mRNA_P-wetherbeei_contig52651.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig52651.1.1
Unique NamemRNA_P-wetherbeei_contig52651.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A7C4IAX5_9PROT (tRNA 2-selenouridine(34) synthase MnmH n=2 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A7C4IAX5_9PROT)

HSP 1 Score: 226 bits (576), Expect = 1.840e-70
Identity = 109/177 (61.58%), Postives = 134/177 (75.71%), Query Frame = 1 
Query:    1 RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNY 531
            R++CG TGSGKSRLL  L   GAQVLDLE++A+HRGSVLG LP + QPSQKMFE+ +  AL  FD +RP+FVE+ES+K+G ++VPD L+ RMR +PC+R++L L  RV LL+ EY H  AD   L  + DCLVPLHGRERID+WKAL     WD LV RLL EHYDPAYLR + RN+
Sbjct:  147 RVLCGTTGSGKSRLLGELSRAGAQVLDLEQLAQHRGSVLGGLPAQPQPSQKMFETRIWWALRGFDPTRPVFVESESRKVGDVRVPDALIERMRAAPCLRIELPLAERVRLLRDEYPHLEADPQALFAQLDCLVPLHGRERIDRWKALVRRGQWDALVERLLIEHYDPAYLRSIRRNF 323
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A5C9BV37_9RHOO (tRNA 2-selenouridine synthase n=1 Tax=Rhodocyclaceae bacterium TaxID=1898103 RepID=A0A5C9BV37_9RHOO)

HSP 1 Score: 226 bits (575), Expect = 2.830e-70
Identity = 108/178 (60.67%), Postives = 133/178 (74.72%), Query Frame = 1 
Query:    1 RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNYA 534
            R+VCG TG GKSR L++L   GAQVLDLE++A H GSVLG+ P   QP QK+FE+ V HAL  F A +P+FVE+ESKKIG L  P+ L+ARMR +PC+RL  D+P+RV LLK EYAHFLADA  L+ + DCL+PL GRER++ WKA + A  WDELV  LL  HYDPAY R + RNY+
Sbjct:  152 RVVCGPTGVGKSRFLKALAECGAQVLDLEDLAAHMGSVLGAYPDRPQPPQKLFETRVWHALRGFTADKPVFVESESKKIGNLHTPEPLLARMRAAPCLRLRADIPVRVTLLKEEYAHFLADAEALNRQLDCLLPLLGRERVEAWKAQARAGQWDELVADLLIHHYDPAYARSLERNYS 329
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A4R3JWM8_9PROT (tRNA 2-selenouridine synthase n=1 Tax=Sulfuritortus calidifontis TaxID=1914471 RepID=A0A4R3JWM8_9PROT)

HSP 1 Score: 225 bits (574), Expect = 3.680e-70
Identity = 108/176 (61.36%), Postives = 133/176 (75.57%), Query Frame = 1 
Query:    4 IVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNY 531
            ++CG TGSGKSR L +L   GAQ LDLE++A H+GSVLG LP   QPSQK FESL+R  L  FD +RP+FVE+ESKKIG L +PDTL+ARMR++ C+ L+ D+P RV LLK EYAHFLA+  LL+ + DCLV L G ERI++WK L+ A  WDELV  LL +HYDPAY R + RNY
Sbjct:  153 VICGRTGSGKSRFLLALREAGAQALDLEDLAAHKGSVLGGLPDRPQPSQKYFESLIRAELHDFDPARPVFVESESKKIGRLHMPDTLLARMRSAECLTLEADIPTRVALLKEEYAHFLAEPQLLNRQLDCLVDLQGHERIERWKQLALAGQWDELVAELLVDHYDPAYNRSLGRNY 328
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A0Q6SHS8_9BURK (tRNA 2-selenouridine synthase n=7 Tax=Massilia TaxID=149698 RepID=A0A0Q6SHS8_9BURK)

HSP 1 Score: 225 bits (573), Expect = 4.020e-70
Identity = 112/180 (62.22%), Postives = 134/180 (74.44%), Query Frame = 1 
Query:    1 RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNYAGY 540
            R+VCG TGSGKSRLL +L  +GAQVLDLE++A HRGSVLG LPGE QPSQKMFE+ +   L  FD +RP+FVE+ESKK+G L+VPD +M RMR +PCI L LD PLRV+LL  +Y HF AD   L+ + D LV LHGR RID W  L+N     ELV +LL EHYDPAYLR + RN+A Y
Sbjct:  146 RVVCGTTGSGKSRLLETLDAIGAQVLDLEQMAAHRGSVLGHLPGEPQPSQKMFETRIWDKLRRFDPARPVFVESESKKVGNLRVPDAVMDRMRAAPCIALQLDRPLRVQLLMEDYHHFCADPAALNAQLDHLVQLHGRARIDAWHDLANTGRMPELVDQLLVEHYDPAYLRSIDRNFAQY 325
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A7Y8MMW0_9PROT (tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Hydrogenophilaceae bacterium TaxID=2723029 RepID=A0A7Y8MMW0_9PROT)

