prot_P-lacustris_contig911.15575.1 (polypeptide) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: D7FUT0_ECTSI (Methionine synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUT0_ECTSI)

HSP 1 Score: 2241 bits (5808), Expect = 0.000e+0
Identity = 1113/1223 (91.01%), Postives = 1168/1223 (95.50%), Query Frame = 0

Query:   67 DYHVLVKGNNDMLSITQPQIIKKIHAEYLEAGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKALRNKGKATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLGDRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPAATVGGEDVEVYANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            DYH+LVKGNNDMLSITQP IIK+IH +Y+++GSDII TNTFS+TTIAMADYEMEKDVYELNFESAR+AREACEEATAKDPT PRFVMG+LGPTNRTGSISPDVEDPSFRNVTFDELV AYKDQTK LIDGG+D+IIVETIFDTLNAKA VFAVREYFDEQG+TDVPLFVSGTLVDQSGRTLSGQTTEGFY SMRHCKPFC+GLNCALG +QMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYD+TP+DMARDNLLF +EGL+NMLGGCCGSTPAHIKA   A   + C  R LP PREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGE+EF+RHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYD+LVDEVGFPPEDIIFDPNILTIATGI+EHNNYAVDFIQATERIK ICPYCKISGGVSNLSFGFRGVN IRESMHSVFL+HAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNN+H GATEALLDAAEIE+EKAL+NKGK T E +GWRTQGV+ RLTHSLIKGIDQ+VIEDVEECRL+LD QGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDP S+++EDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCD ILA+A +L VD+IGLSGLITPSLDEMVFVAKEMAK G KLPLLIGGATTSKMHTAVKISPQYASPETPV+HVLDASRSVTVVGALL DRK DYVEE+MEEYEELREEHYAGQEERHYL FEKAS K K IDF KRPPVP P KPGITVVT +Y LDDVLDYIDWNPFFQTWELRGRYPNRGYPKIF DESVGEEAKKLF +AQ ML KIVKEK+L+L G+V IFPAATV GEDVEVYAN+SREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESG+AD+MGMFA+ACMGC++MVKEFEDLNDDYSKIMVQAL DRFVEAFAEKLHED+RK IWGYAP+EKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWD+LNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMD+ETCEKWLSPIL+YDRD
Sbjct:    4 DYHILVKGNNDMLSITQPHIIKEIHTKYVDSGSDIIATNTFSATTIAMADYEMEKDVYELNFESARLAREACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGADIIIVETIFDTLNAKAGVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAEEGLINMLGGCCGSTPAHIKAMKDAV--EPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQERLTHSLIKGIDQWVIEDVEECRLMLDDQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDLILAKAKQLKVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKNIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVAIFPAATVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAIACMGCEKMVKEFEDLNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1224          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A7S2XWE9_9STRA (Methionine synthase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XWE9_9STRA)

HSP 1 Score: 1875 bits (4858), Expect = 0.000e+0
Identity = 947/1279 (74.04%), Postives = 1079/1279 (84.36%), Query Frame = 0

Query:   12 GSVANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRYKLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLEAG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKALRNKGKATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLGDRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPAATVGGEDVEVYANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            G+V NYPYF R KDS  AYL RLFQE I ++DGAMGTMIQ+YKLGEEEYRGER+ DYH LVKGNNDMLSITQPQIIK+IH +YLE G S +IGTNTFSSTTIAMADY+ME  V ELN ES R+AREAC+E TAKDPT PRFV+G++GPTNRTGSISP+VEDPS RNVTFDEL  AY +Q   L++GG D+++VETIFDTLNAKAAVFA   + +E GL D+PLFVSGTLVDQSGRTLSGQTTE FY S+RHCKPFC+GLNCALG TQM+PFL+RLA VAECFVHVYSNAGLPNAMGGYDDTP DMAR+NL+F DE LVNM+GGCCGSTP HIKA + A   K   PRPLP  +EP +WLSGLEDL+V+K+RF FLNVGERCN++GSIRFKKLIL GDY TAMD+AK+QVEDGAMV+DIN+DDG+LDG+AAMQKF KIAVTEP++SKVPFMIDSSKF IVEAG+KWVQGKSI+NSISLKVGE+EF+R A I+K HGAAV+VMAFDEEGQAA    KVRICKRSYDILV+EV FPPEDIIFDPNILTIATGIEEHNNY VDFI+AT++IK ICPY +ISGGVSNLSFGFRGVN IRE++HSVFLYHAV AGMDMGIVNAGM+EVYDDI   LL++VEDAV N+H  ATE LL+ +  E+EKA    G    E   WR + V  RLT++LIKGI Q++ EDVEECR  +D    RPL VIEGPLM GMN+VGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFME EK A + A GI  + + E+DDS YAGKVL+ATVKGDVHDIGKNIV+VVLGCNNYKV+D+GVMQPC+ IL    +   D++GLSGLITPSLDEMVFVAKEM KAG+K PL+IGGATTSKMHTAVKISPQYA+ E PV+HVLDASRSVTVV +LLGD K ++VE+LMEEYE+LREEHYAG EER +L+ ++A  KG +IDF   PPV AP + G+TV+ D+Y L+DVL YIDWNPFFQTWELRGRYPNRGYPKIF DE+VG EAKKLF++AQ ML  IV  K L L G+ GIF A  VG EDVEVY    + EV A+FCMLRQQAEKES+ PYLS ADFIAPK+SGL D+MGMFAVACMG ++    FE  NDDYSKIMVQALADR VEAFAE LH D+R +IWGYAP+EK+  +DL K++YQGIRPAPGYPSQPDHTEK TMW++L   E +GI LSE+LSM+PASSVSAL FAHP +EYFAVG I KDQV  YAERKGM+V   EKWLSPILNYDR+
Sbjct:   11 GTVENYPYFNREKDSVRAYLTRLFQEEIAIYDGAMGTMIQKYKLGEEEYRGERFKDYHTLVKGNNDMLSITQPQIIKEIHLKYLEEGNSQLIGTNTFSSTTIAMADYDMESYVRELNVESVRLAREACDEVTAKDPTRPRFVVGAIGPTNRTGSISPNVEDPSVRNVTFDELEIAYYEQVCGLVEGGCDILMVETIFDTLNAKAAVFACNRFLEEHGLEDMPLFVSGTLVDQSGRTLSGQTTEAFYVSIRHCKPFCVGLNCALGATQMKPFLRRLAVVAECFVHVYSNAGLPNAMGGYDDTPDDMARENLIFADEKLVNMIGGCCGSTPPHIKAISEAV--KGMPPRPLPEKKEPVLWLSGLEDLVVSKDRFTFLNVGERCNIAGSIRFKKLILGGDYATAMDIAKKQVEDGAMVLDINVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGMKWVQGKSIINSISLKVGEEEFKRQAGIIKRHGAAVIVMAFDEEGQAATESEKVRICKRSYDILVNEVQFPPEDIIFDPNILTIATGIEEHNNYGVDFIEATKKIKEICPYARISGGVSNLSFGFRGVNVIREAIHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPKDLLQLVEDAVLNRHSEATEKLLERSLEEKEKAASGGGPKKKEVE-WRIKPVTERLTYALIKGISQYIDEDVEECRQTVD----RPLQVIEGPLMQGMNIVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKKAALEAKGI--TEMPEDDDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMQPCESILDAIKKYQPDVVGLSGLITPSLDEMVFVAKEMEKAGLKQPLMIGGATTSKMHTAVKISPQYANNEHPVIHVLDASRSVTVVQSLLGDDKDEFVEDLMEEYEDLREEHYAGLEERCFLALDQAKQKGLQIDFAACPPVQAPNQLGVTVL-DSYTLEDVLPYIDWNPFFQTWELRGRYPNRGYPKIFNDENVGGEAKKLFDEAQEMLKDIVANKRLHLKGVCGIFAANRVG-EDVEVYTGADKSEVAAKFCMLRQQAEKESEAPYLSMADFIAPKDSGLDDYMGMFAVACMGAEEEAARFEAENDDYSKIMVQALADRLVEAFAEALHRDMRVSIWGYAPDEKLPAEDLHKIRYQGIRPAPGYPSQPDHTEKTTMWNLLQVEEKSGIRLSEALSMLPASSVSALCFAHPQAEYFAVGKIEKDQVVEYAERKGMEVAEMEKWLSPILNYDRE 1278          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A836CPG7_9STRA (Methionine synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CPG7_9STRA)

