mRNA_P-wetherbeei_contig1019.4.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig1019.4.1
Unique NamemRNA_P-wetherbeei_contig1019.4.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: D7FW61_ECTSI (Conserved oligomeric Golgi complex subunit 1 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FW61_ECTSI)

HSP 1 Score: 121 bits (304), Expect = 5.530e-27
Identity = 82/188 (43.62%), Postives = 116/188 (61.70%), Query Frame = 1
Query:  115 ERALEVLNVPDAWALLFRRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGASENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALFRQCAAAVAS-----SEGIMRGPEAPFLASQWTYIQQFPARILAGAKRALYR 663
            ERALE ++V  AW+LL    + V  K+ +LQ+MVGSRYHELIESAD+IVSM++T A+V  LL  FP   ++VL +V  +L S G S+  G   R++AA     +       +L+L+AP+A+WAALD ++ L+AA L  +C ++        S G+  G  A FL    TY++QFP RIL+   R L R
Sbjct:    5 ERALEHMSVEKAWSLLDTLSSQVVGKQTELQLMVGSRYHELIESADSIVSMKETGAEVLGLLRKFPQACSNVLQQVPSSL-SPGTSDLSGTDVREDAAARHVME-----VVQLMLHAPAAMWAALDSNKVLEAANLVLRCRSSWTDLSSTFSTGVDSGVAA-FLRGHVTYVEQFPRRILSAGTRLLTR 185          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: A0A836CMC2_9STRA (Conserved oligomeric Golgi complex subunit 1 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CMC2_9STRA)

HSP 1 Score: 106 bits (265), Expect = 7.600e-22
Identity = 83/212 (39.15%), Postives = 115/212 (54.25%), Query Frame = 1
Query:  106 VPLERALEVLNVPDAWALLFRRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGASENEGIVARKEAAKVGTGKSGASAAT-------RLLLNAPSAIWAALDDSRYLDAAALFRQCAA------AVASSEGI-------MRGPEAPFLASQWTYIQQFPARILAGAKRALYRPAVFAA 681
            VP+ERALE     DA ALL +   DV  K+ +L+ MV SRYHELIESAD++V+M++  ++V +LL A P+    +L      L      +      RK    + TGKS +SA +       RL+LNAP+AIW+ALD    L+AA +F +CAA      A A+ + +           +A F+ +QW  + QFPARI   A   L R A  AA
Sbjct:   33 VPIERALEYATAEDARALLVKAAEDVGVKQAELRGMVSSRYHELIESADSVVAMQQLTSEVLSLLDALPTDCRHLLKLGAQVLEPHHLEDQH--PPRKP---LQTGKSDSSAVSANQDSTVRLMLNAPAAIWSALDAGHLLEAATIFMRCAAVMRGGAAAAADQSVPLVQQLRQGSVDAAFVQAQWACMLQFPARISHTALALLSRSAAAAA 239          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: A0A7S3YF54_HETAK (Conserved oligomeric Golgi complex subunit 1 (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3YF54_HETAK)

HSP 1 Score: 63.9 bits (154), Expect = 6.020e-9
Identity = 33/80 (41.25%), Postives = 49/80 (61.25%), Query Frame = 1
Query:  115 ERALEVLNVPDAWALLFRRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGAL 354
            E+ LE L + +   +L     +V  K+ +LQ+MVGSRYHE+I+SAD+IV M++    V  LL AFP     +++ V  AL
Sbjct:   47 EKILESLPIEEVQRMLKDSNRNVQTKQTELQLMVGSRYHEIIDSADSIVEMKEEVQQVVELLIAFPKDYKGLVENVPKAL 126          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: A0A2P6MVW1_9EUKA (Conserved oligomeric Golgi complex subunit 1 n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6MVW1_9EUKA)

