mRNA_P-wetherbeei_contig1041.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig1041.1.1
Unique NamemRNA_P-wetherbeei_contig1041.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: A0A836CJX4_9STRA (Lecithin:cholesterol acyltransferase-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CJX4_9STRA)

HSP 1 Score: 204 bits (520), Expect = 1.880e-59
Identity = 93/158 (58.86%), Postives = 115/158 (72.78%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASGRPVVILAHSYGSTVVKYFLNWVES 474
            GF +SGLEIW GEEC + YFRQR+WGT+ ML   L D+ CWLRHM +D  TG+DPP  KLR+A GLEAAD+V   YWV    I   NLA+IGYTS EM++ASYDWRL+P + E RD ++TRL   +E   E SG P V+++HS+G  VV +FLNWVES
Sbjct:  117 GFTSSGLEIWDGEECLRHYFRQRVWGTSSMLQALLADKMCWLRHMELDSETGLDPPGRKLRSAVGLEAADYVFRGYWVWGKVIE--NLAEIGYTSNEMYMASYDWRLSPWMAEKRDGFLTRLKHTLETALEVSGTPAVMMSHSFGGQVVYFFLNWVES 272          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: A0A7S0Z0P7_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0Z0P7_9CRYP)

HSP 1 Score: 189 bits (479), Expect = 1.860e-57
Identity = 84/157 (53.50%), Postives = 113/157 (71.97%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASGRPVVILAHSYGSTVVKYFLNWVE 471
            G +T+GLE+WQGEECA++YFRQR+WGT  M+H  L +  CWLRHMA++  TG+DP  IKLR+AQG EAADFV+G YWV    I   NLAD+GY    M +A+YDWRL+ + L+ RDR+ +RL + IE   + SG   V+++HS G  ++ YF+ WVE
Sbjct:   26 GIVTTGLELWQGEECAKKYFRQRMWGTMTMVHNMLLNTRCWLRHMALNATTGLDPEGIKLRSAQGFEAADFVLGGYWVWSKLIE--NLADVGYDPASMHMAAYDWRLSFAKLQERDRFFSRLSKTIEGLVKVSGEKAVVVSHSMGGNLLLYFMQWVE 180          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: L1JG30_GUITC (Uncharacterized protein n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JG30_GUITC)

HSP 1 Score: 196 bits (499), Expect = 2.320e-57
Identity = 88/158 (55.70%), Postives = 114/158 (72.15%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASGRPVVILAHSYGSTVVKYFLNWVES 474
            G +T+GLE+W GE+CA+ YFRQR+WGT  M+   L +  CWLRHMA+D  TG+DPPNIKLR+AQG EAADFV+G YWV    I   NLADIGY  + MF+ASYDWRLA  LLE RD++ TRL   +E+  + +G   +++AHS G  V+ YFL+W  +
Sbjct:   36 GIVTTGLELWSGEDCAKGYFRQRMWGTMTMVQNMLLNTKCWLRHMALDPVTGLDPPNIKLRSAQGFEAADFVVGGYWVWSKLIE--NLADIGYDPSSMFMASYDWRLAYPLLEDRDQFFTRLSSQVEVMVDGNGAKAILVAHSMGGNVLFYFLHWATA 191          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: A0A7S0TMJ8_HEMAN (Hypothetical protein (Fragment) n=2 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S0TMJ8_HEMAN)

HSP 1 Score: 191 bits (485), Expect = 1.770e-56
Identity = 86/157 (54.78%), Postives = 114/157 (72.61%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASGRPVVILAHSYGSTVVKYFLNWVE 471
            G +T+GLE+WQGEECA++YFRQR+WGT  M+H  L +  CWLRHMA++  TG+DP  IKLR+AQG EAADFV+G YWV    I   NLAD+GY    M +A+YDWRL+ + L+ RDR+ +RL + +E   + SG  VVI++HS G  +V YF+ WVE
Sbjct:  184 GIVTTGLELWQGEECAKKYFRQRMWGTMTMVHNMLFNTRCWLRHMALNASTGLDPEGIKLRSAQGFEAADFVLGGYWVWSKLIE--NLADVGYDPASMHMAAYDWRLSFAKLQERDRFFSRLSKTVEGLVKVSGEKVVIVSHSMGGNLVLYFMQWVE 338          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: K3W971_GLOUD (Uncharacterized protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3W971_GLOUD)