HSP 1 Score: 1851 bits (4795), Expect = 0.000e+0
Identity = 947/1281 (73.93%), Postives = 1061/1281 (82.83%), Query Frame = 0

Query:   13 SVANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRYKLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLEAGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREP-FMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKALRNK--GKATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLGDRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGI--TVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPAATVGGEDVEVYANESREEVLARFCMLRQQAEKESD-DPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYD 1287
            SVA+YP + +GKDS  AYL RLFQERIVM+DGAMGTMIQ+++L EE+YRGER+ DY  LV+GNND+LSITQPQII++IH EYLE+GSDIIGTNTFSSTTIA ADY ME  VYELN+ESA++AR AC+E TA+DP+ PRFV GS+GPTNRT SISP VEDPS+RNV FDELV AY  + KAL++GGSD+IIVETIFDTLNAKAAVFAVR+Y  E    D+PL +SGTLVDQSGRTLSGQT EGFY SMRH KPFC+GLNCALG  QM+PFL+RL+ VAECFVHVYSNAGLPNAMGGYDDTP DMARDN  F  EG+VNM+GGCCGSTP HIKA +     K C PRPLP P +P  MWLSGLE L V+K+R  FLNVGERCN SGSI+FKKLI+ G+Y +AMDVAK QVEDGAMV+DINLDDGLLDG+AAMQKFCKIAVTEPDVSKVPFMIDSSKF I+EAGLKWVQGK IVNSISLKVGE EFRR A IV+ HGAAVVVMAFDEEGQAAD ++KVRICKRSYDILVDE+ FPPEDIIFDPNILTIATGI+EHNNYA+DFI AT RIK +CP+CKISGGVSNLSFGFRGVN IRE+MHSVFLYHA++AGMDMGIVNAGML+VYDDI P LLKIVEDAV NKHPGATEALL+ A+IERE A   K  GK   +   WR Q V+ARLTHSLIKGIDQ++  D EE R    +Q  RPL VIEGPLM GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFME EK  K +  G+DP+   + DD+ YAGKV+IATVKGDVHDIGKNIVAVVLGCNNYKVYD+GVM  C+ IL    +   DI+GLSGLITPSLDEMVFVA+EMAK G   PL+IGGATTSKMHTAVKI+PQY+SPE PV+HVLDASRSVTVV +LLG  K +Y EE++EEYEELRE            + EKA  K  +I+F      PAP  P      V D Y+L+ VLDYIDWNPFFQTWELRGRYPNRGYPKIF D +VG EAKKLF+DAQ MLN IV  K L LTG++G++PA  VG EDV +Y++ SR E LA FCMLRQQAEK++  + YLS ADF+AP   G  DH+GMFAVACMGCD  VK FE  NDDYSKIMVQALADR VEAFAE LH D+R+ IWG+A  EKV G+DLFKLKYQGIRPAPGYPSQPDHTEKRTMW+VL A EL GI+LSESLSMMPASSVSALVFAHP SEYFAVG I KDQV SYA RKGM ++ C++WL PILNYD
Sbjct:   14 SVADYPRYTKGKDSCTAYLTRLFQERIVMYDGAMGTMIQKHRLEEEDYRGERFKDYDKLVRGNNDLLSITQPQIIRQIHIEYLESGSDIIGTNTFSSTTIAQADYGMENLVYELNYESAKLARSACDEVTARDPSRPRFVAGSIGPTNRTASISPSVEDPSYRNVNFDELVDAYYFEAKALVEGGSDIIIVETIFDTLNAKAAVFAVRKYLRETNQEDIPLMISGTLVDQSGRTLSGQTGEGFYVSMRHVKPFCIGLNCALGAQQMKPFLRRLSMVAECFVHVYSNAGLPNAMGGYDDTPSDMARDNHEFASEGMVNMIGGCCGSTPQHIKAMH--EDVKDCAPRPLPKPLDPPVMWLSGLEPLQVSKDRINFLNVGERCNCSGSIQFKKLIMAGNYSSAMDVAKAQVEDGAMVVDINLDDGLLDGLAAMQKFCKIAVTEPDVSKVPFMIDSSKFEILEAGLKWVQGKCIVNSISLKVGETEFRRQATIVRDHGAAVVVMAFDEEGQAADEESKVRICKRSYDILVDELKFPPEDIIFDPNILTIATGIDEHNNYAIDFINATRRIKEVCPHCKISGGVSNLSFGFRGVNTIREAMHSVFLYHAIQAGMDMGIVNAGMLQVYDDIAPDLLKIVEDAVLNKHPGATEALLERAQIERELADARKAGGKVAVKEGSWRDQPVKARLTHSLIKGIDQYIETDTEEAR----SQADRPLSVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKRLKALEQGVDPNEPVD-DDAAYAGKVVIATVKGDVHDIGKNIVAVVLGCNNYKVYDLGVMVSCEDILKAVDKYKPDIVGLSGLITPSLDEMVFVAREMAKKGCTQPLMIGGATTSKMHTAVKIAPQYSSPEHPVIHVLDASRSVTVVSSLLGGEKTEYAEEVLEEYEELREXXXXXXXXXXXXTLEKAMGKRLEINFEA---TPAPQPPSFIGNKVLDNYSLESVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDATVGAEAKKLFDDAQVMLNDIVANKRLRLTGVLGMYPANRVG-EDVVLYSDASRAEKLATFCMLRQQAEKDTPTEAYLSLADFVAPV--GHRDHLGMFAVACMGCDDEVKRFEAENDDYSKIMVQALADRLVEAFAEALHADVRREIWGFASEEKVAGEDLFKLKYQGIRPAPGYPSQPDHTEKRTMWNVLKAEELAGIQLSESLSMMPASSVSALVFAHPQSEYFAVGKIGKDQVESYAARKGMPLDECQRWLGPILNYD 1281          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A7S4DBJ3_HETAK (Methionine synthase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBJ3_HETAK)

