mRNA_P-wetherbeei_contig10933.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig10933.1.1
Unique NamemRNA_P-wetherbeei_contig10933.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: A0A7X9AFF1_9BURK (Ribosomal silencing factor RsfS n=1 Tax=Burkholderiales bacterium TaxID=1891238 RepID=A0A7X9AFF1_9BURK)

HSP 1 Score: 145 bits (367), Expect = 9.220e-43
Identity = 64/111 (57.66%), Postives = 87/111 (78.38%), Query Frame = 1
Query:    7 LESLEMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVH 339
            +E+LE+AR  A++ D+K+  D++V DL ++SI+CDYFV+ SAPTR+QT+ +AR I+E L   G   K  QG  EG+WILMDYGVIVVH+F+  EREFYDLEGLWR+A +V+
Sbjct:    7 VETLELARRIADLCDEKMTNDVVVLDLQNVSILCDYFVLASAPTRIQTRKVARDIDEMLSAEGLQPKSIQGMPEGAWILMDYGVIVVHLFMDREREFYDLEGLWREAAVVY 117          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: A0A7Z9UD74_9BACT (Ribosomal silencing factor RsfS n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7Z9UD74_9BACT)

HSP 1 Score: 137 bits (344), Expect = 2.070e-39
Identity = 61/110 (55.45%), Postives = 83/110 (75.45%), Query Frame = 1
Query:    7 LESLEMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLV 336
            +ESLE+A+ AA+ A +    D+++ D+ D++ VCDYFVI SAPTR+QTKDIAR +E+ L+  G A  R QG  E SWIL+DYG++V+H+FL  EREFYDLEG W DA ++
Sbjct:    1 MESLEIAKIAAQTAHEVKADDVLILDVRDMTTVCDYFVIASAPTRIQTKDIARKMEQALEIEGVAKSRIQGRDEASWILLDYGMVVLHVFLTQEREFYDLEGRWSDAEII 110          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: F6B8Q1_DESCC (Ribosomal silencing factor RsfS n=1 Tax=Desulfotomaculum nigrificans (strain DSM 14880 / VKM B-2319 / CO-1-SRB) TaxID=868595 RepID=F6B8Q1_DESCC)

HSP 1 Score: 121 bits (303), Expect = 3.550e-33
Identity = 55/116 (47.41%), Postives = 80/116 (68.97%), Query Frame = 1
Query:    7 LESLEMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVHMDFQA 354
            L   E+   A + ADDK  +DI V D++ +SI+CDYF+I +  +R Q + +A  IEE+L+ AG    R +GFREG+WIL+DYG +VVH+F + ER+FY+LE LW DA +V +  +A
Sbjct:    3 LSPKEIVDIAHQAADDKKAKDITVLDISHISIICDYFLICTGGSRTQVQSVAEHIEEKLEEAGVKPLRKEGFREGTWILLDYGDVVVHVFQEMERDFYNLERLWGDAQVVEISAKA 118          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: A0A355TP36_9BACT (Ribosomal silencing factor RsfS n=1 Tax=bacterium UBP9_UBA11836 TaxID=2060920 RepID=A0A355TP36_9BACT)

HSP 1 Score: 121 bits (304), Expect = 4.640e-33
Identity = 53/109 (48.62%), Postives = 77/109 (70.64%), Query Frame = 1
Query:   13 SLEMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVH 339
            SL+MA+A A+V   KLG+D +V D++  S++CDYFVI  APTR Q +DIA +I++ L   G   K  QG  + +W LMDY  ++VH+F+  ER FY+LEG+W+ AP+V+
Sbjct:   18 SLDMAKAIADVCYQKLGKDTVVLDMSKDSVLCDYFVICDAPTRNQLRDIAENIDKMLSEQGIEPKSIQGKNDNAWTLMDYSSVIVHLFISGERSFYNLEGMWKSAPIVY 126          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: A0A2N2A2H8_9FIRM (Ribosomal silencing factor RsfS n=1 Tax=Firmicutes bacterium HGW-Firmicutes-8 TaxID=2013789 RepID=A0A2N2A2H8_9FIRM)

HSP 1 Score: 117 bits (293), Expect = 1.040e-31
Identity = 49/114 (42.98%), Postives = 78/114 (68.42%), Query Frame = 1
Query:    1 ILLESLEMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVHM 342
            ++L   ++A   A  A+DK  +D+++ D++D+S+VCDYFVI S  +  Q K IA ++E +L+  G    R +GF++G W+L+DYG +V HIF + +R FY+LE LW DA +VH+
Sbjct:    1 MILSPDKVASLVAAAAEDKKAKDVLILDISDVSVVCDYFVICSGLSTTQVKAIAENVETKLEEQGMKKLRVEGFKDGHWVLLDYGSVVAHIFQEDDRHFYNLEHLWGDARVVHL 114          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: A0A2N2DKM5_9FIRM (Ribosomal silencing factor RsfS n=1 Tax=Firmicutes bacterium HGW-Firmicutes-14 TaxID=2013775 RepID=A0A2N2DKM5_9FIRM)

