prot_P-lacustris_contig92.15647.1 (polypeptide) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: D7FUP7_ECTSI (DNA topoisomerase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUP7_ECTSI)

HSP 1 Score: 993 bits (2566), Expect = 0.000e+0
Identity = 483/522 (92.53%), Postives = 497/522 (95.21%), Query Frame = 0

Query:  200 NRFDELQEGVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVGELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQEDEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVKNEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSGRPLLFXXXXXTGFPLPHKGKLAKLDPEFECPLCNFQ-------VLSVSPGNDYTGSGYTVCPHCFNN 714
            NRFDELQEGVISYGPCQFPTLGF+VDRYKRIQAFQ EAFWSIKMEYAEDDP SPGGKARADF+W+RGRLYDHACCA+LYEMTVEDGVAVVTSVEARR+TKARP+PMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDIN LLGDFRDNPRFGNYVG LLD+GGFLWPREGRGDD AHPPIHPT SVDPNSLQ DEKKIYELVTKHFLACCSKDAVGHQTKV ANCGGEDFTATGLMVTERNWL +YEPFERWTGRNIPEFFQGQEF PSVLVMDEG TQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKY VKNEQDRF+PTTLGLALVDGYD+IGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCIS MK+CF+TC A AHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSGRPLLFXXXXXTGFPLPHKGKL K DPEFECPLC+FQ       VLSVS GNDYTGSGYTVCP+CFNN
Sbjct:    3 NRFDELQEGVISYGPCQFPTLGFIVDRYKRIQAFQSEAFWSIKMEYAEDDPRSPGGKARADFSWQRGRLYDHACCAILYEMTVEDGVAVVTSVEARRVTKARPIPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINTLLGDFRDNPRFGNYVGGLLDNGGFLWPREGRGDDSAHPPIHPTKSVDPNSLQADEKKIYELVTKHFLACCSKDAVGHQTKVLANCGGEDFTATGLMVTERNWLDVYEPFERWTGRNIPEFFQGQEFVPSVLVMDEGRTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYAVKNEQDRFYPTTLGLALVDGYDNIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISKMKECFQTCQALAHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSGRPLLFXXXXXTGFPLPHKGKLTKQDPEFECPLCHFQARFLHVLVLSVSTGNDYTGSGYTVCPYCFNN 524          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: A0A4D9CZ97_9STRA (DNA topoisomerase n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CZ97_9STRA)

HSP 1 Score: 701 bits (1810), Expect = 6.420e-237
Identity = 379/770 (49.22%), Postives = 496/770 (64.42%), Query Frame = 0

