prot_P-lacustris_contig938.15788.1 (polypeptide) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: A0A6H5J7N3_9PHAE (E1 ubiquitin-activating enzyme n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5J7N3_9PHAE)

HSP 1 Score: 1647 bits (4264), Expect = 0.000e+0
Identity = 820/924 (88.74%), Postives = 859/924 (92.97%), Query Frame = 0

Query:    1 MPPDPAEGEDAA-----GSKVVVDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQGMTELEGREFTITEKGPFSFEIDCDTSSCGTFVSGYVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRADKGSLPEAGDMEHAEAVYGFAKACNEEGDFKAEGLEESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLSKEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGA-GQLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPPLR 918
            MPPDPAEG  A      GSK  VDESLYSRQLYVMGHEAQRRMA SNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYL+EADLG PRAAACVS+LAELNRYVGVS  TGEVTEA+I S+Q VVMIDA LDEQLRVND+CH KGVC+ISCD RGVFAYAFCDFGE+FVVSDTDGNQA+SCVVSS+TKDA+GLVTVMDDQRHNLVTGDVV F+S+QGMTELEGREFTITEKGPFSFEIDCDTSS GTFVSGYVNQ    VK+PSTLSF PLREAL KPEPFMETDFAKIGRPG+LHQAFRGL+++RADKGSLPEAGDME AEAV+  AK CNE+GDFKAEGL++SKDVILRLSLGARG+LNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDA EALP+KPL+KEEVQP+GCRYDGSIMVFGK MQ LLGKQKLFLVGAGAIGCEMLKNWAMMGVGCD  GQVHVTDMD+IEKSNLSRQFLFRESDIGRAKSLTAA AV+AMNPSLNIKPYEAKC+QETEELFSDDFYS LSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYL DPN+ +FLNSQHNTKL+TLTRISESLDS RPS F DCI WARLQFQ RFHNEIAQLLHNFPVDS+TSSGNPFWSGAKRPPCPLDF+  DALHMGFVKGAAVLRA MYGIKPPTD++VY  TLS MEVP FKPVDGVKIATTEAEAKEQ TG      QLEDVD QCERML ELPKP DMKDFRL+VVEFDKDLD+HMEFV AASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLV ME+YKIMQEKPLESYKNWFLNLALPQFSCSEPLPP +
Sbjct:   69 MPPDPAEGGKAVEAGGGGSKAGVDESLYSRQLYVMGHEAQRRMATSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLSEADLGNPRAAACVSRLAELNRYVGVSTTTGEVTEAMIGSYQVVVMIDAPLDEQLRVNDICHAKGVCFISCDARGVFAYAFCDFGESFVVSDTDGNQAASCVVSSVTKDAVGLVTVMDDQRHNLVTGDVVTFNSIQGMTELEGREFTITEKGPFSFEIDCDTSSLGTFVSGYVNQ----VKKPSTLSFLPLREALSKPEPFMETDFAKIGRPGVLHQAFRGLDKYRADKGSLPEAGDMEQAEAVFELAKGCNEDGDFKAEGLDDSKDVILRLSLGARGVLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAFEALPEKPLAKEEVQPLGCRYDGSIMVFGKTMQALLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDGDGQVHVTDMDNIEKSNLSRQFLFRESDIGRAKSLTAARAVRAMNPSLNIKPYEAKCAQETEELFSDDFYSGLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLQDPNYTTFLNSQHNTKLETLTRISESLDSSRPSSFGDCIKWARLQFQARFHNEIAQLLHNFPVDSVTSSGNPFWSGAKRPPCPLDFDPNDALHMGFVKGAAVLRARMYGIKPPTDDAVYASTLSQMEVPVFKPVDGVKIATTEAEAKEQATGXXXXXAQLEDVDAQCERMLGELPKPADMKDFRLEVVEFDKDLDEHMEFVTAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVSMEVYKIMQEKPLESYKNWFLNLALPQFSCSEPLPPAK 988          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: A0A836CIM1_9STRA (Ubiquitin-activating enzyme E1 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CIM1_9STRA)

HSP 1 Score: 1203 bits (3112), Expect = 0.000e+0
Identity = 610/938 (65.03%), Postives = 718/938 (76.55%), Query Frame = 0

