mRNA_P-wetherbeei_contig1308.4.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig1308.4.1
Unique NamemRNA_P-wetherbeei_contig1308.4.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A835YLA7_9STRA (Pseudouridine synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLA7_9STRA)

HSP 1 Score: 135 bits (340), Expect = 2.750e-33
Identity = 74/151 (49.01%), Postives = 88/151 (58.28%), Query Frame = 1
Query:    7 QIGWVR--------------ASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVK 417
            +IGW R              ASRTD GVHAAK V+ AKLLVDQT+F   G +  L+  INA LPPDLRVFSVCKVPK FR R EC+WR YEYYLP  +L +                       +   A+F  LLRL+ GTHSFHNF+++K
Sbjct:   67 KIGWSRTSRIPLDGFVNIAYASRTDKGVHAAKTVVVAKLLVDQTQFCAAGLNAPLKAAINACLPPDLRVFSVCKVPKGFRGRGECSWRDYEYYLPASLLTD-----------------------DKATAKFGELLRLFEGTHSFHNFISMK 194          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: D8LDS4_ECTSI (Pseudouridylate synthase, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDS4_ECTSI)

HSP 1 Score: 124 bits (311), Expect = 3.050e-29
Identity = 68/137 (49.64%), Postives = 83/137 (60.58%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVK 417
            +IGW R SRTD GVHA+K V AAKLL+ Q  F   G S+  +  +N +LP D+R FSV KVPKSFR R ECT R Y YYLP E+L             DDE+           L +FR LL+L+VGTHSFHN+ + K
Sbjct:   81 KIGWGRTSRTDKGVHASKTVAAAKLLLPQETFTATGMSQYGKDKLNEKLPDDIRCFSVSKVPKSFRGREECTLREYTYYLPRELLP------------DDEA-----------LGKFRELLKLFVGTHSFHNYASTK 194          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A6H5L6M3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L6M3_9PHAE)

HSP 1 Score: 123 bits (308), Expect = 7.440e-29
Identity = 65/137 (47.45%), Postives = 81/137 (59.12%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVK 417
            +IGW R SRTD GVHA+K V AAKLL+ Q  F   G S+  +  +N  LP D+R FSV KVPKSFR R ECT R Y YYLP E+L +                       + +L +FR LL+L+VGTHSFHN+ + K
Sbjct:  160 KIGWGRTSRTDKGVHASKTVAAAKLLLPQETFTAAGMSQYGKDKLNENLPDDIRCFSVSKVPKSFRGREECTLREYTYYLPRELLPD-----------------------DGSLGKFRELLKLFVGTHSFHNYASTK 273          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A7S2NDW7_9DINO (Hypothetical protein (Fragment) n=1 Tax=Brandtodinium nutricula TaxID=1333877 RepID=A0A7S2NDW7_9DINO)

HSP 1 Score: 100 bits (250), Expect = 7.200e-23
Identity = 54/137 (39.42%), Postives = 78/137 (56.93%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVK 417
            +IGW R+SRTDAGV A + +++A+ LV +  F            +N ELP D+R F   +VP+SF A+R C+WR YEY LP EM     PA         + G   ++D E+ +A  +A +R + G HS+HNF  V+
Sbjct:   62 KIGWSRSSRTDAGVSAVRLIISARFLVSEDGFDAADHHSATVEELNRELPHDIRCFGAVRVPRSFDAKRSCSWREYEYLLPVEMCR---PAT--------QDGAAPIAD-EALVASVQAAMRRFEGNHSYHNFSRVR 186          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: W7U6V3_9STRA (Pseudouridylate synthase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U6V3_9STRA)

HSP 1 Score: 101 bits (251), Expect = 3.360e-21
Identity = 73/184 (39.67%), Postives = 98/184 (53.26%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKL--LVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVKKARKDRERGREERAAAMAAXXXXXXVTGSDAAAVVAGIAIAVGED 552
            +IGW R SRTD GVHAA+NV++AKL   VD  +      SE   + INA LP D+RV S  K  KSFR R   ++R Y Y +P E+L    P   T  +  DES       +E+ +ARF  LL  + GTH+FHNF  +K   +D  R  + +A +  A        G +  A+ AG   + GED
Sbjct:  175 KIGWARCSRTDKGVHAARNVVSAKLELWVDDMEGDSPNWSERAVKEINACLPDDIRVHSCAKATKSFRPRDAVSFREYVYIVPLELLR---PFPGTGRAVADES-------SEALIARFCDLLVRFQGTHNFHNFTKIKS--RDLIRRLKAKAGSSRAWVHNNQRGGREGEAIAAGGEASTGED 346          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A2D4BJ04_PYTIN (PseudoU_synth_1 domain-containing protein n=2 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BJ04_PYTIN)

