mRNA_P-wetherbeei_contig13308.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig13308.1.1
Unique NamemRNA_P-wetherbeei_contig13308.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig13308.1.1 vs. uniprot
Match: D7FKH8_ECTSI (Peptide n-glycanase, putative n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FKH8_ECTSI)

HSP 1 Score: 169 bits (427), Expect = 2.540e-40
Identity = 143/422 (33.89%), Postives = 199/422 (47.16%), Query Frame = 1 
Query:  304 LCQLPTTAAMAAVGAATGDAAARALLVAAPASCPGGTPTGVSYRPGHPALFLGPNASDLASAVSWSTWVRSCGGAAAAG----AEQQFF----PVSWRLLSYHGVHGGVHQDTKPFCDDGSAG-----FGARPAA---GGGAMPVAPSAVVVEVVGWCGAFLNGIQTAHRVGHAKVRAPRHLGEHGTYRQSVLAVANDGGGSGGGSAARVSAVIVRAGHIVDGLTVRTSDGRERTWGGVGGGGAARTFEVPPGHELVGFYGGVGGHMHHVGIVTVVTGGVRQPAASAAAAATVAQLGADALPFA--YGGDAVGEAGAAMLA----------------------------ALPAATAATAFSAALKYSRNILDHPSEAKFQRVRLNNAYFERSLGAAAGSGALMRALGFAVSPDNDGNIFFVF 1431
            LC L +  A     AAT   +A A+  +A  +C          R G P + +GP A DL     W+T ++  G  A AG    AE++      PV W + + H   GG H+DT+ F DDG  G      G  P A    G A P  P+  V E+VGW G  +NG+Q  + V    VR P+ +G+HG YRQS   +  +GG         ++ + V+AG IVD + VRT+ GRE+TWGG GG    RT+ VP G   +GF+GGVGGH+H +G+     GG       AA A+  + L  D +     Y  D V  A A  LA                                    TA     KY+ N+L  P + K  R+RL N +F+R +G  AG G ++RA+GF ++ D  G + +VF
Sbjct:  909 LCPLRSLPAEGDAAAATTTPSAVAVSASARGTC---------RRAGEPTVSMGP-AVDLVDCPGWTTVLQVPGDMAGAGSADDAEKEEVSAVPPVGWVVTTVHPAVGGTHEDTEEF-DDGPVGCPAFVMGGSPVALTADGSAPPFLPAGRVREIVGWSGDAVNGVQVVYDVQGDAVRGPKRMGDHGLYRQSNFVLDVEGGEV-------LTEISVKAGAIVDSVRVRTNKGREKTWGGAGGH-LQRTWHVPTGSSFLGFHGGVGGHVHSLGVTLAERGG------QAAGASGESSLALDPVVKTNLYAADRVARACAQFLAFNAPXXXXXXXXXXXXXXXXXXXXXXXXXXALEEVVTALETMRKYADNLLASPLDPKVSRIRLANGFFDRKIGRLAGGGGVVRAMGFELA-DEGGRMHYVF 1304
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BLAST of mRNA_P-wetherbeei_contig13308.1.1 vs. uniprot
Match: A0A836CB71_9STRA (Putative peptide n-glycanase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CB71_9STRA)

HSP 1 Score: 162 bits (411), Expect = 2.640e-38
Identity = 139/351 (39.60%), Postives = 178/351 (50.71%), Query Frame = 1 
Query:  418 TGVSYRPGHPALFLGPNASDLA-SAVSWSTWV--RSCG--GAAAAGAEQQFFPVS-WRLLSYHGVHGGVHQDTKPFCDDGSAGFGARPAAGGGAMPVA----------PSAVVVEVVGWCG-AFLNGIQTAHRVGHAKVRAPRHLGEHGTYRQSVLAVANDGGGSGGGSAARVSAVIVRAGHIVDGLTVRTSDGRERTWGGVGGGGAARTFEVPPGHELVGFYGGVGGHMHHVGIVTVVTGGVRQPAASAAAAATVAQLGADALPFAYGGDAVGEAGAAML----------AALPAATAATAFSAALKYSRNILDHPSEAKFQRVRLNNAYFERSLGAAAGSGALMRALGF 1389
