mRNA_P-canaliculata_contig10030.88.1 (mRNA) Pelvetia canaliculata dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-canaliculata_contig10030.88.1
Unique NamemRNA_P-canaliculata_contig10030.88.1
TypemRNA
OrganismPelvetia canaliculata dioecious (Pelvetia canaliculata dioecious (Channelled wrack))
Homology
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BLAST of mRNA_P-canaliculata_contig10030.88.1 vs. uniprot
Match: D8LD31_ECTSI (Ankyrin repeat-containing protein, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LD31_ECTSI)

HSP 1 Score: 322 bits (825), Expect = 3.290e-104
Identity = 154/199 (77.39%), Postives = 168/199 (84.42%), Query Frame = 1 
Query:    4 SAGDRRGEWPHKFKFLPQLD---RVCALCGKDFVRICTACGKCMECFRVGNKDCVDGADTSVPERPRSPPPPETSEERDWPEYPKTMTRNADGTNVLQKRMERAIYDAIRTGDTHPIVGLIRAGVSPNFKRPAGETCLHAAAYCGEMDLVKDLMGRNSDPTVKDSHGNLPVDLAAMQGKDEVVAYLQPLAPSSTAFTIA 591
            S  D RGEWPH+FKF PQL    +VCA+CGKDFVRICTACGKCMECFR GN+DCV+GA  + P R  SPPP    +ER+WPEYPKTMTRN DGT+VL+KRMERAIY+AIR GDT PIVGLIRAGVSPNFKRPAGETCLHAAAYCG +DLVKDL  R +DP VKDSHGNLPVDLAAMQGKD VV +LQPLAPS T FTIA
Sbjct:   77 SRRDTRGEWPHEFKFRPQLGGSRKVCAVCGKDFVRICTACGKCMECFRAGNRDCVEGAVNNTPPRRLSPPPEALPDEREWPEYPKTMTRNKDGTSVLEKRMERAIYEAIRLGDTEPIVGLIRAGVSPNFKRPAGETCLHAAAYCGALDLVKDLAERKADPAVKDSHGNLPVDLAAMQGKDAVVGFLQPLAPSKTPFTIA 275
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BLAST of mRNA_P-canaliculata_contig10030.88.1 vs. uniprot
Match: A0A6H5KU86_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KU86_9PHAE)

HSP 1 Score: 335 bits (860), Expect = 9.480e-101
Identity = 160/209 (76.56%), Postives = 176/209 (84.21%), Query Frame = 1 
Query:    4 SAGDRRGEWPHKFKFLPQLD---RVCALCGKDFVRICTACGKCMECFRVGNKDCVDGADTSVPERPRSPPPPETSEERDWPEYPKTMTRNADGTNVLQKRMERAIYDAIRTGDTHPIVGLIRAGVSPNFKRPAGETCLHAAAYCGEMDLVKDLMGRNSDPTVKDSHGNLPVDLAAMQGKDEVVAYLQPLAPSSTAFTIAAHKRHRHKQQ 621
            S  D RGEWPH+FKF PQL    +VCA+CGKDFVRICTACGKCMECFR G++DCV+GA  + P R  SPPP    +ER+WPEYPKTMTRN DGT+VL+KRMERAIY+AIR GDT PIVGLIRAGVSPNFKRPAGETCLHAAAYCG +DLVKDL  R +DP VKDSHGNLPVDLAAMQGKDEVV +LQPLAPS T FTIAAH+RHR   Q
Sbjct:  788 SRRDTRGEWPHEFKFRPQLGGSRKVCAVCGKDFVRICTACGKCMECFRAGDRDCVEGAVNNTPPRRLSPPPEALPDEREWPEYPKTMTRNKDGTSVLEKRMERAIYEAIRLGDTEPIVGLIRAGVSPNFKRPAGETCLHAAAYCGALDLVKDLAERKADPAVKDSHGNLPVDLAAMQGKDEVVGFLQPLAPSKTPFTIAAHQRHRRVSQ 996
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BLAST of mRNA_P-canaliculata_contig10030.88.1 vs. uniprot
