prot_P-lacustris_contig979.16102.1 (polypeptide) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: D7G0N8_ECTSI (DNA ligase (NAD(+)) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0N8_ECTSI)

HSP 1 Score: 1198 bits (3099), Expect = 0.000e+0
Identity = 827/1308 (63.23%), Postives = 903/1308 (69.04%), Query Frame = 0

Query:   24 MKKSYPVPRT-LLQRPRSRRTPTSLLGISPYSMATATAATPQRFRDLAFAAPNFRSFQLHGPMKSASRRRPRFRAAATTAARTTLWNTHRIPLSAAGAGNSGVGGKAYLVHRRNLAFGPRPEPVPYRRSPDTA------PGSFVTISVGNTNVVSFGGMRGTCRTFRSSESGLLGQEMEWRLRKGVGLHSETGADRLRCRDVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGV----------ADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGP---GMPTRAREAEARAGAT--EGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELGVCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDKAVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGE------------------SAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRIGLPP 1291
            MKK+   PRT LL+RPRSR T + +LGISP    TA  AT QRFR LAFAAP+FR FQ H   +  +RRRP   A +  A     WNTHR+ L  AG G  G G       R++  +G RP    YR  P  A      PG     + G+      GG     RTF S++ G   +  + RLR G G+ +ETG  +LR +DVA L+EAEAAAELEAL+EEIAAHDVRYYLED PS+TDAEYDALRLRNG+IEAAFP LVRS+SPSSRVGAA+   + EA  NQQ+   AA A        GEGGAA         P A  RA RLP V HLRSLGSLDNVFDEEKA EFVDRVRRA DVATLGEGGGA+   G  A D A         +AGVEGV          ADE    AM    I  + P A   G L  G   E R        EL FVAEPKIDGLTCALLYEDGR VRAATRGDG RGEDVTANALALGDA+IP +LPLSS AAAV+EG     +PTR  EA            SS   P GRFEVRGE+ M  EAFE LNEER+AE LPAFASARNAAAGSLRQLDP VTRRRGLRFFAYGAAV   RG              +   +  + A V GD+LASAFGTQ+ALL ALEGWGFEVARPRL PT SADELV FHRR+ R+RSSLGYAVDGVVYKLDDLALQERLGAS RAPRWAVAHKFGAEEGETVL EIVIQVGRTGALTPVAVLEPV+LGG TVSRATLHNARE+EELG+ PGDRV+VRRAGDVIPQVLGLA   PP  D  S G+GD     FAFPT CPACGT VVKEEDKAVTRCPAGLSCPAQ VE LKHFVSKGAFDIQGLGPARL ELYEAGIVR+PVD+LRLR++TA QA+              AP    D   +  EDGLE A  RDG +      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX ILEGREGWGRLSAR VLDAVD+AKD VSLGRFI+SLGVPLVGARMA+VIAEEFN +F AFWGALTR             A L    + + GT           GP   A+A                         E+F+G+QGMGPA+L SLLRFA+A+NGNRPVVEALASEVRF+ARA      RR   GGGE                     AWVEGKTVVFTG+LTRMKRTETQ AARELGARVTGSISRKTD+VVVGLNPGGKLE+ARELGVEV+SEEEWYRRIGLPP
Sbjct:    1 MKKANLTPRTILLRRPRSRPTRSPVLGISP---GTAATATQQRFRGLAFAAPSFRHFQPH---RRINRRRPLPLAPSAAANTKISWNTHRVSLFVAGTGE-GDG-------RQSSGYGFRP--TRYRGLPVPALAGQFGPGGCFGYTFGD------GG-----RTFCSAKRG---RAEKGRLRAGAGVGAETGTVKLRFKDVATLEEAEAAAELEALSEEIAAHDVRYYLEDVPSVTDAEYDALRLRNGEIEAAFPSLVRSDSPSSRVGAASTTTDMEALNNQQELPVAAAAATSD----GEGGAAP------AGPKASKRAARLPAVRHLRSLGSLDNVFDEEKAAEFVDRVRRAADVATLGEGGGADRQAG--AADGAAK----NETKAGVEGVLPXXXXXXQMADEEARTAMP---IGVELPGAADGGALLAGG--EARA-------ELCFVAEPKIDGLTCALLYEDGRFVRAATRGDGERGEDVTANALALGDAVIPHHLPLSS-AAAVAEGADGGAVPTREAEAXXXXXXXXERAPSSPVVPRGRFEVRGEVFMSDEAFERLNEEREAEGLPAFASARNAAAGSLRQLDPAVTRRRGLRFFAYGAAVGQERG--------------RSASEEAAAAAVGGDALASAFGTQDALLTALEGWGFEVARPRLPPTVSADELVEFHRRLARERSSLGYAVDGVVYKLDDLALQERLGASARAPRWAVAHKFGAEEGETVLSEIVIQVGRTGALTPVAVLEPVLLGGATVSRATLHNAREVEELGLRPGDRVKVRRAGDVIPQVLGLAS--PPHPDSSSAGEGDDDSARFAFPTCCPACGTPVVKEEDKAVTRCPAGLSCPAQAVERLKHFVSKGAFDIQGLGPARLLELYEAGIVRSPVDVLRLRSQTAVQAQP------XXXXXXXAPLA--DQRERDVEDGLEGASVRDGNKGEDGGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSILEGREGWGRLSARNVLDAVDRAKDGVSLGRFIYSLGVPLVGARMAEVIAEEFNGEFEAFWGALTR-------------AGLPTPPKVVNGT-----------GPTEPAAA-------------------------EAFQGIQGMGPAMLASLLRFAKAENGNRPVVEALASEVRFSARA------RRRHAGGGEWMVALDAVVXXXXXXXXXXXXAWVEGKTVVFTGTLTRMKRTETQEAARELGARVTGSISRKTDMVVVGLNPGGKLEKARELGVEVVSEEEWYRRIGLPP 1170          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A6H5JUK0_9PHAE (DNA ligase (NAD(+)) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JUK0_9PHAE)

