mRNA_P-lacustris_contig118.1373.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: D7G421_ECTSI (Acyl-CoA thioesterase, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G421_ECTSI)

HSP 1 Score: 822 bits (2122), Expect = 2.820e-292
Identity = 433/590 (73.39%), Postives = 471/590 (79.83%), Query Frame = 3

Query:   51 MKAAQRTWAVRSIDALRIFERMRISRGATPAFRNRYINQGIMRITSSFGLATVRGSEAKRFRGSVPQVSASTHFMPTKEEEVLPGAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSEDPSFPLLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGS-PQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQ------NGAPTGLLRSVLPANATEASRIVAAMHRDRLQDAADEQP*ASAQGAHEYLGW*I*RITSPVKELLTDRC 1799
            MKAA+R W  R+ +   +  R      +   F N   +       +S+   TV  S A R  G +P VS+STH +P+KEEEVLPGAFKSPIT+ALWEIRR EM+FQEKRR+AGG  P  NL PVPPSLSRSAIHYPFSTDSFLREDYRNPGGL+RIGRILEDLDALAGNIAFKHCSEDPSFPLLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSS+VINMR        CSTS PG+CYVGDDPWAD D DSCWLDANFTFVARDRETGRSRAVSPLDLG DPLQDE GD SDGSP ERDPE+GWRALWR VHSHKSRQALERNAAKK            GAAAEGF+E+EA+RLKRE+EA+ LV+AGFPLLHLPALANPHAVLMSQT TSSTLICMPQHRNTANRIFGGFLMH+AYEVAFCAAF FGGSRPRFSEIDEVVFLQPVDVGDLVKMDA ILYTKEGKE GS PQACVEVLASVVRPET+DS+VSNRFNLTFSFPEL +G +   +      + +P  LLR+VLPANATEASRIVAA HRDRLQDAAD++      G            T+PV   L  +C
Sbjct:    1 MKAARRAWTTRNANVAGMLRRRANRVNSLAGFMNNNNSSP----GTSWAAGTVDASSAVRCYGRIPNVSSSTHIVPSKEEEVLPGAFKSPITQALWEIRRAEMEFQEKRREAGGRPPRENLRPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLVRIGRILEDLDALAGNIAFKHCSEDPSFPLLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSMVINMR--------CSTSAPGVCYVGDDPWADGDDDSCWLDANFTFVARDRETGRSRAVSPLDLGTDPLQDEPGDGSDGSPGERDPEHGWRALWRVVHSHKSRQALERNAAKK------------GAAAEGFLEVEAARLKREQEASTLVAAGFPLLHLPALANPHAVLMSQTLTSSTLICMPQHRNTANRIFGGFLMHRAYEVAFCAAFTFGGSRPRFSEIDEVVFLQPVDVGDLVKMDACILYTKEGKEAGSSPQACVEVLASVVRPETVDSKVSNRFNLTFSFPELASGVEEGNRPCADGFSQSPAALLRTVLPANATEASRIVAAKHRDRLQDAADDREGLPRGGQ-----------TAPVVMRLGGKC 555          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: A0A835Z9W3_9STRA (Putative acyl-CoA thioesterase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9W3_9STRA)

HSP 1 Score: 297 bits (761), Expect = 5.460e-89
Identity = 193/469 (41.15%), Postives = 260/469 (55.44%), Query Frame = 3

