mRNA_P-wetherbeei_contig54795.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig54795.1.1
Unique NamemRNA_P-wetherbeei_contig54795.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: UPI00199EBA03 (Hydrogenase 4 subunit B n=1 Tax=Hyphomicrobiales bacterium TaxID=1909294 RepID=UPI00199EBA03)

HSP 1 Score: 154 bits (389), Expect = 3.460e-44
Identity = 76/118 (64.41%), Postives = 93/118 (78.81%), Query Frame = 1 
Query:   16 LPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            LPLG+   GL  ++DALSAFFG++VNL V  T++YG GLDR  D+S R+EP+FP + +AMN VLIA DA  FLFSWELMSLTSW+LVV+RHRD   R A +VYL+MA IGT +LLFAF
Sbjct:   20 LPLGLPVIGLHIKLDALSAFFGLIVNLGVLTTAIYGMGLDRDRDLSARVEPFFPAYALAMNLVLIAGDAFGFLFSWELMSLTSWVLVVSRHRDAENRHAGHVYLVMAAIGTASLLFAF 137
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A1F3A0F5_9PROT (Hydrogenase 4 subunit B n=2 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A1F3A0F5_9PROT)

HSP 1 Score: 150 bits (379), Expect = 5.920e-40
Identity = 77/123 (62.60%), Postives = 90/123 (73.17%), Query Frame = 1 
Query:    1 EVKATLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            E+   LP+G+   GL  R+DALSAFFGIV+N  V   S+YG G DR  +++PR+EP FPLF   MN VLIADDA AFLFSWELMSL SW LVVARH D   R A Y+YL+MA IGT ALLF+F
Sbjct:   56 ELTVWLPIGLPDIGLHLRLDALSAFFGIVINGGVLAASVYGMGFDRAKELTPRVEPLFPLFAATMNLVLIADDAYAFLFSWELMSLASWALVVARHTDPESRRAGYLYLIMAAIGTSALLFSF 178
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A529XR89_9HYPH (Hydrogenase 4 subunit B (Fragment) n=2 Tax=Mesorhizobium sp. TaxID=1871066 RepID=A0A529XR89_9HYPH)

HSP 1 Score: 135 bits (341), Expect = 1.150e-37
Identity = 70/123 (56.91%), Postives = 85/123 (69.11%), Query Frame = 1 
Query:    1 EVKATLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            E  ATLPLG+  TG RFR+D LSAFF +VVNL   +TSLYG G  R      R+ P++P F+  MN V++A+DA +FL SWE MSL SW LV+A HRD   R A Y+YL+MA  GTMALL AF
Sbjct:   26 EASATLPLGLPWTGARFRLDGLSAFFLVVVNLGGTITSLYGLGYGRHESQPHRVLPFYPAFLAGMNLVVLANDAFSFLLSWEFMSLASWALVMAHHRDEANRRAGYIYLVMASFGTMALLLAF 148
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A1T4PYD1_9HYPH (Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit n=1 Tax=Enhydrobacter aerosaccus TaxID=225324 RepID=A0A1T4PYD1_9HYPH)

HSP 1 Score: 143 bits (360), Expect = 2.620e-37
Identity = 76/125 (60.80%), Postives = 92/125 (73.60%), Query Frame = 1 
Query:    1 EVKATLPLGIAPT--GLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            ++ A LP+G+ PT  G   R+D LSAFFG+V+N  V   SLYG GLDR  D++PR+EP FPLF  AMN VL+ADDA AFL SWELMSL SW LVVARH  +  R A ++YL+MA IGT AL+FAF
Sbjct:   53 QLAARLPIGL-PTIGGFHLRLDPLSAFFGVVINGGVLAASLYGMGLDRAKDLTPRVEPLFPLFAAAMNLVLLADDAYAFLLSWELMSLASWALVVARHTSVENRRAGHLYLVMAAIGTTALIFAF 176
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A1H8TDM4_9PROT (Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit n=3 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A1H8TDM4_9PROT)

HSP 1 Score: 142 bits (359), Expect = 3.650e-37
Identity = 73/123 (59.35%), Postives = 88/123 (71.54%), Query Frame = 1 
Query:    1 EVKATLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            E+   LP+G+   G   R+D LSAFFGIV+N  +   S+YG G DR  +++ R+EP FPLF  AMN VL+ADDA AFLFSWELMSL SW LVV RH D   R AA++YL+MA IGT ALLFAF
Sbjct:   56 ELTQQLPIGLPTIGFHLRLDPLSAFFGIVINGGILAASVYGMGFDRAKELTRRVEPLFPLFAAAMNLVLLADDAYAFLFSWELMSLASWALVVVRHTDPESRRAAHLYLVMAAIGTAALLFAF 178
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: E3I709_RHOVT (NADH/Ubiquinone/plastoquinone (Complex I) n=5 Tax=Rhodomicrobium TaxID=1068 RepID=E3I709_RHOVT)