HSP 1 Score: 66.6 bits (161), Expect = 1.860e-8
Identity = 46/182 (25.27%), Postives = 87/182 (47.80%), Query Frame = 1
Query:  112 LERALEVLNVPDAWALLFRRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGASENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALFRQCAAAVASSEGIMRGP-------EAPFLASQWTYIQQFPARIL 636
            + +    L+V +  +     R +  E++ +L+++VG+RY +LI+SAD IV MR+ + +    +    +    VL   E +L ++          + E   +  G +  +   ++L++ P  IW  ++++RYL+A+ L+ +      S EG              P L  QW+ I QFP +IL
Sbjct:  151 VNKLFNTLSVEEIRSKEMNMRKETEERRQELRVLVGARYRDLIDSADTIVEMRELSKEAYQKMNRVETGCKKVLGLREDSLLAS-------TTGKMEEKNLRRGNTSTAKKIKILVDTPEQIWREIENNRYLEASQLYLRAKETHQSMEGASDAKTIASIHGSIPLLLHQWSTISQFPPKIL 325          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: A0A433PVZ8_9FUNG (Conserved oligomeric Golgi complex subunit 1 n=1 Tax=Endogone sp. FLAS-F59071 TaxID=2340872 RepID=A0A433PVZ8_9FUNG)

HSP 1 Score: 66.6 bits (161), Expect = 1.890e-8
Identity = 52/173 (30.06%), Postives = 84/173 (48.55%), Query Frame = 1
Query:  136 NVPDAWALLFRRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGASENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALFRQCAAAV----ASSEGIMRGPEA--PFLASQWTYIQQFPARIL 636
            ++PD   +  + RAD+  K+ +L++MVG RY +LI +ADAIVSMRK A  V  +L       N  ++ ++    +     N+ +   +    + T    A+A  +LL++ P  IW AL+   YL AA L+           A  E +M   E   P +  QW  +  F  +I+
Sbjct:   88 SIPDLRLMEKKTRADIESKRQELRLMVGERYRDLISAADAIVSMRKNALSVQDMLDKMQDSCN--VNAIKRRASAMLPGRNKDVATEQRKRYLYT----AAAQIKLLVDVPEQIWHALEQDHYLTAARLYLIAKVVYKNLQAEQEDMMFTVETTFPVVQRQWDAVSHFKTQIV 254          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: U9TL26_RHIID (Conserved oligomeric Golgi complex subunit 1 n=9 Tax=Rhizophagus TaxID=1129544 RepID=U9TL26_RHIID)

HSP 1 Score: 65.9 bits (159), Expect = 3.270e-8
Identity = 55/184 (29.89%), Postives = 91/184 (49.46%), Query Frame = 1
Query:  136 NVPDAWALLFRRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLD--EVEGALHSAGASENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALFRQCAAAVASSEGIMRGPEAPFLAS--------QWTYIQQFPARILAGAKRAL 657
            ++P+  +L  R R+D+ EKK +L++MVG RY +LI++AD+IV+MRK A  +         +L+ + D  +V     S     NE     K+  K     S A    +LL++ P  IW +L+  +YL+A+ L+     A    + +    + PF  S        QW  +  F ++IL  A+  L
Sbjct:   29 SIPELRSLEKRTRSDIEEKKQELRLMVGERYRDLIDAADSIVNMRKCALSI-------QEELHHMQDSCDVHALKRSVRTQVNEDNKGTKDEKKHHLYSSAAQ--IKLLVDVPEQIWRSLESHKYLNASRLYL---IAKLVYKNLQAHDDTPFNVSVTFPVVQRQWDAVSHFKSQILQKARHYL 200          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: A0A6P7T825_OCTVU (Conserved oligomeric Golgi complex subunit 1 n=2 Tax=Octopus vulgaris TaxID=6645 RepID=A0A6P7T825_OCTVU)

