mRNA_P-lacustris_contig951.15903.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: A0A6H5KRB1_9PHAE (Tr-type G domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KRB1_9PHAE)

HSP 1 Score: 1025 bits (2649), Expect = 0.000e+0
Identity = 570/797 (71.52%), Postives = 634/797 (79.55%), Query Frame = 3

Query:  153 QEPTVTREGITLDLGFSAFSVALPEHLPQDHFSRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGA-SQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSP-ATCWEETVALGVDTVAQELSRRARVPDRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLL---ERNADGKMTVAALRAGGTWGDVSLADIASLPFPLEAAFVKQTHLLGLVSDNSIADGDSDVCFASKNERGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRVTP-DGRAGSAVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPLKLMHQGYLREELGQHHRERVQQTRSADYPANNDDE-----DGAPAAINAATTSAEESVAREAHTETP-PLLREGVVDSVKDGTADGEPVVVAVVSGLFSLEEDIRAIAKMGMAVRTKNAGLVGRLRGPFGKLGKCKVEFVKGEGGSVQAGERVSVP 2507
            + P     GITLDLGFSAF+V LPEHLPQD +S LQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIG+AVMSSGA  +VVLNKVDLIPE ERK RLR  E++I AQLAGT+LS+ASFVSCAAAVGGEKTAAST  A S+SSKGR K  GV SG  G A GA+   K    VR G GD+ AE LT  T   P ++C EET A+G++ VA EL+RRARVP RRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQ+VELP+ RLQKKVKSMQMFRKPV +ARAGDRVGMCVASLDAKLMERGV+TTPGS+RPISAAIALVRKVPAF G+CLSG YCH+SLGHSTAMATATFFGA+ELK+GLL   E +ADG   + AL   G   ++SL +IASLPFP EAAFV+QTHL G+ S+NS A  D +VC    +ER  LQYCHL FHTPVITPPSSVVLGSRLD+ S+   K   A PT  T  G+A   EHCRIAFHGRLV L+  VDG RK  G A A+LEFGT+ GQLKLFTEKCKTGVVFRV P D   G AVEVFGKDLFKKETNMPPF+GM+LLTEGGS G LES FGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDP K MHQGYLRE L +H  +  +  R+AD P ++D +     +GAP    A TT  EE  A+E   E P P LR+GVVDSVK+G AD EPVV+A VSGLFS EEDIR +AK GM+VR +  GLVGRL+GPFGKLGKCKV F + EGG ++ GERV VP
Sbjct:   43 KNPQSRERGITLDLGFSAFTVPLPEHLPQDQYSHLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGEAVMSSGAAVIVVLNKVDLIPEQERKARLRQAEKKIAAQLAGTKLSSASFVSCAAAVGGEKTAASTPLAGSRSSKGRAKKAGVLSGNVGSAEGATRHQKGSKDVRPGGGDDPAEPLTGITPPRPPSSCSEETPAMGIEAVAGELTRRARVPSRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQLVELPSLRLQKKVKSMQMFRKPVFIARAGDRVGMCVASLDAKLMERGVVTTPGSVRPISAAIALVRKVPAFTGQCLSGAYCHVSLGHSTAMATATFFGASELKNGLLRLFEHSADGLQALEALGDAGQGQELSLNEIASLPFPSEAAFVQQTHLCGVGSENSTAGADDEVCV---DERPVLQYCHLTFHTPVITPPSSVVLGSRLDSMSSSLYKTEAAHPTEETIEGLAAVQEHCRIAFHGRLVSLSAGVDGTRKGSGDARAALEFGTQAGQLKLFTEKCKTGVVFRVAPNDQTGGGAVEVFGKDLFKKETNMPPFVGMLLLTEGGSVGHLESTFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPSKRMHQGYLREALERHPHQPTEGARTADNPMSDDKDHDNHDEGAPGPAAATTTGVEEPAAQETRQEPPRPHLRDGVVDSVKEGVADDEPVVIAFVSGLFSQEEDIREMAKRGMSVRIETGGLVGRLKGPFGKLGKCKVVFEREEGGYLRPGERVFVP 836          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: A0A835ZB77_9STRA (P-loop containing nucleoside triphosphate hydrolase protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZB77_9STRA)

HSP 1 Score: 395 bits (1014), Expect = 1.780e-122
Identity = 262/660 (39.70%), Postives = 355/660 (53.79%), Query Frame = 3

