mRNA_P-lacustris_contig976.16074.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: D8LK54_ECTSI (Calpain catalytic domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LK54_ECTSI)

HSP 1 Score: 1058 bits (2735), Expect = 0.000e+0
Identity = 520/625 (83.20%), Postives = 545/625 (87.20%), Query Frame = 1

Query:    1 MPRLSAYEEAFDEDDEEEIGDTEEGDIIARLQASQRENLYEDGSFPPNRSSL--NTKSVPEYDADLSEVKWLYPHEICEAPVYFSDDANAGSGYLSRGRLDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACVQADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHTTHKRIWEQKQNDVIASSHDNTFASTRGEREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDTAGGPLRLVDENKGVKNNPKWCQNPQYLLNIPARAQYESVDVKLVLRR 1863
            MPRLSAYEEAFDEDDEEE+GDTEEGDIIARLQASQR  LYED SFP  RSSL  N KSVPEYDAD+SEVKWLYP EIC+ P YFSDD   GSG L +GRLDDTWLLGALGVLASHPG+LIENLFASEPDDFVR+GVYT  FYKNGEWQEVVTDTRIPCA  SK+KGDD LLGQKVPG PTP+YGHGLNLNEQWV MLEKAYAKLHG+YESINGGSVGEALVDLTGGCCEKMS TSER+KPM+EDGSLWETL  HVSP+YVVACVQ                                 CSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVY+EVSSD NTPWRRDSNDGTFWMVFSDFV LFTK+Y CRVFPD+ YRQYCIHGEWSGKTAGGSAKPM+RGSVSRLQKD QDKMKDSLRRESVVDIQDDGDPYWFNNPQYRL+ADE VEVYISLMQQDRRSAN+LRENYR+GFEVVQTKRHTTHKRIWEQKQ+DVIA SH+NTF STR EREVTR SVKL+PRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEP+ ETFT++LHGSWDRTSDRDTAGGPLRLVDENKGVKNNPKWCQNPQYLLNIPAR         LVL R
Sbjct:    1 MPRLSAYEEAFDEDDEEEVGDTEEGDIIARLQASQRGALYEDVSFPAKRSSLYRNIKSVPEYDADVSEVKWLYPQEICQEPAYFSDDP--GSGCLKKGRLDDTWLLGALGVLASHPGILIENLFASEPDDFVRYGVYTCRFYKNGEWQEVVTDTRIPCAHNSKNKGDDILLGQKVPGNPTPMYGHGLNLNEQWVSMLEKAYAKLHGSYESINGGSVGEALVDLTGGCCEKMSLTSERIKPMMEDGSLWETLRSHVSPEYVVACVQE--------------------------------CSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYSEVSSDTNTPWRRDSNDGTFWMVFSDFVGLFTKVYQCRVFPDEDYRQYCIHGEWSGKTAGGSAKPMRRGSVSRLQKDGQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLTADETVEVYISLMQQDRRSANHLRENYRVGFEVVQTKRHTTHKRIWEQKQDDVIADSHENTFGSTRSEREVTRASVKLDPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPVTETFTSYLHGSWDRTSDRDTAGGPLRLVDENKGVKNNPKWCQNPQYLLNIPARCDELHKRGALVLPR 591          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A7S2D2G9_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2D2G9_9STRA)

HSP 1 Score: 723 bits (1865), Expect = 1.890e-244
Identity = 406/946 (42.92%), Postives = 550/946 (58.14%), Query Frame = 1

Query:   64 TEEGDIIARLQASQRENLYEDGSFPPNRSSL--NTKSVPEYDADLS-EVKWLYPHEICEAPVYFSDDANAGSGYLSRGRLDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACV-QADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKD---------------SQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHTTHKRIWEQKQNDVIASSHDNTFASTRGEREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDTAGGPLRLVDEN-KGVKNNPKWCQNPQYLLNIPARAQYES-VDV-------KLVLRRQGDESHGRGKEASGSKPRRTSLTHNALTGLVICKARHPEDGAPLRRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQVAAASKDDQHSSSV-LPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQTAGGSHIHPHWKKNPCFHLKLRTTGPAKVRISVSRP--EKDWKNKCIRDSVGCMMGFYLMSGPKPNRDHT-IFHEGRPYEGSPMVPTDEVSTPPGFMLNTTSEDDVFTIMPATFEPGKTGPFFISIATTDVDFSLR 2799
            T E DIIA L  S   N   D +FPP+ +SL  N   V +YD+     ++WL   E  + P YF++ A      LSRGRL+D WLLGA+  +A+HP  LIENLF S PDDF R+GVYT   YKNG W+E+V DTR+P A  S        L Q       PIYG   N+NEQWV +LEKA+AK HG YE +N G V E LV+LTGG CE ++  S+ V+ M++ G LW+ L  H+   ++V CV +A      S+D  TGLL NHAY +  +K        GF  VR+R+PW  G W G+WSD S  WED+PEV   +  D +  W R S DGTFWM F+DF  +FTK+++CRVFPD +YRQYCI   WSGK+AG                                + +  K   +      ++ DG+  WFNNPQY+++A E   ++ISLMQ+DRR+   L++N++IGFEVVQ K    HKR W    +DV+  +  N       +REV   ++ L P   YNIVPH  +Q +E  + LRIFS  D+ ++ + ++ +  L G+W R ++RD+AGGPL +     K   +NPKWC+NPQ++L +P      S VDV                                     +   GLV CK   PED    RR V   RR+NALGE + TKESSLK P         +   + +Q +S V +PDRK+V++  EW Q +DY+ ++ + LML N+  ++ P+GL L+PTLSE G KG + LEVHSD+PV VE LP+T SRT+A EW    +GGSH+H  WKKNP +HL+L     +KV+IS+SRP     WK +  +DSVG M+GFYL+ GP P RD   ++H+G+P+  +P VP + VSTP  F+L    ED+V++I+PATFE GK GPFF+S+   D DFSL+
Sbjct:   16 TAEDDIIAMLHESGATNFC-DPAFPPDTTSLYRNKNFVADYDSRFGGNIRWLRCTEFAQQPDYFAE-ATESKHVLSRGRLNDVWLLGAMAAVAAHPDALIENLFGSAPDDFKRYGVYTCRLYKNGVWEEIVADTRVPTAEMSAPVAGLKPLAQ---ASFCPIYGRCDNVNEQWVSLLEKAFAKYHGNYECLNHGDVAEGLVELTGGSCETIALNSKPVQEMVKSGKLWDMLTLHLEDQHMVTCVVEAGDEDDYSVDESTGLLNNHAYSVLMVKHVL-----GFEFVRVRSPWGGGVWTGEWSDDSPKWEDFPEVLENLKDDASVNWERRSYDGTFWMKFTDFCNIFTKIHVCRVFPDDEYRQYCIQSSWSGKSAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASEYPKLVAKHARADAVKADGNDTWFNNPQYQVTAAEPCNIHISLMQEDRRTDTRLKDNFQIGFEVVQMK-DPEHKRAWGPN-DDVVFDTASNPLNGDLLQREVCGSNIHLEPGTTYNIVPHAAQQGKESPYILRIFSPVDLEIKEVPQSHSVTLPGAWQRVTERDSAGGPLLVPGSGGKSCASNPKWCENPQFVLRMPKHVPTSSSVDVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLKVGLVACKIPAPEDERS-RRRVPGERRINALGEEMPTKESSLKNPR--------SHFLQQNQDASEVEMPDRKMVIDAKEWYQTTDYKRSDVAMLMLPNVSVNWAPHGLLLIPTLSEIGAKGQFVLEVHSDYPVVVESLPDTKSRTLAGEWTESNSGGSHMHQSWKKNPLYHLRLLGNKRSKVKISLSRPAGNASWKAQTGKDSVGSMIGFYLVQGPAPTRDAAEVYHDGKPWSETPFVPMNSVSTPSNFVLEPL-EDEVYSIIPATFEAGKKGPFFLSVVA-DCDFSLQ 938          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A836CKR5_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKR5_9STRA)

