mRNA_P-wetherbeei_contig20146.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig20146.2.1
Unique NamemRNA_P-wetherbeei_contig20146.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: A0A2E2BCJ6_9GAMM (Dual-specificity RNA methyltransferase RlmN n=1 Tax=Thiotrichales bacterium TaxID=2026796 RepID=A0A2E2BCJ6_9GAMM)

HSP 1 Score: 238 bits (606), Expect = 3.990e-75
Identity = 117/169 (69.23%), Postives = 138/169 (81.66%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQAR 507
            M+D+NAYNL +KR+TLSTAG+VPGIDKLA  CPVSLAISLHATN+ LRDKLVP+N+ YPI  LL  CK Y  +     ITFEYVMLKDVNDSV EA  LI+LL G+PAKVNLIPFNP+ ++ FERS   AIN FRD+L ++GI+T+TRKTRG DI AACGQLVG+V+ R
Sbjct:  183 MMDNNAYNLGKKRITLSTAGIVPGIDKLAEECPVSLAISLHATNNYLRDKLVPLNKKYPIERLLETCKNYSRATNDNYITFEYVMLKDVNDSVKEASVLIKLLDGIPAKVNLIPFNPYQNSSFERSEERAINDFRDKLYKAGIITITRKTRGSDIDAACGQLVGQVEKR 351
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: A0A351MFP7_9GAMM (Dual-specificity RNA methyltransferase RlmN n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A351MFP7_9GAMM)

HSP 1 Score: 236 bits (602), Expect = 4.000e-74
Identity = 120/172 (69.77%), Postives = 140/172 (81.40%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            M+DD AY LARKRVTLSTAG+VP ID+L     VSLA+SLHAT+D LRD+L+PINR+YPIA+LLAAC+RY T +PH  ITFEYVML  VNDS A ARAL RLL+G+PAKVNLIPFNPFA AGF RS    I+AFR+ L   G++ +TR+TRGDDI AACGQLVG+V ARA R
Sbjct:  198 MMDDCAYGLARKRVTLSTAGIVPAIDRLREVSGVSLAVSLHATDDALRDRLMPINRTYPIAELLAACRRYATLDPHRSITFEYVMLDGVNDSPAHARALARLLRGMPAKVNLIPFNPFAGAGFRRSPQPVIDAFREVLRALGLICITRRTRGDDIDAACGQLVGKVMARAAR 369
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: A0A523P2N2_9PROT (Dual-specificity RNA methyltransferase RlmN n=1 Tax=Proteobacteria bacterium TaxID=1977087 RepID=A0A523P2N2_9PROT)

HSP 1 Score: 234 bits (598), Expect = 7.880e-74
Identity = 116/172 (67.44%), Postives = 139/172 (80.81%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            M+DD+AYNLARKRVTLSTAG VPGI KL   CPVSLA+SLHA NDELR +LVPIN+ YP+  L+ AC++Y  +NPH  ITFEYVMLK +ND  + AR L+RLL G+PAKVNLIPFNP+    +E S+ D I+ FRD+L RSGIVT+TRKTRGDDI AACGQL+G+VQA + R
Sbjct:  183 MMDDHAYNLARKRVTLSTAGHVPGIQKLRNVCPVSLAVSLHAPNDELRSELVPINKKYPLEALMDACRQYADTNPHDHITFEYVMLKGINDDPSLARDLVRLLHGIPAKVNLIPFNPYPGISYECSSRDIIDRFRDQLVRSGIVTITRKTRGDDIDAACGQLIGKVQATSAR 354
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: A0A1F6TGY0_9PROT (Dual-specificity RNA methyltransferase RlmN n=3 Tax=Candidatus Muproteobacteria TaxID=1817795 RepID=A0A1F6TGY0_9PROT)

HSP 1 Score: 233 bits (594), Expect = 3.170e-73
Identity = 116/172 (67.44%), Postives = 139/172 (80.81%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            MLDD AY L+R+RVT+STAGVVP +D+L A CPVSLA+SLHAT+D LRD+LVP+NR YPI +LLAACKRYV  +    +TFEYVML  VND VA+ARAL+RLL+G+PAKVNLIPFNPF    +  S  +AI+ FRD L ++G++TVTRKTRGDDIAAACGQL G V  RA R
Sbjct:  184 MLDDLAYGLSRRRVTVSTAGVVPMMDRLRATCPVSLAVSLHATDDALRDELVPLNRKYPIRELLAACKRYVAGDARRRVTFEYVMLAGVNDDVAQARALVRLLEGIPAKVNLIPFNPFPHTSYRCSAPEAIDRFRDALMQAGLMTVTRKTRGDDIAAACGQLAGRVLERAPR 355
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: UPI0019CAAC2D (Radical SAM protein n=1 Tax=Burkholderiales bacterium TaxID=1891238 RepID=UPI0019CAAC2D)

