mRNA_P-wetherbeei_contig10514.4.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig10514.4.1
Unique NamemRNA_P-wetherbeei_contig10514.4.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A3C1FN35_9PROT (NUDIX hydrolase n=3 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A3C1FN35_9PROT)

HSP 1 Score: 314 bits (804), Expect = 4.570e-107
Identity = 150/192 (78.12%), Postives = 164/192 (85.42%), Query Frame = 1
Query:    1 MSKSPTQHPATIGFDFSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGNFPVAGQGF 576
            MSKS + HPAT GFDFS   P GDD+ERLVCN CGF++YVNPKIVVGAV EW GKILMCRRAI PRKG+WTLPAG+MEE ET+EQGAMREAREEANA +EILSLLAVY+IPRISQVQL+YKARL SP+VSAG ES EVA +DWDEIPW EIAFPS  WAL+HYRQVR    F PF NPEGE+GNFP  GQGF
Sbjct:    1 MSKSESHHPATDGFDFSLSVPDGDDRERLVCNDCGFIHYVNPKIVVGAVCEWQGKILMCRRAIPPRKGYWTLPAGYMEERETSEQGAMREAREEANAQIEILSLLAVYNIPRISQVQLIYKARLTSPDVSAGPESTEVALYDWDEIPWEEIAFPSSHWALHHYRQVRHTDRFVPFGNPEGESGNFPPPGQGF 192          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A2D4VIC7_9HYPH (NUDIX hydrolase n=2 Tax=Parvibaculum TaxID=256616 RepID=A0A2D4VIC7_9HYPH)

HSP 1 Score: 248 bits (632), Expect = 7.970e-81
Identity = 119/178 (66.85%), Postives = 139/178 (78.09%), Query Frame = 1
Query:   46 FSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGNF----PVAG 567
            FS R P GD+  R VC+ CGF++YVNP+IVVG+V E + + LMCRR+IEPR+GFWTLPAGFME+GETTE GA REAREEANA++ I  LLAVY+IPRISQVQLMY+A L  P  SAG ESLEVA F WD+IPW E+AFPSV WAL  YR V++ +   PF NPEGENGNF    P AG
Sbjct:   23 FSRRVPDGDNLARDVCDACGFIDYVNPRIVVGSVVEHEERFLMCRRSIEPRRGFWTLPAGFMEQGETTEDGAKREAREEANAEIVIRDLLAVYNIPRISQVQLMYRATLARPEFSAGEESLEVALFAWDDIPWQELAFPSVYWALTQYRSVKEKSAIVPFGNPEGENGNFFPAAPSAG 200          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A2N3BZH8_9PROT (NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacterium HGW-Alphaproteobacteria-3 TaxID=2013666 RepID=A0A2N3BZH8_9PROT)

HSP 1 Score: 246 bits (627), Expect = 5.420e-80
Identity = 113/181 (62.43%), Postives = 141/181 (77.90%), Query Frame = 1
Query:   13 PTQHPATIGFDFSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGNF 555
            P +  A     FS R P GD+  R +C+ CGF++YVNP+IVVG+V   +GK L+CRR+IEPR+G+WTLPAGFME+GETTE GA REAREEANAD+ +  +LA+Y+IPRI+QVQ+MY+A L +P+ SAG ESLEV  F WD+IPWNE+AFPSV WAL  +R V D  + APF NPEGENGNF
Sbjct:   17 PREAGAADDVAFSRRVPTGDNLPRDICDRCGFIHYVNPRIVVGSVVMHEGKFLLCRRSIEPRRGYWTLPAGFMEQGETTEDGARREAREEANADIALRDVLAIYNIPRIAQVQIMYRASLRTPHFSAGEESLEVGLFGWDDIPWNELAFPSVHWALTQFRSVMDRDVIAPFTNPEGENGNF 197          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A2N3EAK3_9PROT (NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacterium HGW-Alphaproteobacteria-12 TaxID=2013658 RepID=A0A2N3EAK3_9PROT)