HSP 1 Score: 631 bits (1627), Expect = 2.420e-212
Identity = 338/718 (47.08%), Postives = 444/718 (61.84%), Query Frame = 1

Query:    1 MPRLSAYEEAFDEDDEEEIGDTEEGDIIARLQASQRENLYEDGSFPPNRSSLNTKS--VPEYDADLSEVKWLYPHEICEAPVYFSDDANAGSGYLSRGRLDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACVQADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPW-SHGEWKGDWSDASTLWEDYPEVYAEVSSDPNT--------------------PWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQ-YRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRH---------TTHKRIWEQKQNDVIASSHDNTFASTRGEREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDTAGGPLRLVDE---------------NKGVKNNPKWCQNPQYLLNIPARAQYESVDVKLVLRRQGDESHGRGKEASGSKPRRT-----------SLTHNALTGLVICKAR 1971
            M +L A +++ DE+D+EE+G++EE D+I  L+ S     YED SFP ++S+L   S  VPEYD  + E+KW+ PHE+   P YF+D  +AG+G + +GRLDD WLLGA+  +A+HP  LIENLF SEP DF  +G+YT  FY NGEWQE+VTDTR+PC       G +   G     G  P+YG G+++NE WVP+LEKAYAKLHGTYE+++GGSVGEALVDLTGG   ++     RV+ M+ DG LW+ L R++   Y++A                G+ +N A  L  ++       SGFRLVR+R+PW +   W GDWSDAS  WEDYPEV A +S+                        PWRRD  DGTFWM + DFV  FT +Y+CRVFPD   +RQYC+HGEW+G   G +A P           D+   + +SLRR ++VD+Q DGDP+WFNNPQYRL+ D+  EV ISLMQQDRRSA+ LREN+ +GFEV++ +R            H R+WE++  DV+  S   +FA  R EREV +  V L+PR AY +VP   E  REGKFTLR+F++ DV +EP+ ET T +L G+WDRTS+RDTAGGPLRL +E                +GV+NNPKWCQNPQ+ L IP  A Y+SVDVKLVLRR  D   G      G KP +            +  H+ + G  +C+AR
Sbjct:    1 MGKLEALKDSMDEEDDEEVGESEEADVIENLRESGSGKRYEDTSFPASKSALYRSSTIVPEYDMGVGEIKWMRPHELARDPTYFND-GSAGAGNVVKGRLDDAWLLGAMAAVAAHPSNLIENLFTSEPGDFNNYGIYTCRFYINGEWQEIVTDTRLPCTPLPP--GAEPPAGAAC--GHVPLYGRGVDINEFWVPLLEKAYAKLHGTYEALSGGSVGEALVDLTGGSTARICLEEARVEAMVGDGRLWDRLRRYLRWQYILAA---------------GVPLNAACSLVGLREV-----SGFRLVRVRSPWGAAAAWTGDWSDASPRWEDYPEVLAAISAGXXXXXXXXXXXXXXXXVDAVGGGPWRRDGGDGTFWMAWEDFVARFTHVYVCRVFPDGAGFRQYCVHGEWAGDAMGLAAAPAXXXXXXXXXXDA---VAESLRRTTMVDVQPDGDPFWFNNPQYRLTVDKPTEVLISLMQQDRRSASYLRENHGVGFEVLRARRRGGSXXXXXAAPHARVWERRAEDVVTDSARASFAPARPEREVAKAGVTLDPRLAYVVVPTCAELGREGKFTLRVFAQSDVVMEPVPETHTLYLRGTWDRTSERDTAGGPLRLPEEAPSGSXXXXXXXXKEGRGVRNNPKWCQNPQFWLRIPDGAAYDSVDVKLVLRRVADGPGGXXXXXRGGKPPKVRARAGVARQHCAAEHHLIAGF-LCRAR 689          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A7S1U0F0_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U0F0_9STRA)

HSP 1 Score: 610 bits (1573), Expect = 3.680e-200
Identity = 380/1006 (37.77%), Postives = 534/1006 (53.08%), Query Frame = 1