Query:    1 MAVITAINVAEKPSVAKELANILGGGNS--RRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALFTAPLIRYVKEDKKDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNRNIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQEGVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYA---EDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVGELL----DDGG----------FLWPREGRGDDGAHPPIHPTTSVDPNSLQ-EDEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPE--FFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVKNE------------QDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHFRPLGSSGGESRTVRGGR------GFSRCGVCGTAMDLKKSSSGRPLLFXXXXXTGFP--LPHKGK----LAKLDPEFECPLCNFQVLSVSP---------GNDYTGSGYTVCPHCFNNP 715
            MA +  +NVAEKPSVAKE++ IL GGN   RRRQG S YNPI+EF  C + G+ V+M  TSV GHL+E+EF EPY K W AC+P  LFT P+ + V +DK+ LE+ L  EAR CQ L+LWLDCD EGENIA+EV++VC   N  +R+ RARFSALIPR+I +++++LV PN +Q++AV AR EIDLR+G+AFTRFQT++LQ +FD +++ +ISYGPCQFPT+GFVVDRYK I++FQ E FW I+M Y    E+    P G  R  F WKR RL+D A CAVLY M VE   A VT V A   T+ RP+P+STVELQK+ASRF ++SS+  MHVAE LY +G ISYPRTETE F R+ D+ +L+ +   +P +G +  EL+    ++GG          F +PR+G  DD AHPPIHPT    P  L+  DE+K+YELV +HFLACCS DA G +TKV    GGE+FTATG+++ ERNWL +Y  +E+W    +P      G  F P+ L ++ G TQ PPLLSE+DLI  MD+KGIGTDATIA+HI+ I QRKY                 Q RF PT LGL LV+GY+ IG+Q++ P LRA ME +C R++RGE  +  +V+ C+  MK+CFE C     +L +AV  + R  G  GG       G       GFS+CG C   MDL++  S   +LF           LPH  K     + L P   CPLC F+ L ++P         G++     Y +CP C+++P
Sbjct:    1 MAPVKVLNVAEKPSVAKEISAILSGGNRAPRRRQGGSDYNPIWEF-GCQIRGELVDMVFTSVRGHLLEMEFVEPYCKNWHACRPGDLFTIPVEKKVGKDKEGLERCLKSEARACQRLILWLDCDLEGENIAFEVMDVCRAANSRLRVERARFSALIPREIWHALEHLVEPNVYQSEAVNARMEIDLRIGAAFTRFQTLKLQGKFDGVEDSIISYGPCQFPTMGFVVDRYKAIESFQREEFWRIQMAYRGARENGDEGPQGFTR--FQWKRERLFDEAACAVLYVMVVEAKEARVTYVNAAPATRDRPLPLSTVELQKKASRFFNMSSDVCMHVAEELYTKGFISYPRTETESFSRDADLRSLIAEQGRHPAWGAFARELVGGGEEEGGQHGRDSGAGKFRFPRKGSKDDQAHPPIHPTKLAAPQELKGTDEQKVYELVVRHFLACCSDDAKGKRTKVTVEMGGEEFTATGIVILERNWLDVYNGWEKWHASTLPANVLQVGAAFVPTSLTLETGRTQPPPLLSEADLISRMDEKGIGTDATIAQHIKTILQRKYVTXXXXXXXXXXXXXXLQRRFQPTNLGLGLVEGYEEIGYQLTKPELRAEMERECKRVARGERGRTEMVQACLLKMKECFEDCQNQWERLPEAVGKYLRRYGQGGGREXXXXXGTIRTMQAGFSQCGHCQGRMDLRRDKS---VLFLACETCAASHLLPHSRKGTSRPSTLTPPVLCPLCGFEALLITPEGGGREGGQGDERVPPPYHMCPWCYHHP 764          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: A0A6A5C7F2_NAEFO (DNA topoisomerase n=1 Tax=Naegleria fowleri TaxID=5763 RepID=A0A6A5C7F2_NAEFO)

HSP 1 Score: 654 bits (1686), Expect = 6.270e-221
Identity = 352/718 (49.03%), Postives = 471/718 (65.60%), Query Frame = 0

Query:    7 INVAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALF--TAPLIRYVKEDKKDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNR-----NIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQEGVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKR--EFDINALLGDFRDNPRFGNYVGELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQEDEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVKNEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSGRPLLFXXXXXTGFPLPHKGKLAKLDPEFECPLCNFQVLSVSPGNDYTGSGYTVCPHCFNNP 715
            + VAEKP  AK++A IL G N +RR+G+S++N +FEFE   M GQ V M ITSV GHLMEL+F   + + W +C PI L   + P+IR V E   ++EK L  EAR    L+LWLDCDREGENIA+EVIEVC R NR     N  I RARFS   P  I  +++NL  PN   + AV+ARQE+DLR+G++FTRFQ+MRLQ +F  L   VISYGPCQFPTLGFVV R   I+ F+ E FW++   +        G + +A F WKRGRL+DH  C VLYE+ VE+  A VT V+ +R TK RPVP++T+ELQK AS+   +SS  TM VAE+LY +G ISYPRTET  + +  + D+  L+ +   NP +G Y  +LL+ GG+  P  G+ DD AHPPIHP       + Q+ E+ +YEL+++HFLACCS+DA+G +TKVH     E+FT +GLMV ++N+L I++ +++W+ ++IP F Q Q+F P+ L+MDEG T+ PP LSESDLI  MD  GIGTDAT+A+HI+ IQ R Y +K   +RF  T LG+AL+ GYD IG+++S P LRA ME D   IS+G+ TK+AV+ + ++  K  F     +AH LD AV  HF+P+G SG     VR    FS CG CG +MDLK+   G   LF       + LP K +   +  +  CP+CNFQVL V+   +   + Y +CP+CFNNP
Sbjct:    8 LGVAEKPEAAKQIATILSGNNFKRREGKSKFNKVFEFE-YRMNGQVVTMCITSVLGHLMELDFEGKF-RSWASCDPIVLMDVSTPVIRTVPEKNAEIEKNLENEARNASKLILWLDCDREGENIAFEVIEVCKRVNRTLSIENNTILRARFSEFTPAAINRAIRNLERPNVLDSMAVDARQELDLRIGASFTRFQSMRLQKKFHGLPGDVISYGPCQFPTLGFVVKRDLLIKNFESEKFWTLNCSH------DMGEEGKAQFLWKRGRLFDHLTCLVLYELCVENPTATVTRVDKKRRTKQRPVPLNTIELQKLASQKLRMSSHDTMKVAESLYNKGFISYPRTETNNYNQFSDDDLKNLIQEHSTNPVWGQYASDLLN-GGYRKPTSGKQDDKAHPPIHPLKGSCQTNDQK-EQDLYELISRHFLACCSQDALGSETKVHIVIANEEFTCSGLMVEQKNYLEIWK-YDKWSDKSIPVFQQNQQFVPTSLLMDEGETRPPPHLSESDLISAMDSNGIGTDATVAQHIKTIQDRGYVIKRG-NRFEATNLGIALLQGYDLIGYELSTPNLRAKMEKDMMCISQGQITKDAVINQSLAMYKHVFMQICKSAHLLDKAVQQHFKPIGESGQSKVLVRS---FSMCG-CGNSMDLKEQD-GHRFLFCKNCKEAYNLPSKCEYESISDQ--CPICNFQVLDVTTQKN---TNYKICPNCFNNP 703          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: A0A024TEB4_9STRA (DNA topoisomerase n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TEB4_9STRA)