HSP 1 Score: 196 bits (498), Expect = 1.770e-56
Identity = 90/159 (56.60%), Postives = 117/159 (73.58%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASG-RPVVILAHSYGSTVVKYFLNWVES 474
            GF ++GLEIW G EC+++YFRQR+WGT RML  F+ +Q CW+ H+ ++  TG+DP  IKLR A GLEAAD+VIG +WV    I   NLADIGY S  MF+A+YDWR+APSLLE RD+Y T+L   IE+   +SG R VV++ HSY + V+ YF+ WVES
Sbjct:  146 GFTSTGLEIWNGSECSKEYFRQRMWGTARMLQQFMMNQKCWIEHVMLNRSTGLDPDGIKLRPANGLEAADYVIGGFWVWGKVIE--NLADIGYDSNNMFMAAYDWRMAPSLLEVRDQYFTKLKFMIEMAKASSGGRKVVLVTHSYATQVIYYFMKWVES 302          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: A0A7S4MHX5_9STRA (Hypothetical protein (Fragment) n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4MHX5_9STRA)

HSP 1 Score: 182 bits (461), Expect = 7.970e-56
Identity = 82/158 (51.90%), Postives = 109/158 (68.99%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASGRPVVILAHSYGSTVVKYFLNWVES 474
            GF+TSGLE+W GEECAQ++FR R+WG+  M  TF  D+ CW RH+++D  TGMDPP ++LR+AQG EAAD+ +  YWV    I+  NLAD+GY  + M + SYDWRLA   LE RD Y+TRL   IE  +E SG   ++ +HS G++VV YF   V +
Sbjct:   15 GFVTSGLELWAGEECAQKHFRTRLWGSMSMAQTFFADRECWRRHLSLDPNTGMDPPRVRLRSAQGFEAADYFMATYWVWDKLIT--NLADVGYDGSNMVMMSYDWRLAFPKLEERDGYLTRLKHTIEAYHETSGEKAIVASHSMGTSVVLYFFARVTT 170          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: B8BS64_THAPS (Phospholipid:diacylglycerol acyltransferase (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BS64_THAPS)

HSP 1 Score: 190 bits (483), Expect = 1.030e-55
Identity = 85/156 (54.49%), Postives = 111/156 (71.15%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASGRPVVILAHSYGSTVVKYFLNWV 468
            GF+TSGLE+W G +C +++FRQR+WG+  M  TFL D+ CW  H+++D +TGMDPPNI+LR+AQG EAAD  +  YWV    I   NLAD+GY  + M + +YDWRL   L+E+RD Y T+L   IE  +E+SG  VVI +HS G TVV YFLNWV
Sbjct:   10 GFVTSGLELWAGRDCFKKHFRQRLWGSVSMARTFLADRECWREHLSLDPKTGMDPPNIRLRSAQGFEAADNFVATYWVWSKLIE--NLADVGYDGSMMTMMAYDWRLGYELMETRDGYFTKLKHCIEAHFESSGEKVVIASHSMGGTVVYYFLNWV 163          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: A0A8K1CAY8_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CAY8_PYTOL)

HSP 1 Score: 193 bits (490), Expect = 2.470e-55
Identity = 88/159 (55.35%), Postives = 115/159 (72.33%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEAS-GRPVVILAHSYGSTVVKYFLNWVES 474
            GF ++GLEIWQG EC++ YFRQR+WGT RM+  F+ +Q CWL HM ++  TG+DP  +KLR A GLEAAD+VIG YWV    I   NLADIGY S  M++A YDWRL PSL+E RD+Y T+L   IE+    + GR VV+++HSY + V+ +F+ WVES
Sbjct:  140 GFTSTGLEIWQGGECSKAYFRQRMWGTARMVQQFVLNQKCWLEHMMLNRTTGLDPDGVKLRPASGLEAADYVIGGYWVWGKIIE--NLADIGYDSNSMYMAPYDWRLTPSLMEVRDKYFTKLKYMIEMAKATNDGRKVVVISHSYATQVIYFFMQWVES 296          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: A0A3M6VFG0_9STRA (Uncharacterized protein n=2 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VFG0_9STRA)

HSP 1 Score: 192 bits (487), Expect = 3.170e-55
Identity = 89/159 (55.97%), Postives = 114/159 (71.70%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEASG-RPVVILAHSYGSTVVKYFLNWVES 474
            GF ++GLEIW G EC++ YFRQR+WGT+RML  F+ +Q CWL HM ++  +GMDP  IKLRAA+GLEAADF+IG YWV    +   NLA+IGY S  +++A+YDWRL P LLE RD Y T+L   IE+   +SG R V+++ HSY   V  YFL WVES
Sbjct:   96 GFTSTGLEIWNGSECSKAYFRQRMWGTSRMLQQFMMNQKCWLEHMMLNRSSGMDPDGIKLRAAKGLEAADFLIGGYWVWGKMVE--NLAEIGYDSNHLYMAAYDWRLMPHLLEKRDGYFTKLKYTIEMARMSSGGRKVMLVTHSYAQQVFLYFLKWVES 252          
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Match: A0A662X631_9STRA (Uncharacterized protein n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662X631_9STRA)