Query:  153 QEPTVTREGITLDLGFSAFSVALPEHLPQDHFSRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTWGDVSLADIASLPFPLEAAFVKQTHLLGLVSDNSIADGDSDVCFASKNE-RGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAP------------PNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVML--ADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRV----------------------TPDGRAGSAVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKL 2021
            + P     GITLDLGFSAF+V  P  +     S +QFTLVDCPGHASLIRTIIGGAQI+DMMLLVVDATKGMQ QT ECVVIG+  M+S  D V+VLNK DL+PE ER+ R+  ++  +  QLAGT+ + A F + AAA                                            PG       ++A+       ++PA   + T A GV  +A+ L    R P R  + P + A DHCF++RGQGT+LTGTVL+G V+V QM+ELP   + +KVKSMQ++R+PV  A+ GDRVG+CVA LDA L+ERGV   PG+LRP+ AA+A VR V  F G+C SGG  H+S GH+T MATAT+FGA E+ + L++R+         +   G     +L+DI  +       F++Q     L   +   D  +    A+  E R PLQ+  L F TPV  PP  +V+GS+LD  +              P +A+ A P+G  G G       CRI  +GR+V    A ++D AR                  L+L+T + + GV+FRV                           A    EVFG+DLF +++ +  ++GM++    G  GR+E  FG+AG+F+ARFP G + KVG  L
Sbjct:   60 KHPQSRERGITLDLGFSAFTVPAPAAVAALCDS-VQFTLVDCPGHASLIRTIIGGAQIIDMMLLVVDATKGMQTQTAECVVIGETTMASADDLVIVLNKADLMPEDEREARIARLQARMRRQLAGTKFARARFATVAAA--------------------------------------------PGXXXXXEGDSAQP------AAPADAKQHTSA-GVVELAELLRSLVRSPQRSDTGPFHMAVDHCFALRGQGTVLTGTVLSGAVEVGQMIELPELAITRKVKSMQVYRRPVQRAQQGDRVGVCVAGLDAALVERGVAAAPGALRPVRAAVAAVRAVKFFRGKCPSGGKLHVSTGHATVMATATYFGAAEMAAILVDRSPAQSEEQGTVPPPG-----ALSDIPPVRVDWRRDFLQQP---ALTRGSDRPDSQAAASAATSQEGRPPLQFALLQFQTPVFAPPGGLVIGSKLDGDAGAQPAGGGAQRGTVPARAAAAEPSGXXGAG------RCRICLYGRIVEAFGAGELDAAR------------------LRLYTTRERRGVIFRVGAAAGGXXXXXXXXXXXXXXXXXXXXXAAHVHEVFGRDLFGRDSRVAAYVGMIVEGAQGEVGRIEGTFGQAGKFRARFPLGIAAKVGTPL 635          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: B8C2Z6_THAPS (Selenocysteine-specific elongation factor SelB (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C2Z6_THAPS)

HSP 1 Score: 392 bits (1008), Expect = 1.600e-119
Identity = 304/855 (35.56%), Postives = 412/855 (48.19%), Query Frame = 3