Match: A0A835YL10_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YL10_9STRA)

HSP 1 Score: 67.4 bits (163), Expect = 4.870e-8
Identity = 60/223 (26.91%), Postives = 86/223 (38.57%), Query Frame = 1 
Query:   34 HKFKFLPQLDRVCALCGKDFVRICTACGKCMECFR---VGNKDCVDGADT---SVPERPRSP------------------------PPPETSEERDWPEYPKTMTRNADGTN-----------------------VLQKRMERAIYDAIRTGDTHP----IVGLIRAGVSPNFKRPAGETCLHAAAYCGEMDLVKDLMGRNSDPTVKDSHGNLPVDLAAMQGK 531
            H+F++       C LC    VRIC+AC +C+ C+R    G   C  G ++   S P    S                         P PE + +  W    K       G N                        LQ R++RAIY A  +         ++ L+R     N++R  GET LHAAAY G +   + ++   +D    DSHGN P+DLA   G+
Sbjct:  700 HRFRYRNVTAPGCTLCELTHVRICSACSRCVCCYREERFGPACCETGDESGRWSPPTDASSXXXXXXXXXXXXXXXXXXXXXILRKPKPEIAGDA-WRPGGKRDAGGGGGGNDXXXXXXXXXXXXXXPAGAPKLHTLQGRVDRAIYAAFTSDGKEERFAALLALLRHSGLHNYRRQTGETALHAAAYHGALRAARAMLDGGADADASDSHGNTPLDLARQHGR 921
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BLAST of mRNA_P-canaliculata_contig10030.88.1 vs. uniprot
Match: A0A672H3I0_SALFA (Uncharacterized protein n=1 Tax=Salarias fasciatus TaxID=181472 RepID=A0A672H3I0_SALFA)

HSP 1 Score: 56.2 bits (134), Expect = 4.640e-6
Identity = 32/91 (35.16%), Postives = 46/91 (50.55%), Query Frame = 1 
Query:  292 RMERAIYDAIRTGDTHPIVGLIRAGVSPNFK-RPAGETCLHAAAYCGEMDLVKDLMGRNSDPTVKDSHGNLPVDLAAMQGKDEVVAYLQPL 561
            R  R     +  G T     L++ G  PN   R  G T LH AA  G +D V+ L+   +DP  +D   ++P DLAA  G ++V ++LQ L
Sbjct:   36 RFGRTALQVMMMGSTPVAQVLLKHGADPNVADRSTGATPLHDAARTGFLDTVRLLVHFGADPRARDHANDVPADLAAQHGHEDVASFLQTL 126
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BLAST of mRNA_P-canaliculata_contig10030.88.1 vs. uniprot
Match: A0A2E6T4H9_9BACT (Uncharacterized protein n=1 Tax=Verrucomicrobiales bacterium TaxID=2026801 RepID=A0A2E6T4H9_9BACT)

HSP 1 Score: 59.7 bits (143), Expect = 8.030e-6
Identity = 40/100 (40.00%), Postives = 55/100 (55.00%), Query Frame = 1 
Query:  271 GTNVLQKRMER--AIYDAIRTGDTHPIVGLIRAGVSPNFKRPAGETCLHAAAYCGEMDLVKDLMGRNSDP--TVK--DSHGNLPVDLAAMQGKDEVVAYL 552
            G NV  K ++   A+ +AI+ G+T     L+  G SPN     G TCLH AAY G++D+ K L+  N+D   TVK     G  P+D AAM GK + +  L
Sbjct:  398 GANVHAKGVKERDALMEAIKIGNTEVAALLLDNGASPNSVDEDGVTCLHIAAYRGQVDICKKLLAGNADKDATVKVGPKKGFKPID-AAMDGKHQEIINL 496
