mRNA_P-wetherbeei_contig10373.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig10373.1.1
Unique NamemRNA_P-wetherbeei_contig10373.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: D7FWC5_ECTSI (Similar to Cytochrome b5 reductase 4 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWC5_ECTSI)

HSP 1 Score: 129 bits (325), Expect = 3.850e-36
Identity = 59/88 (67.05%), Postives = 71/88 (80.68%), Query Frame = 1
Query:    1 MRVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFL--EEEGEKASGD 258
            MR I+ AEVRQH ++HDCW++FRGKVYN+ PFL YHPGG PE+MKGAGRDCT LF+KYH WVN + L+ NL LG L  E+E E +SGD
Sbjct:    1 MRGITKAEVRQHNTQHDCWSIFRGKVYNLTPFLHYHPGGIPEIMKGAGRDCTALFDKYHRWVNFDGLVGNLYLGPLVTEKELEASSGD 88          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: A0A812XSF5_SYMMI (CYB5R4 protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812XSF5_SYMMI)

HSP 1 Score: 116 bits (290), Expect = 2.750e-31
Identity = 46/81 (56.79%), Postives = 63/81 (77.78%), Query Frame = 1
Query:    1 MRVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLEEEGE 243
            +R IS  E++QH +++DCWT   GKVYN+  +LAYHPGG+P+LM GAG+DCT LFNKYHPWV G+S+L+   +G L E+ +
Sbjct:    3 LRAISREELKQHNTQYDCWTALNGKVYNITQYLAYHPGGEPKLMLGAGKDCTALFNKYHPWVAGDSMLSKCQVGVLSEQSD 83          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: A0A835ZLC4_9STRA (Cytochrome b5-like heme/steroid binding domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZLC4_9STRA)

HSP 1 Score: 116 bits (290), Expect = 1.600e-30
Identity = 49/86 (56.98%), Postives = 64/86 (74.42%), Query Frame = 1
Query:    1 MRVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLEEEGEKASGD 258
            MR I+ AEVRQH+SEHD W+++ G+VYN+ PFL YHPGG   LM+GAG D T LFNKYH WVN ++++ NL LG L ++G +   D
Sbjct:   55 MRTITKAEVRQHRSEHDAWSIYEGRVYNITPFLHYHPGGVEMLMRGAGVDMTGLFNKYHRWVNAQNMIGNLCLGVLAKDGARGDDD 140          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: UPI000D153F64 (cytochrome b5 domain-containing protein RLF-like isoform X2 n=1 Tax=Physcomitrium patens TaxID=3218 RepID=UPI000D153F64)

HSP 1 Score: 113 bits (283), Expect = 2.830e-30
Identity = 47/76 (61.84%), Postives = 60/76 (78.95%), Query Frame = 1
Query:    4 RVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLE 231
            R+I M EV+QHK+E D WTV RG+VYN++P++ +HPGGK  LMKGAGRDCT LFNKYH WVN E L+   ++G L+
Sbjct:   35 RLIPMEEVKQHKTEEDAWTVLRGRVYNISPYIRFHPGGKDMLMKGAGRDCTALFNKYHVWVNAEFLMEKCLVGILD 110          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: A0A2K1IWB2_PHYPA (Cytochrome b5 heme-binding domain-containing protein n=4 Tax=Physcomitrium patens TaxID=3218 RepID=A0A2K1IWB2_PHYPA)

HSP 1 Score: 113 bits (283), Expect = 3.970e-29
Identity = 47/76 (61.84%), Postives = 60/76 (78.95%), Query Frame = 1
Query:    4 RVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLE 231
            R+I M EV+QHK+E D WTV RG+VYN++P++ +HPGGK  LMKGAGRDCT LFNKYH WVN E L+   ++G L+
Sbjct:  131 RLIPMEEVKQHKTEEDAWTVLRGRVYNISPYIRFHPGGKDMLMKGAGRDCTALFNKYHVWVNAEFLMEKCLVGILD 206          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: D5ADU0_PICSI (Cytochrome b5 heme-binding domain-containing protein n=1 Tax=Picea sitchensis TaxID=3332 RepID=D5ADU0_PICSI)

