mRNA_P-wetherbeei_contig1240.4.2 (mRNA) Phaeothamnion wetherbeei SAG_119_79

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: D7FYH7_ECTSI (Lipase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FYH7_ECTSI)

HSP 1 Score: 427 bits (1099), Expect = 1.010e-139
Identity = 231/430 (53.72%), Postives = 282/430 (65.58%), Query Frame = 2

Query:  215 LKPTDVEGRRSARELVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLCGGRDHSLALILAANGYDVFVGNNRGNRYSHKNLSKAP-HEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFEDAPPGQYIPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPRCVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLRDL*ETAGRGG 1501
            ++   +EGRRSAREL E+ GF  E+H   T DGFVLVLHR++ P  L                                          DG    GG R  +K      N K+ RP +LLMHGLMQDSEALLCGGRDH+L L LAA G+DVF+GN RGNRYSHK+L ++P   + YWDFSIDELAR D P M+ HVL  +G  +  YIGFSQG+AQAFAA SS+  LQ+KV L VALSPA RA  L+KSLL SLV ANLRF+ LLFGRRR+LP  L WQR+MSR+ FV  +D A+ YLF+W ++ VS +RK ++YPHIYS+ SVKCV HWFQM+ S RL+MF D P  QY+PV YD+ ++  PVA+ YGGRD +VEV SL + LP  VL+  E  +EHLDTMW D A  T+FPKV  L+R +  T G GG
Sbjct:    1 MRSAGIEGRRSARELAEAVGFSLEDHHAVTADGFVLVLHRLVTPDRL------------------------------------------DGTDGDGGGRCEAK------NIKRPRPAVLLMHGLMQDSEALLCGGRDHALGLRLAAAGFDVFLGNARGNRYSHKHLRRSPVTSDAYWDFSIDELARYDTPTMIDHVLKVSGQTQLCYIGFSQGTAQAFAAFSSDPDLQAKVFLFVALSPAVRANHLSKSLLLSLVQANLRFIYLLFGRRRMLPVALTWQRMMSREFFVATIDGAISYLFSWSASEVSAKRKLEVYPHIYSFISVKCVLHWFQMMYSGRLSMFADGPASQYVPVTYDVARVSCPVAIVYGGRDHLVEVESLIEILPNVVLSIREDKYEHLDTMWADTAKDTVFPKVETLVRQMCPTTGNGG 382          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: A0A836CJG0_9STRA (Lipase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CJG0_9STRA)

HSP 1 Score: 426 bits (1095), Expect = 1.180e-139
Identity = 227/407 (55.77%), Postives = 275/407 (67.57%), Query Frame = 2

Query:  257 LVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLCGGRDHSLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFEDAPPGQYIPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPRCVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLRDL 1477
            +VESEGF  E H   TEDGF+LVLHR++    L  + T                                                         NG    PP+L+MHGLMQDSEALL GGR+H+L L LA+ GYDVF+GNNRGNRYSHK+LS +PH+E YW+FSIDELAR DVPA+V  VL  T A +  Y+GFSQGSAQ FAALS++ ALQ++VAL  ALSPAARA+GL+KS+L++LV  NLRF+N+LFGRRR+LP T+MWQ I+SR  FVRA+D AM YLF+W ++ VS  RK QLYPHIYS++SVKCV HWFQMIRSRRLAMF+D     Y PV+YDIT I+ PVA+ +GGRD IVE  SL   LP CVL++CE  +EHLDTMW D A   IFP++ ALLR L
Sbjct:    1 MVESEGFKLEEHQTRTEDGFILVLHRIVP---LKPRET---------------------------------------------------------NGDDAAPPVLVMHGLMQDSEALLAGGREHALGLWLASRGYDVFLGNNRGNRYSHKHLSWSPHDEAYWNFSIDELARYDVPALVGAVLGVTRAPRLAYVGFSQGSAQMFAALSADTALQARVALFAALSPAARAKGLSKSVLTTLVQTNLRFINVLFGRRRMLPMTVMWQNILSRTQFVRAIDCAMWYLFSWSNSQVSHARKCQLYPHIYSFASVKCVVHWFQMIRSRRLAMFDDTQDPAYAPVSYDITLIRCPVAVMFGGRDLIVECESLSAELPNCVLSHCEDKYEHLDTMWADTAKDAIFPRLHALLRAL 347          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: A0A4D9CWT9_9STRA (AB hydrolase-1 domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CWT9_9STRA)