HSP 1 Score: 223 bits (569), Expect = 2.090e-69
Identity = 107/176 (60.80%), Postives = 133/176 (75.57%), Query Frame = 1 
Query:    4 IVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNY 531
            +VCG TGSGKSR L++L   GAQVLDLE++A H+GSVLG LP   QPSQK FESL+   L  FDA RP+FVE+ESKKIG L  PDTL+ARMR++ C+ L+ D+P+RV LLK EY HFLA+  LL+ + DCLV L G +RI++WK ++ A  WDELV  LL +HYDPAY R + RNY
Sbjct:  153 VVCGRTGSGKSRFLQALREAGAQVLDLEDLAAHKGSVLGGLPDRPQPSQKYFESLIWADLHGFDADRPVFVESESKKIGRLHTPDTLLARMRSAECLTLEADIPVRVALLKEEYGHFLAEPQLLNRQLDCLVDLQGHQRIERWKQMALAGQWDELVAELLVDHYDPAYNRSLSRNY 328
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: UPI001987058F (tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Burkholderiaceae bacterium TaxID=2030806 RepID=UPI001987058F)

HSP 1 Score: 223 bits (568), Expect = 3.300e-69
Identity = 104/178 (58.43%), Postives = 137/178 (76.97%), Query Frame = 1 
Query:    1 RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNYA 534
            R++CG TGSGKSRLL+ L   GAQVLDLEE+A+HRGSVLG LP + QPSQKMFE+ +  AL TFD +RP+FVE+ES+KIG L+VP+ LM  MR S C+RL++ L +RV LL+ EY HF  D   L+++ DCL  LHG+++I QWK+L++   WD +V +LL EHYDPAYL+ + RN+A
Sbjct:  154 RVICGTTGSGKSRLLQQLAATGAQVLDLEELAQHRGSVLGGLPSQPQPSQKMFETRLWSALQTFDPARPVFVESESRKIGELRVPEGLMIAMRASECVRLEVPLEVRVRLLRDEYRHFERDLTALNDQLDCLSALHGKQKISQWKSLASDDNWDAVVEQLLVEHYDPAYLKSIDRNFA 331
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A536ZWJ3_9PROT (tRNA 2-selenouridine(34) synthase MnmH (Fragment) n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A536ZWJ3_9PROT)

HSP 1 Score: 221 bits (562), Expect = 7.680e-69
Identity = 112/176 (63.64%), Postives = 127/176 (72.16%), Query Frame = 1 
Query:    4 IVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNY 531
            +V G TGSGKSR L +LG  GAQVLDLE +A HRGSVLG LPGE QPSQK FES +  A++ FD +RP+FVE ESKKIG LQVPD LMARMR S CI L+ DL  RV LL  EY HF+AD   L+ + DCL  LHG+ERI QWKAL+ A  W E V RLL EHYDPAY R    N+
Sbjct:  135 VVHGPTGSGKSRFLAALGKAGAQVLDLEALAAHRGSVLGGLPGEPQPSQKWFESQLLAAVENFDRARPVFVEGESKKIGELQVPDALMARMRASRCITLETDLSTRVTLLLDEYRHFVADRAALEAQLDCLTALHGKERIAQWKALAAAGNWREFVTRLLIEHYDPAYRRSSMHNF 310
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A1S9AI26_9BURK (tRNA 2-selenouridine(34) synthase MnmH n=3 Tax=Massilia TaxID=149698 RepID=A0A1S9AI26_9BURK)