HSP 1 Score: 64.3 bits (155), Expect = 1.020e-7
Identity = 52/177 (29.38%), Postives = 86/177 (48.59%), Query Frame = 1
Query:  166 RRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGASENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALF-------------RQCAAAVASSEGIMRGPEAPFLASQWTYIQQFPARILAGAKRAL 657
            + R D+  KK +L+IMVG RY +LIE+AD I  M+K++ +V   +    S++  +  +++    + G S     +A K   +        ++  +LLL+ P  +W+A+D   +L AA  F              QC+A++ S          P L+ QWT I  F A IL G ++ L
Sbjct:   35 KNRLDIERKKEELRIMVGERYRDLIEAADTITDMKKSSQNVMNSI----SKIEELCKQLKQNHLAKGTSYIHRPLADKSRKQTENHFYSVASQIKLLLDMPEKMWSAVDSQEFLLAAQAFLLSRHVHTGLQLEAQCSASILSCF--------PVLSRQWTAISHFKATILQGCRKLL 199          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: UPI001CF4FDA9 (conserved oligomeric Golgi complex subunit 1-like n=3 Tax=Acropora TaxID=6127 RepID=UPI001CF4FDA9)

HSP 1 Score: 64.3 bits (155), Expect = 1.060e-7
Identity = 47/180 (26.11%), Postives = 85/180 (47.22%), Query Frame = 1
Query:  139 VPDAWALLFRRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGASENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALF---RQCAAAVASSEGIMRGPEA----PFLASQWTYIQQFPARILAGAKRAL 657
            V +  A+  + R+D+  KK DL++MVG RY +LI++AD +  M+  +  V     +F   +     E+  +    G   ++ +   +  ++   G    ++  +LL+N P  +W+AL+  ++L AA L+   R   + +    G    P+     P L  QW  I  F   IL G++  L
Sbjct:   47 VVEIRAVEHKTRSDIERKKEDLRLMVGERYRDLIDAADTVTEMKTCSKQV----MSFVQDIQKQCKELHSSHRCHGFGGSQSVANTESTSQTKAGFYTIASQMKLLVNTPEKVWSALERKQHLKAAQLYLFSRHIVSILHIDTGDSSAPKLLSSFPILPKQWAAISHFKDSILQGSRALL 222          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: A0A2C9LUZ1_BIOGL (Conserved oligomeric Golgi complex subunit 1 n=1 Tax=Biomphalaria glabrata TaxID=6526 RepID=A0A2C9LUZ1_BIOGL)

HSP 1 Score: 63.2 bits (152), Expect = 2.500e-7
Identity = 52/171 (30.41%), Postives = 84/171 (49.12%), Query Frame = 1
Query:  166 RRRADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDE--VEGALHSAGASENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALF---RQCAAAVASSEGIMRGPEA--PFLASQWTYIQQFPARILAGAKRAL 657
            + R+D+ +KK DL++MVG RY +LI++AD I  M+ +A +V   ++      +S+  +  V GA       +N+    RKE    GT     +A  +LL++ P  IW+ +D   YL A  L+   R   A++     +        P L  QW  I  F + IL G ++ L
Sbjct:   36 KTRSDIEKKKEDLRVMVGERYRDLIDAADTITDMKNSAENVMKSISRMEDLCSSMRQKHMVRGASFHMSV-KNDSDKNRKELEFYGT-----AAQIKLLMDIPEKIWSNVDSQEYLKATQLYLLARHINASLHLDTQLSADVATWFPVLTRQWAAISHFKSTILQGCRQLL 200          
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Match: K1QV15_CRAGI (Conserved oligomeric Golgi complex subunit 1 n=3 Tax=Crassostrea gigas TaxID=29159 RepID=K1QV15_CRAGI)

