mRNA_P-wetherbeei_contig20421.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig20421.1.1
Unique NamemRNA_P-wetherbeei_contig20421.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: Q9SMD8_9PHAE (Glycolate oxidase (Fragment) n=1 Tax=Laminaria digitata TaxID=80365 RepID=Q9SMD8_9PHAE)

HSP 1 Score: 203 bits (516), Expect = 7.870e-64
Identity = 97/127 (76.38%), Postives = 107/127 (84.25%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYSKL 381
            ASEA++ GVD IW+SNHGARQLDT  ATIE LPE+V+AV GRCEVYLDGGICRGTDVFKA+ALG  AVFIGRPVLWGL HSGE+GV  VLKLLN EL MAL L GCT++   T +MVTHQ SYYSKL
Sbjct:  113 ASEAVRQGVDGIWISNHGARQLDTTPATIEVLPEVVQAVSGRCEVYLDGGICRGTDVFKAIALGAKAVFIGRPVLWGLGHSGEEGVSKVLKLLNDELIMALQLTGCTRISAATRAMVTHQISYYSKL 239
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: D8LDI6_ECTSI (Glycolate Oxidase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LDI6_ECTSI)

HSP 1 Score: 204 bits (518), Expect = 3.260e-62
Identity = 98/127 (77.17%), Postives = 108/127 (85.04%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYSKL 381
            A+E+++ GVD IWVSNHGARQLDT  ATIE LPE+V AV GRCE+YLDGGICRGTDVFKALALG  AVFIGRPVLWGLAHSGE+GV  VLKLL+ EL MAL L GCT+V   + SMVTHQTSYYSKL
Sbjct:  268 AAESVRQGVDGIWVSNHGARQLDTTPATIEVLPEVVAAVSGRCEIYLDGGICRGTDVFKALALGAKAVFIGRPVLWGLAHSGEEGVSKVLKLLHDELVMALQLTGCTRVSSASRSMVTHQTSYYSKL 394
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: W7UBQ6_9STRA (Peroxisomal glycolate oxidase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7UBQ6_9STRA)

HSP 1 Score: 203 bits (517), Expect = 4.160e-62
Identity = 96/124 (77.42%), Postives = 106/124 (85.48%), Query Frame = 1 
Query:   10 ALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYSKL 381
            A++CGVDAIWVSNHGARQLDTVA+TIE LP+IV+AV GRCEVYLDGG+CRGTD FKALALG  AVFIGRPVLWGLAHSGEDGV  VL+LL  E EMA+ L GCTK+ DI  SMVTH  SY SK+
Sbjct:  267 AMQCGVDAIWVSNHGARQLDTVASTIELLPDIVKAVNGRCEVYLDGGVCRGTDAFKALALGARAVFIGRPVLWGLAHSGEDGVYQVLELLKKEFEMAMQLTGCTKLSDIQRSMVTHVVSYQSKI 390
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: A0A836CMB2_9STRA (Glycolate oxidase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CMB2_9STRA)

HSP 1 Score: 197 bits (501), Expect = 1.250e-59
Identity = 95/125 (76.00%), Postives = 107/125 (85.60%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYS 375
            A EAL+ GVDAIWVSNHGARQLDTV++TIEALPE+V AV G+ EVYLDGG+ RGTDVFKALA+G  AVFIGRPVLWGLAHSGEDGV  VL+L+N EL MAL LAGCT V +I  SMV HQT+YY+
Sbjct:  269 AREALRYGVDAIWVSNHGARQLDTVSSTIEALPEVVAAVAGKVEVYLDGGVTRGTDVFKALAMGARAVFIGRPVLWGLAHSGEDGVAAVLRLMNEELTMALQLAGCTSVDEIQKSMVVHQTAYYT 393
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: A0A7R9UGG8_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UGG8_9STRA)