HSP 1 Score: 221 bits (564), Expect = 9.690e-69
Identity = 112/180 (62.22%), Postives = 132/180 (73.33%), Query Frame = 1 
Query:    1 RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNYAGY 540
            R+VCG TGSGKSRLL +L  +GAQVLDLE +A HRGSVLG LP E QP+QKMFES +   L  FD SRP+FVE+ESKK+G L+VPD +M RMR SPCI L+L  P RV LL  +Y HF AD   L+ + + LV LHGRERID W AL+N  A  ELV +LL EHYDPAYLR + RN+  Y
Sbjct:  146 RVVCGTTGSGKSRLLETLERVGAQVLDLERLAAHRGSVLGHLPDEPQPTQKMFESQIWDKLRRFDPSRPVFVESESKKVGNLRVPDAVMERMRASPCIALELSRPNRVRLLMEDYEHFCADPAALNGQLEHLVQLHGRERIDAWHALANGGAMAELVDQLLVEHYDPAYLRSIDRNFVHY 325
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A7V9DXH5_9BURK (tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Burkholderiaceae bacterium TaxID=2030806 RepID=A0A7V9DXH5_9BURK)

HSP 1 Score: 219 bits (559), Expect = 4.510e-68
Identity = 103/178 (57.87%), Postives = 132/178 (74.16%), Query Frame = 1 
Query:    1 RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNYA 534
            R++CG TGSGKSRLL+ L  LGAQVLDLE +A HRGSVLG LP   QPSQKMFE+ +  AL  FD + P+F+E+ES+KIG L++PD L+A MR S CIRL++ L  R+ LL+ EY H+  D   LD + DCL  LHG+++I QWKAL+N   WD +V RLL +HYDPAYL+ + RN+A
Sbjct:  141 RVICGTTGSGKSRLLQQLSALGAQVLDLEHLALHRGSVLGGLPSHPQPSQKMFETRLWSALRAFDPAYPVFIESESRKIGELRIPDPLLAAMRASECIRLEVPLAARIRLLREEYEHYERDRGALDSQLDCLTALHGKQKISQWKALANDDNWDGVVERLLLDHYDPAYLKSIDRNFA 318
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BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Match: A0A258TRA6_9PROT (tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Hydrogenophilales bacterium 28-61-23 TaxID=1970530 RepID=A0A258TRA6_9PROT)

HSP 1 Score: 219 bits (558), Expect = 9.750e-68
Identity = 103/179 (57.54%), Postives = 127/179 (70.95%), Query Frame = 1 
Query:    1 RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQKMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRLDLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNAHAWDELVLRLLTEHYDPAYLRGMPRNYAG 537
            R++CG TG GKSR LR L    AQVLDLE++A H GSVLG+ P   QP+QK+FES V H L +FD  RPIFVE+ESKKIG L  P+ L+ RMR + CI L  D+P+RVELLK EYAHFL DA  L ++ DCL  + G+E+++ WK L+ +  WDELV  LL  HYDPAY R + RNY G
Sbjct:  153 RVICGPTGVGKSRFLRELKACKAQVLDLEDLAAHMGSVLGAYPARPQPAQKLFESRVWHTLQSFDPERPIFVESESKKIGNLHTPEELLKRMRAATCINLHADIPVRVELLKQEYAHFLIDAESLGQKLDCLTTIQGKEQVENWKVLARSGRWDELVAELLIRHYDPAYTRSLDRNYVG 331
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig52651.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7C4IAX5_9PROT1.840e-7061.58tRNA 2-selenouridine(34) synthase MnmH n=2 Tax=Bet... [more]
A0A5C9BV37_9RHOO2.830e-7060.67tRNA 2-selenouridine synthase n=1 Tax=Rhodocyclace... [more]
A0A4R3JWM8_9PROT3.680e-7061.36tRNA 2-selenouridine synthase n=1 Tax=Sulfuritortu... [more]
A0A0Q6SHS8_9BURK4.020e-7062.22tRNA 2-selenouridine synthase n=7 Tax=Massilia Tax... [more]
A0A7Y8MMW0_9PROT2.090e-6960.80tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Hyd... [more]
UPI001987058F3.300e-6958.43tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Bur... [more]
A0A536ZWJ3_9PROT7.680e-6963.64tRNA 2-selenouridine(34) synthase MnmH (Fragment) ... [more]
A0A1S9AI26_9BURK9.690e-6962.22tRNA 2-selenouridine(34) synthase MnmH n=3 Tax=Mas... [more]
A0A7V9DXH5_9BURK4.510e-6857.87tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Bur... [more]
A0A258TRA6_9PROT9.750e-6857.54tRNA 2-selenouridine(34) synthase MnmH n=1 Tax=Hyd... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig52651contigP-wetherbeei_contig52651:1..540 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog883126.HMPREF9710_04988
Preferred nameselU
PFAMsRhodanese
Model size540
Max annot lvl28216|Betaproteobacteria
KEGG koko:K06917
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.69e-66
EggNOG OGsCOG2603@1|root,COG2603@2|Bacteria,1N4T5@1224|Proteobacteria,2VIMH@28216|Betaproteobacteria,472FU@75682|Oxalobacteraceae
DescriptionCatalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA
Cds size540
COG categoryH
BRITEko00000,ko01000,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186788.7166858-CDS-P-wetherbeei_contig52651:0..5401683186788.7166858-CDS-P-wetherbeei_contig52651:0..540Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig52651 1..540 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig52651.1.1prot_P-wetherbeei_contig52651.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig52651 1..540 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig52651.1.1

