mRNA_P-wetherbeei_contig10854.1.2 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig10854.1.2
Unique NamemRNA_P-wetherbeei_contig10854.1.2
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: A0A7S4JVR7_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4JVR7_9EUKA)

HSP 1 Score: 162 bits (409), Expect = 3.030e-44
Identity = 77/156 (49.36%), Postives = 109/156 (69.87%), Query Frame = 2
Query:  137 MTNFFLGIALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPDYGGGWYARVPVLAFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFEDVLVFILVPVCILV 604
            + +FFLG+ LSGN+GL+A+VPLFL S++S TSD ++IS  + W+ +  FAV MGI MA+E+I D +PG+ H+LH VM V+HPI GAV +IAP     W+ RVP L  G  +AL++ +GR L+R + SG T G CNP VS  E ++ F + P+ I +
Sbjct:    9 VVSFFLGVTLSGNTGLKATVPLFLLSVLSQTSDCVHISEGLDWIGSVWFAVIMGILMAIEVIADMVPGVAHILHTVMIVVHPIFGAVVAIAPLIDSSWWVRVPTLVLGGVIALVLHIGRMLVRFLVSGATAGFCNPCVSLLESIIAFTVCPLAIFI 164          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: A0A7S0BCY8_9DINO (Hypothetical protein (Fragment) n=1 Tax=Pyrodinium bahamense TaxID=73915 RepID=A0A7S0BCY8_9DINO)

HSP 1 Score: 105 bits (262), Expect = 7.860e-24
Identity = 59/170 (34.71%), Postives = 99/170 (58.24%), Query Frame = 2
Query:  146 FFLGIALSGNSGLRASVPLFLASIM-SLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPDYGGGWYARVPVLAFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFEDVLVFILVPVCILVAFFSVSRMRVF--LSC 646
            + +  AL G +G+R ++P F  S++ ++    + +S++  WL+       +G+ + VE I D +P +DH+LHVV++ +HP+ GA+A++A D  GGW AR PV      LA  +  G+A LR + SG +CG  NP +S  E + V +L+ V IL     +  + +F  L+C
Sbjct:    8 YVIAAALGGGAGIRMTLPAFFLSLLHTVDPGRVPLSTEGEWLTYAGVTSLLGLLVLVETIADMVPALDHLLHVVLAPLHPVMGALAALAVDLCGGWTARAPVALVAGLLAFGVHSGKATLRAVGSGASCGTLNPCLSGCESIGVVLLMAVSILFPVVGLGALALFACLAC 177          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: UPI0019EA9148 (DUF4126 domain-containing protein n=1 Tax=Chloroflexi bacterium AL-N15 TaxID=2686370 RepID=UPI0019EA9148)

HSP 1 Score: 86.7 bits (213), Expect = 7.620e-17
Identity = 62/173 (35.84%), Postives = 93/173 (53.76%), Query Frame = 2
Query:  158 IALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPDYGGGWYARVPVLAFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFEDVLVFILVPVCIL-----------VAFFSVSRMRVFLS 643
            + LS  +GL A +PL    + +  +D I++SS    L+ P   + +GIF  ++ I DKIP IDH+LH +  VIHP+AGA+A +A +   G          G  LA  +   R+ +R +++  T G  NP VS  ED++ F+LV + IL           +AF  V+RMR FL 
Sbjct:   11 LGLSTAAGLNAYIPLLAIGLAARYTDLISLSSPYDLLTNPIVLIAIGIFAILDFIGDKIPAIDHILHAIGVVIHPVAGALAFMAANSTTGTIDPTLAAICGLILAGGMHGTRSAVRPVATTMTGGIANPVVSFIEDIISFVLVILSILAXXXXFVLVVFIAFLVVTRMRRFLQ 183          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: A0A0M0K5R4_9EUKA (Calpain catalytic domain-containing protein (Fragment) n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0K5R4_9EUKA)