HSP 1 Score: 193 bits (491), Expect = 3.570e-58
Identity = 92/127 (72.44%), Postives = 102/127 (80.31%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYSKL 381
            AS A++ GVDAIW+SNHGARQLDT  AT E +PEI+ AVGGRCEVY+DGGI RG D  KA+ALGC AVFIGRPVLWGLAHSG DGV HVL LL AELEMA+ML G   VKDI   MVTH T Y+S+L
Sbjct:  267 ASVAVREGVDAIWISNHGARQLDTTPATCEMIPEIIAAVGGRCEVYVDGGITRGADALKAVALGCRAVFIGRPVLWGLAHSGSDGVEHVLDLLKAELEMAMMLCGAVTVKDIKREMVTHNTKYFSRL 393
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: A0A7S1UI71_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UI71_9STRA)

HSP 1 Score: 187 bits (475), Expect = 9.100e-56
Identity = 88/127 (69.29%), Postives = 101/127 (79.53%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYSKL 381
            A+ A++ GVDAIW+SNHGARQLDT  AT E +PE+V AVGGRCEVY+DGGI RG D  KA+ALGCTAVF+GRPVLWGLAHSG+ GV  VL LL AE EMA+ML GC  V DI   MVTH T +YS+L
Sbjct:  268 AAIAVREGVDAIWISNHGARQLDTTPATCEMIPEVVAAVGGRCEVYVDGGITRGADALKAVALGCTAVFVGRPVLWGLAHSGQAGVEKVLDLLKAEFEMAMMLCGCVTVDDIKREMVTHATKFYSRL 394
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: A0A7S4KCV1_9STRA (Hypothetical protein n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4KCV1_9STRA)

HSP 1 Score: 187 bits (475), Expect = 1.000e-55
Identity = 90/127 (70.87%), Postives = 103/127 (81.10%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYSKL 381
            A EA+KC VDAIWVSNHGARQLDTVA+TIE LP I + V GRCE+Y+DGGI RGTD+FKALALG  AVFIGRPVLW LAHSGE+GV+HV+ LL  E E+AL L+GCT + DI   MVTH  S YS+L
Sbjct:  273 AEEAVKCKVDAIWVSNHGARQLDTVASTIELLPSIAKQVNGRCEIYVDGGIYRGTDIFKALALGARAVFIGRPVLWSLAHSGEEGVKHVIGLLKDEFELALSLSGCTSLSDIQQEMVTH-VSQYSRL 398
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: D7FMI7_ECTSI (Glycolate Oxidase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FMI7_ECTSI)

HSP 1 Score: 184 bits (468), Expect = 1.290e-54
Identity = 89/127 (70.08%), Postives = 103/127 (81.10%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSYYSKL 381
            A EAL  GVDA+WVSNHG RQLDTV ATIE LPE+V+AV GRCE+++DGGI RGTDV KALALG +AVFIGRPV+WGLAHSGE GV  V+ LLN EL  A+ L GC K+ DI  SMV HQ+SY++KL
Sbjct:  278 AEEALAHGVDAVWVSNHGGRQLDTVPATIEILPEVVQAVRGRCEIFVDGGIRRGTDVLKALALGASAVFIGRPVIWGLAHSGEHGVTDVINLLNEELVQAMRLMGCKKLGDIERSMVAHQSSYHAKL 404
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: A0A6V1QYU9_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1QYU9_HETAK)

HSP 1 Score: 180 bits (456), Expect = 6.160e-53
Identity = 85/123 (69.11%), Postives = 98/123 (79.67%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSY 369
            A  A +  VD IWVSNHGARQLDT  ATIE +PEIV AV  RCE+Y+DGG+ RGTD FKALALGCTAVFIGRPVLWGLAHSGE+GV HVL+LL AE EMA+ L GCT+V +I  +MV H + +
Sbjct:  268 ARMACEANVDGIWVSNHGARQLDTCLATIEMVPEIVAAVNKRCEIYVDGGVLRGTDAFKALALGCTAVFIGRPVLWGLAHSGEEGVAHVLQLLRAEFEMAMQLNGCTRVGEIQRAMVCHASEF 390
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BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Match: A0A7S2V919_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V919_9STRA)