The following BLAST results are available for this feature:
BLAST of mRNA_P-canaliculata_contig10030.88.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D8LD31_ECTSI3.290e-10477.39Ankyrin repeat-containing protein, putative n=1 Ta... [more]
A0A6H5KU86_9PHAE9.480e-10176.56Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A835YL10_9STRA4.870e-826.91Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A672H3I0_SALFA4.640e-635.16Uncharacterized protein n=1 Tax=Salarias fasciatus... [more]
A0A2E6T4H9_9BACT8.030e-640.00Uncharacterized protein n=1 Tax=Verrucomicrobiales... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-canaliculata_contig10030contigP-canaliculata_contig10030:843..3548 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Pelvetia canaliculata dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score62.8
Seed ortholog evalue3.6e-07
Seed eggNOG ortholog471857.Svir_21100
KEGG koko:K06867
EggNOG free text desc.Ankyrin repeat-containing protein
EggNOG OGs2IIAC@201174,4E4DI@85010,COG0666@1,COG0666@2
COG Functional cat.S
Best tax levelPseudonocardiales
Best eggNOG OGNA|NA|NA
BRITEko00000
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionAnkyrin repeat-containing domain
Ec32 orthologEc-11_000240.1
Exons2
Model size1530
Cds size999
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-canaliculata_contig10030.88.1prot_P-canaliculata_contig10030.88.1Pelvetia canaliculata dioeciouspolypeptideP-canaliculata_contig10030 1251..3425 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815576.5022268-UTR-P-canaliculata_contig10030:842..12501622815576.5022268-UTR-P-canaliculata_contig10030:842..1250Pelvetia canaliculata dioeciousUTRP-canaliculata_contig10030 843..1250 -
1693494146.463256-UTR-P-canaliculata_contig10030:842..12501693494146.463256-UTR-P-canaliculata_contig10030:842..1250Pelvetia canaliculata dioeciousUTRP-canaliculata_contig10030 843..1250 -
1622815576.5474644-UTR-P-canaliculata_contig10030:3425..35481622815576.5474644-UTR-P-canaliculata_contig10030:3425..3548Pelvetia canaliculata dioeciousUTRP-canaliculata_contig10030 3426..3548 -
1693494146.5121179-UTR-P-canaliculata_contig10030:3425..35481693494146.5121179-UTR-P-canaliculata_contig10030:3425..3548Pelvetia canaliculata dioeciousUTRP-canaliculata_contig10030 3426..3548 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815576.519489-CDS-P-canaliculata_contig10030:1250..21121622815576.519489-CDS-P-canaliculata_contig10030:1250..2112Pelvetia canaliculata dioeciousCDSP-canaliculata_contig10030 1251..2112 -