            T +SY PG P L      S  A  A  WST +   S G  GAAA GA  Q      WR++  H   G  H DT  F D  +  F A  A GG A  V           P   VVE++GW   A +NG+Q  +R+G  +    RHLG+HGTYRQS + + NDG G        V++V VRAG +VD +TV T+ GR  TWG        R++ VP GH  +GF GGVGGH+H +GI+TV        AA+AAA A + +  A A+P  Y  D    A AA++          +   AA A  A   ALKY+ N+L     AKF R+R  NA F   +G+  G   L+RALGF
Sbjct:  860 TALSYCPGQPVLIGEVCGSGGAVHAPGWSTTLLPESVGVDGAAARGALPQGDDRGVWRVVETHDATGSAHDDTIAFSDADTV-FPAVGADGGAAAAVVLGGSIGGALLPLPHVVEILGWASDAHVNGVQLHYRIGTVRATGVRHLGDHGTYRQSAMML-NDGEG--------VASVDVRAGLLVDQVTVTTTKGRSHTWGSALTESPLRSYSVPDGHTFLGFSGGVGGHLHSLGIITVSES--ETLAAAAAADADMPRFAA-AVPLLYSADPARRAVAALMHLSADGGSSDSEAAAAAARIAAETALKYAANVLR--DAAKFSRIRAGNAVFAAKIGSVRGGPLLLRALGF 1195
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BLAST of mRNA_P-wetherbeei_contig13308.1.1 vs. uniprot
Match: A0A7S2XV66_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XV66_9STRA)

HSP 1 Score: 97.4 bits (241), Expect = 3.110e-18
Identity = 97/318 (30.50%), Postives = 145/318 (45.60%), Query Frame = 1 
Query:  559 PVSWRLLSYHGVHGGVHQDTKPFCD----------DGSAGFGARPAAGGGAMPVAPSAVVVEVVGWCGAFLNGIQTAHR-VGHAKVR--APRH-------LGEHGTYRQSVLAVANDGGGSGGGSAARVSAVIVRAGHIVDGLTVRTSDGRERTWGGVGGGGAARTFEVPPGHELVGFYGGVGGHMHHVGIVTVVTGGVRQPAASAAAAATVAQLGADALPFAYGG---------DAVGEAGAAMLAALPAATA---ATAFSAALKYSRNILDHPSEAKFQRVRLNNAYFERSLGAAAGSGALMRALGFAVSPDNDGN 1416
            P  +R +++    G  H+DT+   D          + +   GA  A     +PVA    +  +VGW G+ LNG+Q  +R  G A++    P H       LG  G Y++S   +              +  V VR+G +VD L V TS GR   WGG GG     T+E+P G  L GFYGG GGH+HH+G + +     ++   S A+  +         PFA  G          AV E     +   P AT        +A  KY + I + P++ KF+RV++ N+YF+R++G       LM A+GF ++ D   N
Sbjct:   47 PSPFRSITWAEAAGRTHRDTRVVNDAEVFLPHHRNECTWPLGASAAVVPELLPVAR---IARIVGWAGSLLNGVQLFYRWPGRAEMGDLLPGHKLFGDDGLGLGGEYQKSSFDLVEG---------EEIVKVFVRSGALVDALGVETSQGRRFWWGGQGG--DLTTWEIPQGSFLGGFYGGYGGHVHHLGPIII-----QEHCPSGASLDS---------PFAVQGAYGLKRRVYQAVHE-----ILEAPVATKDQKLAVLAAVSKYLKKIQEQPNDVKFRRVKVGNSYFQRNIGVHMYGCQLMAAVGFFLNFDKTTN 331
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig13308.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
D7FKH8_ECTSI2.540e-4033.89Peptide n-glycanase, putative n=2 Tax=Ectocarpus T... [more]