Query:  177 GITLDLGFSAFSVALPEHLP-------------QDHFSRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPG-VRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTWGDVSLADIASLPFPLEAAFVKQTHLLGL---VSDNSIADGDSDVCFASKNERGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRVTPDGRAGS-----AVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPLKLMHQ-GYLREELGQHHRERVQQTRSADYPANNDDEDGAPAAINAATTSAEESVARE----AHTETPPLLREGVVDSVKDGTADGEPVVVAVVSGLFSLEEDI-RAIAKMGMAVRTKNAGLVGRLRGPFGKLGKCKVEFVKGEGGSVQAGERVSVPR*HTPTSDIRRNRRNSRQ*LCLLWASYQLFAAPS-VGGADSVLVNTETML 2654
            GITLDLGFSAF + LPEHL              Q  +  LQ TLVDCPGHASLIRTIIGGAQI+DM+LLVVDATKG+Q QT EC+VI +    +  + +VVLNKVDL PE+ER++R+R  E+++   L GT+  NA  V  +A VGGEK AA                       GG     G    L   +R  S D           S P+                        PDR      +FA DHCF ++GQGT+LTGT L+G  + N MVE P    QKK+K +QMFR+  ++ + GDR G+CV++ DAKLMERG+I +PG+++ I  AIA+VRKV  F G   SG   H+S+GH+T MAT TF+GA                           G   LA +  L F     F+ Q   L +   V +NS  + +SD   A +    PL +  L F TPV  P  S+V+GSRLD                       V    CR+AF GRLV                    E  T   +LK +T+K K G V R+    R          EVFG DLFKKETNM  F+G+++ T+ G  G ++S+FG +G+F+  FPAGT ++ GD L L+F++Y  DP K +HQ G L          R +     D P           A +    + +   A+     ++      ++ G VD +K          +++VSGLFS+ +DI +   +  +   T+  G+V    G FGK+GKC+  F   +G S + G +V V      T   R    N+   LC +  S      PS +    + + NT T L
Sbjct:   43 GITLDLGFSAFLLPLPEHLRREASNPQQEQMQLQQKYDLLQVTLVDCPGHASLIRTIIGGAQIIDMVLLVVDATKGIQTQTAECLVIAEM---TTRNLIVVLNKVDLFPENEREERIRMAEKKMRMSLRGTKFENAMMVGISACVGGEKVAA----------------------IGGTNNIHGLLNILQSQIRAPSRD-----------SKPS------------------------PDR-----FHFAVDHCFPIKGQGTVLTGTCLSGSARPNDMVEFPTLATQKKIKGLQMFRRKANIIQQGDRAGICVSNFDAKLMERGIIASPGTVKLIRGAIAVVRKVRFFKGGLHSGAKFHVSVGHTTVMATVTFWGAR--------------------------GSADLAGLPRLKFDWNQDFLHQDDYLEVLPSVDNNSNNNDESDK--AKEKGLPPLHWARLEFLTPVYCPIDSLVIGSRLDTE---------------------VNANTCRLAFSGRLV-----------------ERYEAKTDHNRLKSYTKKEKIGTVCRLGDPFRRSDDNKLVRYEVFGTDLFKKETNMSQFVGLLMETDRGDIGSIQSSFGTSGKFRVNFPAGTDIREGDPLYLRFKRYANDPKKAIHQDGSL---------PRARPGSRVDVPIKKKKSKSKNGAAHGGLATGDGGTAKNDVIGSNGSNDKDVKIGEVDKLKGDVLPNGKHTMSIVSGLFSMADDINKHKGRRVVCTTTEEEGVVD---GSFGKMGKCRCLFE--DGISAEVGSQVKV------TMISRNAISNALLILCCIAVSTTNAFTPSGIQTRATAITNTATTL 746          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: A0A1Z5J8H9_FISSO (Tr-type G domain-containing protein n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5J8H9_FISSO)

HSP 1 Score: 380 bits (977), Expect = 3.740e-116
Identity = 284/788 (36.04%), Postives = 398/788 (50.51%), Query Frame = 3

Query:  177 GITLDLGFSAFSVALPEHLPQDHF---SRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSR-RARVPDRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAF-NGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTW--GDVSLADIASLPFPLEAAFVKQTHLLGLVSDNSIADGDSDVCFASKNERGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRVTP-----DGRAGSAVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPLKLMHQGYLREELGQHHRERVQQTRSADYPANNDDEDGAPAAINAATTSAEESVAREAHTETPPLLREGVVDSVKDGTADGEPVVVAVVSGLFSLEEDIRAIAKMGMAVRTKNAGLVGRLRGPFGKLGKCKVEFVKGEGGSVQAGERVSV 2504
            G+TLDLGFS F + LPEHL Q HF    +LQ TLVDCPGHASLIRTIIGGAQI+DM+LL+VDA KG QAQT EC+V+      +    +V LNK D+   +ER+ +L   + ++  +L GTR ++A  +  AA VGGEK AA+            KT                               +A H T +            + L VD +  +L   R  V +     P YF+ DHCF +RG+GT+LTGTVL+G   VN  +E P+  + +K+KS+QMF++ V   + GDR G+CV++LDAKL+ERG++  PG++     AIALVRKVP + +G+ +     H+S+GH+T MAT TF+GA EL      + A+ K   ++ +   +W  GD  LA +  +PF  +  FV Q  LL  +    IA GD             L +  L F TPV  P  S+V+GSRLD               GGT          CR+AF GRL+                   +E  T   Q++L+TEK K GVV ++       D +     E+FG DLFKKETNM  F+GM + TE G  G ++S++G +G+F+  FP GT V+ G+ L L+F+++  DP K M Q                   S   P +     GA   +      A +   RE       L+  G + S+K          +A+VSG F+ E +I+   K+G  V   +    G + G FGK GKCKV F  G   S + G++  +
Sbjct:   57 GMTLDLGFSCFFLDLPEHL-QKHFPDKKKLQITLVDCPGHASLIRTIIGGAQIIDMVLLIVDAVKGWQAQTTECLVLAQL---TSPHLLVALNKADMFEPNEREKQLEAAKSKVRKRLKGTRFADAPMIGVAACVGGEKAAANAE--------ETKT-------------------------------SAVHETYQ------------MDLLVDLLKAKLPPPRRSVTNEAKEQPFYFSVDHCFPIRGRGTVLTGTVLSGFAAVNDGIEFPSLGMDRKIKSIQMFKRQVQHIQQGDRAGICVSNLDAKLLERGIVAAPGAVPLWKGAIALVRKVPYYTSGKLMCNSKFHVSVGHTTVMATLTFWGARELA----HQAAEAKNDASSNKLMSSWLGGDADLAGLPKIPFDFQQDFVFQDSLLETLD---IAPGDPQETL--------LHWALLDFQTPVYCPAHSLVIGSRLD---------VAVDSDGGTA-------SSCRLAFSGRLI-----------------ERVEPSTAMTQIRLYTEKQKVGVVSKLGDPHKRFDDQKVVCYEIFGADLFKKETNMKLFIGMKMETEQGDIGEIKSSYGTSGKFRLYFPGGTEVQEGEPLYLRFKRFMHDPTKSMQQ-------------------STALPPSRP---GARLEVENPKKEANKKKPREKGVN---LI--GELASIKGDVLANGKYSMAIVSGFFAPEINIKE--KVGTKVFIPSTQEEGSIVGGFGKAGKCKVAFELGI--SAKEGDKAEL 710          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: A0A7S2PGD4_9STRA (Hypothetical protein n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2PGD4_9STRA)