Query:   70 EGDIIARLQASQRENLYEDGS--FPPNRSSL--NTKSVPEYDADLSEVKWLYPHEICEAPVYFSDDANAGSGYLSRGRLDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACVQADPNRGTS-----MDSD---------TGLLINHAYGLADMKACALYLCSGFRLVRMRNPWS-HGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDS-NDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAK-PMKR----------------------GSVSRLQKDS---------QDKMKDSLRR--ESVVDIQDDGDPYWFNNPQYRL-SADEVVEVYISLMQ-QDRRSANNLRENYRIGFEV--VQTKRHTTHKRIWEQKQNDVIASSHDNTFASTRGEREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDTAGGPLRLVDE-----NKG-------------------------VKNNPKWCQNPQYLLNIPARAQ---YESVDVKLVLRRQGDESHGRGKEASGSKPRRTSLTHNALTGLVICKARHPEDGAPLRRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQVAAASKDDQHSSSVLPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQTAGGSHIHPH-WKKNPCFHLKLRTTGPAKVRISVSRPEKDWKNKCIRDSVGCMMGFYLMSGPKPNRDHTIFHEGRPYEGSPMVPTDEVSTPPGFMLNTTSEDDVFTIMPATFEPGKTGPFFISIATTDVDFSLRRD 2805
            E +IIA+L+      L  DG+  F  +  +L  N   VP YD    + +WL P +    P YF    +     L  GRLDD W LGAL  +ASHP  L+E LF S  DDF R+GVYT  FY NGEW+EV+ DT++P      +   D    ++ PG   PIYGH  +  E WV  +EKAYAKL+G+YE++N GS  EALVDLTGG C ++   S+  K +   G LW+ L  H++ D  + C Q +P   +       D+D         TGLL N  Y +  ++           LV +RNPW   G+W+GDW + S +WEDYP+V   ++S   TPW  D  N+G FWM F DF   FT L++CR+F D  Y QYC+ G+W+GKTA G  + P++                       G +S    D          ++   +SL R  E    + +DG+P+WFNNPQ+R+ +      +Y+SL Q Q RR+    R    IGF +  + ++      RIWE K  DVIA S  +  AS     EV    V L P   Y IVPH  ++NREG+F LR+F R  + +  + ET + +L G+W+RT ++D+ GGP+ +V+E     NK                          +K NPKWCQNPQY +     +     +S  +K V+RR       R +E +  KPR  +   +   G+V  K R        RR     RRVNALG+PL TKES+LKR T  R       A+ D+    +  P+    +   +W +M+ Y + E + L L N+      +GL + P LS +G KG Y LEVHS  P+ +E L + + +T+A EW   +AGGSHIH   WKKNP F L+L +TGPA+V++ +SRPE  W  K +R++VG MMGFY+  G +PNR       G+P+  S  VP+ +V TP  F+L    +DD +TIMPAT++PG  GPF +++ T++  FSLRR+
Sbjct:    6 EEEIIAQLE------LQADGTSIFEDDWKALYRNPSQVPAYDEHGGDFRWLRPAQYLRRPEYFHWVGDRVQ--LQAGRLDDAWFLGALAAVASHPAGLVEALFGSNADDFKRYGVYTCRFYLNGEWREVICDTKLPVEDKPVNPFADMEAAEEGPG-MAPIYGHSAHPEELWVSFIEKAYAKLNGSYENLNEGSPAEALVDLTGGSCVELDMASDTAKQLAGSGELWDLLLAHIN-DGGILCGQVEPPNASEGKEELADADPALVEPDPVTGLLPNRLYTVIALREVP----GAPPLVLVRNPWGIEGDWEGDWGEGSAMWEDYPDVEEALASGNTTPWTTDGENNGLFWMPFVDFCENFTTLFVCRIFDDDAYYQYCVEGDWAGKTAAGPPRRPLRAAQGVLDFNAVYPQGKKNEKKGAGGISPKASDGAAAPSANGQKEAYAESLARHFEGSKMLCEDGEPFWFNNPQFRVVTGSRPCRIYVSLQQRQGRRAGAAKRVGPSIGFHLLKIDSEAEGVLGRIWELK--DVIADSAQDLMASKEALGEVVCQDVTLEPDTKYVIVPHAGQKNREGRFCLRVFCRSALDIASVTETSSVYLPGAWERTPEQDSCGGPVLVVEEESKPANKAGMPLTPPPTEPEEEGAQPETVTLLKMKQNPKWCQNPQYWVRFAETSDDPLAQSATIKFVVRRTD-----RRQEGTRRKPR--AGADDVQVGVVAVKVRGRATSVTSRRRQQNKRRVNALGKPLPTKESTLKRRTVNR------NATGDEMQVEA--PETLKCINAADWHRMNLYENVETACLTLTNVTSASFTDGLIVSPCLSSRGTKGTYVLEVHSSLPLRIEGLTDGADKTLAGEWAEGSAGGSHIHTDSWKKNPKFQLRLNSTGPAQVKVRLSRPEDYWAPKMLRNAVGAMMGFYIYQGQRPNRGAVTLVNGKPWSDSAFVPSLDVCTPQNFILEPLPDDDFYTIMPATYDPGNHGPFHLTV-TSEAPFSLRRE 979          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A7S1C892_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1C892_9STRA)

HSP 1 Score: 558 bits (1438), Expect = 2.060e-181
Identity = 341/913 (37.35%), Postives = 503/913 (55.09%), Query Frame = 1

