mRNA_P-lacustris_contig106.543.1 (mRNA) Pleurocladia lacustris SAG_25_93

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: D8LC21_ECTSI (VPS11_C domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LC21_ECTSI)

HSP 1 Score: 1183 bits (3060), Expect = 0.000e+0
Identity = 730/1113 (65.59%), Postives = 775/1113 (69.63%), Query Frame = 2

Query:  458 KKYGVVVTLGDDSVNLPGIGNPLAAGGSGXXXXXXXXXXXXXXXXXXNGRSPCVLKFWAGADMGVCIRAVDVASQMQG----RWAT--GGAGG-RAGVPRLTAFAITPDASQVACGFSDGMACLLQGDLVRSPLLPPPAPLLILPGEDSPSAVTALHFSKGQQRRRSGGVSSSNPPQPKLLVGYDLDDKGGNGSMPSAPGFPSLGLLSYDTRTDAEPLVLDEIGCQEGCSAFGQSTQEMVVGREDGVFFFSSEDRGGAAGFEGKKQHVGCLWNYILVVSEDGRSGRHTINMYDMRNKLVAFHALLPQGQAVKRISCQGERYEPNAEATILGNRAGNGTGSAVAGNGGSIGGSGEGGLGIHPDGQEFVGQAQRARHLFQAGAPFQEEPVPDGHLAGLR*RPRRGRDSGDLSSYQTCFARSTLHPRRDVQRALLSFLPLKEQELSCHAYSFVRLATRARG-HARRPPVQEGGLRGGDAAVPVHHRAARPQLRHPPLPRRTEDRPAYVLPRGAARRRPGLV*PHHSPPQLPHQA*GCRKAGPFYTPF-------------EQYNNATAAAATTTAIP*QNHSRKRRESSP-----------GGRGSGRGXXXXGGANFDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDG-------GSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGLRATVQSSSLSGAGSRVAMELPTFMASELEKGGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGVMS 3679
            +KYGVVVTLGDDSVN P       AGG G                  NGRSPCVLKFWAGADMG+CIRAVDVA+QM      RWA+  GGAGG RA +PRLTAFAITPDASQVACGFSDGMA LLQGDLVRSP+LPPP PLLI PGE++PSAVTALHFSKGQQR R        PPQPKL+VGYDLD +GGNG MPS P FPSLGLLSYDTRTDAEPLVLDE GCQEGCSAFGQSTQEMVVGREDGVFFFSSEDRGGAAGFEGKKQHVGCLWNYILVVSEDGRSGRHTINMYDMRNKLVAFHALLPQGQ VKRISCQGERY+PNA     G   GNG G+ VAGNG S+GGSGE                         G  F    +     + LR R R      D SS      R  L+P           + +     S H  S +    R  G H  +    EG ++     +                    +  P+YV+ R    +R GL+    S  +  H A    +A   +T               E+ +     +  TT                           GG G G G     GANFDV TAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPF EAS+QLRRRGKPLVAALPEDTTGVLMALCTGRY S PDPAT XXXXXXXXXXXX        +TAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWA+AG G       G LA KKQRE EVMALLDNPRAGYD+DHALVLVQMLNFKPGQLYLYEKLYMT+LVLEHYVDTGDTRNMIRVCRKEG NN DLWVQVLSYLVNN  SS   Q+S                   XXXXXX    XX        GEEGRWDDVRELL L+ERDQVLSPL QVVSILSQN QLPLWVTRDFLPRYL++SA+AAAAD   A ELRESTN+MRAEIQGLRATVQSSS SG  SRVAMELPTFMASELEKGGSK LS SGLGGLGGEQAKIHDIKAAQARSALDHEQFFK LEEAADGFGQVASY GKGVMS
Sbjct:  114 RKYGVVVTLGDDSVNPPPQAEAGGAGGVGAG----------------NGRSPCVLKFWAGADMGLCIRAVDVAAQMPDGQHQRWASSGGGAGGARARLPRLTAFAITPDASQVACGFSDGMALLLQGDLVRSPVLPPPPPLLIQPGEENPSAVTALHFSKGQQRGRXXXXXXXXPPQPKLIVGYDLDKEGGNGGMPS-PDFPSLGLLSYDTRTDAEPLVLDEQGCQEGCSAFGQSTQEMVVGREDGVFFFSSEDRGGAAGFEGKKQHVGCLWNYILVVSEDGRSGRHTINMYDMRNKLVAFHALLPQGQGVKRISCQGERYDPNA-----GTNLGNGAGNVVAGNG-SVGGSGE-------------------------GLAF----ILTNRNSLLRLRER------DTSSKLELLFRKNLYP-----------MAISLAYASDHDVSEILGIYRMHGDHLYKKGDFEGAMQQYQCTI-------------------GQLDPSYVIRRFLDAQRIGLL---TSYLEALHDA---GQASSEHTTLLLNCHTKLKVLDVEKLDRFIHPSGDTTXXXXXXXXXXXXXXXXXXXXTALSHRLGGAGDG-GSSGAAGANFDVATAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFSEASKQLRRRGKPLVAALPEDTTGVLMALCTGRYSSMPDPATAXXXXXXXXXXXXNSKDKASPTTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWAAAGSGQRGGSSGGGLAVKKQREDEVMALLDNPRAGYDADHALVLVQMLNFKPGQLYLYEKLYMTELVLEHYVDTGDTRNMIRVCRKEGNNNPDLWVQVLSYLVNNVPSSGDIQESSPLPDNAGDGNPAGGKSEDXXXXXXDVSSXXGEGAG---GEEGRWDDVRELLALMERDQVLSPL-QVVSILSQNSQLPLWVTRDFLPRYLSTSAAAAAADXXXASELRESTNKMRAEIQGLRATVQSSSSSG--SRVAMELPTFMASELEKGGSKTLSSSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKGLEEAADGFGQVASYFGKGVMS 1125          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: A0A836CMH8_9STRA (VPS11_C domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CMH8_9STRA)