HSP 1 Score: 192 bits (489), Expect = 5.100e-55
Identity = 88/159 (55.35%), Postives = 116/159 (72.96%), Query Frame = 1
Query:    1 GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHRTGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFLASYDWRLAPSLLESRDRYMTRLMRHIELEYEAS-GRPVVILAHSYGSTVVKYFLNWVES 474
            GF ++GLEIW G EC++ YFRQR+WGT+RML  F+ +Q CWL HM ++  TGMDP  IKLR+A+GLEAAD++IG +WV    +   NLA+IGY S  +F+A+YDWRL P LLE RD Y T+L   IE+  E+S GR V+++ HSY + V  +FL WVES
Sbjct:  175 GFTSTGLEIWNGSECSKTYFRQRMWGTSRMLQQFMMNQKCWLEHMMLNRSTGMDPEGIKLRSAKGLEAADYLIGGFWVWGKMVE--NLAEIGYDSNNLFMAAYDWRLMPHLLEKRDGYFTKLKHTIEMARESSDGRKVMLVTHSYATQVFLHFLKWVES 331          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig1041.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CJX4_9STRA1.880e-5958.86Lecithin:cholesterol acyltransferase-domain-contai... [more]
A0A7S0Z0P7_9CRYP1.860e-5753.50Hypothetical protein (Fragment) n=1 Tax=Hemiselmis... [more]
L1JG30_GUITC2.320e-5755.70Uncharacterized protein n=1 Tax=Guillardia theta (... [more]
A0A7S0TMJ8_HEMAN1.770e-5654.78Hypothetical protein (Fragment) n=2 Tax=Hemiselmis... [more]
K3W971_GLOUD1.770e-5656.60Uncharacterized protein n=1 Tax=Globisporangium ul... [more]
A0A7S4MHX5_9STRA7.970e-5651.90Hypothetical protein (Fragment) n=1 Tax=Odontella ... [more]
B8BS64_THAPS1.030e-5554.49Phospholipid:diacylglycerol acyltransferase (Fragm... [more]
A0A8K1CAY8_PYTOL2.470e-5555.35Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A3M6VFG0_9STRA3.170e-5555.97Uncharacterized protein n=2 Tax=Peronospora effusa... [more]
A0A662X631_9STRA5.100e-5555.35Uncharacterized protein n=2 Tax=Nothophytophthora ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig1041contigP-wetherbeei_contig1041:1669..2789 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog42099.EPrPV00000019273
PFAMsLCAT
Model size474
Max annot lvl121069|Pythiales
Hectar predicted targeting categoryother localisation
Exons4
Evalue4.12e-60
EggNOG OGsKOG2369@1|root,KOG2369@2759|Eukaryota,1MGHW@121069|Pythiales
DescriptionSource PGD
Cds size474
COG categoryI
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185868.2710264-CDS-P-wetherbeei_contig1041:1668..17821683185868.2710264-CDS-P-wetherbeei_contig1041:1668..1782Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1041 1669..1782 +
1683185868.2811232-CDS-P-wetherbeei_contig1041:1916..20601683185868.2811232-CDS-P-wetherbeei_contig1041:1916..2060Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1041 1917..2060 +
1683185868.2901206-CDS-P-wetherbeei_contig1041:2458..26041683185868.2901206-CDS-P-wetherbeei_contig1041:2458..2604Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1041 2459..2604 +
1683185868.2983222-CDS-P-wetherbeei_contig1041:2719..27891683185868.2983222-CDS-P-wetherbeei_contig1041:2719..2789Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1041 2720..2789 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig1041.1.1prot_P-wetherbeei_contig1041.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig1041 1669..2789 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig1041.1.1

