mRNA_P-wetherbeei_contig6028.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig6028.1.1
Unique NamemRNA_P-wetherbeei_contig6028.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: A0A2D4BN96_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BN96_PYTIN)

HSP 1 Score: 121 bits (304), Expect = 5.970e-29
Identity = 72/165 (43.64%), Postives = 102/165 (61.82%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNGPGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAF----RRELATLTGVAELPAAQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            QAF E D + DGRL  EEF+ WY      G +  DG+    F++ E + L +L +    +VF  +A  +DE GF++++AF    R  +A+  G   +   +  R   ++ RLF + D + +G VDFSE++SGL+V CGGT DEKVRAAF+LYD NGDGFI +DEM
Sbjct:  179 QAFAEADQDHDGRLTFEEFQEWYQQPGGIGEVAKDGEQL--FSLDEARRLTKLGDFSPLEVFEALADCADEQGFVSKEAFDSCFRSFIASKRG--GIRGDEYERISAVLNRLFELFDVDKNGVVDFSEISSGLSVFCGGTSDEKVRAAFSLYDYNGDGFISLDEM 339
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: A0A8K1FFW6_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FFW6_PYTOL)

HSP 1 Score: 121 bits (303), Expect = 8.630e-29
Identity = 69/165 (41.82%), Postives = 101/165 (61.21%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNGPGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIA----EDAFRRELATLTGVAELPAAQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            QAF E D++ DGRL  EEF  WY  +   G +  DG+    F++ E + L  L      +VF  +A  +D  G+++    +D FR+ +A+  G   + A +  R   I+ RLF + D + +G VDFSE++SGL+V CGG+ DEKVRAAF+LYD NGDG+I ++EM
Sbjct: 1630 QAFAEADLDHDGRLTFEEFEAWYQQSGGIGEVAKDGEQL--FSLEEARRLTNLGAISPMEVFEALAECADGQGYVSREAFDDCFRKFIASRRG-RSIQAGEYERISVILNRLFDLFDVDKNGVVDFSEISSGLSVFCGGSSDEKVRAAFSLYDYNGDGYISLEEM 1791
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: K3WY98_GLOUD (Uncharacterized protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WY98_GLOUD)

HSP 1 Score: 119 bits (299), Expect = 2.930e-28
Identity = 69/163 (42.33%), Postives = 98/163 (60.12%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNGPGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAFRRELATLTGVAELPA--AQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            + F + D+N DGRLN +EF+ WY           +    P   + E++ L  L    V +VF + A  +DE G +  D+F +    +  +A  P   A++ RA+ I+ RLF V D +G+G++DFSELASGL+VLC G RD KV+AAF LYD NGDG+I +DEM
Sbjct:  530 EIFADADLNHDGRLNFDEFKKWYTLPE---QSSFNAIVAP-LDLNEVRRLTNLGTLDVVEVFERFAEHADEDGLLQRDSFDKCFLEIIEMASHPRTDAEKLRAKVIVDRLFDVFDQDGNGQIDFSELASGLSVLCKGARDAKVKAAFRLYDFNGDGYISLDEM 688
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: A0A5D6YAD8_9STRA (Uncharacterized protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YAD8_9STRA)

HSP 1 Score: 117 bits (293), Expect = 1.910e-27
Identity = 72/169 (42.60%), Postives = 96/169 (56.80%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWY------NANRCGGSLGGDGDNGPGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAFRRELATLTGVAELPA--AQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            + F E D+N DGRLN +EF+ WY      + N     L           + E++ L  L    V +VF + A  +D  G +  +AF +    +   A  P   A++ RA+ I  RLF V D +GDG++DFSELASGL+VLC G RD KV+AAF LYD NGDGFI +DEM
Sbjct: 1235 EIFAEADLNHDGRLNFDEFKKWYALPEQSSFNAIVAPL----------DLDEVRRLTNLGTLDVVEVFERFAEYADVGGLLQREAFDQCFLEIIETASHPRSDAEKLRAKVIADRLFDVFDQDGDGQIDFSELASGLSVLCKGARDAKVKAAFRLYDFNGDGFISLDEM 1393
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: A0A6T5TER1_9STRA (Hypothetical protein n=1 Tax=Triparma pacifica TaxID=91992 RepID=A0A6T5TER1_9STRA)