HSP 1 Score: 449 bits (1155), Expect = 9.690e-134
Identity = 360/1042 (34.55%), Postives = 491/1042 (47.12%), Query Frame = 2

Query:  746 FAITPDASQVACGFSDGMACLLQGDLVRSPLLPP-----PAPLLILPGEDSPSAVTALHFSKGQQRRRSGGVSS-----SNPPQPKLLVGYDLDDKGGNGSMPSAPGFPSLGLLSYDTRTD--AEPLVLDEIGCQEGCSAFGQSTQEMVVGREDGVFFFSSEDR----GGAAGFEGKKQHVGCL--WNYILVVSEDGRSGRHTINMYDMRNKLVAFHALLPQGQAVKRISCQGERYEPNAEATILGNRAGNGTGSAVAGNGGSIGGSGEGGLGIHPDGQEFVGQAQRARHLFQAGAPFQEEPVPDGHLAGLR*RPRRGRDSGDLSSYQTCFARSTLHPRRDVQRALLSFLPLKEQELSCHAYSFVRLATRARGHARRPPVQEGGLRGGDAAVPVHHRAARPQLRHPPLPRRTEDRPAYVLPRGAARRRPGLV*PHHSPPQLPHQA*GCRKAGPFYTPFEQYNNATAAAATTTAIP*QNHSR----KRRESSPGGRGS-----------GRGXXXXGGANFDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYY-SKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLR-EGGR-------------------AGPTVADTLLELLLREWA------SAGDGG--SLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGLRATVQSSSLSGAGSRVAMELP-TFMASELEKGGSKALSPSGLGG---LGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGV 3673
            FA++  A QVACGF+DG   L                  P P++I P  + PS + ALHF++ Q     GGV+       +  + + L   D      NGS     G     +LS D R     EPLVLDE GC  GC+A    TQE++VGR DGV+F++ EDR    GGAAGFEG K+ VGCL    +ILV S+D RS R  +N+YD+RNK VAFH LLPQ + V   +C G    P+A     GNRA                               +V  ++ A  L                         R RD+          A+  L  R+++    +S     + +++     +                ++G   G  A     H     +             P+YV+ R    +R G +  +                       E  + A  A+A  T +    +++    K+ ++   G G             RG     G  FDV TA+RVLKSAG++DHAA LARR G HDW+LR+QLER  PD++ ALAY++ LP   A   L+R GK LVA+LPEDTT +L+ALCTG Y  S                          + + +   EDF+HL+VD   WLR+FL  +++ EGGR                   A   V DTLLELLLR+WA       AG GG  +L+ K+  EAE+MALLD+PRA YD+DHALVL  ML F PGQLYLYEKL MT L+LEHY++TG+TRNM+R+C++EG  + DLW  VLS+LV+ A  +  D  +                                           +     E+L L+  +Q L PL +V+ IL +NP LPLWV RD+L  +++ +A   +   A   +LR ST +MR E+  LR    S+ +   G+   +  P  F +    +    A+  SG GG   + GE AK  DIK AQA+ A DHEQF+K+LEEA DGF QVA+  GKG+
Sbjct:  138 FAVSSSAQQVACGFADGTVLLXXXXXXXXXXXXXXXXXXPVPVVIRPPGEGPSGIGALHFAEPQGG--GGGVAGVCLFICHEEKAEQLSPADGAAGESNGSSKGQSG-----ILSCDARAGYACEPLVLDERGCAHGCAAVNPETQELMVGRGDGVYFYTHEDRQVLKGGAAGFEGHKEVVGCLVKGGFILVASQDPRSQRTAVNIYDLRNKFVAFHLLLPQSEGVVAAACGGGG--PDAGGDFEGNRA-------------------------------YVITSRGALLLL------------------------RERDTA---------AKLELLYRKNLYPVAISLAYASDYDVAAVMDMYRMYGDHL--------YKKGDFEG--AVQQYQHTIGHVE-------------PSYVIRRYLDAQRIGHLTQY----------------------LEALHAAQHASADHTTLLLNCYTKLKDVKKLDAFILGDGGXXXXXXXXXXXARGD----GPQFDVETALRVLKSAGYSDHAAALARRAGAHDWYLRVQLERPSPDYADALAYMSELPPSLAETFLQRHGKALVASLPEDTTALLVALCTGSYTGSYRQRRAGGQGPRDGGSGDTGVAAGEGTQSGRCRPEDFIHLFVDHSAWLRVFLQCIVKGEGGRGXXXXXXXXXXXXXXXAHRASAVVCDTLLELLLRDWARLAAEVEAGGGGGDALSEKRACEAEIMALLDSPRAEYDADHALVLALMLGFTPGQLYLYEKLNMTGLILEHYMNTGNTRNMLRICKREGAKSPDLWATVLSHLVSRARGTSADFKASCDEIQASKPSLDSCSSLLTARC--------------------QHSSYHEVLGLLAHEQALPPL-EVLDILRENPDLPLWVARDYLTGHVSHTAKELSRHEADIDDLRRSTAQMRREMAVLRT---SAGVQSGGTASCLHSPHQFTSFVCVQQMPAAVLGSGGGGSAAMSGEHAKYVDIKRAQAQRAGDHEQFYKDLEEATDGFAQVATCFGKGM 1033          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: A0A7S1XWN4_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XWN4_9STRA)