HSP 1 Score: 386 bits (992), Expect = 7.130e-116
Identity = 297/848 (35.02%), Postives = 414/848 (48.82%), Query Frame = 3

Query:  177 GITLDLGFSAFSVALPEHLPQDH--------------------------------------------------FSRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDRRASAP---LYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTWGDVSL---ADIASLP---FPLEAAFVKQTHLLGL--VSDNSIADGDSDVCFASKN--ERGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEK------CKTGVVFRVTPDGRAGSAVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPLKLMHQ-GYLRE-ELGQHHRERVQQTRSADYPANNDDEDGAPAAINAATTSAEESVAREAHTETPPLLREGVVDSVKDGTADGEPVVVAVVSGLFSLEEDI-RAIAKMGMAVRTKNAGLVGRLRGPFGKLGKCKVEFVKGEGGSVQAGERVSV 2504
            GITLDLGFSAF + LPE L  +                                                   +  LQ TLVDCPGHASLIRTIIGGAQI+DM+LLVVDATKGMQ QT EC+VI +    +  + +VVLNK+D+ PE+ER++R++  E++I   L GT+  +A  V  +A VGGEK AA   G                G+ GG TG+                 A     E  G++            +  +   L  + R P+R A       +FA DHCF ++GQGT+LTGT L+G  + N MVE P    QKK+K +QMFR+  ++ + GDR G+CV++ DAKLMERG I +PG+++ I  AIA+VRKV  F G+  SGG  H+S+GH T MAT TF+GA E+   +       +  +A+       G  SL   AD+A LP   F     F+ Q + L    VSD+ +    S V   SK+   + PL +  L F TPV  P  S+V+GSRLD                       V    CR+AF GRLV   D+                     G+LK +T+K      C+ G  +R + D +     E+FG DLFKKETNM  F+G+++ T+ G  G ++S+FG +G+F+  FPAGT +K GD L L+F++Y  DP K +HQ G L +  +G      V+           D++       N A                      G ++ +K    +     +A+VSGLF++ +DI +   +  +AV T+  G +    G FGK+GK KV F  G   S + G RV +
Sbjct:  208 GITLDLGFSAFLLPLPEQLRGEQQTVSNSNAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGSRSSNSNLDQKYDLLQVTLVDCPGHASLIRTIIGGAQIIDMVLLVVDATKGMQTQTAECLVIAEM---TTRNLIVVLNKIDMFPENEREERVQLAEKKILMALRGTKFEDAKMVGISACVGGEKVAAVGDGG---------------GLIGGGTGSV---------------TATNAHGEVAGTN-----------NIHGLLDVLQSQMRAPNRDARPSPERFHFAVDHCFPIKGQGTVLTGTCLSGSARPNDMVEFPTLATQKKIKGLQMFRRKANIIQQGDRAGICVSNFDAKLMERGTIASPGTIKLIHGAIAVVRKVRFFKGQLNSGGKFHVSVGHQTVMATVTFWGAREIARQVEREQESQQQQMASNGNAPILGKSSLGGTADLAGLPRLKFDWNQDFLHQDNYLESIPVSDDVLT---SQVVETSKDGDSQPPLHWARLEFLTPVYCPMDSLVIGSRLDTE---------------------VNANACRLAFSGRLVERYDERQDN-----------------GRLKSYTKKEKVGRCCRLGDPYRRSDDNKL-VRYEIFGTDLFKKETNMSQFVGLLIETDSGDVGSIQSSFGSSGKFRVHFPAGTGLKEGDSLYLRFKRYANDPRKAIHQDGTLPQARIGSRVDPPVKXXXXXXXXXXXDEQSSTK---NGAV---------------------GEIEKLKGDILENGKSPMAIVSGLFTMADDISKHKGRKVVAVTTEEEGSI---HGSFGKMGKVKVSFENGV--SAEVGSRVKM 940          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: K0RBX0_THAOC (Tr-type G domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RBX0_THAOC)

