mRNA_P-wetherbeei_contig6405.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig6405.1.1
Unique NamemRNA_P-wetherbeei_contig6405.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: A0A6H5K9Q0_9PHAE (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K9Q0_9PHAE)

HSP 1 Score: 144 bits (364), Expect = 5.810e-39
Identity = 70/101 (69.31%), Postives = 82/101 (81.19%), Query Frame = 1 
Query:    7 AAGWPEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            A G PEPGPA+LVEERDACGVGF+A       HKV+ELGL ALGCMEHRGACLAD +SGDG+GIMT++PW L+EREY+  GV+  +LPPR  RA+GM FLP
Sbjct:    6 AKGTPEPGPAWLVEERDACGVGFIANPKP--EHKVVELGLAALGCMEHRGACLADNVSGDGSGIMTSVPWALLEREYSDMGVETDSLPPRDQRAVGMLFLP 104
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: D8LB71_ECTSI (Glutamate synthase (Ferredoxin-dependent) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LB71_ECTSI)

HSP 1 Score: 143 bits (361), Expect = 1.280e-37
Identity = 69/99 (69.70%), Postives = 81/99 (81.82%), Query Frame = 1 
Query:   13 GWPEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            G PEPGPA+LVEERDACGVGF+A       HKV+ELGL ALGCMEHRGACLAD +SGDG+GIMT++PW L+EREY+  GV+  +LPPR  RA+GM FLP
Sbjct:   55 GTPEPGPAWLVEERDACGVGFIANPKP--EHKVVELGLAALGCMEHRGACLADNVSGDGSGIMTSVPWALLEREYSDMGVETDSLPPRDQRAVGMLFLP 151
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: A0A835YTV2_9STRA (Glutamate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YTV2_9STRA)

HSP 1 Score: 127 bits (320), Expect = 4.340e-32
Identity = 64/102 (62.75%), Postives = 75/102 (73.53%), Query Frame = 1 
Query:    4 RAAGWPEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            R+ G PEPGPA+LVEERDACGVGF+A   A + HKV+  GL AL CMEHRGACL+D +SGDG+GIMTAIPW+L+ER  A   +      PR  R LGM FLP
Sbjct:   73 RSMGVPEPGPAWLVEERDACGVGFIANIDATSTHKVVSSGLTALSCMEHRGACLSDNVSGDGSGIMTAIPWDLLERSGAFGTLPA----PRDQRGLGMVFLP 170
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: A0A7S3U6R9_9SPIT (Hypothetical protein (Fragment) n=1 Tax=Strombidinopsis acuminata TaxID=141414 RepID=A0A7S3U6R9_9SPIT)

HSP 1 Score: 108 bits (271), Expect = 8.500e-29
Identity = 56/101 (55.45%), Postives = 64/101 (63.37%), Query Frame = 1 
Query:    7 AAGWPEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            A G PEPGP  LV ERDACGVGF+A      NH ++E  L ALGCMEHRG C  D +SGDGAG+MTA+PW+L E E A AG       P     + M FLP
Sbjct:    3 ALGTPEPGPMPLVGERDACGVGFIADTKRRNNHDIVERALYALGCMEHRGGCGGDRVSGDGAGVMTAVPWKLFEEEGALAG------KPAESMGVAMAFLP 97
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: A0A6P0N7W9_9CYAN (Glutamate synthase subunit alpha (Fragment) n=1 Tax=Moorena sp. SIO3C2 TaxID=2607842 RepID=A0A6P0N7W9_9CYAN)

HSP 1 Score: 111 bits (278), Expect = 2.030e-28
Identity = 54/97 (55.67%), Postives = 68/97 (70.10%), Query Frame = 1 
Query:   19 PEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            P PG  +LVEERDACGVGF+A    +A HK+IE  L ALGC+EHRG C AD  SGDGAG++TAIPW+L+++ +   G+D    PP     +GM FLP
Sbjct:   21 PYPGQRWLVEERDACGVGFIASGQGIATHKLIEQALVALGCLEHRGGCSADQDSGDGAGLLTAIPWKLLQQWFTEQGIDQ---PPTEQLGVGMVFLP 114
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: UPI00188112BD (glutamate synthase-related protein n=1 Tax=unclassified Coleofasciculus TaxID=2692782 RepID=UPI00188112BD)

HSP 1 Score: 113 bits (283), Expect = 4.270e-27
Identity = 57/97 (58.76%), Postives = 69/97 (71.13%), Query Frame = 1 
Query:   19 PEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            P PG  +LVEERDACGVGF+A  +  A+HK+IE  L ALGC+EHRGAC AD  SGDGAG+MTAIPW+L E  +A  G+    +PP     +GM FLP
Sbjct:   21 PYPGQRWLVEERDACGVGFIASTAGTASHKLIEQALIALGCLEHRGACSADQDSGDGAGLMTAIPWKLFEPWFAEQGIT---MPPTEQLGVGMVFLP 114
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: F4XKW1_9CYAN (Glutamate synthase, ferredoxin n=24 Tax=Moorena TaxID=1155738 RepID=F4XKW1_9CYAN)