HSP 1 Score: 246 bits (627), Expect = 5.790e-80
Identity = 117/179 (65.36%), Postives = 143/179 (79.89%), Query Frame = 1
Query:   25 PATIGFDFSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGN-FP 558
            PA I F  S R P+GD+  R +C+ CGF++YVNP+IVVG+V   DG+ L+CRRAIEPR+GFWTLPAGFME+GETTE+GA REAREEANA+L +  +LA+Y+IPRI+QVQ+MY+A L SP  SAG ESLEVA FDWD+IPW+E+AFPSV WAL  +R V D+   APF NP GE GN FP
Sbjct:   25 PADIAF--SRRVPSGDNLPRDICDRCGFIHYVNPRIVVGSVAIHDGRFLLCRRAIEPRRGFWTLPAGFMEQGETTEEGARREAREEANAELVLRDVLAIYNIPRIAQVQIMYRAELASPVFSAGEESLEVALFDWDDIPWDELAFPSVHWALTQFRSVMDLDAIAPFTNPGGETGNLFP 201          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A2E1EH71_9HYPH (NUDIX hydrolase n=2 Tax=Hyphomicrobiales TaxID=356 RepID=A0A2E1EH71_9HYPH)

HSP 1 Score: 245 bits (625), Expect = 7.820e-80
Identity = 114/172 (66.28%), Postives = 138/172 (80.23%), Query Frame = 1
Query:   46 FSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGN-FP 558
            FS R P+GD   R +C+ CGF++YVNPKIVVG+V   +GK L+CRR+IEPR+GFWTLPAGFME+GETTE+GA REAREEANAD+ +  +LA+Y+IPRI+QVQ+MY+A L SP  SAG ESLEVA F W+EIPW E+AFPSV WAL  +R V    + APF NPEGENGN FP
Sbjct:   18 FSRRVPSGDSLPRDICDHCGFIHYVNPKIVVGSVVTHEGKFLLCRRSIEPRRGFWTLPAGFMEQGETTEEGARREAREEANADIALKDVLAIYNIPRIAQVQIMYRATLNSPEFSAGEESLEVALFGWEEIPWEELAFPSVYWALTQFRSVMGKDVIAPFTNPEGENGNLFP 189          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A418WTC7_9PROT (NUDIX domain-containing protein n=1 Tax=Oleomonas cavernae TaxID=2320859 RepID=A0A418WTC7_9PROT)

HSP 1 Score: 244 bits (624), Expect = 8.490e-80
Identity = 119/174 (68.39%), Postives = 133/174 (76.44%), Query Frame = 1
Query:   43 DFSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGNFPVA 564
            DF    P GDD ERLVC  CGFVNYVNPKIVVG+V    G+ L+ RRAI+PR GFWTLPAGFME GET   GA REAREEANA++ I +LLAVYSIPRISQVQLM++A L  P+ S G ESLEV  F WDEIPW EIAFPSVVWALNH+RQV    +F PF NP GE G+ P+A
Sbjct:   13 DFHLTVPDGDDHERLVCRTCGFVNYVNPKIVVGSVVTHQGRFLLARRAIDPRLGFWTLPAGFMEVGETVADGARREAREEANAEIVIDALLAVYSIPRISQVQLMHRATLARPDFSPGAESLEVRLFAWDEIPWGEIAFPSVVWALNHWRQVEGQAVFVPFTNPPGETGDRPLA 186          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A2N3EBT8_9PROT (NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacterium HGW-Alphaproteobacteria-11 TaxID=2013657 RepID=A0A2N3EBT8_9PROT)

HSP 1 Score: 244 bits (624), Expect = 1.150e-79
Identity = 115/172 (66.86%), Postives = 136/172 (79.07%), Query Frame = 1
Query:   46 FSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGN-FP 558
            FS R P GD+  R +C+ CGF++YVNPK+VVG+V  ++G+ILMCRRAIEPRKGFWTLPAGFME+GET E+GA REAREEANA++ +  LLAVYSIPRI+QVQLMY+A L  P+ SAG ESLEVA F WDEIPW  +AFPSV WAL  YR +   +   PF NPEGE GN FP
Sbjct:   21 FSRRVPTGDNLPRDICDRCGFIHYVNPKVVVGSVVSFEGRILMCRRAIEPRKGFWTLPAGFMEQGETAEEGARREAREEANAEIVLKDLLAVYSIPRIAQVQLMYRAELADPSFSAGEESLEVALFSWDEIPWEALAFPSVHWALTQYRLIEGQSSIVPFSNPEGETGNLFP 192          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A0A2E5KQM4_9PROT (NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A2E5KQM4_9PROT)

