prot_P-wetherbeei_contig5969.2.1 (polypeptide) Phaeothamnion wetherbeei SAG_119_79

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: A0A521VLN9_9PROT (4-coumarate--CoA ligase n=2 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A521VLN9_9PROT)

HSP 1 Score: 764 bits (1972), Expect = 5.080e-274
Identity = 382/515 (74.17%), Postives = 428/515 (83.11%), Query Frame = 0

Query:    6 HASPAVPDDIGFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNYRP 520
             A PA P D G  T++ LIR HA+ +P HTALV DGR +SYAELDALMDRVAA+LQRDG+APQD+IAICAGTS++YA V+LGALRAGV VAPLAPS TA E+A MAADAGAK+ FLD  VA  LEPVAG+I A  I LDDS AG   + WLAP G+ PA   VQPQWAFNIIYSSGTTG+PKGIVQSHAMRW HVQRA  YGYS  +VT++ATPLYSNTTLVC FPTLG+GG AVLMGKFDAA YLALA +HRATHTMLVPVQYQR+MALP FD  +LSS+  KF TSAPF  +LKAD+LARWPGGL+EFYGMTEGGGTCIL AHE  AKLHTVG PA GHDIRLID+ GREV  G +GEVVG S  MMNGYH QP KTREAEW +PDG RFIR+GD+GRFD DGFLTL+DRKKDMIISGG+N+YPSDLEAVMRGH  VA+VAVVGVASE+WGETPVAF VL++GA   A AL  W NA+VN+IQRLA ++ VD+LPRSAIGKV+KR LR  Y P
Sbjct:    3 EAKPATPSDSGLRTLSELIRGHAQRQPRHTALVQDGRALSYAELDALMDRVAAALQRDGVAPQDAIAICAGTSLDYAAVFLGALRAGVAVAPLAPSGTANEIAAMAADAGAKQLFLDAPVARALEPVAGQIWAGCIMLDDSGAGEAFTAWLAPPGSSPAPVAVQPQWAFNIIYSSGTTGMPKGIVQSHAMRWGHVQRASIYGYSADTVTLLATPLYSNTTLVCFFPTLGYGGTAVLMGKFDAAAYLALAARHRATHTMLVPVQYQRIMALPEFDRHELSSFRFKFCTSAPFRPELKADILARWPGGLVEFYGMTEGGGTCILVAHEQRAKLHTVGRPAEGHDIRLIDDQGREVTPGGMGEVVGRSPIMMNGYHRQPEKTREAEWTSPDGLRFIRSGDLGRFDDDGFLTLLDRKKDMIISGGYNIYPSDLEAVMRGHADVAEVAVVGVASERWGETPVAFVVLKSGAVTSAPALLEWTNARVNRIQRLAGLQCVDSLPRSAIGKVMKRTLRHAYPP 517          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: A0A521V063_9PROT (4-coumarate--CoA ligase n=1 Tax=Betaproteobacteria bacterium TaxID=1891241 RepID=A0A521V063_9PROT)

HSP 1 Score: 754 bits (1946), Expect = 3.810e-270
Identity = 376/513 (73.29%), Postives = 425/513 (82.85%), Query Frame = 0