HSP 1 Score: 224 bits (570), Expect = 8.540e-60
Identity = 171/511 (33.46%), Postives = 249/511 (48.73%), Query Frame = 2

Query: 2198 ANFDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLM----ALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWA-SAGDGGSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGLRA---TVQSSSLSGAGSRVAMELPTFMASE--------LEKGGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEA--ADGFGQVASYLGKGVM 3676
            + FDV  A  +L  +G+ +HA +LA++ G H+ ++ IQL R  PD++ AL YIA LP  E  + L+R G+ L+   P +T G+LM     L      SK D                           +   E+ + L+ D P  L  FL  +     +  P VA+ LLEL L  W  S+G+  + +AKK     ++ LL++P   YD++HALV+ QM  F+ GQL L E+L +T+LVL+ + D GD    I++CR+ G    DLW +VL   V    +S  D  +                                        E+ RWDDV+++L L E+  +L P +QV+ ILS NP++PL V   FL R L  +    A D A  RELR  T + R EI+ LR    T Q+++ +  GS + + +  FM           L +G  + L  S       EQ K  DI  +Q ++ALDHEQFFKELE A  + GF  +A Y G+GV+
Sbjct:   19 SGFDVERATEMLHRSGYHEHALKLAKKAGAHEQYVSIQLNREAPDYNNALEYIAGLPPFEGLKFLKRHGRELLNYKPSETAGLLMHLFRKLIGSMKKSKDDRKNKMT-----------------QRKMEEHIEELLPLFADHPEELLKFLESLRESDEKTPPAVANPLLELYLGRWEQSSGEAKTWSAKK-----ILDLLEDPECMYDANHALVMTQMAQFRRGQLLLLERLRLTELVLQAHADDGDLAAQIKLCRRAGRGRPDLWSRVLRDAV---EASMKDAAAGGAGVDGSDDDDWDD-------------------------EDERWDDVKQVLDLCEQGDILPP-QQVLDILSINPEMPLKVVSPFLERLLTGAQDQIAMDKAEIRELRAVTTQTRGEIENLRTVSKTFQANTCAFDGSPLELPVIHFMCGHSYNLSGLSLPEGEHECLQCSR------EQRKWTDIYNSQVQAALDHEQFFKELEHAHGSQGFDVIAKYFGQGVV 472          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: A0A152A377_9MYCE (Vacuolar protein sorting-associated protein 11 homolog n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A152A377_9MYCE)

