mRNA_P-wetherbeei_contig12045.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig12045.1.1
Unique NamemRNA_P-wetherbeei_contig12045.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: D8LAY3_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LAY3_ECTSI)

HSP 1 Score: 118 bits (296), Expect = 2.220e-24
Identity = 119/163 (73.01%), Postives = 135/163 (82.82%), Query Frame = 2 
Query:   29 MAWRRIDVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEARTGTEGTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPDLTDNRGNRPGDHFDGDVPDERRAAIAVMMGAV 517
            MAWRRIDVH+A ERGD+ V+ RYLDAGGD E++D +Y A+P+HWA LRGR  A  +LL+RG D+E+RT T+GT XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXP   DNRG+RPGD FD +V D  R AI   +  V
Sbjct:    1 MAWRRIDVHRAVERGDLAVVKRYLDAGGDPEQKDEEYGAAPVHWAALRGRSDALSLLLERGGDVESRTSTDGTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPKAEDNRGDRPGDKFDPEVTDSNRRAIKKALARV 163
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A6H5JTU0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTU0_9PHAE)

HSP 1 Score: 117 bits (292), Expect = 8.120e-24
Identity = 120/163 (73.62%), Postives = 134/163 (82.21%), Query Frame = 2 
Query:   29 MAWRRIDVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEARTGTEGTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPDLTDNRGNRPGDHFDGDVPDERRAAIAVMMGAV 517
            MAWRRIDVH+A ERGD+ V+ RYLDAGGD EK+D  Y A+P+HWA LRGR  A  +LL+RG D+E+RT T+GT XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXP   DNRG+RPGD FD +V D  R AI   +  V
Sbjct:    1 MAWRRIDVHRAVERGDLAVVKRYLDAGGDPEKKDEKYGAAPVHWAALRGRSDALSLLLERGGDVESRTCTDGTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPKAEDNRGDRPGDKFDPEVTDSDRRAIKQALARV 163
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A835ZAM8_9STRA (Ankyrin repeat protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZAM8_9STRA)

HSP 1 Score: 87.0 bits (214), Expect = 7.970e-17
Identity = 37/68 (54.41%), Postives = 52/68 (76.47%), Query Frame = 2 
Query:   29 MAWRRIDVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEART 232
            MAW+R+D  +AA+RG+V +L +YLD+GG +  +D  Y A+PLHWACLRG+  A  +L++R A LEART
Sbjct:    1 MAWKRVDPFRAAQRGEVSMLRQYLDSGGSISAQDDKYLATPLHWACLRGKLDAVALLIERSAPLEART 68
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A0M8MZ53_9BASI (Palmitoyltransferase n=1 Tax=Malassezia pachydermatis TaxID=77020 RepID=A0A0M8MZ53_9BASI)

HSP 1 Score: 61.2 bits (147), Expect = 3.910e-6
Identity = 31/63 (49.21%), Postives = 37/63 (58.73%), Query Frame = 2 
Query:   47 DVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEARTG 235
            DVH  A+RGDV  L   LDAG DV  RDAD   +PLHWA +         LL+RGAD+  + G
Sbjct:    7 DVHTCAQRGDVPALEALLDAGVDVNLRDAD-GVTPLHWAAINAHVACCEWLLERGADVHCQGG 68
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A836C9J9_9STRA (Ankyrin repeat-containing domain protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C9J9_9STRA)

HSP 1 Score: 58.2 bits (139), Expect = 1.510e-5
Identity = 29/64 (45.31%), Postives = 40/64 (62.50%), Query Frame = 2 
Query:   44 IDVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEARTG 235
            + + +AA RGD+ ++ R+L AGGDV  RD D+  SPLH AC  G  + A +LLD  A +  R G
Sbjct:    9 VSIFEAARRGDLAMVERWLQAGGDVNVRD-DHDWSPLHIACEAGYYEIAELLLDYSAQVNVRGG 71
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A1F4PID2_9BACT (Uncharacterized protein n=1 Tax=candidate division TM6 bacterium RIFCSPHIGHO2_12_FULL_32_22 TaxID=1802375 RepID=A0A1F4PID2_9BACT)

