mRNA_P-wetherbeei_contig1129.4.4 (mRNA) Phaeothamnion wetherbeei SAG_119_79

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A0A835YLH7_9STRA (RmlC-like cupin domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLH7_9STRA)

HSP 1 Score: 409 bits (1050), Expect = 4.040e-135
Identity = 204/303 (67.33%), Postives = 233/303 (76.90%), Query Frame = 3

Query:  480 FKSRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMPGPEG-AHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEG-GVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGDGVTLRTLDEPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSEIGLPITHA 1382
            FKSRS D+VVLS AQ+EG GATVRRSIG  +LRNFDPFLMLDE     PAGFPDHPHRGFETV+YMLEG F+HEDF+G+RG IG GDLQWMTAGRGI+HSEMP  +G +HGLQLWVNL +KDKMCEPAYQELK E+IP   +G GV+A VIAG+ALG  SPVYTRTP  Y+H+ M+P S +  AIP GW+ FLYTL G V VG +G             + AHHT+TL  DG+GV ++     ADFVLI G+P  EPIVQHGPFVMNTR EI QAM DYQ+G NGFE A  WYS+IGLPITHA
Sbjct:    4 FKSRSADKVVLSRAQSEGVGATVRRSIGNHELRNFDPFLMLDEFDSGKPAGFPDHPHRGFETVTYMLEGAFEHEDFMGNRGKIGPGDLQWMTAGRGIVHSEMPFGDGRSHGLQLWVNLGSKDKMCEPAYQELKAEEIPEVSDGKGVSARVIAGTALGTDSPVYTRTPAHYVHYTMEPGSVLQHAIPAGWSAFLYTLKGDVEVGGSGGVE----------VAAHHTITLDGDGEGVEVKAGKAGADFVLICGKPHGEPIVQHGPFVMNTRAEIQQAMLDYQSGSNGFEGAGKWYSQIGLPITHA 296          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: D7FTF8_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FTF8_ECTSI)

HSP 1 Score: 406 bits (1043), Expect = 5.240e-134
Identity = 202/302 (66.89%), Postives = 231/302 (76.49%), Query Frame = 3

Query:  480 FKSRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMP-GPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGDGVTLRTLDEPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSEIGLPITHA 1382
            F SRSVDQVVLS  QAEG GA VRRSIGG  LRNFDPFLMLDE  VA  AGFPDHPHRGFETV+YML+G  +HEDF GHRG IG G LQWMTAG+GI+H+EMP GP+  HGLQLWVNL + DKM  PAYQEL  ++IP A + GVTA+VIAG ALG++SPV TRTPT YIHFKM+P S + Q IP   N FLY L G  AVG      + KD+     I AH+TVTLT+ G GV ++T D  ADFVL+ G+P NEP+VQHGPFVMNTR+EI +AM DYQ+  NGFENA  WYSEIGLP+THA
Sbjct:    7 FSSRSVDQVVLSREQAEGQGARVRRSIGGVALRNFDPFLMLDEFDVATTAGFPDHPHRGFETVTYMLKGIMEHEDFCGHRGKIGPGSLQWMTAGKGIVHAEMPTGPDRGHGLQLWVNLKSGDKMVSPAYQELNADEIPRATKDGVTAIVIAGEALGISSPVQTRTPTHYIHFKMEPGSELKQPIPTNMNGFLYILEGKAAVGPG---REEKDQ-----IQAHNTVTLTKGGSGVLVKTFDSSADFVLVCGEPTNEPVVQHGPFVMNTRQEIQEAMLDYQSSSNGFENASKWYSEIGLPMTHA 300          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: F2TX92_SALR5 (Pirin family protein n=1 Tax=Salpingoeca rosetta (strain ATCC 50818 / BSB-021) TaxID=946362 RepID=F2TX92_SALR5)

HSP 1 Score: 380 bits (975), Expect = 3.140e-123
Identity = 196/324 (60.49%), Postives = 232/324 (71.60%), Query Frame = 3