HSP 1 Score: 387 bits (993), Expect = 5.860e-122
Identity = 227/530 (42.83%), Postives = 309/530 (58.30%), Query Frame = 2

Query:   53 VLDWLHLAGALLVLIALQLVQFIIVWSYHLFPFPGLLDAVYNRSRQLQGWFHLHL-----------KPTDVEGRRSARELVESEGFYFENHFLTTEDGFVLVLHRVI----------APSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARM-------GSKMASGPKNGKKV----------------------------RPPILLMHGLMQDSEALLCGGRDHSLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFEDAPPGQYIPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPRCVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLRD 1474
            ++DW HL   +  L A + V F IV  Y  FPFP LLD  Y  S+ LQ WF   L            P     R  A  LVE   F  E H   T DGFVLVLHR++          AP   T+ ST+   +           D G   G     VS    G ++G  +GGG++         S  + GP +                                RPP+L++H LMQDSE+ LCGG +HSLAL+LA  G+DV++GNNRGNRYSHK++S +P ++ YW+FSIDELAR DVPAMV +VL  TG EK  Y+GFSQGSAQAFAA +++ A+  KV+L +AL+   RA+GL+ S LS+LV +N RF+ L+FGR  +L +TLMW +IMS   FV  VD+A  +LF W ++ VS  RK  LY HI+SY+SVK V  WFQ+IR RRLA + D      +PV YD+ ++++PVA+FYGG+DT++++++L   LP C+  + EP++EHLD +W D A   +FP+++ LL +
Sbjct:    7 LVDWAHLLYLVCFLTAAKAVGFFIVTVYAYFPFPALLDRFYLASQGLQSWFSYQLGGAAPGSAGKASPNGNRERLLAESLVEGTPFILETHTALTRDGFVLVLHRLVPKTSGQDGPGAPGQATSSSTSETYVE----------DRGPQRGRSWRKVS----GESNGRKSGGGSQSLQQHIPTSSFFSKGPGSAAAPAVAPALPSAAVPTVTGTESNAVTPLTPGRPPLLMVHALMQDSESFLCGGEEHSLALVLAKAGFDVWLGNNRGNRYSHKHVSLSPSQDAYWNFSIDELARFDVPAMVDYVLRATGQEKLAYVGFSQGSAQAFAAFTTDLAMAKKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSAARKVGLYQHIFSYASVKTVVQWFQLIRLRRLAFYSDDSNRNRVPVEYDLKRLRVPVAVFYGGKDTVIDISALRDVLPACIRWHMEPEYEHLDMIWADTAKDNVFPELVKLLHE 522          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: D3BFI4_POLPP (Carboxylic ester hydrolase n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3BFI4_POLPP)

HSP 1 Score: 277 bits (708), Expect = 7.110e-81
Identity = 174/493 (35.29%), Postives = 265/493 (53.75%), Query Frame = 2