Query:  300 PGAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSEDPS------FPLLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECG-SPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAMHRDRL 1685
            P A+KSP+T+A W++R  +   QE    A   + + +  P P S SR +I Y FS +S LR  Y NP G IRI R+LEDLDALA  IA +HC+ + S      +P+LVTASVDRI +++ P L+ D+ L G +VWTGRSS+ I M       +  +            P       S WL+A FTFVAR R+TGRS  + PL L   P +DEA                            +++A++R    ++GG  A      G A     E EA+       A  L+  G  L  +P LA+P +VL+  T  SS ++CMPQ RNTANRIFGGFLM +A E+ F  A+   G+ P  + +DEVVF QPVDVGD++K+D+ +L T+ G   G SP+A VEVLA V+RP+     +SNRF+ TF FPEL A RD    +G P   L+ VLP++  EA R+V  M R  L
Sbjct:   13 PEAYKSPLTEAFWDLRSVDKQLQEVEELA---AKQRHAHPRPASASRKSITYDFSRNSMLRSAYANPWGFIRISRVLEDLDALAATIAMEHCATECSAHGVTFWPMLVTASVDRIEMKQQPMLERDLVLSGHIVWTGRSSMQILMHCAVAQDSTPAAEDAAATAAAAAP-------SPWLEARFTFVARSRDTGRSTPIPPL-LIETPKEDEA-------------------------FRHAQRAMDRKKLLRSGGGGATLAGANGTAPVSSPEAEAA-------AAALLREGRLLKAMPCLADPRSVLVGDTAQSSVIVCMPQERNTANRIFGGFLMGRALELGFATAYTTVGAVPFTAVVDEVVFHQPVDVGDILKLDSCVLQTRAGGALGRSPRASVEVLAHVLRPDKRTMHLSNRFDFTFKFPEL-ARRD----DGEPQRELQRVLPSDVDEAQRVVQVMQRQAL 433          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: W7TVW1_9STRA (Thioesterase superfamily n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TVW1_9STRA)

HSP 1 Score: 272 bits (696), Expect = 5.630e-80
Identity = 171/458 (37.34%), Postives = 236/458 (51.53%), Query Frame = 3

Query:  303 GAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVP--PSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSEDPSFPLLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAM 1670
            G FKSPI   LW  R    + +        I       PV   PS S++++ Y F+ + FL + Y NP G  R G++LED+DALAGN+AFKHC +D   PLLVTASVDRI L  +P+LD D+ + GRVVWTG SS+ I++        +    P                   W+ A+FTFVAR  +T ++ A++ L                       PE    A  R   S   ++   R   +KA G             EG   L++  +  E     L+S   PL+ +P LA+P  VL+  T  S++ I  PQ RN  NR+FGGFLM +AYE+A+   ++F GSRP F ++  V F  PVDVGDL++ DA++LYT  G++ GSP   V+V+AS+ RPET+ S +SN FN  F  P            G PT  +R VLPAN  +A R++  M
Sbjct:   48 GRFKSPIITHLWSQRATGKELE--------IDVRSPTEPVERTPSESQTSVTYEFTREPFLSQSYENPWGFFRKGKLLEDMDALAGNVAFKHCEKDGKVPLLVTASVDRIRLSSNPKLDRDMEMTGRVVWTGSSSMQIDLE----VHALPDAKP-------------------WISASFTFVARHPQTAKATAINKLK----------------------PES---AAERETFSAVEQKNQRRKTVRKAKG------------TEGSPSLKSGAVDVETMIKLLLSQARPLMEMPCLADPTCVLVDNTRASNSSIMQPQQRNMLNRVFGGFLMRRAYEIAYSTCYLFAGSRPEFFQVAHVDFTSPVDVGDLMRFDAAVLYTHTGQQTGSPHVHVQVVASICRPETVHSTISNTFNFVFRVP------------GRPT--VRKVLPANLDQAKRMIERM 423          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: A0A078JTC3_BRANA (BnaCnng69840D protein n=12 Tax=Brassiceae TaxID=981071 RepID=A0A078JTC3_BRANA)

HSP 1 Score: 268 bits (684), Expect = 1.100e-77
Identity = 176/464 (37.93%), Postives = 249/464 (53.66%), Query Frame = 3