HSP 1 Score: 656 bits (1692), Expect = 1.080e-220
Identity = 362/734 (49.32%), Postives = 463/734 (63.08%), Query Frame = 0

Query:    7 INVAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALFTAPLIRYVKEDKKDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNRNIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQ----------EGVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVGELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQEDEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVK-NEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHFRPLGSSGGESRTVRGGR-----------GFSRCGVCGTA-MDLKKSSSGRPLLFXXXXX--TGFPLPHKGKLAKLDPEFECPLCNFQVLSVSPGNDYTGSGYTVCPHCFNNP 715
            +NVAEKPSVAKE++ IL  G  RRR G+S YN IFEF    +  Q+V M  TSVTGHLMEL+FA  Y + W +C P+ LFTA + + V+ D+K +E  L  EA   QWL+LWLDCDREGENIA+EV  VC   NR +R+FRARFSALIPRDI ++VQ+LV PNE+ + A +AR EIDLRLG+AFTRFQTMRLQ +F ++             V+S+G CQFPTLGFVVDRY  I++F  E F+SI + +A  D  S        F+W R RLYDH     L+E  VE  VA VTS+  +  +K +P P++TVE QKRA+R+  I+SE  M  AE+LY +G++SYPRTET+ FK   D++ALL     +  +G YV +LL  G F  PR G+ DD AHPPIHPT +V   SL + E+K+YE VT HFLACCS DA GHQT VH     E F+A+GLM+  RN+L IY+ FE W+   IP + QG  FAP+ L +  G T  PPLL+ESDLI  MD  GIGTDATIAEHI  I +R+Y +K N   +F PT LGLALV  Y+ +G+Q++ P LRAAME DCT IS+G  + + V+  C+  M+  FE+ +  +  LDD    +F      GG     R               FSRCG CG + M+ +KS+     LF       T F LP    +  +   F CPLC FQV+ +    +     YTVCPHCFN+P
Sbjct:    4 LNVAEKPSVAKEISAILSQGGCRRRPGQSPYNAIFEFP-YQINNQNVEMAFTSVTGHLMELDFAASY-RSWKSCDPVELFTAKVEKKVRGDQKAIEGTLRTEAARAQWLILWLDCDREGENIAFEVKSVCEIVNRRLRVFRARFSALIPRDIQHAVQHLVQPNENLSLACDARSEIDLRLGAAFTRFQTMRLQKKFPQIHAQNNPANAGDNKVVSFGSCQFPTLGFVVDRYLAIESFVSEPFYSIHVTHAIPDVGS------ITFSWARNRLYDHLATLALFEACVEAKVATVTSITRKDTSKRKPFPLTTVEFQKRAARWLRITSEEAMAAAESLYNKGLLSYPRTETDSFKEGTDLSALLLLHLQHDSWGGYVSQLLHHGKFEHPRSGKHDDQAHPPIHPTKAVPLASLHDKERKVYEFVTLHFLACCSMDAKGHQTTVHMQMADEHFSASGLMILARNYLDIYK-FETWSNSVIPVYDQGDVFAPTSLTLQAGATTPPPLLTESDLIAKMDTHGIGTDATIAEHINTILKREYAIKVNNNTQFKPTALGLALVQSYNKMGYQLAKPDLRAAMERDCTAISQGHKSMDEVIRSCMIQMRDIFESVVHKSSVLDDTFGQYFGGA-VHGGNHHAARDAAMDVANSQLMASQFSRCGTCGCSEMEWRKSNHDTTNLFLSCPRCVTTFALPMHSSVVAMAGTF-CPLCRFQVVEIHRVTN-PQHPYTVCPHCFNHP 725          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: W4FMS7_9STRA (DNA topoisomerase n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FMS7_9STRA)