Query:   18 VDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQGMTELEGREFTITEKGPFSFEIDCDTSSCGTFVSGYVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRADKG-SLPEAGDMEHAEAVYGFAKACNEEGD--FKAEGLE--ESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKP----LSKEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQ-------LEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPPLRRPPSSRDRNGNGRRGIAWSLM 939
            VDESLYSRQLYVMG EAQRRMA S+VLIVG NGLG EVAKNVILAGVKSVTLLD   A WSDL+AQFYLTEAD+GKPRAAACV+KLAELNRYV  S    ++T  L+S FQ VV+ID  L  QL V+D CH KG+C I+ DVRGVFA  FCDFG AFVVSD +  QA SC+VS +TKD+  LVTVMDDQRH L TGDVV F SV+GM ELEGREFTITE+GP++FEI+CDT++ GTFVSGYVNQ    VKQP T++F+PLR AL++PEPFMETDFAK GRPG+LHQ FR L  +RA+ G +LP  G+   A+A+   A+  N      +KA+GL+  E+  V+  L+  +RG+++PVCA +GG+VGQEVLKA SGKF PIRQW+Y+DA EALP       L+ EEVQP  CRYDG IMVFGK +Q  LG Q LFLVGAGAIGCEMLKNWAMMGVGC  GG +HVTDMDHIE+SNLSRQFLFRE  IG+ KS TAA A +AMN  + +  +E KC+ +TE +F+DDFY++L+ VCTALDNVEARLYMDQ+CLFY+KPM ESGTLGTKGNTQ+VVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQW+RDWFEG F Q+A D N YL  P+F + L +Q N +L+TL R+ E+L SGRP  +  C+ WARL+F+  FHN +AQLLHNFP DS+TSSG PFWSGAKRPP P+ F+  D LH+ FV+ AA LRA  YGI    D++  +  LSS+ VP+F P +GVKIA TEAEAKE                 + D D QC  ++  LP+P  +  FRL  VEFDKD+D HM  V AASNLRAR YKIPEADMHRSR IAGKIIPAIATTTALVTGLVC+E+ K+ QEKPLE+YKNWFLNLALPQF+CSEP+PP     ++    G   R  AW  +
Sbjct:    3 VDESLYSRQLYVMGREAQRRMATSDVLIVGVNGLGVEVAKNVILAGVKSVTLLDRTPASWSDLAAQFYLTEADIGKPRAAACVNKLAELNRYV--SCVEEDLTTTLVSRFQVVVLIDVPLSVQLEVDDFCHSKGICCIAADVRGVFASVFCDFGPAFVVSDKNDTQAVSCLVSGVTKDSPALVTVMDDQRHQLETGDVVTFASVEGMPELEGREFTITERGPYAFEINCDTTAFGTFVSGYVNQ----VKQPVTVAFKPLRAALDQPEPFMETDFAKFGRPGVLHQGFRALHRYRAEHGGALPAPGNSAQADALLALARELNSGSSDGYKADGLDGAEAARVLRALASTSRGVVSPVCAAVGGVVGQEVLKAASGKFMPIRQWLYWDAEEALPAAAEGGVLAVEEVQPQNCRYDGQIMVFGKTLQAALGAQNLFLVGAGAIGCEMLKNWAMMGVGCGPGGAIHVTDMDHIERSNLSRQFLFREEHIGKPKSATAAAAARAMNGDMKLSAHELKCAPDTEGVFNDDFYAHLTGVCTALDNVEARLYMDQKCLFYKKPMFESGTLGTKGNTQVVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWARDWFEGAFSQSAGDANAYLEMPDFLAQLAAQPNARLETLRRVEEALVSGRPERYAQCVQWARLRFEDLFHNTVAQLLHNFPADSMTSSGTPFWSGAKRPPTPVAFDADDPLHVAFVRAAADLRAANYGIAAAADDAAVLAALSSVIVPDFTPAEGVKIAVTEAEAKEXXXXXXXXXXXXXXXXFMMDTDAQCAALVDALPEPASLGAFRLAAVEFDKDVDAHMVLVTAASNLRARCYKIPEADMHRSRLIAGKIIPAIATTTALVTGLVCLELIKVAQEKPLEAYKNWFLNLALPQFACSEPIPPAT---TAVKLKGREWRWSAWDCI 931          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: A0A7S2G964_9STRA (E1 ubiquitin-activating enzyme n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2G964_9STRA)

HSP 1 Score: 1043 bits (2698), Expect = 0.000e+0
Identity = 532/915 (58.14%), Postives = 656/915 (71.69%), Query Frame = 0

Query:   18 VDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQGMTELEGREFTITEKGPFSFEIDCDTSSCGTF-VSGYVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRADKG-SLPEAGDMEHAEAVYGFAKACNE---EGDFKAE----GLEESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLSKEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAG-------QLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPP 916
            +DE LYSRQLYVMG E Q RMA S+VLIVG NGLG E+AKNVILAGVKSVTL D     W DLSAQFYL EA+LG+PRAA    KLAELN+YV V+    E+    +S+FQ VVMI+A  + QL VND CH  G  +IS +V GVF   FCDFG  FVVSD +G  ASSC+V+SIT+ +  LVTV+DDQRH L TG VV  D + GM EL GR+FTIT K  +SFEIDCDT++   +  +GYVN+    +KQP+TL+F+ LRE+L  P     +DF K  RPGLLH AFR L+ +++  G + P  G++EHA  + G     NE   EG+FK E    G E +  V+ +LS+G+ G++NP+CA +GG+VGQEVLKACSGKF P+ Q+ Y+DA EALPD+PL+ ++V P GCRYD  I+VFG  MQE +     FLVG+GAIGCEMLKNWA+MG+     G VH+TDMD IEKSNLSRQFLFR SDIG+ K+  A    + MNP++ I  YEA+   ++E LF+DDF+  L  V  ALDN+EARLYMD RCLFY+KP+LESGTLGTKGNTQ+VVP+LTENYGA+RDPPEKSIPVCTLKNFPNQIEHTLQWSRD FEG FKQ  ED N YL  P+F   L  Q +TKLDTL  +  +L   R   F+DCI+W RL F+  ++N+I QLLHNFP D +TS+G PFWSG+KR P  L F+ +D LH+ ++K  A LRA  YGIK   D++ ++  L ++ VP F P DGVKI T E                     +L DVD QC+ ++ +LP P  +  F+L  VEFDKD+D+HMEFV AASNLRAR YKIPEADMHRSR IAGKIIPAIATTTALVTGLVC+E+YK++Q KP+E+YKN F+NLA+P F+ SEP PP
Sbjct:   20 IDEGLYSRQLYVMGREGQARMAVSDVLIVGMNGLGVEIAKNVILAGVKSVTLHDPTPTSWLDLSAQFYLGEAELGQPRAAVSAPKLAELNQYVKVTCRDDELNPEFLSNFQCVVMINAPYETQLAVNDYCHANGKQFISSEVAGVFGSVFCDFGPEFVVSDINGEAASSCIVASITQASPALVTVLDDQRHGLETGAVVQLDGLDGMEELNGRQFTITFKDAYSFEIDCDTTAMSPYKTAGYVNE----IKQPTTLAFKSLRESLTAPGEITLSDFGKWDRPGLLHMAFRALDVWKSKHGGAYPTPGNLEHAGELMGELTTLNEAAGEGEFKLEAEQLGSEATVKVVQQLSVGSAGVINPMCALLGGVVGQEVLKACSGKFMPLTQFFYFDATEALPDQPLTIDQVTPTGCRYDSQIVVFGSEMQEKIMALNYFLVGSGAIGCEMLKNWALMGLAAGESGHVHITDMDSIEKSNLSRQFLFRPSDIGKMKATAATAKAQEMNPAMKITVYEARVGSDSEGLFNDDFFEALDGVTNALDNIEARLYMDSRCLFYQKPLLESGTLGTKGNTQMVVPHLTENYGATRDPPEKSIPVCTLKNFPNQIEHTLQWSRDMFEGMFKQTMEDTNTYLTSPDFAETLRQQQSTKLDTLKNVKAALVDDRALSFDDCIAWTRLNFEVDYNNKIRQLLHNFPEDQVTSTGQPFWSGSKRAPVALVFDASDPLHLAYIKAGANLRASNYGIKGTWDDAYFLAFLPNVVVPGFTPKDGVKIKTPEEXXXXXXXXXXXXXXXXXXDEELMDVDAQCDEIIAQLPTPASLAGFQLNPVEFDKDIDEHMEFVTAASNLRARNYKIPEADMHRSRLIAGKIIPAIATTTALVTGLVCLELYKVIQSKPVEAYKNGFVNLAIPVFAFSEPQPP 930          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: A0A7S1TWZ4_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1TWZ4_9STRA)

