prot_P-lacustris_contig961.15968.1 (polypeptide) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A6H5JWM1_9PHAE (RING-CH-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JWM1_9PHAE)

HSP 1 Score: 1181 bits (3055), Expect = 0.000e+0
Identity = 867/1539 (56.34%), Postives = 950/1539 (61.73%), Query Frame = 0

Query:   10 PQLFLQRLNTACIWSDCISGIIVTAAIILSFLSLMTFADFLADQLLRDDFDGIGLDGMNLVEAMAEAMDDLRFDNHNHNRNHDRALPPLQEAPPLEPPPRDGPDPDRRFNHGEGGARGA---NAAAPG-------ARLGARAAAPLAEEKRLLDNDPGGNGDFAEGEAFAETVHQRRAASLQRPASREDVADPGEGPGHEADGARSGSRRTGSGGSGGVIGSGGSSLTEVAATRGSSSAKEGGRMTMRELQEAVASAAEKRNMRH-QYPLDSDPMDIXXXXXXXXXXXXXXXXXEEVAPRDTFLGLEYRPPTPPRKVLVEDN-AESANGMLTPVAAAXXXXXXXATAGAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATAGADGGGAAARPTLGGGEHHALGRTAYHPGETRGSGLSRGAAAAAAERGEVGAGATPALPT---------PMPTREGVSDGAG----ATAVREGADAAAPKGREN-------------TERYNGDGFEPPPRPPTPPRGDAEPAAGPGVRPEPRQAPGPXXXXXXXXAQGDDVDXXXXXDFLDGDMDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHVIASLGGRSE-YLALKDIVQGWYVLATSSEEQ-NTLRLPDLATICLGYMVMSGMVFMWRGIINAISDRTNTQILLRAKKAMRVISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVGALFPAVMPIKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLPQPIVARQPRPAPT-----------ADGVATSSAPGGSVAAAVATAGYATSSLRRRSTPARDGGVSSVSGMGSTGDAGEEGDKEAFLVGDDXXXXXXXXXXXXXXXXXXXXXXXDAEEGIVVGPPLRRPPP-GWDDPAGVAQGRWAWGAEPPGEVQLNLAPRRRHGARAVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLFALVHLPRRWTHDTASFAVGLTILKVVTPSLYRGWCLARVKLLAWRRGVAAPPGPREAVAIAARAADVVARIEATLANIMAEE-GKDGD-----------QHA----------LGMQLLVERQTRLNEDMRQQLESLREEEVSAV----------------------------------------------AAAATTRTA---------------------FTRATATLLAGAALWLVAAPLLAAGLFETAFVVTAHAWAEKGPFGALPCWRQPLRSLVLGMFLLHAWVYACRAEVPRWLVAMRRALGIGARGRFGGLRGGGVGXXXXXXXXXXXXXVEGQEGKQLRWRECIEAALLQVDAALVRGSWAEVSPRVILWNFAGAVVLRLSAMVAIPALVSAAAGAG-AACSADEQFVQL 1406
            PQLFLQRLN ACIWSDCISGIIVTAAIILSFLSLMTFADFLADQLLRDDF GIGLDGMNLVEAMAEAMDDLR DN+N+N+ HD+  PPLQE   L P P  G  PDRR N G  G R     +A AP        A  GARAA P     R  +    G G+  +GEA A    +R AA  +  +     A P +G   +AD  R     +  GG        G S  E  AT   +  ++G R+++ E+ E+ A+AAE+RN    ++  + DPM                   EE  P  TFLGL+YR PTPPRKV +ED+ A S+  + +  A  XXXXXXX  AGA                                                        GE H+LGR     G     G SRG AAA                          P    EG          A A+R GA+ A P+ R +                         P        +AEPA   G        P P         + +D +     +FLDGDMDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHV+ASLGGRSE Y AL+D++QGWY LATSSEEQ NTLRLPDLATICLGYMVMS MVFMWRGIINAIS+RTNTQILLRAKKAMRVI+AVVKVGLLLSLKMLLLPLLLG CLD TTLPLFG TGEAR RFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHP+VLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPT+LVG LFPAVMP++LELRYGMPQLQIP ELVILHLAMLAFLEKYQ NRIGEMQHTWLVAVCGRLGLTRFVLPQPIVAR PRP P            AD             A  ATA    S+LRRRS  +R        G+G++G                XXXXXXXXXXXXXXXXXXXXXXX                  GWDDPAGVAQGRWAWGAEPPGEVQLNLAPRRRHGARAVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLF+L+HLPRRWTHDTASFAVGLT+LK VTP+L+R WC AR+KLLA RRG A PP PRE+  IAA AA++ AR EA LA +MAE  G DG            QH           L     +ERQTR  +++RQQL  +REE V+A                                               A A     A                      +RA ATLLAGA LWLV APLLAAGLFETAFVVTA AWAEKGP GALPC  QPLRSL LGMFLLHAW YACRA VPRW+ A RRALGI  R   GG          XXXXXXXX    G E + L W E +EAALLQV+A L  GSWAEVSP  +LWNFAGAVV+RL AMV IP +VS  AGA   A  A  QFVQ+
Sbjct:  186 PQLFLQRLNMACIWSDCISGIIVTAAIILSFLSLMTFADFLADQLLRDDFGGIGLDGMNLVEAMAEAMDDLRIDNNNNNQVHDQLPPPLQEPQQLGPLP--GHAPDRRANQGHRGGRVGGVGDAVAPXXXXXXXXAGRGARAAEPPTGGGRPNNR---GVGEAHQGEALAAIGERRSAAPREAESGGASPAPPADGSARDADHDRRIGNESTVGGXXXXXAHRGVSA-EGLATEEINLPRDGERVSVPEVAESAAAAAEQRNFAQPRFRRNVDPM--------AYDTDDAEGEEEEAGPGGTFLGLKYRAPTPPRKVRIEDDDATSSKDVASASAXXXXXXXXXXXAGARITARTTREEAK--------------------------------------------GEQHSLGRV---DGAATRVGFSRGPAAAXXXXXXXXXXXXXXXXXXXXXXXXXMPKIDEEGXXXXXXXXXDAAALRAGANNAEPRVRADGGGXXXXXXXXXXXXXXXXXXXXXXPE-------NAEPAVA-GAXXXXXXXPVPRVPLAAD--EEEDPEDDLNDEFLDGDMDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHVLASLGGRSELYPALRDVLQGWYELATSSEEQENTLRLPDLATICLGYMVMSSMVFMWRGIINAISNRTNTQILLRAKKAMRVIAAVVKVGLLLSLKMLLLPLLLGGCLDATTLPLFGCTGEARVRFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPDVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTRLVGTLFPAVMPVQLELRYGMPQLQIPVELVILHLAMLAFLEKYQ-NRIGEMQHTWLVAVCGRLGLTRFVLPQPIVARLPRPGPPPTTFGFSTFDDADXXXXXXXXXXXXEANGATAAPG-STLRRRSVSSR--------GLGASGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGWDDPAGVAQGRWAWGAEPPGEVQLNLAPRRRHGARAVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLFSLIHLPRRWTHDTASFAVGLTLLKAVTPTLHRAWCAARLKLLARRRGPATPPAPRESAVIAASAAELAARAEANLAGLMAERAGADGGGRXXXXXXXXXQHRELELRLQRDQLQQARQLERQTRRMKELRQQLNRVREEAVAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYVNDIAYRKAVAVLLAEAEEVTXXXXXXXXXXXXXXXXXXSRAAATLLAGAVLWLVVAPLLAAGLFETAFVVTAQAWAEKGPLGALPCCSQPLRSLALGMFLLHAWAYACRARVPRWIAATRRALGIAPRAGGGG---------RXXXXXXXXGDDGGLEERPLFWCERVEAALLQVEAGLAHGSWAEVSPHGVLWNFAGAVVVRLLAMVLIPTVVSLVAGAWMGADGAYGQFVQV 1634          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: D8LBR1_ECTSI (RING-CH-type domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBR1_ECTSI)