Query:    6 HASPAVPDDIGFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNY 518
             A+PA P +    T++ LIR HA+ +P H ALV DGR + YAELDALMDRVAA+LQRDG+APQD+I+ICAGTS++YA V+LGALRAGV VAPLAPS TA+E+A MAADAGA++ FLD   A  LEPVA ++ A+RIALDD AAG   + WLAP G  PA   VQPQWAFN+IYSSGTTG+PKGIVQSHAMRW HVQRA  YGYS  +VT++ATPLYSNTTLVC FPTLG GG AVLMGKFDAA YLALA +HRATHTMLVPVQYQR+MALP FD ++LSS+  KF TSAPF A+LKAD+LARWPGGL+EFYGMTEGGGTCIL AHE  AKLHTVG PA GHDIRLID+ G EVA GE+GEVVG S  MMNGYH QP KTREAEW +PDG RFIRTGD+GRFDADGFL L+DRKKDMIISGG+N+YPSDLE VMRGH  VADVAVVGV SE WGETPVAF VL++GA   A AL  W NA+VN+IQRLA ++ V +LPRSAIGKVLKR LRD Y
Sbjct:    3 EANPAAPTEPDLRTLSELIRGHAQRQPRHLALVQDGRALDYAELDALMDRVAAALQRDGIAPQDAISICAGTSLDYAAVFLGALRAGVAVAPLAPSGTAQEIAAMAADAGARQLFLDAPTARALEPVAERMTARRIALDDCAAGEAFTAWLAPPGTAPAPVTVQPQWAFNLIYSSGTTGVPKGIVQSHAMRWGHVQRASIYGYSADTVTLLATPLYSNTTLVCFFPTLGCGGTAVLMGKFDAAGYLALAARHRATHTMLVPVQYQRIMALPEFDRYELSSFRYKFCTSAPFRAELKADILARWPGGLVEFYGMTEGGGTCILVAHEQRAKLHTVGRPAEGHDIRLIDDQGCEVAPGEMGEVVGRSPIMMNGYHRQPEKTREAEWTSPDGLRFIRTGDLGRFDADGFLMLLDRKKDMIISGGYNIYPSDLEVVMRGHADVADVAVVGVPSEMWGETPVAFVVLKSGAVTSAPALLEWTNARVNRIQRLAGLQFVASLPRSAIGKVLKRALRDAY 515          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: A0A1F4FGI7_9PROT (4-coumarate--CoA ligase n=2 Tax=unclassified Betaproteobacteria TaxID=33809 RepID=A0A1F4FGI7_9PROT)

HSP 1 Score: 728 bits (1879), Expect = 4.560e-260
Identity = 363/503 (72.17%), Postives = 407/503 (80.91%), Query Frame = 0

Query:   16 GFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNY 518
            G  T++ LIR HA   P H AL  DGR +SY  LDALMDRVAA+LQRDGLAPQD+IAICAGTS+EYA V+LGALRAG+ VAPLAPSCT  E+A MA DAGA+  FLD +VA  L PVA ++ A+ +ALD SAAG PL  WLA +G  P    VQP WAFNIIYSSGTTG+PKGIVQSHAMR  HVQRA  YGY   +VT++ATPLYSNTTLVC FP L  GG AVLMGKFDAA YLALAE+H ATHTMLVPVQYQR+MALP F  +DL S+  KF TSAPF A LKADVLARWPGGL+EFYG+TEGGGTCIL AHEHP KLHTVG PA GHD+RLIDE GREV  G LGEVVG S  MMNGYHNQP +TREAEWY+P G+RFIR+GD+GRFDADGFL L DR+KDMII+GG+NVYPSDLE VMRGH  VADVAVVGV S++WGETPVAF VL+ GA A A AL  W N++VN+IQRLA +++   LPRSAIGKVLKRELRD Y
Sbjct:    6 GADTLSDLIRSHAARDPAHAALRQDGRELSYGALDALMDRVAAALQRDGLAPQDAIAICAGTSLEYAAVFLGALRAGIAVAPLAPSCTPSEIADMATDAGARLLFLDESVAQALAPVATRLKARCLALDGSAAGAPLRHWLAADGTAPVAIAVQPHWAFNIIYSSGTTGVPKGIVQSHAMRGGHVQRAAIYGYGRDTVTLLATPLYSNTTLVCFFPALSAGGTAVLMGKFDAAAYLALAERHHATHTMLVPVQYQRIMALPEFGRYDLQSFRYKFCTSAPFRAALKADVLARWPGGLVEFYGLTEGGGTCILVAHEHPGKLHTVGKPAQGHDVRLIDEQGREVPPGALGEVVGRSPIMMNGYHNQPERTREAEWYSPAGERFIRSGDLGRFDADGFLVLTDRRKDMIITGGYNVYPSDLETVMRGHAEVADVAVVGVPSDQWGETPVAFVVLKPGARASARALLEWTNSRVNRIQRLADLQLAANLPRSAIGKVLKRELRDAY 508          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: A0A6L4YLS2_9RHOO (Putative acyl-CoA ligase n=1 Tax=Rhodocyclaceae bacterium TaxID=1898103 RepID=A0A6L4YLS2_9RHOO)

