mRNA_P-wetherbeei_contig51172.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig51172.1.1
Unique NamemRNA_P-wetherbeei_contig51172.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A6H5KBR9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KBR9_9PHAE)

HSP 1 Score: 183 bits (464), Expect = 1.830e-50
Identity = 95/161 (59.01%), Postives = 117/161 (72.67%), Query Frame = 1 
Query:    1 RRQVLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTMA 483
            + QVLLRLF  VF +VT+ P+NE ++RPHL  +VLSCLRYTTRV DP  YY +LR LFR++ GGKF  SY+E+ PLLPL+L E SKLH RA++   K  L ELCL VPARL++L+PHL  MM  M +ALR + G    LALRTLEFWV+NL    LY  M+
Sbjct:  883 KAQVLLRLFKVVFGSVTLFPKNERMLRPHLQTIVLSCLRYTTRVKDPTNYYFLLRALFRSISGGKFESSYKEIYPLLPLLLTELSKLHHRAEEVSIKNILVELCLTVPARLASLLPHLPLMMRLMVHALRCR-GDLDGLALRTLEFWVDNLNPDYLYRIMS 1042
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: D8LFI6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LFI6_ECTSI)

HSP 1 Score: 183 bits (464), Expect = 2.070e-50
Identity = 95/161 (59.01%), Postives = 117/161 (72.67%), Query Frame = 1 
Query:    1 RRQVLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTMA 483
            + QVLLRLF  VF +VT+ P+NE ++RPHL  +VLSCLRYTTRV DP  YY +LR LFR++ GGKF  SY+E+ PLLPL+L E SKLH RA++   K  L ELCL VPARL++L+PHL  MM  M +ALR + G    LALRTLEFWV+NL    LY  M+
Sbjct:  837 KAQVLLRLFKVVFGSVTLFPKNERMLRPHLQTIVLSCLRYTTRVKDPTNYYFLLRALFRSISGGKFESSYKEIYPLLPLLLTELSKLHHRAEEVSIKNILVELCLTVPARLASLLPHLPLMMRLMVHALRCR-GDLDGLALRTLEFWVDNLNPDYLYRIMS 996
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A835YLJ6_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLJ6_9STRA)

HSP 1 Score: 164 bits (414), Expect = 1.860e-46
Identity = 83/152 (54.61%), Postives = 109/152 (71.71%), Query Frame = 1 
Query:    1 RRQVLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQH-GQHRELALRTLEFWVENL 453
            R  VL+RLF  V  +V++   NETV+RPHL  ++LSCLRYTTRV +P  YY +LR LFR++ GGKF  SY+E+ P+LPL+L E SKL++ A D + +  L ELCL VP RL++L+PHL  M+  M  AL +Q  G+   LALRTLEFWV+NL
Sbjct:  115 RASVLMRLFKLVLGSVSLFGPNETVLRPHLQTIILSCLRYTTRVNNPLNYYLLLRALFRSIAGGKFDHSYKEILPILPLLLTELSKLYSTAQDDLTRAILIELCLTVPVRLASLLPHLPLMLRLMVRALSAQQRGELHGLALRTLEFWVDNL 266
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A7S0NXR9_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0NXR9_9EUKA)

HSP 1 Score: 141 bits (356), Expect = 3.510e-37
Identity = 74/158 (46.84%), Postives = 103/158 (65.19%), Query Frame = 1 
Query:   10 VLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTMA 483
            VLLRLF  VF ++T+ PENE V+RPHL  +V + ++Y + +  P  Y+ +LR LF+++GGGKF + Y+E  PLLP +L    + HA A     K  L ELCL +PARLSAL+P+L  +M  + YALRS   +   LALR+LEFW+++L    L   MA
Sbjct:  160 VLLRLFKLVFGSITLFPENEPVLRPHLATIVTTAIKYASCLPYPTNYFSLLRALFKSIGGGKFEQLYKEFLPLLPTLLHGLIRAHACAQQQTLKELLLELCLTLPARLSALLPYLNLLMSPVLYALRSS-AEQISLALRSLEFWIDHLHPDFLQPLMA 316
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A7S4B868_CHRCT (Hypothetical protein (Fragment) n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4B868_CHRCT)