1693494146.484542-CDS-P-canaliculata_contig10030:1250..21121693494146.484542-CDS-P-canaliculata_contig10030:1250..2112Pelvetia canaliculata dioeciousCDSP-canaliculata_contig10030 1251..2112 -
1622815576.5335424-CDS-P-canaliculata_contig10030:3288..34251622815576.5335424-CDS-P-canaliculata_contig10030:3288..3425Pelvetia canaliculata dioeciousCDSP-canaliculata_contig10030 3289..3425 -
1693494146.4984515-CDS-P-canaliculata_contig10030:3288..34251693494146.4984515-CDS-P-canaliculata_contig10030:3288..3425Pelvetia canaliculata dioeciousCDSP-canaliculata_contig10030 3289..3425 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-canaliculata_contig10030.88.1

>prot_P-canaliculata_contig10030.88.1 ID=prot_P-canaliculata_contig10030.88.1|Name=mRNA_P-canaliculata_contig10030.88.1|organism=Pelvetia canaliculata dioecious|type=polypeptide|length=333bp
MECFRVGNKDCVDGADTSVPERPRSPPPPETSEERDWPEYPKTMTRNADG
TNVLQKRMERAIYDAIRTGDTHPIVGLIRAGVSPNFKRPAGETCLHAAAY
CGEMDLVKDLMGRNSDPTVKDSHGNLPVDLAAMQGKDEVVAYLQPLAPSS
TAFTIAAHKRHRHKQQLKQQKQQKEYQQQQQQLQQQQQLQQQQEQEQQQQ
QQQQQQFQLRQQQLQLQQQQRQHQQQHHLQQQQQQQYLAPGHPQPHQARQ
GFLPLLPQHLRQQHQRQPQPFQLQQPPPLFAPHNPHGSLPPPSAQFRAQN
SLQQLQPHQQQQPPHQPPQQQQQQQQSHPGRG*
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mRNA from alignment at P-canaliculata_contig10030:843..3548-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-canaliculata_contig10030.88.1 ID=mRNA_P-canaliculata_contig10030.88.1|Name=mRNA_P-canaliculata_contig10030.88.1|organism=Pelvetia canaliculata dioecious|type=mRNA|length=2706bp|location=Sequence derived from alignment at P-canaliculata_contig10030:843..3548- (Pelvetia canaliculata dioecious)
GGGAGTGCAGGCGATAGACGGGGCGAGTGGCCCCACAAGTTCAAATTTTT
GCCGCAGCTCGACCGAGTGTGTGCCTTGTGTGGCAAGGATTTTGTGCGGA
TATGCACCGCTTGCGGGAAATGCATGGAATGCTTCCGTGTGGGAAACAAG
GACTGCGTCGATGGTGCGGACACGAGTGTCCCGGAGCGACCGCGCTCGCC
ACCGCCTCCCGAAACTTCGGAAGAGCGCGATTGGCCGGAGTATCCCAAGA
CGATGACAAGGTTAGCTCCTCCGTAGCCCTACGCACATTATTAATAGGTT
CAAGGTATTTGGCACCGCGAGCTGGTGAAGAATGACGGTGCAAACGTTGC
GATTTTGATCCCCCAGCTCCCGAGATTATTTCAACTTGTTTCATACTGTG
CTTGTGTAGAGCATAATCATTTTCCACCCTCCCTCTTGCGATTGTCTCAA
CTTGCTTCGAAGATACCATTTCTCTCGAGATATCTTCTTAGGAAGTCAAA
GCCTCGAAGTTACAGACTAGGGTTTTTACATTGGAGCCTATCCATGCATA
