mRNA_P-wetherbeei_contig1637.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig1637.1.1
Unique NamemRNA_P-wetherbeei_contig1637.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A835YXB1_9STRA (Fatty acid desaturase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YXB1_9STRA)

HSP 1 Score: 207 bits (527), Expect = 3.180e-63
Identity = 89/113 (78.76%), Postives = 102/113 (90.27%), Query Frame = 1 
Query:   13 VLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSRS 351
            VLIT+LQHTDVF+PHFRG EW WFRGALCTVDRSFGA IDHTIHHI+DTHVCHHLFSKMPFY+AQEAT  +R+ LG++YL D TP+PQALWR+YTCC F+ED+G  VFYKSR+
Sbjct:  325 VLITYLQHTDVFMPHFRGDEWSWFRGALCTVDRSFGALIDHTIHHIADTHVCHHLFSKMPFYNAQEATEILREKLGEYYLSDATPIPQALWRSYTCCAFVEDEGSAVFYKSRA 437
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A482S9L4_9ARCH (FA_desaturase domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S9L4_9ARCH)

HSP 1 Score: 198 bits (503), Expect = 2.150e-60
Identity = 85/111 (76.58%), Postives = 99/111 (89.19%), Query Frame = 1 
Query:   13 VLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKS 345
            VLIT+LQHTDV++PHFR KEW W RGALCTVDRSFGA +DHT HHI+DTHVCHHLFSKMPFYHA EAT AI+KV G +YL+DDTP+ +ALWR+Y+ C FIED+GDIVFYK+
Sbjct:  256 VLITYLQHTDVYMPHFRNKEWTWLRGALCTVDRSFGAILDHTFHHITDTHVCHHLFSKMPFYHATEATAAIKKVCGKYYLKDDTPILKALWRSYSTCQFIEDEGDIVFYKN 366
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A1S7C7Q6_9STRA (Delta-12 fatty acid desaturase n=1 Tax=Nannochloropsis oceanica TaxID=145522 RepID=A0A1S7C7Q6_9STRA)

HSP 1 Score: 194 bits (492), Expect = 5.390e-58
Identity = 83/117 (70.94%), Postives = 99/117 (84.62%), Query Frame = 1 
Query:    1 NHKQVLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSRS 351
            N+  VLIT+LQHTDVF+PHFRG EW WFRGALCTVDRSFG  +DHT HHISDTHVCHH+FSKMPFYHAQEA+  IRK LGD+YL+DDTP+ +ALWR+YT C +++ +   VFYK R+
Sbjct:  322 NYHLVLITYLQHTDVFIPHFRGSEWTWFRGALCTVDRSFGWLLDHTFHHISDTHVCHHIFSKMPFYHAQEASEHIRKALGDYYLKDDTPIWKALWRSYTLCKYVDSEETTVFYKQRA 438
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A6H5K1W8_9PHAE (SAP domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K1W8_9PHAE)

HSP 1 Score: 192 bits (487), Expect = 3.440e-57
Identity = 82/112 (73.21%), Postives = 96/112 (85.71%), Query Frame = 1 
Query:   13 VLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSR 348
            VLIT+LQHTDVF+PHFRG EW+WFRGALCTVDR+FG  IDH IHHI+DTHVCHHLFSKMPFYHAQEAT  IR  LGD++L D TP+P+ALWR++TCC  +ED G  VFY++ 
Sbjct:  333 VLITYLQHTDVFMPHFRGSEWDWFRGALCTVDRTFGPVIDHVIHHIADTHVCHHLFSKMPFYHAQEATEHIRAFLGDYHLSDTTPIPKALWRSFTCCTHVEDVGHTVFYQAH 444
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A4D9CZV5_9STRA (FA_desaturase domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CZV5_9STRA)

HSP 1 Score: 187 bits (476), Expect = 4.310e-57
Identity = 81/117 (69.23%), Postives = 98/117 (83.76%), Query Frame = 1 
Query:    1 NHKQVLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSRS 351
            N+  VLIT+LQHTDVF+PHFRG EW W RGALCTVDRSFG  +DHT HHISDTHVCHH+FSK+PFYHAQEA+  I+KVLG +YL+DDTP+ +ALWR+ T C +++ D D VFYK R+
Sbjct:  186 NYHLVLITYLQHTDVFIPHFRGTEWSWLRGALCTVDRSFGWLLDHTFHHISDTHVCHHIFSKIPFYHAQEASEHIKKVLGPYYLKDDTPIWKALWRSCTLCKYVDTDKDAVFYKHRA 302
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A8K1CR19_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CR19_PYTOL)

