mRNA_M-pyrifera_M_contig114498.3022.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig114498.3022.1
Unique NamemRNA_M-pyrifera_M_contig114498.3022.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A2E7KE14_9PLAN (Uncharacterized protein n=6 Tax=Gimesia TaxID=1649453 RepID=A0A2E7KE14_9PLAN)

HSP 1 Score: 380 bits (975), Expect = 3.740e-122
Identity = 189/190 (99.47%), Postives = 189/190 (99.47%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTA YDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI
Sbjct:  521 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTATYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 710
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A517QDV2_9PLAN (Spermidine synthase n=2 Tax=Gimesia panareensis TaxID=2527978 RepID=A0A517QDV2_9PLAN)

HSP 1 Score: 347 bits (889), Expect = 1.300e-109
Identity = 169/190 (88.95%), Postives = 177/190 (93.16%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SEVEG HGT+TVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIP  LHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDP L+Q+YQ EIATRNSISA DS+RV L+QSPVALAMA+R +    YDLIISNPVQSVVAQSQSEYTADFYRNVSRHLK GG+FCQRFQHIDFGAQPLRVI
Sbjct:  521 SEVEGQHGTFTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPAVLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPSLVQMYQQEIATRNSISAFDSERVRLIQSPVALAMAARTENKTGYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKEGGIFCQRFQHIDFGAQPLRVI 710
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A517VKG2_9PLAN (Spermidine synthase n=1 Tax=Gimesia algae TaxID=2527971 RepID=A0A517VKG2_9PLAN)

HSP 1 Score: 304 bits (778), Expect = 1.420e-93
Identity = 145/190 (76.32%), Postives = 166/190 (87.37%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SE+EG +GT+T+WSY ENQ+QIRRSGLP GVT+ DAGLCPHTTGELMPFVIPV LH+RPADVLFLGLG+GV +NSSLDFPVQH+TCLE D  L++LY +EIA RN+ISA DS+RV LVQ P+ALAMA+R  T   YDLIISNP  SVV QSQ+EYTA+FYRNVSRHL A G+FCQRFQHIDFGAQPLRV+
Sbjct:  521 SELEGQYGTFTIWSYRENQIQIRRSGLPFGVTSVDAGLCPHTTGELMPFVIPVVLHERPADVLFLGLGAGVGVNSSLDFPVQHVTCLEPDRSLVKLYHEEIAPRNAISAFDSERVELVQMPIALAMAARTDTETQYDLIISNPAHSVVTQSQAEYTAEFYRNVSRHLNADGIFCQRFQHIDFGAQPLRVM 710
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A518IJ56_9PLAN (Spermidine synthase n=1 Tax=Gimesia fumaroli TaxID=2527976 RepID=A0A518IJ56_9PLAN)

HSP 1 Score: 302 bits (773), Expect = 6.480e-93
Identity = 144/190 (75.79%), Postives = 167/190 (87.89%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SE++G +GT+T+WSY ENQ+QIRRSGLP GVT+ D GLCPHTTGELMPF+IPV LH+RPADVLFLGLGSGV LN+SLDFPVQH+TCLE D GL++LYQ+EIA RNSISA  S+RV L Q PVALAMA+R++T   YDLIISNP+QSVV QSQ+EYTA+FY NVSRHL   G+FCQRFQHIDFGAQPLRV+
Sbjct:  519 SEIDGEYGTFTLWSYRENQIQIRRSGLPFGVTSVDPGLCPHTTGELMPFLIPVILHERPADVLFLGLGSGVGLNASLDFPVQHVTCLEPDSGLVKLYQEEIAPRNSISAFQSERVQLNQLPVALAMAARSETEKRYDLIISNPIQSVVTQSQAEYTAEFYHNVSRHLNEDGIFCQRFQHIDFGAQPLRVV 708
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A517RMD5_9PLAN (Spermidine synthase n=1 Tax=Gimesia alba TaxID=2527973 RepID=A0A517RMD5_9PLAN)

HSP 1 Score: 302 bits (773), Expect = 6.560e-93
Identity = 144/190 (75.79%), Postives = 168/190 (88.42%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SE++G  GT+T+WSYHENQ+QIRRSGLP GVT+ D GLCPHTTGELMPF+IPV LH+RPADVLFLGLGSGV +N+SLDFPVQ +TCLE D GL++LYQ+EIA RNSISA +S+RV L Q PVALAMA+R++T   YDLIISNPVQSVV QSQ+EYTA+FY+NVSRHL   G+FCQRFQHIDFGAQPLRV+
Sbjct:  520 SEIDGEFGTFTLWSYHENQIQIRRSGLPFGVTSVDPGLCPHTTGELMPFLIPVILHERPADVLFLGLGSGVGVNTSLDFPVQRVTCLESDSGLVKLYQEEIAPRNSISAFESERVQLNQLPVALAMAARSETEKGYDLIISNPVQSVVTQSQAEYTAEFYQNVSRHLNEDGIFCQRFQHIDFGAQPLRVV 709
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A2E2C715_9PLAN (Uncharacterized protein n=4 Tax=Planctomycetaceae TaxID=126 RepID=A0A2E2C715_9PLAN)