HSP 1 Score: 115 bits (288), Expect = 8.660e-27
Identity = 69/175 (39.43%), Postives = 98/175 (56.00%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGG-----------DGDNGPGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAFRRELATLTG--VAELPAAQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEML 489
            +AF E D+N DGRL+ +EF+ WY  N+ GG                G   P  T+ E+Q +  L + HV+DVF   A  +DE G++ +++F    ATL    +  L A    R   ++  LF  +D NGDGR+D+ ELASG++VLCGG RD+KVRAAF+L          +DEM+
Sbjct:  489 EAFREADLNKDGRLSFDEFKKWYTNNQMGGGQDPTEYDPEVYDLESGGTHP-VTMEEVQKITGLEDQHVEDVFELFASHADEEGYLNKESFFDCFATLIRGRIEALDAKDYKRVLFVVESLFQAVDLNGDGRIDYCELASGISVLCGGGRDDKVRAAFSLXXXXXXXXXSLDEMV 662
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: D0N1M0_PHYIT (Uncharacterized protein n=1 Tax=Phytophthora infestans (strain T30-4) TaxID=403677 RepID=D0N1M0_PHYIT)

HSP 1 Score: 115 bits (288), Expect = 8.940e-27
Identity = 67/166 (40.36%), Postives = 100/166 (60.24%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNGPG-FTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAF----RRELATLTGVAELPAAQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            QAF E D + DG+L  EEF+ WY      G +G    NG   F++ E + L  L      +VF  +A  +DE G+++ +AF    R+ ++T  G+    + +  R  T++ RLF + D + +G VDFSE++SGL+V CGG+ +EK+RAAF LYD N DGFI ++EM
Sbjct: 1482 QAFAEADQDHDGQLTLEEFQQWYQQP---GGIGEVAKNGEQLFSLAEARRLTNLQSFSPMEVFEALAECADEQGYVSREAFDKCFRKIISTNQGI---KSEEYQRINTVLNRLFELFDVDKNGLVDFSEISSGLSVFCGGSSEEKIRAAFALYDFNADGFISMEEM 1641
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: A0A024THC6_9STRA (EF-hand domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024THC6_9STRA)

HSP 1 Score: 109 bits (272), Expect = 1.370e-26
Identity = 66/162 (40.74%), Postives = 97/162 (59.88%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNGPGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAFRRELATLTGVAELPAAQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEML 489
            QAF ++++N DGR+  +EF+ WY++    G+      N   FT+   + L +L     D VF  +A  +D +G + +DAF         V  L     AR+   I RLF + DA+G+G VDF+ELA+GL++LCGG R++KVRAAF LYD N DG I +D+M+
Sbjct:   19 QAFHQVELNPDGRIGLDEFKRWYSSPGSHGAATALPSNHL-FTLQVARRLTQLERHAPDTVFELLADCADNNGNLTKDAFSE----CFQVHFLTTTPDARSIAAIHRLFDIFDADGNGTVDFAELAAGLSLLCGGGREDKVRAAFVLYDYNQDGVISLDKMV 175
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: H3GGD8_PHYRM (Uncharacterized protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3GGD8_PHYRM)

HSP 1 Score: 114 bits (286), Expect = 1.660e-26
Identity = 68/166 (40.96%), Postives = 99/166 (59.64%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNGPG-FTIGELQGLLRLHETHVDDVFGQMALASDEHGFIA----EDAFRRELATLTGVAELPAAQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            QAF E D + DG+L  +EF+ WY      G +G    NG   F+I E + L  L      +VF  +A  +DE G+++    +D FR+ +AT  G+    + +  R   I+ RLF + D + +G VDFSE++SGL+V CGG+ +EK+RAAF LYD N DGFI ++EM
Sbjct: 1519 QAFAEADQDHDGQLTLKEFQHWYQQP---GGIGEVAKNGEQLFSIAEARRLTNLQAFSPMEVFETLAECADEQGYVSREAFDDCFRKFIATNEGI---KSEEYQRINPILNRLFELFDVDKNGMVDFSEMSSGLSVFCGGSSEEKIRAAFALYDYNADGFISMEEM 1678
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: G4YZI8_PHYSP (Uncharacterized protein n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G4YZI8_PHYSP)

HSP 1 Score: 113 bits (283), Expect = 4.200e-26
Identity = 67/173 (38.73%), Postives = 95/173 (54.91%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNG------PGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAFRRELATLTGVAELPAAQ------RARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            QAF E D N DG+L+ +EF  WYN+    G       N           + E++ L  L +   ++VF   A  +DE+G ++ DAF      +    +   A+      + + Q +I  LF + D +  G VDF ELASGL+VLCGGT+D+KV AAF+L+D NGDGFI +DEM
Sbjct: 1290 QAFLEADSNHDGKLSYDEFLTWYNSPAQAGISSAVAKNAIVDSSLHWMPLNEIKQLTNLAQYEPEEVFEIFAGEADENGLLSRDAFNESFRKVIAGQKAKGARATSPETQKKLQEVIDGLFDLFDRDNSGAVDFGELASGLSVLCGGTKDQKVEAAFSLFDFNGDGFISLDEM 1462
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BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Match: A0A6G0SMC9_9STRA (Uncharacterized protein n=7 Tax=Phytophthora TaxID=4783 RepID=A0A6G0SMC9_9STRA)