HSP 1 Score: 112 bits (280), Expect = 8.940e-29
Identity = 47/76 (61.84%), Postives = 61/76 (80.26%), Query Frame = 1
Query:    4 RVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLE 231
            R+ISM EV+QHKSE D WTV +G+VYN++P++ +HPGG+  LMK AG+DCT LFNKYH WVN E LL   ++GFL+
Sbjct:  124 RLISMDEVKQHKSEGDIWTVLKGRVYNISPYMKFHPGGEDMLMKAAGKDCTALFNKYHAWVNAEFLLEKCLVGFLD 199          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: D8SX26_SELML (Cytochrome b5 heme-binding domain-containing protein n=2 Tax=Selaginella moellendorffii TaxID=88036 RepID=D8SX26_SELML)

HSP 1 Score: 108 bits (271), Expect = 1.990e-28
Identity = 46/77 (59.74%), Postives = 59/77 (76.62%), Query Frame = 1
Query:    4 RVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLEE 234
            R+I+M EV+QH+ + D WTV RG+VYN+ P+L +HPGG   LMK AG+DCT+LFNKYH WVN E LL   +LG LE+
Sbjct:   41 RMIAMEEVKQHRKQDDAWTVLRGRVYNITPYLNFHPGGLDMLMKAAGKDCTSLFNKYHAWVNAEFLLERCLLGALEQ 117          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: A0A830CAD9_9LAMI (Cytochrome b5 domain-containing protein rlf n=1 Tax=Phtheirospermum japonicum TaxID=374723 RepID=A0A830CAD9_9LAMI)

HSP 1 Score: 108 bits (269), Expect = 2.520e-28
Identity = 44/77 (57.14%), Postives = 60/77 (77.92%), Query Frame = 1
Query:    4 RVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLEE 234
            R+IS+ EV+QHKSE D WTV +G+VYN++P++ +HPGG   LMK  G+DCT+LFNKYH WVN E L+   ++G L+E
Sbjct:   23 RLISLNEVKQHKSEGDMWTVLKGRVYNLSPYMKFHPGGVDMLMKAVGKDCTSLFNKYHAWVNAEFLMEKCLVGILDE 99          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: A0A7S2DDF3_9STRA (Hypothetical protein (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2DDF3_9STRA)

HSP 1 Score: 110 bits (274), Expect = 7.320e-28
Identity = 49/86 (56.98%), Postives = 60/86 (69.77%), Query Frame = 1
Query:    1 MRVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLEEEGEKASGD 258
            +R+IS  EV  H SE D WTVFRGKVYN+ P+L YHPGG   LM+ AG+DCT LF+KYH WVN   L+  LV+G L+E+ E    D
Sbjct:   44 LRMISAHEVAMHSSESDMWTVFRGKVYNITPYLDYHPGGVDILMQSAGKDCTVLFDKYHSWVNARGLIGPLVVGELDEDSEPRPAD 129          
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Match: A0A7I8J0D6_SPIIN (Hypothetical protein n=2 Tax=Spirodela intermedia TaxID=51605 RepID=A0A7I8J0D6_SPIIN)