HSP 1 Score: 1037 bits (2682), Expect = 0.000e+0
Identity = 535/936 (57.16%), Postives = 660/936 (70.51%), Query Frame = 0

Query:   18 VDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVAT-GEVTE-ALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQGMTELEGREFTITEKGPFSFEIDC-DTSSCGTFV-SGYVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEF-RADKGSLPEAGDMEHAEAVYGFAKACN---------EEGDFKAEGLEESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLSKEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPPLRRPPSSRDRNGNGRRGIAWSLM 939
            VDE LYSRQLYVMGHEAQRRMA S+VLIVG NGLG EVAKNVILAGVKS+TL D   A + DLSAQFYLTEADLG+PRAAA  +KLAELN YV VS+   G+  + A +S F+ VV ID  L EQLR+NDLC + G C+++ DVRGV A  FCDFG +F   D DG  A+S ++S++T++    VT ++D RH L TGD V    V+GM EL G+EF IT  GP++FE+   D +    ++  GYVNQ    VK+P  LSFR +REAL  P  F+  DFAK+ RP +LHQAFRGL  + R   G+LP  GD   AE V+  A+  N          E     E  E++KDV+LR++L A G+ +P+CA MGG++GQEVLKA SGKF PIRQW Y DA E L D+PL  EEV P G RYDG I V+G  +Q  +  Q++FLVGAGAIGCEMLKNWAMMGVGC   G VHVTDMD IE+SNLSRQFLFR +DIG+ KS TAA + K MN  + + PYEAK +  TE++F  DFY  L AVCTALDN++ARLYMD RCLF+  PM ESGT+GTKGNTQIVVP+LTE+YGA+RDPPEKS+PVCTLKNFP+QIEHT+QW+RDWFEG FKQ  + VN YL D  F   L  Q N +L+TLT +  SL   RP  F+DC++WARLQF + F N I QLLHNFP+D +T+SG PFWSG+K+PP PL F  +D +H+ F++  A LRA +YGI P TD+   +  L  ++VP F P DGVKIA  E EA E+       G    VD++  R++  LP P  +  FRL  V+FDKD+D+ M  V A SNLRAR YKIPEADMH SRQIAGKIIPAIATTTA+VTGL+ +E++K++Q+KPLE+YKN F NLA+P F+ SEP PP +     +   G  +   AW  +
Sbjct:   21 VDEGLYSRQLYVMGHEAQRRMASSDVLIVGLNGLGVEVAKNVILAGVKSLTLYDPTPASYGDLSAQFYLTEADLGQPRAAASKAKLAELNEYVQVSLTPEGQALDGAYLSGFRVVVAIDQLLAEQLRLNDLCRECGACFLAGDVRGVAAAIFCDFGASFTCYDVDGEPAASAMISAVTQENPAAVTCLEDHRHGLETGDFVTITGVEGMEELNGQEFQITVTGPYTFELPTVDATGYANYIRGGYVNQ----VKKPVELSFRTMREALVSPGEFLLADFAKMDRPPVLHQAFRGLHAYMREHGGALPPPGDAAAAEEVWQEAQRINAAAKTDGDGSEAALHVENFEDAKDVVLRVALVAAGVTSPMCAAMGGVLGQEVLKAASGKFCPIRQWFYIDALETLSDEPLPAEEVAPRGSRYDGQIAVYGATLQTKICAQRVFLVGAGAIGCEMLKNWAMMGVGCGDAGGVHVTDMDRIERSNLSRQFLFRSTDIGQPKSTTAAASAKRMNTDMRVSPYEAKVAPSTEDVFDTDFYDGLDAVCTALDNIDARLYMDSRCLFFGLPMYESGTMGTKGNTQIVVPHLTEHYGATRDPPEKSVPVCTLKNFPHQIEHTIQWARDWFEGAFKQTPDSVNAYLSDAEFLKKLERQPNIRLETLTAVMRSLVGDRPRSFDDCVAWARLQFDKLFRNAILQLLHNFPLDHVTTSGAPFWSGSKKPPTPLVFSTSDEVHLEFIRSVARLRAQIYGITPSTDDESIVAALDGIDVPAFSPADGVKIAADEKEAAEEEKSSLSGGTS--VDSEVSRIVDALPAPASLAGFRLSPVDFDKDVDEQMAVVMAVSNLRARNYKIPEADMHASRQIAGKIIPAIATTTAMVTGLISLEMFKVLQKKPLEAYKNGFANLAIPVFAFSEPGPPEKTKAMIK---GEEKAFTAWDFL 947          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: H3G877_PHYRM (E1 ubiquitin-activating enzyme n=33 Tax=Phytophthora TaxID=4783 RepID=H3G877_PHYRM)