HSP 1 Score: 112 bits (281), Expect = 7.820e-26
Identity = 67/173 (38.73%), Postives = 92/173 (53.18%), Query Frame = 1 
Query:    4 QAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNG------PGFTIGELQGLLRLHETHVDDVFGQMALASDEHGFIAEDAFRRELATLT------GVAELPAAQRARAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYDVNGDGFIGVDEM 486
            QAF E D N DG+L+ +EF  WYN+    G       N           + E++ L  L +   ++VF   A  +DE G ++ DAF      +       G        + + Q +I  LF + D +  G VDF ELASGL+VLCGGT+D+KV AAF+L+D NGDGFI +DEM
Sbjct: 1369 QAFLEADSNHDGKLSYDEFLTWYNSPAQAGISSAVAKNAIVDSSLQWMPLNEIKQLTNLAQYEPEEVFEIFAGEADEDGLLSRDAFNESFRKVIDGQKSKGTRAASPDMQKKLQEVIDGLFDLFDRDNSGAVDFGELASGLSVLCGGTKDQKVEAAFSLFDFNGDGFISLDEM 1541
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig6028.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2D4BN96_PYTIN5.970e-2943.64Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A8K1FFW6_PYTOL8.630e-2941.82Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
K3WY98_GLOUD2.930e-2842.33Uncharacterized protein n=1 Tax=Globisporangium ul... [more]
A0A5D6YAD8_9STRA1.910e-2742.60Uncharacterized protein n=1 Tax=Pythium brassicum ... [more]
A0A6T5TER1_9STRA8.660e-2739.43Hypothetical protein n=1 Tax=Triparma pacifica Tax... [more]
D0N1M0_PHYIT8.940e-2740.36Uncharacterized protein n=1 Tax=Phytophthora infes... [more]
A0A024THC6_9STRA1.370e-2640.74EF-hand domain-containing protein n=1 Tax=Aphanomy... [more]
H3GGD8_PHYRM1.660e-2640.96Uncharacterized protein n=1 Tax=Phytophthora ramor... [more]
G4YZI8_PHYSP4.200e-2638.73Uncharacterized protein n=1 Tax=Phytophthora sojae... [more]
A0A6G0SMC9_9STRA7.820e-2638.73Uncharacterized protein n=7 Tax=Phytophthora TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig6028contigP-wetherbeei_contig6028:2334..3572 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog65071.PYU1_T009947
PFAMsEF-hand_1,EF-hand_5,EF-hand_7,EF-hand_8
Model size489
Max annot lvl121069|Pythiales
Hectar predicted targeting categoryother localisation
Exons4
Evalue4.8e-29
EggNOG OGsCOG5126@1|root,KOG0034@2759|Eukaryota,KOG0044@2759|Eukaryota,1MFBP@121069|Pythiales
DescriptionSource PGD
Cds size489
COG categoryT
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186883.8116589-CDS-P-wetherbeei_contig6028:2333..24311683186883.8116589-CDS-P-wetherbeei_contig6028:2333..2431Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig6028 2334..2431 +
1683186883.8240175-CDS-P-wetherbeei_contig6028:2710..28811683186883.8240175-CDS-P-wetherbeei_contig6028:2710..2881Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig6028 2711..2881 +
1683186883.8355393-CDS-P-wetherbeei_contig6028:3163..33171683186883.8355393-CDS-P-wetherbeei_contig6028:3163..3317Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig6028 3164..3317 +
1683186883.8467422-CDS-P-wetherbeei_contig6028:3506..35721683186883.8467422-CDS-P-wetherbeei_contig6028:3506..3572Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig6028 3507..3572 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig6028.1.1prot_P-wetherbeei_contig6028.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig6028 2334..3572 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig6028.1.1