HSP 1 Score: 84.3 bits (207), Expect = 1.480e-14
Identity = 53/153 (34.64%), Postives = 84/153 (54.90%), Query Frame = 2
Query:  146 FFLGIALSGNSGLRASVPLFLASIMSLTS-DSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPDYGGGW---YARVPVLAF-GSALALIIFLGRALLRVMSSGGTCGACNPFVSCFEDVLVFILVP 589
            + L   LS  SG+RA  P  + S+M++ + D I + S M WL  P  A    +   +EI+ + +P +DHVLH +M+ +HP+ G + +IAP  G      + + P+  F G  +ALI  L + LLR+  SG  CG  +P ++  E  +V + VP
Sbjct:   14 YALSTVLSSQSGVRAFTPALILSLMAMVNPDVIELDSSMQWLRHPAAAAVFCVLAVLEIVAEFVPAVDHVLHQIMTFVHPVTGIMIAIAPKLGDETVTGFVQAPMAIFDGGTVALIFHLIKLLLRL--SG--CGCLSPVIAAIETTVVVLAVP 162          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: UPI001C3D7722 (DUF4126 family protein n=1 Tax=Miltoncostaea oceani TaxID=2843216 RepID=UPI001C3D7722)

HSP 1 Score: 77.8 bits (190), Expect = 1.370e-13
Identity = 50/139 (35.97%), Postives = 75/139 (53.96%), Query Frame = 2
Query:  158 IALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPDYGGGWYARVPV---LAFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFED 565
            + L+G +GL A +PL L +++    D + +      LS     V + +    + + DKIPG+DHVLHV  +V+HP+AGAV  +    G G    +P    +  G+A A  +  GRA LR +S+  T G  NPF+S  ED
Sbjct:   14 VGLAGAAGLNAWLPLLLGAVLERL-DVVELGDPFGSLSGNAGIVVLTVIFLADFVGDKIPGVDHVLHVAGTVVHPVAGAVLFVG---GTGVDTSIPAVVAMVLGAATAETLHAGRAALRPVSTTTTAGVGNPFLSLAED 148          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: UPI001B075210 (DUF4126 domain-containing protein n=1 Tax=Streptosporangiaceae bacterium NEAU-GS5 TaxID=2820291 RepID=UPI001B075210)

HSP 1 Score: 77.4 bits (189), Expect = 2.350e-13
Identity = 54/164 (32.93%), Postives = 84/164 (51.22%), Query Frame = 2
Query:  155 GIALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPDYGGG-----WYARVPVL--AFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFEDV------LVFILVPVCILVA 607
            G+ LS  +GL A +PL +  +++  +D I++ +   WL+ P     +G+ +  EI+LDKIP +DHV  V+ + + P+AG V   A           W A  P +  A G   ALI+ L +A  R + +  T G   P VS  ED       LV I +P+ ++VA
Sbjct:    7 GLGLSTAAGLNAYIPLMVVGVLAHFTDKIHLPAGYDWLANPAVLAVVGVLLLAEIVLDKIPVVDHVNDVIQTAVRPLAGGVVFSATQAAAAIDNSTWMADHPGVGWALGIVTALIVHLIKAGARPVVNATTVGTGAPVVSTMEDAGSLSLSLVAIFLPLLVIVA 170          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: UPI00164514E9 (DUF4126 domain-containing protein n=1 Tax=Quadrisphaera sp. RL12-1S TaxID=2763011 RepID=UPI00164514E9)

HSP 1 Score: 73.6 bits (179), Expect = 4.680e-12
Identity = 44/144 (30.56%), Postives = 78/144 (54.17%), Query Frame = 2
Query:  152 LGIALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVA------SIAPDYGGGWYARVPVLAFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFED 565
            +G+ L+  +GL A +PL L   ++ ++D + + +  +WLS P     + + + VE++ DK+PG+DH+  V+ +V+ P AG +A      S A D  G W+     +A G+ LAL++ + ++  R   +  T G   P  S  ED
Sbjct:    5 VGLGLASAAGLNAYLPLLLLGALARSTDLVPLPAAWAWLSDPVALAVLAVLLLVEVVADKVPGVDHLNDVLGAVVRPAAGGIAVGAGTASTAGD--GHWW----TVALGAVLALVVTVAKSAGRAAVNATTVGTGAPVASTLED 142          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: A0A315ZX96_9ACTN (Uncharacterized protein DUF4126 n=1 Tax=Quadrisphaera granulorum TaxID=317664 RepID=A0A315ZX96_9ACTN)