HSP 1 Score: 190 bits (483), Expect = 5.340e-57
Identity = 82/112 (73.21%), Postives = 96/112 (85.71%), Query Frame = 1 
Query:   13 VLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSR 348
            VLIT+LQHTD ++PHFR  EW W RGALCTVDRSFG+F+D  +HHI DTHVCHH+FSKMPFYHAQEAT A++ VLG FYL+DDT +P+ALWR+Y  C FIEDDG IVFY+SR
Sbjct:  290 VLITYLQHTDTYVPHFREGEWSWLRGALCTVDRSFGSFLDSVLHHIVDTHVCHHIFSKMPFYHAQEATEALKPVLGRFYLKDDTSIPRALWRSYNLCKFIEDDGKIVFYRSR 401
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A5D6Y309_9STRA (FA_desaturase domain-containing protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Y309_9STRA)

HSP 1 Score: 190 bits (483), Expect = 5.740e-57
Identity = 81/111 (72.97%), Postives = 96/111 (86.49%), Query Frame = 1 
Query:   13 VLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKS 345
            VLIT+LQHTD F+PHFR  EW W RGALCTVDRSFGA++D  +HHI DTHV HH+FSKMPFYHA+EAT A++ +LG FYL+DDTP+PQALWR+YT C F+EDDG +VFYKS
Sbjct:  295 VLITYLQHTDTFVPHFRDGEWSWLRGALCTVDRSFGAYLDGVLHHIVDTHVAHHIFSKMPFYHAEEATAALKPLLGRFYLKDDTPIPQALWRSYTHCKFVEDDGTVVFYKS 405
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: K8YR13_NANGC (Omega-6 fatty acid desaturase delta-12 n=3 Tax=Monodopsidaceae TaxID=425072 RepID=K8YR13_NANGC)

HSP 1 Score: 191 bits (486), Expect = 5.800e-57
Identity = 82/117 (70.09%), Postives = 99/117 (84.62%), Query Frame = 1 
Query:    1 NHKQVLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSRS 351
            N+  VLIT+LQHTDVF+PHFRG EW WFRGALCTVDRSFG  +DHT HHISDTHVCHH+FSKMPFYHAQEA+  I+K LG +YL+DDTP+ +ALWR+YT C +++ D + VFYK R+
Sbjct:  335 NYHLVLITYLQHTDVFIPHFRGAEWSWFRGALCTVDRSFGWLLDHTFHHISDTHVCHHIFSKMPFYHAQEASEHIKKALGPYYLKDDTPIWKALWRSYTLCKYVDTDKNAVFYKHRA 451
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A662XTH7_9STRA (FA_desaturase domain-containing protein n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662XTH7_9STRA)

HSP 1 Score: 192 bits (487), Expect = 8.060e-57
Identity = 80/112 (71.43%), Postives = 96/112 (85.71%), Query Frame = 1 
Query:   13 VLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSR 348
            VLIT+LQHTD ++PHFR  EW W RGALCTVDRSFG+++DH +H I DTHVCHH+FSKMPFYH +EAT AI+ +LG FYL+D TPVP ALWR+YTCC FIEDDG +VFYK++
Sbjct:  371 VLITYLQHTDTYIPHFREGEWNWLRGALCTVDRSFGSYLDHVVHRIVDTHVCHHIFSKMPFYHCEEATNAIKPLLGKFYLKDSTPVPLALWRSYTCCKFIEDDGKVVFYKNK 482
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BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Match: A0A3R7HN64_9STRA (FA_desaturase domain-containing protein n=5 Tax=Phytophthora TaxID=4783 RepID=A0A3R7HN64_9STRA)