HSP 1 Score: 1051 bits (2719), Expect = 0.000e+0
Identity = 774/1305 (59.31%), Postives = 841/1305 (64.44%), Query Frame = 0

Query:    1 MRHALLLLLTTSLKL-TNYLSEGNMKKSYPVPRTLLQRPRSRRTPTSLLGISPYSMATATAATPQRFRDLAFAAPNFRSFQLHGPMKSASRRRPRFRAAATTAARTTL-WNTHRIPLSAAGAGNSGVGGKAYLVHRRNLAFGPRPEPVPYRRSPDTAPGSFVTISVGNTNVVSFGGMRGTCRTFRSSESGLLGQEMEWRLRKGVGLHSETGADRLRCRDVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGV---------ADEGVGVAMAVNGISTKAPAAVRVG-ILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSS-AAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELGVCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDKAVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGRE-----------------------------------------------------GWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAG----------AWVEGKTVVFTGSLTR 1229
            M    LLLLT SL      LS G MKK+   PRTLL+RPRSR T   +LGISP    TA  AT QRFR LAFAAP FR  Q H  +     RRP    A + AA T + WNTHR+ L  AG G  G         RR+  +G RP    YR  P  AP             V  GG     RTF S+  G   +  + RLR G G+  ET   +LR +D A L+EAEAAAELEAL+EEIAAHDVRYYLED PS+TDAEYDALRLRN +IEAAFP LVRS+SPSSRVG A+   + EA  NQQ+   AA  P       GEGGAA         P A  RA RLP V HLRSLGSLDNVFDEEK  EFVDRVRRA DVATLGEGGGA+   G  A D A  +      +AGVEGV         ADE    AM ++    + P A   G +L GG   ETR        EL FVAEPKIDGLTCALLYEDGR VRAATRGDG RGEDVTANALALGDA+IPR LPLSSPAA            REA A A     A  +   P GRFEVRGE+ M  EAFE LNEER+ E LPAF SARNAAAGSLRQLDP VTRRRGLRFFAYGAAV   RG   +G             +  + A V GD+LASAFGTQ+ALL ALEGWGFEVA+PRL PT SADELV FHRR+ R+RSSLGYAVDGVVYKLDDLALQERLGAS RAPRWAVAHKFGAEEGETVL EIVIQVGRTGALTPVAVLEPV+LGG TVSRATLHNARE+EELG+ PGDRV+VRRAGDVIPQVLGLA    PD    S G        FAFPT CPACGT VVKEEDKAVTRCPAGLSCPAQ VE LKHFVSKGAFDIQGLGPARL ELYEAGIV +PVD+LRLR++TA QAR P               DE + E    EDGLE A  RDG +       XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                                                           GWGRLSAR VLDAVD+AKD VSLGRFI+SLGVPLVGARMA+VIAEEFN DF AFWGALTR   ASDASQALEAAQ   D E    T A+ N    A      A+AXXXXX                 +  E+F+G+QGMGPA+L SLLRFA+A+NGNRPVVEALAS VRF+ARA+R                          AWVEGKTVVFTG+LTR
Sbjct:    1 MHPTFLLLLTASLNCCAACLSVGTMKKANLTPRTLLRRPRSRPTRPPVLGISP---GTAATATQQRFRGLAFAAPRFRHSQSHRRLHC---RRPPLPLAPSAAANTEISWNTHRVSLFVAGTGERGG--------RRSSVYGFRP--TRYRGLPVPAPAGKFDPGECFGYTVGDGG-----RTFCSATRG---RAEKGRLRAGAGVGGETTV-KLRFKDAATLEEAEAAAELEALSEEIAAHDVRYYLEDVPSVTDAEYDALRLRNEEIEAAFPSLVRSDSPSSRVGVASTTNDMEALNNQQELPVAAAVPTSD----GEGGAAR------AGPAASKRAARLPAVRHLRSLGSLDNVFDEEKVAEFVDRVRRAADVATLGEGGGADWQAG--AADGAANSE----TKAGVEGVLPXXXXXQMADEEARTAMPID---VELPGAADGGELLAGG---ETRA-------ELYFVAEPKIDGLTCALLYEDGRFVRAATRGDGERGEDVTANALALGDAVIPRRLPLSSPAAVAEGADAGAVLTREAAAVAXXXXXAERAPVVPRGRFEVRGEVFMSDEAFERLNEEREVEGLPAFTSARNAAAGSLRQLDPAVTRRRGLRFFAYGAAVEQERGRPASG-------------EAAAAAAVGGDALASAFGTQDALLTALEGWGFEVAKPRLPPTVSADELVEFHRRLARERSSLGYAVDGVVYKLDDLALQERLGASARAPRWAVAHKFGAEEGETVLSEIVIQVGRTGALTPVAVLEPVLLGGATVSRATLHNAREVEELGLRPGDRVKVRRAGDVIPQVLGLASRLHPDSS--SAGXXXXDSARFAFPTCCPACGTPVVKEEDKAVTRCPAGLSCPAQAVERLKHFVSKGAFDIQGLGPARLLELYEAGIVGSPVDVLRLRSQTAVQAR-PXXXXXXXXXXXXPLADERERE---LEDGLEGASVRDGNKGKDSGDKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGWGRLSARNVLDAVDRAKDGVSLGRFIYSLGVPLVGARMAEVIAEEFNGDFEAFWGALTR---ASDASQALEAAQHTRDRE----TAAVANT--AAERQETNAAAXXXXXVAAATFAAVNGNGPTEPAAAEAFQGIQGMGPAMLASLLRFAKAENGNRPVVEALASGVRFSARARRXXXXXXXXXXXXXXXXXXXXXXXXXXAWVEGKTVVFTGTLTR 1223          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A4R3MIP5_9HYPH (DNA ligase n=1 Tax=Tepidamorphus gemmatus TaxID=747076 RepID=A0A4R3MIP5_9HYPH)