HSP 1 Score: 224 bits (571), Expect = 5.000e-72
Identity = 110/172 (63.95%), Postives = 135/172 (78.49%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            MLDD+AY L+R+RVTLST+G+VP ID+L+  CPV+LA+SLHA ND LRD LVPINR YP+  LLAAC RY+   P   ITFEYVMLK +NDS+  AR LI L++ +P K+NLIPFNPFA +GF+RS  ++I  F D L  +G+V  TRKTRGDDIAAACGQL GEVQ R++R
Sbjct:    9 MLDDDAYGLSRRRVTLSTSGLVPSIDRLSEACPVALAVSLHAPNDTLRDVLVPINRKYPLRQLLAACSRYLEKAPRDFITFEYVMLKGINDSLQHARELIELVRDVPCKLNLIPFNPFAGSGFDRSPAESIRRFADVLLNAGLVVTTRKTRGDDIAAACGQLAGEVQDRSKR 180
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: RLMN_NITMU (Dual-specificity RNA methyltransferase RlmN n=8 Tax=Nitrosomonadales TaxID=32003 RepID=RLMN_NITMU)

HSP 1 Score: 229 bits (584), Expect = 9.920e-72
Identity = 109/172 (63.37%), Postives = 139/172 (80.81%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            MLDDNAY L+R+RVT+ST+G++P +D+L   CPV+LA+SLHA ND LRD+LVPINR YPI +LL AC+RY+ S P   ITFEYVML  VNDSVA+AR L++L++ +P K+NLIPFNPF D+GF RS+ +A++ FRD L  +G+VT  RKTRGDDIAAACGQL G+V  + RR
Sbjct:  184 MLDDNAYGLSRRRVTVSTSGIIPAMDRLRERCPVALAVSLHAPNDALRDQLVPINRKYPIRELLGACERYLQSAPRDFITFEYVMLDGVNDSVAQARELVQLVRDIPCKLNLIPFNPFPDSGFRRSSANAVSRFRDVLMEAGLVTTVRKTRGDDIAAACGQLAGKVLDKTRR 355
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: A0A7C1UG04_9GAMM (Dual-specificity RNA methyltransferase RlmN n=1 Tax=Acidiferrobacteraceae bacterium TaxID=2024893 RepID=A0A7C1UG04_9GAMM)

HSP 1 Score: 229 bits (583), Expect = 1.360e-71
Identity = 113/172 (65.70%), Postives = 136/172 (79.07%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            MLDD AY L+R+RVT+STAGVVPGID+LA  CPVSLA+SLHA NDELRD+LVP+NR YPI  LLAAC+RYV   P + +TFEY ML  VNDS  +AR L+ +L  +PAKVNLIPFNPF  +G++RS    I+ FR+ L R G+VT+TRKTRG+DI AACGQL G+VQ R RR
Sbjct:  183 MLDDMAYGLSRRRVTISTAGVVPGIDRLAEVCPVSLAVSLHAPNDELRDQLVPLNRKYPIVPLLAACRRYVRHQPRSRVTFEYTMLDGVNDSDTQARQLLGILGDVPAKVNLIPFNPFPGSGYQRSGEQTIDRFREILHRGGVVTMTRKTRGEDIDAACGQLAGKVQDRTRR 354
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: D5BZ51_NITHN (Dual-specificity RNA methyltransferase RlmN n=3 Tax=Nitrosococcus TaxID=1227 RepID=D5BZ51_NITHN)

HSP 1 Score: 228 bits (582), Expect = 2.420e-71
Identity = 114/172 (66.28%), Postives = 137/172 (79.65%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            MLDD +Y L+ +RVTLSTAG+VP +D+L A CPVSLA+SLHA  DELRD+LVP+N+ YPI +LLAAC+RYV  +    +TFEYVML  VNDS+  ARAL+RLLQGLPAKVNLIPFNPF  + + RS  + I+ FR+ L R G++TVTRKTRGDDIAAACGQL G VQ R RR
Sbjct:  184 MLDDFSYGLSWRRVTLSTAGMVPAMDRLRAICPVSLAVSLHAPTDELRDELVPLNKRYPIGELLAACRRYVAGDRRRAVTFEYVMLAGVNDSLQHARALLRLLQGLPAKVNLIPFNPFPGSLYRRSDTETIDRFREELLRGGLMTVTRKTRGDDIAAACGQLAGRVQDRTRR 355
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: A0A286C088_9PROT (Dual-specificity RNA methyltransferase RlmN n=2 Tax=unclassified Nitrosovibrio TaxID=2624428 RepID=A0A286C088_9PROT)