HSP 1 Score: 960 bits (2482), Expect = 0.000e+0
Identity = 743/1478 (50.27%), Postives = 796/1478 (53.86%), Query Frame = 0

Query:   10 PQLFLQRLNTACIWSDCISGIIVTAAIILSFLSLMTFADFLADQLLRDDFDGIGLDGMNLVEAMAEAMDDLRFDNHNHNRNHDRALPPLQEAPPLEPPPRDGPDPDRRFNHGEGGARGANAAAPGARLGA--------RAAAPLAEEKRLLDNDPGGNGDFAEGEAFAETVHQRRAASLQRPASREDVADPGEGPGHEADG----ARSGSRRTGSGGSGGVIG---SGGSSLTEVAATRGSSSAKEGGRMTMRELQEAVASAAEKRNMRHQYPLDSDPMDIXXXXXXXXXXXXXXXXXEEVAPRDTFLGLEYRPPTPPRKVLVEDNAESANGMLTPVAAAXXXXXXXATAGAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATAGADGGGAAARPTLGGGEHHALGRTAYHPGETRGSGLSRGAAAAAAERGEVGAGATPALPTPMPTREGVSDGAGATAVREGADAAAPKGRENTERYNGDGFEPPPRPPTPPRGDAEPAAGPGVRPEPRQAPGPXXXXXXXXAQGDDVDXXXXXDFLDGDMDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHVIASLGGRSE-YLALKDIVQGWYVLATSSEEQ-NTLRLPDLATICLGYMVMSGMVFMWRGIINAISDRTNTQILLRAKKAMRVISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVGALFPAVMPIKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLPQPIVARQPRPAPT-----------ADGVATSSAPGGSVAAAVATAGYATSSLRRRSTPARDGGVSSVSGMGSTGDAGEEGDKEAFLVGDDXXXXXXXXXXXXXXXXXXXXXXXDAEEGIVVGPPLRRPPPGWDDPAGVAQGRWAWGAEPPGEVQLNLAPRRRHGARAVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLFALVHLPRRWTHDTASFAVGLTILKVVTPSLYRGWCLARVKLLAWRRGVAAPPGPREAVAIAARAADVVARIEATLANIMAEEGKDGDQHALGMQLLVERQTRLNEDMRQQLESLREEEVSAVAAAATTRTAFTRATATLLAGAALWLVAAPLLAAGLFETAFVVTAHAWAEKGPFGALPCWRQPLRSLVLGMFLLHAWVYACRAEVPRWLVAMRRALGIGARGRFGGLRGGGVGXXXXXXXXXXXXXVEGQEGKQLRWRECIEAALLQVDAALVRGSWAEVSPRVILWNFAGAVVLRLSAMVAIPALVSAAAGAGA-ACSADEQFVQLGHFRAALLALFLTRVSAYLVKPLRAWFKTLHDSVRDDRYLVGMRLQDYSDQT 1458
            PQLFLQRLN ACIWSDCISGIIVTAAIILSFLSLMTFADFLADQLLRDDF GIGLDGMNLVEAMAEAMDDLR DN N+N+ HDR  PP QE   L P P  G  PDRR NHG GG                       RAA P A   R    D  G G+  +GEA A       A   +RPA+  +    G  P H ADG    A    RR GS  +G       + G    E  AT   +  +EG R+++ EL E+ A+A  +   + ++  + DPM                   EE AP DTFLGL+YRPPTPPRK   +  A  +                                     XXXXXXXXXXXXXX             ADGGG                                              G  P +P                        AA +  E  +  N                                                        +FLDGDMDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHV++SLGGRSE YLAL+D+VQGWY LATSSEEQ NTLRLPDLATICLGYMVMS MVFMWRGIINAIS+RTNTQILLRAKKAMRVI+AVVKVGLLLSLKMLLLPLLLGVCLD TTLPLFG TGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPT+LVG LFPAVMP++LELRYGMPQLQIP ELVILHLAMLAFLEKYQ NRIGEMQH+WLVAVCGRLGLTRFVLPQPIVAR PRP P            ADG            A  ATA    S+LRRRST +R  G S                         XXXXXXXXXXXXXXXXXXXXXXX                 GWDDPAGVAQGRWAWGAEPPGEVQLNLAPRRRHGARAVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLF+L+HLPRRWTHDTASFAVGLT+LK                                                                                                                                                                                                                                               AALLQV+A L  GSWAEVSPR +LWNFAGAVV+RL AMV IP +VS  AG    A  AD QFVQLGHFRAAL AL LTR SAYL++P+RAW   LHDSVRDDRYLVGMRLQD+SD++
Sbjct:  186 PQLFLQRLNMACIWSDCISGIIVTAAIILSFLSLMTFADFLADQLLRDDFGGIGLDGMNLVEAMAEAMDDLRIDN-NNNQVHDRVPPPPQEPQQLGPLP--GHAPDRRANHGHGGXXXXXXXXXXXXXXXXXXXXXXXRAAEPPAGGGRP---DNRGVGEAHQGEALA-------AIEERRPAAPREAESGGGSPAHPADGSARDADDHDRRIGSNSTGXXXXXXXAHGGVKAEGLATEERNIPREGERVSVPELAESAAAAERQNLAQPRFRRNVDPM--------AYDTDDEEGEEEEAAPGDTFLGLKYRPPTPPRKGGGQGRAAQSR---------------------------TRRWSGHKDXXXXXXXXXXXXXXLRAGANNAEPRVRADGGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------XXXXXXVGQVPRVPL-----------------------AADEEEEPEDDLN-------------------------------------------------------DEFLDGDMDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHVLSSLGGRSELYLALRDVVQGWYELATSSEEQENTLRLPDLATICLGYMVMSSMVFMWRGIINAISNRTNTQILLRAKKAMRVIAAVVKVGLLLSLKMLLLPLLLGVCLDATTLPLFGCTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTRLVGTLFPAVMPVQLELRYGMPQLQIPVELVILHLAMLAFLEKYQ-NRIGEMQHSWLVAVCGRLGLTRFVLPQPIVARLPRPGPPPTTFGFSTFDDADGXXXXXXXXXXXXANGATAAPG-STLRRRSTSSRGLGASXXXXXXXXX-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGWDDPAGVAQGRWAWGAEPPGEVQLNLAPRRRHGARAVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLFSLIHLPRRWTHDTASFAVGLTLLK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AALLQVEAGLAHGSWAEVSPRGVLWNFAGAVVVRLLAMVLIPTVVSLVAGTWMDAYGADGQFVQLGHFRAALFALTLTRASAYLIQPVRAWLTALHDSVRDDRYLVGMRLQDHSDKS 1283          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A7S2RW40_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RW40_9STRA)