HSP 1 Score: 72.8 bits (177), Expect = 8.230e-12
Identity = 40/135 (29.63%), Postives = 75/135 (55.56%), Query Frame = 2
Query:  152 LGIALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVA----SIAPDYGGGWYARVPVLAFGSALALIIFLGRALLRVMSSGGTCGACNP 544
            +G+ L+  +GL A +PL +  +++  +D + +    SWLS P     + + + VE++ DK+PG+DH+  V+ +V+ P AG +A    + + +  G W+  V     G+ALAL++ +G++  R   +  T G   P
Sbjct:    5 VGLGLASAAGLNAYLPLLVLGLLARFTDLVPLPQAWSWLSDPVCLAVLAVLLLVEVVADKVPGVDHLNDVLGAVVRPAAGGIAVGAGTASTNGDGEWWTVV----LGAALALVVTVGKSAARAAVNATTIGTGAP 135          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: UPI001C3CFCA9 (DUF4126 family protein n=1 Tax=Miltoncostaea marina TaxID=2843215 RepID=UPI001C3CFCA9)

HSP 1 Score: 72.8 bits (177), Expect = 9.780e-12
Identity = 47/142 (33.10%), Postives = 73/142 (51.41%), Query Frame = 2
Query:  149 FLGIALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPDYGGGWYARVPVLA---FGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFED 565
            F  + LSG +GL A +PL   +++      + +      LS+      +   +A +++ DK+PG+DH+LHV  +VIHP+AGA+  +    G G    +P       G+A A  +  GRA LR  S+  T G  NP +S  ED
Sbjct:   11 FAAVGLSGAAGLNAYLPLLAGALLERLGV-VELGEPFGALSSDAGIAVLAALLAADVVADKVPGVDHLLHVAGAVIHPVAGALLFVG---GTGPETGIPAAVAAILGAATAETVHGGRAALRPASTATTAGVGNPVLSLAED 148          
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Match: UPI001942426D (DUF4126 domain-containing protein n=3 Tax=Actinoplanes TaxID=1865 RepID=UPI001942426D)

