+F   +     H  EI    EA I  +  ++D  R  +  +  R + M+   + +  IM +V+D++ SW T  YD  Q+
Sbjct:  313 PRSISDLRCRHFGDSMHFVDRLTQISLDLRHVPPIHRRTHLQSRLAELNQRLCRRMATRGRISIDVESIEEGGQMFT----------HWSENN----VREDMIHHSVHFPMEPQSVRWPGGNDATNSNFEHPSKRNGVVRTLRVLPDMCRVLDSRERCPFLVRMEVVETGLEANDARLYAADVQPGVGLTIEEALGSTRPYGNSILKDSIMINGGFASCEIPPELMREHAGRMTADTSSPTFQTDESSDNAHTSNDKAVLSSRGGYQGGNDDSYEPPDMHGTFDMVQEQYYDQVHHDMHHQRDPEYYQPSSPTTYSTPSVSMGPELLNRVFGRPWANECESIRKQSPYRNVKGWRLASFIIKAGEDIRKEALVMQIMSKLWTYFQAEIPPHLRPFLRPYTIMCTGGDAGIVECLPDVKSVNEVKKETDGFTTLNDFFERAFGPYPPFQPGTSPRSAPTVSLE-KARDNFLRSLVGYSVVCYILQIKDRHNANILMDREGHIMHIDFGFVLGETPKMGKVPLFSERAPFKLSAEFWEVIGG--WKNFKRFCEMFEAAYAVAASHSDEICSLVEAAIMNVSRNTDLARSLSASVRGRLQMMNDPIEQKRHIMNIVNDAITSWGTSTYDWLQR 925