HSP 1 Score: 62.0 bits (149), Expect = 5.910e-7
Identity = 52/172 (30.23%), Postives = 82/172 (47.67%), Query Frame = 1
Query:  172 RADVAEKKNDLQIMVGSRYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGASENEGI-VARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAALFR-----QCAAAVASSEGIMRGPEAPFLASQWTYIQQFPARILAGAKRALYRPA 669
            RAD+  KK DL+IMVG RY +LIE+AD I  M+ +A +V + +    +++  +  +++      G S  + +    +   K      G ++  +LLL+ P  IW+ALD+  YL A   F        +  + S +        P L  QW  I  F   IL G +  L +PA
Sbjct:    8 RADIERKKEDLRIMVGERYRDLIEAADTITEMKNSAQNVMSSI----AKMEDMCRQLKQRHMVRGTSFQQRLSTGHQGGKKQEVHFYGIASQIKLLLDMPEKIWSALDEEDYLTATQHFLLSRHIHTSLQLESQQSGNLLSMFPVLTRQWAAISHFRTTILQGCRNVLKQPA 175          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig1019.4.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FW61_ECTSI5.530e-2743.62Conserved oligomeric Golgi complex subunit 1 n=2 T... [more]
A0A836CMC2_9STRA7.600e-2239.15Conserved oligomeric Golgi complex subunit 1 n=1 T... [more]
A0A7S3YF54_HETAK6.020e-941.25Conserved oligomeric Golgi complex subunit 1 (Frag... [more]
A0A2P6MVW1_9EUKA1.860e-825.27Conserved oligomeric Golgi complex subunit 1 n=1 T... [more]
A0A433PVZ8_9FUNG1.890e-830.06Conserved oligomeric Golgi complex subunit 1 n=1 T... [more]
U9TL26_RHIID3.270e-829.89Conserved oligomeric Golgi complex subunit 1 n=9 T... [more]
A0A6P7T825_OCTVU1.020e-729.38Conserved oligomeric Golgi complex subunit 1 n=2 T... [more]
UPI001CF4FDA91.060e-726.11conserved oligomeric Golgi complex subunit 1-like ... [more]
A0A2C9LUZ1_BIOGL2.500e-730.41Conserved oligomeric Golgi complex subunit 1 n=1 T... [more]
K1QV15_CRAGI5.910e-730.23Conserved oligomeric Golgi complex subunit 1 n=3 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig1019contigP-wetherbeei_contig1019:4406..5347 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7FW61
PFAMsVps51
Model size944
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons1
Evalue3.63e-28
EggNOG OGs2E98P@1|root,2SFMQ@2759|Eukaryota
DescriptionVps51/Vps67
Cds size870
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185858.0907316-CDS-P-wetherbeei_contig1019:4405..52751683185858.0907316-CDS-P-wetherbeei_contig1019:4405..5275Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1019 4406..5275 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185858.102359-UTR-P-wetherbeei_contig1019:5275..53471683185858.102359-UTR-P-wetherbeei_contig1019:5275..5347Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig1019 5276..5347 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig1019.4.1prot_P-wetherbeei_contig1019.4.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig1019 4406..5275 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig1019.4.1

>prot_P-wetherbeei_contig1019.4.1 ID=prot_P-wetherbeei_contig1019.4.1|Name=mRNA_P-wetherbeei_contig1019.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=290bp
MAATEEDVQDAVPLERALEVLNVPDAWALLFRRRADVAEKKNDLQIMVGS
RYHELIESADAIVSMRKTAADVAALLTAFPSQLNSVLDEVEGALHSAGAS
ENEGIVARKEAAKVGTGKSGASAATRLLLNAPSAIWAALDDSRYLDAAAL
FRQCAAAVASSEGIMRGPEAPFLASQWTYIQQFPARILAGAKRALYRPAV
FAAGSPSSDNGGGSSVGEAAAALAAVLLVEDVDGGVPAAPGPALFSDALQ
LLLRHRGRYVEAALLGKSRDSPSGAPTEQRRRRRRPRTDG
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mRNA from alignment at P-wetherbeei_contig1019:4406..5347-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig1019.4.1 ID=mRNA_P-wetherbeei_contig1019.4.1|Name=mRNA_P-wetherbeei_contig1019.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=942bp|location=Sequence derived from alignment at P-wetherbeei_contig1019:4406..5347- (Phaeothamnion wetherbeei SAG_119_79)
CACCCATGTGCTAAGGCTTGCGCTTCCTGCATACCGGAGCAGCGTCGTAT CCTATTGTGAAAGTGTGAAAAGATGGCCGCGACCGAGGAGGACGTACAAG ATGCGGTCCCGCTGGAGCGCGCCTTGGAAGTGCTCAATGTACCCGATGCC TGGGCACTCCTGTTTCGTCGCCGCGCAGATGTGGCTGAAAAGAAGAATGA CTTGCAGATTATGGTCGGGTCACGATACCACGAGCTTATCGAGTCTGCGG ATGCCATCGTGTCCATGCGCAAGACTGCCGCTGATGTTGCTGCCCTCCTC ACTGCTTTCCCCAGTCAGCTGAATTCGGTTTTGGACGAGGTGGAGGGAGC CCTTCATAGCGCGGGTGCCTCCGAAAATGAGGGCATCGTCGCGAGAAAGG AGGCTGCAAAAGTAGGGACCGGAAAAAGTGGTGCCAGTGCCGCGACGCGC CTCTTGCTGAACGCGCCTTCTGCCATATGGGCGGCGCTGGACGACAGCAG GTACCTCGACGCCGCCGCGCTCTTTCGGCAATGTGCCGCGGCCGTCGCAT CCAGCGAGGGCATCATGCGCGGCCCCGAGGCGCCCTTCTTAGCGAGCCAA TGGACCTACATTCAGCAGTTTCCGGCTCGCATCCTCGCCGGCGCCAAGCG GGCGCTGTACCGTCCGGCGGTTTTCGCCGCCGGATCTCCCAGTAGCGACA ACGGCGGCGGCAGCAGCGTCGGCGAAGCAGCGGCGGCGCTGGCAGCCGTG CTTCTCGTAGAAGATGTCGACGGCGGCGTCCCGGCTGCGCCAGGCCCGGC GCTGTTCTCGGACGCGCTGCAGCTCCTCTTGCGGCACCGGGGCCGCTACG TGGAAGCCGCGCTGCTCGGCAAAAGCAGGGACTCGCCCAGCGGGGCTCCC ACAGAGCAGCGACGGCGACGACGGCGGCCTCGGACCGACGGC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig1019:4406..5347-