HSP 1 Score: 58.5 bits (140), Expect = 2.200e-5
Identity = 30/67 (44.78%), Postives = 39/67 (58.21%), Query Frame = 2 
Query:   47 DVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEARTGTEGT 247
            D+ KAAE G++V +   LDAG DV      +  + L WA   G    AR+LLDRGAD++A   T GT
Sbjct:   24 DLLKAAENGNLVGVEAALDAGADVNNATEGWRLTALMWAATNGHTDIARLLLDRGADIDAVDVTGGT 90
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A382QYV1_9ZZZZ (Uncharacterized protein (Fragment) n=1 Tax=marine metagenome TaxID=408172 RepID=A0A382QYV1_9ZZZZ)

HSP 1 Score: 55.8 bits (133), Expect = 3.380e-5
Identity = 25/57 (43.86%), Postives = 37/57 (64.91%), Query Frame = 2 
Query:   59 AAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEAR 229
            AAE GDV  +  YL    +V+ RD  Y  +PLH+A  RGR++ A +L++ GAD+ A+
Sbjct:   26 AAEEGDVEAVKGYLAIDVNVDARDDVYEGTPLHFAAFRGRKEVAELLINEGADVNAK 82
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A7S4KW95_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4KW95_9EUKA)

HSP 1 Score: 56.2 bits (134), Expect = 4.050e-5
Identity = 25/67 (37.31%), Postives = 42/67 (62.69%), Query Frame = 2 
Query:   44 IDVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEARTGTEG 244
            +D+HKAA++GD++++++ L +   V  +   Y  +PLHWAC  G+ +A   L+ +GAD  A    EG
Sbjct:    3 LDLHKAAQKGDLLLMDQLLSSRKVVVDKRDPYGGTPLHWACQAGQVEAINWLVSKGADAHAIATYEG 69
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A382VSR7_9ZZZZ (Uncharacterized protein (Fragment) n=1 Tax=marine metagenome TaxID=408172 RepID=A0A382VSR7_9ZZZZ)

HSP 1 Score: 54.7 bits (130), Expect = 6.040e-5
Identity = 28/62 (45.16%), Postives = 36/62 (58.06%), Query Frame = 2 
Query:   50 VHKAAERGDVVVLNRYLDAGGDVEKRDADYHA---SPLHWACLRGREQAARILLDRGADLEA 226
            +HKA   GD   +   LDAG DV  R+AD      +PLHWA   G  + A +L+ RGADL+A
Sbjct:   21 LHKAVRAGDADRVRELLDAGADVNVRNADKQRLQYTPLHWAAHFGHLEIAELLISRGADLDA 82
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BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Match: A0A442MDY3_9HYPH (C-type cytochrome n=1 Tax=Mesorhizobium sp. TaxID=1871066 RepID=A0A442MDY3_9HYPH)