HSP 1 Score: 655 bits (1691), Expect = 2.110e-220
Identity = 363/737 (49.25%), Postives = 466/737 (63.23%), Query Frame = 0

Query:    7 INVAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALFTAPLIRYVKEDKKDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNRNIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQ--------------EGVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVGELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQEDEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVK-NEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHF------RPLGSSG-GESRTVRGGRGFSRCGVCGTA-MDLKKSSSGRPLLFXXXXXT--GFPLPHKGKLAKLDPEFECPLCNFQVLSVSPGNDYTGSGYTVCPHCFNNPRRP 718
            +NVAEKPSVAKE++ IL  G  RRR G+SQYN IFEF    +  Q+V+M  TSVTGHLMELEFA P  + W +C P+ LFTA + + V+ D+K +E  L  EA   QWLVLWLDCDREGENIA+EV  VC   NR +R+FRARFSALIPRDI ++VQ+LV PNE+ + A +AR EIDLRLG+AFTRFQTMR+Q +F  +                 V+S+G CQFPTLGFVVDR+  I+AF  E F+SI + +A  D  S        F W R RLYDH     L+E  VE  VA VTS+  +  +K +P P++TVE QKRASR+  I+SE  M  AE+LY +G++SYPRTET+ FK   D+ ALL     +  +G YVG+LL    +  PR G+ DD AHPPIHPT SV   SL + E+K+YE VT HFLACCS DA GHQT VH     E F+A+GLM+  RN+L IY  FE W+   IP + +G  F P+ L +  G T  PPLL+ESDLI  MD  GIGTDATIAEHI  I +R+Y +K N   +F PT LGLALV  YD +G+Q++ P LRAAME DCT IS+G+ T + V+  C++ M+  FE+ ++ ++ LD+    +F       P  +S   +S  V   +  SRCG CGT+ M+ +KS      LF         + LP    +  +   F CPLC FQV+ +    +     Y VCPHCFN+P  P
Sbjct:    4 LNVAEKPSVAKEISAILSQGGCRRRAGQSQYNAIFEFP-YQINNQNVDMAFTSVTGHLMELEFA-PSHRSWKSCDPVELFTAKIEKKVRGDQKPIEATLRTEAARAQWLVLWLDCDREGENIAFEVKSVCENVNRRLRVFRARFSALIPRDIQHAVQHLVQPNENLSLACDARSEIDLRLGAAFTRFQTMRIQKKFPRIHTQNAASSSSSSNTDNKVVSFGSCQFPTLGFVVDRFLAIEAFVSEPFYSIHVAHAIPDIGSIA------FTWARHRLYDHVATLALFETCVEAKVATVTSITRKDTSKRKPFPLTTVEFQKRASRWLRITSEEAMAAAESLYNKGLLSYPRTETDSFKEGTDLTALLTLHLTHDTWGGYVGQLLHHAKYEHPRTGKHDDHAHPPIHPTKSVALTSLHDKERKVYEFVTLHFLACCSMDAKGHQTTVHMQMADERFSASGLMILARNYLDIYR-FESWSNSVIPVYDKGDVFVPTALTLQAGETAPPPLLTESDLIAKMDMHGIGTDATIAEHINTILKREYSIKVNNNTQFKPTDLGLALVQSYDKMGYQLAKPDLRAAMERDCTAISQGQKTMDQVIRSCMTQMRTIFESVVSKSNILDETFGHYFGIHRTAHPHNNSDIAQSSQVMASQ-VSRCGTCGTSNMEWRKSLDDPSTLFLSCPRCMKSYGLPMHSSVVAMANTF-CPLCQFQVVEIHRVTN-PQHPYPVCPHCFNHPPNP 728          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: H3G7Y5_PHYRM (DNA topoisomerase n=2 Tax=Phytophthora TaxID=4783 RepID=H3G7Y5_PHYRM)