A0A836CB71_9STRA2.640e-3839.60Putative peptide n-glycanase n=1 Tax=Tribonema min... [more]
A0A7S2XV66_9STRA3.110e-1830.50Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig13308contigP-wetherbeei_contig13308:8..1859 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FKH8
Preferred namePNG1
PFAMsPUL,Peptidase_C97,Rad4,Thioredoxin,Transglut_core
Model size1852
Max annot lvl2759|Eukaryota
KEGG koko:K01456,ko:K03671
KEGG Pathwayko04141,ko04621,ko05418,map04141,map04621,map05418
Hectar predicted targeting categoryother localisation
GOsGO:0000224,GO:0000302,GO:0001101,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005829,GO:0006056,GO:0006058,GO:0006464,GO:0006508,GO:0006511,GO:0006515,GO:0006516,GO:0006517,GO:0006807,GO:0006950,GO:0006979,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009100,GO:0009636,GO:0009719,GO:0009725,GO:0009751,GO:0009987,GO:0010033,GO:0010035,GO:0010188,GO:0010193,GO:0010243,GO:0010498,GO:0014070,GO:0016787,GO:0016810,GO:0016811,GO:0019538,GO:0019941,GO:0030163,GO:0030433,GO:0033554,GO:0034976,GO:0035966,GO:0035967,GO:0035977,GO:0036211,GO:0036503,GO:0042221,GO:0042493,GO:0043161,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043632,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0046677,GO:0046872,GO:0050896,GO:0051603,GO:0051716,GO:0051787,GO:0051788,GO:0070887,GO:0071218,GO:0071310,GO:0071704,GO:0071712,GO:0097466,GO:1901135,GO:1901136,GO:1901564,GO:1901565,GO:1901575,GO:1901698,GO:1901700,GO:1904587
Exons1
Evalue6.42e-43
EggNOG OGsKOG0908@1|root,KOG0909@1|root,KOG0908@2759|Eukaryota,KOG0909@2759|Eukaryota
EC3.5.1.52
DescriptionSpecifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins
Cds size1224
COG categoryT
BRITEko00000,ko00001,ko01000,ko03110
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185980.9321344-UTR-P-wetherbeei_contig13308:7..3051683185980.9321344-UTR-P-wetherbeei_contig13308:7..305Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig13308 8..305 -
1683185980.9527936-UTR-P-wetherbeei_contig13308:1529..18591683185980.9527936-UTR-P-wetherbeei_contig13308:1529..1859Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig13308 1530..1859 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185980.9433186-CDS-P-wetherbeei_contig13308:305..15291683185980.9433186-CDS-P-wetherbeei_contig13308:305..1529Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig13308 306..1529 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig13308.1.1prot_P-wetherbeei_contig13308.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig13308 306..1529 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig13308.1.1