HSP 1 Score: 140 bits (353), Expect = 2.530e-36
Identity = 75/122 (61.48%), Postives = 89/122 (72.95%), Query Frame = 1 
Query:    4 VKATLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            +K  LP+G+   GL  R+D LSAFFG +VN+ V + S+YG G+ R  D+S RIEP +P F  AMNAVL+ADDA  FLF WELMSL+SW LVV+RH D   R A YVYLLMA  GTMALLFAF
Sbjct:   61 LKEHLPIGLPGVGLHIRLDGLSAFFGFIVNVGVLMASIYGAGIKRD-DLSRRIEPLYPAFCAAMNAVLLADDAFGFLFFWELMSLSSWALVVSRHDDPDNRKAGYVYLLMAAGGTMALLFAF 181
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A530A3W5_9HYPH (Hydrogenase 4 subunit B (Fragment) n=1 Tax=Mesorhizobium sp. TaxID=1871066 RepID=A0A530A3W5_9HYPH)

HSP 1 Score: 130 bits (326), Expect = 1.360e-35
Identity = 66/119 (55.46%), Postives = 84/119 (70.59%), Query Frame = 1 
Query:   13 TLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            TLP+G+  TG RFR+D LSAFF +VVNL  A+TSLY  G  R   +  R+ P++P F+  MN V++A+DA +FL SWE MSL SW LV+A HRD   R A Y+YL+MA  GT+ALL AF
Sbjct:   65 TLPIGLPWTGARFRLDGLSAFFLVVVNLGGAITSLYALGYGRHESLRHRVLPFYPAFLAGMNLVVLANDAFSFLLSWEFMSLASWALVMAHHRDEANRRAGYIYLVMASFGTLALLLAF 183
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A529XLR7_9HYPH (Hydrogenase 4 subunit B (Fragment) n=1 Tax=Mesorhizobium sp. TaxID=1871066 RepID=A0A529XLR7_9HYPH)

HSP 1 Score: 130 bits (326), Expect = 1.740e-34
Identity = 66/119 (55.46%), Postives = 84/119 (70.59%), Query Frame = 1 
Query:   13 TLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            TLP+G+  TG RFR+D LSAFF +VVNL  A+TSLY  G  R   +  R+ P++P F+  MN V++A+DA +FL SWE MSL SW LV+A HRD   R A Y+YL+MA  GT+ALL AF
Sbjct:   65 TLPIGLPWTGARFRLDGLSAFFLVVVNLGGAITSLYALGYGRHESLRHRVLPFYPAFLAGMNLVVLANDAFSFLLSWEFMSLASWALVMAHHRDEANRRAGYIYLVMASFGTLALLLAF 183
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A090GJV5_MESPL (Hydrogenase 4, membrane subunit n=106 Tax=Bacteria TaxID=2 RepID=A0A090GJV5_MESPL)

HSP 1 Score: 135 bits (339), Expect = 2.180e-34
Identity = 70/123 (56.91%), Postives = 85/123 (69.11%), Query Frame = 1 
Query:    1 EVKATLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            E  ATLPLG+  TG RFR+D LSAFF +VVNL  A+ SLYG G  R      R+ P++P F+  MN V++A+DA +FL SWE MSL SW LV+A HRD   R A Y+YL+MA  GTMALL AF
Sbjct:   60 EASATLPLGLPWTGARFRLDGLSAFFLLVVNLGGAIASLYGLGYGRHESAPHRVMPFYPAFLAGMNLVVLANDAFSFLLSWEFMSLASWALVMAHHRDEANRRAGYIYLVMASFGTMALLLAF 182
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BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Match: A0A3M1JQP0_9PROT (Hydrogenase 4 subunit B (Fragment) n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A3M1JQP0_9PROT)