HSP 1 Score: 633 bits (1632), Expect = 1.590e-217
Identity = 335/618 (54.21%), Postives = 431/618 (69.74%), Query Frame = 0

Query:    8 NVAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALFTAPLIRYVKEDK--KDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNRNIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQE--GVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDD-PASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVG-ELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQE-DEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVK-NEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHF 617
            NVAEKPSVAKE+ANIL  G S+RR G S+YN +FEF    + GQ V M +TSVTGH+MEL+F +   + W +C P+ LFTAP+ + ++ D+  K +EK L+EEAR  QWLVLWLDCDREGENIAYEV  +C + NR +R+FRARFSALIPRDI+++VQNL  PNE  + AV+AR EIDLR+G+AFTRFQTMR+Q++F  +++   VISYGPCQFPTLGFVV+R+ +IQ F+ EAF  I + + ++D P +P       F WKRG L+D   C  +YE  VE   A + SV+ R   K +P+P++TVELQKRASR+  ISSE TM  AE+LY +G ISYPRTET KFK   D+ +L+     + ++GNYV  +LLD G +  PR G  DD AHPPIHPT S+D N+L++ +EKK+YE V  HFLACCS+DA G QT V     GE FTA+GLMV ERN+L IY+ +E+W    IP + +G  F PS +VM  G T  PPLLSE+DLI  MD  GIGTDATIAEHI+ I +R+Y +K N   +F PTTLGLALV  Y+ +G +++ P LRAAME DC  IS G+   + VV+ C+  MK  F     +A  LD   +  F
Sbjct:    4 NVAEKPSVAKEIANILSSGRSQRRAGFSKYNALFEFP-YEIRGQQVQMVVTSVTGHIMELDF-DASVRSWHSCDPVELFTAPVTKRIRGDETQKKIEKTLLEEARQSQWLVLWLDCDREGENIAYEVKAICEKSNRRLRVFRARFSALIPRDIVHAVQNLAEPNEKLSLAVDARSEIDLRIGAAFTRFQTMRIQSKFPAIKQENSVISYGPCQFPTLGFVVERFLKIQNFEREAFHDISVTHQQNDQPDAP----TTTFKWKRGHLFDRMACLCIYECLVESQTATIESVDKRPSWKRKPLPLTTVELQKRASRWLRISSEMTMKAAESLYNKGFISYPRTETSKFKEGTDLMSLVRLHETHQQWGNYVTRQLLDGGKYEAPRHGNSDDQAHPPIHPTKSIDVNTLRDPNEKKVYEFVALHFLACCSRDAKGSQTSVVMKILGESFTASGLMVEERNYLDIYK-YEKWNASAIPVYQRGDVFRPSTVVMLSGETNPPPLLSEADLISKMDSNGIGTDATIAEHIKTILKREYSIKVNNDTQFKPTTLGLALVLSYEHMGLELAKPVLRAAMENDCKAISLGQKDVQQVVQSCMDQMKSIFLEVTRSASVLDQTFSEQF 614          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: UPI001CFD67DF (uncharacterized protein n=1 Tax=Naegleria lovaniensis TaxID=51637 RepID=UPI001CFD67DF)

HSP 1 Score: 645 bits (1664), Expect = 4.650e-217
Identity = 345/718 (48.05%), Postives = 469/718 (65.32%), Query Frame = 0