>prot_P-wetherbeei_contig13308.1.1 ID=prot_P-wetherbeei_contig13308.1.1|Name=mRNA_P-wetherbeei_contig13308.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=408bp
MAAVGAATGDAAARALLVAAPASCPGGTPTGVSYRPGHPALFLGPNASDL
ASAVSWSTWVRSCGGAAAAGAEQQFFPVSWRLLSYHGVHGGVHQDTKPFC
DDGSAGFGARPAAGGGAMPVAPSAVVVEVVGWCGAFLNGIQTAHRVGHAK
VRAPRHLGEHGTYRQSVLAVANDGGGSGGGSAARVSAVIVRAGHIVDGLT
VRTSDGRERTWGGVGGGGAARTFEVPPGHELVGFYGGVGGHMHHVGIVTV
VTGGVRQPAASAAAAATVAQLGADALPFAYGGDAVGEAGAAMLAALPAAT
AATAFSAALKYSRNILDHPSEAKFQRVRLNNAYFERSLGAAAGSGALMRA
LGFAVSPDNDGNIFFVFSAPSSTTARAALAAAVAGPLAALVAAAEARVAA
APAAVPV*
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mRNA from alignment at P-wetherbeei_contig13308:8..1859-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig13308.1.1 ID=mRNA_P-wetherbeei_contig13308.1.1|Name=mRNA_P-wetherbeei_contig13308.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1852bp|location=Sequence derived from alignment at P-wetherbeei_contig13308:8..1859- (Phaeothamnion wetherbeei SAG_119_79)
CAGGCGGCGCCGGCGCTGGGGCCAGCCGCCCTGGCGACGCTTCAAGGACT
GCTGCCGCCGGGGGGCGCCCCGCTGGCAGAGCCTGACCCGCGCGGCCCGT
GGACGCTGGTCGGATGGCGGGGCGGCGGCGGTCCCGCCGGCGACAGTGGC
ATCGGCGGCTTTGGCGGCTACGAGCGCCGTGCTCTTGGAGAGGGCGCCGC
ACGGCTAGCGGCTGAAATCGTCTTCAGCAGTGAGACGAGGTCGCCGTCGG
CGCCGACAGCGCCACAGCTCGTGGAGCGGGAGGGCTTCGTCGGAACGGCC
GTCCTCTGCCAACTGCCGACCACAGCCGCCATGGCAGCGGTAGGCGCAGC
CACCGGTGACGCGGCGGCTCGCGCACTCCTCGTAGCAGCGCCAGCGTCGT
GCCCCGGCGGGACGCCGACCGGCGTCAGCTACCGCCCCGGACACCCGGCG
CTGTTCCTGGGGCCCAACGCAAGCGACCTCGCGTCGGCAGTTTCGTGGAG
CACGTGGGTTCGCAGCTGCGGCGGCGCGGCAGCCGCGGGAGCGGAGCAGC
AGTTCTTCCCGGTGAGCTGGCGCCTGCTCTCATACCACGGCGTCCACGGC
GGGGTGCATCAAGACACAAAGCCATTCTGCGACGACGGCTCTGCCGGCTT
TGGCGCCCGGCCGGCGGCGGGAGGGGGTGCGATGCCGGTGGCGCCGTCGG
CAGTAGTGGTTGAGGTGGTGGGTTGGTGCGGCGCCTTTTTGAACGGGATC
CAGACAGCACACCGGGTGGGACACGCTAAGGTCCGCGCCCCTCGGCACCT
CGGCGAGCACGGCACGTACCGCCAGTCCGTGCTGGCGGTTGCCAACGATG
GTGGCGGTAGCGGCGGCGGCAGCGCGGCGCGAGTGTCGGCTGTCATTGTC
CGCGCCGGTCACATCGTGGACGGGCTGACGGTACGGACATCAGACGGACG
GGAGCGGACTTGGGGCGGCGTGGGCGGCGGCGGTGCCGCTCGGACGTTCG
AAGTGCCGCCGGGCCACGAGCTCGTAGGCTTCTACGGTGGCGTTGGTGGT
CACATGCACCACGTCGGGATCGTGACAGTCGTCACGGGCGGCGTTCGTCA
GCCAGCAGCGTCGGCGGCGGCGGCAGCGACGGTTGCACAGCTTGGGGCGG