>prot_P-wetherbeei_contig6028.1.1 ID=prot_P-wetherbeei_contig6028.1.1|Name=mRNA_P-wetherbeei_contig6028.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=163bp
MQAFEEMDINMDGRLNSEEFRCWYNANRCGGSLGGDGDNGPGFTIGELQG
LLRLHETHVDDVFGQMALASDEHGFIAEDAFRRELATLTGVAELPAAQRA
RAQTIIGRLFAVMDANGDGRVDFSELASGLTVLCGGTRDEKVRAAFNLYD
VNGDGFIGVDEML
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mRNA from alignment at P-wetherbeei_contig6028:2334..3572+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig6028.1.1 ID=mRNA_P-wetherbeei_contig6028.1.1|Name=mRNA_P-wetherbeei_contig6028.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1239bp|location=Sequence derived from alignment at P-wetherbeei_contig6028:2334..3572+ (Phaeothamnion wetherbeei SAG_119_79)
ATGCAGGCGTTTGAGGAGATGGATATCAACATGGATGGCCGCCTCAACAG
CGAGGAGTTCCGCTGCTGGTACAATGCTAACAGGTGCGGCGGAAGTCTGT
GTGCTCATTGTGTTGCTCTCGCCTGCTTGACCTGCCGATGCGCTGACGCA
CGCAGTAGCAGTATTAGTATACTCAAGAGCATGGGACGACGTTGAGAATC
GCTGCAGTTGGGATTTGATCCCTCTACTTAATCAACCTCATACGGCGATG
CGAGAATCCATTTGCTATTCTTCTTCCACAGCCTGAGTGCGTCTCTGGCG
GGCATGGAAGCCAACGACCTTCCCGGCGGAGGCGACGGTGACGGCGCTGC
GACCGCCGGCGATTCTGGTGGCGGCAGCGGCGGCGACGGCGACAACGGCC
CCGGTTTCACGATAGGCGAGCTGCAGGGGCTACTGCGGCTGCACGAGACG
CACGTGGACGACGTGTTCGGGCAGATGGCGCTCGCATCGGACGAGCACGG
CTTTATTGCAGAGGACGCCTTCCGGCGGGAGCTCGCCACGCTCACCGGGT
GCGCTTGGCGCGCTTCATTTGGGTAGCAGTCGCTTCAGCGGCTTTCTTAT
TCTCTCAGCCTCTTGGGTGCATTGGCCGCCTCTTAATTGGCTTCGGTTTG
CCGTTGTTCGCGTGCTGTCCTGTTTTGGGCTTGCGCCTCTGTCTCCTTCT
GGTCTACTTCTGATGCCACCTCCATCCGCCTTCCAACTAACAAATTGGCA
CCGTATGCTTCCCCCGATTTCTGCTTTCGACACTGCTTTTTACCAACCTT
CACCCGGGCGGACGGTCACCGTCTTCGCAGGGTGGCGGAGCTGCCGGCGG
CACAGCGGGCTCGGGCGCAGACGATCATTGGGCGACTGTTTGCTGTGATG
GACGCCAACGGCGACGGCCGCGTCGATTTCAGCGAGCTCGCCAGCGGCCT
CACGGTGCTCTGCGGGGGCACCCGCGATGAAAAGGTGCGGCATCGTTCCT
TCTTCCTTCCTAAAAGGCGCGTTGTCTGTGTACCATGCCGACTTGCTGGG
CTGGTGTTCCACGCAGCCTCCATTTTCTTAAAACCTTACTGATTCGTGGC
GCTGTTTCTTGCCTTTCGTAGCTAAATCTGCCTATTCATGGCCCAACCTT
GGAACTTAATTTTCTGACCACAGGTCCGCGCTGCCTTCAACTTGTACGAT
GTGAACGGCGACGGGTTCATCGGGGTGGACGAGATGCTC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig6028:2334..3572+

>mRNA_P-wetherbeei_contig6028.1.1 ID=mRNA_P-wetherbeei_contig6028.1.1|Name=mRNA_P-wetherbeei_contig6028.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=489bp|location=Sequence derived from alignment at P-wetherbeei_contig6028:2334..3572+ (Phaeothamnion wetherbeei SAG_119_79)
ATGCAGGCGTTTGAGGAGATGGATATCAACATGGATGGCCGCCTCAACAG
CGAGGAGTTCCGCTGCTGGTACAATGCTAACAGGTGCGGCGGAAGTCTCG
GCGGCGACGGCGACAACGGCCCCGGTTTCACGATAGGCGAGCTGCAGGGG
CTACTGCGGCTGCACGAGACGCACGTGGACGACGTGTTCGGGCAGATGGC
GCTCGCATCGGACGAGCACGGCTTTATTGCAGAGGACGCCTTCCGGCGGG
AGCTCGCCACGCTCACCGGGGTGGCGGAGCTGCCGGCGGCACAGCGGGCT
CGGGCGCAGACGATCATTGGGCGACTGTTTGCTGTGATGGACGCCAACGG
CGACGGCCGCGTCGATTTCAGCGAGCTCGCCAGCGGCCTCACGGTGCTCT
GCGGGGGCACCCGCGATGAAAAGGTCCGCGCTGCCTTCAACTTGTACGAT
GTGAACGGCGACGGGTTCATCGGGGTGGACGAGATGCTC
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