Query:    7 INVAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALF--TAPLIRYVKEDKKDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNR-----NIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQEGVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKR--EFDINALLGDFRDNPRFGNYVGELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQEDEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVKNEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSGRPLLFXXXXXTGFPLPHKGKLAKLDPEFECPLCNFQVLSVSPGNDYTGSGYTVCPHCFNNP 715
            + VAEKP  AK++A IL G   ++R+G+S++N +FEF+   M GQ V M ITSV GHLMEL+F   + + W +C P+ L   + P+IR V E   D+EK L  EAR    L+LWLDCDREGENIA+EV++VCTR NR     N  I RARFS   P  I  +++ L  PN   + AV+ARQE+DLR+G++FTRFQ+MRLQ +F  L   VISYGPCQFPTLGFVV R   I+ F+ E FW++   +        G + +A F WKRGRL+DH  C +LYE+ VE+  A VT V+ +R TK RP+P++T+ELQK AS+   +SS  TM VAE+LY +G ISYPRTET  + +  + D+  L+ +   NP +G Y  +LL+ GG+  P  G+ DD AHPPIHP       +  + E+ +YEL+++HFLACCS+DA+G +TKVH     E+FT +GLMV ++N+L I++ +++W+ ++IP F Q Q+F P+ L+MDEG T+ PP LSESDLI  MD  GIGTDAT+A+HI+ IQ R Y +K   +RF  + LG+AL+ GYD IG+++S P LRA ME D   IS+G+ TK+AV+ + ++  K  F     +AH LD AV  HF+P+G SG     VR    FS CG CG +MDLK+   G   LF       + LP K +   +  +  CPLCNFQVL+V+       S Y +CP+CFNNP
Sbjct:    8 LGVAEKPEAAKQIATILSGNKFKKREGKSKFNKVFEFD-YRMNGQVVTMCITSVLGHLMELDFEGKF-RSWASCDPMVLMDVSTPVIRTVPEKHTDIEKNLENEARNASKLILWLDCDREGENIAFEVVDVCTRVNRALSIDNNTILRARFSEFTPAAINRAIRTLERPNILDSMAVDARQELDLRIGASFTRFQSMRLQKKFHGLPGDVISYGPCQFPTLGFVVKRDLLIKNFESEKFWTLNCCH------DMGEEGKAQFLWKRGRLFDHLSCLILYELCVENPTATVTRVDKKRRTKQRPIPLNTIELQKLASQKLRMSSHDTMKVAESLYNKGFISYPRTETNNYNQFSDDDLKNLIQEHSSNPIWGQYAADLLN-GGYRKPTSGKQDDKAHPPIHPLKGSC-QAPDQKEQDLYELISRHFLACCSQDALGSETKVHIVVANEEFTCSGLMVEQKNYLEIWK-YDKWSDKSIPVFQQNQQFVPTSLLMDEGETRPPPHLSESDLISAMDSNGIGTDATVAQHIKTIQDRGYVIKRG-NRFEASNLGIALLQGYDLIGYELSTPNLRAKMEKDMVCISQGQITKDAVINQSLAMYKHVFMQICKSAHLLDKAVQQHFKPIGESGQSKVLVRS---FSMCG-CGNSMDLKEQD-GHRFLFCKNCKEAYNLPSKCEYESISDQ--CPLCNFQVLNVTTQK----SNYNICPNCFNNP 702          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: A0A1V9Y6X7_9STRA (DNA topoisomerase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9Y6X7_9STRA)

HSP 1 Score: 645 bits (1663), Expect = 1.060e-216
Identity = 353/723 (48.82%), Postives = 460/723 (63.62%), Query Frame = 0

Query:    7 INVAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALFTAPLIRYVKED--KKDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNRNIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQEG----VISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVGELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQED-EKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVK-NEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHFRPLGSSGGESRTVRG---GRGFSRCGVCGTAMDLKKSSSGRPLLFXXXXXTGFPLPHKGKLAKLDPEFECPLCNFQVLSVSPGNDYTGSGYTVCPHCFNNPRRP 718
            +NVAEKPSVAKE+A IL  G+S+RR G SQYN IFEF    M  Q V M +TSVTGHLM L+FA  Y + W +C P+ LF+A + + V+ED  +K +EK L  EA   Q LVLWLDCDREGENIA+EV  VC + NR +R+ RARFSALIPRDI +++ NLV PNE+ A A +AR EIDLRLG+AFTRFQTMR+Q +F  +Q      V+S+G CQFPTLGFVVDRY  I++F  E F+ + + +   D  S        F W R R+YDH  C  LYE  ++  +A +TS+  R  +K +P P++TVE QKRA+R+  ISSE+ M  AEALY RG++SYPRTET+ FK   D+ ALL    ++  +G YV  LL +G +  PR G+ DD AHPPIHPT SV  +S+  D E+K+YE V  HFLACCS DA G QT V      E F+A+GLM+ ERN+L IY  +E+W    IP + +G +F P+++ +  G T  PPLL+ESDLI  MDQ GIGTDATIAEHI  I +R+Y +K N   +F PT LGLALV  YD +G+Q++ P LRAAME DC  I++G+ T   VV+ C+  M++ FE+ +  +  LD+    +F   GS+  E  T         F  CG C  AM+L+K S G  LL        + LP      +      CP+C F V+ +   ++    GY VCP CFN P  P
Sbjct:    4 LNVAEKPSVAKEIAAILSNGSSQRRPGFSQYNGIFEFP-FEMNNQRVQMVVTSVTGHLMALDFASNY-RSWKSCDPVELFSAAVEKTVREDSTQKQIEKTLKSEASKAQLLVLWLDCDREGENIAFEVKSVCEKVNRRLRVLRARFSALIPRDIHHAIHNLVQPNENLALACDARSEIDLRLGAAFTRFQTMRIQKKFPAVQNTSESRVVSFGSCQFPTLGFVVDRYLAIESFVREPFYYLHVTHTLQDVGS------IVFTWSRTRVYDHLACLALYETCLDAQIATITSISRRDTSKRKPFPLTTVEFQKRAARWLRISSEQAMTAAEALYNRGLLSYPRTETDSFKEGTDLMALLALHTEHETWGGYV-SLLQNGKYEHPRSGKNDDQAHPPIHPTKSVSLSSISNDVERKVYEFVVLHFLACCSMDARGSQTNVTMQIAQETFSASGLMIRERNYLDIYR-YEKWAATVIPVYERGDQFVPTIMELKFGETSPPPLLTESDLIAKMDQHGIGTDATIAEHINTILKRQYAIKMNNNTQFKPTELGLALVKSYDRMGYQLAQPDLRAAMERDCNAIAQGQKTCNQVVQSCMEQMRRIFESVVEKSRVLDETFQSYF---GSAKPEVLTANSRVVNPHFGMCGKCNKAMELRKLSDGGGLLTCAQCKGQYRLPIHATAVQTSTH--CPICRFIVVEIQKSDN--SPGYMVCPFCFNEPPNP 709          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: L1K3I3_GUITC (DNA topoisomerase n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1K3I3_GUITC)