HSP 1 Score: 117 bits (293), Expect = 1.040e-31
Identity = 51/108 (47.22%), Postives = 76/108 (70.37%), Query Frame = 1
Query:   19 EMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVHM 342
            ++A  AA  A++K  +D+I+ D+ D+S VCDYFVI S  +  Q K IA +IE++LK  G    R +GF++  W+L+DYG +VVH+F + +REFY+LE LW DA +VH+
Sbjct:    7 KVAAGAARAAEEKKAKDVILLDIHDISTVCDYFVICSGMSSTQVKAIAENIEKKLKEHGVMKLRMEGFKDAHWVLLDYGSVVVHVFREDDREFYNLEHLWGDARVVHL 114          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: UPI001F50C2B0 (ribosome silencing factor n=1 Tax=Acidibacillus sp. S0AB TaxID=2005001 RepID=UPI001F50C2B0)

HSP 1 Score: 117 bits (293), Expect = 1.100e-31
Identity = 51/108 (47.22%), Postives = 76/108 (70.37%), Query Frame = 1
Query:   19 EMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVHM 342
            E A+ AA+ A+DK   D+++ D+ +LSI+ DYFVI SA +R Q + IA SIEE ++  G   + T+G R+G W+L+D+G ++VH+F + ER F+DLE LW DAP + M
Sbjct:    7 EYAKLAADAAEDKKANDVVILDIRELSIIADYFVICSANSRTQAQAIADSIEEHMELRGVKMRGTEGHRDGKWVLLDFGDVIVHVFQEEERRFFDLERLWGDAPRLSM 114          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: UPI001957FF84 (ribosome silencing factor n=1 Tax=Desulforadius tongensis TaxID=1216062 RepID=UPI001957FF84)

HSP 1 Score: 116 bits (291), Expect = 2.360e-31
Identity = 50/107 (46.73%), Postives = 74/107 (69.16%), Query Frame = 1
Query:   19 EMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVH 339
            +M   A   A++K   DI+V D++ +S++CDYF+I SA +  Q K IA +I++++   G A K  +G REG+W+L+DYG +VVHIF + ER FY+LE LW DA +VH
Sbjct:    7 QMVDEACRAAEEKKAHDIVVLDISGVSVICDYFIICSANSTTQVKAIAENIQDKMARQGVALKHREGLREGNWVLLDYGDVVVHIFREKERHFYNLERLWGDAEVVH 113          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: A0A357VG05_9FIRM (Ribosomal silencing factor RsfS n=3 Tax=Clostridia TaxID=186801 RepID=A0A357VG05_9FIRM)

HSP 1 Score: 115 bits (289), Expect = 4.340e-31
Identity = 48/114 (42.11%), Postives = 78/114 (68.42%), Query Frame = 1
Query:    4 LLESLEMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVHMD 345
            + ++L +AR AA+ A DK GR++++ D+  +S++ DYFVIT   +  Q + I+R IE+++  AG    R +G+    W+L+D+G +VVH+FL+ +R FYDLE LW DA LV ++
Sbjct:    1 MTDALRVARVAAQAAKDKRGREVVILDIRKISLIADYFVITHGTSTTQVQAISREIEDKVAEAGGRLLRREGYTSARWVLLDFGGVVVHVFLEEDRRFYDLERLWADAELVELE 114          
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Match: K8DYK1_9FIRM (Ribosomal silencing factor RsfS n=3 Tax=Desulforamulus TaxID=2916693 RepID=K8DYK1_9FIRM)