HSP 1 Score: 722 bits (1863), Expect = 1.380e-257
Identity = 364/514 (70.82%), Postives = 409/514 (79.57%), Query Frame = 0

Query:   14 DIGFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELG---EVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNYRPGVPL 524
            ++ FGT   LIR  AR+RP H AL+ D R+VS+A LDA+MDRVAA+LQRDG+ P +++AICAGTS+EYA V+LGALRAGV VAPLAPS TA+ L  MAA+A A+  F+D A A  L  V   IAA+ I LDD AAG   S WL P GA P   D++P+W FNIIYSSGTTG PKGIVQ H MRWSHVQRA   GY P +VT+IATPLYSNTTLV VFPT+  GG  +LM KFDAAKYLALAEKHRATHTMLVPVQYQRLMA P FD +DLSS+ MK  TSAPF+A LKAD+L RWPGGL+E+YGMTEGGGTCIL AHEHP KL TVG PA GHDIRLIDE GREV  GE+G   E+VGHS  MM+GYH QP KTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAV+  H AV + AVVGVASE+WGETPVAF VL+ GA      L+ W N ++ K QRLAAVEIVD LPRSAIGK+LKRELRD + P  P+
Sbjct:    8 ELPFGTYGELIRAQARSRPNHLALIQDQRSVSFATLDAMMDRVAATLQRDGIRPTEAVAICAGTSLEYAAVFLGALRAGVAVAPLAPSSTAQSLVDMAANAEARLMFVDAATAQQLAAVRSDIAARFITLDDDAAGEAFSRWLEPAGAVPKRVDIRPEWPFNIIYSSGTTGTPKGIVQPHGMRWSHVQRARPQGYGPDAVTLIATPLYSNTTLVSVFPTVTLGGTVILMAKFDAAKYLALAEKHRATHTMLVPVQYQRLMAHPDFDRYDLSSFRMKLCTSAPFSAALKADILKRWPGGLVEYYGMTEGGGTCILAAHEHPDKLATVGAPAEGHDIRLIDEAGREVQRGEIGAIGEIVGHSPAMMSGYHRQPDKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVLIRHPAVNEAAVVGVASERWGETPVAFVVLKVGAGETPTQLQNWTNERLGKTQRLAAVEIVDALPRSAIGKILKRELRDLFHPAAPI 521          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: A0A1F4CK77_9PROT (4-coumarate--CoA ligase n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_12_FULL_62_13b TaxID=1797496 RepID=A0A1F4CK77_9PROT)

HSP 1 Score: 720 bits (1859), Expect = 5.810e-257
Identity = 356/506 (70.36%), Postives = 406/506 (80.24%), Query Frame = 0