HSP 1 Score: 386 bits (991), Expect = 1.680e-115
Identity = 296/841 (35.20%), Postives = 405/841 (48.16%), Query Frame = 3

Query:  177 GITLDLGFSAFSVALPEHLP--------------------------------------QDHFSRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTWGDVSL---ADIASLP---FPLEAAFVKQTHLLGLVSDNSIADG-DSDVCFASKNERGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRVTPDGRAGS-----AVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPLKLMHQGYLREELGQHHRERVQQTRSADYPANNDDEDGAPAAINAATTSAEESVAREAHTETPPL---LREGVVDS-----------VKDGTADGEPVVVAVVSGLFSLEEDI-RAIAKMGMAVRTKNAGLVGRLRGPFGKLGKCKVEFVKGEGGSVQAGERVSV 2504
            GITLDLGFSAF + LPEHL                                       Q  +  LQ TLVDCPGHASLIRTIIGGAQI+DM+LLVVDATKG+Q QT EC+VI +    +  + +VVLNK+DL P +ER++RLR  E+++   L GT+  NA  V  +A VGGEK AA                                           G +AA  +   T +            G+  V Q   R      + +    +FA DHCF ++GQGT+LTGT L+G  + N +VE P    QKK+K +QMFR+  +V + GDR G+CV++ DAKLMERG++  PG++R I  AIA+VRK     G   SG   H+S+GH+T MAT TF+GA E+     E  A  + +V  +  G + G  SL   AD+A LP   F  +  F+ Q + L  + D++   G  S      ++E  PL +  + F TPV  P  S+V+GSRLD+                      V    CR+AF GRLV                    +  +  G+L+ +T+K K G V R+    + G        EVFG DLFKKETNM  F+G+++ T+ G  G ++S+FG +G+F+  FPAGT V+ GD L L+F++Y  DP K + Q                +T S   P                            H   PP     R G  D+           +KDG  +G    VA+ SGLFS+E+DI +   +  +A+ T   G V    G FGK+GKCKV F KG G S + G ++ +
Sbjct:  233 GITLDLGFSAFLLPLPEHLRKETMVRQEVSTGKSDATKKPKNAWNIEKVQKKETETELQQRYDLLQVTLVDCPGHASLIRTIIGGAQIIDMVLLVVDATKGIQTQTAECLVIAEM---TTRNLIVVLNKIDLFPANEREERLRQAEKKMRNALRGTKFENAQMVGISACVGGEKVAAV------------------------------------------GQDAAACVISSTNN----------IHGLLNVLQSQMRAPNRDTQPSPERFHFAVDHCFPIKGQGTVLTGTCLSGSARPNDLVEFPTLATQKKIKGLQMFRRKANVIQQGDRAGICVSNFDAKLMERGIVAAPGTVRLIRGAIAVVRKAGFSKGGVNSGAKFHISVGHTTVMATVTFWGAREIARQAEEHLAAQQQSV--VENGESHGKSSLGGSADLAGLPRLRFDWDQDFLHQDNYLDSLPDDTDWGGRSSHQPVGKQSEEMPLHWARIEFLTPVYCPMDSLVIGSRLDSE---------------------VNANACRLAFSGRLV-----------------ERYDAKSDNGRLRSYTKKEKVGTVCRLGDPYKRGDDGKIVRYEVFGTDLFKKETNMTQFVGLLIETDAGDVGIIQSSFGTSGKFRVSFPAGTEVREGDPLYLRFKRYANDPRKAIRQDGSVP---------AARTGSRVDPPAKXXXXXXXXXXXXXXXXXXXXXXXXXHVVRPPNGTGARPGTGDACDSEVTGEIAKLKDGPTEGGRPTVAIASGLFSMEDDIGKHRGRRVVALSTDEEGAVD---GSFGKMGKCKVLF-KG-GVSAEVGSKLKM 964          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: F0YCG3_AURAN (Putative selenocysteine-specific elongation factor, selB n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YCG3_AURAN)

HSP 1 Score: 375 bits (964), Expect = 2.590e-114
Identity = 260/649 (40.06%), Postives = 337/649 (51.93%), Query Frame = 3