HSP 1 Score: 115 bits (289), Expect = 4.740e-31
Identity = 54/112 (48.21%), Postives = 75/112 (66.96%), Query Frame = 1
Query:    7 LESLEMARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSIEERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWRDAPLVHM 342
            L + E+   A + ADDK   DI V D++ +SI+CDYF+I +  +  Q + +A  IEE L+ AG    R +GFREGSWIL+DYG +V+H+F + ER FY+LE LW DA LV +
Sbjct:    3 LSAKEIVNIAHKAADDKKATDITVLDISHISIICDYFLICTGRSSTQVQAVAEHIEETLEEAGVRPLRIEGFREGSWILLDYGDVVIHVFQEMERGFYNLERLWGDARLVEI 114          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig10933.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7X9AFF1_9BURK9.220e-4357.66Ribosomal silencing factor RsfS n=1 Tax=Burkholder... [more]
A0A7Z9UD74_9BACT2.070e-3955.45Ribosomal silencing factor RsfS n=1 Tax=Phycisphae... [more]
F6B8Q1_DESCC3.550e-3347.41Ribosomal silencing factor RsfS n=1 Tax=Desulfotom... [more]
A0A355TP36_9BACT4.640e-3348.62Ribosomal silencing factor RsfS n=1 Tax=bacterium ... [more]
A0A2N2A2H8_9FIRM1.040e-3142.98Ribosomal silencing factor RsfS n=1 Tax=Firmicutes... [more]
A0A2N2DKM5_9FIRM1.040e-3147.22Ribosomal silencing factor RsfS n=1 Tax=Firmicutes... [more]
UPI001F50C2B01.100e-3147.22ribosome silencing factor n=1 Tax=Acidibacillus sp... [more]
UPI001957FF842.360e-3146.73ribosome silencing factor n=1 Tax=Desulforadius to... [more]
A0A357VG05_9FIRM4.340e-3142.11Ribosomal silencing factor RsfS n=3 Tax=Clostridia... [more]
K8DYK1_9FIRM4.740e-3148.21Ribosomal silencing factor RsfS n=3 Tax=Desulforam... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig10933contigP-wetherbeei_contig10933:87..455 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog696369.KI912183_gene59
Preferred namersfS
PFAMsRsfS
Model size369
Max annot lvl186801|Clostridia
KEGG koko:K09710
Hectar predicted targeting categoryother localisation
Exons1
Evalue2.56e-33
EggNOG OGsCOG0799@1|root,COG0799@2|Bacteria,1VA2Z@1239|Firmicutes,24MVA@186801|Clostridia,262EU@186807|Peptococcaceae
DescriptionFunctions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation
Cds size348
COG categoryJ
BRITEko00000,ko03009
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185890.7860444-UTR-P-wetherbeei_contig10933:86..1071683185890.7860444-UTR-P-wetherbeei_contig10933:86..107Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig10933 87..107 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185890.8062382-CDS-P-wetherbeei_contig10933:107..4551683185890.8062382-CDS-P-wetherbeei_contig10933:107..455Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10933 108..455 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig10933.1.1prot_P-wetherbeei_contig10933.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig10933 108..455 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig10933.1.1

>prot_P-wetherbeei_contig10933.1.1 ID=prot_P-wetherbeei_contig10933.1.1|Name=mRNA_P-wetherbeei_contig10933.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=116bp
MARAAAEVADDKLGRDIIVKDLTDLSIVCDYFVITSAPTRVQTKDIARSI
EERLKGAGQASKRTQGFREGSWILMDYGVIVVHIFLQSEREFYDLEGLWR
DAPLVHMDFQAKKAS*
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mRNA from alignment at P-wetherbeei_contig10933:87..455+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig10933.1.1 ID=mRNA_P-wetherbeei_contig10933.1.1|Name=mRNA_P-wetherbeei_contig10933.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=369bp|location=Sequence derived from alignment at P-wetherbeei_contig10933:87..455+ (Phaeothamnion wetherbeei SAG_119_79)
ATTCTTTTGGAAAGTTTAGAGATGGCTCGGGCTGCCGCGGAAGTTGCGGA TGACAAGCTAGGGCGCGATATTATCGTAAAAGATTTGACGGACCTATCGA TCGTCTGTGATTACTTCGTCATCACCAGCGCCCCGACGCGCGTCCAGACC AAGGACATCGCTCGCTCCATCGAGGAGCGCTTAAAGGGAGCCGGGCAGGC GTCCAAACGCACTCAGGGCTTCCGCGAAGGCTCCTGGATTCTGATGGACT ATGGCGTTATCGTCGTGCACATCTTCTTGCAATCCGAGCGTGAATTTTAT GATCTCGAGGGTCTGTGGCGGGACGCCCCCCTGGTTCACATGGATTTCCA GGCCAAGAAGGCCAGCTAA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig10933:87..455+

>mRNA_P-wetherbeei_contig10933.1.1 ID=mRNA_P-wetherbeei_contig10933.1.1|Name=mRNA_P-wetherbeei_contig10933.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=348bp|location=Sequence derived from alignment at P-wetherbeei_contig10933:87..455+ (Phaeothamnion wetherbeei SAG_119_79)
ATGGCTCGGGCTGCCGCGGAAGTTGCGGATGACAAGCTAGGGCGCGATAT
TATCGTAAAAGATTTGACGGACCTATCGATCGTCTGTGATTACTTCGTCA
TCACCAGCGCCCCGACGCGCGTCCAGACCAAGGACATCGCTCGCTCCATC
GAGGAGCGCTTAAAGGGAGCCGGGCAGGCGTCCAAACGCACTCAGGGCTT
CCGCGAAGGCTCCTGGATTCTGATGGACTATGGCGTTATCGTCGTGCACA
TCTTCTTGCAATCCGAGCGTGAATTTTATGATCTCGAGGGTCTGTGGCGG
GACGCCCCCCTGGTTCACATGGATTTCCAGGCCAAGAAGGCCAGCTAA
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