HSP 1 Score: 137 bits (345), Expect = 5.130e-35
Identity = 73/158 (46.20%), Postives = 102/158 (64.56%), Query Frame = 1 
Query:   10 VLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTMA 483
            +LLRLF  VF ++T+ PENE V+RPHL  +V S +RY +    P  Y+ +LR LF+++GGGKF + Y+E  PLLP +L    + +A A     K  L ELCL +PARLSAL+P+L  +M  + +ALRS   Q   +ALR+LEFW+++L    L   MA
Sbjct:  240 MLLRLFKLVFGSITLFPENEPVLRPHLATIVTSAMRYASSQPQPMNYFALLRALFKSIGGGKFEQLYKEFLPLLPRLLHSVIRCYACAQQQALKELLLELCLTLPARLSALLPYLNLLMRPVLHALRSSSEQ-VSMALRSLEFWIDHLHPDFLQPLMA 396
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A0M0KBX1_9EUKA (Transformation transcription domain-associated protein (Fragment) n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0KBX1_9EUKA)

HSP 1 Score: 134 bits (336), Expect = 3.290e-33
Identity = 70/153 (45.75%), Postives = 100/153 (65.36%), Query Frame = 1 
Query:   10 VLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQL 468
            VLLRLF  VF +VT+ PENE ++RPHL  +V   +R+      PA ++ +LR LF+++GGGKF + Y+E  PLLP +L      HA +     +  L ELCL +PARLS+L+P+L  +M  + +ALR+ H +   LALRTLEFW+++L  G L
Sbjct:  494 VLLRLFKLVFGSVTLFPENEPLLRPHLPTIVTQAMRHAAHAAQPANFFSLLRALFKSIGGGKFEQLYKEFLPLLPSLLQGLIGAHAASHQLHVRELLLELCLTLPARLSSLLPYLQLLMRPVLHALRASH-ELISLALRTLEFWIDHLHPGFL 645
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A7R9U8N6_9STRA (Hypothetical protein (Fragment) n=2 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U8N6_9STRA)

HSP 1 Score: 133 bits (334), Expect = 6.290e-33
Identity = 69/157 (43.95%), Postives = 100/157 (63.69%), Query Frame = 1 
Query:   10 VLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTM 480
            +LLRLF   F +VT+  +NE ++RPHL N+V  CLR+      P  ++ +LR LFR++ GGKF  SY EL  LLP++L  F +L+  A D   +  + EL L++P++LS L+PHL  +M  M  ALRS+ G+   L +RT EFWV+NL    LY+ +
Sbjct:  682 ILLRLFKITFGSVTLFHQNEEILRPHLQNIVWGCLRHCAICDAPGNFFVLLRSLFRSISGGKFENSYGELKALLPVILTSFLRLYRSAVDETIRSIIIELSLIIPSKLSYLLPHLPLLMHLMLEALRSE-GELVNLGIRTFEFWVDNLNPDFLYSIL 837
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A0P1AHC8_PLAHL (Phosphatidylinositol kinase (PIK-L3) n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AHC8_PLAHL)

HSP 1 Score: 127 bits (319), Expect = 6.680e-31
Identity = 69/160 (43.12%), Postives = 97/160 (60.62%), Query Frame = 1 
Query:    1 RRQVLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTM 480
            R  ++LRLF  VF +VT+   NE+ + PHL  ++ SCL+  T    P  Y  +LR LFR++ GGK+   Y+E+ PLLP +L    +LH     PV +  L ELCL +PARLS+L+ +L  +M  +  A+ S+ G+   L LRTLEFWV+NL    LY  M
Sbjct:  792 RASIILRLFKIVFGSVTLFKSNESALFPHLRTIIESCLKQATFTKHPDNYLLLLRALFRSISGGKYENFYKEVFPLLPGVLSALMRLHIHISKPVMEEVLLELCLTIPARLSSLLQYLPSLMKSVVRAILSR-GELAYLGLRTLEFWVDNLNPDFLYPIM 950
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: B7FPY1_PHATC (Predicted protein n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7FPY1_PHATC)