Query:  159 PTVTREGITLDLGFSAFSVALPEHLPQDHFSR-----LQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTWGDVSLADIASLPFPLEAAFVKQTHLLGLVSDNSIAD--GDSDVCFASKNERGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRVTP---DGRAGSAV-EVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPLKLMHQ 2072
            P     G+TLDLGFS+FSV LP+ L      R     LQFTLVDCPGHASLIRTIIGG+QI+DMM+LV+D  KG+Q QT EC+VIG+   S   + ++VLNKVD+IPE ER   +    + I  QLA ++ +NA+ V+CAAAVGGEK AA+                                                      G++P+        LG++ + + L RRAR+P R    P +FA DHCF +RGQGT++TGT L G   VN +VELP   L+KKVKSMQMFRKPV     GDR G+C++ LDA  +ERG++  PGS+  I++A+ALVRKV  F G C +    H++LGH+T +ATA FFGA EL +    R    +   A                    F     +V Q  L          D  GD     +  N+  PLQ+C L F T V     ++V+GS L+  +                       + CRIAFHG+LV   + VDG             F T+   +KL+  K K G+V ++     D      V +V GKDLFKKETNM  F+GM L    G  GR+ SAFGK+G+FK  FP GT VK   KL L F+KY   P K MHQ
Sbjct:   47 PESKARGMTLDLGFSSFSVPLPDQLRAGVAGRFDEANLQFTLVDCPGHASLIRTIIGGSQIIDMMVLVMDVNKGIQTQTAECLVIGEITTS---ELIIVLNKVDVIPEAERDATVAKTTKRIRLQLASSKFANATIVTCAAAVGGEKRAAAA----------------------------------------------------LGTAPS--------LGLEGLVETLRRRARMPSRDVEGPFFFAIDHCFPIRGQGTVVTGTALRGACAVNDIVELPEVGLEKKVKSMQMFRKPVKKIVCGDRAGLCLSQLDAAAIERGIVAAPGSVPGINSALALVRKVRFFRGPCDTDRKFHVTLGHTTTLATAYFFGAAELAA----RPKPAEPPTA--------------------FDSSREYVFQEQLAASKRKKGADDDGGDGPGPGSDLNDE-PLQWCLLKFETRVRCQLDALVIGSNLEADAL---------------------ADKCRIAFHGKLV---ERVDGDA-----------FATK---VKLYKLKRKEGLVAKLGDPCVDVTGARVVKDVVGKDLFKKETNMAAFVGMKLQAATGEMGRVASAFGKSGKFKVDFPGGTLVKPNGKLYLVFKKYVFSPSKAMHQ 569          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: A0A7S2RBF0_9STRA (Hypothetical protein n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2RBF0_9STRA)

HSP 1 Score: 351 bits (901), Expect = 1.130e-106
Identity = 243/647 (37.56%), Postives = 329/647 (50.85%), Query Frame = 3

Query:  153 QEPTVTREGITLDLGFSAFSVALPEHLPQDHFS---RLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDRR--ASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTWGDVSLADIASLPFPLEAAFVKQTHLLGLVSDNSIA-DGDSDVCFASKNERGP-LQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQ-----LKLFTEKCKTGVVFRVTPD--GRAGSAVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRD 2051
            + P     GITLDLGFSAF   LPEHL +D      ++Q+TLVDCPGHASLIRTIIGGAQI+D MLLVVD TKG+Q QT EC+VIG+ + +   D VVVLNK+DLIP+ +R+ ++  V + +   L+ TR   +  +  +A VGG+  A                                          G G    E +  E          E   +G+D +   + R   +P+R   A+ PLYFA DHCF ++GQGT+LTGTVL G V     VE+P   L++KVKSMQMFRKPV  A  GDRV +C+ +L+AKL+ERGV+ + GS+  IS AIAL+RKV  F  +C S    H+++GHST+M   TFFG+ ELK                             DIA         +V +     L  D+S+  + D ++    K + GP LQ+  L F TPVI    S+V+GSRLD                       V  + CRIAF+GR+                   + ++  +P Q     L+++  K + G V R+     G  G    V GK++F KET M  F+ + + TE    GR+ES FGK G+F   FP G +V  G KLIL+ +KY  D
Sbjct:   52 KHPQSKERGITLDLGFSAFITPLPEHLNKDGAGLSDKIQYTLVDCPGHASLIRTIIGGAQIIDRMLLVVDVTKGIQTQTAECLVIGEILTN---DLVVVLNKIDLIPDEKREAKIEKVSKMLEGALSTTRFKGSPIIPVSAIVGGDGKA------------------------------------------GVGGSNVETVVVEG---------EHQVMGLDKLVDLIKRTTVLPERGDIANQPLYFAVDHCFPIKGQGTVLTGTVLNGSVSTGDTVEIPELLLERKVKSMQMFRKPVVHAEKGDRVALCLTNLNAKLLERGVVASAGSVPRISGAIALIRKVKYFGKKCPSKSKIHVTVGHSTSMCNVTFFGSKELK-----------------------------DIAK-------TYVGKYLPTDLGFDSSLDYEFDEELRGGGKAQGGPFLQWAFLEFETPVICQMGSMVIGSRLDAD---------------------VDTKSCRIAFYGRI-------------------TTQYLKKPSQDELKLLRIYKLKERVGNVERIVDKTVGSDGKVQNVIGKNMFSKETKMDYFINLQVHTEENEVGRIESTFGKTGKFNVFFPKGANVSKGSKLILRHKKYIFD 568          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: A0A2R5G8P6_9STRA (Selenocysteine-specific elongation factor n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5G8P6_9STRA)