Query:  417 AAGSAAAVATCSVLSAA---STMPFKSRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMPGPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGD--GVTLRTLDEPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSEIGLPI 1373
            A G+  A A+CS  + +   S  P  SR+V+Q +LS  Q EG GA VRRSIG  +LRNFDPFLMLDE +V   AGFPDHPHRGFETV+YML+G F+HEDF GHRG I  GDLQWMTAGRG++H+EMP  E  HGLQLWVNL AKDK+ EP YQELK ++IP A + GV A++IAG A GV SPV+TRTPT Y+HFKMQP S +DQ IP   N FLYTL G + VGE+G   +           AH TVTLTRD +  GVT++     A+FVLI G P  EPIVQ+GPFVMNT E+I QA  DYQTG+NGFE AR + SEIG P+
Sbjct:   29 AVGAKPATASCSTTTRSIMTSAAPVVSRAVNQQLLSQEQGEGMGARVRRSIGRPELRNFDPFLMLDEFRVKKGAGFPDHPHRGFETVTYMLDGAFEHEDFCGHRGRINAGDLQWMTAGRGVVHAEMPASEEGHGLQLWVNLGAKDKLVEPQYQELKADEIPHAEKDGVEAIIIAGKAFGVESPVFTRTPTYYLHFKMQPDSRLDQEIPAEMNAFLYTLSGTILVGESGEKCE-----------AHSTVTLTRDDEQTGVTIKADKGAAEFVLIAGVPHGEPIVQYGPFVMNTPEQIQQAFLDYQTGRNGFEKARRFQSEIGKPL 341          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A0A226EWE3_FOLCA (Pirin n=1 Tax=Folsomia candida TaxID=158441 RepID=A0A226EWE3_FOLCA)

HSP 1 Score: 369 bits (947), Expect = 9.700e-120
Identity = 181/304 (59.54%), Postives = 221/304 (72.70%), Query Frame = 3

Query:  474 MPFKSRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYML---EGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMP-GPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGDGVTLRTLDEPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSEIGLPI 1373
            M F SR V QV+LSV Q+EG GA VRRSIG  +LRNFDPFLMLDE +   PAGFPDHPHRGFETV+Y+L   +G+F+HEDF GHRG IG GDLQWMTAGRGILHSEMP   E +HGLQLW+NL+AKDKMC P YQEL+ ED+P   + GVTA++IAG +LG  S +YTRTPT+YIHF M+P S ++Q +P  W  F YT+ G V    AG+  D           AH TVT    GDG+   T DEP +FVLI G+P+ EP+VQHGPFVM+T++EI++A+ DY   +NGFENA+ W SEI   +
Sbjct:    1 MSFSSRGVKQVLLSVEQSEGVGAKVRRSIGRQELRNFDPFLMLDEFRAGRPAGFPDHPHRGFETVTYILPTSKGSFEHEDFCGHRGTIGPGDLQWMTAGRGILHSEMPLSDEISHGLQLWINLSAKDKMCPPEYQELRSEDVPKITKDGVTAIIIAGESLGTVSKIYTRTPTNYIHFIMEPNSRIEQRVPSEWAAFAYTVEGKVKF-SAGNFID-----------AHSTVTFNPGGDGIIAETGDEPGNFVLISGKPIGEPVVQHGPFVMSTQKEIIEAINDYSQAKNGFENAQTWESEIAKKL 292          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A9V3G4_MONBE (Predicted protein n=1 Tax=Monosiga brevicollis TaxID=81824 RepID=A9V3G4_MONBE)

HSP 1 Score: 370 bits (950), Expect = 1.930e-119
Identity = 201/344 (58.43%), Postives = 239/344 (69.48%), Query Frame = 3

Query:  351 AATGLLHRVAAFCSILPSSSAGAAGSAAAVATCSVLSAASTMPFKSRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMPGPEG---AHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDG--DGVTLRTL-DEPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSEIG 1364
            A T     VAA     P++ A A GS +A   CS       M F SR+VD+ VLS  Q EGAGA VRRSIG A LRNFDPFLMLDE +V   AGFPDHPHRGFETV++ML+G F+HEDF GH G IG GDLQWMTAG+GI+H+EMP PE     HGLQLW+NL +KDKM  P YQELK EDIP   + GV   VIAG ALG  SPVYT TP+ Y+HFK+QP+S ++QAIP  +N F+YTL G + +G+ G+  D           AHHT TLTRD   +GV L    +E A+FVLIGG+P NEPI Q+GPFVMNTREEIM+A  DYQ+G+NGFE A  + S+IG
Sbjct:   10 AQTAFRFAVAAALCTAPATRAVAQGSCSASGICSE-PITRNMAFVSRAVDKKVLSREQGEGAGARVRRSIGNAALRNFDPFLMLDEFRVPLTAGFPDHPHRGFETVTFMLDGLFEHEDFAGHAGTIGPGDLQWMTAGKGIVHAEMPHPESDAEPHGLQLWINLGSKDKMVPPKYQELKNEDIPKVEKDGVWVAVIAGEALGTKSPVYTVTPSCYLHFKIQPQSRIEQAIPPEFNAFVYTLKGKLKIGDKGTEID-----------AHHTATLTRDASANGVVLANEGEEVAEFVLIGGEPHNEPIEQYGPFVMNTREEIMKAFTDYQSGRNGFERAPTFQSKIG 341          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A0A0D2X453_CAPO3 (Pirin-like protein n=3 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2X453_CAPO3)

