mRNA_P-wetherbeei_contig13070.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig13070.1.1
Unique NamemRNA_P-wetherbeei_contig13070.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: A0A836CB83_9STRA (GlcNAc-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CB83_9STRA)

HSP 1 Score: 210 bits (534), Expect = 7.650e-63
Identity = 100/170 (58.82%), Postives = 122/170 (71.76%), Query Frame = 1
Query:    1 QVRNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQGWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGTVDFVP 510
            QVRNE AKALRLMT PKLTTTW AG SF KCH  RAV YDP+LPQ+FDGEEFS FARL+T GYDVYTP+++Y+ HDYN  N P+A GW  +  RD AAE   S+ RLWTLL MP G + P   + V++G +GLG  R+L+QL ++  +DL  KT+    AD C  V +VP
Sbjct:  204 QVRNEGAKALRLMTQPKLTTTWAAGLSFSKCHSMRAVPYDPYLPQVFDGEEFSYFARLFTNGYDVYTPTKTYIGHDYNGANNPNAHGWYVATGRDRAAEEKMSDMRLWTLLEMPGGDSTPEGRQAVQFGPFGLGTARTLDQLMDFAMIDLRTKTHKADPADMCQDVHYVP 373          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: A0A6H5L6U7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L6U7_9PHAE)

HSP 1 Score: 179 bits (455), Expect = 2.690e-50
Identity = 89/177 (50.28%), Postives = 118/177 (66.67%), Query Frame = 1
Query:    1 QVRNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQGWRASRQRD-----EAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGT-VDFVPF 513
            Q RN+QA+A+  MT PKLTTTWGAG+SF KCH ER V YDP L +IFDGEEFSK ARLWT GYDVYTP R ++ HDY+H +      WR++++ D     +   LA  N RLW L+ MP  + +     +V+   WGLG +R+L++  E+ GVDL  K + G   DRCG+ V++VP+
Sbjct:  242 QPRNQQARAIHEMTEPKLTTTWGAGYSFSKCHAERRVPYDPNLDRIFDGEEFSKMARLWTNGYDVYTPRRGHLVHDYSHPHHEQTTSWRSNKKVDGDDGKDRQRLA--NQRLWNLMEMPLSSEEGR--REVQGDPWGLGDRRTLDEFIEFTGVDLRNKEHIGVTGDRCGSGVEWVPY 414          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: F0Y7S9_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y7S9_AURAN)

HSP 1 Score: 168 bits (425), Expect = 3.370e-46
Identity = 87/177 (49.15%), Postives = 110/177 (62.15%), Query Frame = 1
Query:    4 VRNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQ-------GWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGTVDFVPF 513
            VRN QAKA RL+  PKLTT W AG SF +CH ERAV YDP  P IF GEEFS+ AR +T GYD+YTP R+ V HDY H     +        G R ++ +   A+  A+N R+WTLLGMP G  DPA     R G +GLG KRSL+ L  + G++L  +T      +RCG +D+VP+
Sbjct:  233 VRNMQAKAARLLEKPKLTTLWAAGLSFSRCHAERAVPYDPHTPYIFWGEEFSRTARFFTNGYDIYTPPRTIVAHDYKHTQGDPSHFKWNGKGGPRLNQNKTIVAQRDAANRRIWTLLGMPGGDPDPAARR--RLGAYGLGDKRSLDDLVAFTGINLYNRTI---GPNRCGNIDWVPW 404          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: A0A8J2SM54_9STRA (Hypothetical protein n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SM54_9STRA)

HSP 1 Score: 167 bits (424), Expect = 3.680e-46
Identity = 84/178 (47.19%), Postives = 110/178 (61.80%), Query Frame = 1
Query:    4 VRNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQ-------GWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGTVDFVPFE 516
            VRN QAKA RL+  PKLTT W AG SF +CH ER V YDP+ P IF GEEFS+ AR +T GYD+YTP ++ + HDY H              G R +R +       A+N R+WTLLGMP G  DP++V  +  G +GLG KRSL+ L  + G++L  +T  G    RCG +D+VP++
Sbjct:  243 VRNMQAKAARLLKKPKLTTLWAAGLSFSRCHAERTVPYDPYTPYIFWGEEFSRTARFFTHGYDIYTPPKTLIAHDYKHTQGDPTHFKWNGRGGPRLNRNQTIIKARDAANKRIWTLLGMPGG--DPSMVSSL--GKYGLGTKRSLDDLIAFTGINLRNRTVSGSGNSRCGNIDWVPWD 416          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: I2CPM0_NANGC (Uncharacterized protein (Fragment) n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=I2CPM0_NANGC)