HSP 1 Score: 450 bits (1158), Expect = 4.160e-139
Identity = 369/1081 (34.14%), Postives = 469/1081 (43.39%), Query Frame = 0

Query:  214 RCRDVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELGVCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKE------EDKAVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRIG 1288
            R + VA L   EAAAELE LA EIAAHD RYY ED P I+DAEYDALR RN  IEAAFP LVR +SPS R+GAA                                               PS   +   V H   + SLDN FDE+    FV+RVR  ++                                                                             LP + E+LF AEPKIDGL+ AL YE+GR V+AATRGDG  GEDVTANA  + D  +P+ L           G G            GA             FEVRGE+ M +  FE+LN  ++AE    FA+ RNAAAGSLRQLDP +T  R LRFFAY                 W     + +R P                TQ  +  A   WG     P +    S  E++AF+R++  DR+ LGY +DGVVYK+D L  Q RLG  +R+PRWA+AHKF A++  TVL +I IQVGRTGALTPVA L+PV +GGV V  ATLHN  EI    V  GD V V+RAGDVIPQ+L +   + P+  V            F FPT CPACG+  V+E       + AV RC  GL CPAQ VE L+HFVS+ AFDI+GLG  ++   +  G++R P DI  L+A+           DA   T +                                                                                          REGWG  SA K+  A+D A+ ++ L RFI++LG+  VG   A ++A  +   F AF               A++AA    +SEA +   AI                                                G+G  +  +L++F   +  N   ++AL +EVR    AQ G            S  + V GKTVVFTG+L RM R E +A A  LGA+V GS+S KTDLVV G   G KL++A ELG+EVI E++W+ R+G
Sbjct:    8 RGKPVAELSREEAAAELEQLAHEIAAHDRRYYQEDAPIISDAEYDALRQRNAAIEAAFPDLVRPDSPSLRIGAA-----------------------------------------------PSE--KFAKVRHKVPMLSLDNAFDEDDVAAFVERVRSFLN-----------------------------------------------------------------------------LPAETEILFTAEPKIDGLSIALRYENGRFVQAATRGDGEEGEDVTANAATIAD--VPKRL----------TGDGF-----------GAV------------FEVRGEVYMSHADFEALNARQEAEGGQLFANPRNAAAGSLRQLDPKITAARPLRFFAY----------------AWG----EADRLPSD--------------TQWGVYEAFRSWGLPT-NPLMRRVRSVPEIMAFYRQIEADRARLGYDIDGVVYKVDRLDWQRRLGFRSRSPRWAIAHKFPAQKAMTVLEDIEIQVGRTGALTPVAKLKPVTVGGVVVRNATLHNEDEIARKDVRIGDTVIVQRAGDVIPQILSVVLEKRPEWAV-----------PFVFPTTCPACGSHAVREVNPRTGREDAVRRCTGGLVCPAQAVEKLRHFVSRNAFDIEGLGEKQIAAFFADGLIRTPADIFTLKAR-----------DAQAETKIA----------------------------------------------------------------------------------------DREGWGPTSAAKLFAAID-ARREIDLHRFIYALGIRHVGETNAKLLARHYGT-FEAF-------------RTAMKAAGAGPESEAWQDLLAI-----------------------------------------------DGIGETVAEALVQFF-GEPHNEAAIDALLAEVRPKPAAQAG------------SVDSPVAGKTVVFTGALERMTRDEAKAMAERLGAKVAGSVSSKTDLVVAGPGAGSKLKKAVELGIEVIDEDQWFARVG 707          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A7X5EZJ6_9HYPH (DNA ligase n=3 Tax=Pannonibacter TaxID=227873 RepID=A0A7X5EZJ6_9HYPH)

HSP 1 Score: 425 bits (1092), Expect = 1.710e-129
Identity = 353/1089 (32.42%), Postives = 464/1089 (42.61%), Query Frame = 0