Query:  300 PGAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSEDPSFP---LLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAMHRDR 1682
            PG + SP+T ALWE RR   +        G  +    L    PS SR++I Y FS+D  LRE YRNP   IR G+++EDLDALAG I+FKHCS         +LVTASVDRI+++R   +D D++++G V W GRSS+ + + QV  +  +C + P     + D   A+  S+S  L+ANFTFVARD +TG+S  ++ +                 +P     +  W+            +A ERN  +K               A+G  E E  +LK  E   +L++ G   L +PALA+  ++L+  T   ++LIC PQ RN   RIFGGFLM KA+E+AF  A+ F G  PRF E+D V F++PVDVG+ +++ + ILYT E      P   +EV+A V  PE   S VSNRF  TFS       R  A ++G     +R+V+P    EA R++  M  +R
Sbjct:   67 PGMYHSPVTNALWEARRNMFETPT----GGEDASRSELTAKTPSRSRTSILYKFSSDFVLREQYRNPWNEIRTGKLVEDLDALAGTISFKHCSSGDGSARSMILVTASVDRIIMKRPIRVDADLSIVGAVTWVGRSSMEMQL-QVIQSQDLCISFPFESVDMQDFADANHPSESVALEANFTFVARDAKTGKSTPINQV-----------------TPETEHEKMLWK------------EAEERNKLRKQ------------KRAQGKEEQE--KLKDLERLNDLLAEGRVFLDMPALADRDSILIKDTSHENSLICQPQQRNIHGRIFGGFLMRKAFELAFSNAYTFAGVSPRFLEVDRVDFIKPVDVGNFLRLKSRILYT-EATSSAEPLINIEVVAHVTSPELRSSEVSNRFYFTFSV------RPEAMKDGMK---IRNVVPGTEEEARRVIERMDAER 472          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: A0A6P6WRG3_COFAR (acyl-coenzyme A thioesterase 9, mitochondrial-like n=4 Tax=Coffea TaxID=13442 RepID=A0A6P6WRG3_COFAR)

HSP 1 Score: 270 bits (689), Expect = 5.350e-77
Identity = 191/496 (38.51%), Postives = 260/496 (52.42%), Query Frame = 3

Query:  207 VRGSEAKRFRGSVPQVSASTHFMPTKEEEVLPGAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCS--EDPSFPLL-VTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAMHRDRL 1685
            V  + + RF  S P + A  H M  K   + PG + SP+T ALWE R     F+     A        L   PPS SR++I Y FSTD  LRE YRNP   IR+G+++EDLDALAG I+FKHCS  +  + PLL VTASVD+IVLRR   +D D+T++G V W GRSSL I +   ++A                     S SDS  L ANFTFVARD  TG+S  V+ +              S  + RE+        LW         +A ERN  +K         +  G   +G    E ++   E     L++ G     +PALA+  ++L+  T   ++LIC PQ RN   RIFGGFLM +A+E+AF  A+ F G+ PRF E+D V FL+PVDVG+ ++  + +LYT E +    P   VEV+A V RPE   S VSN+F  TF+       R  A +NG     +R+V+PA   EA R++  M  +++
Sbjct:  182 VNSTMSPRFETSAP-IDAG-HSM-RKPISLWPGMYHSPVTNALWEARSSI--FERLNNVAADAPSPNELIAKPPSKSRTSIVYKFSTDYILREQYRNPWNEIRMGKLVEDLDALAGTISFKHCSSADSTTMPLLLVTASVDKIVLRRPIRVDTDLTIVGAVTWVGRSSLEIQLEVTQSAQE-----------------TSSQSDSLALTANFTFVARDSRTGKSAPVNQI--------------SPETEREK-------LLWE--------EAEERNQIRK---------KKRGEKRQGIDNDEETKRLNE-----LLAEGRVFCDMPALADRDSILIRDTYLQNSLICQPQQRNIHGRIFGGFLMRRAFELAFATAYSFAGAAPRFVEVDHVDFLKPVDVGNFLRFKSCVLYT-ELENPDKPMINVEVVAHVTRPEFRSSEVSNKFYFTFTV------RPDAIKNGLK---IRNVVPATEEEAKRVIERMDAEKM 602          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: Q6AWX1_ARATH (At2g30720 n=10 Tax=Camelineae TaxID=980083 RepID=Q6AWX1_ARATH)

HSP 1 Score: 264 bits (675), Expect = 1.270e-76
Identity = 172/463 (37.15%), Postives = 246/463 (53.13%), Query Frame = 3