HSP 1 Score: 160 bits (405), Expect = 9.970e-46
Identity = 88/172 (51.16%), Postives = 108/172 (62.79%), Query Frame = 1
Query:    4 VRNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQGWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRY-GTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGTVDFVPFE 516
            VRNEQAK++ ++T PK+T+TW AGF+F KCH ++   YDP LP +FDGEEFSKFARLWTRGYDVYTP RS V+HDYNH  +  A    AS  RD   EL  S  RL  LLG           E   +   W LG +RSLEQL ++ GVD   +    H +  CG + +VPFE
Sbjct:   54 VRNEQAKSVMMLTRPKMTSTWAAGFAFNKCHADKRTPYDPHLPSLFDGEEFSKFARLWTRGYDVYTPHRSVVYHDYNHGPSTVAA---ASWTRD-GKELERSRNRLKVLLGDSTAPLARNTSEAHAFLARWDLGNRRSLEQLIQFTGVDTVNREIL-HSS--CGYLAWVPFE 218          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: A0A7S3UP91_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UP91_HETAK)

HSP 1 Score: 157 bits (398), Expect = 1.030e-45
Identity = 82/170 (48.24%), Postives = 104/170 (61.18%), Query Frame = 1
Query:    7 RNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQGWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGTVDFVPFE 516
            RN  A +   ++ PKL + W AGFSF +CHGER V YDP LP IFDGEEFS+ ARLWTRGYD YTP R  V HDYN     + QGWR + Q+++  + A S  R++ LL MP G +          G +GLG +RSL Q   +VGV L R+       + CGT+ +VPFE
Sbjct:    7 RNGDAFSAFHLSRPKLGSLWAAGFSFMRCHGERQVPYDPQLPNIFDGEEFSRSARLWTRGYDFYTPHRPVVVHDYNKPKDYNPQGWRNNNQQNKEKDKARSRQRIFDLLEMPEGTS----AGKAALGKYGLGDRRSLAQYEAFVGVSLKRREML---ENHCGTLQWVPFE 169          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: A0A7S1Z148_TRICV (Hypothetical protein (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1Z148_TRICV)

HSP 1 Score: 157 bits (397), Expect = 7.560e-43
Identity = 83/172 (48.26%), Postives = 105/172 (61.05%), Query Frame = 1
Query:    4 VRNEQAKALRLMTAPKLTTT-WGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHL-NAPHAQGWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGTVDFVPF 513
            VR    K  + ++ PKLT   WGAG SF KCH E  V  DP  P +FDGEEF++ AR WT GYD+YTP+R YV HDY    + P    W   R  D  A  AAS+ RL T+L MP G TDPAV + +R   +GLG +R+LEQL E+ GVDL  + +     +RCG + +VPF
Sbjct:  203 VRTHATKCAQNLSKPKLTNAIWGAGLSFSKCHAELKVPVDPHTPYVFDGEEFNRAARFWTYGYDIYTPNRVYVLHDYQRSQHNPVTASWH--RNTDMVA-TAASHKRLKTMLDMPRGETDPAVADRLRRSKFGLGDRRNLEQLMEFSGVDLRNEKFPIDGKNRCGNLQWVPF 371          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: A0A7S3UUL0_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UUL0_HETAK)

HSP 1 Score: 155 bits (391), Expect = 1.350e-42
Identity = 85/172 (49.42%), Postives = 110/172 (63.95%), Query Frame = 1
Query:    7 RNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAP-HAQGWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDL-SRKTYYGHQADRCGTVDFVPFE 516
            RNEQAKA R ++ PKLTT W AG SF KCHGER   YDP LP IFDGEE++K  RL+T GYD+YTP RSYV+HDY+H      A  W  SR+      + AS  R+ TLL M  G  D +     + G +GLG KR+++Q   + GVD+ +RK++    A+ CG V +VPF+
Sbjct:   25 RNEQAKACRTLSEPKLTTVWAAGLSFLKCHGERLTPYDPNLPGIFDGEEYTKGIRLFTHGYDMYTPHRSYVYHDYSHAKTDGRAHTW--SRKT-----VKASQERIMTLLEMNEG--DKSAEAKAKLGKYGLGPKRTIDQYINFTGVDIRNRKSF----ANACGNVAWVPFQ 183          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: D7FQK5_ECTSI ([Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase, family GT60 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQK5_ECTSI)