Query:   67 EEGDIIARLQASQRENLYEDGSFPPNRSSL--NTKSVPEY---DADLSEVK--WLYPHEICEAPVYFSDDANAGSGYLSRGRLDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACVQADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHTTHKRIWEQKQNDVIASSHDNTFASTRGEREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDTAGGPLRLVDENKGVKNNPKWCQNPQYLLNIPARAQYES-VDVKLVLRRQGDESHGRGKEASGSKPRRTSLTHNALTGLVICKARHPEDGAPLRRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQV--------AAASKDDQHSSSVLPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQTAGGSHIHPHWKKNPCFHLKLRTTGPAK-VRISVSRPEKDWKNKCIRDSVGCMMGFYLMSGPKPNRDHTIFHEGRP--YEGSPMVPTDEVSTPPGFMLNTTSEDDVFTIMPATFEPG 2742
            EE +I+A L+  +   LYED SF PN +SL  N   +PEY   +  LS+    WL PHE  +AP  F     AG   + +G+L D WLLG+L  +A+HP  LI+NL  S+ D +   GV +  F+KNGEW  V TDT IP        GD A      P    PIYG   +++E WVP +EK YAKLHGTYE +NGGSV EA+VD TGG  EK   T E VK  I  G LW  +    S ++++ C  +    G   D+ +G+LINHAY +  +K          + V++RNPW HGEW+GDWSD    W+ +P+V + +  DP   + R   DGTFWM++ DF + F K+Y+CRVF ++ Y QY +HG W G +A GS K M+    +  +KD+ +  K S R+        DGD  WF+NPQYR++  +  +  ISL Q DR       EN  I F V++  +++  +R+WE+  +D++A S+ + FA+   +REV    V+ +P+Y+Y IVPH M++  E  F LRIFS  ++ V+ + + +   L  +W R    D AGGP R+       + NP +C NPQ+ + +P  +  +    +KLVL R  D + GR  ++ G+K R          GL + + + P    P RR     R  NA+GEPL +KESSL+RP       +         A A+  + H     P RK+ V   E+   S Y   + +T +LK +  D++ +GL + P +   G +G ++L++ S+  + ++EL +  SRT+   W+ + A G H+HP WK+NP F + L+    A   +I++SRPE  WK     DSVGCM+GFYL++  +  RD + F++GR   ++ +  VP  + S       +   E  VF ++PATFEPG
Sbjct:   37 EEDEILATLRDGE---LYEDRSFAPNDASLYHNLSVIPEYANVEPRLSKGPHPWLRPHEFAKAPELFKAGTEAGD--VVQGQLGDCWLLGSLASVAAHPDDLIDNLIVSDDDTWKTSGVVSCQFFKNGEWVTVTTDTMIPY-------GDPA-----DPAFNMPIYGRCRDISELWVPFIEKCYAKLHGTYEGLNGGSVAEAMVDFTGGVAEKFLLTDEHVKARIASGELWRDMMELKSGNHLLGCSMSRFGGGFETDAGSGILINHAYSILHLKEVG-----SLKFVKIRNPWGHGEWRGDWSDNWPKWDQHPDVESALMDDPACKFNRSDADGTFWMIWEDFTKQFNKIYVCRVFGEE-YNQYVVHGRWEGLSAAGSHKVMEDRDGAGEEKDADETEKQSKRQ--------DGDAQWFHNPQYRVTCTKPAKACISLTQSDRMLTGRA-ENVAIRFVVLRCPKNSK-QRVWEEDPSDIVADSNASNFANMFAQREVVHPEVEFDPKYSYVIVPHTMQRGMELGFNLRIFSEAELAVDAVPDPYGIELSSAWIR----DGAGGPRRVDG-----RANPAFCLNPQFWITLPPGSDTKKKTTLKLVLTRT-DRNAGRDDKSDGTKNR---------VGLTVTRVQPPPPDNPRRRRPGQVR-TNAMGEPLPSKESSLRRPLGDTAASEAKGGAGGMGATAAAAEPHDP---PKRKLDVTNEEYTLTSYYTDAKVATTLLK-VPADWLRDGLLVHPAMKLPGREGEFTLQLFSETQLMMQELSQGHSRTVTGSWSDKNAMGCHLHPEWKRNPAFQIVLQDRPVAMDCKITLSRPEDRWKAMARADSVGCMIGFYLVTCDR-ERD-SKFNDGRKDFFQETSFVPMHQQSLTTSLRFDLLPEGKVFLVIPATFEPG 890          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A1V9ZBZ2_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZBZ2_9STRA)

HSP 1 Score: 536 bits (1380), Expect = 9.570e-173
Identity = 337/949 (35.51%), Postives = 499/949 (52.58%), Query Frame = 1