HSP 1 Score: 222 bits (566), Expect = 4.790e-56
Identity = 157/495 (31.72%), Postives = 246/495 (49.70%), Query Frame = 2

Query: 2204 FDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDGGSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGLRATVQSSSLSGAG-SRVAMELPT--FMASELEKGGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGVMS 3679
            FDV TAI+V + AG+ +HA  LA ++  H+W+L+I LE    +   AL YI +L F+EA + L++ GK LV+ LP++TT VLM LCT     +P P                       +  KAA E+F+H++V +  WL  FL Y++++   + P V +T+LEL LRE    G G     KK++  E    L NP+A +D DHA++L Q+ N+K G LYLYEKL + + ++E++++  D   +IR C+K G  + +LWV+ LSY       S   QD                                              +++ E+L  I+RD ++ PL  V+ ILSQN    L V ++++ R L+        D +  ++  + T +MR EI  LR+  +    S     +  ++LP+  F+ S        + +       G    +  +IK  Q+ SA+ H+QFFK L  + DGF  V+ Y GKG++S
Sbjct:  474 FDVETAIKVCRQAGYYEHALFLASKNNRHEWYLKILLE-DLSEHRKALHYIQTLEFEEADKNLKKYGKTLVSELPDETTRVLMKLCTDY---QPIPTNDLFGRPMTDTLSTVSSTVGLGTKLKAAPEEFIHIFVSQTEWLVKFLEYMIQQNNDS-PMVYNTMLELYLREDQQRGSGSD---KKEKAYE---FLTNPKAKFDQDHAMILAQVHNWKQGILYLYEKLELFNEIIEYHMENNDYEGLIRACKKYGVKDPNLWVRALSYF------SITKQDCK--------------------------------------------NEIVEVLANIDRDNLIPPLL-VIQILSQNKNTTLEVIKEYITRRLSQETLQIDKDYSQIKQFADETEKMRNEITDLRSNAKIFQQSKCNFCQSPLDLPSIHFLCSHSFHQRCLSENERECTICGPNNRRFLEIKKNQSDSAIQHDQFFKSLRTSPDGFTTVSEYFGKGILS 906          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: K3WLY6_GLOUD (Vacuolar protein sorting-associated protein 11 homolog n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WLY6_GLOUD)

HSP 1 Score: 223 bits (567), Expect = 9.980e-56
Identity = 239/922 (25.92%), Postives = 375/922 (40.67%), Query Frame = 2