HSP 1 Score: 438 bits (1127), Expect = 9.470e-128
Identity = 285/594 (47.98%), Postives = 365/594 (61.45%), Query Frame = 0

Query:  546 MDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLD-PHVIASLGGRSEYLALKDIVQGWYVLATSSEEQNTLRLPDLATICLGYMVMSGMVFMWRGIINAISDRTNTQILLRAKKAMRVISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVGALFPAVMPIKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLPQPIVARQPRPAPTADGVATSSAPGGSVAAAVATAGYATSSLRRRSTPARDGGVSSVSGMGSTGDAGEEGDKEAFLVGDDXXXXXXXXXXXXXXXXXXXXXXXDAEEGIVVGPPLRRPPPGWDDPAGVAQGRWAWGAEPPGEVQLNLAPRRRHGARAVPLVAVLLLAAWATVLVAALCLVVL----PFLLGRLLFALVHLPRRWTHDTASFAVGLTILKVVTP---SLYRGWCLARVKLLAWRRGVAAPP 1131
            +++H+A+DELLGIRGP+T+LLRN+LWLLAF+ AYLGLFAF PFSIG+S+  +V + +D P ++A+L    +   L+ +V  +    T+++E +TL+LPDL TI LGY+VMS M+FMWR  ++A S RT+ Q++ R +  +   +AVVKVG+LL LKMLLLPLLLGVCLD  TL LF ++   R  F AQ+LVGSLLLHWVLGITFMLFVTVSVLQLREV+HP++ + IIRPQEPHPDLLGSLLQESG THARRM MSLAIY+ L+ +FV +PT +V    P  +P++L L Y  PQLQ+P ELVI HL++L FLE+++ NRIGE+QH WLV V G LGLTR++LP P +   P  AP         APGG                                   + G+AG            D                             +VG PL+RPPPGWDD AG  QGRWAWG E P EV+ NLA RR      + L  VLL   WATVL    CLV+L    P +LGR +F L+ +P    HD  +FA+G+ +     P   ++YR   L R  L  W   +A  P
Sbjct:  459 VELHVALDELLGIRGPITVLLRNILWLLAFNCAYLGLFAFIPFSIGSSILASVRRNVDSPVLLAALELVPQ--GLRQVVDDF--ATTAAKEDHTLQLPDLGTIALGYLVMSCMIFMWRAAVSAASQRTSLQLMSRVRLILDCTAAVVKVGILLFLKMLLLPLLLGVCLDAATLRLFTASAAERVHFTAQNLVGSLLLHWVLGITFMLFVTVSVLQLREVLHPDIFAAIIRPQEPHPDLLGSLLQESGATHARRMIMSLAIYVFLLFVFVSLPTSMVLTWAPTAVPLELNLAYIAPQLQVPVELVIFHLSVLGFLERFK-NRIGELQHIWLVRVSGALGLTRYLLPLPRLEAGPEGAPD-----PLPAPGGQ----------------------------------AVGEAGXXXXXXXXXXXXDE----------------------------LVGAPLQRPPPGWDDLAGGGQGRWAWGNEEPTEVERNLAKRRTPDRLTLRL-GVLLGLGWATVL----CLVLLGTWAPLVLGRRVFQLLRIPTSVIHDPFAFALGIGLCLAARPLVAAVYRH--LTRGSLETWCHTMARRP 973          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A835YNH4_9STRA (RING-CH-type domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNH4_9STRA)