Query:  217 DVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELGVCP-----------GDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDK------AVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRIG 1288
            D   L   EAAAELE LA EIA HD RY+ ED P+I+DA+YDALR RN +IEA FP LVR +SPS RVGA                                                 S A     + H   + SLDN F +E  R+FV RVRR +                                                                               P   +L+  AEPKIDGL+ +L YE+G LV AATRGDGT GE+VTANA  + D  IP+ LP   PA                                    EVRGE+ M +  F +LN    A     FA+ RNAAAGSLRQL+P++T  R LRFFAY                 W     +M   P +              TQ  ++R    WGF V  PR+   A  +EL+A +  +  DRS LGY +DGVVYK+D L LQ+RLG  +R+PRWA+AHKF AE+  TVL +I IQVGRTGALTPVA LEPV +GGV VS ATLHN   I+ +G+             GD V ++RAGDVIPQ++ +   R P    D++         + FPT CPACG+  V+EE+       +V RC  GL CPAQ  E LKHFV + AFDI+GLG  +++  +  G+VR P DI  L A+  A   +                                                                                                   L  R+GWG +SAR + +A++ A+  + L RFIF+LG+  VG   A ++A  +       WGA           +A   A L H S+A     A                                               V G+G  +  ++++F   D+ NR  + AL +EV           +   +D    ++G+ V GKTVVFTGSL RM R E +A A  LGA+V GS+S+KT+L+V G   G KL+ A++LG+EVI+E+EW+  +G
Sbjct:    8 DADHLTREEAAAELERLAAEIAGHDRRYHAEDAPTISDADYDALRRRNAEIEARFPDLVRPDSPSLRVGA-------------------------------------------------SVADGFGKITHQVPMLSLDNAFTDEDVRDFVGRVRRFLKFD----------------------------------------------------------------------------PLMGDLVVTAEPKIDGLSLSLRYENGVLVSAATRGDGTVGENVTANARTIAD--IPQRLPAGVPAV----------------------------------VEVRGEVYMSHADFAALNARMAANGGKTFANPRNAAAGSLRQLNPEITASRPLRFFAY----------------AWG----EMSEVPAA--------------TQFDMVRQFADWGFAV-NPRMQRCARVEELLAVYHGIEEDRSGLGYDIDGVVYKIDRLDLQQRLGFVSRSPRWAIAHKFPAEKAYTVLKDIEIQVGRTGALTPVAKLEPVTVGGVVVSNATLHNEDYIKAIGMDGEPIRGGKDLRIGDTVMIQRAGDVIPQIVDIDLARRP---ADAQ--------PYVFPTVCPACGSHAVREENAKTGRLDSVRRCTGGLICPAQAAEKLKHFVQRNAFDIEGLGDKQIDAFFAEGLVRTPADIFTLEARDKAALTK---------------------------------------------------------------------------------------------------LRNRDGWGAVSARNLFEAIN-ARRRIDLHRFIFALGIRHVGEGNAKLLARAYGS-----WGAF----------EAAMTAALDHASDAWADLNA-----------------------------------------------VDGIGETLAEAVVQFFAEDH-NREALAALLAEVT--------PLDAEKID----ASGSPVAGKTVVFTGSLERMTRDEAKAMAERLGAKVAGSVSKKTNLLVAGPGAGSKLKTAQDLGIEVITEDEWFDLVG 714          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A6N7ZYQ8_9HYPH (DNA ligase n=1 Tax=Roseibium sp. RKSG952 TaxID=2529384 RepID=A0A6N7ZYQ8_9HYPH)

HSP 1 Score: 424 bits (1090), Expect = 3.620e-129
Identity = 356/1088 (32.72%), Postives = 471/1088 (43.29%), Query Frame = 0

Query:  217 DVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELG-----------VCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDK------AVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRI 1287
            D+  L   +A  ELE LA EIA HD RY+ ED P+I+DA+YDALRLRN  +EA FP LVR +SPS+RVGAA                                               PS A     + H  ++ SLDN F ++  R+FV RVRR +                                                                               P    L   AEPKIDGL+ +L YE G+L+ AATRGDGT GE+VTANA  + D  IP+ L  + P                                     EVRGE+ M +  F++LN    A     FA+ RNAAAGSLRQL+ D+T  R L+FFAY                 W                  GD       TQ  ++R  E WGF V  P +    S +EL+  + R+  +R++LGY +DGVVYK+D L LQ+RLG  +R+PRWA+AHKF AE+  T+L +I IQVGRTGALTPVA LEPV +GGV VS ATLHN   I+ +G           +  GD V+++RAGDVIPQ++          DVD     + G +AF FPT CPACG+  V+EE+       A+ RC  GL CPAQ  E LKHFVS+ AFDI+GLG  +++  Y+ G+V  P DI  L                                    ED  ++A  +                                                               L  REGWG +SAR + +A++ A+ ++ L RFIF+LG+  VG   A ++A  +       WG        SDA  A      AHD    +G  A T++N                                          + G+G  +  +L  F  A+  NR  ++AL  EV          R+   +D    ++G+ V GKTVVFTGSL RM R E +A A   GA+V+GS+S+KTDLVV G   G KL++A ELG+EVISE+ W+ R+
Sbjct:   10 DIDTLTLEQARGELERLASEIAEHDRRYHQEDAPTISDADYDALRLRNAALEARFPELVREDSPSARVGAA-----------------------------------------------PSEAFSK--ITHKVAMLSLDNAFKDDDVRDFVGRVRRFLKYD----------------------------------------------------------------------------PLMGALAVTAEPKIDGLSLSLRYERGKLIYAATRGDGTTGENVTANARTISD--IPQVLTGAVPEV----------------------------------VEVRGEVYMAHADFQALNTRMAANGGKVFANPRNAAAGSLRQLNSDITASRPLKFFAY----------------AW------------------GDMSEIPAKTQLDMVRIFESWGF-VINPLMKRCESVEELLEVYHRIEEERATLGYDIDGVVYKVDRLDLQQRLGFVSRSPRWAIAHKFPAEKAFTILNDIEIQVGRTGALTPVAKLEPVTVGGVVVSNATLHNEDYIKGIGQDGEPIRGGKDLRVGDTVQIQRAGDVIPQIV----------DVDLAKRSE-GAEAFVFPTLCPACGSHAVREENAKSGRMDAIRRCTGGLICPAQATEKLKHFVSRNAFDIEGLGDKQVDAFYQDGLVMAPADIFTL------------------------------------EDRDKQALTK---------------------------------------------------------------LRNREGWGAVSARNLFEAIN-ARRNIDLNRFIFALGIRHVGEGNAKLLARAYGS-----WGVF------SDAMIA------AHD----KGGDAWTDLN-----------------------------------------NIDGIGTIVADALTEFF-AEEHNRAQLDALVKEV--------SPRDVEKID----ASGSPVAGKTVVFTGSLERMTRDEAKAMAERYGAKVSGSVSKKTDLVVAGPKAGSKLKKAEELGIEVISEDAWFERV 715          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A346R4B7_9HYPH (DNA ligase n=1 Tax=Breoghania sp. L-A4 TaxID=2304600 RepID=A0A346R4B7_9HYPH)