HSP 1 Score: 300 bits (769), Expect = 2.740e-92
Identity = 143/190 (75.26%), Postives = 165/190 (86.84%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SEVEG +GT+T+WSY ENQ+QIRRSGLP GVTT DAGLCPHTTGELMPFVIPV LH+RPADVLFLGLG+GV +NSSLDFPVQH+TC+E D  L++LY +EI  RN+ISA DS+RV LVQ+P+ALAMA+RA     YDLIISNPVQSVV QSQ+E+T +FY NVSRHL   G+FCQRFQHIDFGAQPLRV+
Sbjct:  521 SEVEGQYGTFTIWSYRENQIQIRRSGLPFGVTTVDAGLCPHTTGELMPFVIPVILHERPADVLFLGLGAGVGVNSSLDFPVQHVTCMEPDRSLVKLYHEEITPRNAISAFDSERVELVQTPIALAMAARADAEKRYDLIISNPVQSVVTQSQAEFTTEFYHNVSRHLNQDGIFCQRFQHIDFGAQPLRVM 710
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A6CD30_9PLAN (Spermine/spermidine synthase family protein n=7 Tax=Planctomycetaceae TaxID=126 RepID=A6CD30_9PLAN)

HSP 1 Score: 300 bits (768), Expect = 3.030e-92
Identity = 143/190 (75.26%), Postives = 165/190 (86.84%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SEVEG +GT+T+WSY ENQ+QIRRSGLP GVT+ DAGLCPHTTGELMPFVIPV LH+RPADVLFLGLG+GV +NSSLDFPVQH+TCLE D  L++LY +EIA RN+ISA DSDRV L+Q+P+ALAMA+       YDLIISNPVQSVV QSQ+E+T +FYRNVSRHL   G+FCQRFQHIDFGAQPLRV+
Sbjct:  501 SEVEGQYGTFTIWSYRENQIQIRRSGLPFGVTSMDAGLCPHTTGELMPFVIPVILHERPADVLFLGLGAGVGVNSSLDFPVQHVTCLEPDRSLVKLYHEEIAPRNAISAFDSDRVELIQTPIALAMAADTNAEKMYDLIISNPVQSVVTQSQAEFTTEFYRNVSRHLNQDGIFCQRFQHIDFGAQPLRVM 690
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A517VR66_9PLAN (Spermidine synthase n=2 Tax=Gimesia aquarii TaxID=2527964 RepID=A0A517VR66_9PLAN)

HSP 1 Score: 296 bits (759), Expect = 6.570e-91
Identity = 141/190 (74.21%), Postives = 168/190 (88.42%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI 570
            SEVEG +GT+T+WS+ ENQ+QIRRSGLP GVTT D GLCPHTTGELMPF+IPV LH+RP++VLFLGLG+GV +NSSLDFPVQ +TCLE D GL++LY++E+A+RNSISA DS++V L+Q P+ALAMASR ++   YDLIISNPVQSVV QS SEYTA+FY+NVSRHL   G+FCQRFQHIDFGAQPLRVI
Sbjct:  521 SEVEGEYGTFTLWSFRENQIQIRRSGLPFGVTTVDPGLCPHTTGELMPFLIPVILHERPSEVLFLGLGAGVGVNSSLDFPVQKVTCLESDQGLVKLYEEELASRNSISAFDSEQVQLIQLPIALAMASRTESEKQYDLIISNPVQSVVTQSLSEYTAEFYQNVSRHLNEEGIFCQRFQHIDFGAQPLRVI 710
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A350K7R1_9PLAN (Uncharacterized protein (Fragment) n=2 Tax=Planctomycetaceae TaxID=126 RepID=A0A350K7R1_9PLAN)