Query:   14 DIGFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNYR 519
            D  FG +  L+R HA  RP H ALV D R + YA LDALMDRVAASLQ +GL PQD+IAICA TS+EYA V+LGALRAG+ VAPLAP+ TAE LAGM ADAGAK  F+D A ++ L PV  +IAAQ IALD SA       WLA EGARP    ++P+WAFNIIYSSGTTG PKGIVQ H+MRW H+QRA  Y Y P +VT++ATPLYSNTTLV  FPTLG GG AVLMGKFDAA YLALAEKHRATHTMLVPVQYQR+MAL  F+ FDLS++  K  TSAPF A LKAD+L RWPG L EFYG+TEGGGTC+L AHEHP KLHTVG PAPGHDIRLIDE GREVA GE GE+VG S  MM+GYHNQPA T +AEW+   G+RF+RTGD+GRFDADGFLTL+DRKKDMIISGGFN+YPSDLE V+R H  VA+ AVVGV+S +WGETPVAF VL+TG+ A A  L AWAN ++ K+QRLAAVE+V+ LPRSAIGKV+KRELR+ YR
Sbjct:   10 DQPFGAIPDLVRAHAAQRPRHAALVHDRRVLDYAALDALMDRVAASLQHEGLKPQDTIAICAATSLEYAAVFLGALRAGIAVAPLAPTSTAESLAGMVADAGAKLLFVDAAASDVLAPVRSRIAAQCIALDGSAGADAFDAWLAAEGARPEAVSIRPEWAFNIIYSSGTTGAPKGIVQPHSMRWGHLQRAAPYDYGPDAVTLLATPLYSNTTLVSFFPTLGMGGTAVLMGKFDAAVYLALAEKHRATHTMLVPVQYQRIMALADFNRFDLSAFRFKLCTSAPFAASLKADILKRWPGALCEFYGLTEGGGTCLLAAHEHPDKLHTVGRPAPGHDIRLIDEQGREVAAGEAGEIVGRSPVMMSGYHNQPAMTAQAEWFDAQGRRFLRTGDIGRFDADGFLTLLDRKKDMIISGGFNIYPSDLELVLREHPGVAEAAVVGVSSARWGETPVAFVVLKTGSVATAAELLAWANGRLGKMQRLAAVELVEGLPRSAIGKVMKRELREAYR 515          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: UPI00124D8357 (acyl--CoA ligase n=2 Tax=Noviherbaspirillum TaxID=1344552 RepID=UPI00124D8357)

HSP 1 Score: 717 bits (1850), Expect = 1.310e-255
Identity = 358/519 (68.98%), Postives = 411/519 (79.19%), Query Frame = 0

Query:    1 MNTSTHASPAVPDDIGFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNYR 519
            M+   H    + D   F T+  LI  HA+ARP H ALV DG+T+SYAELD LMDRVAA+LQRDG+ P+D+IAICAGTS+ YA V+LG+LRAGV VAPLAPS + E L GMA DA AK  F+D AVA  L PV G++    IALD S AG  +  WLAP G+RP   DVQP WAFNIIYSSGTTG PKGIVQ H+MRW+HVQR    GY  ++VT+I+TPLYSNTTLVC FPT+  GG  V+M KFDA  YL+LA++HR THTMLVPVQYQR+MA   F  FDLSS+ MKF TSAPF+A LKAD+L RWPGGLIE+YGMTEGGGTC+L AHEHP KLHTVG PAPGHDIRLID+ GREV  GELGEVVGHS+ MM GYH QP KT EAEW+ P GKRFIRTGD+GRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAV+  H  V + AVVG+ S KWGETPVAF V++TG+A +A ALRAWAN ++ K QRLAAVE+ + LPRSAIGKVLKRELRD ++
Sbjct:    1 MSDPLHIDTILEDS--FHTLPYLIHLHAKARPDHPALVQDGKTLSYAELDQLMDRVAAALQRDGIQPRDAIAICAGTSLFYAAVFLGSLRAGVAVAPLAPSSSPESLVGMAGDADAKLLFVDHAVAQQLVPVRGQLFMPVIALDGSDAGRAIEDWLAPAGSRPQAVDVQPDWAFNIIYSSGTTGAPKGIVQPHSMRWTHVQRGRINGYDENAVTLISTPLYSNTTLVCFFPTVSLGGTVVMMAKFDAGAYLSLAQQHRVTHTMLVPVQYQRIMAREDFGQFDLSSFRMKFCTSAPFSAALKADILKRWPGGLIEYYGMTEGGGTCVLAAHEHPDKLHTVGQPAPGHDIRLIDDAGREVGPGELGEVVGHSSAMMAGYHKQPQKTAEAEWFDPSGKRFIRTGDIGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVLHQHPDVVEAAVVGMPSVKWGETPVAFVVVKTGSALDAPALRAWANERLGKTQRLAAVELAEVLPRSAIGKVLKRELRDGFK 517          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: W0SGF0_9PROT (4-coumarate--CoA ligase n=1 Tax=Sulfuritalea hydrogenivorans sk43H TaxID=1223802 RepID=W0SGF0_9PROT)