Query:   49 EEIGDTEEGDIIARL-QASQRENLYEDGSFPPNRSSLNTKS--VPEYDADLSE------VKWLYPHEICEAPVYFSDDANAGSGYLSRGR-LDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTP-IYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERV-KPMIEDGSLWETLHRHVSP-DYVVACVQADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHTTH-KRIWEQKQNDVIASSHD---NTFASTRGEREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRD------TAGGPLRLVDENKGVKNNPKWCQNPQYLLNI-PARAQYESVDVKLVLRRQGDESHGRGKEASGSKPRRTSLTHNA-LTGLVICKARHPEDGAPLRRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQVAAASKDDQHSSSVLPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQ--TAGGSHIHPHWKKNPCFHLKLRTTGPAKVRISVSRPEKDWKNKCIRDSVGCMMGFYLMSGPKPNRDHT-IFHEGRPYEGSPMVPTDEVSTPPGFMLNTTSEDDVFTIMPATFEPGKTGPFFISIATTDVDFSLRRD 2805
            E  G T E ++IA L +      +YED  F     SL  KS  +PEY     +      V W  P+++C  P YF   + +G G L  G  L+D WLLG L  LA HP  LIENLF S+  DF  +G+YT  FYK+ +W EVVTDTR+P +       D  +    V   P   +YG  LN NE ++P+LEKAYAK HG YE+++ GS+ EA +D TGG  + +   S+   K +IE GSLW  L +H++    V++C     +   +  +  G+L N  Y +  +K          R +++RN W  G WKGDW +  + WED  +V A + +DP   + R  NDGTFWMV+ DF+  F +L++ R+FP   Y QYC+ GEW G+ A G    +          +S+DK ++S  R   V  Q D +P WF NPQYR+  +E   V ISL+Q+D R      +N+ I F V+Q K+   +   +WE  +  ++A +H     T +S   ERE+++ ++ L P  AY IVP+   Q  E +F LR FS K   ++ +    T  +HG W R+SD +      TAGGPL L     G++N   WCQNPQY L   P      S+ VK+V+++   ++         SK RR +    A L G+ + +   PE    + +     +  + LGE                            +     +PDRK++V+  E C +S Y S   +TL L+ +  +++  GL +VPTL E+  +G + +E+HSD PV V+E+   +++T+  EW+    T GG+H++P WKKNP F+L L+   PA V I + R E +W+ KC +DSVG MMGFYL  G K  RD + +  +GR +  +  VP   + +P   +    +E   + IMP T+EP +TG F +S++  D DF+L  D
Sbjct:    4 EGAGITLERNVIAMLSECGDIPPMYEDPDFAAKPVSLYVKSDVIPEYATSAKKGDDGTIVHWYRPNQVCTDPDYFK--STSGCGTLREGTGLNDCWLLGVLAALALHPDNLIENLFVSQMHDFKTYGIYTCQFYKDCQWLEVVTDTRLPYSQELLEVNDCRVANSNVMDQPGHWLYGSSLNKNEVFIPLLEKAYAKFHGNYETLHNGSIVEAFIDCTGGSVKVVDLASDSTRKELIETGSLWHKLVKHLNQFKSVLSCQLKMASMAYNDVTSAGILKNRLYAILHLKELG-----SLRFLKLRNVWQRGNWKGDWCNDDSKWEDNLQVEAALRADPACDFNRTKNDGTFWMVWEDFIEAFNELFITRIFPTSVY-QYCVRGEWIGQGAAGPPTKI----------NSEDKAQESTSRRWTV--QGDSEPAWFRNPQYRIVVEEKTTVLISLLQRDFRVFGG--DNFAINFGVIQVKKKALNASMVWEYDKEHIVAEAHSYEPGTESSQHPEREISKGNIVLEPDRAYIIVPYTDHQGVEMEFFLRFFSPKSCHIDALRPLNTLVVHGKW-RSSDENGNSNTSTAGGPLCLNLSTTGLENLA-WCQNPQYTLRANPKNDNQRSITVKIVVKQTSYKAT--------SKARRDASKERAHLLGITLVRPDVPEVDTGVHKKTKHDK-TDFLGE-------------------------NKGKCKIGAIPDRKLLVKADEMCTLSSYSSPSIATLFLQKINPEWLAQGLIIVPTLGERMTEGTFEMEIHSDDPVTVDEIRSDTTKTVFGEWSEDKSTTGGAHLNPDWKKNPKFYLTLQCVRPAAVVIHLYRAEHEWRTKCKKDSVGTMMGFYLFQGNKIQRDSSSVTVDGRSWTETDFVPMHHIQSPQLSLPALFNES--YCIMPTTWEPNRTGRFLLSVSA-DCDFTLTSD 891          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A1V9YCY5_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YCY5_9STRA)

HSP 1 Score: 521 bits (1343), Expect = 3.780e-167
Identity = 333/949 (35.09%), Postives = 495/949 (52.16%), Query Frame = 1

Query:   49 EEIGDTEEGDIIARL-QASQRENLYEDGSFPPNRSSL--NTKSVPEYDADLSE------VKWLYPHEICEAPVYFSDDANAGSGYLSRGR-LDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERV-KPMIEDGSLWETLHRHVSP-DYVVACVQADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHTTHKRI-WEQKQNDVIASSHDNTFASTRG-------EREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDT-----AGGPLRLVDENKGVKNNPKWCQNPQYLLNIPARAQYES-VDVKLVLRRQGDESHGRGKEASGSKPRRTSLTHNA--LTGLVICKARHPEDGAPLRRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQVAAASKDDQHSSSVLPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQ--TAGGSHIHPHWKKNPCFHLKLRTTGPAKVRISVSRPEKDWKNKCIRDSVGCMMGFYLMSGPKPNRDH-TIFHEGRPYEGSPMVPTDEVSTPPGFMLNTTSEDDVFTIMPATFEPGKTGPFFISIATTDVDFSL 2796
            E  G T E ++IA L +      +YED  F     SL  +   VP+Y     +      V W  P+++C  P YF   + +G G L  G  L+D WL+G    LA HP  LIENLF S   DF  +G+YT  FYK+ +W EVV DTR+P +    S+ +D  + + V      +YG  LN NE ++P+L KAYAK HG YE ++ GS+ EA VD TGG  +K+  +S+   K +++ G LW  L +H S    V+ C     +   +  +  G++ N  Y +  +K          R V+++N W  G WKGDWS+    WED  +V A + +DP   + R  NDGTFWM++ DFV  F +LY+ R+FP   + QYC+ GEW G  A G   P K  +  R +  S      + RR +V   Q + DP WF NPQYRL  ++   V +SL+Q+D R      +N+ I F +++ K+   H  + WE  +  V+A +H    A+  G       ERE+++ SV L P  AY +VP+      + +F LR+FS K   ++ +       + G W  T D  T     AGGPL  V        N  WCQNPQYLL +P RA  +  V +K+V ++   +          +K RRT    +   L G+ + +   P + A   +    P + + LGEP+     + K P                      +P+RK++V+  E C +SDY S   +T+ L+ +  D++ +GL +VP+L E  V+G + LEVHSD  V +EE+P   ++T++ EW+    TAGG+H++P W+KNP F+L L+   PA V I + R E +W+ KC +DSVG MMGFYL  G K  RD  T+  +GR +  +  VP   V +P   +    +E   + IMP T+EP ++G F +S++  D DF+L
Sbjct:    4 EGAGITLERNVIAMLSECGDIPPMYEDPDFAAKVVSLYVDVDKVPDYATSSKKGDEGGIVHWYRPNQVCIEPDYFK--STSGCGTLREGTGLNDAWLIGVFAALALHPDNLIENLFVSGMHDFKTYGIYTCQFYKDCQWLEVVADTRLPYSQ-ELSEVNDPRVAKPVAQPGHWLYGSSLNKNEVFIPLLAKAYAKFHGNYEVLHNGSILEAFVDCTGGSVKKVDLSSDTTRKSLLDTGLLWPKLVKHQSHYKSVLTCQLKLASMAYNDVTTAGIVKNRLYVVLHLKELG-----PLRFVKLKNVWQRGAWKGDWSNDDAKWEDNLQVEAALRADPACDFNRTKNDGTFWMIWEDFVETFNELYIARIFPSTMH-QYCVRGEWIGPAAAGP--PTKPAAEPRAEDPS------AARRWTV---QGESDPSWFRNPQYRLVVEDKTTVLLSLLQRDFRLYGG--DNFAINFVLLEVKKKALHASVVWEYDKTRVVAEAHSYVAAAGEGAKCTAHPEREISKGSVVLEPGRAYVLVPYTDHAGVDMEFFLRVFSAKPCTIDALRPLHALAVQGKWRATDDGGTGNSGSAGGPL-CVGLGASGPENLSWCQNPQYLLRVPPRADNKRPVTIKIVAKQTSYKV--------ATKARRTDAQKDKAHLLGIAVVRPDAPAEDAGAGKKRVKPEKTDFLGEPVGPARPAPKAPP---------------------MPERKLLVKADEMCTLSDYASPTIATVFLRKVNPDWLFHGLLVVPSLGEPLVEGTFELEVHSDDVVTLEEVPSIMTKTISGEWSEHKNTAGGAHLNPDWRKNPKFYLTLQCVRPAAVVIHLHRSEHEWRTKCKKDSVGTMMGFYLFQGTKIQRDSSTVIVDGRQWTETDFVPMHHVQSPHLSLPALFNES--YCIMPTTWEPNRSGRFLLSVSA-DCDFTL 897          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A024UDH4_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UDH4_9STRA)