>prot_P-wetherbeei_contig52651.1.1 ID=prot_P-wetherbeei_contig52651.1.1|Name=mRNA_P-wetherbeei_contig52651.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=180bp
RIVCGVTGSGKSRLLRSLGTLGAQVLDLEEIARHRGSVLGSLPGEMQPSQ
KMFESLVRHALDTFDASRPIFVEAESKKIGALQVPDTLMARMRTSPCIRL
DLDLPLRVELLKGEYAHFLADAPLLDERFDCLVPLHGRERIDQWKALSNA
HAWDELVLRLLTEHYDPAYLRGMPRNYAGY
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mRNA from alignment at P-wetherbeei_contig52651:1..540+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig52651.1.1 ID=mRNA_P-wetherbeei_contig52651.1.1|Name=mRNA_P-wetherbeei_contig52651.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=540bp|location=Sequence derived from alignment at P-wetherbeei_contig52651:1..540+ (Phaeothamnion wetherbeei SAG_119_79)
CGCATCGTTTGCGGGGTTACCGGCAGTGGCAAGAGCCGCCTGCTGCGCTC
GCTCGGTACGCTTGGCGCGCAGGTGCTGGACTTGGAAGAGATCGCCCGGC
ATCGCGGCTCGGTGCTGGGTTCGCTGCCTGGCGAGATGCAGCCTTCGCAG
AAAATGTTTGAAAGCCTGGTGCGCCACGCGCTCGACACTTTCGATGCGTC
ACGCCCGATTTTTGTCGAAGCCGAGAGCAAGAAGATCGGCGCCCTGCAGG
TGCCGGACACCCTGATGGCACGCATGCGTACTTCGCCCTGCATACGCCTT
GACCTTGACCTGCCGCTGCGGGTGGAATTGCTCAAGGGGGAGTATGCGCA
CTTCCTTGCCGATGCGCCGTTGCTGGACGAGCGCTTCGACTGCCTTGTGC
CCTTGCATGGGCGCGAGCGAATCGACCAGTGGAAGGCGCTATCGAACGCG
CACGCCTGGGATGAACTGGTGTTGCGCCTGCTGACCGAACATTACGACCC
GGCCTACCTGCGTGGAATGCCGCGCAATTACGCCGGCTAC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig52651:1..540+

>mRNA_P-wetherbeei_contig52651.1.1 ID=mRNA_P-wetherbeei_contig52651.1.1|Name=mRNA_P-wetherbeei_contig52651.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=540bp|location=Sequence derived from alignment at P-wetherbeei_contig52651:1..540+ (Phaeothamnion wetherbeei SAG_119_79)
CGCATCGTTTGCGGGGTTACCGGCAGTGGCAAGAGCCGCCTGCTGCGCTC
GCTCGGTACGCTTGGCGCGCAGGTGCTGGACTTGGAAGAGATCGCCCGGC
ATCGCGGCTCGGTGCTGGGTTCGCTGCCTGGCGAGATGCAGCCTTCGCAG
AAAATGTTTGAAAGCCTGGTGCGCCACGCGCTCGACACTTTCGATGCGTC
ACGCCCGATTTTTGTCGAAGCCGAGAGCAAGAAGATCGGCGCCCTGCAGG
TGCCGGACACCCTGATGGCACGCATGCGTACTTCGCCCTGCATACGCCTT
GACCTTGACCTGCCGCTGCGGGTGGAATTGCTCAAGGGGGAGTATGCGCA
CTTCCTTGCCGATGCGCCGTTGCTGGACGAGCGCTTCGACTGCCTTGTGC
CCTTGCATGGGCGCGAGCGAATCGACCAGTGGAAGGCGCTATCGAACGCG
CACGCCTGGGATGAACTGGTGTTGCGCCTGCTGACCGAACATTACGACCC
GGCCTACCTGCGTGGAATGCCGCGCAATTACGCCGGCTAC
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