HSP 1 Score: 56.2 bits (134), Expect = 6.510e-5
Identity = 30/66 (45.45%), Postives = 43/66 (65.15%), Query Frame = 2 
Query:   50 VHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGREQAARILLDRGADLEARTGTEGT 247
            +H AA++GDV  +   LDAG +V + D    A+PLH+A  R    AAR+LL+RGAD++A +   GT
Sbjct:   23 IHDAAKKGDVAAIAAALDAGANVNESDG--LAAPLHYAVNRQHLDAARLLLERGADVKAVSKLGGT 86
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig12045.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
D8LAY3_ECTSI2.220e-2473.01Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JTU0_9PHAE8.120e-2473.62Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A835ZAM8_9STRA7.970e-1754.41Ankyrin repeat protein n=1 Tax=Tribonema minus Tax... [more]
A0A0M8MZ53_9BASI3.910e-649.21Palmitoyltransferase n=1 Tax=Malassezia pachyderma... [more]
A0A836C9J9_9STRA1.510e-545.31Ankyrin repeat-containing domain protein n=1 Tax=T... [more]
A0A1F4PID2_9BACT2.200e-544.78Uncharacterized protein n=1 Tax=candidate division... [more]
A0A382QYV1_9ZZZZ3.380e-543.86Uncharacterized protein (Fragment) n=1 Tax=marine ... [more]
A0A7S4KW95_9EUKA4.050e-537.31Hypothetical protein (Fragment) n=1 Tax=Paramoeba ... [more]
A0A382VSR7_9ZZZZ6.040e-545.16Uncharacterized protein (Fragment) n=1 Tax=marine ... [more]
A0A442MDY3_9HYPH6.510e-545.45C-type cytochrome n=1 Tax=Mesorhizobium sp. TaxID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig12045contigP-wetherbeei_contig12045:2..1786 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D8LAY3
PFAMsAnk,Ank_2,Ank_4,Ank_5,zf-MYND
Model size1785
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.21e-25
EggNOG OGsCOG0666@1|root,KOG4177@2759|Eukaryota
Descriptionspectrin binding
Cds size1593
COG categoryI
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185936.0600228-UTR-P-wetherbeei_contig12045:1..1651683185936.0600228-UTR-P-wetherbeei_contig12045:1..165Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig12045 2..165 -
1683185936.0752869-UTR-P-wetherbeei_contig12045:1758..17861683185936.0752869-UTR-P-wetherbeei_contig12045:1758..1786Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig12045 1759..1786 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185936.068043-CDS-P-wetherbeei_contig12045:165..17581683185936.068043-CDS-P-wetherbeei_contig12045:165..1758Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig12045 166..1758 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig12045.1.1prot_P-wetherbeei_contig12045.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig12045 166..1758 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig12045.1.1