>mRNA_P-wetherbeei_contig1019.4.1 ID=mRNA_P-wetherbeei_contig1019.4.1|Name=mRNA_P-wetherbeei_contig1019.4.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=870bp|location=Sequence derived from alignment at P-wetherbeei_contig1019:4406..5347- (Phaeothamnion wetherbeei SAG_119_79)
ATGGCCGCGACCGAGGAGGACGTACAAGATGCGGTCCCGCTGGAGCGCGC
CTTGGAAGTGCTCAATGTACCCGATGCCTGGGCACTCCTGTTTCGTCGCC
GCGCAGATGTGGCTGAAAAGAAGAATGACTTGCAGATTATGGTCGGGTCA
CGATACCACGAGCTTATCGAGTCTGCGGATGCCATCGTGTCCATGCGCAA
GACTGCCGCTGATGTTGCTGCCCTCCTCACTGCTTTCCCCAGTCAGCTGA
ATTCGGTTTTGGACGAGGTGGAGGGAGCCCTTCATAGCGCGGGTGCCTCC
GAAAATGAGGGCATCGTCGCGAGAAAGGAGGCTGCAAAAGTAGGGACCGG
AAAAAGTGGTGCCAGTGCCGCGACGCGCCTCTTGCTGAACGCGCCTTCTG
CCATATGGGCGGCGCTGGACGACAGCAGGTACCTCGACGCCGCCGCGCTC
TTTCGGCAATGTGCCGCGGCCGTCGCATCCAGCGAGGGCATCATGCGCGG
CCCCGAGGCGCCCTTCTTAGCGAGCCAATGGACCTACATTCAGCAGTTTC
CGGCTCGCATCCTCGCCGGCGCCAAGCGGGCGCTGTACCGTCCGGCGGTT
TTCGCCGCCGGATCTCCCAGTAGCGACAACGGCGGCGGCAGCAGCGTCGG
CGAAGCAGCGGCGGCGCTGGCAGCCGTGCTTCTCGTAGAAGATGTCGACG
GCGGCGTCCCGGCTGCGCCAGGCCCGGCGCTGTTCTCGGACGCGCTGCAG
CTCCTCTTGCGGCACCGGGGCCGCTACGTGGAAGCCGCGCTGCTCGGCAA
AAGCAGGGACTCGCCCAGCGGGGCTCCCACAGAGCAGCGACGGCGACGAC
GGCGGCCTCGGACCGACGGC
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