ATGCGCTGCCGTTTGCCTACGGCGGTGATGCGGTCGGGGAGGCGGGGGCG
GCGATGCTTGCGGCGCTGCCCGCGGCGACGGCGGCGACAGCGTTCTCGGC
GGCGTTAAAGTACAGCCGCAACATCCTTGACCACCCGTCTGAGGCCAAGT
TCCAGCGCGTGCGCCTCAACAACGCATACTTCGAGCGCAGCCTCGGCGCT
GCGGCGGGGTCGGGCGCTCTGATGCGGGCCCTCGGATTTGCAGTTTCGCC
CGACAACGACGGCAATATCTTCTTCGTCTTTTCTGCGCCGTCCTCTACGA
CGGCCCGTGCGGCCCTGGCGGCGGCGGTGGCGGGCCCACTGGCTGCTTTG
GTGGCGGCGGCGGAGGCGCGTGTGGCAGCAGCACCTGCCGCCGTGCCTGT
TTGAAGAGAATGTTGCGAGTCTCTTGGGTGGAAGGGCCTCAGCGTTTCAG
CGCAGCAGGGCGAGGGCGGCAGAACTGCTGCCCATTTCTGCTGACCAAGC
GGGAACAACCAAACCATAGTGTATTGAAATAGGGCTGGCATGCACATCTT
GACTGCTCAAGTAGAGCCCCTTCAGGCGGAGAAATGTGTGCTTCGCGTCC
TGCTCGTGAAGAGCACTCCGTCTTGTAGCGAGGGGGAGGAGTAGCGGAGG
GGGGGCTCTGCTCTAATGTTGCCCTGATAATGATGATAAGTAAATTGGGG
AC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig13308:8..1859-

>mRNA_P-wetherbeei_contig13308.1.1 ID=mRNA_P-wetherbeei_contig13308.1.1|Name=mRNA_P-wetherbeei_contig13308.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=1224bp|location=Sequence derived from alignment at P-wetherbeei_contig13308:8..1859- (Phaeothamnion wetherbeei SAG_119_79)
ATGGCAGCGGTAGGCGCAGCCACCGGTGACGCGGCGGCTCGCGCACTCCT
CGTAGCAGCGCCAGCGTCGTGCCCCGGCGGGACGCCGACCGGCGTCAGCT
ACCGCCCCGGACACCCGGCGCTGTTCCTGGGGCCCAACGCAAGCGACCTC
GCGTCGGCAGTTTCGTGGAGCACGTGGGTTCGCAGCTGCGGCGGCGCGGC
AGCCGCGGGAGCGGAGCAGCAGTTCTTCCCGGTGAGCTGGCGCCTGCTCT
CATACCACGGCGTCCACGGCGGGGTGCATCAAGACACAAAGCCATTCTGC
GACGACGGCTCTGCCGGCTTTGGCGCCCGGCCGGCGGCGGGAGGGGGTGC
GATGCCGGTGGCGCCGTCGGCAGTAGTGGTTGAGGTGGTGGGTTGGTGCG
GCGCCTTTTTGAACGGGATCCAGACAGCACACCGGGTGGGACACGCTAAG
GTCCGCGCCCCTCGGCACCTCGGCGAGCACGGCACGTACCGCCAGTCCGT
GCTGGCGGTTGCCAACGATGGTGGCGGTAGCGGCGGCGGCAGCGCGGCGC
GAGTGTCGGCTGTCATTGTCCGCGCCGGTCACATCGTGGACGGGCTGACG
GTACGGACATCAGACGGACGGGAGCGGACTTGGGGCGGCGTGGGCGGCGG
CGGTGCCGCTCGGACGTTCGAAGTGCCGCCGGGCCACGAGCTCGTAGGCT
TCTACGGTGGCGTTGGTGGTCACATGCACCACGTCGGGATCGTGACAGTC
GTCACGGGCGGCGTTCGTCAGCCAGCAGCGTCGGCGGCGGCGGCAGCGAC
GGTTGCACAGCTTGGGGCGGATGCGCTGCCGTTTGCCTACGGCGGTGATG
CGGTCGGGGAGGCGGGGGCGGCGATGCTTGCGGCGCTGCCCGCGGCGACG
GCGGCGACAGCGTTCTCGGCGGCGTTAAAGTACAGCCGCAACATCCTTGA
CCACCCGTCTGAGGCCAAGTTCCAGCGCGTGCGCCTCAACAACGCATACT
TCGAGCGCAGCCTCGGCGCTGCGGCGGGGTCGGGCGCTCTGATGCGGGCC
CTCGGATTTGCAGTTTCGCCCGACAACGACGGCAATATCTTCTTCGTCTT
TTCTGCGCCGTCCTCTACGACGGCCCGTGCGGCCCTGGCGGCGGCGGTGG
CGGGCCCACTGGCTGCTTTGGTGGCGGCGGCGGAGGCGCGTGTGGCAGCA
GCACCTGCCGCCGTGCCTGTTTGA
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