>prot_P-wetherbeei_contig12045.1.1 ID=prot_P-wetherbeei_contig12045.1.1|Name=mRNA_P-wetherbeei_contig12045.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=531bp
MAWRRIDVHKAAERGDVVVLNRYLDAGGDVEKRDADYHASPLHWACLRGR
EQAARILLDRGADLEARTGTEGTPLTWACMGGNPDVVEFLLDRGADIHAV
TRGRGTPLHHAASYGHPAAVRTLLARGADPDLTDNRGNRPGDHFDGDVPD
ERRAAIAVMMGAVLSSSSTGGSPVGPVSGGGGGSGVKAAGMTAAGGRGRT
ATDGWATRSPSPTPRGSNAAAGIGSSGGINPFADAASGAGFAAAGQRRSA
DGSAGGSITQNSSSDSLVASAAFRAPPGGAAAGVASGAAAAALASLTAHD
SPARRSDSSTGKTTDEELECRRLNGSLGGAGDVGGGTALGGAGAAGGLGG
EMSRASGATYVPPEEWPLAANSGAGGAAGGAAAGMAGSAAAEAAALLAAA
PADADLEELRDAVEEADAAVRRLTEGLRQRDDALRTMLALREADTRELAR
CAGERDGRVARLLTALERRGAGLAGVLTQREVEAHRLGRELEARALQLRQ
KVRLFPPFYRSRRKSERVWYTFLGLAWFLA*
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mRNA from alignment at P-wetherbeei_contig12045:2..1786-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig12045.1.1 ID=mRNA_P-wetherbeei_contig12045.1.1|Name=mRNA_P-wetherbeei_contig12045.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1785bp|location=Sequence derived from alignment at P-wetherbeei_contig12045:2..1786- (Phaeothamnion wetherbeei SAG_119_79)
AGTAAGAAAAAGGGTGGGCCGGGCATCCATGGCCTGGCGTCGCATCGACG
TCCACAAGGCCGCCGAGCGGGGCGACGTGGTGGTTCTAAACCGCTACCTC
GACGCCGGCGGCGACGTCGAGAAACGCGATGCCGACTACCACGCATCGCC
GCTCCACTGGGCCTGCCTCCGCGGCCGCGAGCAGGCGGCACGCATCCTCC
TCGATCGCGGAGCAGATCTGGAGGCGCGCACGGGCACGGAGGGCACGCCG
CTGACGTGGGCCTGCATGGGCGGCAACCCAGATGTCGTCGAATTCCTGCT
CGACCGTGGCGCCGACATCCACGCCGTGACGCGGGGCCGCGGTACACCGT
TGCACCACGCCGCGTCCTATGGCCACCCGGCCGCCGTCCGGACCCTTTTG
GCACGCGGCGCCGACCCGGACCTGACGGACAACCGCGGGAACCGCCCCGG
AGACCACTTCGACGGTGATGTGCCCGACGAGCGCCGCGCCGCCATCGCCG
TCATGATGGGGGCGGTGCTGTCCTCCAGCTCCACCGGTGGCTCGCCGGTG
GGGCCGGTTTCTGGCGGCGGCGGCGGCAGCGGCGTAAAGGCGGCGGGAAT
GACAGCGGCAGGTGGCCGGGGAAGAACGGCGACGGACGGCTGGGCGACGC
GGTCGCCGTCGCCGACGCCCCGCGGATCAAACGCCGCCGCTGGCATTGGC
AGCAGCGGTGGCATCAACCCATTTGCGGACGCCGCCAGCGGGGCCGGCTT
TGCGGCGGCCGGGCAGCGGCGATCGGCCGACGGCAGCGCGGGTGGATCGA
TCACGCAAAACTCGTCGTCGGACTCTCTGGTGGCGTCGGCAGCCTTCCGG
GCCCCTCCCGGCGGTGCGGCGGCAGGGGTGGCGTCCGGGGCGGCGGCAGC
AGCGCTCGCATCGCTGACGGCGCATGACAGCCCGGCCCGCCGCAGCGACA
GCAGCACCGGAAAGACGACCGACGAAGAGCTCGAATGCCGCCGCCTGAAC
GGCAGCCTCGGCGGCGCCGGCGACGTCGGCGGTGGCACAGCTCTTGGCGG
TGCCGGCGCTGCCGGCGGCCTCGGCGGCGAGATGAGCAGAGCGTCTGGTG
CAACTTACGTACCTCCGGAGGAGTGGCCGTTGGCGGCCAACAGTGGAGCT
GGTGGGGCAGCTGGTGGGGCAGCGGCGGGGATGGCAGGGTCTGCAGCCGC
GGAGGCGGCGGCCCTGCTGGCGGCGGCGCCCGCCGACGCCGACCTCGAGG
AGCTCCGCGACGCGGTGGAGGAGGCCGACGCCGCCGTGCGCCGCCTGACG
GAGGGCCTCCGGCAGCGCGACGATGCGCTGCGGACGATGTTGGCACTGCG
CGAGGCAGACACCCGCGAACTGGCGCGCTGCGCCGGGGAGCGAGACGGAC
GGGTAGCGCGGCTGCTGACGGCGCTGGAGCGGCGCGGTGCCGGGCTGGCG
GGCGTGCTAACGCAACGGGAGGTGGAGGCGCACCGGCTCGGCCGGGAGCT
TGAGGCCCGTGCCCTGCAGCTCCGGCAAAAGGTGCGCCTGTTTCCCCCTT
TTTACCGGTCGCGGAGAAAGAGTGAACGCGTGTGGTATACCTTCCTTGGT
CTTGCTTGGTTTCTGGCGTAGGGTGCTATTTTGTTTTTGTGGTCAACTGG
GTATGCCGAAGCGTGATGCCATAGTTGTTTTCCAACTAAATTCCACATCT
CTAAAAACGGTACTTGGAACGAATGCACTATCGCTGCTGTAATGACAAGG
GTCACAAAAAGCCAGCACTGGACGGAGGGAAAGAC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig12045:2..1786-