HSP 1 Score: 1847 bits (4783), Expect = 0.000e+0
Identity = 939/1286 (73.02%), Postives = 1063/1286 (82.66%), Query Frame = 0

Query:    9 EPKGSVANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRYKLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLEAG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKALRNKGKATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLG-DRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPAA-TVGGEDVEVYANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGLA--DHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            E KGSV NYPYF R  DS  AYL RLFQE+IVM+DGAMGTMIQ+YKL EE+YRGE++ D+H LVKGNNDMLS+TQP+IIK+IH +YL  G S +IGTNTFS TTIAMADYEME  V ++NF SARIARE  +E TA DPT PRFV+G++GPTNRTGSISP VED S RNVTFDEL AAY  QT  L++GGSD+IIVETIFDTLNAKAA+FAV  YF+E G T+ PLF+SGTLVDQSGRTLSGQT E FYAS+RH KPFC+GLNCALG  QM+PFL+RLA V ECFVHVYSNAGLPNAMGGYDDTP+DMAR+N +F  E LVNM+GGCCGSTP HI A   A   +   PR LP   EP MWLSGLEDLIV+K+RF FLNVGERCN++GSI FKK I+ GDY  AM++AK+QVEDGAMVID+N+DDG+LDG+AAMQKF KIAVTEP++SKVPFMIDSSKF IVEAGLKWVQGKSIVNSISLKVGE+EF+RHA I K HG A+VVMAFDE GQAA  D+KV +CKRSYDILVDEVGFPPEDIIFDPNILT+ATG+EEHNNY VDFI AT RIK  CP+ KISGGVSNLSFGFRGVN IRE+MHSVFLYHAV AGMDMGIVNAGM+EVYDDI   LL +VEDAV N+ P ATE LLD + +EREK     GK   +   WRT+ V  RLTH+LIKGID+F+  DVEECR  +     +PL+VIEGPLM+GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFMEAEK AK+ A G+DP   +E  DS YAGKVL+ATVKGDVHDIGKNIV+VVLGCNNYKV+D+GVM  C+ ILA+A E  VD+IGLSGLITPSLDEMVFVAKEMAK G+K PL+IGGATTSKMHTAVKI+PQYA+ E PV+HVLDASRSVTVV +LL   +K  YVEEL+EEYEELREEHYA  EER YL F K   KG ++DF  RPPV AP  PG+  + + Y L DV+ +IDWNPFFQTWELRGRYPNRGYPKIF DE+VG EA+KLF+DAQAML  IV  K L LTG+  IFPAA T  GEDVEV++ E     L +FCMLRQQAEKE+D+PYLS ADF+AP+  G A  DH+G FAVAC+G D+     E  NDDY+KIMVQALADR VEAFAEKLH DIR  +WGYAP+E++  +DL K++YQGIRPAPGYPSQPDHTEKRTMWD+L   EL+GI LSESLSMMPASSVSALVFAHPD++YFAVG I +DQV  YA RKGMDV   EKWLSPILNYDRD
Sbjct:   11 ETKGSVENYPYFNRETDSVKAYLCRLFQEKIVMYDGAMGTMIQKYKLSEEDYRGEQFKDFHKLVKGNNDMLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAAI--AADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGKVV-KAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAV----AKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEAEKAAKLAAQGLDPQEADE--DSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQAL-EGYELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGHSSLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEAARHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 1286          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A097PA47_9STRA (Methionine synthase n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A097PA47_9STRA)

HSP 1 Score: 1822 bits (4720), Expect = 0.000e+0
Identity = 915/1279 (71.54%), Postives = 1067/1279 (83.42%), Query Frame = 0