Query: 1043 SLGLLSYDTRTDAEPLVLDEIGCQEGCSAFGQSTQEMVVGREDGVFFFSSEDRGGAAGFEGKKQHVGCLWNYILVVSEDGRSGRHTINMYDMRNKLVAFHALLPQGQAVKRISCQGERYEPNAEATILGNRAGNGTGSAVAGNGGSIGGSGEGGLGIHPDGQEFVGQAQRARHLF-QAGAPFQEEPVPDGHLAGLR*RPRRGRDSGDLSSYQTCFARSTLHPRRDVQRALLSFLPLKEQELSCHAYSFVRLATRARGHARRPPVQEGGLRGGDAAVPVHHRAARPQLRHPPLPRRTEDRPAYVLPRGAARRRPGLV*PHHSPPQLP--HQA*GCRKAGPFYTPFEQYN---------------NATAAAATTTAIP*QNHSRKRRESSPGGRGSGRGXXXXGGANFDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLER---------------SQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDGGSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEI---QGLRATVQSSSLSGAGSRVAMELPTFMA---------SELEKGGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGV 3673
            S G      RT    +VLDE G    CS       E+ VG+ D V+F+++EDR    GFEG+K+++    +Y+LV   D R GRH +N+YD++NK +AF+  L    +      +     PN+ A                                 P  +  + + +  RH+  + GA F    V  GH+  L        +    S  +  F       R+++    +S       +++    S + +      H  +    +G LR    A  + H      +R     +R  +  AY+     A          H+   L    +    +K   F    E  +               N T AA    A         +R+SS G   + +G       NFDV TAI VL    +  HA  LA++H EH W+L+IQL+R                +   + AL YI  L F EA   LR+ G+ LV  LPE TT +L  LCTG++   PD                       +S  K+   DF+HL+V     L+ FL Y++     +  ++ +TLLE++L E A          +++ E  VMA+LDNPR  YD DHAL+ +QM   K G+ YLY KL+M  ++++ +++  D +++I   RK G  + +LW   L Y        +G                                           GEE  W ++++LL LI+ + V+ PL QVV +LSQ+ +LP+ V + ++   L +       D    +  +  T +M+ EI    G     Q++        + +    FM          SE E+   + ++ S       +   I  +K    + A +HEQFF +LE AADGF  +A Y GKG+
Sbjct:  246 SAGASGLPPRT----VVLDERGVDTNCSCVNDEG-EIAVGQTDAVYFYTTEDRSVCFGFEGEKKYLHFFKHYLLVAHVDPR-GRHQVNVYDLQNKFIAFNWTLTNTTSKGPAGGRKPPIAPNSRA---------------------------------PGARFGLDEMEEIRHVVCEFGAIFVVSSV--GHVYRLT-------EKDTTSKLEILF-------RKNLYSIAISLAFSSNYDIN----SIMDIFRMYGDHLYQKGDYDGSLR--QYARTIGHVEPSYVIRRFLDAQRIHNLTAYL----EALHEKAFANAEHTTLLLNCYTKLKDVKKLDKFIQLDEIVDGKKNDLSGGDALSAVNGTGAAGDKLA---------KRDSSAGAETAQKGPGTAA-LNFDVETAISVLWE-NYPQHALTLAKKHEEHSWYLKIQLDRISYVESEDSVALSTHEKERVADALEYIKHLSFSEADSNLRKYGRTLVTHLPEPTTELLKQLCTGKFV--PD-----------------------NSKLKSDPGDFLHLFVSHRAQLKEFLQYIVEVETVSNTSIGNTLLEMVLSETAD-----EKRTEEEVEDAVMAILDNPRVKYDEDHALIHLQMRGVKKGKRYLYNKLHMYHMLVQFHIEENDDQSIIDEVRKHGEKDPNLWSLALKYFAERGPLPKG----------------------------------------ATTGEE--WRELKQLLALIDSNPVIPPL-QVVQVLSQSRELPVSVLKQYIVNQLLNDEKKIEEDEEKIKAFKSDTKQMKEEITQLSGKAVVFQATKCDLCNHDLDLPAVHFMCQHSFHLNCISETER---ECITCSM------DHRHIMGLKTQLEQKAGNHEQFFNQLETAADGFHTIAEYFGKGI 1009          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: A0A2D4BR03_PYTIN (RING-type domain-containing protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BR03_PYTIN)

HSP 1 Score: 213 bits (541), Expect = 4.360e-52
Identity = 259/1021 (25.37%), Postives = 398/1021 (38.98%), Query Frame = 2