HSP 1 Score: 1015 bits (2624), Expect = 0.000e+0
Identity = 534/926 (57.67%), Postives = 652/926 (70.41%), Query Frame = 0

Query:   10 DAAGSKVVVDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQG---MTELEGREFTITEKGPFSFEIDC-DTSSCGTF------VSGYVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRAD-KGSLPEAGDMEHAEAVYGFAKACNEEG----DFKAEGLE--ESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLS--KEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPP 916
            D +GS   +DE LYSRQLYVMG EAQ RM  SNVLIVG NGLG E+AKNVILAGVKSVTL DD  A   DL++QFYLTEAD+GKPRAA  ++KLAELN YV V   TGE+TE  +  F+AVV+++A L E  R+N +CH K + +I  + RGVF   FCDFG+ F+VSD DG +  SC++SSI+     LVTV DD RH L TGD+V+F  V G   + + + R+ T+T  GPF+F +D  D +    F        GYV Q    VKQP   SF+ L  AL  P  F+  DFAKIGR  LLH AF+ L+ F+   +GS P+ G M+ AE V   A   N++      F  E ++  +SK +I  L+ GA G++ P+ A +GGIVGQE LKACSGKF+PI+Q+ Y+DA E LPD   +   +E  P G RYDG I+VFG+ +QE +    LFLVGAGAIGCEMLKNWAMMGV  ++ G +H+TDMD IEKSNL+RQFLFR  D+ +AKS  AA A+K MNP +N++ Y ++   E+E+ F+DDF+ +LS VCTALDNVEARLYMDQRCLFY  PM ESGTLGTKGNTQ+VVP+ TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVN+YL  P F   LN Q NTK++TL R+  SL   RP  FEDCISWAR +F+  F N+I QLL NFPVD LT+SG PFWSG KRPP P+ F+  D LHM FV   A  RA  YG+K   D   + + L+S+ VPEF P  GVKIA ++AE KE    GG A  LEDVD QCE +L+ELPKP  +  +R+Q +EFDKD D HME + + SNLRAR YKIPE DMH+SR IAGKIIPAIATTTALVTGLVC E  K+ QEKPL+ YKN F+NLALP F+ +EP+ P
Sbjct:    4 DQSGS---IDEGLYSRQLYVMGREAQLRMGASNVLIVGLNGLGVEIAKNVILAGVKSVTLHDDTPASTLDLASQFYLTEADVGKPRAAVSLNKLAELNPYVPVRCHTGEITEDFLLGFRAVVLVNAPLTEAKRINAICHAKSIAFIMTEARGVFGSVFCDFGDEFIVSDRDGVEPVSCLISSISNTVPPLVTVNDDARHGLETGDIVSFREVSGFPFLNDSKPRKVTVT--GPFTFTLDTVDDADKERFDQGQPSSGGYVTQ----VKQPLVTSFKNLESALVAPGEFLINDFAKIGRSELLHVAFQALDAFQEKHQGSYPKPGSMQDAEEVSALASELNKQAISKKQFSVENVDAADSKKIIQALAAGALGVIAPMAAFLGGIVGQETLKACSGKFTPIQQFFYFDAVECLPDAVYAGTPDEFAPAGSRYDGQIVVFGRQLQEKIKSLNLFLVGAGAIGCEMLKNWAMMGVASNKNGIIHITDMDTIEKSNLNRQFLFRSKDVQQAKSSVAARAIKDMNPDVNVQAYVSRVGAESEDQFNDDFFESLSGVCTALDNVEARLYMDQRCLFYGLPMFESGTLGTKGNTQVVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNRYLEGPTFLKELNEQQNTKVETLERLKNSLVDDRPMSFEDCISWARFKFEDLFSNQIKQLLFNFPVDQLTTSGTPFWSGPKRPPTPITFDVKDPLHMDFVVSVANSRAKNYGLKGHADRDSFAQVLASIHVPEFSPRKGVKIAASDAELKE----GGAAPGLEDVDAQCEFILKELPKPSTLAGYRMQAIEFDKDDDAHMEVIVSVSNLRARSYKIPEEDMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKVFQEKPLDHYKNGFVNLALPLFTFAEPIEP 916          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: K3WKD4_GLOUD (E1 ubiquitin-activating enzyme n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WKD4_GLOUD)

HSP 1 Score: 1006 bits (2600), Expect = 0.000e+0
Identity = 527/926 (56.91%), Postives = 649/926 (70.09%), Query Frame = 0