HSP 1 Score: 242 bits (618), Expect = 5.680e-79
Identity = 106/162 (65.43%), Postives = 135/162 (83.33%), Query Frame = 1
Query:   67 GDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGN 552
            GDD+ RLVC+ CGF+NYVNP++VVGAV  W+ +ILMCRRAIEPR GFWT+PAG++EE E    GA REA EEA AD+++  +LAVY++PRISQVQ++Y+  LCSP V  G+ESL+VA F+WD+IPW E+AFPSV WALNH+R+VRD++ FAPF NPEG+ GN
Sbjct:   13 GDDRPRLVCDTCGFINYVNPRVVVGAVCTWENQILMCRRAIEPRTGFWTIPAGYLEEKEAVIDGAKREAWEEAYADVDLDCILAVYNLPRISQVQIIYRGSLCSPKVKPGLESLDVALFEWDDIPWGEVAFPSVHWALNHFREVRDLSTFAPFANPEGQTGN 174          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: UPI000B123450 (NUDIX hydrolase n=2 Tax=Fodinicurvata TaxID=1121829 RepID=UPI000B123450)

HSP 1 Score: 243 bits (619), Expect = 6.390e-79
Identity = 119/176 (67.61%), Postives = 138/176 (78.41%), Query Frame = 1
Query:   25 PATIGFD-FSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENG 549
            PAT   D F +R P GDD+ERLVC  CGFV+Y NPKIVVG+V EW G+IL+CRRAI PRKG+WTLPAG++E  E   +GA+REAREEA A+LEI  LLAVYSIPRISQVQL+Y+ARL    V+ G ES EV  FDW EIPW+EIAFPSV WALNH+R+VR+   FAPF NP GE G
Sbjct:   16 PATQPDDNFENRVPDGDDRERLVCRDCGFVHYQNPKIVVGSVAEWQGRILLCRRAIHPRKGYWTLPAGYLELQEEPLEGALREAREEACAELEIDRLLAVYSIPRISQVQLIYRARLAHQKVACGPESAEVGLFDWSEIPWDEIAFPSVHWALNHHREVRNRQDFAPFANPPGETG 191          
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Match: A7HSZ7_PARL1 (NUDIX hydrolase n=2 Tax=Parvibaculum lavamentivorans TaxID=256618 RepID=A7HSZ7_PARL1)

HSP 1 Score: 243 bits (620), Expect = 7.930e-79
Identity = 112/188 (59.57%), Postives = 142/188 (75.53%), Query Frame = 1
Query:   13 PTQHPATIGFDFSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVTEWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLEILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNEIAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGN-FPVAGQG 573
            P +  A     FS R P+GD+ ER +C+ CGF+NYVNP+IVVG+V   DGK L+CRRAIEPR+G+WTLPAGFME+GETT++GA REAREEANA++ +  +LA+Y+IPRI+QVQ+MY+A L  P+ SAG E+LE   F+W +IPW E+AFPSV WAL H+R+       APF NPEGENGN FP    G
Sbjct:   24 PQEQGAEAELAFSRRVPSGDNLERDICDRCGFINYVNPRIVVGSVVTHDGKFLLCRRAIEPRRGYWTLPAGFMEQGETTDEGARREAREEANAEIVLRDVLAIYNIPRIAQVQIMYRAELREPSFSAGEETLEAGLFEWKDIPWEELAFPSVYWALMHHREAMGKDAIAPFTNPEGENGNLFPRRPAG 211          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig10514.4.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3C1FN35_9PROT4.570e-10778.13NUDIX hydrolase n=3 Tax=Alphaproteobacteria bacter... [more]
A0A2D4VIC7_9HYPH7.970e-8166.85NUDIX hydrolase n=2 Tax=Parvibaculum TaxID=256616 ... [more]
A0A2N3BZH8_9PROT5.420e-8062.43NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacter... [more]
A0A2N3EAK3_9PROT5.790e-8065.36NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacter... [more]
A0A2E1EH71_9HYPH7.820e-8066.28NUDIX hydrolase n=2 Tax=Hyphomicrobiales TaxID=356... [more]
A0A418WTC7_9PROT8.490e-8068.39NUDIX domain-containing protein n=1 Tax=Oleomonas ... [more]
A0A2N3EBT8_9PROT1.150e-7966.86NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacter... [more]
A0A2E5KQM4_9PROT5.680e-7965.43NUDIX hydrolase n=1 Tax=Alphaproteobacteria bacter... [more]
UPI000B1234506.390e-7967.61NUDIX hydrolase n=2 Tax=Fodinicurvata TaxID=112182... [more]
A7HSZ7_PARL17.930e-7959.57NUDIX hydrolase n=2 Tax=Parvibaculum lavamentivora... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig10514contigP-wetherbeei_contig10514:1447..2037 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog570952.ATVH01000013_gene3101
PFAMsNUDIX,Nudix_N_2
Model size591
Max annot lvl204441|Rhodospirillales
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.73e-80
EggNOG OGsCOG1051@1|root,COG1051@2|Bacteria,1REBW@1224|Proteobacteria,2U72I@28211|Alphaproteobacteria,2JSGG@204441|Rhodospirillales
DescriptionNudix N-terminal
Cds size591
COG categoryF
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185873.5037208-CDS-P-wetherbeei_contig10514:1446..20371683185873.5037208-CDS-P-wetherbeei_contig10514:1446..2037Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10514 1447..2037 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig10514.4.1prot_P-wetherbeei_contig10514.4.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig10514 1447..2037 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig10514.4.1