Query:   62 WLHLAGALLVLIALQLVQFIIVWSYHLFPFPGLLDAVYNRSRQLQGWFHLHLKPTDVEGRRSARELVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLCGGR-DHSLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFEDA-----------PPGQY----IPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPR--CVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLRDL*ET 1486
            W  L   L++L+A ++++ II     + PF   L+++    +    +        D+E  R A E++ S G+  E HF+TT DGFVL LHR+  P   T+   +  T            D+G                                ++ G       +P +L+MHG MQ SEA LC    ++SL  ILA  G+DV++GNNRGN+YS K+ +  P +E++W++S+DEL R D+P+MV+ +   T  +   YIGFSQG+AQ +AALS+N  L +K+ L VAL+P +  +G +  ++ SL  +   F+ LLFG++ +LP+T+ W+ ++ +  FV  +D ++ +LF W +  +  + K  LY HI+SY+SVK V HWFQ+I++ R  MF+D             P +Y    IP AY   QI    ALFYGG DT+    +L   LPR   V+ + E  +EHLD MWG  A K IF K++ LL+   +T
Sbjct:   14 WSQLYLTLMILLAERVIRSIISVVLSVIPFSSYLESLATLQKSSDSYTEYE---KDLE--RDAIEMITSRGYPVEEHFVTTPDGFVLGLHRITGPRAFTSPLDSPPTSPR---------DLG--------------------------------VSGGELFAPTGKPAVLIMHGFMQTSEAWLCRSNPNNSLPFILADAGFDVWLGNNRGNKYSFKHTTYTPDQEKFWNWSLDELVRYDLPSMVNFITNRTKLQSISYIGFSQGTAQGWAALSTNTDLAAKINLFVALAPVSTVKGFSNPMIDSLARSRPDFIFLLFGKKSMLPSTIFWRNLLPKQFFVSMIDASVRFLFGWSTINLGEDEKAMLYSHIFSYTSVKSVVHWFQIIQTNRFQMFDDMLLGITPNESSNQPHRYHGRVIP-AYHPGQILTKCALFYGGADTLPNTKALLSHLPRDKVVMVHEEESYEHLDFMWGKDANKKIFSKIVHLLKSTNKT 459          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: F4QD21_CAVFA (Carboxylic ester hydrolase n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4QD21_CAVFA)

HSP 1 Score: 280 bits (716), Expect = 1.140e-80
Identity = 178/495 (35.96%), Postives = 273/495 (55.15%), Query Frame = 2

Query:   41 LFPAVLDWLHLAGALLVLIALQLVQFIIVWSYHLFPFPGLLDAVYNRSRQLQGWFHLHLKPTDVEGRRSARELVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLCGGR-DHSLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFED-----APP----------GQYIPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPR--CVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLR 1471
            LF  +  W  L   LL+L+  ++ + +I   + + PF   ++ V + +R      H++ +  + +  R A EL+ S G+  E H++TT DGF+L LHR+  P      S   +  A+   NG                            PNG   R+G             +P +L+MHG MQ SEA LC    + SL  ILA  G+DV++GNNRGN+YS K+ +  P +ER+W++S+DEL R D+P++V   +  T      YIGFSQG+AQ +AALS+N AL +K+ L VAL+P +  +G +  ++ SL  +   F+ LLFG++ +LP+T+ W++++ ++ FV+ +D ++ +LF W++  +  E K+ LY HI+SY+SVK V HWFQ+I++ R  MF+D      P           G+ IP  Y   QI    ALFYGG+DT+    +L   LP+   VL + E  +EHLD MWG  A K +F K++ LLR
Sbjct:  129 LFRTLWRWSQLYLTLLILLCEKVARGVISIVFSVIPFSSYIECVASVNRYSSK--HIYTE-AEKDLDRDAIELITSRGYPCEEHYVTTPDGFILGLHRITGPR---INSPPQSPPASPRDNG----------------------------PNGADLRVG-------------KPAVLIMHGFMQTSEAWLCRANPEDSLPFILADAGFDVWLGNNRGNKYSFKHTTYTPSQERFWNWSLDELVRYDLPSIVD--VYRTRLPTISYIGFSQGTAQGWAALSTNTALAAKINLFVALAPVSTVKGFSNPMIDSLARSRPDFIFLLFGKKAMLPSTVWWRQVLPKEFFVQLIDSSVRFLFGWKTHNLDEEEKKMLYSHIFSYTSVKSVVHWFQIIQTNRFQMFDDELLGITPNENSNKKHRYHGRVIP-GYHPAQILTKCALFYGGQDTLPNTKALLAHLPKDKVVLIHEEESYEHLDFMWGKTAGKAVFSKIVTLLR 573          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: A0A8J4UW11_9MYCE (Abhydro_lipase domain-containing protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4UW11_9MYCE)