HSP 1 Score: 110 bits (276), Expect = 3.760e-26
Identity = 54/97 (55.67%), Postives = 68/97 (70.10%), Query Frame = 1 
Query:   19 PEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            P PG  +LVEERDACGVGF+A    +A+HK+IE  L ALGC+EHRG C AD  SGDGAG++TAIPW+L+++     G+D    PP     +GM FLP
Sbjct:   21 PYPGQRWLVEERDACGVGFIASGQGIASHKLIEQALVALGCLEHRGGCSADQDSGDGAGLLTAIPWKLLQKWLTEQGIDQ---PPTEQLGVGMVFLP 114
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: A0A3C0N0L3_9CYAN (Glutamate synthase subunit alpha n=1 Tax=Cyanobacteria bacterium UBA8543 TaxID=2055766 RepID=A0A3C0N0L3_9CYAN)

HSP 1 Score: 108 bits (269), Expect = 3.310e-25
Identity = 54/97 (55.67%), Postives = 67/97 (69.07%), Query Frame = 1 
Query:   19 PEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            P PG  +LVEERDACGVGF+A  +  A H++IE  L ALGC+EHRG C AD  SGDGAG+MTAIPW L ++ +A A +    +PP     +GM FLP
Sbjct:   20 PFPGQRWLVEERDACGVGFIASQNGTATHELIEQALSALGCLEHRGGCSADQDSGDGAGLMTAIPWNLFKQWFAQAEIS---MPPTDQLGVGMFFLP 113
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: A0A6B3MCP7_9CYAN (Glutamate synthase large subunit n=4 Tax=Cyanobacteria TaxID=1117 RepID=A0A6B3MCP7_9CYAN)

HSP 1 Score: 108 bits (269), Expect = 3.310e-25
Identity = 53/95 (55.79%), Postives = 66/95 (69.47%), Query Frame = 1 
Query:   25 PGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            PG  +LVEERDACGVGF+     L  H++IE  L ALGC+EHRG C AD  SGDGAG+M+AIPW+L+E  +A  G++   LPP     +GM FLP
Sbjct:   23 PGQRWLVEERDACGVGFIVSEPGLITHELIEQALSALGCLEHRGGCSADQDSGDGAGLMSAIPWKLLESWFAQQGIE---LPPTEQLGVGMVFLP 114
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BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Match: A0A1C0UUE6_9CYAN (Glutamate synthase subunit alpha n=7 Tax=Limnothrix TaxID=132605 RepID=A0A1C0UUE6_9CYAN)

HSP 1 Score: 108 bits (269), Expect = 3.310e-25
Identity = 56/97 (57.73%), Postives = 66/97 (68.04%), Query Frame = 1 
Query:   19 PEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEHRGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMCFLP 309
            PE G  +L EERDACGVGF+AQ  A A+H ++E  L ALGCMEHRG C AD  SGDGAG+MTAIPW L+E+  A  G  +     R   A+GM FLP
Sbjct:   20 PESGLRYLTEERDACGVGFIAQRQAQASHDLVEKALIALGCMEHRGGCSADRDSGDGAGVMTAIPWTLIEQWAATEGKTI----DRDRTAVGMLFLP 112
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig6405.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5K9Q0_9PHAE5.810e-3969.31Glutamine amidotransferase type-2 domain-containin... [more]
D8LB71_ECTSI1.280e-3769.70Glutamate synthase (Ferredoxin-dependent) n=1 Tax=... [more]
A0A835YTV2_9STRA4.340e-3262.75Glutamate synthase n=1 Tax=Tribonema minus TaxID=3... [more]
A0A7S3U6R9_9SPIT8.500e-2955.45Hypothetical protein (Fragment) n=1 Tax=Strombidin... [more]
A0A6P0N7W9_9CYAN2.030e-2855.67Glutamate synthase subunit alpha (Fragment) n=1 Ta... [more]
UPI00188112BD4.270e-2758.76glutamate synthase-related protein n=1 Tax=unclass... [more]
F4XKW1_9CYAN3.760e-2655.67Glutamate synthase, ferredoxin n=24 Tax=Moorena Ta... [more]
A0A3C0N0L3_9CYAN3.310e-2555.67Glutamate synthase subunit alpha n=1 Tax=Cyanobact... [more]
A0A6B3MCP7_9CYAN3.310e-2555.79Glutamate synthase large subunit n=4 Tax=Cyanobact... [more]
A0A1C0UUE6_9CYAN3.310e-2557.73Glutamate synthase subunit alpha n=7 Tax=Limnothri... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig6405contigP-wetherbeei_contig6405:716..1272 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D8LB71
Preferred nameGLU
PFAMsFer4_20,GATase_2,GXGXG,Glu_syn_central,Glu_synthase,Pyr_redox_2
Model size309
Max annot lvl2759|Eukaryota
KEGG rclassRC00006,RC00010,RC02799
KEGG koko:K00264,ko:K00284
KEGG ReactionR00021,R00093,R00114,R00248,R10086
KEGG Pathwayko00250,ko00630,ko00910,ko01100,ko01110,ko01120,ko01130,ko01230,map00250,map00630,map00910,map01100,map01110,map01120,map01130,map01230
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005759,GO:0006082,GO:0006520,GO:0006536,GO:0006537,GO:0006541,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009064,GO:0009084,GO:0009314,GO:0009416,GO:0009507,GO:0009526,GO:0009532,GO:0009536,GO:0009570,GO:0009628,GO:0009743,GO:0009744,GO:0009853,GO:0009941,GO:0009987,GO:0010033,GO:0015930,GO:0016020,GO:0016041,GO:0016053,GO:0016491,GO:0016638,GO:0016643,GO:0019222,GO:0019676,GO:0019740,GO:0019752,GO:0019899,GO:0031323,GO:0031967,GO:0031974,GO:0031975,GO:0034285,GO:0042221,GO:0043085,GO:0043094,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0043648,GO:0043650,GO:0044093,GO:0044237,GO:0044238,GO:0044249,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044429,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0046394,GO:0048046,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051338,GO:0051347,GO:0055114,GO:0065007,GO:0065009,GO:0070013,GO:0071704,GO:0080114,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901700
Exons2
Evalue2.1e-38
EggNOG OGsCOG0069@1|root,KOG0399@2759|Eukaryota
EC1.4.1.13,1.4.1.14,1.4.7.1
DescriptionL-glutamate biosynthetic process
Cds size309
COG categoryE
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186910.7340705-CDS-P-wetherbeei_contig6405:715..8911683186910.7340705-CDS-P-wetherbeei_contig6405:715..891Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig6405 716..891 +
1683186910.7522335-CDS-P-wetherbeei_contig6405:1139..12721683186910.7522335-CDS-P-wetherbeei_contig6405:1139..1272Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig6405 1140..1272 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig6405.1.1prot_P-wetherbeei_contig6405.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig6405 716..1272 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig6405.1.1