HSP 1 Score: 127 bits (319), Expect = 2.870e-32
Identity = 68/119 (57.14%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:   13 TLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADMSPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLTVRFAAYVYLLMAVIGTMALLFAF 369
            TLPLG+   G  FR+DAL+AFF +VVNL  AV+SLYG G  +  +   R+ P+FP FV  MN V++ADDA  FL SWE MSL+SW LV+A HRD     A YVYL+MA  GT+ALL AF
Sbjct:   63 TLPLGLPWLGAHFRVDALAAFFLVVVNLGGAVSSLYGLGYGQHEEAPHRVLPFFPAFVGGMNLVVLADDAFVFLLSWEFMSLSSWALVLAHHRDRENVRAGYVYLMMASFGTLALLLAF 181
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig54795.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00199EBA033.460e-4464.41Hydrogenase 4 subunit B n=1 Tax=Hyphomicrobiales b... [more]
A0A1F3A0F5_9PROT5.920e-4062.60Hydrogenase 4 subunit B n=2 Tax=Alphaproteobacteri... [more]
A0A529XR89_9HYPH1.150e-3756.91Hydrogenase 4 subunit B (Fragment) n=2 Tax=Mesorhi... [more]
A0A1T4PYD1_9HYPH2.620e-3760.80Formate hydrogenlyase subunit 3/Multisubunit Na+/H... [more]
A0A1H8TDM4_9PROT3.650e-3759.35Formate hydrogenlyase subunit 3/Multisubunit Na+/H... [more]
E3I709_RHOVT2.530e-3661.48NADH/Ubiquinone/plastoquinone (Complex I) n=5 Tax=... [more]
A0A530A3W5_9HYPH1.360e-3555.46Hydrogenase 4 subunit B (Fragment) n=1 Tax=Mesorhi... [more]
A0A529XLR7_9HYPH1.740e-3455.46Hydrogenase 4 subunit B (Fragment) n=1 Tax=Mesorhi... [more]
A0A090GJV5_MESPL2.180e-3456.91Hydrogenase 4, membrane subunit n=106 Tax=Bacteria... [more]
A0A3M1JQP0_9PROT2.870e-3257.14Hydrogenase 4 subunit B (Fragment) n=1 Tax=Alphapr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig54795contigP-wetherbeei_contig54795:6..386 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog648757.Rvan_0285
Preferred namehyfB
PFAMsProton_antipo_M
Model size381
Max annot lvl28211|Alphaproteobacteria
Hectar predicted targeting categorysignal anchor
Exons1
Evalue4.15e-37
EggNOG OGsCOG0651@1|root,COG0651@2|Bacteria,1MXRW@1224|Proteobacteria,2TZIX@28211|Alphaproteobacteria,3N87S@45401|Hyphomicrobiaceae
DescriptionProton-conducting membrane transporter
Cds size381
COG categoryCP
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186817.7701607-CDS-P-wetherbeei_contig54795:5..3861683186817.7701607-CDS-P-wetherbeei_contig54795:5..386Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig54795 6..386 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig54795.1.1prot_P-wetherbeei_contig54795.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig54795 6..386 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig54795.1.1

>prot_P-wetherbeei_contig54795.1.1 ID=prot_P-wetherbeei_contig54795.1.1|Name=mRNA_P-wetherbeei_contig54795.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=127bp
EVKATLPLGIAPTGLRFRIDALSAFFGIVVNLVVAVTSLYGCGLDRQADM
SPRIEPYFPLFVIAMNAVLIADDALAFLFSWELMSLTSWLLVVARHRDLT
VRFAAYVYLLMAVIGTMALLFAFAAMS
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mRNA from alignment at P-wetherbeei_contig54795:6..386-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig54795.1.1 ID=mRNA_P-wetherbeei_contig54795.1.1|Name=mRNA_P-wetherbeei_contig54795.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=381bp|location=Sequence derived from alignment at P-wetherbeei_contig54795:6..386- (Phaeothamnion wetherbeei SAG_119_79)
GAAGTAAAGGCTACTCTCCCGCTCGGCATTGCCCCAACCGGCCTGCGCTT
CCGCATCGACGCCTTGAGCGCGTTTTTCGGAATTGTCGTGAACTTGGTCG
TCGCCGTCACGAGCCTCTATGGTTGCGGCCTCGACCGCCAGGCCGACATG
TCGCCGCGCATCGAGCCCTATTTTCCCCTCTTCGTGATCGCGATGAACGC
GGTCCTGATCGCAGACGACGCTCTCGCATTCCTCTTCTCTTGGGAGTTGA
TGTCGCTGACCTCGTGGCTTCTCGTCGTCGCGCGCCATCGCGACCTGACA
GTCCGTTTCGCAGCATATGTCTACCTTCTGATGGCCGTGATCGGCACGAT
GGCGTTGCTTTTTGCATTTGCAGCAATGTCC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig54795:6..386-

>mRNA_P-wetherbeei_contig54795.1.1 ID=mRNA_P-wetherbeei_contig54795.1.1|Name=mRNA_P-wetherbeei_contig54795.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=381bp|location=Sequence derived from alignment at P-wetherbeei_contig54795:6..386- (Phaeothamnion wetherbeei SAG_119_79)
GAAGTAAAGGCTACTCTCCCGCTCGGCATTGCCCCAACCGGCCTGCGCTT
CCGCATCGACGCCTTGAGCGCGTTTTTCGGAATTGTCGTGAACTTGGTCG
TCGCCGTCACGAGCCTCTATGGTTGCGGCCTCGACCGCCAGGCCGACATG
TCGCCGCGCATCGAGCCCTATTTTCCCCTCTTCGTGATCGCGATGAACGC
GGTCCTGATCGCAGACGACGCTCTCGCATTCCTCTTCTCTTGGGAGTTGA
TGTCGCTGACCTCGTGGCTTCTCGTCGTCGCGCGCCATCGCGACCTGACA
GTCCGTTTCGCAGCATATGTCTACCTTCTGATGGCCGTGATCGGCACGAT
GGCGTTGCTTTTTGCATTTGCAGCAATGTCC
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