HSP 1 Score: 150 bits (379), Expect = 2.180e-40
Identity = 75/153 (49.02%), Postives = 101/153 (66.01%), Query Frame = 1
Query:    1 QVRNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQGWRASRQRD-----EAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGV 444
            Q RN+QA  +  MT PKLTTTWGAG+SF KCH ER V YDP L +IFDGEEFSK ARLWT GYDVYTP R ++ HDY+H +      WR++++ +     +   LA  N RLW L+ MP  + + +  ++V+   WGLG +R+L++  E+ G 
Sbjct:  284 QPRNQQA--IHEMTEPKLTTTWGAGYSFSKCHAERRVPYDPNLDRIFDGEEFSKMARLWTNGYDVYTPRRGHLVHDYSHPHHAQTTSWRSNKKVEGDGGKDRQRLA--NQRLWNLMEMPRSSKEGS--KEVQGDPWGLGDRRTLDEFIEFTGT 430          
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Match: A0A6H5KPV9_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KPV9_9PHAE)

HSP 1 Score: 150 bits (379), Expect = 9.110e-40
Identity = 80/171 (46.78%), Postives = 102/171 (59.65%), Query Frame = 1
Query:    7 RNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGEEFSKFARLWTRGYDVYTPSRSYVFHDYNHLN-APHAQGWRASRQRDEAAELAASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDLSRKTYYGHQADRCGTVDFVPFE 516
            R  QAK    + +PKLT TW AG S  KCH  +A  YDP L ++FDGEEFS  ARLWTRGYD YTP +S V HDYN +   P    W  ++    A +LA    R++TL GMP       + E +  G +GLG KR+LEQ  E++GV+L+     G    RCGT+ +VPFE
Sbjct:  244 RYVQAKLATKLPSPKLTNTWAAGLSLAKCHAWKASPYDPGLRKVFDGEEFSMHARLWTRGYDTYTPDKSLVGHDYNKVKEGPQPSSWLNNKMVAGAKDLAYK--RMFTLFGMPGKVDTEEIWESI--GQYGLGTKRTLEQYQEFIGVNLTTLEIMG---SRCGTLTYVPFE 407          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig13070.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CB83_9STRA7.650e-6358.82GlcNAc-domain-containing protein n=1 Tax=Tribonema... [more]
A0A6H5L6U7_9PHAE2.690e-5050.28Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
F0Y7S9_AURAN3.370e-4649.15Uncharacterized protein n=1 Tax=Aureococcus anopha... [more]
A0A8J2SM54_9STRA3.680e-4647.19Hypothetical protein n=2 Tax=Pelagomonas calceolat... [more]
I2CPM0_NANGC9.970e-4651.16Uncharacterized protein (Fragment) n=1 Tax=Nannoch... [more]
A0A7S3UP91_HETAK1.030e-4548.24Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S1Z148_TRICV7.560e-4348.26Hypothetical protein (Fragment) n=1 Tax=Trieres ch... [more]
A0A7S3UUL0_HETAK1.350e-4249.42Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
D7FQK5_ECTSI2.180e-4049.02[Skp1-protein]-hydroxyproline N-acetylglucosaminyl... [more]
A0A6H5KPV9_9PHAE9.110e-4046.78Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig13070contigP-wetherbeei_contig13070:89..1041 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog44056.XP_009036501.1
PFAMsGlcNAc
Model size516
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons2
Evalue5.52e-47
EggNOG OGs2CXJN@1|root,2RY14@2759|Eukaryota
DescriptionGlycosyltransferase (GlcNAc)
Cds size516
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185972.9376888-CDS-P-wetherbeei_contig13070:88..4681683185972.9376888-CDS-P-wetherbeei_contig13070:88..468Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig13070 89..468 +
1683185972.9468281-CDS-P-wetherbeei_contig13070:905..10411683185972.9468281-CDS-P-wetherbeei_contig13070:905..1041Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig13070 906..1041 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig13070.1.1prot_P-wetherbeei_contig13070.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig13070 89..1041 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig13070.1.1