HSP 1 Score: 228 bits (580), Expect = 3.980e-71
Identity = 108/172 (62.79%), Postives = 138/172 (80.23%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            MLDDNAY L+R+RVT+ST+G+VP +D+L   CPV+LA+SLHA ND LRD+LVPIN+ YPI +LLAAC+RY+ S P   ITFEYVML  VNDSVA A+ L++L++ +P K NLIPFNPF D+G+ RS+ DA+  FRD L ++G++T  RKTRGDDIAAACGQL G+V  + RR
Sbjct:  184 MLDDNAYGLSRRRVTVSTSGLVPAMDRLRQRCPVALAVSLHAPNDALRDQLVPINKKYPIRELLAACERYLESAPRDFITFEYVMLDGVNDSVAHAQELVKLVRHIPCKFNLIPFNPFPDSGYRRSSADAVRCFRDVLMQAGLITTVRKTRGDDIAAACGQLAGKVHDKTRR 355
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BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Match: A0A7V8ZPJ6_9BURK (Dual-specificity RNA methyltransferase RlmN n=1 Tax=Burkholderiales bacterium TaxID=1891238 RepID=A0A7V8ZPJ6_9BURK)

HSP 1 Score: 227 bits (578), Expect = 1.090e-70
Identity = 111/172 (64.53%), Postives = 135/172 (78.49%), Query Frame = 1 
Query:    1 MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDKLVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALIRLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKTRGDDIAAACGQLVGEVQARARR 516
            MLDDNAY L+R+RVT+ST+G+VP ID+L   CPV+LA+SLHA ND+LRD LVPINR YP+  LLAAC RY+   P   ITFEYVMLK +NDSV  AR LI L++ +P K+NLIPFNPFA +G+ERS  ++I  F D L  +G+V  TRKTRGDDIAAACGQL GEVQ R++R
Sbjct:  189 MLDDNAYGLSRRRVTVSTSGIVPAIDRLRDQCPVALAVSLHAPNDKLRDVLVPINRKYPLQQLLAACSRYLERAPRDFITFEYVMLKGINDSVQHARELIGLVRDVPCKLNLIPFNPFAGSGYERSPAESIRRFADALMNAGLVATTRKTRGDDIAAACGQLAGEVQDRSQR 360
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig20146.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E2BCJ6_9GAMM3.990e-7569.23Dual-specificity RNA methyltransferase RlmN n=1 Ta... [more]
A0A351MFP7_9GAMM4.000e-7469.77Dual-specificity RNA methyltransferase RlmN n=1 Ta... [more]
A0A523P2N2_9PROT7.880e-7467.44Dual-specificity RNA methyltransferase RlmN n=1 Ta... [more]
A0A1F6TGY0_9PROT3.170e-7367.44Dual-specificity RNA methyltransferase RlmN n=3 Ta... [more]
UPI0019CAAC2D5.000e-7263.95Radical SAM protein n=1 Tax=Burkholderiales bacter... [more]
RLMN_NITMU9.920e-7263.37Dual-specificity RNA methyltransferase RlmN n=8 Ta... [more]
A0A7C1UG04_9GAMM1.360e-7165.70Dual-specificity RNA methyltransferase RlmN n=1 Ta... [more]
D5BZ51_NITHN2.420e-7166.28Dual-specificity RNA methyltransferase RlmN n=3 Ta... [more]
A0A286C088_9PROT3.980e-7162.79Dual-specificity RNA methyltransferase RlmN n=2 Ta... [more]
A0A7V8ZPJ6_9BURK1.090e-7064.53Dual-specificity RNA methyltransferase RlmN n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig20146contigP-wetherbeei_contig20146:667..1182 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog105559.Nwat_0767
Preferred namerlmN
PFAMsRadical_SAM
Model size516
Max annot lvl135613|Chromatiales
KEGG koko:K06941
Hectar predicted targeting categoryother localisation
Exons1
Evalue3.08e-74
EggNOG OGsCOG0820@1|root,COG0820@2|Bacteria,1MUYK@1224|Proteobacteria,1RMUI@1236|Gammaproteobacteria,1WWD4@135613|Chromatiales
EC2.1.1.192
DescriptionSpecifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs
Cds size516
COG categoryJ
BRITEko00000,ko01000,ko03009
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186192.300174-CDS-P-wetherbeei_contig20146:666..11821683186192.300174-CDS-P-wetherbeei_contig20146:666..1182Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig20146 667..1182 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig20146.2.1prot_P-wetherbeei_contig20146.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig20146 667..1182 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig20146.2.1