HSP 1 Score: 424 bits (1089), Expect = 5.040e-129
Identity = 352/1090 (32.29%), Postives = 461/1090 (42.29%), Query Frame = 0

Query:  217 DVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELG-----------VCPGDRVRVRRAGDVIPQVLGL-AQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDK------AVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRIG 1288
            DV  L   +AAAEL  LAEEI+AHD RYY +D PSI+DAEYDALR RN  IE  FP LVR++SPS +VGAA   A                         G+                         + H   + SLDN F ++  R+FV RVRR +    L                                                  K P AV                           AEPKIDGL+ +L +E GRLV AATRGDG  GE+VT NA  + D  IPR LP   P                                     EVRGE+ M +  F +LNE         FA+ RNAAAGSLRQL+ ++TR+R LRFFAY                 W                  G+  A    TQ  ++     WGF    P +    S +EL+  +  +   R++LGY +DGVVYK+D L LQ+RLG  +R+PRWA+AHKF AE+  T+L  I IQVGRTGALTPVA LEPV +GGV VS A+LHN   I  +G           +  GD VR++RAGDVIPQ++ L    RPPD +             FAFPT CPACG+  V+E         A+ RC  GL CPAQ  E LKHFVS+ AFDI+GLG  +++  Y  G+V+NP DI  LR + AA   R                                                                                                   L+ ++GWG  SA+ + DA+D+ ++ + L RFI++LG+  VG   A ++A  +       W A  R  RA++               A +    + NI+GV                                            G  +  +L++F  ++  NR V+EAL +EV      +  D             G+ V GKT+VFTGSL RM R E +A A   GA+V GS+S+KTDLV+ G   G KL++A ELGV+VISE++W+  +G
Sbjct:   10 DVDDLTPEQAAAELARLAEEISAHDARYYRDDAPSISDAEYDALRQRNAAIEKRFPDLVRADSPSVKVGAAPQEA------------------------FGK-------------------------ITHAIPMLSLDNAFSDDDVRDFVGRVRRFLKYDPL--------------------------------------------------KGPLAV--------------------------TAEPKIDGLSLSLRFEQGRLVSAATRGDGATGENVTRNAETIDD--IPRSLPKDVPDI----------------------------------VEVRGEVYMAHADFTALNERMVGNGGKPFANPRNAAAGSLRQLNVEITRQRPLRFFAY----------------AW------------------GEMSAMPADTQMGMIDVFSQWGF-ATNPLMKRCESVEELIDVYHHIEETRATLGYDIDGVVYKIDRLDLQKRLGFVSRSPRWAIAHKFPAEKAFTILNAIEIQVGRTGALTPVAKLEPVTVGGVVVSNASLHNEDYIRGVGQDGEPLRGGKDIRVGDTVRIQRAGDVIPQIIDLDLTKRPPDAEP------------FAFPTTCPACGSHAVREVHPKTGRIDAIRRCTGGLICPAQATEKLKHFVSRNAFDIEGLGDKQVDAFYADGMVKNPADIFTLRRRDAASLTR---------------------------------------------------------------------------------------------------LKNKDGWGETSAKNLFDAIDERRE-IDLHRFIYALGIRHVGEGNAKLLARAYGS-----WEAFARAMRAAE-------------DHAGDAWAELNNIDGV--------------------------------------------GEVVAEALVQFF-SEPHNREVLEALLAEVTPVDAEKIAD------------TGSPVAGKTLVFTGSLERMTRDEAKAMAERFGAKVAGSVSKKTDLVIAGPGAGSKLKKAEELGVDVISEDDWFDLVG 716          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A117S6A0_9PROT (DNA ligase n=1 Tax=Alphaproteobacteria bacterium BRH_c36 TaxID=1734406 RepID=A0A117S6A0_9PROT)

HSP 1 Score: 416 bits (1069), Expect = 2.940e-126
Identity = 350/1074 (32.59%), Postives = 446/1074 (41.53%), Query Frame = 0