>prot_P-wetherbeei_contig13070.1.1 ID=prot_P-wetherbeei_contig13070.1.1|Name=mRNA_P-wetherbeei_contig13070.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=172bp
QVRNEQAKALRLMTAPKLTTTWGAGFSFGKCHGERAVGYDPFLPQIFDGE
EFSKFARLWTRGYDVYTPSRSYVFHDYNHLNAPHAQGWRASRQRDEAAEL
AASNARLWTLLGMPNGATDPAVVEDVRYGTWGLGRKRSLEQLYEYVGVDL
SRKTYYGHQADRCGTVDFVPFE
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mRNA from alignment at P-wetherbeei_contig13070:89..1041+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig13070.1.1 ID=mRNA_P-wetherbeei_contig13070.1.1|Name=mRNA_P-wetherbeei_contig13070.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=953bp|location=Sequence derived from alignment at P-wetherbeei_contig13070:89..1041+ (Phaeothamnion wetherbeei SAG_119_79)
CAGGTGCGGAACGAGCAGGCAAAGGCGCTGCGACTCATGACGGCCCCCAA GCTGACCACGACGTGGGGCGCGGGCTTCTCCTTCGGCAAGTGCCACGGCG AGCGCGCCGTCGGCTACGACCCATTTTTGCCGCAAATCTTCGATGGCGAG GAGTTCTCGAAGTTCGCACGGCTCTGGACGCGCGGATACGACGTCTACAC ACCCAGCCGCAGCTACGTGTTTCACGATTACAACCACTTGAACGCGCCGC ATGCGCAGGGGTGGCGGGCGAGCCGGCAGCGCGACGAGGCGGCCGAACTG GCGGCATCCAACGCGCGGCTGTGGACGCTGCTGGGCATGCCGAACGGGGC GACGGATCCGGCGGTGGTGGAGGACGTCCGGTGAGTCCGAGGGAGAGAGG AGCGAGGGACGTTTTCCGTGTGCGGCTTCTTCCCTCCTCTCTTTGCGTTC GGAAGCGGCTTTTTGGTGGCTTCGTTCTTTACTACACTGTCTTGTTGTTC TCTTCTTTCTTTTCTTCTTGCTTCTTGACAAGTGGCGCCTGAGATTCACA CCTCGTTAGCACGGTCTATGTGCTTGAGGTCAAAAGTTTTCAGATTTGAC ACTCCGCCTTGCAGCAAACCACTAGCCTGATCAGCTCATCATAGCAATAG ACTGGGAGCCGTTCTCCTTTTGGCAGTTATGAGGCGGCTCGTGAAGGCGC TTTGCATCAACCACCTATCATTACTAGCTGATATAGTCTAGCGACAAGAA AGGGTTTACAAGTTCATTTATTCTGCTTACCTGTATTCCTCTTCCATCTA CTTCTCACGTTGCTCAGATACGGGACCTGGGGGCTCGGAAGAAAGCGGTC GCTGGAGCAGCTCTATGAGTATGTCGGGGTGGACCTGTCCCGCAAGACAT ATTACGGACATCAGGCGGACCGCTGTGGGACGGTCGACTTCGTGCCGTTC GAA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig13070:89..1041+

>mRNA_P-wetherbeei_contig13070.1.1 ID=mRNA_P-wetherbeei_contig13070.1.1|Name=mRNA_P-wetherbeei_contig13070.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=516bp|location=Sequence derived from alignment at P-wetherbeei_contig13070:89..1041+ (Phaeothamnion wetherbeei SAG_119_79)
CAGGTGCGGAACGAGCAGGCAAAGGCGCTGCGACTCATGACGGCCCCCAA
GCTGACCACGACGTGGGGCGCGGGCTTCTCCTTCGGCAAGTGCCACGGCG
AGCGCGCCGTCGGCTACGACCCATTTTTGCCGCAAATCTTCGATGGCGAG
GAGTTCTCGAAGTTCGCACGGCTCTGGACGCGCGGATACGACGTCTACAC
ACCCAGCCGCAGCTACGTGTTTCACGATTACAACCACTTGAACGCGCCGC
ATGCGCAGGGGTGGCGGGCGAGCCGGCAGCGCGACGAGGCGGCCGAACTG
GCGGCATCCAACGCGCGGCTGTGGACGCTGCTGGGCATGCCGAACGGGGC
GACGGATCCGGCGGTGGTGGAGGACGTCCGATACGGGACCTGGGGGCTCG
GAAGAAAGCGGTCGCTGGAGCAGCTCTATGAGTATGTCGGGGTGGACCTG
TCCCGCAAGACATATTACGGACATCAGGCGGACCGCTGTGGGACGGTCGA
CTTCGTGCCGTTCGAA
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