Query:  300 PGAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSEDPSFP--LLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAMHRDR 1682
            PG + SP+T ALWE RR   +        G  + +  L    PS SR++I Y FS+D  LRE YRNP   IR G+++EDLDALAG I+FKHC  D S    +LVTASVDRI+++R   +D D++++G V W GRSS+ + ++ ++   T                  ++ S+S  L+ANFTFVARD +TG+S  ++ +                  P     ++ W+            +A ERN  +K               A+G  E E  +LK  E    L++ G   L +PALA+ +++L+  T   ++LIC PQ RN   RIFGGFLM KA+E+AF  A+ F G  PRF E+D V F++PVDVG+ ++  + +LYT E      P   +EV+A V  PE   S VSNRF  TFS       R  A ++G     +R+V+PA   EA R++  M  +R
Sbjct:   64 PGMYHSPVTNALWEARRNMFEIP-----TGDDASQSKLTAKSPSRSRTSILYKFSSDFVLREQYRNPWNEIRTGKLVEDLDALAGTISFKHCGGDSSARSMILVTASVDRIIMKRPIRVDTDLSIVGAVTWVGRSSMEMQLQVLQIQDT------------------NNSSESVALEANFTFVARDAQTGKSAPINQV-----------------VPETEHEKFLWK------------EAEERNKLRKQ------------KRAQGKEEHE--KLKDLERLNELLAEGRVFLDMPALADRNSILIKDTSHENSLICQPQQRNIHGRIFGGFLMRKAFELAFSNAYTFAGVSPRFLEVDRVDFIKPVDVGNFLRFKSRVLYT-EATSSAEPLINIEVVAHVTSPELRSSEVSNRFYFTFSV------RPEAMKDGLK---IRNVVPATEEEARRVIERMDAER 450          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: A0A6D2JD65_9BRAS (Uncharacterized protein n=7 Tax=Brassicaceae TaxID=3700 RepID=A0A6D2JD65_9BRAS)

HSP 1 Score: 264 bits (674), Expect = 2.470e-76
Identity = 175/472 (37.08%), Postives = 250/472 (52.97%), Query Frame = 3

Query:  282 KEEEVLPGAFKSPITKALWEIRRG--EMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSE---DPSFPLLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAMHRDR 1682
            K   + PG + SP+T ALWE RR   E    +   ++GG++ +       PS SR++I Y FS+D  LRE YRNP   IR G+++EDLDALAG I+FKHCS    +    +LVTASVDRI+++R   +D D++++G V W GRSS+ + ++ ++                     A++ S+S  L ANFTFVARD +TG+S  ++P+                 +P     +  WR            +A ERN  +K               AE   E E  +LK  E    L++ G   L +PALA+  ++L+  T   ++LIC PQ RN   RIFGGFLM KA+E+AF  A+ F G  PRF E+D V F++PVDVG+ ++  + +LYT E      P   +EV+A V  PE   S VSNRF  TFS       R  A ++G     +R+V+PA   EA R++  M  +R
Sbjct:   68 KPVSLWPGMYHSPVTNALWEARRNMFENPTGDDDSQSGGLAAKS------PSRSRTSILYKFSSDFVLREQYRNPWNEIRTGKLVEDLDALAGTISFKHCSSGNGNARAMILVTASVDRIIMKRPIRVDADLSIVGAVTWVGRSSMEMQLQVIQAQD------------------ANNPSESVALKANFTFVARDAKTGKSTPINPV-----------------TPESEHEKLLWR------------EAEERNKLRKQ------------KRAESKEEHE--KLKDLERLNELLAEGRVFLDMPALADRDSILIKDTSHENSLICQPQQRNVHGRIFGGFLMRKAFELAFSNAYTFAGVSPRFLEVDRVDFIKPVDVGNFLRFKSRVLYT-EATSSAEPLINIEVVAHVTSPELRSSEVSNRFYFTFSV------RPEAMKDGLK---IRNVVPATEEEARRVIERMDAER 462          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: UPI00053C3CB2 (acyl-coenzyme A thioesterase 9, mitochondrial n=1 Tax=Tarenaya hassleriana TaxID=28532 RepID=UPI00053C3CB2)