Query:  221 LDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELGVCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDK------AVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRIG 1288
            L E EA  EL  LA EIA HD  YY  D P I+DA+YDALRLRN  IEA FP LVR++SPS +VGAA V                           G G                        V H   + SL N F  E   +FV R+RR                                                                   LG G              E+ F AEPKIDGL+ +L Y+ GRLV AATRGDGT GE+VTAN   + +  IP  L          +G  +P                          ++RGEI + +  F  LN E+ A     FA+ RNAAAGSLRQLD ++T +R LRFFAYG                W                  G++      TQ  +L A + WG     P      SADEL+ ++R +   R+SLGY +DG+VYK+D L LQERLG  +RAPRWA+AHKF AE+  T+L +I IQVGRTGALTPVA LEPV +GGV VS ATLHN  EI    V  GD V V+RAGDVIPQ++ +   R P+             + F FP  CP CG+  V+E D+       V RC  GL CPAQ VE LKHFVS+ AFDI GLG   +   Y+ G++++P DI  L  + AA   R                                                                                                   L+ REGWG  SA+K+  A+D A+ ++ L RFI+SLG+  VG   A V+A  +  D   F  A+                  A + E  E    + NI+G+                                            G  +  +L+ F  A+  N  VVE L +EV  T       R+              V GKTVVFTGSL +  R+E +A A  LGA+V GS+S+KTD VV G + G KL +ARELG+ V++E+EW + I 
Sbjct:   16 LSEEEARGELAWLAAEIARHDALYYRSDAPEISDADYDALRLRNDAIEARFPELVRADSPSLKVGAAPVD--------------------------GFGK-----------------------VRHRVPMLSLGNAFSAEDITDFVARIRR------------------------------------------------------------------FLGLGP-----------DAEIAFTAEPKIDGLSISLRYDKGRLVEAATRGDGTEGENVTANVSTMNE--IPHRL----------KGDDLPDT-----------------------IDIRGEIFISHADFARLNAEQAAAGHKVFANPRNAAAGSLRQLDSNITAKRPLRFFAYG----------------W------------------GEAKGLPADTQSGVLAAFDRWGMPT-NPETTVCQSADELMDYYRALEVKRASLGYDIDGIVYKVDRLDLQERLGFVSRAPRWAIAHKFPAEQATTILKDIEIQVGRTGALTPVAKLEPVTVGGVVVSNATLHNEDEIARKDVRVGDTVIVQRAGDVIPQIVSVVLERRPENS-----------EPFRFPVVCPVCGSHAVREADEKSGRTDVVRRCTGGLICPAQRVERLKHFVSRNAFDIDGLGDKGIQAFYDDGLIQSPPDIFTLEKRDAAAPER---------------------------------------------------------------------------------------------------LKDREGWGEQSAKKLFAAID-ARREIPLDRFIYSLGIRHVGETTAKVLARSYG-DIETFKAAMIA----------------AGEGEESEAWADLNNIDGI--------------------------------------------GKTVAEALVEFF-AEPHNVGVVEDLLNEVTPTPLEAVASRSP-------------VAGKTVVFTGSLEKFSRSEAKAKAERLGAKVAGSVSKKTDYVVAGADAGSKLTKARELGIAVLTEDEWEQLIS 707          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: UPI0003F837DE (NAD-dependent DNA ligase LigA n=1 Tax=Azospirillum halopraeferens TaxID=34010 RepID=UPI0003F837DE)

HSP 1 Score: 418 bits (1074), Expect = 5.860e-126
Identity = 360/1104 (32.61%), Postives = 457/1104 (41.39%), Query Frame = 0

Query:  213 LRCRDVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELGVCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDKAVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFN------QDFHAF--------WGALTRQSRASDAS-----------QALEAAQLAHDSEALEGTKAITNINGVAS--GPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEAL-ASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRIG 1288
            LR   V  L   +A AEL  LA EIA HD  Y+ +D P ITDA+YD L  RNG IEA FP L RS+SPS RVGAA                PAA          G G                        V H   + SL N F  E+  EFV RVRR + +                  DNAP+A                                                            F AEPK+DGL+C+L YEDGRLV AATRGDG  GEDVTAN   + D  +P  LP   PA                                    EVRGE+ M  + F ++N  R   D P FA+ RNAAAGSLRQLDP +T +R L FF Y     S R                           V D       TQ  + R L+GWGF + RP  A     + L+ ++  + R R++L + +DGVVYK+D + LQERLG  +RAPRWA+AHKF AE+ +T L  I IQVGRTGALTPVA LEPV +GGV VSRATLHN  EI    +  GD V V+RAGDVIPQV+G+   + P              ++F FP  CP CG+  V+E D+AV RC  GL CPAQ  E L+HFVS+ AFDI+GLG   + E    G+VR+P DI  L+ +                                                                                                          L GR GW   S   +LDA+++ ++ ++L RFIF+LG+  VG   A  +A  +       +   A         W  L        AS           + LE  +   ++  L    A+ N+ G+     P + A                                V+ +G  +   +  F   D  N  V+E L A+ VR      R            ++A + V GKTVVFTG+LT M R E +A A  LGA+V GS+S+KTD VV G + G K  +ARELGV V+SEEEW    G
Sbjct:   11 LRAIPVDDLTIEQAQAELAVLAAEIARHDALYHQQDRPEITDADYDRLVRRNGAIEARFPELQRSDSPSLRVGAA----------------PAA----------GFGK-----------------------VRHAMPMLSLGNAFAPEEVAEFVARVRRFLSLP-----------------DNAPLA------------------------------------------------------------FEAEPKVDGLSCSLRYEDGRLVLAATRGDGAEGEDVTANVRTIRD--VPAELPPPFPAV----------------------------------LEVRGEVYMTRDDFLAMNRARAERDEPPFANPRNAAAGSLRQLDPSITAQRPLCFFGYALGETSER---------------------------VAD-------TQSGIRRRLKGWGFRLNRPA-ARLGGVEALLRYYAGIGRRRAALPFDIDGVVYKVDAIDLQERLGFVSRAPRWAIAHKFPAEQAQTRLKAIAIQVGRTGALTPVAELEPVTVGGVVVSRATLHNEDEIARKDIRVGDLVTVQRAGDVIPQVVGVILSQRPHES-----------ESFVFPDHCPECGSLAVREPDEAVRRCTGGLICPAQAKERLRHFVSRNAFDIEGLGEKIIEEFRNDGLVRSPADIFTLKDRV---------------------------------------------------------------------------------------------------KRGEITLVGRPGWKEKSVANLLDAIEERRNAIALHRFIFALGIRHVGEVTAKTLARHYGSLDAWLEGMKAAAREMPKPEWAELRTLKGLGPASVDALRAWFAAPENLETVRFHANAPDLRPETALRNL-GIKELKQPAITALVERYGTFAACLDGLRTAFAGAPGDAWTELVNVRDVGAIVAEEIASFFLEDR-NLEVIEGLRAAGVRVEDAEVR------------QAADSPVAGKTVVFTGTLTTMGRNEAKARAEALGAKVAGSVSKKTDYVVAGADAGSKAAKARELGVTVLSEEEWLALAG 793          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A0A8K3C2_9HYPH (DNA ligase n=3 Tax=Methyloceanibacter TaxID=1484898 RepID=A0A0A8K3C2_9HYPH)