Query:   16 NYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRYKLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLEAGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTD-VPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKALRNKGKATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLGDRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPAATVGGEDVEVYANESR----EEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            +YP F RG+DST+AY ++LF+ERIV++DGAMGTMIQ+++L EE+YRGER+ DYH LV+GNND+L++TQP IIK IH +YL+ G+D+IGTNTFSSTTIA ADY+ME   YELN ESARIARE  +E +A++P  PRFV GS+GPTNRT SISPDV D S RNVTFDEL  AY ++ + LIDGG D II+ETIFDTLNAKAA+F+VR    E+G+ + +PL +SGTLVDQSGRTLSGQTTE FY S+RHC PFC+GLNCALG  QMRPFLQRL+KVAECFV VYSNAGLPNAMGGYDDTP DMA DN +F  E LVNM+GGCCGSTP HI A   A  C+   PRP+   R   MWLSGLEDL+V+KERFQFLNVGERCN++GS  FKKLIL GDY  AMD+AK+QVEDGAMV+D+N+D+GLLDG+AAMQ+F KIA+TEP+ SKVP M+DSSKF I+EAGLKWVQGK IVNSISLKVGE+EF+R A +VKAHGAAVVVMAFDEEGQAAD  NKVRICKRSYDILVDEVGFPPEDI+FDPNILTIATGIEEHNNYAVDFI+A   IK +CP+ KISGGVSNLSFGFRGVN IRE+MHSVFLYHAV+AGMDMGIVNAGML+VY DI P LL+IVEDAV NKH  ATEALL+ + +EREKAL NKG+ATA    WRT  V+ARLTH+L+KGID+F+ EDVEECR L+     RPL+VIEGPLMSGMNVVGDLFG+GKMFLPQVIKSARVMKKAVA+LLPFME EK  KM   GI+  +   +DDS YAG VL+ATVKGDVHDIGKNIV VVLGCNNYKVYDMGVM PC++I+ +A+E+   +IGLSGLITPSLDEMVFVAKEM+KAG+ +PLLIGGATTSKMHTAVKI+P Y++ E P +HVLDASRSVTVVG+LLG+ K +YV+++++EY+ELREEHYAG EERH  +F+ A   G  IDF   PPV +P+      V D Y+L+DV+ +IDWNPFFQTWELRG+YPNRGYP+IF DE+VGEEAKKLF DAQA+L  +V  K LSL G+VG+FPAA VG EDVEVYA+E      EE + +FCMLRQQ EKESDD Y S ADF+A KESG  D++G FAVAC G ++ V+ +E LNDDY+KIMVQALADRFVEAFAE LH ++R   WGYAP E+++ +DL K+KY GIRPAPGYPSQPDHTEK  MW++L A EL GI L+ESLSM PASSVSALVFAHP+S+YFAVG I KDQV  YA RKGM VE  E+WL+PILNYDRD
Sbjct:   13 DYPRFVRGRDSTLAYFHKLFRERIVIYDGAMGTMIQKHRLTEEDYRGERFKDYHKLVRGNNDLLTLTQPTIIKDIHRKYLKGGADLIGTNTFSSTTIAQADYDMESLAYELNVESARIAREVADEVSAEEPERPRFVGGSIGPTNRTASISPDVVDASKRNVTFDELDEAYYEEARGLIDGGCDFIIIETIFDTLNAKAAIFSVRRLLKERGMEETMPLIISGTLVDQSGRTLSGQTTEAFYVSVRHCNPFCVGLNCALGAKQMRPFLQRLSKVAECFVSVYSNAGLPNAMGGYDDTPADMAADNSVFVKESLVNMIGGCCGSTPDHINAI--AKMCEGVPPRPVH-ERVKHMWLSGLEDLVVSKERFQFLNVGERCNIAGSPLFKKLILAGDYAKAMDIAKKQVEDGAMVLDVNMDEGLLDGLAAMQRFLKIAMTEPECSKVPIMVDSSKFEIIEAGLKWVQGKCIVNSISLKVGEEEFKRQAAVVKAHGAAVVVMAFDEEGQAADEYNKVRICKRSYDILVDEVGFPPEDIVFDPNILTIATGIEEHNNYAVDFIKACRTIKEVCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVQAGMDMGIVNAGMLQVYSDIPPDLLQIVEDAVLNKHAEATEALLERSMVEREKALANKGQATAVQAPWRTLEVQARLTHALVKGIDEFIDEDVEECRQLVP----RPLEVIEGPLMSGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPFMEKEKEEKMKEQGIEAVA---DDDSAYAGVVLLATVKGDVHDIGKNIVGVVLGCNNYKVYDMGVMIPCEKIIEKAIEVKAQVIGLSGLITPSLDEMVFVAKEMSKAGMTVPLLIGGATTSKMHTAVKIAPNYSTVEHPCIHVLDASRSVTVVGSLLGEDKEEYVQDVLDEYDELREEHYAGLEERHLKTFDAAKKLGFNIDFGASPPVCSPVGGTGVKVVDYYSLEDVVPFIDWNPFFQTWELRGKYPNRGYPRIFNDENVGEEAKKLFADAQALLKDLVDNKRLSLRGVVGVFPAARVG-EDVEVYASEEAKDRGEEAIGKFCMLRQQQEKESDDAYFSLADFVASKESGHKDYLGAFAVACFGAEEEVERYEALNDDYNKIMVQALADRFVEAFAEALHREMRMKTWGYAPEEELDPEDLLKIKYDGIRPAPGYPSQPDHTEKVMMWELLKARELAGIGLTESLSMTPASSVSALVFAHPESQYFAVGRIEKDQVEDYAVRKGMVVEEVERWLAPILNYDRD 1280          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A7S4J684_9STRA (Methionine synthase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4J684_9STRA)

HSP 1 Score: 1699 bits (4399), Expect = 0.000e+0
Identity = 860/1291 (66.62%), Postives = 1029/1291 (79.71%), Query Frame = 0

Query:   16 NYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRY----KLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLE-AGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAV-KAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKALRNK---GKATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLGDRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPA-ATVGGEDVEVYANESREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            ++P+F RG+DS   Y+ +LF  RI  +DGAMGTMIQ +    KL EEEYRGE + D+   VKGNNDMLS+T+P +I  I+ +YLE  GSD+IGTNTFSSTTIAMADYEME   YELN+E AR+AR AC+E TA DP+ PRFV+G++GPTNRTGSISP VEDPS RNVTFDELV  Y +Q   L+DGGSD+++VETIFDTLNAKAA++AV EY +  GL D+P+FVSGTLVDQSGRTLSGQT E FYAS+RH KP C+GLNCALG   M PF+++LAK  ECF+HVYSNAGLPNAMGGYD+TP+ MA  N +F ++G +NM+GGCCGSTP HIKA       ++  PRPLP    P MWLSGLEDL+V          FLNVGERCN++GSI+FKKL++KGDY +AMD+AK QVEDGA VIDIN+DDG+LDG+AAMQKF KIAVTEP+V KVPFM+D+SKF IV AGLKW QGK IVNSISLKVGE  F+  A ++K HGAAVVVMAFDE GQAA+ D KVRICKRSYD+LVDEVGFPPEDIIFDPN+LTI TG+EEHN+Y +DFI AT+RIK  CPY KISGG+SNLSFGFRGV  IRES+H+VFL+HA+ ++GMD+GIVNA  +    ++EP L    E+ V NK   +T+ +LD  + E+E     K   G    + T WR      RL HSL+ GI +FV++DVEE R   D    +PLDVIEGPLM GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFM+ EK   MIA G++P   +E D+S +AGKVL+ATVKGDVHDIGKNIV+VVLGCNNYKVYD+GVM  C++IL +A E +VD+IGLSGLITPSLDEMV VAKEM+KAG K PLLIGGATTSKMHTAVK+SP Y + E PV+HVLDASRSVTVV +LLG  K D+V ++MEEY+E+RE++YAG E+R++L+F++A  +   IDF  +PP PAP K GITV+ D+ +L+D++ YIDWNPFFQTWELRGRYPNRGYPKIF DE+VG+EAKKLF+DA AM+N  + EK++ L G+VG+FPA  +  GEDV VY +E+        A FCMLRQQAEKE+DDPYLSQADF+AP  +G  DH+GMFAV+C GCD +V +FE  NDDYSKIM QALADRFVEAFAE LH  IR  +WGYAP+E+++  DL K+KY GIRPAPGYPSQPDHTEKRTMWDV+ A EL GI+LSESLSMMPA+SVSALVFAHPDS+YFAVG I KDQV SY  RK  ++   E+WLSPILNY+RD
Sbjct:  138 SFPHFVRGRDSAREYITKLFTSRIAFYDGAMGTMIQNFSKKNKLEEEEYRGEHFKDWKCNVKGNNDMLSMTRPDVISGIYKQYLEIGGSDMIGTNTFSSTTIAMADYEMEDYAYELNYEGARLARLACDEVTAADPSRPRFVVGAIGPTNRTGSISPSVEDPSVRNVTFDELVETYFEQAVGLVDGGSDILMVETIFDTLNAKAALYAVGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAQHMVPFVEKLAKCVECFMHVYSNAGLPNAMGGYDETPEMMAEYNKVFFEKGWLNMVGGCCGSTPPHIKAIRECA--QNFSPRPLPAVGRPKMWLSGLEDLVVDDVHNHLGLPFLNVGERCNIAGSIKFKKLMMKGDYGSAMDIAKAQVEDGAHVIDINVDDGMLDGLAAMQKFVKIAVTEPEVCKVPFMLDASKFEIVMAGLKWCQGKPIVNSISLKVGEKLFKEQATLLKKHGAAVVVMAFDENGQAAEEDEKVRICKRSYDMLVDEVGFPPEDIIFDPNVLTIGTGMEEHNDYGIDFINATKRIKEQCPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAILESGMDVGIVNAKEMLALSEVEPVLKTACENLVFNKTADSTDVMLDLTKKEKEAIEARKRGGGAVEVKKTSWRDLEATKRLEHSLVNGISEFVVKDVEEARQKCD----KPLDVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMDKEKKEAMIAKGLNPDDFDENDESNFAGKVLMATVKGDVHDIGKNIVSVVLGCNNYKVYDIGVMVSCEKILEKAKEYDVDVIGLSGLITPSLDEMVVVAKEMSKAGFKQPLLIGGATTSKMHTAVKLSPNYFTLEHPVIHVLDASRSVTVVSSLLGRDKGDFVTDIMEEYDEMREDYYAGLEDRYFLTFDQAKEQKCVIDFESKPPAPAPKKLGITVI-DSVSLEDIVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGKEAKKLFDDAIAMMNTFISEKSMHLKGVVGLFPANRSEDGEDVHVYLDEAARAAGTPEATFCMLRQQAEKENDDPYLSQADFVAP--AGYKDHLGMFAVSCFGCDDLVNKFEAENDDYSKIMSQALADRFVEAFAEYLHRGIRTEMWGYAPDEELDEADLLKIKYDGIRPAPGYPSQPDHTEKRTMWDVIKAEELAGIQLSESLSMMPAASVSALVFAHPDSQYFAVGQIGKDQVESYGARKKFELSVMERWLSPILNYERD 1418          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A7S1ZPU5_9STRA (Methionine synthase n=3 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZPU5_9STRA)