HSP 1 Score: 175 bits (443), Expect = 1.380e-51
Identity = 86/123 (69.92%), Postives = 95/123 (77.24%), Query Frame = 1 
Query:    1 ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGGICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMALMLAGCTKVKDITSSMVTHQTSY 369
            A  A++  VD IWVSNHGARQLDTVAATIE LPE+VRAV G CEVY+DGGI RGTDVFKALALG  AVFIGRPVLWGLAHSGE+GV + L LL  E  MA+ L GC KV DI  + V H + Y
Sbjct:  212 AKVAVENKVDGIWVSNHGARQLDTVAATIEMLPEVVRAVNGACEVYVDGGILRGTDVFKALALGAKAVFIGRPVLWGLAHSGEEGVTNALTLLQNEFTMAMQLTGCVKVDDINRAHVCHASEY 334
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig20421.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Q9SMD8_9PHAE7.870e-6476.38Glycolate oxidase (Fragment) n=1 Tax=Laminaria dig... [more]
D8LDI6_ECTSI3.260e-6277.17Glycolate Oxidase n=2 Tax=Ectocarpus TaxID=2879 Re... [more]
W7UBQ6_9STRA4.160e-6277.42Peroxisomal glycolate oxidase n=2 Tax=Monodopsidac... [more]
A0A836CMB2_9STRA1.250e-5976.00Glycolate oxidase n=1 Tax=Tribonema minus TaxID=30... [more]
A0A7R9UGG8_9STRA3.570e-5872.44Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A7S1UI71_9STRA9.100e-5669.29Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]
A0A7S4KCV1_9STRA1.000e-5570.87Hypothetical protein n=1 Tax=Odontella aurita TaxI... [more]
D7FMI7_ECTSI1.290e-5470.08Glycolate Oxidase n=2 Tax=Ectocarpus TaxID=2879 Re... [more]
A0A6V1QYU9_HETAK6.160e-5369.11Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A7S2V919_9STRA1.380e-5169.92Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig20421contigP-wetherbeei_contig20421:5..620 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog2880.D7FMI7
Preferred nameGOX
PFAMsFMN_dh
Model size384
Max annot lvl2759|Eukaryota
KEGG rclassRC00042
KEGG koko:K11517,ko:K13344
KEGG TC3.A.20.1
KEGG ReactionR00475
KEGG Pathwayko00630,ko01100,ko01110,ko01120,ko01130,ko01200,ko04146,map00630,map01100,map01110,map01120,map01130,map01200,map04146
KEGG ModuleM00532
Hectar predicted targeting categoryother localisation
GOsGO:0002218,GO:0002253,GO:0002376,GO:0002682,GO:0002684,GO:0003674,GO:0003824,GO:0003973,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005777,GO:0005829,GO:0005840,GO:0005911,GO:0006950,GO:0006952,GO:0007154,GO:0007165,GO:0008150,GO:0008152,GO:0008891,GO:0009058,GO:0009506,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009605,GO:0009607,GO:0009617,GO:0009719,GO:0009725,GO:0009735,GO:0009853,GO:0009987,GO:0010033,GO:0010109,GO:0010204,GO:0016020,GO:0016032,GO:0016491,GO:0016614,GO:0016899,GO:0016999,GO:0017000,GO:0017144,GO:0019048,GO:0019222,GO:0022626,GO:0023052,GO:0030054,GO:0031323,GO:0031347,GO:0031349,GO:0032991,GO:0035821,GO:0042221,GO:0042579,GO:0042742,GO:0042743,GO:0043094,GO:0043207,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0044003,GO:0044237,GO:0044249,GO:0044403,GO:0044419,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044445,GO:0044446,GO:0044464,GO:0045088,GO:0045089,GO:0048046,GO:0048518,GO:0048583,GO:0048584,GO:0050665,GO:0050776,GO:0050778,GO:0050789,GO:0050794,GO:0050896,GO:0051186,GO:0051188,GO:0051701,GO:0051704,GO:0051707,GO:0051716,GO:0051817,GO:0055044,GO:0055114,GO:0065007,GO:0072593,GO:0080134,GO:0098542,GO:1903409,GO:1990904
Exons2
Evalue2.11e-55
EggNOG OGsCOG1304@1|root,KOG0538@2759|Eukaryota
Ec32 ortholog descriptionGlycolate Oxidase
Ec32 orthologEc-23_000460.1
EC1.1.3.15
Descriptionvery-long-chain-(S)-2-hydroxy-acid oxidase activity
Cds size384
COG categoryC
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186199.3687618-CDS-P-wetherbeei_contig20421:4..2471683186199.3687618-CDS-P-wetherbeei_contig20421:4..247Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig20421 5..247 +
1683186199.381462-CDS-P-wetherbeei_contig20421:479..6201683186199.381462-CDS-P-wetherbeei_contig20421:479..620Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig20421 480..620 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig20421.1.1prot_P-wetherbeei_contig20421.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig20421 5..620 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig20421.1.1