HSP 1 Score: 108 bits (271), Expect = 9.270e-28
Identity = 46/77 (59.74%), Postives = 59/77 (76.62%), Query Frame = 1
Query:    4 RVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRDCTTLFNKYHPWVNGESLLANLVLGFLEE 234
            R+IS+ EV+QHK E   WTV +G+VYN++P++ +HPGG   LMK AGRDCT LFNKYH WVN E LL   ++GFL+E
Sbjct:   94 RLISLDEVKQHKKEGPIWTVLKGRVYNISPYMKFHPGGVDMLMKAAGRDCTALFNKYHAWVNAEFLLEKCLVGFLDE 170          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig10373.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FWC5_ECTSI3.850e-3667.05Similar to Cytochrome b5 reductase 4 n=1 Tax=Ectoc... [more]
A0A812XSF5_SYMMI2.750e-3156.79CYB5R4 protein n=1 Tax=Symbiodinium microadriaticu... [more]
A0A835ZLC4_9STRA1.600e-3056.98Cytochrome b5-like heme/steroid binding domain-con... [more]
UPI000D153F642.830e-3061.84cytochrome b5 domain-containing protein RLF-like i... [more]
A0A2K1IWB2_PHYPA3.970e-2961.84Cytochrome b5 heme-binding domain-containing prote... [more]
D5ADU0_PICSI8.940e-2961.84Cytochrome b5 heme-binding domain-containing prote... [more]
D8SX26_SELML1.990e-2859.74Cytochrome b5 heme-binding domain-containing prote... [more]
A0A830CAD9_9LAMI2.520e-2857.14Cytochrome b5 domain-containing protein rlf n=1 Ta... [more]
A0A7S2DDF3_9STRA7.320e-2856.98Hypothetical protein (Fragment) n=1 Tax=Dictyocha ... [more]
A0A7I8J0D6_SPIIN9.270e-2859.74Hypothetical protein n=2 Tax=Spirodela intermedia ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig10373contigP-wetherbeei_contig10373:472..1641 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7FWC5
Preferred nameCYB5R4
PFAMsCS,Cyt-b5,FAD_binding_6,NAD_binding_1
Model size351
Max annot lvl2759|Eukaryota
KEGG rclassRC02812
KEGG koko:K00326,ko:K10534
KEGG ReactionR00100,R00794,R00796
KEGG Pathwayko00520,ko00910,ko01120,map00520,map00910,map01120
KEGG ModuleM00531
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0002790,GO:0003032,GO:0003674,GO:0003824,GO:0003958,GO:0004128,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006066,GO:0006091,GO:0006139,GO:0006629,GO:0006694,GO:0006696,GO:0006725,GO:0006732,GO:0006733,GO:0006739,GO:0006753,GO:0006778,GO:0006793,GO:0006796,GO:0006801,GO:0006807,GO:0006810,GO:0006811,GO:0006820,GO:0006950,GO:0006974,GO:0007154,GO:0007267,GO:0008104,GO:0008150,GO:0008152,GO:0008202,GO:0008204,GO:0008610,GO:0009058,GO:0009117,GO:0009306,GO:0009593,GO:0009628,GO:0009914,GO:0009987,GO:0010817,GO:0012505,GO:0015031,GO:0015701,GO:0015711,GO:0015833,GO:0016020,GO:0016125,GO:0016126,GO:0016128,GO:0016129,GO:0016174,GO:0016491,GO:0016651,GO:0016653,GO:0019362,GO:0019637,GO:0020037,GO:0023052,GO:0023061,GO:0030072,GO:0030073,GO:0030154,GO:0031984,GO:0032502,GO:0032940,GO:0033013,GO:0033036,GO:0033500,GO:0033554,GO:0034641,GO:0036094,GO:0042168,GO:0042175,GO:0042221,GO:0042440,GO:0042493,GO:0042592,GO:0042593,GO:0042886,GO:0043167,GO:0043168,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044107,GO:0044108,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045184,GO:0046165,GO:0046483,GO:0046496,GO:0046677,GO:0046879,GO:0046903,GO:0046906,GO:0048037,GO:0048468,GO:0048471,GO:0048856,GO:0048869,GO:0048878,GO:0050660,GO:0050662,GO:0050664,GO:0050896,GO:0051179,GO:0051186,GO:0051234,GO:0051606,GO:0051716,GO:0055086,GO:0055114,GO:0065007,GO:0065008,GO:0070482,GO:0071702,GO:0071704,GO:0071705,GO:0072524,GO:0072593,GO:0097159,GO:0097384,GO:0098827,GO:1901265,GO:1901360,GO:1901362,GO:1901363,GO:1901564,GO:1901576,GO:1901615,GO:1901617,GO:1902652,GO:1902653
Exons3
Evalue6.31e-37
EggNOG OGsCOG5274@1|root,KOG0536@2759|Eukaryota
Ec32 ortholog descriptionCytochrome b5-like heme/steroid binding domain
Ec32 orthologEc-25_000490.1
EC1.6.2.2,1.7.1.1,1.7.1.2,1.7.1.3
Descriptionoxidoreductase activity, acting on NAD(P)H, heme protein as acceptor
Cds size351
COG categoryC
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185866.1332552-CDS-P-wetherbeei_contig10373:471..6151683185866.1332552-CDS-P-wetherbeei_contig10373:471..615Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10373 472..615 -
1683185866.1448534-CDS-P-wetherbeei_contig10373:1093..12251683185866.1448534-CDS-P-wetherbeei_contig10373:1093..1225Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10373 1094..1225 -
1683185866.1544588-CDS-P-wetherbeei_contig10373:1566..16411683185866.1544588-CDS-P-wetherbeei_contig10373:1566..1641Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10373 1567..1641 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig10373.1.1prot_P-wetherbeei_contig10373.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig10373 472..1641 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig10373.1.1