HSP 1 Score: 1694 bits (4386), Expect = 0.000e+0
Identity = 867/1291 (67.16%), Postives = 1032/1291 (79.94%), Query Frame = 0

Query:   16 NYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRY----KLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLEAG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAV-KAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKA-LRNKGKATAETT--GWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLGDRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPA-ATVGGEDVEVYANESREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            +YP+F RG+DS   Y+  LF  +I ++DGAMGTMIQ Y    KL EEEYRG+R+ D++  VKGNNDMLSITQP +I  I+ EYLE G S++IGTNTFSSTTIAMADY ME   YELN+E AR+AR+AC+E TA DPT+PRFV+G++GPTNRTGSISP VEDPS RNVTFDELV AY +Q   L+DGG+D+++VETIFDTLNAKAA++AV EY +  GL D+P+FVSGTLVDQSGRTLSGQT E FYAS+RH KP C+GLNCALG   M PF+++LAKV ECF+HVYSNAGLPNAMGGYD++P DMAR+N  F + G +NM+GGCCGSTP HIKA       ++  PR LP    P MWLSGLEDL+V          FLNVGERCN+SGSIRFKKL++ GDY TAMD+AK+QVEDGA V+DIN+DDG+LDG+AAMQKF KIAVTEP+VSKVPFM+D+SKF IV AGLKW QGK IVNSISLKVGE+ F+  A ++K HGAAVVVMAFDEEGQAA  D KVRICKRSYDILV+EV FPPEDIIFDPN+LTI TG+EEH NY VDFI A + IK  CPY KISGG+SNLSFGFRGV  IRES+H+VFL+HA+ ++GMD+GIVNA  +    ++E  L K  E+ V NK P AT+A+L+  + E+     R KG A  E     WR    + RL HSL+ GI Q++ +DVEE RL+      +PLDVIEGPLM GMNVVGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFME EK   +I  G+DP   + EDDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNNYKVYD+GVM  C+ IL +A E NVD+IGLSGLITPSLDEMV VAKEM+KAG K PLLIGGATTSKMHTAVK+SP Y + E PV+HVLDASRSVTVV +LLG+ K D+V ++MEEY+E+RE++YAG E+R++L+FE+A  +  KIDF  + P PAP K GITVV D   L++V+ YIDWNPFFQTWELRGRYPNRGYPKIF DE+VG EA+KLF+DAQAML +I+ EK++ L G+VG+FPA  +  GEDV +Y++E         A FCMLRQQAEKE++DPYLSQADFIAP  +G  DH+GMFAV+C GC+ +V+++E  +DDYSKIM QALADRFVEAFAE LH+ IR  +WGYA NE++   DL K+KY GIRPAPGYPSQPDHTEK TMW+++ A EL GI LS+SLSMMPA+SVSALVFAHPDS+YFAVG + KDQV SYAERK  ++   E+WLSPILNY+RD
Sbjct:   82 SYPHFVRGRDSLREYITSLFTSKIAIYDGAMGTMIQNYSKRNKLDEEEYRGDRFKDWNCNVKGNNDMLSITQPHVISGIYKEYLEVGGSNLIGTNTFSSTTIAMADYNMEAYAYELNYEGARLARQACDEVTAADPTNPRFVVGAIGPTNRTGSISPSVEDPSARNVTFDELVEAYFEQIVGLMDGGADILMVETIFDTLNAKAALYAVGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAVHMIPFVEKLAKVVECFLHVYSNAGLPNAMGGYDESPDDMARNNKTFFENGWLNMVGGCCGSTPPHIKAIRECA--ENYAPRKLPDVGRPKMWLSGLEDLVVDDVHNHLGLPFLNVGERCNISGSIRFKKLMMAGDYATAMDIAKKQVEDGAHVLDINVDDGMLDGLAAMQKFVKIAVTEPEVSKVPFMLDASKFEIVLAGLKWCQGKCIVNSISLKVGEELFKEQATLLKKHGAAVVVMAFDEEGQAATEDEKVRICKRSYDILVNEVHFPPEDIIFDPNVLTIGTGMEEHANYGVDFINAVKTIKEQCPYVKISGGISNLSFGFRGVMKIRESIHAVFLHHAILESGMDVGIVNANEMLHVTEVEGELKKACENLVFNKTPDATDAMLELTQKEKRAIEARKKGGAVTEIKEKSWRDFEAKKRLEHSLVNGISQYIDKDVEEARLM----SSKPLDVIEGPLMDGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMEEEKKQALIDAGLDPEEFDPEDDSAYAGKVLMATVKGDVHDIGKNIVAVVLGCNNYKVYDIGVMVSCETILEKAKEYNVDVIGLSGLITPSLDEMVVVAKEMSKAGFKQPLLIGGATTSKMHTAVKLSPNYFTLEHPVIHVLDASRSVTVVSSLLGENKEDFVTDIMEEYDEMREDYYAGLEDRYFLTFEQAKEQKFKIDFDAKSPAPAPNKVGITVV-DYVELENVVPYIDWNPFFQTWELRGRYPNRGYPKIFDDEAVGNEARKLFDDAQAMLKEIIAEKSMHLKGVVGLFPANQSEDGEDVHIYSDEDSRAAGNPTATFCMLRQQAEKENEDPYLSQADFIAP--AGYKDHLGMFAVSCFGCEALVEKYEAEHDDYSKIMSQALADRFVEAFAEYLHKVIRTEMWGYANNEELNEADLLKIKYDGIRPAPGYPSQPDHTEKTTMWNLIKAKELAGISLSDSLSMMPAASVSALVFAHPDSKYFAVGQVGKDQVESYAERKKFELPVAERWLSPILNYERD 1362          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A7S3JTL8_9STRA (Methionine synthase n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JTL8_9STRA)