Query:   10 DAAGSKVVVDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQG---MTELEGREFTITEKGPFSFEIDC-DTSSCGTFVSG------YVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRAD-KGSLPEAGDMEHAEAVYGFAKACNEE----GDFKAEGLE--ESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLS--KEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPP 916
            D AG K+  DESLYSRQLYVMGHEAQ RM  SNVLI+G NGLG E+AKN+ILAGVKSVTL DD  A   DL+AQFYLTE D+G+ RA A   KLAELN YV V   +GEVTE  +  F+AVV++++TL E LR+N +CH   + ++S + RGVF   FCDFG+ FVVSD DG Q  +C+V+SI+     LVTV DD RH L TGD V F  V+G   + E + R  T+T  GPF+F +D  D      F +G      YV Q    VKQP T+ F+PL EAL  P  F+ +DFAK  RP LLH AF+ L+ ++A  +G  P+ G ++ A+ VY  A   N+E    G F  + LE  E+K VI  L+ G+ G+++P+ A +GGIVGQE LKACSGKF+PI+Q+ Y+DA E LPD   S   +E  P G RYDG I+VFGK +QE +    LFLVGAGAIGCEMLKNWAMMGV   +   +H+TDMD IEKSNL+RQFLFR  D+ +AKS  A  ++K MNP +N+K Y A+   ++E  F+D+F+ +LS VCTALDNVEARLYMDQRCLFY  PM ESGTLGTKGNTQIVVP+ TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVNQYL  P F   LN Q NTKL+TL R+ +SL   RP+ FEDCISWAR +F+  F N+I QLL+NFP+D +T+SG PFWSG KRPP  + F+  D++H+ FV   A  RA  YG+K   D     K LS + VP F P DGVKIA ++AE KE       A ++++ D Q E +L  LPKP+ +  +R++ +EFDKD D HME + A SNLRAR YKIPEADMH+SR IAGKIIPAIATTTALVTGLVC E  K  Q+KPL+ +KN F+NLALP F+ SEP+ P
Sbjct:   32 DEAGPKI--DESLYSRQLYVMGHEAQMRMGASNVLIIGLNGLGVEIAKNIILAGVKSVTLHDDTPASHLDLAAQFYLTEEDIGQSRAQASSKKLAELNPYVHVQHHSGEVTEDFLKQFRAVVLVNSTLKESLRINQICHVHSIAFVSTEARGVFGSVFCDFGDEFVVSDRDGEQPVNCMVTSISNTTPPLVTVSDDTRHQLETGDYVTFREVKGFPFLNECKPRRVTVT--GPFTFTLDILDEEDEKLFANGSPSAGGYVTQ----VKQPLTVKFKPLTEALAAPGEFLISDFAKFNRPELLHFAFQALDAYQAKHQGGYPKPGSVQDADEVYAIAVNLNKEAAASGRFSIDHLETEEAKKVIRGLASGSSGVISPMAAFLGGIVGQEALKACSGKFTPIQQFFYFDAVECLPDSVYSGNTDEFAPTGSRYDGQIVVFGKTLQEKIRNLSLFLVGAGAIGCEMLKNWAMMGVSTGKDSVIHITDMDTIEKSNLNRQFLFRSKDVQQAKSTVAGRSIKEMNPEVNVKSYVARVGADSEAQFNDEFFESLSGVCTALDNVEARLYMDQRCLFYGLPMFESGTLGTKGNTQIVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNQYLSGPEFIKQLNDQQNTKLETLERLKQSLVDQRPASFEDCISWARFKFEDLFSNQIKQLLYNFPLDQVTTSGTPFWSGPKRPPTAITFDVNDSVHLDFVVAVANSRAKNYGLKGHHDREAIAKVLSGIHVPAFAPRDGVKIAASDAEMKED----SNASRVDNPDAQAEYILNALPKPDSLAGYRMEPIEFDKDDDSHMEVIVATSNLRARSYKIPEADMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKAFQDKPLDHFKNGFVNLALPLFTFSEPIEP 945          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: I2CSG2_NANGC (E1 ubiquitin-activating enzyme n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=I2CSG2_NANGC)

HSP 1 Score: 1006 bits (2600), Expect = 0.000e+0
Identity = 524/920 (56.96%), Postives = 654/920 (71.09%), Query Frame = 0

Query:   18 VDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEAL----ISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQGMTELEGREFTITEKGPFSFEIDCDTSSCGTFV-SGYVNQARINVKQPSTLSFRPLREALEKPE--PFMETDFAKIGRPGLLHQAFRGLEEFRAD-KGSLPEAGDMEHAEAVYGFAKACNE--EGDFKAEGLEESKD---VILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLSKEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDES---VYIKT-LSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQLE----DVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPP 916
            +DE LYSRQLYVMGHEAQ+RM  S+VLIVG  GLG EVAKNVILAGVKSVTL D     + DL+AQFYL+EADLGKPR  A   +LAELN YV V V   E  EAL    +  +Q + + +  L EQLR+N + H   + +I+ +VRG+    FCDFGEAF+VSD     A + ++ SIT++   +V+V+++ RH L TGDVV    V+GM E+  +E+ IT  GP +F+I   T     +V  GY+ Q    +KQ  TL+F+PL +AL       F+  DFAK+ RP LLH  FR L  F A  +G  P  G  E AE V   A + NE  EG+ +       KD   VI +L+LGA G+LNP+ +  GGIVGQEVLKACSGKFSPI+QW Y+DA E LP +P+S+ E  P+ CRYDG I VFGK++Q++LG+Q  FLVGAGAIGCEMLKNWAMMGVG    G VHVTDMD IEKSNLSRQFLFR +DIG+AKS+ A  A + MNP L I  YE +   +TE++F+D+FY  L+ VCTALDNV+ARLYMDQRCLFY+ PMLESGTLGTKGNTQ+VVP+LTENYGA+RDPPEKSIPVCTLKNFPNQIEHT+QW+RDWFEG FKQ  EDVN YL +P +   L +Q NTKL+TL RI  SL+  RP+ F+DC++WARL+F++ F+N I QLLHNFP+D +TS+G PFWSG K+PP PL F   D LH+ FV   A LRA MY +  P  E+   + +K  L ++ VP+F P DGVKIA T+ EAK+Q   GG  G       D++ +CE ++  LP P  +  F LQ ++FDKD+D HM  V A SNLRA  YKIPEADMH++RQIAGKIIPAIATTTALVTGLVC+E+YK++ +KP+E +K+ F+NLALP F+ SEP PP
Sbjct:   17 IDEGLYSRQLYVMGHEAQKRMQASDVLIVGVAGLGIEVAKNVILAGVKSVTLFDPAPVAYPDLAAQFYLSEADLGKPRDQASAPRLAELNPYVPVHVLEPEAGEALTAEAVKRYQVLCVTNRPLAEQLRLNAITHPLNIAFIASEVRGLCGSVFCDFGEAFLVSDPSDEPAINAMIGSITQEDPAVVSVLEEHRHGLETGDVVMIAEVEGMPEVNNQEYRITVTGPSTFQIPISTKGASPYVRGGYLQQ----IKQARTLTFQPLEKALGASGLGDFVLADFAKMDRPPLLHLGFRALHAFMAKHQGLAPVPGRREDAEEVLALAHSLNEATEGELQIPREVLEKDGARVIRQLALGAAGVLNPMASFFGGIVGQEVLKACSGKFSPIKQWFYFDALECLPTEPVSEAEAAPLNCRYDGQIAVFGKSVQQILGQQAYFLVGAGAIGCEMLKNWAMMGVGTGEKGAVHVTDMDRIEKSNLSRQFLFRANDIGQAKSVCAVRAAQTMNPDLKICAYENRVGADTEDIFNDEFYEQLTGVCTALDNVDARLYMDQRCLFYQLPMLESGTLGTKGNTQVVVPHLTENYGATRDPPEKSIPVCTLKNFPNQIEHTVQWARDWFEGVFKQAPEDVNAYLSNPEYLPHLQTQQNTKLETLRRIQASLND-RPTSFQDCLAWARLRFEELFNNTIRQLLHNFPIDQVTSTGTPFWSGPKKPPTPLSFHPDDPLHLAFVSSVAALRASMYALPAPPSEACAPLAVKAALENVMVPDFSPQDGVKIAVTDTEAKQQAEQGGSDGGAVTSSLDLEARCEAIIASLPPPSALTGFTLQPIDFDKDVDAHMAVVTATSNLRAANYKIPEADMHKTRQIAGKIIPAIATTTALVTGLVCLELYKLVLKKPIEDFKSAFVNLALPLFTFSEPQPP 931          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: A0A6A3N8N5_9STRA (E1 ubiquitin-activating enzyme n=4 Tax=Phytophthora TaxID=4783 RepID=A0A6A3N8N5_9STRA)