HSP 1 Score: 72.8 bits (177), Expect = 1.150e-11
Identity = 54/173 (31.21%), Postives = 92/173 (53.18%), Query Frame = 2
Query:  137 MTNFFLGIALSGNSGLRASVPLFLASIMSLTSDSINISSDMSWLSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVA--------SIAPDYGGGWYAR---VPVLAFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFEDV------LVFILVPVCILV 604
            M     G  L+ ++GL A +PL +  +++  SD I++ +  +WLS     V +G+ +AVE++ DK+P +DHV  VV +V+ P AG +A        ++     G +++    VPV A G  +AL +   +A+ R + +  T G   P  S  ED+      ++ IL+PV +LV
Sbjct:    1 MLEVLTGTGLAASAGLNAYIPLLVMGLLARYSDLIDLPNGWTWLSNGWVLVILGVLLAVEVVADKVPVVDHVNDVVQTVVRPTAGGLAFGAGSSAQTVTVSDPGSFFSSHQWVPVAA-GVLIALCVHGVKAVARPVVNVSTAGFGAPVASTVEDIGSVLMSVLAILMPVLVLV 172          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig10854.1.2 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S4JVR7_9EUKA3.030e-4449.36Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]
A0A7S0BCY8_9DINO7.860e-2434.71Hypothetical protein (Fragment) n=1 Tax=Pyrodinium... [more]
UPI0019EA91487.620e-1735.84DUF4126 domain-containing protein n=1 Tax=Chlorofl... [more]
A0A0M0K5R4_9EUKA1.480e-1434.64Calpain catalytic domain-containing protein (Fragm... [more]
UPI001C3D77221.370e-1335.97DUF4126 family protein n=1 Tax=Miltoncostaea ocean... [more]
UPI001B0752102.350e-1332.93DUF4126 domain-containing protein n=1 Tax=Streptos... [more]
UPI00164514E94.680e-1230.56DUF4126 domain-containing protein n=1 Tax=Quadrisp... [more]
A0A315ZX96_9ACTN8.230e-1229.63Uncharacterized protein DUF4126 n=1 Tax=Quadrispha... [more]
UPI001C3CFCA99.780e-1233.10DUF4126 family protein n=1 Tax=Miltoncostaea marin... [more]
UPI001942426D1.150e-1131.21DUF4126 domain-containing protein n=3 Tax=Actinopl... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig10854contigP-wetherbeei_contig10854:957..2263 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog1121123.AUAO01000005_gene1796
PFAMsDUF4126
Model size884
Max annot lvl1224|Proteobacteria
Hectar predicted targeting categoryother localisation
Exons3
Evalue5.43e-13
EggNOG OGs2DM5T@1|root,31U0Y@2|Bacteria,1RJ9U@1224|Proteobacteria
DescriptionDomain of unknown function (DUF4126)
Cds size555
COG categoryS
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185887.3802772-UTR-P-wetherbeei_contig10854:956..11701683185887.3802772-UTR-P-wetherbeei_contig10854:956..1170Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig10854 957..1170 -
1683185887.409315-UTR-P-wetherbeei_contig10854:2148..22631683185887.409315-UTR-P-wetherbeei_contig10854:2148..2263Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig10854 2149..2263 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185887.3882012-CDS-P-wetherbeei_contig10854:1170..13521683185887.3882012-CDS-P-wetherbeei_contig10854:1170..1352Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10854 1171..1352 -
1683185887.3952897-CDS-P-wetherbeei_contig10854:1510..17111683185887.3952897-CDS-P-wetherbeei_contig10854:1510..1711Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10854 1511..1711 -
1683185887.4022453-CDS-P-wetherbeei_contig10854:1976..21481683185887.4022453-CDS-P-wetherbeei_contig10854:1976..2148Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10854 1977..2148 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig10854.1.2prot_P-wetherbeei_contig10854.1.2Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig10854 1171..2148 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig10854.1.2