>prot_P-wetherbeei_contig10514.4.1 ID=prot_P-wetherbeei_contig10514.4.1|Name=mRNA_P-wetherbeei_contig10514.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=197bp
MSKSPTQHPATIGFDFSHRTPAGDDKERLVCNGCGFVNYVNPKIVVGAVT
EWDGKILMCRRAIEPRKGFWTLPAGFMEEGETTEQGAMREAREEANADLE
ILSLLAVYSIPRISQVQLMYKARLCSPNVSAGVESLEVAFFDWDEIPWNE
IAFPSVVWALNHYRQVRDVTLFAPFVNPEGENGNFPVAGQGFKPLD*
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mRNA from alignment at P-wetherbeei_contig10514:1447..2037+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig10514.4.1 ID=mRNA_P-wetherbeei_contig10514.4.1|Name=mRNA_P-wetherbeei_contig10514.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=591bp|location=Sequence derived from alignment at P-wetherbeei_contig10514:1447..2037+ (Phaeothamnion wetherbeei SAG_119_79)
ATGAGCAAATCTCCCACGCAACATCCCGCCACTATTGGCTTTGATTTTTC ACACAGAACACCTGCAGGCGACGATAAAGAACGTCTGGTGTGCAATGGCT GCGGTTTCGTGAATTATGTAAATCCGAAGATCGTGGTGGGCGCCGTGACG GAATGGGATGGAAAAATTCTCATGTGCCGCCGCGCGATTGAACCGCGCAA AGGTTTCTGGACATTGCCTGCGGGGTTCATGGAAGAAGGCGAAACTACCG AACAGGGCGCGATGCGCGAAGCGCGCGAGGAAGCAAACGCCGATCTGGAA ATTTTATCGCTGCTGGCGGTGTACAGCATTCCACGCATAAGCCAGGTGCA ACTGATGTACAAGGCGCGGCTATGCTCGCCCAATGTAAGCGCGGGCGTGG AAAGCCTGGAAGTTGCTTTTTTCGACTGGGATGAAATCCCGTGGAACGAG ATCGCGTTTCCAAGTGTCGTATGGGCGCTCAATCACTATCGCCAGGTGCG CGATGTGACGCTGTTCGCGCCATTCGTAAATCCGGAAGGCGAAAACGGCA ATTTCCCGGTGGCCGGACAGGGCTTTAAGCCACTGGATTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig10514:1447..2037+

>mRNA_P-wetherbeei_contig10514.4.1 ID=mRNA_P-wetherbeei_contig10514.4.1|Name=mRNA_P-wetherbeei_contig10514.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=591bp|location=Sequence derived from alignment at P-wetherbeei_contig10514:1447..2037+ (Phaeothamnion wetherbeei SAG_119_79)
ATGAGCAAATCTCCCACGCAACATCCCGCCACTATTGGCTTTGATTTTTC
ACACAGAACACCTGCAGGCGACGATAAAGAACGTCTGGTGTGCAATGGCT
GCGGTTTCGTGAATTATGTAAATCCGAAGATCGTGGTGGGCGCCGTGACG
GAATGGGATGGAAAAATTCTCATGTGCCGCCGCGCGATTGAACCGCGCAA
AGGTTTCTGGACATTGCCTGCGGGGTTCATGGAAGAAGGCGAAACTACCG
AACAGGGCGCGATGCGCGAAGCGCGCGAGGAAGCAAACGCCGATCTGGAA
ATTTTATCGCTGCTGGCGGTGTACAGCATTCCACGCATAAGCCAGGTGCA
ACTGATGTACAAGGCGCGGCTATGCTCGCCCAATGTAAGCGCGGGCGTGG
AAAGCCTGGAAGTTGCTTTTTTCGACTGGGATGAAATCCCGTGGAACGAG
ATCGCGTTTCCAAGTGTCGTATGGGCGCTCAATCACTATCGCCAGGTGCG
CGATGTGACGCTGTTCGCGCCATTCGTAAATCCGGAAGGCGAAAACGGCA
ATTTCCCGGTGGCCGGACAGGGCTTTAAGCCACTGGATTGA
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