HSP 1 Score: 99.0 bits (245), Expect = 1.890e-20
Identity = 54/139 (38.85%), Postives = 77/139 (55.40%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRD--GASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVK 417
            +I W R+SRTD GVHA   V +AKLL+D+        G    L  ++NA+LPP +R+FS  +V K F ARR C  R YEY+LP   L+ ++ + +     DD+    +  D +  +  F + LR Y G H FHNF   +
Sbjct:   99 KIDWSRSSRTDKGVHAGCIVFSAKLLIDEEAAVDPVTGRVNSLVESLNAQLPPTIRIFSATRVNKRFSARRNCVLREYEYFLP---LSFLEQSFLATALNDDDDATRTDIDIDEAVNEFLSALREYEGIHDFHNFTKAR 234          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A250X951_9CHLO (PseudoU_synth_1 domain-containing protein n=1 Tax=Chlamydomonas eustigma TaxID=1157962 RepID=A0A250X951_9CHLO)

HSP 1 Score: 98.2 bits (243), Expect = 3.780e-20
Identity = 57/139 (41.01%), Postives = 76/139 (54.68%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRT--INAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVK 417
            ++ W RASRTD GVH+   V++ +LLV   ++  +G  E L     INA LPP +RVF+V K  K F AR     R YEYYLP  ML       +  D  +     H V      +A FR +LRL++G+H FHNF  +K
Sbjct:   70 KVKWARASRTDKGVHSLGTVMSMRLLVHDNRYSPEGDPEGLTHADLINAHLPPTIRVFTVQKTNKKFNARHLALSRIYEYYLPVSMLE------LKMDGSE-----HDVE----RIATFRRILRLFLGSHPFHNFTKMK 193          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A2P6TFJ8_CHLSO (tRNA pseudouridine synthase n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TFJ8_CHLSO)

HSP 1 Score: 94.4 bits (233), Expect = 8.870e-19
Identity = 56/159 (35.22%), Postives = 79/159 (49.69%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARF---RALLRLYVGTHSFHNFVTVKKARKD------RERGREER 456
            ++ W R+SRTD  VH+   V+A K+ V+   F  D   + L   INA LPP++RVFS+ +V KS+ AR EC  RCY YYLP  +L                  G  +  +E+  AR    R     + GTH FHN+   +  R+       R +GR +R
Sbjct:  655 KVSWSRSSRTDKSVHSLATVVAMKMEVEPESFDTDPEGQALAAAINASLPPEVRVFSIQRVSKSWNARSECIRRCYNYYLPASIL------------------GLKLDGSEADAARLELLRTAWERFQGTHPFHNYTKRRLYREPAAASDGRRKGRRDR 795          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A2P6VG62_9CHLO (tRNA pseudouridine synthase n=1 Tax=Micractinium conductrix TaxID=554055 RepID=A0A2P6VG62_9CHLO)

HSP 1 Score: 92.8 bits (229), Expect = 2.020e-18
Identity = 52/150 (34.67%), Postives = 77/150 (51.33%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVKKARKDRERGREER 456
            ++GW R+SRTD  VH+   V+  K+ V    F  D   +EL   +N+ LPP++RVFS+ +V KS+ AR EC  R Y Y+LP   L       +  D G+ ++         + +A  RA    + GTH FHN+   +  R+   R R  R
Sbjct:   35 KVGWSRSSRTDKSVHSLSTVIGMKMEVPVEAFDADPEGQELAAVVNSHLPPEVRVFSIQRVSKSWNARSECIRRTYNYWLPASALG------LALDGGEADA---------ARMALLRAAWERFQGTHPFHNYTKRRLYREPAGRDRANR 169          
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Match: A0A6G0WJY3_9STRA (PseudoU_synth_1 domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WJY3_9STRA)