HSP 1 Score: 519 bits (1337), Expect = 4.850e-166
Identity = 341/953 (35.78%), Postives = 496/953 (52.05%), Query Frame = 1

Query:   49 EEIGDTEEGDIIARL-QASQRENLYEDGSFPPNRSSL--NTKSVPEY-------------DADLSEVKWLYPHEICEAPVYFSDDANAGSGYLSRGR-LDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACVQADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHT-THKRIWEQKQNDVIASSHD--NTFASTRG---------EREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDR--DTAGGPLRLVDENKGVKNNPKWCQNPQYLLNIPARAQYESVDVKLVLRRQGDESHGRGKEASGSKPRRTSLTHNALTGLVICKARHPEDGAPLRRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQVAAASKDDQHSSSVLPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQ--TAGGSHIHPHWKKNPCFHLKLRTTGPAKVRISVSRPEKDWKNKCIRDSVGCMMGFYLMSGPKPNRD-HTIFHEGRPYEGSPMVPTDEVSTPPGFMLNTTSEDDVFTIMPATFEPGKTGPFFISIATTDVDFSLR 2799
            E  G T E ++IA L +      +YED  F     SL  N    PEY             D   S + W  P ++   P YF     AG G L  G  L+D+WLLG    LA HP  LIENLF S   DF  FG+YT  FYK+ +W EVVTDTR+P +     + ++   G  V      +YG  +N +E +VP+L KAYAK HG YE ++ GS+ EA VD TGG  +K+    +  + +IE G LW  + +HV    VV C     +   +  + TG+L NH Y +  +K          + V+++N W  G WKGDWS+  + WED  +V A + +DP   + R   DGTFWM++ DFV  F +LY+ R FP   ++QYC+ GEW G+ A G   PMK      ++  S         R+ +V+   D +P WF NPQYRL   E   V +SL+Q+D R      +N+ I F +++ K+       +WE  +  V+A  H   +  A+T           ERE+++ +V L P  AY  +P+      E +F  R+FS K V ++ +    T  + G W R  D     AGGPL + +   G++N   WCQNPQY+L   A +  + VD+KLVL+R G ++  +G      K +        L GL I K    E  A        P + N LGEPL     S  +P R +  +Q   A       ++ LP RK++V+  E+C +SDY S   +++ L+ +  +++  GL +VP+L +   +GAY +EVHSD  + ++E+P    +T+A EWN +  TAGGSH+ P WKKNP F+L L+   P  V I++ R E +W+ KC +DSVG MMGFYL  G K  RD  T+  +G+ +  +  VP   VS+P    L     +  + IMP T+EP K G F +S+ + D +F+L+
Sbjct:    4 EGAGITLERNVIAMLSECGDIPPMYEDPDFAAKPISLYLNPDKPPEYATSSKRPTANGGADEAASAIAWYRPGQVTADPDYFK--CTAGCGVLREGTGLNDSWLLGVFAALALHPDNLIENLFVSPMHDFKTFGIYTCQFYKDCQWLEVVTDTRLPYSQ-PLEEVNEPKTGSAVMRPGHWLYGSSVNKSELFVPLLMKAYAKFHGNYEVLHNGSILEAFVDCTGGSVKKIDLADDASRKLIETGQLWHKVVKHVHYKSVVTCQLKMASMAYNEVTGTGILKNHLYVVQYVKELG-----PLKFVKLKNVWQKGLWKGDWSNDDSKWEDNLQVEAALRADPLCEFNRTKADGTFWMMWEDFVEAFNELYIVRNFPST-FQQYCVRGEWVGQAAAGP--PMK--PPPSVETSSASAPSAPSARKWLVEP--DSEPSWFLNPQYRLVVTEKTSVVLSLLQRDFRVFGG--DNFGINFVLLEVKKRALASSMVWEFDKTAVVAEGHSYPSGAAATAALGDKSAGHPEREISKGNVVLEPDVAYIFIPYTDHGGVEMEFFFRVFSPKPVQIDSLTPLNTLIVQGRW-RVDDEGHSNAGGPL-VQNLTTGMENLT-WCQNPQYVLRSFAAS--KPVDIKLVLKRTGIKATAKGHRRDHQKDK------GQLIGLAIVKPDVDESAAQATCKKKHPEKTNFLGEPL----LSPTKPRRAQSVVQTKTAE------NATLPPRKLLVKADEYCVLSDYSSAHVASIFLRKVPPEWLAKGLLVVPSLGDARGEGAYDVEVHSDDVITMDEIPNEMVQTIAGEWNDKVNTAGGSHLCPDWKKNPKFYLTLKCVRPVSVTINLYRSEYEWRGKCKKDSVGAMMGFYLFQGTKGTRDTSTVVVDGKTWTETDFVPLHHVSSPKDLTLPPVFNES-YVIMPTTWEPNKCGRFLLSVES-DCEFTLQ 916          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A8J2SDL8_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SDL8_9STRA)