HSP 1 Score: 716 bits (1849), Expect = 2.980e-255
Identity = 366/524 (69.85%), Postives = 407/524 (77.67%), Query Frame = 0

Query:   17 FGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAA----------------EAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNYRPGVPL 524
            F T A LIR+ AR RPGH AL+ D R+VS+A LDA+MDRVAA+LQRDG+ P +++AICAG S+EYA V+LGALRAGV VAPLAPS T + LA MAA+A A+  F+D A A  L  V   I A+ IALDDS  G   S WLAP G+ P    V+P+W FNIIYSSGTTG PKGIVQ H MRWSHVQRA   GY P SVT+IATPLYSNTTLV  FPT+  GG  VLM KFDAAKYLALAEKHRATHTMLVPVQYQRLMA P FD  DLSS+ MK  TSAPF A LKAD+L RWPGGL+E+YGMTEGGGTCIL AHEHP KL TVG PA GHD+RLIDE G EVA GE+GEVVGHS  MM+GYH QP KTREAEWYAPDGKRFIRTGDVGRFD DGFLTLMDRKKDM+ISGGFNVYPSDLEAV+  H AV++ AVVGVASE+WGETPVAF  LR GA +                  + LR WANA++ K QRLAAVEIVD LPRSAIGK+LKRELRD++ P  PL
Sbjct:   11 FATYAELIREQARTRPGHPALIQDRRSVSFATLDAMMDRVAATLQRDGIRPTEAVAICAGASLEYAAVFLGALRAGVAVAPLAPSSTPQSLADMAANAEAELMFVDAATARELAAVRADIKARFIALDDSTGGEAFSRWLAPAGSVPTPVQVRPEWPFNIIYSSGTTGTPKGIVQPHGMRWSHVQRARRQGYGPGSVTVIATPLYSNTTLVSFFPTVTLGGTVVLMAKFDAAKYLALAEKHRATHTMLVPVQYQRLMAHPDFDRHDLSSFRMKLCTSAPFPAALKADILKRWPGGLVEYYGMTEGGGTCILAAHEHPDKLATVGTPAEGHDMRLIDEAGHEVARGEIGEVVGHSPAMMSGYHRQPDKTREAEWYAPDGKRFIRTGDVGRFDEDGFLTLMDRKKDMVISGGFNVYPSDLEAVLIQHPAVSEAAVVGVASERWGETPVAFVTLRVGAGSLCVPGNPLRGQATAKCTTDELREWANAQLGKTQRLAAVEIVDALPRSAIGKILKRELRDSFAPAEPL 534          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: A0A1G3H4H2_9RHOO (4-coumarate--CoA ligase n=1 Tax=Rhodocyclales bacterium GWA2_65_20 TaxID=1802017 RepID=A0A1G3H4H2_9RHOO)

HSP 1 Score: 704 bits (1816), Expect = 1.930e-250
Identity = 355/518 (68.53%), Postives = 406/518 (78.38%), Query Frame = 0