CACGCCAACATCTCTTAGGAAAAAAAGTGTTACCAGAGGTATACTCCGTT
TTCTTTTTTTCTTCTTAAATGTTGTGTTTTTTTTTTTTCTCACTCTCCCT
TTCGCGCATGTTGTGTGCCCCCCCCCCTCCGCGTCTTTTTCTCGTCTTGC
TTCGCGCCTGGAAATGATCCCCGCGACACAATGAAAGTACGAGTTTACGG
TTACGAAATGCGTCCATCTAGCGCGCTACCGAGATTCGTATATCATTGTA
CAGTATTAATGGTAAAGCCCGTGAAAATGAACGTCGAAGAACTAAACGCG
AGTTGTTGATAGAGCTTTTAGTGCAGCGAAAGCCTTTTTGACCGCCACCC
GTCTCGCGTGATCGCGGTTGTCGAGTAGCCCCTTTCAAATAGCAGATGCA
AAGAGCAAACCTTTGTAAATAGTTGGAACCAACGACCCCCCTCCCCCCTC
ACCTTCAAAGTATTGATGCAATTTTTGTCAATCTTTTTGTCTAGAAAAAA
CATACCGATAACATTGAGGGTTATTGAGTATTTCTTTCATTACGAACTAA
GAAGTGTTCTTGTTTTCCGGTTTCCCCACAAGTGTGTACGTACTATCATT
ACTGTTCGAGCTAATACTGTATTGTACCTAATGGGTAGTGTAGTGTAGTG
TAAGCCATTCAGTTAACATTGCTGTCGAGATTTGTGTGCGAATTGAAATG
AGATCAGAGATCCGACTTAATTACTCGGGTAACAATGTACTAGTGTACAG
CTGAAAATATAACATGTTCGCGGCCTCCGTCTCTCTAGAGACCCAAACCT
GATCTAACTACTCCCCGGCGTCCTCTGTTTCTCCCCCTTCATTTCTCCTG
TACTTTTTTTTTTTTTTTTCCCTTCTCCTTTGCCAGGAATGCGGACGGCA
CGAATGTGCTCCAGAAGCGCATGGAGCGGGCCATTTACGACGCCATTCGC
ACGGGCGACACTCATCCCATTGTCGGCCTCATTAGAGCAGGCGTGTCGCC
GAATTTCAAACGCCCGGCGGGAGAGACCTGTCTCCACGCCGCTGCATACT
GCGGCGAGATGGACCTCGTGAAGGACCTCATGGGGCGGAACTCGGATCCG
ACAGTGAAGGACTCGCACGGAAATCTGCCCGTGGATCTCGCGGCGATGCA
GGGGAAGGATGAAGTCGTGGCGTATTTACAACCTCTCGCTCCATCTTCTA
CAGCTTTCACGATCGCGGCGCACAAGCGACATCGCCATAAGCAGCAGCTC
AAGCAACAGAAGCAGCAGAAGGAGTATCAGCAGCAGCAGCAGCAGTTGCA
GCAGCAGCAGCAGTTGCAGCAGCAGCAAGAACAGGAACAGCAGCAGCAGC
AGCAGCAGCAGCAGCAATTCCAGCTACGACAGCAGCAGCTACAACTGCAG
CAGCAGCAACGGCAACATCAGCAGCAACATCATCTACAGCAGCAGCAGCA
GCAGCAGTACCTGGCACCTGGTCATCCTCAACCGCACCAAGCGCGCCAGG
GGTTTCTGCCGCTCCTTCCTCAGCATCTACGGCAGCAGCATCAACGACAG
CCGCAGCCTTTTCAGCTGCAGCAGCCGCCACCCCTCTTTGCCCCCCACAA
CCCCCACGGTTCTCTCCCCCCCCCTTCTGCTCAGTTTCGTGCGCAAAATT
CTCTGCAGCAGCTTCAGCCGCACCAGCAGCAGCAACCCCCTCATCAGCCG
CCGCAGCAGCAGCAACAACAGCAGCAATCCCACCCTGGGAGGGGGTAGTA
ATAGTAATAGTAGGTTTTCGTATGTCAATCGATTGCGACCATGACAGACA
CAATAATCGTTAAATTCAGCGTGCGATGGCGGGAGGCAAAACGGAATGTA
GCGCGACTGTGTCCATTTTAATACAGGAATCCTATCTTTGTGGCTTTGGG
AGATAAGGTAACGATTGGTGGGAAAGTGGAAAGTAATTCCTTCCTATGTC
TGTCTGTTTGCGGCTGATGTACCTTGAATCATGACGTGCACGTAGAATTG
AAGTAAATAATAAAGCGGTGGGCTCGGTAACATACATCCTTCTGAAAGCC
GTCCGTTTGAAAGAGTATGGTCGATGGACTATTTTCTGGACTCAAGTCGT
TTTTATGTGCGATACGGTACGTTTCTTTGTGTACAAGACTTCGCTGTATT
CTGTTG
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Coding sequence (CDS) from alignment at P-canaliculata_contig10030:843..3548-