HSP 1 Score: 1003 bits (2594), Expect = 0.000e+0
Identity = 520/916 (56.77%), Postives = 643/916 (70.20%), Query Frame = 0

Query:   18 VDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQGMTEL-EGREFTITEKGPFSFEIDC-DTSSCGTFVSG------YVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRAD-KGSLPEAGDMEHAEAVYGFAKACNEEG----DFKAEGLE--ESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLSK--EEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPP 916
            +DE LYSRQLYVMG EAQ RM  SNVLI+G NGLG E+AKNVILAGVKSVTL DD  A   DL++QFYL+EAD+GK RAA  V KLAELN YV V   +GE+TE  +  F+AVV+++  L E  R+N +CH K + +I+ + RGVF   FCDFG+ F+VSD DG +  SC++SSI+  A  L+ V DD RH L TGD+V+F  V G   L +G+   +T  GPF+F +D  D +    F  G      YV Q    VKQP    F+ L  AL  P  F+ +DFAKIGR  LLH AF+ L+ ++   +G  P+ G M  A  V   A   N++      F  E ++  +SK VI  L+ GA G+L+P+ A +GGIVGQE LKACSGKF+PI+Q+ Y+DA E LP+   +   +E  PVG RYDG I+VFG+ +QE +    +FLVGAGAIGCEMLKNWAMMGV  ++   +H+TDMD IEKSNL+RQFLFR  D+ +AKS  AA A+K MNP +N++ Y ++   E+E+ F+DDF+ +LS VCTALDNVEARLYMDQRCLFYR PM ESGTLGTKGNTQIVVP+ TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVN+YL  P F   LN Q NTK++TL R+  SL   RP  FEDCISWAR +F+  F N+I QLLHNFP+D LT+SG PFWSG KRPP P+ F+  D LH+ F+   A  RA  YG+K  TD   + + L+ + VPEF P  GVKIA ++AE KE    GG A  LED D QCE +L ELPKP D+  +R++ +EFDKD D HME + + SNLRAR YKIPE DMH+SR IAGKIIPAIATTTALVTGLVC E  K+ Q+KPL+ YKN F+NLALP F+ +EP+ P
Sbjct:   77 IDEGLYSRQLYVMGREAQLRMGASNVLIIGLNGLGVEIAKNVILAGVKSVTLHDDAPATKLDLASQFYLSEADIGKSRAAVSVKKLAELNPYVPVRCHSGEITEEFLLGFRAVVVVNVPLKEAKRINAVCHAKSIAFIATEARGVFGSVFCDFGDEFIVSDRDGVEPVSCLISSISNSAPPLIAVSDDTRHGLETGDLVSFREVAGFPFLNDGKPREVTVTGPFTFTLDTIDDAGKRLFEEGQPSSGGYVTQ----VKQPLVAKFKDLESALATPGEFLISDFAKIGRSELLHVAFQALDAYQEKHQGRYPKPGCMRDAGEVLSLAAELNDQSASKKHFSVENIDADDSKKVIQALAAGATGVLSPMAAFLGGIVGQEALKACSGKFTPIQQFFYFDAIECLPETVYADTPDEFAPVGSRYDGQIVVFGRKVQEKIKNLNMFLVGAGAIGCEMLKNWAMMGVASNKDATIHITDMDTIEKSNLNRQFLFRSKDVQQAKSSVAARAIKDMNPDVNVQSYVSRVGAESEDQFNDDFFESLSGVCTALDNVEARLYMDQRCLFYRLPMFESGTLGTKGNTQIVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNRYLEGPAFMKELNEQQNTKVETLERLKSSLVDNRPLSFEDCISWARFKFEYLFSNQIKQLLHNFPLDQLTTSGTPFWSGPKRPPTPIAFDAKDPLHLDFIVSVANSRAKNYGLKGHTDRDTFTQVLAEIHVPEFSPRKGVKIAASDAELKE----GGAAPGLEDADAQCESILNELPKPSDLAGYRMEPIEFDKDDDSHMEVIVSVSNLRARSYKIPEEDMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKVFQDKPLDHYKNGFVNLALPLFTFAEPIEP 984          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: A0A3R7H2J5_9STRA (E1 ubiquitin-activating enzyme n=4 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7H2J5_9STRA)