HSP 1 Score: 377 bits (968), Expect = 3.780e-104
Identity = 278/691 (40.23%), Postives = 345/691 (49.93%), Query Frame = 0

Query:  549 HMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLD-------PHVIASLGGRSEYLALKDIVQGWYVL--------------ATSSEEQNTLRLPDLATICLGYMVMSGMVFMWRGIINAIS-DRTNTQILL--RAKKAMRVISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVG--------------------------------------------ALFPAVMPIK----------------LELRYGMPQLQIPAELVILHLAMLAFLEKYQQ---------------NRIGEMQHTWLVAVCGRLGLTRFVLPQPIVAR--QPRPAPTADGVATSSAPGGSVAAAVATAGYATSSLRRRSTPARDGGVSSVSGMGSTGDAGEEGDKEAFLVGDDXXXXXXXXXXXXXXXXXXXXXXXDAEEGIVVGPPLRRPPPGWDDPAGVAQ-----------------------------------------GRWAWGAEPPGEVQLNLAPRRRHGAR--AVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLFALVHLPRRWTHDTASF 1095
            H+A DELLG+RGP+T+LLRN LWLLAF+GAYLGLFAF P+S+GASV  A++KYLD                  ++ +LK +++                   + S+ E +TL+L DL TICLGY            ++ A   DR    +L   RA++AMRV++AVVKVGLLLSLKMLLLPLLLG CLD   LP+F ++   R  F+  +L GSLLLHWV+GITFMLFVTVSVLQLREVVHP+VL+ +IRPQEPHPDLLGSLLQESGRTHARRMAMSLAIY+AL+L+FVWVP+++                                              A  P V P                  L L+Y +PQLQ+P ELV+ HLAMLAFLE+Y+                + IG  QH WLV  C RLGLTR++LP PI+AR    RPA   + +      GG               L     P  D  ++  SG+  +                                        D ++GIVVG PL RPPPGWDDPAG  Q                                         GRWAWG E  G V+ +LAPRR    R    P +A LL  AW   L      V +PF LGR  FA + +P  W+HD  +F
Sbjct:  600 HVAFDELLGLRGPLTVLLRNALWLLAFNGAYLGLFAFIPYSVGASVAAALAKYLDGAQALPDQXXXXXXXXXXQFQSLKGMLRATVAALGETAAAAGTPPQASASAAEDHTLQLADLLTICLGYAAEVCTPRAAARVLRACKRDRGRVAVLTLQRARRAMRVLAAVVKVGLLLSLKMLLLPLLLGACLDAAALPIFAASPADRLHFLRTNLAGSLLLHWVMGITFMLFVTVSVLQLREVVHPDVLAGVIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYVALLLLFVWVPSRIAARXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAALPGVPPAXXXXXXXXXXXXXXXXXLRLQYLVPQLQVPMELVVFHLAMLAFLERYKXXXXXXXXXXXXXXXXDSIGRTQHEWLVRWCQRLGLTRYLLPLPILARTLDGRPAGPENPLVRLPVAGGRP------------DLPYDVVPGGDAPIALDSGLRGS----------------------------------------DEDDGIVVGAPLVRPPPGWDDPAGAHQVMSAAAFEVPKGLDFTILLYGTAGFTIFGTAXXXXXXXXXXGRWAWGGEYKGPVERSLAPRRSPQGRLGVAPPMAALLAVAWVHTLARCATTVCVPFALGRAAFAALRVPLAWSHDPFAF 1238          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A7S2CYG7_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2CYG7_9STRA)

HSP 1 Score: 357 bits (916), Expect = 8.040e-101
Identity = 195/346 (56.36%), Postives = 265/346 (76.59%), Query Frame = 0