HSP 1 Score: 275 bits (704), Expect = 1.920e-80
Identity = 175/488 (35.86%), Postives = 247/488 (50.61%), Query Frame = 2

Query:   62 WLHLAGALLVLIALQLVQFIIVWSYHLFPFPGLLDAVYNRSRQLQGWFHLHLKPTDVEGRRSARELVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLCGGRDH-SLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFEDA-----------PPGQY----IPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPR--CVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLR 1471
            W  L   LL+LI  ++V+F +   +   PF       Y     L G         D +  R A EL+ S G+  E HF TT DGF+L LHR+  P        +  T      NGG                                             G   +P +L+MHG MQ SEA LC      SL  ILA  G+DV++GNNRGN+YS K+    P +E +W++S+DEL R D P++V+ +    G E   YIGFSQG+AQ +A+LS+N  L  K+ L VAL+P +  +G +  ++ SL  +   F+ LLFG++ +LP+T+ W+  + ++ FVR +D ++  LF W +  +S E K+ LY HI+SY+SVK V HWFQ+I++ R  MF+D             P +Y    IP  Y   QI    ALFYGG D +    +L   LPR   V+ + E  +EHLD MWG  A K +F KV+ LL+
Sbjct:   15 WSQLYLTLLILITERIVRFFLTTFFGFIPF-----TYYFEQLGLTGSGE-EFSEVDKDLERDAIELITSRGYPVEEHFATTPDGFILGLHRITGPKLFDTPPESPPTSPRHPKNGG---------------------------------------------GSSGKPVVLIMHGFMQTSEAWLCRSNPQDSLPFILADAGFDVWLGNNRGNKYSFKHTQYTPKQEEFWNWSLDELVRYDFPSIVNFITERAGVESISYIGFSQGTAQGWASLSTNTTLAKKINLFVALAPVSTVKGFSNPMIDSLAKSRPDFIFLLFGKKSMLPSTIFWRNFLPKELFVRLIDTSVSLLFGWHTNNISQEEKKMLYSHIFSYTSVKSVVHWFQIIQTNRFQMFDDILLGITPHENSKKPHRYHGRVIP-GYHPAQILTKCALFYGGSDNLPNTKALLDHLPRDKVVMVHEEESYEHLDFMWGKDANKKVFSKVVKLLK 450          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: UPI000644ED0E (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644ED0E)

HSP 1 Score: 275 bits (704), Expect = 3.460e-80
Identity = 173/487 (35.52%), Postives = 260/487 (53.39%), Query Frame = 2

Query:   62 WLHLAGALLVLIALQLVQFIIVWSYHLFPFPGLLDAVYNRSRQLQGWFHLHLKPTDVEGRRSARELVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLCGGRDH-SLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFED-----APP----------GQYIPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPR--CVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALL 1468
            W  L  ALL+++   +++F +       PF   ++      + L+G        +  +  R A EL+ S G+  E HF+TT+DGFVL LHR+  P   T+   +A       G  G+   +                              G+  +S   N   V+P +LLMHG M  SE  L     + SL  ILA  GYDV++GNNRGN+YS K+ +  P +E++W++SIDELA+ D+P+MV  +   TG +   YI FSQG+AQ +A LSSNAAL  KV L +AL+P +   G +  ++ SL  +   F+ LLFG++ +LP+ + W+ I+ RD +V+ +D ++ +LF W +  +S E K  LY HI+SY+SVK V HWFQ+I++ R  MF+D      P           G+ IP  Y   QI+   A+FYGG DT+     L + LP+   V+ +  P++EHLD +W   A   +F K++ LL
Sbjct:   11 WSQLTLALLIIVLESVIRFGMSVFTSFIPFSSHIEL----PKSLKGHSPASYSESQKDLERDAIELITSRGYPVEEHFVTTKDGFVLGLHRITGPRAFTSPLNSAPASPRDLGIRGSHASL-----------------------------FGN--SSNNSNSAMVKPAVLLMHGFMMSSEVWLTRTNPNDSLPFILADAGYDVWLGNNRGNKYSFKHTTYTPDQEQFWNWSIDELAKFDMPSMVDFITDRTGHKTITYIAFSQGTAQGWAGLSSNAALARKVDLFIALAPVSTCLGFSNPMVDSLARSRPDFIFLLFGKKSILPSIIFWRNILPRDIYVKLIDGSVSFLFNWTTACLSKEEKPMLYSHIFSYTSVKTVVHWFQIIQTNRFQMFDDILLGVTPTESSDQPHRYHGRVIP-GYHPGQIQTKFAMFYGGSDTLPNTKLLLEHLPKDKSVMVHEVPEYEHLDLIWAKDAHHKVFSKIVKLL 461          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: A0A151Z637_9MYCE (Carboxylic ester hydrolase n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151Z637_9MYCE)