HSP 1 Score: 201 bits (512), Expect = 3.420e-58
Identity = 93/121 (76.86%), Postives = 108/121 (89.26%), Query Frame = 1 
Query:    1 SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSP 363
            SEVEG +GT+T+WSY ENQ+QIRRSGLP GVT+ DAGLCPHTTGELMPFVIPV LH+RPADVLFLGLG+GV +NSSLDFPVQH+TCLE D  L++LY +EIA RN+ISA DSDRV L+Q+P
Sbjct:  501 SEVEGQYGTFTIWSYRENQIQIRRSGLPFGVTSMDAGLCPHTTGELMPFVIPVILHERPADVLFLGLGAGVGVNSSLDFPVQHVTCLEPDRSLVKLYHEEIAPRNAISAFDSDRVELIQTP 621
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BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Match: A0A518CR10_9PLAN (Spermidine synthase n=1 Tax=Polystyrenella longa TaxID=2528007 RepID=A0A518CR10_9PLAN)

HSP 1 Score: 142 bits (358), Expect = 8.680e-36
Identity = 78/186 (41.94%), Postives = 117/186 (62.90%), Query Frame = 1 
Query:    4 EVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFVIPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDEIATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQSQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPL 561
            +VEG   TYTVWS +  Q  +R  GLP G+ + DA + P  TGE++P V+P+ LH  P  V+ LG+ SGVS+ S L FP+Q + C+E +  +I+L + E A +N  S  +  R  ++  P  LA+ S+A+     D+IIS+P  S++ +S + YT +FY+  +  L   G+FCQRFQH D+G+QPL
Sbjct:  513 QVEGESATYTVWSQNGLQQFVRADGLPQGIASLDASIIPEHTGEILPAVLPLILHRSPHQVMLLGVPSGVSVKSCLQFPIQELKCVETEDAVIELAR-ESAWKNR-SPENDIRFEILDLPARLAL-SQAEP---QDVIISSPPMSMLQRSTNCYTLEFYQAAANALNEEGLFCQRFQHFDYGSQPL 692
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig114498.3022.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E7KE14_9PLAN3.740e-12299.47Uncharacterized protein n=6 Tax=Gimesia TaxID=1649... [more]
A0A517QDV2_9PLAN1.300e-10988.95Spermidine synthase n=2 Tax=Gimesia panareensis Ta... [more]
A0A517VKG2_9PLAN1.420e-9376.32Spermidine synthase n=1 Tax=Gimesia algae TaxID=25... [more]
A0A518IJ56_9PLAN6.480e-9375.79Spermidine synthase n=1 Tax=Gimesia fumaroli TaxID... [more]
A0A517RMD5_9PLAN6.560e-9375.79Spermidine synthase n=1 Tax=Gimesia alba TaxID=252... [more]
A0A2E2C715_9PLAN2.740e-9275.26Uncharacterized protein n=4 Tax=Planctomycetaceae ... [more]
A6CD30_9PLAN3.030e-9275.26Spermine/spermidine synthase family protein n=7 Ta... [more]
A0A517VR66_9PLAN6.570e-9174.21Spermidine synthase n=2 Tax=Gimesia aquarii TaxID=... [more]
A0A350K7R1_9PLAN3.420e-5876.86Uncharacterized protein (Fragment) n=2 Tax=Plancto... [more]
A0A518CR10_9PLAN8.680e-3641.94Spermidine synthase n=1 Tax=Polystyrenella longa T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig114498contigM-pyrifera_M_contig114498:3..572 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score303.9
Seed ortholog evalue5.2e-80
Seed eggNOG ortholog344747.PM8797T_14349
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy- AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine
EggNOG OGs2J20P@203682,COG0421@1,COG0421@2
COG Functional cat.E
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
Exons1
Model size570
Cds size570
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815661.2885578-CDS-M-pyrifera_M_contig114498:2..5721622815661.2885578-CDS-M-pyrifera_M_contig114498:2..572Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig114498 3..572 +
1692277509.8787947-CDS-M-pyrifera_M_contig114498:2..5721692277509.8787947-CDS-M-pyrifera_M_contig114498:2..572Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig114498 3..572 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig114498.3022.1prot_M-pyrifera_M_contig114498.3022.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig114498 3..572 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig114498.3022.1