>prot_P-wetherbeei_contig6405.1.1 ID=prot_P-wetherbeei_contig6405.1.1|Name=mRNA_P-wetherbeei_contig6405.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=103bp
VRAAGWPEPGPAFLVEERDACGVGFVAQNSALANHKVIELGLRALGCMEH
RGACLADGISGDGAGIMTAIPWELVEREYAAAGVDVAALPPRAHRALGMC
FLP
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mRNA from alignment at P-wetherbeei_contig6405:716..1272+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig6405.1.1 ID=mRNA_P-wetherbeei_contig6405.1.1|Name=mRNA_P-wetherbeei_contig6405.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=557bp|location=Sequence derived from alignment at P-wetherbeei_contig6405:716..1272+ (Phaeothamnion wetherbeei SAG_119_79)
GTCCGGGCAGCGGGATGGCCGGAACCCGGCCCCGCGTTCCTGGTCGAGGA
GAGGGACGCCTGCGGTGTCGGATTCGTCGCTCAAAACAGCGCCCTGGCGA
ACCACAAGGTGATCGAGCTCGGGCTGCGGGCGCTGGGCTGCATGGAGCAC
CGCGGTGCCTGCCTCGCGGACGGCATGTGAGTTTCCGTTGGCGAGGGGGT
TGTTTTGTTTTGCTGTTTATTATATGAGAACCTATGTCTTAAATTGACGT
TGACGTTGATGGAAATTGGTTTCGTTTAAGCTTAAATTGCTCAATTGCGG
ATGTTTGCGCGTTTTGCCAGGACTGCCGGAACTGCCGAAATGAAATTCGT
AGCTCCGCGTGTCAAAAAGTGCAACTTCTAACCAAAATTCTTCCCTGCAA
TTTCTTTTTCTTTTTCGTTACCAGCTCGGGCGACGGCGCCGGCATCATGA
CGGCCATCCCCTGGGAGCTGGTCGAGCGCGAGTATGCCGCCGCCGGCGTC
GACGTCGCCGCGCTGCCGCCCCGCGCACACCGCGCGCTCGGTATGTGCTT
CCTGCCA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig6405:716..1272+

>mRNA_P-wetherbeei_contig6405.1.1 ID=mRNA_P-wetherbeei_contig6405.1.1|Name=mRNA_P-wetherbeei_contig6405.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=309bp|location=Sequence derived from alignment at P-wetherbeei_contig6405:716..1272+ (Phaeothamnion wetherbeei SAG_119_79)
GTCCGGGCAGCGGGATGGCCGGAACCCGGCCCCGCGTTCCTGGTCGAGGA
GAGGGACGCCTGCGGTGTCGGATTCGTCGCTCAAAACAGCGCCCTGGCGA
ACCACAAGGTGATCGAGCTCGGGCTGCGGGCGCTGGGCTGCATGGAGCAC
CGCGGTGCCTGCCTCGCGGACGGCATCTCGGGCGACGGCGCCGGCATCAT
GACGGCCATCCCCTGGGAGCTGGTCGAGCGCGAGTATGCCGCCGCCGGCG
TCGACGTCGCCGCGCTGCCGCCCCGCGCACACCGCGCGCTCGGTATGTGC
TTCCTGCCA
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