HSP 1 Score: 261 bits (667), Expect = 1.500e-75
Identity = 191/539 (35.44%), Postives = 273/539 (50.65%), Query Frame = 3

Query:  114 MRISRGATPAFRNRYINQGIMRITSSFGLATVRGSEAKRFRGSVPQVSASTHFMPTKEEEVLPGAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSEDPSFP---LLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAMHRDRLQDAADEQP*ASA 1721
            MRIS G   +   R I+ G  RI  S  +++   S    F G  P + A +     K   + PG + SP+T ALWE R    +     + AG +S +  L    PS SR++I Y FS+D  LRE YRNP   IR+G++LEDLDALAG I+FKHCS D       +LVTASVDRI+ ++   +D D++++G V W GRSS+ I ++  + +              GD       S S  L+ANFTFVARD +TG+S  ++P+                  P     ++ WR            +A ERN  +K                    + +  + K  +    L++ G   L +PALA+  ++L+  T   ++LIC PQ RN   RIFGGFLM KA+E+AF  A+ F G  PRF E+D V F+ PVDVG+ +++ + +LYT E      P   +EV+A V RPE   S+VSN F  TFS       R  A ++G     +R+V+P+   EA R++  M      DA  E P +S+
Sbjct:    1 MRISTGRLLSHSLRRIH-GRPRIMESRKMSS---SSESSFAGEDPPIDAGSSMR--KPISLWPGMYHSPVTNALWEARSKIFE-----KPAGDVS-QSELAARSPSRSRTSILYKFSSDFVLREQYRNPWNEIRLGKLLEDLDALAGTISFKHCSSDDGTTRPMILVTASVDRIIWKKPICVDVDLSIVGAVTWVGRSSMEIKLQVFQPS--------------GDT------SGSVALEANFTFVARDAKTGKSTPINPI-----------------LPETEHEKFLWR------------EAEERNKLRKQ--------------KRAETKEDHEKQKDLDRLNELLAEGRVFLDMPALADRDSILIKDTSNENSLICQPQQRNVHGRIFGGFLMRKAFELAFSNAYAFAGVAPRFLEVDRVDFITPVDVGNFLRLKSRVLYT-EANTSAEPLINIEVVAHVTRPELRSSQVSNTFYFTFSV------RPEAMKHGLR---IRNVVPSTEEEARRVLERM------DAETESPLSSS 448          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: UPI0005403031 (acyl-coenzyme A thioesterase 9, mitochondrial n=1 Tax=Beta vulgaris subsp. vulgaris TaxID=3555 RepID=UPI0005403031)

HSP 1 Score: 261 bits (667), Expect = 4.340e-75
Identity = 178/498 (35.74%), Postives = 261/498 (52.41%), Query Frame = 3

Query:  225 KRFRGSVPQVSASTHFMPTKEEEVLPGAFKSPITKALWEIR-----RGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHCSEDPSFP---LLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDSDSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSAGFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSF-PELVAGRDMAAQNGAPTGLLRSVLPANATEASRIVAAMHRDRLQD 1691
            K FR +   + A + F   +   + PG + SP+T ALWE R     +GE++ +  +     IS +G L    PS SR+++ Y FS+D  LRE YRNP   IR+G+++EDLDALAG I++KHCS+        LLVTASVD+I+L++  ++D D+TL+G V W GRSS+ + +  ++ +                   A   SD   L ANFTFVARD ETG+S  ++ +                 SP     +     LW    +    + ++R   K                     +++   + R  E   L++ G   + LPALA+  ++L+  T   S++IC PQ RN   RIFGGFLM KA+E+AF  A+ F G+ PRF E+D V F++PVDVG+ ++M +S+LYT E +    P   VEV+A V RPE   S +SN+F  TF+  PE       A +NG     +RSV PA+  EA R++  M  +   D
Sbjct:   69 KTFRDASEPIDAGSSFR--QPVSLFPGVYHSPVTNALWEARSKFFEKGEIEVENDK-----ISQDG-LVSKTPSQSRTSVEYKFSSDFVLREQYRNPWNRIRMGKLVEDLDALAGTISYKHCSKSIGITRPILLVTASVDKILLKKPIQVDADLTLVGAVTWVGRSSMEMQLEVLQPSQD-----------------ASQKSDPLALVANFTFVARDAETGQSAPINRI-----------------SPETEQEKL----LWEQAEARNKMRKVKRGEQKN--------------------KIDNQDIIRINE---LLAEGRVFVDLPALADRDSILIRDTCLQSSIICQPQQRNIHGRIFGGFLMRKAFELAFSTAYTFAGASPRFVEVDHVDFVKPVDVGNFLRMKSSVLYT-EVQNPDRPLINVEVVAHVTRPELRTSEISNKFYFTFTVCPE-------ATKNGLK---IRSVYPASEEEARRVIERMDAEHSND 486          