HSP 1 Score: 1691 bits (4380), Expect = 0.000e+0
Identity = 859/1299 (66.13%), Postives = 1034/1299 (79.60%), Query Frame = 0

Query:    9 EPKGSVAN-YPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRYKLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYL-EAGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDE-VGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHA-VKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKA-LRNKGK---ATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLG-DRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPAA-TVGGEDVEVY----ANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            +PKG     +PYF RG+DS   YL +LFQ RI M+DGAMGTMIQ++KL EE++RGER+ DYH+L+KGNND+LSIT+P II  I+ +YL E GS +IGTNTFSSTTIA ADY+M+  +YELN++SAR+ARE C++ TA+DPT PRFV+G++GPTNRT SISPDV DP+ RN TFDELV AY +Q   L+DGG+D++IVETIFDTLNAKAAVFAV EY +   + D+PL +SGTLVDQSGRTLSGQT E FYAS+RH KP C+GLNCALG + M PFL+RLA  AECFVHVYSNAGLPNAMGGYDDTPQDMAR+NL+F + G VNM+GGCCGSTP HI A   A   K C+PRPLP  + P MWLSGLED +V          F+NVGERCN++GS +FK+L+++G +  AMD+AK QVE+GA ++D+N+DDG+LDGM AMQKF K+AVTEPDVSKVPFMIDSSKF IVEAGLKW QGK IVNSISLKVGE++F+ HA  ++ HGAAVVVMAFDE+GQAA  ++KVRICKRSYDILV E + +PPEDI+FDPNILTI TGIEEHNNY VDFI A   IK +CP+ KISGGVSNLSFGFRGVN IRES+H+VFL+HA + +GMDMGIVNA  +    ++   L  +  DAV N  P ATE LL A E E+E A  R +G    A A+TT WR   +E R+THSL+KGI +FV +D EE R  + A+G RPL+VIEGPLM+GMNVVGDLFG+GKMFLPQVIKSARVMKKAVA+LLP+MEAEK   +IA GIDP S  ++DDSQYAGK LIATVKGDVHDIGKNIVAVVLGCNNYKVYD+GVM  CD+IL +A E  VD IGLSGLITPSLDEMVFVAKEM+K G+++PLLIGGATTSKMHTAVKI+PQYAS E PV+HVLDASR+VTVV +LL  D K DYV ++ +EY ELR+E+YA  E+R +L+ E+A  K  ++++ K PP P   K  +   T TY L DV+  IDWNPFFQTWELRGRYPNRGYPKIF DE+VG EAK+LF+DAQ M+ +IV EK L L G+ G++ A  + GGEDV+V+    A +++E+  A FCM RQQAE ES   Y+SQADFIAP+ +   D++GMFAVAC G ++   ++E  NDD+SKIM+QALADR VEA AE LH D+R   WGYA +E +  +DL K+KY+GIRPAPGYPSQPDHTEKRTMWD+L+    +GI+LSES+SMMPASSVSALVFAHP++ YFAVG I KDQV SYAERKG  +   E+WLSP+LNYDRD
Sbjct:   76 QPKGLYPEGFPYFVRGRDSVREYLTKLFQTRITMYDGAMGTMIQKHKLEEEDFRGERFKDYHMLIKGNNDLLSITKPDIIGGIYEKYLVEGGSQLIGTNTFSSTTIAQADYDMQSLIYELNYDSARLAREVCDKVTAQDPTKPRFVVGAIGPTNRTASISPDVNDPTARNCTFDELVEAYYEQCVGLVDGGADILIVETIFDTLNAKAAVFAVNEYLEHANV-DIPLIISGTLVDQSGRTLSGQTGEAFYASIRHAKPICVGLNCALGASAMAPFLKRLADCAECFVHVYSNAGLPNAMGGYDDTPQDMARENLMFAENGWVNMIGGCCGSTPPHIAAIQEAI--KDCKPRPLPTRKAPRMWLSGLEDFVVESAHNNVGLPFVNVGERCNIAGSRKFKRLVMEGKFAEAMDIAKDQVENGAHIVDVNVDDGMLDGMNAMQKFIKMAVTEPDVSKVPFMIDSSKFEIVEAGLKWCQGKPIVNSISLKVGEEKFKEHALCLRKHGAAVVVMAFDEQGQAASREDKVRICKRSYDILVSEPIYYPPEDIVFDPNILTIGTGIEEHNNYGVDFIAAVREIKQVCPWAKISGGVSNLSFGFRGVNIIRESIHAVFLHHACLDSGMDMGIVNAKEMLALSEVPKHLRGVCIDAVMNTRPDATERLLTATEAEKEAAEARKRGGGDVAVAKTTTWRDMPIEERITHSLVKGISEFVDKDTEELRAEIAARGGRPLEVIEGPLMAGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPYMEAEKRENLIAQGIDPDSQQQDDDSQYAGKFLIATVKGDVHDIGKNIVAVVLGCNNYKVYDIGVMCECDKILEKAKEYKVDAIGLSGLITPSLDEMVFVAKEMSKKGLQVPLLIGGATTSKMHTAVKIAPQYASDEHPVIHVLDASRAVTVVSSLLNADDKPDYVADIADEYAELRDEYYASLEDRKFLTLEQAQAKRCELNWSKVPPAP---KTALGAHTITYELSDVVPLIDWNPFFQTWELRGRYPNRGYPKIFNDETVGAEAKRLFDDAQKMMKQIVDEKRLELRGVYGLYAAQRSEGGEDVDVWDSDAARDAKEKPKATFCMFRQQAETESAAAYMSQADFIAPRGAA-KDYLGMFAVACFGAEEWANKYEAQNDDFSKIMMQALADRLVEACAEALHRDMRTKYWGYASDETLSAEDLIKVKYEGIRPAPGYPSQPDHTEKRTMWDLLDVESRSGIKLSESISMMPASSVSALVFAHPEATYFAVGKIEKDQVKSYAERKGYSLPEMERWLSPMLNYDRD 1367          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A1Z5K9H8_FISSO (Methionine synthase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K9H8_FISSO)