HSP 1 Score: 368 bits (945), Expect = 3.030e-119
Identity = 192/307 (62.54%), Postives = 217/307 (70.68%), Query Frame = 3

Query:  456 LSAASTMPFK--SRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMPGPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRD--GDGVTLRTLDEPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSEIG 1364
            +SAA + P K  SR V + +L V Q EG GA VRRSIG  +L   DPFLMLD     PPAGFPDHPHRGFETVSY+LEG F HEDF GH+G +  GDLQWMTAGRGI+HSE+P  +   GLQLWVNLA KDKM EP YQEL K+DIPVA + GVT  VIAGS++ + S VYTRTPT Y+ FK+ P SS +QAIP GWN F +T+ G    G A SA + K           HTV L  D   DGVT  T DEPA FVLI G+PLNEPIVQHGPFVMNTREEIMQ +RDYQ  +NGFE A GWYSE G
Sbjct:    1 MSAALSTPIKFLSRRVVKSILGVEQGEGVGARVRRSIGRPELGRLDPFLMLDYFTGVPPAGFPDHPHRGFETVSYLLEGKFAHEDFAGHKGVLNAGDLQWMTAGRGIVHSEVPLTKSV-GLQLWVNLARKDKMIEPNYQELLKKDIPVATKDGVTVTVIAGSSMDIDSAVYTRTPTMYLDFKLDPNSSFNQAIPKGWNGFAFTISGKAYFGPADSAVEEKP---------FHTVLLDNDESADGVTFSTKDEPAHFVLIAGKPLNEPIVQHGPFVMNTREEIMQTIRDYQERKNGFERAAGWYSEAG 297          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A0A1Y1HPA7_KLENI (Cupin-domain containing protein n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1HPA7_KLENI)

HSP 1 Score: 352 bits (904), Expect = 1.930e-113
Identity = 175/292 (59.93%), Postives = 208/292 (71.23%), Query Frame = 3

Query:  483 KSRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMPGPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGDGVTLRTLD-EPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYS 1355
            K R + + +LS  Q+EG GA VRRSIG  +LRN DPFLMLDE  V PPAGFPDHPHRG ETV+YML+GTF H+DF GH+G I  GD+QWMTAGRGI+HSE+P  + AHGLQLWVNLAAKDKM EPAYQEL   +IP A + GV+  VIAG +LG++SPV+TRTP+ Y+ F +QP +S+ Q +P GWN F YTL G     +  S             G HHT+ L   GDG+ +     EPA FVLI G+PLNEP+VQHGPFVMNTREEIMQ   DYQ G+NGFE A  W S
Sbjct:    8 KPRKIIKKLLSREQSEGDGARVRRSIGRPELRNLDPFLMLDEFSVQPPAGFPDHPHRGQETVTYMLKGTFTHQDFTGHKGTINPGDVQWMTAGRGIVHSEIPASDDAHGLQLWVNLAAKDKMVEPAYQELLASEIPKAEKDGVSVAVIAGESLGISSPVFTRTPSMYLDFTLQPGASISQPVPEGWNGFAYTLNGTAVFDDVES-------------GPHHTLQLG-PGDGIKVENKGAEPARFVLIAGKPLNEPVVQHGPFVMNTREEIMQTFNDYQYGRNGFERAHTWVS 285          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A0A1D1UQH4_RAMVA (Uncharacterized protein n=1 Tax=Ramazzottius varieornatus TaxID=947166 RepID=A0A1D1UQH4_RAMVA)

HSP 1 Score: 351 bits (900), Expect = 1.280e-112
Identity = 172/294 (58.50%), Postives = 207/294 (70.41%), Query Frame = 3

Query:  483 KSRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMPGPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGDGVTLRTL--DEPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSE 1358
            ++RSVD++VLSV QAEG GA VRRSIG ++L   DPFL+LD   V PP GFPDHPHRGFETV+YM+ G+F HEDF  H+G IG GDLQWMTAGRG+LHSE+PGPEG  GLQLWVNLAAKDKM    YQEL+K  IPVA + GVTA VIAG  LG+ SPV TRTPT Y+   ++P + + Q +P  W  F+Y   G    G  G   D K   +       +TVT  ++GD + +     D P+  +LI GQPLNEPI +HGPFVMNT+EEI QA+RDYQ G+NGFENA  W SE
Sbjct:    7 QARSVDKMVLSVEQAEGRGARVRRSIGKSELSTLDPFLLLDHFNVKPPGGFPDHPHRGFETVTYMINGSFTHEDFKKHKGTIGPGDLQWMTAGRGVLHSEIPGPEGGEGLQLWVNLAAKDKMVPADYQELRKPSIPVAKQNGVTATVIAGECLGIKSPVKTRTPTMYLDLTLEPGAKLSQPVPSDWTAFVYIWSGKFTFGPEG---DQKTNEVF------YTVTFKKNGDSIFMANALEDCPSRLILIAGQPLNEPIARHGPFVMNTQEEISQAIRDYQAGENGFENAHSWQSE 291          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A0A6J8ANT6_MYTCO (PIR n=3 Tax=Mytilus TaxID=6548 RepID=A0A6J8ANT6_MYTCO)