HSP 1 Score: 998 bits (2579), Expect = 0.000e+0
Identity = 523/932 (56.12%), Postives = 648/932 (69.53%), Query Frame = 0

Query:    4 DPAEGEDAAGSKVVVDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQG---MTELEGREFTITEKGPFSFEIDC-DTSSCGTFVSG------YVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRAD-KGSLPEAGDMEHAEAVYGFAKACNEEGDFKAEGL------EESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLS--KEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPP 916
            D A G D  G+   +DE LYSRQLYVMG EAQ RM +SNVLIVG NGLG E+AKNV+LAGVKSVTL DD  A   DL++QFYLTEAD+GKPRA   V KLAELN YV V   +G +TE  +  F+AVV+++++L E  R+N +CH K + +I+ + RGVF   FCDFG+ FV+SD DG    SC++SSI+     LVTV DD RH L TGD+V+F  V G   + +   R+ T+T  GPF+F +D  D +    F  G      YV Q    VKQP    F+ L+ AL  P  F+  DFAKIGR  LLH AF+ L+ F+   +G  P+ G ME +E V   A   N++   K E +       ESK VI  LS G+ G+++P+ A +GGIVGQEVLKACSGKF+PI+Q+ Y+DA E LPD   +   EE    G RYDG I+VFG+AMQ+ + K  +FLVGAGAIGCEMLKNWAMMGV   +   +H+TDMD IEKSNL+RQFLFR  D+ +AKS  AA A+K MNP +N+  Y ++   E+E+ F+DDF+ +LS VCTALDNVEARLYMDQRCLFY  PM ESGTLGTKGNTQIVVP+ TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVN+YL  P+F   LN Q NTK++TL R+  SL   RP  FEDCISWAR +F+  F N+I QLL+NFP+D +T++G PFWSG KRPP P+ F+  D LHM FV   A  RA  YG+K   D + +   L+ + VP+F P  GV+IA ++AE KE    G     LED D QCE +L++LPKP  +  +R+Q +EFDKD D HME + + SNLRAR YKIPE DMH+SR IAGKIIPAIATTTALVTGLVC E  K+ Q+KPL+ YKN F+NLALP F+ +EP+ P
Sbjct:   47 DSAMGVDQTGT-ATIDEGLYSRQLYVMGREAQVRMGESNVLIVGQNGLGVEIAKNVVLAGVKSVTLHDDTPASSLDLASQFYLTEADIGKPRATVSVQKLAELNPYVPVRCHSGTITEDFLHGFRAVVLVNSSLKEAKRINAICHAKSIAFITTEARGVFGSIFCDFGDEFVISDRDGVDPVSCLISSISDSTPPLVTVSDDARHGLETGDLVSFREVPGFPFLNDSNPRKVTVT--GPFTFTLDIVDDADKFRFAQGKPSSGGYVTQ----VKQPLVTKFKDLKNALAAPGEFLINDFAKIGRSELLHVAFQALDAFQEKHQGIYPKPGCMEDSEEVIALALELNKQSAAKKEFIVDNITTAESKKVIQALSAGSTGVISPMAAFLGGIVGQEVLKACSGKFTPIQQFFYFDAVECLPDAVYAGTPEEFAAAGSRYDGQIVVFGQAMQDKIKKLNMFLVGAGAIGCEMLKNWAMMGVASGKDSAIHITDMDTIEKSNLNRQFLFRSKDVQQAKSSVAARAIKEMNPDINVHAYVSRVGAESEDQFNDDFFESLSGVCTALDNVEARLYMDQRCLFYGLPMFESGTLGTKGNTQIVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGDFFQAPSDVNRYLEGPDFMKELNEQQNTKVETLERLKNSLVDDRPMSFEDCISWARFKFEDLFSNQIKQLLYNFPLDQVTTTGTPFWSGPKRPPTPITFDLKDPLHMDFVVAVANSRAKNYGLKGHNDRTAFAHILNGIHVPDFSPQKGVRIAASDAELKEGAPPG-----LEDADAQCEFILKDLPKPSTLAGYRMQPIEFDKDDDSHMEVIVSVSNLRARSYKIPEEDMHKSRFIAGKIIPAIATTTALVTGLVCFEFLKVFQDKPLDHYKNGFVNLALPLFTFAEPIEP 966          

BLAST of mRNA_P-lacustris_contig938.15788.1 vs. uniprot
Match: A0A2D4BWE3_PYTIN (E1 ubiquitin-activating enzyme n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BWE3_PYTIN)

HSP 1 Score: 992 bits (2564), Expect = 0.000e+0
Identity = 514/924 (55.63%), Postives = 644/924 (69.70%), Query Frame = 0