Query:  737 LTAFAITPDASQVACGFSDGMACLLQGDLVRSPLLPPPAPLLILPGEDSPSAVTALHFSKGQQRRRSGGVSSSNPPQPKLLVGYDLDDKGGNGSMPSAPGFPSLGLLSYDTRTDAEPLVLDEIGCQEGCSAFGQSTQEMVVGREDGVFFFSSEDRGGAAGFEGKKQHVGCLWNYILVVSEDGRSGRHTINMYDMRNKLVAFHALLPQGQAVKRISCQGERYEPNAEATILGNRAGNGTGSAVAGNGGSI-----GGSGEGGLGIHPDGQEF-VGQAQRARHLF-QAGAPFQEEPVPDGHLAGLR*RPRRGRDSGDLSSYQTCFARSTLHPRRDVQRALLSFLPLKEQELSCHAYSFVRLATRARGHARRPPVQEGGLRGGDAAVPVHHRAARPQLRHPPLPRRTEDRPAYVLPRGAARRRPGLV*PHHSPPQLPHQA*GCRKAGPFYTPFEQYNNATAAAATTTAIP*QNHSRKRRESS--------PGGRGSGRGXXXXGGANFDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLER-----------SQPD-------------FSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDGGSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGL--RATV-QSSSLSGAGSRVAMELPTFMASELEKGGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGV 3673
            +TAFA++ D S +A G   G   L + DL R    PP    L+ P    P  VT L F+          +      +  L   +   D         A G P         RT    +VLDE G    CS       E+ VG  D V+F+++EDR    GFEG+K+++    +Y+LV   D R GRH IN+YD++NK +A                        +  T+ G   G G  S  A  G S      G +          G  F +   +  RH+  + GA F    +  G +  L        +    S  +  F       R+++    +S       +++    S + +      H       +G LR       + H      +R     +R  +  AY+     A          H+   L      C          +++       +   A+P     R +  +S        P    S  G    GG +FDV TAI VL    +  HA  LA++H EH W+L+IQL+R           SQ D              + ALAYI  LPF EA   LR+ G+ LV  LP  TT +L  LCTG++ S                          +++ K+   DF+ L+V     LR FL Y++       P + +TLLE++L +   A +         +E  V+A+LDNPR  YD DHAL+ +QM   K G+ YLY KL+M  ++++ +++  D ++++   RK G  + +LW   L Y        +GD                                             G W ++++LL+L++    + PL QVV +LSQ   LP+ V + ++   L+        D    R  +  T +M+ EI  L  +A V Q++        + +    FM          + +         +   I  +K    + A +HEQFF +LE AADGF  +A Y GKG+
Sbjct:  446 VTAFAVSDDISLLAVGLRXGAVILFRTDLKRRADRPPQ---LLQPAGQFP--VTGLAFASKPVTNTLAHIFLYASTRRGLTCYHCTHDDPALVKAAGASGMPP--------RT----VVLDERGVDVNCSCVNDDG-EIAVGXVDAVYFYTTEDRSVCFGFEGEKRYLQFFKHYLLVAHVDPR-GRHQINVYDLQNKFIA------------------------SNWTLTGASVGAGARSGGAAGGASSRPPLPGAASXXXXXXXAPGARFGLDDREEIRHVVCEFGAIFVVSSI--GCVYRLT-------EKDTTSKLEILF-------RKNLYSIAISLAFSSNYDIN----SIMDIFRMYGDHLYEKGDFDGSLR--QYVRTIGHVEPSYVIRRFLDAQRIHNLTAYL----EALHEKAFANAEHTTLLL-----NCYTKLKDVKKLDKFIQLEEGGSLVDAVPQPTPGRSQSIASVVSAAVPPPQPTESKTG----GGLSFDVETAISVLWE-NYPQHALTLAKKHEEHSWYLKIQLDRISYVEDPAGATSQADDGSTALSASEKERVADALAYITRLPFSEAEANLRKYGRTLVTHLPGPTTELLKRLCTGKFES-------------------------GNASLKSDPADFLXLFVSHREQLREFLQYIVEVETVTNPLIGNTLLEMVLSDATPADE---------KEEAVLAILDNPRVRYDEDHALIHLQMHGMKKGKRYLYNKLHMYHMLVQFHMEENDDQSILDEVRKHGDKDPNLWSLALQYFAERGPWPKGDTPRESG---------------------------------------GEWRELKQLLSLMDTITAIPPL-QVVQVLSQARDLPVSVVKQYVVNQLSQDEKKIEEDEEKIRAFKADTKQMKDEIAQLSSKAVVFQATKCDLCNHDLDLPAVHFMCQHSFHLNCXSETERECITCSLDHRHIMGLKTQLEQKAGNHEQFFNQLETAADGFHTIAEYFGKGI 1313          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: VPS11_DICDI (Vacuolar protein sorting-associated protein 11 homolog n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=VPS11_DICDI)

HSP 1 Score: 210 bits (535), Expect = 5.140e-52
Identity = 160/527 (30.36%), Postives = 254/527 (48.20%), Query Frame = 2