HSP 1 Score: 513 bits (1321), Expect = 1.370e-163
Identity = 354/969 (36.53%), Postives = 515/969 (53.15%), Query Frame = 1

Query:   67 EEGDIIARLQASQREN-LYEDGSFPPNRSSL--NTKSVPEYDADLSE-VKWLYPHEICEAPVYFSDDANAGSGYLSRGRLDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACVQADP-NRGTS-------MDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSH--GEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRR-----DSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHTTHK-RIWEQKQNDVIASSHDNTFASTRGEREVTRDSVKLNPRYAYNIVPHPM-EQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDTAGGPLRLVDENKGVKNNPKWCQNPQYLLNIPA------RAQYESVDVKLVLRRQGDESHGRGKEASGSKPRRTSLTHNALTGLVICKARHPEDGAPL-RRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQVAAASKD--------DQHSSSVLPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQTAGGSHIHPHWKKNPCFHLKLRT-TGPAKVRISVSRPEKDWKNKCIRDSVGCMMGFYLM-------SGPKPNRDHTIFH--------EGRPYEGSPMVPTDEVSTPPGFMLNTTSEDDVFTIMPATFEPGKTGPFFISIATTDVDFSLRRDRG 2811
            EE  +I +L+ S+ E+  Y D  F    ++L  + + VP YD++L E + WL P E+C  P YF D A   SG +  GR  D WLLGA+  L  HP  LIENLF S+PDDF  +GVYT  FY+ G W EV  DTR+P  +     G  A  G++ P GP  +YG  ++  EQWV +LEKAYAK+ G+YE+++ GSV EALVDLTGG  E++    +  K   +  +L+E    ++   +V+ CV ADP N G           +  GLL  HAYG+  M+   +      +LVR+RNPWS   G WKG W+D+S+ WED+PEV  E     +  W R     D  DG+F+M FSDF   F  L+LCR+F D+QY+QY +   W+GKTA G  + +   + +   K    K K             D D  WFNNPQ+R+        +ISL+Q   +S     E + +  EV++ K+      R+WE+   DV  S+  N        REV+  +V L P Y Y +V     E+ RE  F LR+FS +++ ++P+ E  +++L GSW+  ++ D+AGG     ++++ + N P + Q+PQY LN+P       +A    V+V LVLRR  D +  + ++A                 + +C  R P     L ++H     +VNALGE  + K S+LK+  R    L    A            + S +  P +KI + P EW ++S + +   + L L  L R   P+GL + P LSE+G+ G Y LE+H+D P  +E +  + S+T+A EW  + AGGSH+ P W  NP + ++    T   +VR+ ++RP + W +    D VG MMGFY+        +GP  + DH I H        E  PY  SP  P   V TP  F L+    +D F I+PAT+ PG+TGPFF+S+ T+DVDF+L   +G
Sbjct:    7 EEDAVIQQLRESRTEDEQYFDVHFGGLPAALYRDEEDVPPYDSNLDEPIVWLRPGELCRDPQYFVDGAIGVSGCVVEGRNGDGWLLGAMAALWGHPEYLIENLFGSDPDDFQEWGVYTCRFYREGSWVEVCADTRMPALS-----GFTAAEGEE-PTGPQCLYGRCVDPREQWVQLLEKAYAKVLGSYEALSKGSVTEALVDLTGGSGEEILLNEDSAK---KTSTLFEECQDYLENKHVL-CVTADPANNGQGGVPSPDLKQTALGLLPGHAYGV--MQCIQV---EDKKLVRLRNPWSGRGGTWKGPWADSSSQWEDFPEVLDECLKQASG-WTREEDGIDQTDGSFFMEFSDFCSKFDTLHLCRLFDDKQYKQYKVLSSWAGKTAAGPDEGVPVSTDTGFAKLIAAKAKPRT----------DKDACWFNNPQFRVEVKHKSTTHISLLQGPPKSG----ERFELCLEVIREKKTPALPGRVWER--GDVYRSTEVNQPVM----REVSLSNVVLEPGYTYCVVARAATERGREAPFALRVFSPRELVLQPLDECESSYLPGSWNGAAEVDSAGGAPFHFEKDQLIPN-PTFRQSPQYFLNLPEPPPDHPKATTGEVNVTLVLRRT-DPTPDKAQKAQ----------------IGLCVFRGPAPEVVLGKKHKKTGPKVNALGEKPKGKPSTLKKKRRQAAQLLDGLAGPQHGWDNDLRSEDSITTAP-KKIGLYPSEWHRLSSFGNETVAVLHLPTLVRSKFPHGLVITPCLSERGISGQYVLELHADCPTTLEMISASKSKTLAGEWTARHAGGSHLQPSWSTNPIYRVRFADPTARPRVRVDLTRPAEYWNSS---DVVGRMMGFYIHRADGPNGTGPSLDPDHCIMHVDPNLKDVEPEPYTQSPFTPLHTVQTPQAFRLD--KSEDPFLIVPATYAPGQTGPFFLSV-TSDVDFTLTAAQG 914          

BLAST of mRNA_P-lacustris_contig976.16074.1 vs. uniprot
Match: A0A6G0XCD7_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XCD7_9STRA)

HSP 1 Score: 512 bits (1318), Expect = 1.470e-163
Identity = 338/955 (35.39%), Postives = 508/955 (53.19%), Query Frame = 1