HSP 1 Score: 337 bits (865), Expect = 1.940e-101
Identity = 240/636 (37.74%), Postives = 326/636 (51.26%), Query Frame = 3

Query:  153 QEPTVTREGITLDLGFSAFSVALPEHLPQDHFSRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDR--RASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELKSGLLERNADGKMTVAALRAGGTWGDVSLADIASLPFPLEAAFVKQTHLLGLVSDNSIADGDSDVCFASKNERGP-LQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRVT---PDGRAGSAVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPA-GTSVKVGDKLILKFRK 2039
            + P     GITLDLGFSAF+V  PE L      ++Q+TLVDCPGHASLIRTIIGGAQI+D M+LVVD TKG+Q QT EC+VIG+ +     D +VVLNK+DL+PE  R   +  V + I   L+ TR   +     +A VGG+  A                     G+  G+  A  Q       ++G           +TGSS           G++ + Q + R   +P+R   +  PLYFA DHCF ++GQGT++TGT+L G V V  MVE+P   L++KVKSMQMFRKPV  A  GDRV +C A+L+A  +ERG ITTPGS+   SAAI L+RKV  F   C SG   H+++GH+T +A  TFFG+ ELK             VAA   G         ++  L F  E  F+    L G                  K + GP LQ+  L F  PVI    + V+GSRLD                     M+V  + CRIAF+GR+           K P       E      +L+++  K + G V R+    PD   G   +V  + +F K+T+M  F+ + + T+ G+ GR++SAFGK G+F   F   G  VK GD+L L+ ++
Sbjct:   40 KHPQSKERGITLDLGFSAFTVEAPEQLADTGVKQVQYTLVDCPGHASLIRTIIGGAQIIDRMILVVDVTKGIQTQTAECLVIGEILTD---DLIVVLNKIDLLPEATRTASVERVTKAIEQALSSTRFKGSPIACISAIVGGDGKA---------------------GVGEGSAAAPPQPPATTVKQQG-----------QTGSSGIE------TQGLEDLVQLIRRTTVMPERGDASKEPLYFAIDHCFPIKGQGTVVTGTMLGGTVNVGDMVEIPQLGLERKVKSMQMFRKPVEHAAKGDRVALCFANLNADALERGTITTPGSVPSTSAAIVLLRKVRFFPLACKSGSKLHVTVGHATTVAKVTFFGSAELKE------------VAAQHLG--------KNLPMLAFDHEQEFLYDEELRG----------------GGKAKGGPFLQWALLNFDQPVICQAGATVIGSRLD---------------------MSVETKSCRIAFYGRVASQ------YTKTP----TEEELA----RLRVYRFKERHGAVDRIVDKKPD-EVGRVRDVVARSMFSKDTDMSKFINLTVETDAGATGRIQSAFGKTGKFNVHFGGDGAVVKKGDRLTLRHKR 562          

BLAST of mRNA_P-lacustris_contig951.15903.1 vs. uniprot
Match: A0A482S2W3_9ARCH (GTP-binding protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S2W3_9ARCH)

HSP 1 Score: 337 bits (864), Expect = 1.550e-98
Identity = 281/878 (32.00%), Postives = 420/878 (47.84%), Query Frame = 3