Query: 2204 FDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKP-DPATXXXXXXXXXXXXXXXXXXXXSSTA---------------------------KAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDGGSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGLRATVQSSSLSGA-GSRVAMELPT--FMASELEK----GGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGVMS 3679
            FDV TAI+V +  G+ D A  LA +H  HDW+L+I LE    ++  AL YI +L ++EA + L++ GK LV+ +PE+TTGVLM LCT     +  D  T              XXXXXX                               K+A E+F+H++V +  WL  FL Y++++G      + +TLLEL LR+  +  D   +    +R+A+    L NP++ +D DHAL+LVQ+ N+K G LYLYEKL + + ++E++++  D   +I+ C++ G  + +LWV+ LS+   N    +                                                  D++ E+LT I+++ ++ PL  V+ ILSQN    L V +D++ R L+        D    R+  + T +MR EI  LR   +    +      +A++LP+  F+          G ++   PS      G   +I +IK +QA SA  H+QFFK L  + DGF  V+ Y G+G+++
Sbjct:  486 FDVETAIKVCRQGGYFDRALFLASKHSRHDWYLKILLEDLN-EYRKALDYIQTLDWEEADKNLKKYGKQLVSEIPEETTGVLMKLCTNYQPVQAFDSLTALNLNGLTISNQTTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGFKQKSAPEEFIHIFVSQADWLVKFLEYMVQQGNNESSLIYNTLLELYLRDDVNQTDDERI----KRKAKAYEFLTNPKSKFDQDHALILVQVHNWKEGVLYLYEKLELFNEIIEYHMENNDYDGLIKACKRYGVKDPNLWVRALSFFSTNKQDCQ--------------------------------------------------DEIIEVLTNIDKENLIPPLL-VIQILSQNKNTTLAVIKDYISRRLSQETQQIDKDYTQIRQYADETEKMRHEINELRTNSKIFQQTKCIACLLALDLPSVHFLCQHSFHQRCLGENERECPS----CAGANKRIQEIKRSQADSANQHDQFFKLLRSSPDGFTTVSEYFGRGILN 952          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: A0A8J4PRN4_9MYCE (Vacuolar protein sorting-associated protein 11 homolog n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PRN4_9MYCE)

HSP 1 Score: 207 bits (527), Expect = 3.450e-51
Identity = 153/495 (30.91%), Postives = 241/495 (48.69%), Query Frame = 2

Query: 2204 FDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDGGSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGLRA---TVQSSSLSGAGSRVAMELPTFMASELEKGGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGVMS 3679
            FDV TAI+V +   + +HA  LA ++  H+W+L+I LE  + ++  AL YI +L F+EA + L++ GK LV+ LPE+TT VLM LCT   Y     A                      +  KAA E+F+H++V +P WL  FL Y++++   A P V +TLLEL LRE  S        +K + +A     L NP++ +D DHAL+L Q+ N+K G LYLYEKL + + ++E++++  D   +I+ C+K G  + +LWV+ LSY       S   QD                                              +++ E+LT I+++ ++ PL  V+ ILSQN    L V +D++ R L+        D +  +   E T +MR EI  LR    T Q +      S + +    F+            +         +  +I +IK +Q+ SA  H+QFFK L+ + DGF  +A Y G+ +++
Sbjct:  471 FDVETAIKVCRQGAYYEHALYLASKNNRHEWYLKILLEDLE-EYRKALHYIQTLEFEEADKNLKKYGKILVSELPEETTMVLMKLCTN--YQPIASANVFDLNALTLSQNDNGS----KNKVKAAPEEFIHIFVSQPEWLVKFLEYMVQQNNDA-PLVYNTLLELYLREDKSIPKTDEEKSKSKDKA--YEFLINPKSKFDQDHALILAQVHNWKKGVLYLYEKLELYNEIIEYHMENNDYEGLIKACKKYGIKDPNLWVRALSYF------SITKQDCQ--------------------------------------------NEIVEILTNIDKENLIPPLL-VIQILSQNKNTTLSVIKDYISRRLSQETQQIDKDYSLIKHFAEETEKMRNEINELRTNAKTFQQTKCIVCQSPLDLPSIHFLCQHSFHQRCLGDNERECPVCAPDNKRILEIKKSQSESAGQHDQFFKLLKTSPDGFSTIADYYGRSIIN 904          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: A0A8K1CGT8_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CGT8_PYTOL)

HSP 1 Score: 201 bits (511), Expect = 6.960e-49
Identity = 154/521 (29.56%), Postives = 241/521 (46.26%), Query Frame = 2