HSP 1 Score: 1688 bits (4371), Expect = 0.000e+0
Identity = 858/1292 (66.41%), Postives = 1029/1292 (79.64%), Query Frame = 0

Query:   15 ANYPYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRY----KLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLE-AGSDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVDEVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAV-KAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREK--ALRNKGKAT-AETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLGDRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPA-ATVGGEDVEVYANESREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            A++P++ RG+DS   ++ +LF  RI ++DGAMGTMIQ Y    KL E+EYRGER+ D+   VKGNNDMLSITQP II+ I+ +YLE  GSD+IGTNTFSSTTIAMADYEME   YELN+  AR+ARE C+E TA+DP  PRFV+G++GPTNRTGSISP VEDPS RNVTFDELV  Y +Q   L+DGG+D+++VETIFDTLNAKAA++A+ EY +  GL D+P+FVSGTLVDQSGRTLSGQT E FYAS+RH KP C+GLNCALG   M PF++RLA   ECF+HVYSNAGLPNAMGGYDD+P+ MA  N LF + G +NM+GGCCGSTP HI+A    +  +  EPR LP    P MWLSGLED+ V          FLNVGERCN++GSI+FKKL++ GDY  AMD+AKQQVEDGA VIDIN+DDG+LDG+ AMQKF KIAVTEP+V+KVPFM+D+SKF IV AGLKW QGK IVNSISLKVGE+ F  HA ++K HGAAVVVMAFDEEGQAA    KVRICKRSYDILV++V FPPEDIIFDPN+LTI TG+EEH NY VDFI A + IK  CPY KISGG+SNLSFGFRGV  IRES+H+VFL+HA+ ++GMD+GIVNA  + + +++EP LLK  E+ V NK   ATE +L+    E+E+  A +  G  T      WR Q    RL H+LI GI +FV +DVEE R     +  +PLDVIEGPLM GMN+VGDLFGAGKMFLPQVIKSARVMKKAVA+LLPFM+ EK   +IA G++P   ++ DDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNN+KVYD+GVM  C+ IL +A E +VD+IGLSGLITPSLDEMV VAKE++K G K PLLIGGATTSKMHTAVKI+P Y+S E PV+HVLDASRSVTVV +LLG+ K DYV ++ EEYEE+RE++YAG E+R++L+FE+A  +   IDF K PP   P + G T++ D   L DV+ YIDWNPFFQTWELRGRYPNRGYPKIF DE+VG+EAKKLF+DAQ+M+ +I+ + ++ LTG+VG++PA  +  GEDVEVY NE+  E     ARFCMLRQQAEKESDDPY SQADF+AP+  G+ DH+GMFAV+C+GCD +V ++E  NDDYSKIM QALADRFVEAFAE LH +IR  +WGYAP+EK+   DL K+KY GIRPAPGYPSQPDHTEK TMW+++ A EL GIELSESLSMMPASSVSALVFAHP +EYFAVG + KDQV SYAERK M ++ CE+WLSPILNY+R+
Sbjct:   80 ASFPHYVRGRDSLRKHITQLFTSRIAIYDGAMGTMIQNYAKKNKLEEDEYRGERFKDWKCNVKGNNDMLSITQPHIIQGIYRQYLEEGGSDLIGTNTFSSTTIAMADYEMEAYAYELNYWGARLAREVCDEVTARDPAKPRFVVGAIGPTNRTGSISPSVEDPSARNVTFDELVETYFEQVVGLMDGGADILMVETIFDTLNAKAALYAIGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAQHMTPFVERLAGCVECFMHVYSNAGLPNAMGGYDDSPETMAEQNRLFFENGWLNMVGGCCGSTPPHIRAIREVS--EGYEPRKLPDVGRPKMWLSGLEDMKVEDVHNHLGLPFLNVGERCNIAGSIKFKKLMMAGDYGAAMDIAKQQVEDGAHVIDINVDDGMLDGLTAMQKFVKIAVTEPEVAKVPFMLDASKFEIVLAGLKWCQGKPIVNSISLKVGEEMFIEHATLLKKHGAAVVVMAFDEEGQAATESEKVRICKRSYDILVNKVKFPPEDIIFDPNVLTIGTGMEEHANYGVDFINACKTIKEECPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAILESGMDVGIVNAKEMLMIEEVEPELLKASENLVFNKSEDATEVMLELTRKEKERFEAFKAGGGVTKTNEASWRDQDAVKRLEHALINGISEFVDQDVEEAR----QKCSKPLDVIEGPLMDGMNIVGDLFGAGKMFLPQVIKSARVMKKAVAYLLPFMDEEKRQNLIAQGLNPDDFDQNDDSNYAGKVLMATVKGDVHDIGKNIVAVVLGCNNFKVYDIGVMCSCELILEKAKEYDVDVIGLSGLITPSLDEMVVVAKELSKGGFKQPLLIGGATTSKMHTAVKIAPNYSSAEHPVIHVLDASRSVTVVSSLLGNAKEDYVADIHEEYEEMREDYYAGLEDRYFLTFEQAKQQKFMIDFEKSPPASQPKQIGTTLI-DKVKLSDVVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGKEAKKLFDDAQSMMKEIIADGSMWLTGVVGLYPANRSDDGEDVEVYENEADRETGTPKARFCMLRQQAEKESDDPYFSQADFVAPR--GMKDHLGMFAVSCLGCDALVDKYEKSNDDYSKIMAQALADRFVEAFAEYLHREIRVNMWGYAPDEKLNEADLLKIKYDGIRPAPGYPSQPDHTEKTTMWELIKAKELAGIELSESLSMMPASSVSALVFAHPQAEYFAVGQVGKDQVVSYAERKNMKLDVCERWLSPILNYERE 1361          

BLAST of mRNA_P-lacustris_contig911.15575.1 vs. uniprot
Match: A0A024USD9_9STRA (Methionine synthase n=18 Tax=Aphanomyces TaxID=100860 RepID=A0A024USD9_9STRA)

HSP 1 Score: 1688 bits (4371), Expect = 0.000e+0
Identity = 871/1282 (67.94%), Postives = 1032/1282 (80.50%), Query Frame = 0