HSP 1 Score: 414 bits (1065), Expect = 1.460e-125
Identity = 358/1083 (33.06%), Postives = 464/1083 (42.84%), Query Frame = 0

Query:  211 DRLRCRDVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELGVCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKE------EDKAVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAGIVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRI 1287
            D L  + V  L E EAA+EL  LA EIA HD  YY +D+P ++DA+YDALRLRN  IEA FP L+R +SP+ RVGAA                   PA         +G A                      V H   + SL NVFD++  REFVDR+RR + +                   +A VA                                                          L F  EPKIDGL+  L YE GRLV+AATRGDG  GE+VTAN L +GD  IP+ +                 +AR+                    FEVRGEI M + AF +LN E++      FA+ RNAAAGSLRQLD  VT  R L+FFAY                 W                  G++ +    TQ  +  A   WGF +  P    T S DE++  +R +   R+ L Y +DGVVYKLD L LQ+RLG  +R+PRWA+AHKF A++  T+L +I IQVGRTGALTPVA LEPV +GGV V  ATLHN  EI    V  GD V ++RAGDVIPQ+LG    + P              K F FPT CPACG+  V+E      ++ AV RC  GL CPAQ VE LKHFVS+ AFDI+GLG   +   Y+ G++++P DI  L A+ A    +                                                                                                   LE REGWG  SA+K+ DA+  A+ +VSL RFI++LG+  VG     ++A  +                  +  +A+  A    D +A    K + NI+G+  GP  A                         S +  F G                  +  N  V+  L  EV+         +   AVD    +A A V GKTVVFTG+L  M R E +A A  LGA+V GS+S+ TD VV G   G KL+ A++LGV+V++E EW + I
Sbjct:   17 DELAEKPVEELTEDEAASELARLAAEIAHHDALYYQKDDPEVSDADYDALRLRNEAIEARFPGLIREDSPAKRVGAA-------------------PA---------DGFAK---------------------VTHAVPMLSLGNVFDDDGVREFVDRIRRFLGL-------------------DADVA----------------------------------------------------------LAFTTEPKIDGLSIGLRYEGGRLVQAATRGDGYVGENVTANVLTIGD--IPKQV-----------------KARDFP----------------DPFEVRGEIYMSHSAFAALNAEQEKAGAKIFANPRNAAAGSLRQLDSSVTASRPLQFFAY----------------AW------------------GEAASLPADTQWGVYEAFAKWGFPL-NPLATLTDSLDEMLGIYREIEEGRAGLDYDIDGVVYKLDRLDLQQRLGFVSRSPRWAIAHKFPAQKATTILRDIEIQVGRTGALTPVAKLEPVTVGGVVVQNATLHNEDEIARKDVRIGDTVILQRAGDVIPQILGPVLDKRPKS-----------AKPFKFPTVCPACGSHAVREINPNTGKEDAVRRCTGGLICPAQRVERLKHFVSRNAFDIEGLGEKNIQAFYDEGLIQSPPDIFTLAARDAKAEEK---------------------------------------------------------------------------------------------------LESREGWGPTSAQKLFDAI-AARRNVSLDRFIYALGIRHVGEITGRLLARSYGT--------------IDNFLKAMGEAAKDRDGDAF---KDLDNIDGI--GPTAA-------------------------SAIADFFG------------------EPHNTEVIHDLLEEVK--------PKPLEAVD----NASA-VSGKTVVFTGTLEHMTRAEAKAQAERLGAKVAGSVSKNTDYVVAGPGAGSKLKNAKDLGVDVLTEGEWLKLI 717          

BLAST of mRNA_P-lacustris_contig979.16102.1 vs. uniprot
Match: A0A3A0EF87_9HYPH (DNA ligase n=1 Tax=Hyphomicrobiales bacterium TaxID=1909294 RepID=A0A3A0EF87_9HYPH)

HSP 1 Score: 414 bits (1064), Expect = 1.930e-125
Identity = 356/1088 (32.72%), Postives = 459/1088 (42.19%), Query Frame = 0