Query:  153 QEPTVTREGITLDLGFSAFSVALPEHLPQDH-----FSRLQFTLVDCPGHASLIRTIIGGAQIMDMMLLVVDATKGMQAQTMECVVIGDAVMSSGADTVVVLNKVDLIPEHERKDRLRHVEEEITAQLAGTRLSNASFVSCAAAVGGEKTAASTHGASQSSKGRRKTGGVRSGIAGGATGASGQDKCLPGVRRGSGDEAAEHLTEETGSSPATCWEETVALGVDTVAQELSRRARVPDRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKVNQMVELPACRLQKKVKSMQMFRKPVSVARAGDRVGMCVASLDAKLMERGVITTPGSLRPISAAIALVRKVPAFNGECLSGGYCHLSLGHSTAMATATFFGANELK--SGLL-------ERNADGKMT------VAALRAGGTWGDVSL-----ADIASLPFPLEAAFVKQTHLL---GLVSDNSIADGDSDVCFASKNERGPLQYCHLAFHTPVITPPSSVVLGSRLDNTSAPPNKASVAPPTGGTGRGMAVAPEHCRIAFHGRLVMLADDVDGARKAPGGAGASLEFGTRPGQLKLFTEKCKTGVVFRVTPDGRAGSAVEVFGKDLFKKETNMPPFLGMVLLTEGGSFGRLESAFGKAGRFKARFPAGTSVKVGDKLILKFRKYFRDPLKLMHQGYLREELG--------QHHRERVQ-QTRSADYPANNDDEDG----AP-------------------------------------AAINAAT--TSAEESVAREAHTE--TPPL------------LREGVVDSVKDGTADGEPVVVAVVSGLFSLEEDIRAIAKMGMAVRTKNAGLVGRLRGPFGKLGKCKVEFVKGEGGSVQAGERVSV 2504
            + P   + GITLDLGFS+F++ LP HL  +      +  LQFTLVDCPGHASLIRTIIGGAQI+DM++LVVDA KG+Q QT EC+VIG+    +  + ++ LNK+D I E +R +++    ++I+  LA ++  +A  +  +A VGGEK AA   G+S  S   ++T  V+S                                                +GV+ +  E+ +  ++P R  +AP Y++ DHCF ++G GT+LTGTVL+G V VN  +E+P  R  +KVKSMQMF KPV  A+ GDRVG+CV +LDAK +ER +   P S+  +S+A+ LV+KV  F   C SG   H+++GH T + T  FFGA EL+  S LL       E  A    T      + ++R      + SL          L FP++A ++ Q  +    GLV  +                  P Q+  + F   +  P  S+++GS+LD  ++    +                   CR+AF G L  +    DGA      A   L F      + ++ EK    V+     D R G  VEV G  L  + T++  ++GM +L   G  G + SA+G  G+F+ +F     + VGDKL+ ++++Y  +  + + Q    E++G        ++  E V+   +S +       E+G    AP                                     A+I+A+   T   ESV      +  +PPL            LREG V+S K    DGE ++ A++SG F +EE+++    +G  V T + G+ G +   FGKLGKCKV F  G   S+  GE V V
Sbjct:   49 KHPQSQQRGITLDLGFSSFTLPLPPHLAAEFGSSCPYDTLQFTLVDCPGHASLIRTIIGGAQIIDMIILVVDANKGIQTQTAECIVIGEI---TSDNMIIALNKIDTIAEEDRPEKIEKASKKISKVLAASKFPHAKIIPLSAVVGGEKVAAVVSGSSSHSAAAKQTA-VKS------------------------------------------------MGVEDLITEIVQTVKLPHRNINAPFYYSIDHCFPIKGHGTVLTGTVLSGSVSVNSHIEIPHLRAVRKVKSMQMFHKPVDYAKQGDRVGICVTNLDAKSIERSIACAPDSVPLVSSALCLVKKVRFFKMSCKSGMKFHITVGHETVIGTVVFFGAEELQQASQLLTLPDSSKEEGAIASTTPSVDKLLQSMRQYHATYNPSLHGNFQQGFPQLDFPVDAEYLYQDKIRDTEGLVYGHE-----------------PAQWALIDFQHTIHCPLGSLIIGSKLDADTSDSRSSGA---------------HMCRLAFFGPLKAIVYPADGAA-----AQHPLAFPLPALGVYIWKEKYCEVVMLS---DVRKGGCVEVVGWKLVSERTSIQTYVGMKVLAPTGQVGSIVSAYGADGQFRVKFKVPVQLVVGDKLMFRYKRYVYNKARTITQEVYVEDVGSQEGQAGLENEMEHVKIANKSKERKKTMIQEEGGSVSAPPIRDKTPAMCSTTILPSTPSSPMLALSSEPNPVIVGVASIDASNIATDQNESVTTPVSPDEASPPLPSETAVEEMEGELREGKVESTK---VDGEHLI-AIISGAFRMEENVKLY--VGSVVVT-STGVKGNVTASFGKLGKCKVMFAGGC--SLPTGEGVCV 825