Query:   18 PYFERGKDSTMAYLYRLFQERIVMFDGAMGTMIQRYKLGEEEYRGERYADYHVLVKGNNDMLSITQPQIIKKIHAEYLEAG-SDIIGTNTFSSTTIAMADYEMEKDVYELNFESARIAREACEEATAKDPTHPRFVMGSLGPTNRTGSISPDVEDPSFRNVTFDELVAAYKDQTKALIDGGSDMIIVETIFDTLNAKAAVFAVREYFDEQGLTDVPLFVSGTLVDQSGRTLSGQTTEGFYASMRHCKPFCLGLNCALGPTQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDDTPQDMARDNLLFTDEGLVNMLGGCCGSTPAHIKARNPATTCKHCEPRPLPLPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEDEFRRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDILVD-EVGFPPEDIIFDPNILTIATGIEEHNNYAVDFIQATERIKAICPYCKISGGVSNLSFGFRGVNAIRESMHSVFLYHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNKHPGATEALLDAAEIEREKALRNK----GKATAETTGWRTQGVEARLTHSLIKGIDQFVIEDVEECRLLLDAQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLLPFMEAEKLAKMI---ADGIDPSSVNEEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKVYDMGVMQPCDRILARALELNVDIIGLSGLITPSLDEMVFVAKEMAKAGIKLPLLIGGATTSKMHTAVKISPQYASPETPVVHVLDASRSVTVVGALLG-DRKADYVEELMEEYEELREEHYAGQEERHYLSFEKASCKGKKIDFHKRPPVPAPLKPGITVVTDTYALDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFLDESVGEEAKKLFNDAQAMLNKIVKEKTLSLTGIVGIFPAATVGGEDVEVYANESREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGLADHMGMFAVACMGCDQMVKEFEDLNDDYSKIMVQALADRFVEAFAEKLHEDIRKTIWGYAPNEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDVLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDVETCEKWLSPILNYDRD 1289
            PY  RG+     YL  L   +I++FDG MGTMIQ++K  EEEYRGER+ D+  LVKGNND+LSITQ   IK IH EY+  G + +IGTNTFS TTIA ADY ME  VYE+NFESA +AREAC+E TA DP  PRFV GS+GPTNRT SISP+VEDP FRN+TFDELV AY +Q +AL+DGGSD+++VETIFDTLNAKAAVFAV +Y +++G   +PLF+SGT+VD SGRTLSGQTTE FY S+RH KPFC+GLNCALG  QM+PFL+RLA VAECFV VY+NAGLPNAMGGYDD P  MA+    F +EGL+NM+GGCCG+TP HIKA   A   K  +PRP  +P+EPFMWLSGLEDLIVTKERF FLNVGERCN+SGSIRFKKLILKGDY TAM++A+ QVE+GAMV+D+N+DDG+LDG++AM++F KIAVTEPDVSKVPFMIDSSKF +VEAGLK VQGK IVNSISLKVGEDEF RHA+IVK+HGAAVVVMAFDE GQAA  + KVRICKRSYDILV   VGFPPEDI+FDPNILTIATG+EEHNNY VDFI A   IK   PYCKISGGVSNLSFGFRGVN IRE++HSVFLYHAV+AGMDMGIVNAGML+VYDDI   LL+IVED V N++P A+E LL+ + +EREKA   K    G A A+   WRT+ V  RLTH+L+KGI  ++  DVEE RLL +    RPL VIEGPLM GMNVVGDLFG+GKMFLPQVIKSARVMKKAVA+LLPFMEAEKLA+     ADGI  + ++E  +S YAGKVL+ATVKGDVHDIGKNIV VVLGCNNYK+ D GVM PC+ IL  A E NVDIIGLSGLITPSLDEMVFVAKEMAKAG+  PL+IGGATTSKMH AVKI+PQY++ + PV+HVLDASRSV VVG LL  D KAD+ EE++EEYEELRE++YA  ++   ++++K   K   ID+   PP     + G  VV D  AL D++ +IDWNPFFQTWELRGRYPNRGYPKIF DE+VG EAKKLF+DAQ ML++I+  K++ + G+ GI+ AA  G +DV +Y  E R+E +A F MLRQQAEKE+D+PY+S  DF+AP E+GL DH+GMFAV C G +++  E+E  +DDYSKIM QA+ DRFVEAFAE +H ++R   WGYA +E +  +DL K+KY GIRPAPGYPSQPDHTEK+TMW++L A E  G++LSE+  MMPASSVSAL FAHP+S+YFAVG + KDQ+++YA+RK   VE  EKWLSPIL+YDRD
Sbjct:   13 PYV-RGETDLFEYLTALMSTKILIFDGGMGTMIQKHKFTEEEYRGERFKDWEHLVKGNNDLLSITQRDTIKAIHKEYMTVGGAQLIGTNTFSGTTIAQADYHMEDLVYEINFESAALAREACDELTALDPLTPRFVAGSIGPTNRTLSISPNVEDPGFRNITFDELVQAYYEQVEALVDGGSDILLVETIFDTLNAKAAVFAVNKYQEDKGKR-IPLFISGTIVDMSGRTLSGQTTEAFYVSLRHSKPFCIGLNCALGANQMKPFLRRLANVAECFVSVYANAGLPNAMGGYDDDPLLMAKYCGEFCEEGLLNMIGGCCGTTPLHIKAI--ADEAKKSKPRPQYIPKEPFMWLSGLEDLIVTKERFAFLNVGERCNISGSIRFKKLILKGDYGTAMEIARAQVEEGAMVVDVNVDDGMLDGVSAMERFLKIAVTEPDVSKVPFMIDSSKFHVVEAGLKCVQGKCIVNSISLKVGEDEFIRHAKIVKSHGAAVVVMAFDEFGQAATEEEKVRICKRSYDILVGPRVGFPPEDIVFDPNILTIATGMEEHNNYGVDFINACRVIKEQNPYCKISGGVSNLSFGFRGVNVIREAIHSVFLYHAVQAGMDMGIVNAGMLQVYDDIPKDLLQIVEDVVLNRYPEASELLLERSLLEREKAEAAKKGGTGAAVAQQE-WRTKPVSERLTHALVKGISDYIDADVEEMRLLCE----RPLHVIEGPLMDGMNVVGDLFGSGKMFLPQVIKSARVMKKAVAYLLPFMEAEKLAQQAKDRADGIVSADMDE--NSMYAGKVLLATVKGDVHDIGKNIVGVVLGCNNYKIIDAGVMVPCEEILRLAKEHNVDIIGLSGLITPSLDEMVFVAKEMAKAGMTQPLMIGGATTSKMHAAVKIAPQYSTIDHPVIHVLDASRSVVVVGNLLKEDEKADFAEEILEEYEELREDYYASIDDIKMIAYDKICAKSFAIDWTASPPYGKTNQLGNRVV-DGVALADIVPFIDWNPFFQTWELRGRYPNRGYPKIFDDENVGAEAKKLFDDAQKMLDEIIANKSMQVRGVCGIYRAARKG-QDVVLYHPERRDEEIATFAMLRQQAEKETDEPYMSLCDFVAPVETGLEDHLGMFAVGCFGVEELAAEYEAKHDDYSKIMAQAIGDRFVEAFAEYIHREMRTKFWGYAADESLVQEDLLKVKYDGIRPAPGYPSQPDHTEKKTMWNLLQA-EALGLKLSENFVMMPASSVSALCFAHPESQYFAVGKVGKDQITAYAQRKKQSVEFTEKWLSPILSYDRD 1280