Query:   10 DAAGSKVVVDESLYSRQLYVMGHEAQRRMAKSNVLIVGANGLGAEVAKNVILAGVKSVTLLDDGLAEWSDLSAQFYLTEADLGKPRAAACVSKLAELNRYVGVSVATGEVTEALISSFQAVVMIDATLDEQLRVNDLCHKKGVCYISCDVRGVFAYAFCDFGEAFVVSDTDGNQASSCVVSSITKDAIGLVTVMDDQRHNLVTGDVVAFDSVQGMTEL-EGREFTITEKGPFSFEIDC-DTSSCGTFVSG------YVNQARINVKQPSTLSFRPLREALEKPEPFMETDFAKIGRPGLLHQAFRGLEEFRADKGS-LPEAGDMEHAEAVYGFAKACNEEG----DFKAEGLE--ESKDVILRLSLGARGMLNPVCATMGGIVGQEVLKACSGKFSPIRQWMYYDAAEALPDKPLS--KEEVQPVGCRYDGSIMVFGKAMQELLGKQKLFLVGAGAIGCEMLKNWAMMGVGCDRGGQVHVTDMDHIEKSNLSRQFLFRESDIGRAKSLTAAGAVKAMNPSLNIKPYEAKCSQETEELFSDDFYSNLSAVCTALDNVEARLYMDQRCLFYRKPMLESGTLGTKGNTQIVVPYLTENYGASRDPPEKSIPVCTLKNFPNQIEHTLQWSRDWFEGCFKQNAEDVNQYLGDPNFPSFLNSQHNTKLDTLTRISESLDSGRPSGFEDCISWARLQFQQRFHNEIAQLLHNFPVDSLTSSGNPFWSGAKRPPCPLDFEQTDALHMGFVKGAAVLRALMYGIKPPTDESVYIKTLSSMEVPEFKPVDGVKIATTEAEAKEQVTGGGGAGQLEDVDTQCERMLRELPKPEDMKDFRLQVVEFDKDLDDHMEFVAAASNLRARVYKIPEADMHRSRQIAGKIIPAIATTTALVTGLVCMEIYKIMQEKPLESYKNWFLNLALPQFSCSEPLPP 916
            D  G K+  DE LYSRQLYVMG EAQ RM  ++VLI+G NGLG E+AKN+ILAGVKSVTL DD      DL+AQFYLTE D+G  RA   V KLAELN YV V    G++TE LI  ++ VV++++T  E LR++ +CH   + +I  D RGVF   FCDFG  FVVSD DG Q  +C+VSSI+     +VTV DD RH L TGD V+F  + G   L +G    +T  GPF+F +D  D +    FV+G      Y+ Q    VKQP T+SF+PL +AL  P  F+ +DFAK+  P LLH A R L+ F+   G   P+ G +   E VY  A   N +G    +F AE LE  E+K +++ L+ G+ G++ P+ A +GG+VGQE LKACSGKF+PI+Q+ Y+DA E LPD       +E    G RYD  I VFG+ +Q+ +  Q +FLVGAGAIGCEMLKNWAMMGV C + G +H+TDMD IEKSNL+RQFLFR  D+ + KS  AA A+K+MNP +N++ Y ++  QETE  F+DDF+ +LSAVCTALDNVEARLYMDQRCLFY  PMLESGTLGTKGNTQ+VVP+ TENYGASRDPPEKSIP+CTLKNFPN IEHTLQW+RDWFEG F Q   DVNQYL   +F   LN Q NTKL+TL R+ +SL   +P  FE+CI+WARL+F+  F N+I QLL+NFP+D +T+SG PFWSG KRPP P+ F+ +D +H+ FV   A  RA +YG+K   D + +++ + S+ VP F P DGVKIA+TEAEAKE            + D QC R++ +LP P  +  +R+  VEFDKD D HME + A SNLRAR YKIPEADMH+SR IAGKIIPAIATTTALVTGLVC E  K++Q +PLESYKN F+NLALP F+ SEP+ P
Sbjct: 1685 DECGPKI--DEGLYSRQLYVMGREAQMRMGTASVLIIGLNGLGVEIAKNIILAGVKSVTLHDDEPTSHLDLAAQFYLTEQDVGHARAERSVKKLAELNPYVEVDTHRGKITEELILKYRVVVLVNSTYSEALRIDKICHANSIAFIKTDARGVFGSVFCDFGPEFVVSDRDGEQPVTCMVSSISNTVPPVVTVHDDARHQLETGDYVSFREITGFPFLNDGTPRRVTVTGPFTFTLDVEDEADKQRFVAGETSAGGYITQ----VKQPLTVSFKPLSDALVAPGEFLISDFAKLNHPELLHIASRALDAFQEKNGGRYPKPGCLADGEEVYKIALDLNRDGASKKEFHAEQLESEEAKKIVIGLASGSTGVIAPMAAFLGGVVGQEALKACSGKFTPIQQFFYFDAVECLPDAVFDGVSDEFSLTGTRYDAQIAVFGRTLQDKIKNQSMFLVGAGAIGCEMLKNWAMMGVACAKNGVIHITDMDTIEKSNLNRQFLFRSKDVQQPKSSVAARAIKSMNPDVNVQSYVSRVGQETEGQFNDDFFESLSAVCTALDNVEARLYMDQRCLFYGLPMLESGTLGTKGNTQVVVPHKTENYGASRDPPEKSIPICTLKNFPNAIEHTLQWARDWFEGEFFQAPSDVNQYLAGGDFMKQLNEQQNTKLETLDRLKQSLVDQKPLSFEECIAWARLKFEDLFSNQIKQLLYNFPLDQVTTSGTPFWSGPKRPPTPITFDPSDPVHLDFVIAVANTRAAVYGLKGHHDRAAFVEAVKSISVPAFVPRDGVKIASTEAEAKEDAP-------ALNTDEQCXRIVEQLPSPASLAGYRMAPVEFDKDNDAHMEVIVATSNLRARNYKIPEADMHKSRFIAGKIIPAIATTTALVTGLVCFEFIKVLQSRPLESYKNGFINLALPLFAFSEPIAP 2595