HSP 1 Score: 92.0 bits (227), Expect = 3.380e-18
Identity = 54/148 (36.49%), Postives = 79/148 (53.38%), Query Frame = 1
Query:    7 QIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAELPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSGDDESGGHSVS-DTES------------TLARFRALLRLYVGTHSFHNFVT 411
            +IGW RA+RTD GVHAA  ++ AKL ++      DG   E R+ +N  LPPD++V  +  V K+F A+  C  R YEY  P  + A   P   ++   +      S+  D E+            TLA+ R  L+L+VGTH+FHN+ +
Sbjct:  117 KIGWNRAARTDKGVHAAGQLVCAKLCIE------DGKEVEFRQKVNGFLPPDIQVLDIVLVTKNFNAKNSCDRRTYEYLTPTFVFA---PKTASSTPPEGAVDLESIKVDPEAWAKQKEYRIDNDTLAKLRQTLKLFVGTHNFHNYTS 255          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig1308.4.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YLA7_9STRA2.750e-3349.01Pseudouridine synthase n=1 Tax=Tribonema minus Tax... [more]
D8LDS4_ECTSI3.050e-2949.64Pseudouridylate synthase, putative n=1 Tax=Ectocar... [more]
A0A6H5L6M3_9PHAE7.440e-2947.45Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S2NDW7_9DINO7.200e-2339.42Hypothetical protein (Fragment) n=1 Tax=Brandtodin... [more]
W7U6V3_9STRA3.360e-2139.67Pseudouridylate synthase n=2 Tax=Monodopsidaceae T... [more]
A0A2D4BJ04_PYTIN1.890e-2038.85PseudoU_synth_1 domain-containing protein n=2 Tax=... [more]
A0A250X951_9CHLO3.780e-2041.01PseudoU_synth_1 domain-containing protein n=1 Tax=... [more]
A0A2P6TFJ8_CHLSO8.870e-1935.22tRNA pseudouridine synthase n=1 Tax=Chlorella soro... [more]
A0A2P6VG62_9CHLO2.020e-1834.67tRNA pseudouridine synthase n=1 Tax=Micractinium c... [more]
A0A6G0WJY3_9STRA3.380e-1836.49PseudoU_synth_1 domain-containing protein n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig1308contigP-wetherbeei_contig1308:6229..7399 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D8LDS4
Preferred namePUS1
PFAMsPseudoU_synth_1
Model size630
Max annot lvl2759|Eukaryota
KEGG koko:K06173,ko:K14016,ko:K20674
KEGG Pathwayko04141,ko04624,map04141,map04624
KEGG ModuleM00400,M00403
Hectar predicted targeting categoryother localisation
GOsGO:0000049,GO:0000959,GO:0000963,GO:0001522,GO:0002153,GO:0003674,GO:0003676,GO:0003712,GO:0003713,GO:0003723,GO:0003727,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005667,GO:0005730,GO:0005737,GO:0005739,GO:0005759,GO:0006139,GO:0006355,GO:0006357,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009451,GO:0009889,GO:0009891,GO:0009893,GO:0009982,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0016070,GO:0016071,GO:0016556,GO:0016853,GO:0016866,GO:0019219,GO:0019222,GO:0030374,GO:0031119,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031974,GO:0031981,GO:0032991,GO:0034470,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0045893,GO:0045935,GO:0045944,GO:0046483,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0060255,GO:0065007,GO:0070013,GO:0070525,GO:0070900,GO:0070902,GO:0071704,GO:0080090,GO:0090304,GO:0090646,GO:0097159,GO:0106029,GO:0140053,GO:0140098,GO:0140101,GO:0140110,GO:1900864,GO:1901360,GO:1901363,GO:1902680,GO:1903506,GO:1903508,GO:1990481,GO:2000112,GO:2001141
Exons2
Evalue5e-30
EggNOG OGsCOG0101@1|root,KOG2553@2759|Eukaryota
Ec32 ortholog descriptionPseudouridine synthase I, TruA, alpha/beta domain
Ec32 orthologEc-08_003150.1
EC5.4.99.12
Descriptionpseudouridine synthase activity
Cds size630
COG categoryJ
BRITEko00000,ko00001,ko00002,ko01000,ko01002,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185973.3396337-CDS-P-wetherbeei_contig1308:6228..66721683185973.3396337-CDS-P-wetherbeei_contig1308:6228..6672Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1308 6229..6672 -
1683185973.3492196-CDS-P-wetherbeei_contig1308:7213..73991683185973.3492196-CDS-P-wetherbeei_contig1308:7213..7399Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1308 7214..7399 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig1308.4.1prot_P-wetherbeei_contig1308.4.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig1308 6229..7399 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig1308.4.1