Query:   49 EEIGDTEEGDIIARL-QASQRENLYEDGSFPPNRSSL--NTKSVPEYDA------DLSEVKWLYPHEICEAPVYFSDDANAGSGYLSRGR-LDDTWLLGALGVLASHPGVLIENLFASEPDDFVRFGVYT--FYKNGEWQEVVTDTRIPCATCSKSKGDDALLGQKVPGGPTPIYGHGLNLNEQWVPMLEKAYAKLHGTYESINGGSVGEALVDLTGGCCEKMSFTSERVKPMIEDGSLWETLHRHVSPDYVVACVQADPNRGTSMDSDTGLLINHAYGLADMKACALYLCSGFRLVRMRNPWSHGEWKGDWSDASTLWEDYPEVYAEVSSDPNTPWRRDSNDGTFWMVFSDFVRLFTKLYLCRVFPDQQYRQYCIHGEWSGKTAGGSAKPMKRGSVSRLQKDSQDKMKDSLRRESVVDIQDDGDPYWFNNPQYRLSADEVVEVYISLMQQDRRSANNLRENYRIGFEVVQTKRHTTH-KRIWEQKQNDVIASSH---------DNTFASTRGEREVTRDSVKLNPRYAYNIVPHPMEQNREGKFTLRIFSRKDVCVEPIIETFTTHLHGSWDRTSDRDTA---GGPLRLVDENKGVKNNPKWCQNPQYLLNIPARAQYES---VDVKLVLRRQGDESHGRGKEASGSKPRRTSLTHNA-LTGLVICKARHPEDGAPLRRHVAAPRRVNALGEPLRTKESSLKRPTRLRPDLQVAAASKDDQHSSSVLPDRKIVVEPLEWCQMSDYRSTEASTLMLKNLRRDFMPNGLFLVPTLSEKGVKGAYSLEVHSDHPVEVEELPETSSRTMAAEWNTQ--TAGGSHIHPHWKKNPCFHLKLRTTGPAKVRISVSRPEKDWKNKCIRDSVGCMMGFYLMSGPKPNRDH-TIFHEGRPYEGSPMVPTDEVST----PPGFMLNTTSEDDVFTIMPATFEPGKTGPFFISIATTDVDFSLRRD 2805
            E  G T E ++IA L +  +   +YED  F     SL  + + +PEY        D  E+ W  P ++   P YF      G G L  G  L+DTWLLG    LA HP  LIENLF S   DF  +G+YT  FYK+ +W EVV DTR+P +   +   +    G   PG    +YG  +N +E +VP+L KAYAK HG YE ++ GS+ EA VD TGG  + ++   +    +IE G LW  L +HV    V+ C     +   +  + +G+L NH Y +  +K          + V+++N W  G WKGDWS+  + WED  +V A + SDP   + R   DGTFWM++ DFV++F ++Y+ R FP Q + QYC+ GEW G+ A G   P+K+        ++      +  R+    I  + +P WF NPQYRL   E   V ISL+Q+D R      +N+ I F ++Q K+ + H  ++WE  Q+ V+A +H         + T  +   EREV++ ++ L P  AY +VP+      E +F LR+FS K V ++ +    T  L G W R S+  +A   GGPL     +     N  WCQNPQYLL    R+  +S   VD+KLVL+R G ++  +G+       R      NA   GL I +     D +P+ +   +  + N LGE   TK  +                   +   +  LP RK++V+  E+C +SDY + + +++ML+ +  +++  GL +VP+L E  + GAY LEVHSD  + +EELP   ++T+A EW+ +  TAGGSH+ P WKKNP  +L L++   A+V+I + R E +W++KC +DSVG MMGFYL  G K NR+  T+  +G+ +  +  VP   ++     PP F       ++ + IMPAT+EP  +G F +++ T D +F+L+ D
Sbjct:    4 EGAGITLERNVIAMLSECGEVPPMYEDPDFAAKAISLYQDPEKLPEYATSAKRAVDDGEIHWYRPGQVTADPDYFK--CTFGCGTLREGTGLNDTWLLGVFAALALHPDNLIENLFVSPMHDFKTYGIYTCQFYKDCQWLEVVADTRLPYSQQLEDVNEPGTKGVARPGHW--LYGSSVNKSEVFVPLLMKAYAKFHGNYEVLHNGSILEAFVDCTGGSVKMINMMLDESHKLIETGQLWYKLVKHVHYKSVITCQLKMASMSYNEVTGSGILKNHLYIIQHVKELG-----PLKFVKLKNVWQKGLWKGDWSNDDSKWEDNLQVEAALRSDPLCEFNRTKIDGTFWMIWEDFVQVFNEIYIVRNFP-QTFNQYCVRGEWVGQAAAGP--PLKQ------HPEASPPAPPTTTRKWF--IAPESEPAWFLNPQYRLVVTEKTSVVISLLQRDFRVFGG--DNFGINFVLLQVKKRSLHASKVWEFDQDAVVAEAHSLPQQQQQANGTDKTHHPEREVSKGNIILEPNEAYILVPYTDHVGVEMEFFLRVFSPKPVQIDALRPLNTVLLQGKW-RVSEEGSATNAGGPLC---HSLAAAENLHWCQNPQYLL----RSMDDSGKPVDMKLVLKRTGYKATAKGR-------RDHQKDSNAQCIGLTIVRPDVQVDASPVVKKKPS-EKANFLGE---TKHKA------------------KEGGGNKALPSRKLLVKADEYCCLSDYANPQVASVMLQKIAPEWLDRGLLVVPSLGEGRIDGAYELEVHSDASLTLEELPNGLAQTIAGEWSDKKGTAGGSHLCPDWKKNPKVYLTLKSVRAAEVKIDLYRSEYEWRSKCKKDSVGSMMGFYLFQGTKLNRESSTVVVDGKTWTETDFVPLHHIAASLTLPPVF-------NESYVIMPATWEPNHSGRFLVAV-TADSEFTLQTD 891