Query:  546 MDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHVIASLGGRSEYLALKDIVQGWY-VLATSSEEQNTLRLPDLATICLGYMVMSGMVFMWRGIINAISDRTNTQILLRAKKAMRVISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVGALFPA-VMPIKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLP 889
            M++H+A+DEL+GIRGPVT+LLRN+LWLLAF+ AYLGLFAF PFSIG+SV   +  Y++  V+ +L   + YL  + +V+    + +T+ EE +TL+LPDL ++ LGY+VMS M+F WR I++A+   T   ++ R + A+   +AVVKVG+LL LKMLLLPLLLGVCLD +TL +FG++ + R RF AQ+LVGSLLLHWVLGITFMLFVTVSVLQLREV+HP++L+ +IRPQ+PH      LL+ESG THARRM MSLAIY+ L+ + V +PT +  A  P  ++P++L+  Y +PQ Q+P ELV+ HL++LAFLE+++ N+IGE+QH WLV  C  LGLT F+LP
Sbjct:  279 MELHVAVDELVGIRGPVTILLRNMLWLLAFNCAYLGLFAFIPFSIGSSVLSTLGSYVNSPVMVAL---NNYLVPQTLVKVLKEISSTAQEEDHTLQLPDLFSMALGYLVMSLMIFTWRSIVSAVCQGTPMPLIGRIRLALDCTAAVVKVGILLFLKMLLLPLLLGVCLDASTLMVFGTSAKDRIRFTAQNLVGSLLLHWVLGITFMLFVTVSVLQLREVMHPDILASVIRPQDPHXXXXXXLLRESGGTHARRMVMSLAIYIMLLFLLVHLPTLVFQACAPPHILPVQLQFSYLLPQFQVPLELVMFHLSVLAFLERFK-NKIGELQHAWLVRACRFLGLTHFLLP 620          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: W7U493_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7U493_9STRA)

HSP 1 Score: 322 bits (826), Expect = 3.160e-90
Identity = 195/358 (54.47%), Postives = 251/358 (70.11%), Query Frame = 0

Query:  542 LDGDMDV--HMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLD-PHVIASLGGRSEYLALKDIVQ-GWYVLATSSEEQNTLRLPDLATICLGYMVMSGMVFMWRGIINAISDRTNTQILLRAKKAMRVI---SAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVG-ALFPAVMP--IKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLP 889
            +D DMDV  H+A+DEL+G+RGP   L+RN+LWL+AF+GAYLGLFAF P+S+GA+V  A  KY++ P  I +L     +L  + I +    V   ++ E++TL+LPDL  I LGY++MS MVF WR I+ A+  R     L    K +R++   +AVVKVG+LL  KM L PLLLG+ LD  T  + G     R RF  ++LVG+LLLHWV GITFMLFVTVSVLQLREV+HP++L+ IIRPQEP PDLLGSLLQESG THARRMA+SL IY+ L+L+FV++P +L+  A F A       L   Y  PQLQIP EL+I HLAMLAFLE + +N IG +Q+ WL   C  LGLTR++LP
Sbjct:   82 IDADMDVELHVAVDELMGVRGPFVYLVRNILWLVAFNGAYLGLFAFIPYSVGAAVVGAAEKYVETPLGICTL----RFLVPRSICKLAAEVCQAAAAEEHTLQLPDLGVILLGYLLMSAMVFTWRTIVCAVCKRMAVTALPMLGKVVRMLECTAAVVKVGVLLFSKMALCPLLLGLWLDAATTQVIGPDHAVRVRFATENLVGALLLHWVAGITFMLFVTVSVLQLREVLHPDILARIIRPQEPQPDLLGSLLQESGLTHARRMAISLGIYVLLLLLFVYLPAELLAFAGFNATGNGLFHLNFFYLAPQLQIPFELIIFHLAMLAFLE-HTKNAIGRLQYMWLRPFCKALGLTRYLLP 434          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A4D9D1W8_9STRA (RING-CH-type domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D1W8_9STRA)

HSP 1 Score: 318 bits (815), Expect = 4.620e-87
Identity = 189/352 (53.69%), Postives = 248/352 (70.45%), Query Frame = 0

Query:  546 MDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLD-PHVIASLGGRSEYLALKDIVQ-GWYVLATSSEEQNTLRLPDLATICLGYMVMSGMVFMWRGIINAISDRTNTQILLRAKKAMRVI---SAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVG-ALFPAVMP--IKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLP 889
            +++H+A+DEL+G+RGP   L+RN+LWL+AF+GAYLGLFAF P+S+GA+V  A  KY++ P  I +L     +L  + I +    V   ++ E++TL+LPDL  I LGY++MS MVF WR I+ A+  R     L    K +R++   +AVVKVG+LL  KM L PLLLG+ LD  T  + G     R RF  ++LVG+LLLHWV GITFMLFVTVSVLQLREV+HP++L+ IIRPQEP PDLLGSLLQESG THARRMA+SL IY+ L+L+FV++P +L+  A F A       +   Y  PQLQIP EL+I HLAMLAFLE + +N IG +Q+ WL   C  LGLTR++LP
Sbjct:  301 VELHVAVDELMGVRGPFVYLVRNILWLVAFNGAYLGLFAFIPYSVGAAVVGAAEKYVETPLGICTL----RFLVPRSICKLAAEVCQAAAAEEHTLQLPDLGVILLGYLLMSAMVFTWRSIVCAVCKRMAVTALPMLGKVVRMLECTAAVVKVGVLLFSKMALCPLLLGLWLDAATTQVIGPDHAVRVRFATENLVGALLLHWVAGITFMLFVTVSVLQLREVLHPDILARIIRPQEPQPDLLGSLLQESGLTHARRMAISLGIYVLLLLLFVYLPAELLAFAGFNATGTGLFHINFFYLAPQLQIPFELIIFHLAMLAFLE-HTKNAIGRLQYMWLRPFCKVLGLTRYLLP 647          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A7S1FWY8_9STRA (Hypothetical protein n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1FWY8_9STRA)