>prot_P-wetherbeei_contig1308.4.1 ID=prot_P-wetherbeei_contig1308.4.1|Name=mRNA_P-wetherbeei_contig1308.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=210bp
HPQIGWVRASRTDAGVHAAKNVLAAKLLVDQTKFGRDGASEELRRTINAE
LPPDLRVFSVCKVPKSFRARRECTWRCYEYYLPWEMLANVDPAVVTADSG
DDESGGHSVSDTESTLARFRALLRLYVGTHSFHNFVTVKKARKDRERGRE
ERAAAMAATAAAAAVTGSDAAAVVAGIAIAVGEDLETDDDVEFVDDVEVD
GFVDDDDDGG
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mRNA from alignment at P-wetherbeei_contig1308:6229..7399-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig1308.4.1 ID=mRNA_P-wetherbeei_contig1308.4.1|Name=mRNA_P-wetherbeei_contig1308.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1171bp|location=Sequence derived from alignment at P-wetherbeei_contig1308:6229..7399- (Phaeothamnion wetherbeei SAG_119_79)
CATCCGCAGATCGGCTGGGTGCGCGCATCGCGGACGGACGCGGGCGTGCA CGCTGCCAAGAACGTGTTGGCGGCGAAGCTGCTGGTGGACCAAACAAAAT TCGGCCGCGACGGCGCCTCCGAAGAGCTGCGGCGGACCATCAACGCAGAG TTGCCGCCCGACCTCCGCGTCTTCTCCGTCTGCAAGGTGGGCGACCTGGC AGTGTAACGGTCCGTATGTAACGGCCTGCATGTAACAGTCTGTGTATAAA ATCTGTATGTAAGTTATTGGCGTTATTGGCGGGCGGAGGGGACTTCTTTT GCCGAAGCAGCTTTCGGCGGTAGCATCTGTGACTGTCACGGCCAGTTTCG TCTCATTTGTGGAGGCATGGTTAGAGTTCTCACAAGACTGCTTGAAAGCG GCTGACAGAGCACACAGTCTTGCCGGGCCATGCAATGTCGGATGCGACTT TAGCCCTTGAGTTAATGTCCAATCGCGAGTTGCGGAGACTAGGACCCGCC GGAAGCGGCGTTGGCGCGATGTCGGAGGGCTGACCTAAAACACAATTGGA AAGACTAAAGGACGCGAAAGCCTTTGCTTCTTCAGTCCTTGCTGTCGAGG TTGACGCTTAGGGCTCCAAGATGTCCTCGCCGGAAAGCTGCCCGTTTCTT GTCCCGGTGCCGCAAATATGACATATCACGCATGCCCCGCGATTTTTCTC GTCGTCCGTCTCTACGGACCTGAGCAGGTTCCGAAGAGCTTCCGGGCGCG CCGCGAGTGCACTTGGCGTTGCTACGAATACTATCTTCCGTGGGAGATGC TGGCAAACGTTGACCCAGCGGTGGTAACGGCCGACAGCGGTGATGACGAG AGCGGTGGCCACAGTGTCTCCGACACCGAGTCGACGCTCGCGCGGTTCCG CGCGCTGCTCAGGCTCTACGTCGGGACTCACAGCTTTCACAATTTTGTCA CAGTTAAAAAGGCCCGGAAGGACAGGGAACGCGGCCGGGAAGAGCGCGCG GCGGCGATGGCGGCGACGGCGGCGGCTGCGGCGGTCACCGGGTCCGATGC AGCTGCGGTGGTTGCTGGCATTGCAATAGCCGTCGGAGAAGACTTGGAAA CCGACGACGACGTAGAGTTTGTGGACGATGTCGAGGTAGACGGTTTTGTC GATGACGACGACGACGGCGGC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig1308:6229..7399-

>mRNA_P-wetherbeei_contig1308.4.1 ID=mRNA_P-wetherbeei_contig1308.4.1|Name=mRNA_P-wetherbeei_contig1308.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=630bp|location=Sequence derived from alignment at P-wetherbeei_contig1308:6229..7399- (Phaeothamnion wetherbeei SAG_119_79)
CATCCGCAGATCGGCTGGGTGCGCGCATCGCGGACGGACGCGGGCGTGCA
CGCTGCCAAGAACGTGTTGGCGGCGAAGCTGCTGGTGGACCAAACAAAAT
TCGGCCGCGACGGCGCCTCCGAAGAGCTGCGGCGGACCATCAACGCAGAG
TTGCCGCCCGACCTCCGCGTCTTCTCCGTCTGCAAGGTTCCGAAGAGCTT
CCGGGCGCGCCGCGAGTGCACTTGGCGTTGCTACGAATACTATCTTCCGT
GGGAGATGCTGGCAAACGTTGACCCAGCGGTGGTAACGGCCGACAGCGGT
GATGACGAGAGCGGTGGCCACAGTGTCTCCGACACCGAGTCGACGCTCGC
GCGGTTCCGCGCGCTGCTCAGGCTCTACGTCGGGACTCACAGCTTTCACA
ATTTTGTCACAGTTAAAAAGGCCCGGAAGGACAGGGAACGCGGCCGGGAA
GAGCGCGCGGCGGCGATGGCGGCGACGGCGGCGGCTGCGGCGGTCACCGG
GTCCGATGCAGCTGCGGTGGTTGCTGGCATTGCAATAGCCGTCGGAGAAG
ACTTGGAAACCGACGACGACGTAGAGTTTGTGGACGATGTCGAGGTAGAC
GGTTTTGTCGATGACGACGACGACGGCGGC
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