>mRNA_P-wetherbeei_contig12045.1.1 ID=mRNA_P-wetherbeei_contig12045.1.1|Name=mRNA_P-wetherbeei_contig12045.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=1593bp|location=Sequence derived from alignment at P-wetherbeei_contig12045:2..1786- (Phaeothamnion wetherbeei SAG_119_79)
ATGGCCTGGCGTCGCATCGACGTCCACAAGGCCGCCGAGCGGGGCGACGT
GGTGGTTCTAAACCGCTACCTCGACGCCGGCGGCGACGTCGAGAAACGCG
ATGCCGACTACCACGCATCGCCGCTCCACTGGGCCTGCCTCCGCGGCCGC
GAGCAGGCGGCACGCATCCTCCTCGATCGCGGAGCAGATCTGGAGGCGCG
CACGGGCACGGAGGGCACGCCGCTGACGTGGGCCTGCATGGGCGGCAACC
CAGATGTCGTCGAATTCCTGCTCGACCGTGGCGCCGACATCCACGCCGTG
ACGCGGGGCCGCGGTACACCGTTGCACCACGCCGCGTCCTATGGCCACCC
GGCCGCCGTCCGGACCCTTTTGGCACGCGGCGCCGACCCGGACCTGACGG
ACAACCGCGGGAACCGCCCCGGAGACCACTTCGACGGTGATGTGCCCGAC
GAGCGCCGCGCCGCCATCGCCGTCATGATGGGGGCGGTGCTGTCCTCCAG
CTCCACCGGTGGCTCGCCGGTGGGGCCGGTTTCTGGCGGCGGCGGCGGCA
GCGGCGTAAAGGCGGCGGGAATGACAGCGGCAGGTGGCCGGGGAAGAACG
GCGACGGACGGCTGGGCGACGCGGTCGCCGTCGCCGACGCCCCGCGGATC
AAACGCCGCCGCTGGCATTGGCAGCAGCGGTGGCATCAACCCATTTGCGG
ACGCCGCCAGCGGGGCCGGCTTTGCGGCGGCCGGGCAGCGGCGATCGGCC
GACGGCAGCGCGGGTGGATCGATCACGCAAAACTCGTCGTCGGACTCTCT
GGTGGCGTCGGCAGCCTTCCGGGCCCCTCCCGGCGGTGCGGCGGCAGGGG
TGGCGTCCGGGGCGGCGGCAGCAGCGCTCGCATCGCTGACGGCGCATGAC
AGCCCGGCCCGCCGCAGCGACAGCAGCACCGGAAAGACGACCGACGAAGA
GCTCGAATGCCGCCGCCTGAACGGCAGCCTCGGCGGCGCCGGCGACGTCG
GCGGTGGCACAGCTCTTGGCGGTGCCGGCGCTGCCGGCGGCCTCGGCGGC
GAGATGAGCAGAGCGTCTGGTGCAACTTACGTACCTCCGGAGGAGTGGCC
GTTGGCGGCCAACAGTGGAGCTGGTGGGGCAGCTGGTGGGGCAGCGGCGG
GGATGGCAGGGTCTGCAGCCGCGGAGGCGGCGGCCCTGCTGGCGGCGGCG
CCCGCCGACGCCGACCTCGAGGAGCTCCGCGACGCGGTGGAGGAGGCCGA
CGCCGCCGTGCGCCGCCTGACGGAGGGCCTCCGGCAGCGCGACGATGCGC
TGCGGACGATGTTGGCACTGCGCGAGGCAGACACCCGCGAACTGGCGCGC
TGCGCCGGGGAGCGAGACGGACGGGTAGCGCGGCTGCTGACGGCGCTGGA
GCGGCGCGGTGCCGGGCTGGCGGGCGTGCTAACGCAACGGGAGGTGGAGG
CGCACCGGCTCGGCCGGGAGCTTGAGGCCCGTGCCCTGCAGCTCCGGCAA
AAGGTGCGCCTGTTTCCCCCTTTTTACCGGTCGCGGAGAAAGAGTGAACG
CGTGTGGTATACCTTCCTTGGTCTTGCTTGGTTTCTGGCGTAG
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