Query:    6 HASPAVPDDIGFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELG---EVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNYRP 520
            H SP +     FGT   LIR+ AR+ P H AL+ D RTVS+A LDA+MDRVAA+LQR+G+ P +++AICAGTS+EYA V+LGALRAGV VAPLAPS TA+ +A MAA+A A+  F+D   A  L PV   IAA+ +ALDD+A    L  WLAP G  P   D++P+W FNIIYSSGTTG PKGIVQ H MRW+H+QRA   GY P +VT+IATPLYSNTTLV  FPT+  GG  VLM KFDAA+YLALAEK+ ATHTMLVPVQYQRLMA P FD FDLS + MK  TSAPF A LKAD+L RWPGGL+E+YGMTEGGGTCIL AHE P KL TVG PA GHDIRLIDE GREV  GE+G   EVVGHS  MM+GYH QP +TREAEW+APDGKRFIRTGDVGRFDADGFLTLMDRKKDM+ISGGFNVYPSDLEAV+  H AVA+ AVVGVASE+WGETP+AF  LR GA   A  +  W N ++ K QRLAAVEIVD LPRSAIGK+LKRELR+N+ P
Sbjct:    2 HPSPLIEQP--FGTYGELIRERARSSPDHPALIQDRRTVSFATLDAMMDRVAATLQREGIRPTEAVAICAGTSLEYAAVFLGALRAGVAVAPLAPSSTAQSIADMAANAEARLIFVDAITAEQLAPVRAGIAARFVALDDAAGAQALRQWLAPAGTVPDAVDIRPEWPFNIIYSSGTTGTPKGIVQPHGMRWAHLQRAQPQGYGPDAVTLIATPLYSNTTLVSFFPTVTLGGTVVLMAKFDAARYLALAEKYHATHTMLVPVQYQRLMAHPDFDRFDLSGFRMKLCTSAPFAADLKADILKRWPGGLVEYYGMTEGGGTCILAAHERPDKLATVGTPAKGHDIRLIDEAGREVPRGEIGAIGEVVGHSPAMMSGYHRQPEQTREAEWHAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMVISGGFNVYPSDLEAVLIRHPAVAEAAVVGVASERWGETPIAFVTLRVGAGDTAMQILNWTNHQLGKTQRLAAVEIVDALPRSAIGKILKRELRENFHP 517          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: UPI001BA84F7C (acyl--CoA ligase n=1 Tax=Caulobacter sp. S6 TaxID=2823693 RepID=UPI001BA84F7C)

HSP 1 Score: 703 bits (1815), Expect = 2.460e-250
Identity = 352/502 (70.12%), Postives = 396/502 (78.88%), Query Frame = 0

Query:   17 FGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNY 518
            FGT+  LI  HA  RP H AL+   R V+Y  LDALMDRVAA LQRDG  P  ++A CA TSIEY   +LGALRAGV +APLAPS T E LAGM AD+GA   FLD  V+  LE V+G+I+A+ +ALD S AG     WL P G++P   ++QP W FNIIYSSGTTG PKGIVQSHAMRW HV R    GY P +VTMI+TPLYSNTTLVC+ PTL  GG AVLM KFD  ++L LAE+HRATH MLVPVQY+RLM    FD FDLSS+ MKF+TSAPF+A LKADVL RWPGGLIE++GMTEGGGT IL AHEHP KLHTVG PAPGHDIRLIDE+GREV  GE+GEVVGHS  MMNGYHNQP KT +AEW    GKRFIRTGDVGRFDA+GFLTLMDRKKDMIISGGFN+YPSDLEAV+ GHEAVA+ AVVG  S +WGETP+A  VLR GAA EAEALRAWAN ++ K QRLAA+EI+ +LPRSAIGKVLKRELRD Y
Sbjct:   11 FGTLPALIAAHAAERPDHVALIQGERKVTYGGLDALMDRVAAGLQRDGAEPGGAVAACALTSIEYVAAFLGALRAGVAMAPLAPSSTPESLAGMIADSGASLLFLDQGVSEQLEGVSGQISARPVALDGSDAGEAFEAWLPPAGSKPEPVEIQPDWPFNIIYSSGTTGAPKGIVQSHAMRWQHVGRGPAGGYGPDAVTMISTPLYSNTTLVCLIPTLAGGGTAVLMEKFDVVRFLELAERHRATHAMLVPVQYRRLMEHADFDRFDLSSFRMKFATSAPFSAALKADVLKRWPGGLIEYFGMTEGGGTFILAAHEHPDKLHTVGRPAPGHDIRLIDEEGREVPVGEIGEVVGHSPAMMNGYHNQPGKTADAEWRDASGKRFIRTGDVGRFDAEGFLTLMDRKKDMIISGGFNIYPSDLEAVVAGHEAVAEAAVVGAPSNRWGETPIALVVLRDGAAIEAEALRAWANERLGKTQRLAALEILPSLPRSAIGKVLKRELRDQY 512          