HSP 1 Score: 188 bits (478), Expect = 4.070e-56
Identity = 79/112 (70.54%), Postives = 95/112 (84.82%), Query Frame = 1 
Query:   13 VLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHISDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTCCGFIEDDGDIVFYKSR 348
            VLIT+LQHTD ++PHFR  EW W RGALCTVDRSFG+++DH +H I DTHVCHH+FSKMPFYH +EAT AI+ +LG FYL+D TPVP ALWR+YT C F+EDDG IVFYK++
Sbjct:  286 VLITYLQHTDTYIPHFREGEWNWLRGALCTVDRSFGSYLDHVVHRIVDTHVCHHIFSKMPFYHCEEATNAIKPILGKFYLKDTTPVPLALWRSYTTCKFVEDDGKIVFYKNK 397
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig1637.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YXB1_9STRA3.180e-6378.76Fatty acid desaturase n=1 Tax=Tribonema minus TaxI... [more]
A0A482S9L4_9ARCH2.150e-6076.58FA_desaturase domain-containing protein n=1 Tax=ar... [more]
A0A1S7C7Q6_9STRA5.390e-5870.94Delta-12 fatty acid desaturase n=1 Tax=Nannochloro... [more]
A0A6H5K1W8_9PHAE3.440e-5773.21SAP domain-containing protein n=2 Tax=Ectocarpus T... [more]
A0A4D9CZV5_9STRA4.310e-5769.23FA_desaturase domain-containing protein n=1 Tax=Na... [more]
A0A8K1CR19_PYTOL5.340e-5773.21Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A5D6Y309_9STRA5.740e-5772.97FA_desaturase domain-containing protein n=1 Tax=Py... [more]
K8YR13_NANGC5.800e-5770.09Omega-6 fatty acid desaturase delta-12 n=3 Tax=Mon... [more]
A0A662XTH7_9STRA8.060e-5771.43FA_desaturase domain-containing protein n=2 Tax=No... [more]
A0A3R7HN64_9STRA4.070e-5670.54FA_desaturase domain-containing protein n=5 Tax=Ph... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig1637contigP-wetherbeei_contig1637:101..460 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog2880.D7FQQ2
Preferred nameFAD2
PFAMsDUF3474,FA_desaturase
Model size360
Max annot lvl2759|Eukaryota
KEGG rclassRC00917
KEGG koko:K10256,ko:K21704,ko:K21710,ko:K21736
KEGG ReactionR11043,R11044
KEGG Pathwayko01040,ko01212,map01040,map01212
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004497,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0008150,GO:0008152,GO:0012505,GO:0016020,GO:0016021,GO:0016491,GO:0016705,GO:0016709,GO:0030176,GO:0031224,GO:0031227,GO:0031984,GO:0042175,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045485,GO:0050183,GO:0055114,GO:0098827
Exons1
Evalue5.64e-58
EggNOG OGsCOG3239@1|root,2QQNB@2759|Eukaryota
Ec32 ortholog descriptionSAP domain
Ec32 orthologEc-01_000100.1
EC1.14.18.4,1.14.19.16,1.14.19.22,1.14.19.33,1.14.19.34,1.14.19.6
Descriptionoxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
Cds size360
COG categoryI
BRITEko00000,ko00001,ko01000,ko01004
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186084.7645454-CDS-P-wetherbeei_contig1637:100..4601683186084.7645454-CDS-P-wetherbeei_contig1637:100..460Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1637 101..460 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig1637.1.1prot_P-wetherbeei_contig1637.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig1637 101..460 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig1637.1.1

>prot_P-wetherbeei_contig1637.1.1 ID=prot_P-wetherbeei_contig1637.1.1|Name=mRNA_P-wetherbeei_contig1637.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=120bp
NHKQVLITFLQHTDVFLPHFRGKEWEWFRGALCTVDRSFGAFIDHTIHHI
SDTHVCHHLFSKMPFYHAQEATLAIRKVLGDFYLRDDTPVPQALWRAYTC
CGFIEDDGDIVFYKSRSGL*
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mRNA from alignment at P-wetherbeei_contig1637:101..460+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig1637.1.1 ID=mRNA_P-wetherbeei_contig1637.1.1|Name=mRNA_P-wetherbeei_contig1637.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=360bp|location=Sequence derived from alignment at P-wetherbeei_contig1637:101..460+ (Phaeothamnion wetherbeei SAG_119_79)
AACCATAAACAGGTGCTGATCACGTTTCTGCAGCACACGGACGTGTTCCT
GCCACACTTCCGCGGCAAGGAGTGGGAGTGGTTCCGCGGCGCGCTCTGCA
CCGTCGACCGATCCTTCGGCGCCTTCATCGACCACACCATCCACCACATC
TCCGACACGCATGTGTGCCACCACCTCTTCAGCAAGATGCCCTTTTATCA
CGCGCAGGAGGCGACGCTGGCCATCCGCAAAGTTTTGGGCGACTTTTACC
TTCGGGACGACACGCCAGTGCCGCAGGCACTCTGGCGGGCGTACACGTGC
TGCGGCTTCATCGAGGACGACGGCGATATTGTCTTCTACAAGTCCCGGAG
CGGCCTTTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig1637:101..460+

>mRNA_P-wetherbeei_contig1637.1.1 ID=mRNA_P-wetherbeei_contig1637.1.1|Name=mRNA_P-wetherbeei_contig1637.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=360bp|location=Sequence derived from alignment at P-wetherbeei_contig1637:101..460+ (Phaeothamnion wetherbeei SAG_119_79)
AACCATAAACAGGTGCTGATCACGTTTCTGCAGCACACGGACGTGTTCCT
GCCACACTTCCGCGGCAAGGAGTGGGAGTGGTTCCGCGGCGCGCTCTGCA
CCGTCGACCGATCCTTCGGCGCCTTCATCGACCACACCATCCACCACATC
TCCGACACGCATGTGTGCCACCACCTCTTCAGCAAGATGCCCTTTTATCA
CGCGCAGGAGGCGACGCTGGCCATCCGCAAAGTTTTGGGCGACTTTTACC
TTCGGGACGACACGCCAGTGCCGCAGGCACTCTGGCGGGCGTACACGTGC
TGCGGCTTCATCGAGGACGACGGCGATATTGTCTTCTACAAGTCCCGGAG
CGGCCTTTGA
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