HSP 1 Score: 293 bits (750), Expect = 7.630e-78
Identity = 236/619 (38.13%), Postives = 323/619 (52.18%), Query Frame = 0

Query:  546 MDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHVIASLGGRSEYLALK-DIVQGWYVLATSSEEQNT-LRLPDLATICLGYMVMSGMVFMWRGII------------NAISDRTNTQ---ILLRAKKAMRVISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLV----GALFPAVMP-IKLELRYGMP-QLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLPQPI-----VARQ------------PRPAPTADGV-----ATSSAPGGSVAAAVATAGYATSSLRRRSTPARDGGVSSVSGMGSTGDAGEEGDKEAFLVGDDXXXXXXXXXXXXXXXXXXXXXXXDAEEGIV------VGPPLRRPPPGWDDPAG---VAQGRWAWGAEPPGEVQLNLAPRR---RHG-----ARAVPLVAVLLLAAWATVLVAALCLVVLPFLLGRLLFALVHLPRRWTHDTASFAVGLTIL 1102
            +++H+A+DELLG+RGP   + RNV W++  +  YLGLF F P        H + +   P V  ++  R +   L  ++ + W V   +++  ++ L+LPDLATI  GY  M   +F+   I+            N + +  N     + ++        +A+ KV  LL LKMLLLPL+LG  LD+TTLPLF  T + R +F   +L G+LLLHWV GITFML VTVSVLQLREV+HP++L+ +IRPQEP PDLLG+LLQ+   THA+RM +SLAIY+AL+ +FVWVP  LV    G+ F   +P ++L+  Y +P QLQ+P EL + HL MLAFLEKY+ N IGEMQH WLV +CG+LG+   VLP  +     VA +            P  +P  D        T+SAP   +   +     A  S R R  P +     S+ G+    DA E          D                        D +  ++       G P+ RPP GWDD  G    AQGRWAWG E   EV+   A R    R G     A+ +  +AVL    WA  LV  +  + LP + GR L     +P  W HD  +F +GL  L
Sbjct:  307 VEIHLALDELLGVRGPPGAMFRNVAWVVLINTTYLGLFVFVP--------HEIGRIFWPAVRQTVAARIDLQQLPHNLTRTWDVYEKAAQHHDSILQLPDLATILAGYFTMGVSIFVCHAILAYAVKKRKQATDNTLDEDENDTRWWVAMKMAGVAECFAAIFKVATLLFLKMLLLPLVLGAWLDITTLPLFQHTLDHRLQFAGCNLFGALLLHWVAGITFMLLVTVSVLQLREVLHPDLLAQMIRPQEPQPDLLGNLLQDRVATHAKRMTLSLAIYIALLGVFVWVPAALVHRVGGSHF---LPFLRLQFCYLLPHQLQVPVELFMFHLCMLAFLEKYK-NHIGEMQHYWLVYICGKLGIVESVLPLQVQEFELVAERNLFLNAPCNDTPPNTSPPTDPFWEDFSHTASAP---LLDTLLRDYAAHPSRRHRFQPGK-----SLQGLRVLHDAPEPFQLYLDAPPDFPSPFPPLPSCLGRYRLRIHGDLGDPDPPVLQFWRETTGRPILRPPAGWDDLMGGGAEAQGRWAWGRERRSEVERGTAHRTPLWRGGGPGAAAKLLGKLAVLTATWWAFHLVLIVAALSLPLVAGRHLMTAARVPEGWVHDPLAFPLGLAAL 905          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A7S4W2J4_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4W2J4_9STRA)

HSP 1 Score: 284 bits (727), Expect = 3.770e-74
Identity = 189/418 (45.22%), Postives = 253/418 (60.53%), Query Frame = 0