HSP 1 Score: 269 bits (688), Expect = 6.310e-78
Identity = 166/438 (37.90%), Postives = 243/438 (55.48%), Query Frame = 2

Query:  224 TDVEG--RRSARELVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGV-PNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLCGGR-DHSLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFED-----APP---------GQYIPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPR--CVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLRDL 1477
            TD E    R A EL+ S G+  E HF+TT DGF+L LHR+  P        +  T            D+  GNG ++    +    SN G  P+                 +  RP +L+MHG MQ SEA LC    + SL  ILA  G+DV++GNNRGN+YS K+   AP++E +W++S+DEL R D P++V  +   T A    YIGFSQG+AQ +A LS+N  +  K+ L +AL+P +  +G +  ++ SL  +   F+ LLFG++ +LP+T+ W+ I+ ++ FV  +DQ++ +LF W +  ++ E K  LY HI+SY+SVK V HWFQ+I++ R  MF+D      P          G+ IP  Y  +Q+    ALFYGG D +    +L   +PR   V+ + E  +EHLD MWG  A K IF K++ L+  L
Sbjct:   46 TDAEKDYERDAIELITSRGYPVEEHFVTTPDGFILGLHRITGPKLFNTPPDSPPTSPR---------DLSSGNGANTIIDDIREANSNSGNRPSX--XXXXXXXXXXXSLSQSNRPVVLIMHGFMQTSEAWLCRSNPEDSLPFILADAGFDVWLGNNRGNKYSFKHTKYAPNQEGFWNWSLDELVRYDFPSIVDFITDRTQARSITYIGFSQGTAQGWATLSTNTNIAKKINLFIALAPVSTVKGFSNPMIDSLARSRPDFIFLLFGKKSMLPSTIFWRNILPKNVFVNLIDQSVGFLFGWHTENINEEEKAMLYSHIFSYTSVKSVVHWFQIIQTNRFQMFDDMLLGKTPNENSNKHNYHGRVIP-GYHPSQVLTKCALFYGGADNLPNTKALLSHIPRDKLVMVHEEEKYEHLDFMWGKDANKKIFSKIVNLINSL 471          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: UPI000644E7AC (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644E7AC)

HSP 1 Score: 266 bits (681), Expect = 2.560e-77
Identity = 163/428 (38.08%), Postives = 237/428 (55.37%), Query Frame = 2