Query:  216 RDVAGLDEAEAAAELEALAEEIAAHDVRYYLEDEPSITDAEYDALRLRNGDIEAAFPFLVRSNSPSSRVGAATVRAETEATTNQQQKQPAAPAPVLTLKKLGEGGAASPTATAAVAPVAPSRAVRLPVVEHLRSLGSLDNVFDEEKAREFVDRVRRAVDVATLGEGGGAEPHVGGGARDNAPVAAGGEGVEAGVEGVADEGVGVAMAVNGISTKAPAAVRVGILGGGAPTETRVQQLPQQRELLFVAEPKIDGLTCALLYEDGRLVRAATRGDGTRGEDVTANALALGDAIIPRYLPLSSPAAAVSEGPGMPTRAREAEARAGATEGASSSAAPSGRFEVRGEILMPYEAFESLNEERKAEDLPAFASARNAAAGSLRQLDPDVTRRRGLRFFAYGAAVASSRGAGEAGVGVWAGGDEKMERQPGSEAEVVGDSLASAFGTQEALLRALEGWGFEVARPRLAPTASADELVAFHRRMVRDRSSLGYAVDGVVYKLDDLALQERLGASTRAPRWAVAHKFGAEEGETVLLEIVIQVGRTGALTPVAVLEPVVLGGVTVSRATLHNAREIEELG-----------VCPGDRVRVRRAGDVIPQVLGLAQGRPPDRDVDSEGDGDVGGKAFAFPTACPACGTAVVKEEDKAVTRCPAGLSCPAQVVEHLKHFVSKGAFDIQGLGPARLNELYEAG----IVRNPVDILRLRAKTAAQARRPAAADATGSTIVTAPTDEDDGEGKGAEDGLEKAFGRDGTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILEGREGWGRLSARKVLDAVDKAKDDVSLGRFIFSLGVPLVGARMADVIAEEFNQDFHAFWGALTRQSRASDASQALEAAQLAHDSEALEGTKAITNINGVASGPFVAASAXXXXXXXXXXXXXXXXXXXXXXSTLESFRGVQGMGPAILTSLLRFARADNGNRPVVEALASEVRFTARAQRGDRNRRAVDGGGESAGAWVEGKTVVFTGSLTRMKRTETQAAARELGARVTGSISRKTDLVVVGLNPGGKLEQARELGVEVISEEEWYRRIG 1288
            + V  L   EAA EL ALA EIAAHD RYY ED P+++DAEYDALRLRN +IEAAFP LVR++SPS+ VGAA                                    P+ T A              V H R + SLDNVF E   R+FV  VRR + +A                                                                               REL F AEPKIDGL+ +L YE+G LV AATRGDG  GE+VTAN   + D  IP+ LP  +P                                     EVRGE+ M  + F +LNE    E    FA+ RN AAGSLRQ DP++TR R LRFFAY                 W                  G+      GTQ   +R L  WGF V   R+A   S +E+++ +R +  +R+SL Y +DGVVYK+D L LQERLG  +R+PRWA AHKF AE+  T+L  I IQVGRTGALTPVA LEPV +GGV V+ ATLHN   I  +G           +  GD V V+RAGDVIPQVL +    P  R  DS          + FP  CPACG+  V+EE +AV RC  GL CPAQ VE ++HFVS+ AFDI+GLG  ++   +        +R P DI  L+ +  A   R                                                                                                   LE  +G+G++S RK+  A+++ +  V+L RF+F+LG+  VG   A  +A  F                       L  A+L   +EA            +  G     +A                         +   GV G+G  +  +L+ F   ++ N   ++AL  +V        G+              + V GKTVVFTG+LTRM R E +A A  LGARV+GS+S+KTDLVV G   G KL+QA  LG+EVI E+ W+  +G
Sbjct:    6 KPVEDLTPQEAAEELAALAAEIAAHDRRYYAEDAPTVSDAEYDALRLRNAEIEAAFPELVRADSPSTSVGAA------------------------------------PSGTFAQ-------------VRHARPMLSLDNVFSEADLRDFVASVRRFLALA-----------------------------------------------------------------------------DDRELSFTAEPKIDGLSMSLRYEEGELVTAATRGDGETGENVTANVRTIAD--IPQRLPADAPQV----------------------------------VEVRGEVYMRRDDFLALNERMAHEAGRTFANPRNFAAGSLRQKDPEITRSRPLRFFAY----------------AW------------------GEVSQPLAGTQLEAVRRLGSWGFPV-NDRMALCRSVEEMLSCYRAIEAERASLPYDIDGVVYKVDRLDLQERLGFRSRSPRWATAHKFPAEKATTLLRAIEIQVGRTGALTPVARLEPVTVGGVVVTNATLHNEDYIRGIGNDGNPIREGRDIRVGDTVLVQRAGDVIPQVLDIV---PEKRPADSI--------PYVFPDVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQIEFFFNHADPSLSIRTPADIFTLQERQQASLTR---------------------------------------------------------------------------------------------------LENVDGFGQVSVRKLYAAIEERRK-VALSRFLFALGIRHVGETNARRLARHF-----------------------LSFARLREVAEAAR----------MPQGKGDPGNAEW-----------------------QELVGVNGIGAVVAQALVDFFGEEH-NLAALDALLDKVTPLDEEPVGE------------VSSPVAGKTVVFTGALTRMSRDEAKAMAERLGARVSGSVSKKTDLVVAGPGAGSKLKQATGLGIEVIDEDAWFVLVG 716