>prot_P-wetherbeei_contig10373.1.1 ID=prot_P-wetherbeei_contig10373.1.1|Name=mRNA_P-wetherbeei_contig10373.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=117bp
MRVISMAEVRQHKSEHDCWTVFRGKVYNMAPFLAYHPGGKPELMKGAGRD
CTTLFNKYHPWVNGESLLANLVLGFLEEEGEKASGDAAPAAAAAAAAAAP
TAAGERIAGSAGGAAGG
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mRNA from alignment at P-wetherbeei_contig10373:472..1641-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig10373.1.1 ID=mRNA_P-wetherbeei_contig10373.1.1|Name=mRNA_P-wetherbeei_contig10373.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1170bp|location=Sequence derived from alignment at P-wetherbeei_contig10373:472..1641- (Phaeothamnion wetherbeei SAG_119_79)
ATGCGCGTCATATCCATGGCCGAAGTCAGACAGCACAAGTCTGAGCACGA TTGCTGGACTGTCTTTCGCGGCAAGGTGTGCCTCCTGTGTCTTGTTAGCG AGAAAGTTATTGCTAATATTTTCTTTCTCGCATGTGTTGTACCAGTTCTC CGCTTTTCGTATGAGTAGTACTATCTCCCCCCTTCTGTTCGAGGTTGTCG TGTGTTTAGCACCTTTTCTAGCTGCTGAAACTAGGCCGTTTGAAGAGCGA GCGTCGCCGGATGATCGCTATGAAAGCATCCGGTCAGCCGTAGCGAGGCT GTCCAATCGAATCGCTGTCGATGCCGCATCCAAAAATGCGTTTCGGCCCC CTGTCGTCACACCGACCGTTCCGAAGAACTCTTCCTTGTCTCTCTTTCAT CTACCCTTTCTCGCAGGTATACAACATGGCGCCGTTTCTGGCGTATCACC CGGGCGGCAAGCCGGAGCTGATGAAGGGCGCTGGCCGCGACTGCACCACC CTCTTCAACAAGTACCACCCCTGGGTCAACGGCGAAAGCCTCCTAGCGGT CAGTGTCTCTGCCTTTTGTATTTCTCTCTCTCTCTCTCTCTCTCTTTTCG CTTTCTCTCTGCCTGTATTTTGTTGACGGCGAGGGCCTCAGGTCAGTCCC TCCTCCTCGTTTGTATCTCTTCTCTCTTCTCTCTCCTGTTTCTCTTTCTT CTTCTCTTTTTCGTCCTCCTTTCGTCTGCTGCGAGAGTCGTGAGGGACCA GATATCCGTTTCTCCTCCTGTTTCGTTTCTCTCTTCTCTTCCGTTTCGTT CTTGTCCTTCACTTCTCTCTTTTTCCATCTTGTGCCGTTCTTTCAGCGTT AGCGCGTCCAGCGATTATGGCGCCTCTGACTTTCCAAATCCCATCATAGT ATACTTAAGGATCAGTTCGGCGGTGTGTTTTCTCGCTTCGCCGAAGGTTC TACGCGTTGGTGCATTGTGCAGCGTAGTTTAACCTTATGTATACCCTTCT TCCTTTGCGTTCTCTTCCTCCGGCAGAATTTGGTGCTCGGCTTCCTGGAA GAGGAGGGAGAAAAGGCCTCTGGCGACGCCGCCCCAGCAGCAGCAGCGGC GGCAGCTGCGGCGGCGCCAACGGCGGCGGGAGAGAGAATAGCCGGCTCTG CTGGCGGTGCCGCCGGCGGT
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Coding sequence (CDS) from alignment at P-wetherbeei_contig10373:472..1641-

>mRNA_P-wetherbeei_contig10373.1.1 ID=mRNA_P-wetherbeei_contig10373.1.1|Name=mRNA_P-wetherbeei_contig10373.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=351bp|location=Sequence derived from alignment at P-wetherbeei_contig10373:472..1641- (Phaeothamnion wetherbeei SAG_119_79)
ATGCGCGTCATATCCATGGCCGAAGTCAGACAGCACAAGTCTGAGCACGA
TTGCTGGACTGTCTTTCGCGGCAAGGTATACAACATGGCGCCGTTTCTGG
CGTATCACCCGGGCGGCAAGCCGGAGCTGATGAAGGGCGCTGGCCGCGAC
TGCACCACCCTCTTCAACAAGTACCACCCCTGGGTCAACGGCGAAAGCCT
CCTAGCGAATTTGGTGCTCGGCTTCCTGGAAGAGGAGGGAGAAAAGGCCT
CTGGCGACGCCGCCCCAGCAGCAGCAGCGGCGGCAGCTGCGGCGGCGCCA
ACGGCGGCGGGAGAGAGAATAGCCGGCTCTGCTGGCGGTGCCGCCGGCGG
T
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