>mRNA_P-canaliculata_contig10030.88.1 ID=mRNA_P-canaliculata_contig10030.88.1|Name=mRNA_P-canaliculata_contig10030.88.1|organism=Pelvetia canaliculata dioecious|type=CDS|length=1998bp|location=Sequence derived from alignment at P-canaliculata_contig10030:843..3548- (Pelvetia canaliculata dioecious)
ATGGAATGCTTCCGTGTGGGAAACAAGGACTGCGTCGATGGTGCGGACAC
GAGTGTCCCGGAGCGACCGCGCTCGCCACCGCCTCCCGAAACTTCGGAAG
AGCGCGATTGGCCGGAGTATCCCAAGACGATGACAAGATGGAATGCTTCC
GTGTGGGAAACAAGGACTGCGTCGATGGTGCGGACACGAGTGTCCCGGAG
CGACCGCGCTCGCCACCGCCTCCCGAAACTTCGGAAGAGCGCGATTGGCC
GGAGTATCCCAAGACGATGACAAGGAATGCGGACGGCACGAATGTGCTCC
AGAAGCGCATGGAGCGGGCCATTTACGACGCCATTCGCACGGGCGACACT
CATCCCATTGTCGGCCTCATTAGAGCAGGCGTGTCGCCGAATTTCAAACG
CCCGGCGGGAGAGACCTGTCTCCACGCCGCTGCATACTGCGGCGAGATGG
ACCTCGTGAAGGACCTCATGGGGCGGAACTCGGATCCGACAGTGAAGGAC
TCGCACGGAAATCTGCCCGTGGATCTCGCGGCGATGCAGGGGAAGGATGA
AGTCGTGGCGTATTTACAACCTCTCGCTCCATCTTCTACAGCTTTCACGA
TCGCGGCGCACAAGCGACATCGCCATAAGCAGCAGCTCAAGCAACAGAAG
CAGCAGAAGGAGTATCAGCAGCAGCAGCAGCAGTTGCAGCAGCAGCAGCA
GTTGCAGCAGCAGCAAGAACAGGAACAGCAGCAGCAGCAGCAGCAGCAGC
AGCAATTCCAGCTACGACAGCAGCAGCTACAACTGCAGCAGCAGCAACGG
CAACATCAGCAGCAACATCATCTACAGCAGCAGCAGCAGCAGCAGTACCT
GGCACCTGGTCATCCTCAACCGCACCAAGCGCGCCAGGGGTTTCTGCCGC
TCCTTCCTCAGCATCTACGGCAGCAGCATCAACGACAGCCGCAGCCTTTT
CAGCTGCAGCAGCCGCCACCCCTCTTTGCCCCCCACAACCCCCACGGTTC
TCTCCCCCCCCCTTCTGCTCAGTTTCGTGCGCAAAATTCTCTGCAGCAGC
TTCAGCCGCACCAGCAGCAGCAACCCCCTCATCAGCCGCCGCAGCAGCAG
CAACAACAGCAGCAATCCCACCCTGGGAGGGGGTAGGAATGCGGACGGCA
CGAATGTGCTCCAGAAGCGCATGGAGCGGGCCATTTACGACGCCATTCGC
ACGGGCGACACTCATCCCATTGTCGGCCTCATTAGAGCAGGCGTGTCGCC
GAATTTCAAACGCCCGGCGGGAGAGACCTGTCTCCACGCCGCTGCATACT
GCGGCGAGATGGACCTCGTGAAGGACCTCATGGGGCGGAACTCGGATCCG
ACAGTGAAGGACTCGCACGGAAATCTGCCCGTGGATCTCGCGGCGATGCA
GGGGAAGGATGAAGTCGTGGCGTATTTACAACCTCTCGCTCCATCTTCTA
CAGCTTTCACGATCGCGGCGCACAAGCGACATCGCCATAAGCAGCAGCTC
AAGCAACAGAAGCAGCAGAAGGAGTATCAGCAGCAGCAGCAGCAGTTGCA
GCAGCAGCAGCAGTTGCAGCAGCAGCAAGAACAGGAACAGCAGCAGCAGC
AGCAGCAGCAGCAGCAATTCCAGCTACGACAGCAGCAGCTACAACTGCAG
CAGCAGCAACGGCAACATCAGCAGCAACATCATCTACAGCAGCAGCAGCA
GCAGCAGTACCTGGCACCTGGTCATCCTCAACCGCACCAAGCGCGCCAGG
GGTTTCTGCCGCTCCTTCCTCAGCATCTACGGCAGCAGCATCAACGACAG
CCGCAGCCTTTTCAGCTGCAGCAGCCGCCACCCCTCTTTGCCCCCCACAA
CCCCCACGGTTCTCTCCCCCCCCCTTCTGCTCAGTTTCGTGCGCAAAATT
CTCTGCAGCAGCTTCAGCCGCACCAGCAGCAGCAACCCCCTCATCAGCCG
CCGCAGCAGCAGCAACAACAGCAGCAATCCCACCCTGGGAGGGGGTAG
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