>prot_P-wetherbeei_contig10854.1.2 ID=prot_P-wetherbeei_contig10854.1.2|Name=mRNA_P-wetherbeei_contig10854.1.2|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=185bp
MGGFSGSMTNFFLGIALSGNSGLRASVPLFLASIMSLTSDSINISSDMSW
LSTPTFAVCMGIFMAVEIILDKIPGIDHVLHVVMSVIHPIAGAVASIAPD
YGGGWYARVPVLAFGSALALIIFLGRALLRVMSSGGTCGACNPFVSCFED
VLVFILVPVCILVAFFSVSRMRVFLSCFYLFGRK*
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mRNA from alignment at P-wetherbeei_contig10854:957..2263-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig10854.1.2 ID=mRNA_P-wetherbeei_contig10854.1.2|Name=mRNA_P-wetherbeei_contig10854.1.2|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1307bp|location=Sequence derived from alignment at P-wetherbeei_contig10854:957..2263- (Phaeothamnion wetherbeei SAG_119_79)
CCTTTCCCCAGTAGACCTAAGAGAAGAGTGCGCGAGATATGGGCAATCGA AAAAGCCGCTGACCTTCTCGACTTCCGCATTGTTCGCCCCACCACAGGTT CCTTGTTCTTTCAAAATGGGCGGCTTCAGCGGTAGCATGACAAACTTTTT TCTGGGAATAGCGCTCTCCGGAAACTCCGGACTTCGCGCCTCGGTTCCAC TTTTTCTCGCCAGCATAATGTCGCTCACAAGCGACAGCATCAATATCTCG AGCGACATGTCGTGGCTGTCCACACCGACGTTCGCCGGTGAGCTTCACAA TTTTAGAATTCTAAATTCCTCCTTTCAAGTGTCACGAGTTTTCTCGCATT GTCAAAGGTCTCCTTTTCTCCGTACGAGATCGCCAGTCAGCTATTCAGCG CCGCCCGCTGTTCCGTTCACTGCACCTCAGTCAGCGCCGCTGTTAAATGC CACTGCCTTAAATGCCACTGCCTTAGATGCCACTGCCGTAATGCGACCCA TGCTGATTTCCCTTCCCCCTGCGTTGCAACGAATGCTGGAAACAACAAAC AGTTTGCATGGGCATCTTCATGGCCGTTGAAATTATCCTCGACAAAATCC CCGGAATCGACCATGTCTTGCACGTAGTCATGTCTGTCATCCACCCGATC GCCGGCGCCGTCGCGTCTATCGCGCCGGACTACGGCGGGGGGTGGTACGC TCGAGTCCCGGTCCTCGCCTTTGGCAGCGCGCTCGCACTCATCATTTTCC TCGGTAAATTTGTTGCTTGTGTACGGGGGACGGTCGACGTTAGTTCTTGT GCAAGGTGACTTAGGCGTGCCGCTTCTGACATTACAGAAGGGTGGCAATG AATGTGTTTTTATCTATCTTAAGCTGGCTGCTGTTGTGCTGAAGATCTTT CCCCTGTGCAGGCCGTGCTCTTCTGCGCGTCATGTCATCGGGCGGCACCT GCGGAGCATGCAACCCCTTTGTATCGTGCTTCGAAGATGTTTTGGTCTTC ATTTTGGTGCCGGTGTGCATCCTTGTCGCCTTCTTTTCGGTGAGTCGGAT GAGGGTGTTTCTTTCTTGCTTTTATCTTTTTGGAAGAAAATGAAATGTTA TGGAGTCGTGAGTTCCCTTGAGAAGATGAGAGGAGCGAGGAGAACATCGG CGGGCTGCCGCCGCCACATGCTTATTTGGGCCGTTGCCCCATTTTCATCA GGCGAGGTGCGTTTTCATACATTTATTGCTGGTTAAGGGACATCCTCGTA TACTTCTTACGGACGCAGATAATCGTCGCGGTATGCCTGGTTATCGCGGC GTGCGGC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig10854:957..2263-

>mRNA_P-wetherbeei_contig10854.1.2 ID=mRNA_P-wetherbeei_contig10854.1.2|Name=mRNA_P-wetherbeei_contig10854.1.2|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=555bp|location=Sequence derived from alignment at P-wetherbeei_contig10854:957..2263- (Phaeothamnion wetherbeei SAG_119_79)
ATGGGCGGCTTCAGCGGTAGCATGACAAACTTTTTTCTGGGAATAGCGCT
CTCCGGAAACTCCGGACTTCGCGCCTCGGTTCCACTTTTTCTCGCCAGCA
TAATGTCGCTCACAAGCGACAGCATCAATATCTCGAGCGACATGTCGTGG
CTGTCCACACCGACGTTCGCCGTTTGCATGGGCATCTTCATGGCCGTTGA
AATTATCCTCGACAAAATCCCCGGAATCGACCATGTCTTGCACGTAGTCA
TGTCTGTCATCCACCCGATCGCCGGCGCCGTCGCGTCTATCGCGCCGGAC
TACGGCGGGGGGTGGTACGCTCGAGTCCCGGTCCTCGCCTTTGGCAGCGC
GCTCGCACTCATCATTTTCCTCGGCCGTGCTCTTCTGCGCGTCATGTCAT
CGGGCGGCACCTGCGGAGCATGCAACCCCTTTGTATCGTGCTTCGAAGAT
GTTTTGGTCTTCATTTTGGTGCCGGTGTGCATCCTTGTCGCCTTCTTTTC
GGTGAGTCGGATGAGGGTGTTTCTTTCTTGCTTTTATCTTTTTGGAAGAA
AATGA
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