HSP 1 Score: 629 bits (1622), Expect = 1.180e-215
Identity = 329/648 (50.77%), Postives = 441/648 (68.06%), Query Frame = 0

Query:    9 VAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALF--TAPLIRYVKEDKKDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRRNRNIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQEGVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVGELLDDGGFLWPREGRGDDGAHPPIHPTTSVDPNSLQEDEKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVKNEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSG 654
            VAEKPSVAKELA IL  G+++     SQYN  FEF    + G+   + +TSVTGHLME +F E + +KW  C+PI L   +  +I+ + +DKK LE+ L+EE R C  LV +LDCDREGENI+YEVIE+C + N  IR+ RA FSALIPRDI  +++ L  PN+  ++AVEAR EIDLRLG+AFTRFQTM L  RF++L + ++S+GPCQF TLGF+VDR  +I+ F PE FWS +   + D      GK  A F W+RGRL+D   C ++YEM +ED +A V SV  R   + RP+P+STV LQ  ASR   +SSE TM +AE LY +G ISYPRTETEKFK   ++  L+    ++  +GNY  ELL  G F WPR G+ DD AHPPIHPT     NSL   + ++YELV +HFLACCSK+A+G +T V  + G E F + GLM+ E NWL +Y P+ERW+ ++IP F +G++F P+ L M EG TQAPPLLSE+DLI LMD  GIGTDATIAEHI K+QQR Y +K++  +F PT+LG +LV  Y++IG +++ P  RA ME D T+I+ G+ +   V+E  +  MK  F+ C+A   ++   +++ F+PL +S   S+T++  R  S+CGVC   MDLKK  +G
Sbjct:    9 VAEKPSVAKELAAILSQGHAQAFNTHSQYNRKFEFS-YELDGRPCTLVVTSVTGHLMERDFDESH-RKWHTCEPIQLLDPSIQVIKMIPQDKKGLERNLLEEGRRCGQLVCFLDCDREGENISYEVIEICQKANPRIRVRRAHFSALIPRDIHRAMRTLTLPNQSLSEAVEARSEIDLRLGAAFTRFQTMVLGKRFEQLSDKLLSWGPCQFATLGFIVDRAWKIERFVPEDFWSFRCNLSRD------GKT-AHFDWRRGRLFDRVACTLIYEMLMEDPIATVNSVHERLKERWRPLPLSTVNLQTIASRKLRMSSEETMQIAEELYNKGFISYPRTETEKFKEGTNLQELISKQCESGHWGNYANELLA-GSFQWPRAGQNDDQAHPPIHPTQFT--NSLSGKQAQLYELVVRHFLACCSKNAIGAETTVEISIGVEQFVSKGLMIKELNWLKVY-PYERWSDKDIPIFTEGEQFEPTELAMTEGRTQAPPLLSEADLISLMDSHGIGTDATIAEHITKVQQRDYAIKSDSQQFSPTSLGKSLVTSYEAIGLELARPHFRARMEQDMTKIASGQTSARVVIEEWMEIMKPIFQRCIAGQTQMAQEMSVFFQPLNASSW-SQTLQ--RNVSQCGVCSGLMDLKKQRNG 640          