>prot_P-wetherbeei_contig1041.1.1 ID=prot_P-wetherbeei_contig1041.1.1|Name=mRNA_P-wetherbeei_contig1041.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=158bp
GFITSGLEIWQGEECAQQYFRQRIWGTTRMLHTFLTDQACWLRHMAMDHR
TGMDPPNIKLRAAQGLEAADFVIGAYWVSRSQISKNNLADIGYTSTEMFL
ASYDWRLAPSLLESRDRYMTRLMRHIELEYEASGRPVVILAHSYGSTVVK
YFLNWVES
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mRNA from alignment at P-wetherbeei_contig1041:1669..2789+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig1041.1.1 ID=mRNA_P-wetherbeei_contig1041.1.1|Name=mRNA_P-wetherbeei_contig1041.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1121bp|location=Sequence derived from alignment at P-wetherbeei_contig1041:1669..2789+ (Phaeothamnion wetherbeei SAG_119_79)
GGATTCATCACTTCTGGATTGGAGATTTGGCAGGGCGAGGAGTGCGCGCA ACAATACTTCAGGCAGCGGATCTGGGGTACGACGCGCATGCTGCACACCT TTTTGACCGATCAGGTAAGTTTCCTTACTGGCTAGGTAGCTCTCACTACG AGAACTACTGACTAGCCGACTGGCTTTAGAAGAGCGGCTTGTACTTGATC AATCATCCGCTCGTCGCGTTACGTTCGCATAATCAACCTTTTTCTCAGGC ATGCTGGCTGCGCCACATGGCCATGGATCACCGCACTGGCATGGACCCGC CAAACATCAAGCTGCGGGCGGCGCAAGGCCTCGAAGCAGCGGACTTCGTC ATTGGGGCGTACTGGGTAAGCCGGTCTCAAATCAGCAAGAATGTTTCCTT TCAGTGGAGCAGGTAATGGCCCGTTTGCTTACTTTGTGGTGATTGTGATT GTGCGTGTTGTTGCTTTCTCCTCGTGCTGTTGACGTTCCCCCCGGCCGCA ATAGCATACTCACTTGTTTGTTTCCCTTTCTCCGCGGTTGTTGCTGCTTT CCCTCCTTTTTCCAAAAGGTTTGGGCTCAGGTGAGAGATGATGCTGGAAT CTGCAGCTCGGGAACAATGGATTGCAACGGATCCGCTGATTCCTGCGAGC GTTTAGGTAACATGTCCAGCCGCCGTTGACGCACCAAATCACGAGCATTT TCTCAATCCAACTGTTCAGGCGCAGCCTATGCGATTGGAAACCGTTGTGA TGCTTTCTTGCTCCTTCTTCTTCTCGTACAGATCATCGAGAACCTGGCAG ACATCGGCTACACGTCGACAGAGATGTTCCTGGCGTCGTACGACTGGCGG CTGGCGCCGAGCCTTCTGGAGAGCCGCGATCGCTACATGACCCGCCTGAT GCGCCACATCGAGCTCGAATACGAGGCCAGCGGCAGGTTGGTCCGTCGAA ACTTGGTTTATTAGCCTCTCGTCTGTACAGGCTGGCATCCAAGGAATGTC GAACACAGTTATTTCTGAACTTTTTTGTCCGCGTGTGTGTTTGTATCACA GGCCCGTTGTTATATTGGCGCATTCGTACGGCTCCACGGTAGTGAAGTAT TTCCTCAACTGGGTCGAGTCT
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Coding sequence (CDS) from alignment at P-wetherbeei_contig1041:1669..2789+

>mRNA_P-wetherbeei_contig1041.1.1 ID=mRNA_P-wetherbeei_contig1041.1.1|Name=mRNA_P-wetherbeei_contig1041.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=474bp|location=Sequence derived from alignment at P-wetherbeei_contig1041:1669..2789+ (Phaeothamnion wetherbeei SAG_119_79)
GGATTCATCACTTCTGGATTGGAGATTTGGCAGGGCGAGGAGTGCGCGCA
ACAATACTTCAGGCAGCGGATCTGGGGTACGACGCGCATGCTGCACACCT
TTTTGACCGATCAGGCATGCTGGCTGCGCCACATGGCCATGGATCACCGC
ACTGGCATGGACCCGCCAAACATCAAGCTGCGGGCGGCGCAAGGCCTCGA
AGCAGCGGACTTCGTCATTGGGGCGTACTGGGTAAGCCGGTCTCAAATCA
GCAAGAATAACCTGGCAGACATCGGCTACACGTCGACAGAGATGTTCCTG
GCGTCGTACGACTGGCGGCTGGCGCCGAGCCTTCTGGAGAGCCGCGATCG
CTACATGACCCGCCTGATGCGCCACATCGAGCTCGAATACGAGGCCAGCG
GCAGGCCCGTTGTTATATTGGCGCATTCGTACGGCTCCACGGTAGTGAAG
TATTTCCTCAACTGGGTCGAGTCT
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