HSP 1 Score: 345 bits (886), Expect = 1.130e-110
Identity = 174/293 (59.39%), Postives = 210/293 (71.67%), Query Frame = 3

Query:  486 SRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMP-GPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGDGVTLRTLD-EPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSE 1358
            SR+V Q + +  Q EG GA VRRS+G ++LRNFDPFL+LD+ +V  PAGFPDHPHRGFETV+Y+LEG   HEDF GH+G I  GDLQWMTAGRGI+H EMP G E AHGLQLWVNL++KDKM  PAYQEL  +DIP   + GV   VIAG +LG+ SPVYTRTPT Y+ F++   + ++Q IP GWN F+YTL G    GE G AA           GAH+T+ L ++GDGV +     E   FVLIGG+PL EP+ QHGPFVMNT +EI QAM DYQ G+NGFENA  W S+
Sbjct:    3 SRNVKQAIQNKEQDEGVGAKVRRSVGSSQLRNFDPFLLLDDFRVKAPAGFPDHPHRGFETVTYVLEGGVTHEDFCGHKGTIHPGDLQWMTAGRGIVHCEMPHGTEQAHGLQLWVNLSSKDKMTAPAYQELLDKDIPKTKQNGVHVKVIAGESLGIKSPVYTRTPTMYLDFQLDQGAGLNQPIPQGWNVFIYTLSGKATFGE-GEAAKQH--------GAHYTLVLDQNGDGVRVENKSPELCRFVLIGGKPLGEPVKQHGPFVMNTPDEIQQAMFDYQMGKNGFENASKWESD 286          

BLAST of mRNA_P-wetherbeei_contig1129.4.4 vs. uniprot
Match: A0A8B6FFM8_MYTGA (Uncharacterized protein n=2 Tax=Mytilus galloprovincialis TaxID=29158 RepID=A0A8B6FFM8_MYTGA)

HSP 1 Score: 345 bits (885), Expect = 1.590e-110
Identity = 175/293 (59.73%), Postives = 209/293 (71.33%), Query Frame = 3

Query:  486 SRSVDQVVLSVAQAEGAGATVRRSIGGAKLRNFDPFLMLDEAKVAPPAGFPDHPHRGFETVSYMLEGTFKHEDFVGHRGDIGKGDLQWMTAGRGILHSEMP-GPEGAHGLQLWVNLAAKDKMCEPAYQELKKEDIPVAVEGGVTAVVIAGSALGVTSPVYTRTPTSYIHFKMQPRSSVDQAIPVGWNCFLYTLGGAVAVGEAGSAADTKDEPILEPIGAHHTVTLTRDGDGVTLRTLD-EPADFVLIGGQPLNEPIVQHGPFVMNTREEIMQAMRDYQTGQNGFENARGWYSE 1358
            SR V Q + +  Q EG GA VRRS+G  +LRNFDPFL+LD+  V  PAGFPDHPHRGFETV+Y+LEG   HEDF GH+G I  GDLQWMTAGRGI+H EMP G E AHGLQLWVNLA+KDKM  PAYQEL  +DIP   + GV   VIAG +LG+ SPVYTRTPT Y+ F++   ++++Q IP GWN F+YTL G    GE GSAA   +        AH+T+ L ++GDG+ +     E   FVLIGG+PL EP+ QHGPFVMNT EEI QAM DYQ G+NGFENA  W S+
Sbjct:    3 SRKVKQAIQNKEQDEGVGAKVRRSVGSNQLRNFDPFLLLDDFSVKAPAGFPDHPHRGFETVTYVLEGGVTHEDFCGHKGTIHPGDLQWMTAGRGIVHCEMPHGTEQAHGLQLWVNLASKDKMTAPAYQELLDKDIPKTKQNGVHVKVIAGESLGIKSPVYTRTPTMYLDFQLDQGAALNQPIPQGWNSFIYTLSGKATFGE-GSAAKQHE--------AHNTLVLDQNGDGIRVENKSPELCRFVLIGGKPLGEPVKQHGPFVMNTPEEIQQAMFDYQMGKNGFENASRWESD 286