>prot_P-wetherbeei_contig20421.1.1 ID=prot_P-wetherbeei_contig20421.1.1|Name=mRNA_P-wetherbeei_contig20421.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=128bp
ASEALKCGVDAIWVSNHGARQLDTVAATIEALPEIVRAVGGRCEVYLDGG
ICRGTDVFKALALGCTAVFIGRPVLWGLAHSGEDGVRHVLKLLNAELEMA
LMLAGCTKVKDITSSMVTHQTSYYSKL*
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mRNA from alignment at P-wetherbeei_contig20421:5..620+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig20421.1.1 ID=mRNA_P-wetherbeei_contig20421.1.1|Name=mRNA_P-wetherbeei_contig20421.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=616bp|location=Sequence derived from alignment at P-wetherbeei_contig20421:5..620+ (Phaeothamnion wetherbeei SAG_119_79)
GCCTCCGAGGCGCTCAAGTGCGGCGTCGACGCCATCTGGGTCTCCAACCA
CGGCGCGCGGCAGCTGGACACGGTGGCGGCGACGATCGAGGCACTGCCGG
AGATTGTGCGGGCTGTCGGCGGCCGCTGCGAGGTGTACCTTGACGGCGGA
ATCTGCCGCGGGACGGACGTGTTCAAGGCTCTGGCGCTTGGCTGCACGGC
CGTGTTCATTGGCCGGCCGGTCTTGTGGGGCCTCGCACATAGCGTGAGTG
GGCAGGCTTTTTCTGACCACCCTTTTTGTGCTCGCACGGCTTCCTTCCCG
CCTCTCGCGCTTACTGCATGGTTGCGTGCGGCTCTTTTCTTCGGTCGAAC
CCGGTGATAGAGAGAAGAGGGAGCGAAGGAGATCTCGCGTACCCTTTCCG
TCTCTTTTATGAGCACTTTTCTGCCTTTGCTCTTTTTTCTTCTTTTCTCT
TCCTCTTCTCTGCGCCGTCCTGCAGGGCGAGGACGGAGTGCGGCACGTAC
TGAAGCTCCTGAACGCGGAGCTGGAGATGGCGCTGATGCTTGCCGGCTGC
ACCAAGGTGAAGGACATCACCTCCTCCATGGTCACCCACCAAACCTCATA
CTACTCCAAGCTCTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig20421:5..620+

>mRNA_P-wetherbeei_contig20421.1.1 ID=mRNA_P-wetherbeei_contig20421.1.1|Name=mRNA_P-wetherbeei_contig20421.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=384bp|location=Sequence derived from alignment at P-wetherbeei_contig20421:5..620+ (Phaeothamnion wetherbeei SAG_119_79)
GCCTCCGAGGCGCTCAAGTGCGGCGTCGACGCCATCTGGGTCTCCAACCA
CGGCGCGCGGCAGCTGGACACGGTGGCGGCGACGATCGAGGCACTGCCGG
AGATTGTGCGGGCTGTCGGCGGCCGCTGCGAGGTGTACCTTGACGGCGGA
ATCTGCCGCGGGACGGACGTGTTCAAGGCTCTGGCGCTTGGCTGCACGGC
CGTGTTCATTGGCCGGCCGGTCTTGTGGGGCCTCGCACATAGCGGCGAGG
ACGGAGTGCGGCACGTACTGAAGCTCCTGAACGCGGAGCTGGAGATGGCG
CTGATGCTTGCCGGCTGCACCAAGGTGAAGGACATCACCTCCTCCATGGT
CACCCACCAAACCTCATACTACTCCAAGCTCTGA
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