>prot_P-wetherbeei_contig20146.2.1 ID=prot_P-wetherbeei_contig20146.2.1|Name=mRNA_P-wetherbeei_contig20146.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=172bp
MLDDNAYNLARKRVTLSTAGVVPGIDKLAAHCPVSLAISLHATNDELRDK
LVPINRSYPIADLLAACKRYVTSNPHAEITFEYVMLKDVNDSVAEARALI
RLLQGLPAKVNLIPFNPFADAGFERSTGDAINAFRDRLSRSGIVTVTRKT
RGDDIAAACGQLVGEVQARARR
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mRNA from alignment at P-wetherbeei_contig20146:667..1182-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig20146.2.1 ID=mRNA_P-wetherbeei_contig20146.2.1|Name=mRNA_P-wetherbeei_contig20146.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=516bp|location=Sequence derived from alignment at P-wetherbeei_contig20146:667..1182- (Phaeothamnion wetherbeei SAG_119_79)
ATGCTCGACGACAATGCCTACAATCTTGCGCGCAAGCGCGTGACTTTAAG
CACCGCCGGTGTGGTGCCGGGCATCGACAAACTCGCCGCCCATTGCCCGG
TCAGCCTCGCAATTTCGCTGCACGCGACCAATGATGAACTGCGCGACAAA
CTGGTGCCGATCAACCGCAGCTATCCCATCGCTGATCTGCTGGCGGCCTG
CAAACGCTATGTCACCAGCAACCCGCACGCCGAAATCACGTTTGAATATG
TGATGTTGAAGGATGTTAACGACAGTGTCGCCGAAGCGCGCGCCCTGATT
CGACTGCTGCAGGGACTGCCGGCCAAGGTCAACCTGATCCCGTTCAATCC
CTTTGCCGACGCCGGCTTCGAGCGGTCCACCGGCGATGCGATCAACGCCT
TCCGTGATCGACTGTCACGTTCCGGCATCGTCACGGTCACACGCAAGACA
CGCGGCGACGACATCGCGGCAGCCTGCGGACAACTGGTCGGTGAAGTCCA
GGCCCGCGCGCGCCGT
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Coding sequence (CDS) from alignment at P-wetherbeei_contig20146:667..1182-

>mRNA_P-wetherbeei_contig20146.2.1 ID=mRNA_P-wetherbeei_contig20146.2.1|Name=mRNA_P-wetherbeei_contig20146.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=516bp|location=Sequence derived from alignment at P-wetherbeei_contig20146:667..1182- (Phaeothamnion wetherbeei SAG_119_79)
ATGCTCGACGACAATGCCTACAATCTTGCGCGCAAGCGCGTGACTTTAAG
CACCGCCGGTGTGGTGCCGGGCATCGACAAACTCGCCGCCCATTGCCCGG
TCAGCCTCGCAATTTCGCTGCACGCGACCAATGATGAACTGCGCGACAAA
CTGGTGCCGATCAACCGCAGCTATCCCATCGCTGATCTGCTGGCGGCCTG
CAAACGCTATGTCACCAGCAACCCGCACGCCGAAATCACGTTTGAATATG
TGATGTTGAAGGATGTTAACGACAGTGTCGCCGAAGCGCGCGCCCTGATT
CGACTGCTGCAGGGACTGCCGGCCAAGGTCAACCTGATCCCGTTCAATCC
CTTTGCCGACGCCGGCTTCGAGCGGTCCACCGGCGATGCGATCAACGCCT
TCCGTGATCGACTGTCACGTTCCGGCATCGTCACGGTCACACGCAAGACA
CGCGGCGACGACATCGCGGCAGCCTGCGGACAACTGGTCGGTGAAGTCCA
GGCCCGCGCGCGCCGT
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