HSP 1 Score: 125 bits (314), Expect = 3.130e-30
Identity = 69/164 (42.07%), Postives = 99/164 (60.37%), Query Frame = 1 
Query:    1 RRQVLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTD--PAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTMAK 486
            R    L+LF    K++  +PENE+ +R +L  VV  CLR +   ++  P  Y  +LR +FR++  GKF ESY+EL PL+P +L    ++   ADD + + T  ELCL +PARLS+L+PH+  ++  +  AL S  G    L LRTLEFWV+NL    LY  M+K
Sbjct:  785 RSSTYLQLFERALKSLAPYPENESTIRRYLRFVVSECLRSSLETSELWPDNYCILLRYIFRSISAGKFEESYKELLPLIPTVLNGLYRVICTADDTMLRRTAMELCLTIPARLSSLLPHMNLLVRVIIPALDSNSGDLVNLGLRTLEFWVDNLNPLFLYPEMSK 948
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BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Match: A0A067D1L0_SAPPC (Uncharacterized protein n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067D1L0_SAPPC)

HSP 1 Score: 124 bits (312), Expect = 5.820e-30
Identity = 67/157 (42.68%), Postives = 95/157 (60.51%), Query Frame = 1 
Query:   10 VLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGYYEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTLAELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVENLEHGQLYNTM 480
            ++LRLF  VF +VT+   NET + PHL  ++++CL   T  T P  Y  +LR LFR++ G KF   Y+E+ PLLP +L    +L A    P  +  L ELCL +PARLS+L+ +L  +M  +  A+ S+ G+   L LRTLE+WV+NL    LY  M
Sbjct:  749 IVLRLFKIVFGSVTLFKANETALFPHLKTIIVACLTKATHTTAPDNYLLLLRALFRSISGVKFENFYKEVLPLLPGLLSALMRLQAHIQKPALQDVLLELCLTIPARLSSLLQYLPSLMKSVVAAMLSK-GELANLGLRTLEYWVDNLNPDFLYPIM 904
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig51172.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KBR9_9PHAE1.830e-5059.01Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LFI6_ECTSI2.070e-5059.01Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A835YLJ6_9STRA1.860e-4654.61Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A7S0NXR9_9EUKA3.510e-3746.84Hypothetical protein (Fragment) n=1 Tax=Calcidiscu... [more]
A0A7S4B868_CHRCT5.130e-3546.20Hypothetical protein (Fragment) n=1 Tax=Chrysotila... [more]
A0A0M0KBX1_9EUKA3.290e-3345.75Transformation transcription domain-associated pro... [more]
A0A7R9U8N6_9STRA6.290e-3343.95Hypothetical protein (Fragment) n=2 Tax=Pinguiococ... [more]
A0A0P1AHC8_PLAHL6.680e-3143.13Phosphatidylinositol kinase (PIK-L3) n=1 Tax=Plasm... [more]
B7FPY1_PHATC3.130e-3042.07Predicted protein n=1 Tax=Phaeodactylum tricornutu... [more]
A0A067D1L0_SAPPC5.820e-3042.68Uncharacterized protein n=1 Tax=Saprolegnia parasi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig51172contigP-wetherbeei_contig51172:56..550 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D8LFI6
PFAMsFAT,FATC,PI3_PI4_kinase
Model size495
Max annot lvl2759|Eukaryota
KEGG koko:K08874
KEGG Pathwayko05166,map05166
Hectar predicted targeting categorysignal peptide
GOsGO:0000123,GO:0000166,GO:0003674,GO:0003712,GO:0003824,GO:0004402,GO:0004672,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0006139,GO:0006259,GO:0006281,GO:0006325,GO:0006464,GO:0006468,GO:0006473,GO:0006475,GO:0006725,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0008080,GO:0008144,GO:0008150,GO:0008152,GO:0009889,GO:0009987,GO:0010556,GO:0016043,GO:0016301,GO:0016310,GO:0016407,GO:0016410,GO:0016569,GO:0016570,GO:0016573,GO:0016740,GO:0016746,GO:0016747,GO:0016772,GO:0016773,GO:0017076,GO:0018193,GO:0018205,GO:0018393,GO:0018394,GO:0019219,GO:0019222,GO:0019538,GO:0030554,GO:0031248,GO:0031323,GO:0031326,GO:0031974,GO:0031981,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0033554,GO:0034212,GO:0034641,GO:0035267,GO:0035639,GO:0036094,GO:0036211,GO:0043167,GO:0043168,GO:0043170,GO:0043189,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043543,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051252,GO:0051276,GO:0051716,GO:0060255,GO:0061733,GO:0065007,GO:0070013,GO:0071704,GO:0071840,GO:0080090,GO:0090304,GO:0097159,GO:0097367,GO:0140096,GO:0140110,GO:1901265,GO:1901360,GO:1901363,GO:1901564,GO:1902493,GO:1902494,GO:1902562,GO:1903506,GO:1990234,GO:2001141
Exons1
Evalue3.4e-51
EggNOG OGsCOG5032@1|root,KOG0889@2759|Eukaryota
Descriptioninternal peptidyl-lysine acetylation
Cds size495
COG categoryBDLTU
BRITEko00000,ko00001,ko01001,ko03021,ko03036
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186769.2127619-CDS-P-wetherbeei_contig51172:55..5501683186769.2127619-CDS-P-wetherbeei_contig51172:55..550Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig51172 56..550 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig51172.1.1prot_P-wetherbeei_contig51172.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig51172 56..550 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig51172.1.1