Query: 2192 GGANFDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLER---------------SQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTAKAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDGGSLAAKKQREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGL--RATV-QSSSLSGAGSRVAMELPTFMA---------SELEKGGSKALSPSGLGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGV 3673
            GG NFD+ TAI VL    +  HA  LA++H EH W+L+IQL+R                +   + AL YI  L F EA   LR+ G+ LV  LP  TT +L  LCTG++ ++                         +   K+   DF+HL+V     L+ FL Y++       P + +TLLE++L + A   D  +  ++ ++E  VMA+LDNPR  YD DHAL+ +QM N K G+ YLY KL+M  ++++ +++  D ++++   RK G  + +LW   L Y        +G                                           GEE  W ++++LL LI+ + V+ PL QVV +LSQ+ +LP+ V + ++   L +       D    +  +  T +M+ EI  L  +A V Q++        + +    FM          SE E+   + ++ S       +   I  +K    + A +HEQFF +LE AADGF  +A Y GKG+
Sbjct:  560 GGLNFDIETAISVLWE-NYPQHALTLAKKHEEHSWYLKIQLDRISYVESEDSMALSAKEKERVADALEYIKRLSFSEADANLRKYGRTLVTHLPGPTTELLKRLCTGKFVAE-------------------------NPKLKSDPADFLHLFVSHREQLKEFLQYIVEVETVTNPLIGNTLLEMVLTDEAEGDD--AKRSESEKEDAVMAILDNPRVRYDEDHALIHLQMHNMKKGKRYLYNKLHMYHMLVQFHIEENDDQSILDEVRKHGEKDPNLWSLALKYFAERGPLPKG----------------------------------------VTTGEE--WKELKQLLVLIDTNPVIPPL-QVVQVLSQSRELPVAVLKQYIVNQLLNDEKKIEEDEEKIKAFKTDTKQMKEEITQLSSKAVVFQATKCDLCNHDLDLPAVHFMCQHSFHLNCISETER---ECITCSL------DHRHIMGLKTQLEQKAGNHEQFFNQLETAADGFHTIAEYFGKGI 1000          

BLAST of mRNA_P-lacustris_contig106.543.1 vs. uniprot
Match: F0ZDJ5_DICPU (Vacuolar protein sorting-associated protein 11 homolog n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZDJ5_DICPU)

HSP 1 Score: 199 bits (506), Expect = 1.750e-48
Identity = 154/528 (29.17%), Postives = 242/528 (45.83%), Query Frame = 2

Query: 2204 FDVVTAIRVLKSAGHADHAAELARRHGEHDWFLRIQLERSQPDFSGALAYIASLPFDEASEQLRRRGKPLVAALPEDTTGVLMALCTGRYYSKPDPATXXXXXXXXXXXXXXXXXXXXSSTA--------------------------------KAAAEDFVHLYVDEPRWLRIFLTYVLREGGRAGPTVADTLLELLLREWASAGDGGSLAAKK-QREAEVMALLDNPRAGYDSDHALVLVQMLNFKPGQLYLYEKLYMTDLVLEHYVDTGDTRNMIRVCRKEGTNNLDLWVQVLSYLVNNASSSRGDQDSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEGRWDDVRELLTLIERDQVLSPLRQVVSILSQNPQLPLWVTRDFLPRYLASSASAAAADAAAARELRESTNEMRAEIQGLRATVQS-SSLSGAGSRVAMELPTFMASELEKGGSKALSPSG--LGGLGGEQAKIHDIKAAQARSALDHEQFFKELEEAADGFGQVASYLGKGVMS 3679
            FDV TAI+V +   + + A  LA +H  H+W+L+I LE    ++  AL YI +L ++EA + L++ GK LV+ +PE+TTGVLM LCT        P                      + T                                 K++ E+F+H++V +  WL  FL Y++++   + P V +TLLEL LRE     D    A +K +R  +    L NP++ +D D AL+LVQ+ N+K G LYLYEKL + + ++E++++T D   +I+ C+K G  + +LWV+ LS+       S   QD                                              ++++E+L  I+++ ++ PL  V+ ILSQN    L V +D++ R L         D    R+  E T +MR EI  LR   +          +  ++LP+           + LS +        G   +I DIK +QA SA  H+QFFK+L    DGF  ++ Y GKGV++
Sbjct:  473 FDVETAIKVCRQGNYFERALFLASKHNRHEWYLKILLEDLH-EYRKALDYIQTLEWEEADKNLKKYGKQLVSEIPEETTGVLMKLCTNY-----QPVQAFDSLSNLKFGFDNLKLNQQTQTQQHPNGNNSXXXXXXXXXXXXXXXXXXXXXXLEKSSPEEFIHIFVSKSEWLVKFLEYMVQQNIES-PAVYNTLLELYLRE-----DPNLTAEEKVKRVDKAYDFLTNPKSKFDQDQALILVQVHNWKKGVLYLYEKLELFNEIIEYHMETNDYAGLIKACKKYGVKDPNLWVRALSFF------SITKQDCQ--------------------------------------------EEIKEVLANIDKENLIPPLL-VIQILSQNKNTTLEVIKDYISRRLFQETQQIDKDYQQIRQYAEETEKMRHEINELRTNSKIFQQTKCVACQSPLDLPSIHFLCQHSFHQRCLSENERECPVCAGPNKRIQDIKKSQAESASQHDQFFKQLRSTNDGFTTISEYFGKGVLN 937