BLAST of mRNA_P-wetherbeei_contig5969.2.1 vs. uniprot
Match: A0A3A3GCB0_9BURK (4-coumarate--CoA ligase n=2 Tax=Noviherbaspirillum saxi TaxID=2320863 RepID=A0A3A3GCB0_9BURK)

HSP 1 Score: 703 bits (1814), Expect = 3.890e-250
Identity = 352/518 (67.95%), Postives = 406/518 (78.38%), Query Frame = 0

Query:    1 MNTSTHASPAVPDDIGFGTVAGLIRDHARARPGHTALVMDGRTVSYAELDALMDRVAASLQRDGLAPQDSIAICAGTSIEYATVYLGALRAGVVVAPLAPSCTAEELAGMAADAGAKRFFLDTAVANTLEPVAGKIAAQRIALDDSAAGTPLSGWLAPEGARPAVADVQPQWAFNIIYSSGTTGLPKGIVQSHAMRWSHVQRAVGYGYSPSSVTMIATPLYSNTTLVCVFPTLGHGGAAVLMGKFDAAKYLALAEKHRATHTMLVPVQYQRLMALPGFDAFDLSSYHMKFSTSAPFNAKLKADVLARWPGGLIEFYGMTEGGGTCILHAHEHPAKLHTVGPPAPGHDIRLIDEDGREVAEGELGEVVGHSAGMMNGYHNQPAKTREAEWYAPDGKRFIRTGDVGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVMRGHEAVADVAVVGVASEKWGETPVAFAVLRTGAAAEAEALRAWANAKVNKIQRLAAVEIVDTLPRSAIGKVLKRELRDNY 518
            M   ++    + D+  F  +  L++ HA A P   ALV DG T++YAELDALMDR+AASLQRDG+ P+D+IAICAGTSI YA V+LG+LRAGV VAPLAPS +A+ L  MA DA A+  FLD AVA  L PV   +AA  IALD S AG     WLAP G++P   D+ P+W FNIIYSSGTTG PKGIVQ ++MRW+HVQR    GY P +VT+I+TPLYSNTTLVC FPT+  GG  V+M KFDAAKYL LA++HR THTMLVPVQYQR+MA   FD +DLSS+ MKF TSAPF+A LKAD+L RWPGGLIE+YGMTEGGGTC+L AHEHP KLHTVG PAPGHDIRLIDE G+EV   ELGEVVGHS  MM GYHNQP KT EAEW  P GKRFIRTGD+GRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAV+  H  V + AVVG+ S +WGETPVAFAV+R GA+ +A AL +WAN ++ K QRLAAVEI D LPRSAIGKVLKRELRD++
Sbjct:    1 MTAQSNIDTVLEDN--FHPLPYLVQLHAAAMPTKPALVQDGTTLTYAELDALMDRIAASLQRDGIGPRDTIAICAGTSIPYAAVFLGSLRAGVAVAPLAPSSSADSLVTMAKDADARLLFLDQAVAEQLAPVRAILAAPVIALDGSKAGQAFENWLAPPGSKPQPVDIAPEWPFNIIYSSGTTGAPKGIVQPYSMRWTHVQRGRSNGYDPEAVTLISTPLYSNTTLVCFFPTVSLGGTVVMMAKFDAAKYLQLAQQHRVTHTMLVPVQYQRIMARADFDQYDLSSFRMKFCTSAPFSAALKADILRRWPGGLIEYYGMTEGGGTCMLVAHEHPDKLHTVGQPAPGHDIRLIDESGKEVGADELGEVVGHSPAMMAGYHNQPQKTAEAEWVDPSGKRFIRTGDIGRFDADGFLTLMDRKKDMIISGGFNVYPSDLEAVLHQHPDVVEAAVVGMPSTRWGETPVAFAVIRPGASLDAPALLSWANERLGKTQRLAAVEITDVLPRSAIGKVLKRELRDSF 516