>prot_P-wetherbeei_contig51172.1.1 ID=prot_P-wetherbeei_contig51172.1.1|Name=mRNA_P-wetherbeei_contig51172.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=165bp
RRQVLLRLFAAVFKTVTVHPENETVVRPHLVNVVLSCLRYTTRVTDPAGY
YEVLRQLFRALGGGKFVESYRELAPLLPLMLGEFSKLHARADDPVAKGTL
AELCLMVPARLSALIPHLTRMMPFMAYALRSQHGQHRELALRTLEFWVEN
LEHGQLYNTMAKASL
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mRNA from alignment at P-wetherbeei_contig51172:56..550-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig51172.1.1 ID=mRNA_P-wetherbeei_contig51172.1.1|Name=mRNA_P-wetherbeei_contig51172.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=495bp|location=Sequence derived from alignment at P-wetherbeei_contig51172:56..550- (Phaeothamnion wetherbeei SAG_119_79)
CGCCGCCAGGTGCTGCTCCGGCTGTTTGCGGCCGTCTTCAAGACGGTGAC
GGTGCACCCGGAAAACGAGACGGTGGTGCGGCCTCACCTGGTCAACGTGG
TGCTGTCCTGCCTTCGCTACACGACGCGCGTGACAGATCCTGCCGGCTAC
TACGAGGTGCTGCGCCAATTGTTCCGTGCCCTCGGCGGCGGCAAGTTCGT
TGAATCCTACCGGGAACTGGCGCCCTTGCTGCCGCTGATGCTGGGCGAGT
TCAGCAAGCTGCACGCGCGCGCCGACGACCCCGTCGCGAAGGGCACGCTG
GCTGAGCTGTGCCTGATGGTGCCGGCGCGCCTGTCGGCGCTGATCCCGCA
CCTGACGCGCATGATGCCGTTCATGGCATACGCGCTACGCAGCCAGCATG
GGCAGCATCGCGAGCTGGCGCTGCGGACGCTCGAGTTCTGGGTAGAGAAT
CTCGAGCACGGACAGCTCTACAACACCATGGCCAAGGCTAGTCTC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig51172:56..550-

>mRNA_P-wetherbeei_contig51172.1.1 ID=mRNA_P-wetherbeei_contig51172.1.1|Name=mRNA_P-wetherbeei_contig51172.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=495bp|location=Sequence derived from alignment at P-wetherbeei_contig51172:56..550- (Phaeothamnion wetherbeei SAG_119_79)
CGCCGCCAGGTGCTGCTCCGGCTGTTTGCGGCCGTCTTCAAGACGGTGAC
GGTGCACCCGGAAAACGAGACGGTGGTGCGGCCTCACCTGGTCAACGTGG
TGCTGTCCTGCCTTCGCTACACGACGCGCGTGACAGATCCTGCCGGCTAC
TACGAGGTGCTGCGCCAATTGTTCCGTGCCCTCGGCGGCGGCAAGTTCGT
TGAATCCTACCGGGAACTGGCGCCCTTGCTGCCGCTGATGCTGGGCGAGT
TCAGCAAGCTGCACGCGCGCGCCGACGACCCCGTCGCGAAGGGCACGCTG
GCTGAGCTGTGCCTGATGGTGCCGGCGCGCCTGTCGGCGCTGATCCCGCA
CCTGACGCGCATGATGCCGTTCATGGCATACGCGCTACGCAGCCAGCATG
GGCAGCATCGCGAGCTGGCGCTGCGGACGCTCGAGTTCTGGGTAGAGAAT
CTCGAGCACGGACAGCTCTACAACACCATGGCCAAGGCTAGTCTC
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