BLAST of mRNA_P-lacustris_contig118.1373.1 vs. uniprot
Match: A0A7N0UH67_KALFE (Uncharacterized protein n=3 Tax=Kalanchoe fedtschenkoi TaxID=63787 RepID=A0A7N0UH67_KALFE)

HSP 1 Score: 259 bits (663), Expect = 2.080e-74
Identity = 181/483 (37.47%), Postives = 258/483 (53.42%), Query Frame = 3

Query:  240 SVPQVSASTHFMPTKEEEVLPGAFKSPITKALWEIRRGEMDFQEKRRKAGGISPEGNLPPVPPSLSRSAIHYPFSTDSFLREDYRNPGGLIRIGRILEDLDALAGNIAFKHC-SEDPSFP--LLVTASVDRIVLRRHPELDCDITLMGRVVWTGRSSLVINMRQVKTAATMCSTSPPGMCYVGDDPWADSDS-DSCWLDANFTFVARDRETGRSRAVSPLDLGRDPLQDEAGDDSDGSPRERDPEYGWRALWRAVHSHKSRQALERNAAKKAGGRAAHALRWFGAAAEGFVELEASRLKREEEATNLVSA----GFPLLHLPALANPHAVLMSQTETSSTLICMPQHRNTANRIFGGFLMHKAYEVAFCAAFMFGGSRPRFSEIDEVVFLQPVDVGDLVKMDASILYTKEGKECGSPQACVEVLASVVRPETIDSRVSNRFNLTFSFPELVAGRDMAAQNGAPTGL-LRSVLPANATEASRIV 1661
            S P + A +     K   + PG + SP+T ALWE R   ++  +  +    +S +G L    PS SR++I Y FS+D  LRE YRNP   IR+G+++EDLDALAG I+FKHC +ED +    LLVTASVD+IVL++   +DCD+++ G V W GRSS+ I +   ++                     + DS DS  L ANFTFVARD  TG+S  ++P+              S  + RE+        LW+        +A ERN  +K                    +   ++L  + E TN +SA    G     +PALA+ +++L+  T   ++LIC PQ RNT  RIFGGFLM +A+E+AF  A+ F G+ PRF EID V FL+PVDVGD ++  + +L+T E ++   P   VEV+A V +PE   S VSN+F  TF              + AP GL +R+V+PA   EA R++
Sbjct:   82 SPPPIDAGSSIR--KPVSLWPGMYHSPVTNALWEARSNVLEKLDDAQSEA-VS-QGKLAMKTPSSSRTSIIYKFSSDPILREQYRNPWNQIRMGKVVEDLDALAGTISFKHCCNEDRTTRPLLLVTASVDKIVLKKPISVDCDLSISGSVTWVGRSSMEIQLEVTQSTEE------------------NQDSLDSVALVANFTFVARDSRTGKSAPINPI--------------SPETEREK-------LLWQ--------EAEERNIMRKK-------------------KXXENKLGLKNEDTNRLSALLAEGRVFCDMPALADRNSILIRDTCHQNSLICQPQQRNTHGRIFGGFLMRRAFELAFATAYAFIGAAPRFMEIDHVDFLRPVDVGDFLRYKSCVLFT-ELEDSSKPLINVEVVAHVTKPELRSSEVSNKFYFTFG----------VRPDSAPNGLKIRNVVPATEEEARRVL 483