>prot_M-pyrifera_M_contig114498.3022.1 ID=prot_M-pyrifera_M_contig114498.3022.1|Name=mRNA_M-pyrifera_M_contig114498.3022.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=190bp
SEVEGPHGTYTVWSYHENQLQIRRSGLPVGVTTTDAGLCPHTTGELMPFV
IPVTLHDRPADVLFLGLGSGVSLNSSLDFPVQHITCLEHDPGLIQLYQDE
IATRNSISALDSDRVTLVQSPVALAMASRAKTTAAYDLIISNPVQSVVAQ
SQSEYTADFYRNVSRHLKAGGMFCQRFQHIDFGAQPLRVI
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mRNA from alignment at M-pyrifera_M_contig114498:3..572+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig114498.3022.1 ID=mRNA_M-pyrifera_M_contig114498.3022.1|Name=mRNA_M-pyrifera_M_contig114498.3022.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=570bp|location=Sequence derived from alignment at M-pyrifera_M_contig114498:3..572+ (Macrocystis pyrifera P11B4 male)
TCAGAAGTAGAAGGACCCCACGGAACATATACTGTCTGGTCATATCATGA
AAACCAGCTGCAAATCCGCCGCAGCGGGCTGCCTGTAGGAGTCACCACTA
CCGATGCAGGGCTGTGCCCCCATACAACAGGGGAACTGATGCCTTTTGTG
ATACCGGTAACGTTGCATGACCGCCCCGCCGATGTCTTGTTTCTGGGACT
GGGATCGGGAGTGAGTCTTAACTCCAGCCTGGATTTTCCAGTGCAGCATA
TCACCTGTCTGGAACATGATCCGGGACTGATTCAGTTATATCAGGATGAA
ATTGCTACCCGTAATTCGATCTCGGCACTGGATTCTGACCGCGTCACTCT
GGTGCAGTCCCCTGTCGCTCTGGCCATGGCCTCGCGTGCAAAAACGACTG
CTGCTTATGACCTGATTATCAGTAATCCCGTGCAGTCTGTAGTCGCTCAA
TCTCAGTCAGAGTATACGGCTGATTTTTATCGAAATGTCTCCCGGCATCT
GAAAGCAGGTGGCATGTTCTGCCAGCGCTTCCAGCATATTGATTTTGGCG
CACAACCTCTGCGGGTAATC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig114498:3..572+

>mRNA_M-pyrifera_M_contig114498.3022.1 ID=mRNA_M-pyrifera_M_contig114498.3022.1|Name=mRNA_M-pyrifera_M_contig114498.3022.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1140bp|location=Sequence derived from alignment at M-pyrifera_M_contig114498:3..572+ (Macrocystis pyrifera P11B4 male)
TCAGAAGTAGAAGGACCCCACGGAACATATACTGTCTGGTCATATCATGA
AAACCAGCTGCAAATCCGCCGCAGCGGGCTGCCTGTAGGAGTCACCACTA
CCGATGCAGGGCTGTGCCCCCATACAACAGGGGAACTGATGCCTTTTGTG
ATACCGGTAACGTTGCATGACCGCCCCGCCGATGTCTTGTTTCTGGGACT
GGGATCGGGAGTGAGTCTTAACTCCAGCCTGGATTTTCCAGTGCAGCATA
TCACCTGTCTGGAACATGATCCGGGACTGATTCAGTTATATCAGGATGAA
ATTGCTACCCGTAATTCGATCTCGGCACTGGATTCTGACCGCGTCACTCT
GGTGCAGTCCCCTGTCGCTCTGGCCATGGCCTCGCGTGCAAAAACGACTG
CTGCTTATGACCTGATTATCAGTAATCCCGTGCAGTCTGTAGTCGCTCAA
TCTCAGTCAGAGTATACGGCTGATTTTTATCGAAATGTCTCCCGGCATCT
GAAAGCAGGTGGCATGTTCTGCCAGCGCTTCCAGCATATTGATTTTGGCG
CACAACCTCTGCGGGTAATCTCAGAAGTAGAAGGACCCCACGGAACATAT
ACTGTCTGGTCATATCATGAAAACCAGCTGCAAATCCGCCGCAGCGGGCT
GCCTGTAGGAGTCACCACTACCGATGCAGGGCTGTGCCCCCATACAACAG
GGGAACTGATGCCTTTTGTGATACCGGTAACGTTGCATGACCGCCCCGCC
GATGTCTTGTTTCTGGGACTGGGATCGGGAGTGAGTCTTAACTCCAGCCT
GGATTTTCCAGTGCAGCATATCACCTGTCTGGAACATGATCCGGGACTGA
TTCAGTTATATCAGGATGAAATTGCTACCCGTAATTCGATCTCGGCACTG
GATTCTGACCGCGTCACTCTGGTGCAGTCCCCTGTCGCTCTGGCCATGGC
CTCGCGTGCAAAAACGACTGCTGCTTATGACCTGATTATCAGTAATCCCG
TGCAGTCTGTAGTCGCTCAATCTCAGTCAGAGTATACGGCTGATTTTTAT
CGAAATGTCTCCCGGCATCTGAAAGCAGGTGGCATGTTCTGCCAGCGCTT
CCAGCATATTGATTTTGGCGCACAACCTCTGCGGGTAATC
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