Query:  242 RSARELVESEGFYFENHFLTTEDGFVLVLHRVIAPSGLTAKSTTAATIAAAAGNGGNSGDMGCGNGDDSCGVSVANGGSNDGVPNGGGARMGSKMASGPKNGKKVRPPILLMHGLMQDSEALLC-GGRDHSLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFEDA-----------PPGQY----IPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPR--CVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLR 1471
            R A E++ S G+  E H++TT DG++L+LHR+  P   T+   +               D+G                              S       +GK   P +L+MHG MQ SEA LC    + SL  ILA  GYDV++GNNRGN+YS K+ + +P +E++W++S+DEL R D+P++V  ++  T  +   YIGFSQG+AQ +AALS+N +L SKV L +AL+P A  +G +  ++ SL  +   F+ LLFG++ +LP+T  W+  + +  FV  +D A+ +LF W++T +SPE K  LY HI+S SSVK V HWFQ+I++ R  MF+D             P +Y    IP  Y   QI    ALF+GG DT+    +L + LPR   V  + E  +EHLD MW   A K IF K+L L++
Sbjct:   44 RDAIEMITSRGYPVEEHYVTTPDGYILMLHRITGPRAFTSPLDSPPASPR---------DLGV-----------------------------SHSELFEPSGK---PAVLIMHGFMQTSEAWLCRSDPNDSLPFILADAGYDVWLGNNRGNKYSFKHTTLSPDQEKFWNWSLDELVRYDLPSIVDFIIDRTKLKSLTYIGFSQGTAQGWAALSTNTSLASKVDLFIALAPVATVKGFSNPMIDSLARSRPDFIFLLFGKKSMLPSTTFWRNTLPKHFFVACIDWAINFLFNWQTTNLSPEEKPMLYSHIFSTSSVKSVVHWFQIIQTNRFQMFDDMLLGITPNENSNQPHRYHGRVIP-GYHPGQILTKCALFFGGADTLPNTKALLRHLPRDKVVFVHEEETYEHLDFMWAKDANKKIFSKILKLMK 429          

BLAST of mRNA_P-wetherbeei_contig1240.4.2 vs. uniprot
Match: Q86KU7_DICDI (Carboxylic ester hydrolase n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=Q86KU7_DICDI)

HSP 1 Score: 261 bits (667), Expect = 1.720e-73
Identity = 141/328 (42.99%), Postives = 206/328 (62.80%), Query Frame = 2

Query:  542 NGKKVRPPILLMHGLMQDSEALLC-GGRDHSLALILAANGYDVFVGNNRGNRYSHKNLSKAPHEERYWDFSIDELARLDVPAMVHHVLATTGAEKAVYIGFSQGSAQAFAALSSNAALQSKVALMVALSPAARAQGLAKSLLSSLVAANLRFLNLLFGRRRVLPTTLMWQRIMSRDTFVRAVDQAMLYLFTWRSTMVSPERKRQLYPHIYSYSSVKCVSHWFQMIRSRRLAMFEDA-----------PPGQY----IPVAYDITQIKIPVALFYGGRDTIVEVASLCKTLPR--CVLAYCEPDFEHLDTMWGDIAAKTIFPKVLALLR 1471
            NGK   P +++MHG MQ SEA LC      SL  ILA  G+DV++GNNRGN+YS K+ +  P++E +W++S+DEL R D P++V+ +   TGA    YIGFSQG+AQ +AALS+N  +  K+ L +AL+P A  +G +  ++ SL  +   F+ LLFG++ +LP+T+ W+  + +D FVR +D ++ +LF W +  +S + K+ LY HI+SY+SVK V HWFQ+I++ R  MF+D             P +Y    IP  Y   QI    ALFYGG D +    +L   LPR   V+ + EP +EHLD MWG  AA+ +F KV++LL+
Sbjct:  213 NGK---PVVVIMHGFMQTSEAWLCRSDPKDSLPFILADAGFDVWLGNNRGNKYSFKHTNYTPNQEGFWNWSLDELVRYDYPSIVNFICERTGAPSISYIGFSQGTAQGWAALSTNTVISKKINLFIALAPVATVKGFSNPMIDSLARSRPDFIFLLFGKKSMLPSTIFWRNFLPKDVFVRVIDTSVRFLFGWTTENISQQEKKMLYSHIFSYTSVKSVVHWFQIIQTNRFQMFDDILLGITPNENSKKPHRYHGRVIP-GYHPAQILTKCALFYGGSDNLPNTKALLSHLPRDRVVMVHEEPSYEHLDFMWGKDAAEKVFSKVVSLLK 536