Query:  545 DMDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKYLDPHVIASLGGRSEYLAL---KDIVQGWYVLATSSEEQNT-LRLPDLATICLGYMVMSGMVFMWRGIIN----------------AISDRT-------------------------------------------NTQILLRAKKAMRV--ISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVGAL-----FPAVMPIKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLPQPI 892
            DM++H+A+DELLG+RGP+  L+RN+LWLLAF+  YLGLFAF P +IG SV    S   +  V++S+  R   L +   + +V     + T S+  +T L+LPDLATI LGY+ M+ MVF W+G ++                A  DR                                            + Q+ +  + A+ +   +A+VKVG+LL LKMLLLPLLLG+ LD +TL LFGS+   R  +    L GS+LLHWV GITFML VTVSVLQLREV HP++L+ +IRPQEP PDLLG+LLQESG THA+RM +SL IY AL+L+ VW+P +L+ AL      P   P K    + +PQLQ+P EL++ HL+MLAFLEKY+ N IGEMQH WLV +C  + LT  +LP+ +
Sbjct:  487 DMEIHVALDELLGLRGPLFALVRNLLWLLAFNTTYLGLFAFIPHTIGESVYSTAS---NSTVLSSVISRLPLLPIDGERTLVSIINDINTESKRLHTSLQLPDLATIALGYLSMAFMVFFWKGALSLYKQFGKNADNDGAGEAAQDRAVNREERLDMHHGRRNLFQDVDGDQDDFADDEFELEDLQNDPPIDVQVAISQRLALVLDCAAAIVKVGVLLFLKMLLLPLLLGIWLDASTLSLFGSSLNERILYAGSDLFGSILLHWVAGITFMLIVTVSVLQLREVAHPDLLARVIRPQEPQPDLLGNLLQESGATHAKRMLLSLGIYAALLLIHVWLPARLLVALGIGRHLPFFRP-KFWFLF-LPQLQVPVELLVFHLSMLAFLEKYK-NHIGEMQHYWLVFMCKWMCLTDHILPREV 898          

BLAST of mRNA_P-lacustris_contig961.15968.1 vs. uniprot
Match: A0A1V9ZFK7_9STRA (E3 ubiquitin-protein ligase MARCH6 (Membrane-associated RING finger protein 6) n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9ZFK7_9STRA)

HSP 1 Score: 280 bits (717), Expect = 4.110e-74
Identity = 156/354 (44.07%), Postives = 239/354 (67.51%), Query Frame = 0

Query:  546 MDVHMAIDELLGIRGPVTMLLRNVLWLLAFHGAYLGLFAFFPFSIGASVTHAVSKY------LDPHVIASLGGRSEYLALKDIVQGWYVLATSSEEQNT---LRLPDLATICLGYMVMSGMVFMWRGIINAISDRTNTQILLRAKKAMRVISAVVKVGLLLSLKMLLLPLLLGVCLDVTTLPLFGSTGEARARFMAQHLVGSLLLHWVLGITFMLFVTVSVLQLREVVHPEVLSLIIRPQEPHPDLLGSLLQESGRTHARRMAMSLAIYLALVLMFVWVPTKLVGALFPAVMPIKLELRYGMPQLQIPAELVILHLAMLAFLEKYQQNRIGEMQHTWLVAVCGRLGLTRFVLPQ 890
            M++++A+DEL+G RGP+ +L+RNV W LAF+GAYLG+FAF P+++G+++   ++K+      LDP V+   G  ++  AL  +V    VL T+   +     L+L DL T  LGY    G + +WR ++N +S   +  +L      +  ++AVVKV  LL LKM++LP+LLG+ +D  TL LF ++   R  +   +++ +LL+HWVLGITFMLFVTV+VLQ+REV+HP++L+  IRPQEP+PD+L SLL E    HARRM +SLAIY AL+L+ V+ P ++V A+ P++ P+ L+  +    LQ+P ELV+ H+ +LA LE Y+ N IG  Q+ W++ +C +LGLT ++LP+
Sbjct:  361 MEINIAMDELVGFRGPLYLLVRNVSWFLAFNGAYLGIFAFIPYTLGSTLVSTLTKFSSAIPPLDPSVLE--GPIADMPALYRVVG--MVLNTTEAARTAGDCLQLVDLCTCALGYFAFCGTIVLWRALVNTVSSYNHRPLLAGLLWFLSCLNAVVKVSTLLLLKMIVLPILLGIGMDFATLQLFLASPWERLAYCMDNMLATLLVHWVLGITFMLFVTVAVLQMREVLHPDILAATIRPQEPNPDILKSLLSEKSIKHARRMVLSLAIYAALLLLLVYTPVRMVHAVAPSLFPLTLQFYHVFAPLQVPLELVLAHMTILAVLETYK-NEIGHFQYRWMLRMCAKLGLTEYLLPR 709