BLAST of mRNA_P-lacustris_contig92.15647.1 vs. uniprot
Match: A0A5D6XRA3_9STRA (DNA topoisomerase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XRA3_9STRA)

HSP 1 Score: 634 bits (1635), Expect = 1.770e-213
Identity = 339/627 (54.07%), Postives = 433/627 (69.06%), Query Frame = 0

Query:    1 MAVITAINVAEKPSVAKELANILGGGNSRRRQGRSQYNPIFEFENCAMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIALFTAPLIRYVKEDK--KDLEKQLVEEARGCQWLVLWLDCDREGENIAYEVIEVCTRR-NRNIRIFRARFSALIPRDILNSVQNLVPPNEHQAKAVEARQEIDLRLGSAFTRFQTMRLQNRFDELQE---GVISYGPCQFPTLGFVVDRYKRIQAFQPEAFWSIKMEYAEDDPASPGGKARADFAWKRGRLYDHACCAVLYEMTVEDGVAVVTSVEARRITKARPVPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINALLGDFRDNPRFGNYVGELLDDGGFLW--PREGRGDDGAHPPIHPTTSVDPNSLQED-EKKIYELVTKHFLACCSKDAVGHQTKVHANCGGEDFTATGLMVTERNWLVIYEPFERWTGRNIPEFFQGQEFAPSVLVMDEGMTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYCVK-NEQDRFFPTTLGLALVDGYDSIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISNMKQCFETCMANAHKLDDAVAMHF 617
            MAV   +NVAEKPSVAKE++ IL GG ++RRQG SQYNP++EF    + GQ V+M ITSV+GHLMELEF   Y + W +C P+ LFTA + +  + D+  + +EK LV EAR  QWLVLWLDCDREGENIA+EV  VC R  +R +R+FRARFSALIPRDI+++ Q+L  PNE  A A +AR EIDLR+G+AFTRFQTMRLQ+RF  L++   GVISYGPCQFPTLGFVV+R+  +Q F+ E F+ + + + + D   PG    A FAW RGR++D   C  LYE  V    A V +VE R   K +P+P++TVELQKRASR+  +SSE+TM  AE+LY +G++SYPRTET KFK   D+ AL+     +P++G+YV + L  G   +  PR G  DD AHPPIHPT SV   S+ +  E+K+YE VT+HFLACCSKDAVGHQT V     GE F+ +GLM+ ERN+L IY+ +E W+   IP + QG  F PS L M  G T  PPLLSE+DLI LMD  GIGTDATIAEHI+ I +R+Y  K N+  +F PTTLGLALV  Y+ +G  ++ P LRAAME DC  I+ G+     VV+ C+  MK+ F +   NA  LD   + HF
Sbjct:    1 MAVTKVLNVAEKPSVAKEISAILSGGQAQRRQGFSQYNPVYEFP-YELQGQRVHMTITSVSGHLMELEFDANY-RSWHSCSPVELFTAAVSKKTRADETQRKIEKTLVNEARQSQWLVLWLDCDREGENIAFEVKSVCERAASRRLRVFRARFSALIPRDIVHAAQHLAAPNEQLALACDARSEIDLRIGAAFTRFQTMRLQSRFPALKKDRGGVISYGPCQFPTLGFVVERFLHVQNFEREPFFRLDLAHRQAD--QPGAPTTA-FAWDRGRVFDRVACLCLYEALVAQPAARVEAVEKRPTWKRKPLPLTTVELQKRASRWLRVSSEQTMKAAESLYNKGVLSYPRTETNKFKEGTDVLALVRLHESSPQWGDYVSQTLLGGASKYEPPRHGGADDQAHPPIHPTKSVPLASIADAVERKVYEFVTRHFLACCSKDAVGHQTTVRVRIAGEGFSTSGLMIAERNYLDIYK-YENWSASAIPVYEQGDVFVPSALAMAAGETAPPPLLSEADLIALMDSNGIGTDATIAEHIKTILKREYAEKVNDGTQFKPTTLGLALVLSYEHMGLDLAKPALRAAMENDCKAIALGQKDCRQVVQTCMEQMKEIFLSVTRNAQVLDQTFSEHF 621