mRNA_M-pyrifera_M_contig9962.22902.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig9962.22902.1
Unique NamemRNA_M-pyrifera_M_contig9962.22902.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: D8LRX5_ECTSI (LigB domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LRX5_ECTSI)

HSP 1 Score: 150 bits (380), Expect = 5.220e-43
Identity = 74/120 (61.67%), Postives = 92/120 (76.67%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMAS-YPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKK-APYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            HLK+G  L+ L  E+DVLIVGSGQATH   S +  Y  G+ AP+E  F+DWLK+TA + +V+PEER+RRL EW++ AP+ R AHPREEHLLP+HV +GCT F PG +IFDDF MG MSLA
Sbjct:  130 HLKMGKTLKKLVDERDVLIVGSGQATHGRGSDHRPYPPGKGAPKEEAFVDWLKETAASPAVTPEERQRRLREWERDAPHGRNAHPREEHLLPLHVAAGCTGFQPGSIIFDDFAMGHMSLA 249          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A2T6E2T7_9GAMM (Dioxygenase n=1 Tax=Saccharospirillum sp. MSK14-1 TaxID=1897632 RepID=A0A2T6E2T7_9GAMM)

HSP 1 Score: 110 bits (275), Expect = 2.360e-27
Identity = 53/118 (44.92%), Postives = 81/118 (68.64%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            HL++G AL +L +EQ V+I+GSG + HNM +Y  +  GEA+P+   F  WL +  T+A +SP++R+  L  W +AP+AR  HPREEHLLP+HV  G  + AP +++FD  ++G  ++A
Sbjct:  147 HLELGRALASL-REQGVMIIGSGFSFHNMRAY-NFQGGEASPQSEAFHQWLVEQLTDADLSPQQRQDALAAWTEAPHARFCHPREEHLLPLHVCVGAANGAPAEIVFDQAMLGQRAIA 262          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A2N2LZT9_9CHLR (Dioxygenase n=1 Tax=Chloroflexi bacterium HGW-Chloroflexi-8 TaxID=2013731 RepID=A0A2N2LZT9_9CHLR)

HSP 1 Score: 106 bits (264), Expect = 5.480e-26
Identity = 54/118 (45.76%), Postives = 78/118 (66.10%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            H+ +G ALR LA E ++L++GSG + HNM ++   A     P    F +WL +T+T+  +S  ER +RL+EW+KAP AR  HPREEHLLP+HV +G  D  P K++FDD ++G   +A
Sbjct:  120 HIALGKALRKLANE-NILVIGSGFSFHNMRAFSWQATDAPDPANDLFQNWLIETSTSP-ISQSEREQRLIEWEKAPSARYCHPREEHLLPLHVCTGMAD-KPAKVVFDDRILGKRGVA 234          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A354Z8M8_9SPIO (Dioxygenase n=1 Tax=Spirochaetaceae bacterium TaxID=1898206 RepID=A0A354Z8M8_9SPIO)

HSP 1 Score: 105 bits (263), Expect = 1.670e-25
Identity = 53/118 (44.92%), Postives = 78/118 (66.10%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            HL +G ALR L  EQ++L++GSG + HN+ ++  + +    PR   F +WL D  T A +S  ER RRL  W++AP+AR  HPREEHLLP+HV +G  +  P ++IFDD ++G  ++A
Sbjct:  158 HLNLGKALRPL-MEQNILVIGSGFSFHNIGAFSWHGKAAPDPRNEAFQNWLIDCCTGA-LSESERERRLSAWEEAPHARYCHPREEHLLPLHVCAGMAN-KPARVIFDDDILGKRAVA 272          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A3D4NFJ2_9CHLR (Dioxygenase n=2 Tax=Bacteria TaxID=2 RepID=A0A3D4NFJ2_9CHLR)

HSP 1 Score: 105 bits (262), Expect = 2.090e-25
Identity = 55/118 (46.61%), Postives = 77/118 (65.25%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            H+ +G ALR L  E ++L++GSG + HNM ++   ++ E  P    F +WL +T T A ++  ER +RL+EW KAP AR  HPREEHLLP+HV  G +D  P KLIFDD ++G   +A
Sbjct:  152 HIGLGKALRELNNE-NILVIGSGFSFHNMRAFSWESKVEPDPGNDAFQNWLIETCT-ADLTQAEREKRLVEWAKAPAARYCHPREEHLLPLHVCQGMSD-KPAKLIFDDLILGKRGVA 266          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: UPI001C58C7A1 (dioxygenase n=1 Tax=Zhongshania sp. CAU 1632 TaxID=2857107 RepID=UPI001C58C7A1)

HSP 1 Score: 104 bits (260), Expect = 3.250e-25
Identity = 56/115 (48.70%), Postives = 79/115 (68.70%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCT--DFAPGKLIFDDFVMG 339
            H+ +G ALR L +EQ+VL++GSG + HN+ ++  Y   +AA R  +F DWL +T T+ ++SP +R RRL+ W  AP AR +HPREEHLLP+HV  G    D A  +LIF+D +MG
Sbjct:  138 HIAMGHALRKL-REQNVLLIGSGLSFHNLQAF-SYV--DAAARAVEFDDWLVETCTDRAISPSDRERRLINWATAPQARFSHPREEHLLPLHVCYGAVARDDAGAELIFNDDMMG 248          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A5K7YQZ9_9DELT (Dioxygenase n=1 Tax=Desulfosarcina alkanivorans TaxID=571177 RepID=A0A5K7YQZ9_9DELT)

HSP 1 Score: 104 bits (260), Expect = 3.600e-25
Identity = 58/118 (49.15%), Postives = 74/118 (62.71%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            HL +G ALR L  E ++LIVGSG + HNM ++   A   +      F +WL D  T  ++S  ER +RL EWKKAP AR  HPREEHLLP+HV  G  D  PG +IFDDF++G   +A
Sbjct:  145 HLALGRALRELLTE-NILIVGSGFSFHNMRAFARDANNVSDRANDDFQNWLIDVCTG-TMSRAEREQRLSEWKKAPSARYCHPREEHLLPLHVCVGMVD-KPGNIIFDDFILGKRGVA 259          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A836CEX8_9STRA (Catalytic LigB subunit of aromatic ring-opening dioxygenase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CEX8_9STRA)

HSP 1 Score: 104 bits (259), Expect = 8.660e-25
Identity = 61/123 (49.59%), Postives = 78/123 (63.41%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASY-PGYARGEAAP---REAQFMDWLKDTATNASVSPEERRRRLLEW-KKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            H+ +G AL  LA  + VL+ GSG ATHN+       A GE  P   R   F+DWLK T  +  VS +ER RRLL W ++AP+AR +HPREEHLLP+HV +G   FA G+L+ D  V G MS+A
Sbjct:  163 HISVGKALSGLAG-RGVLVFGSGMATHNLGMVRSAAAHGEGVPVEPRAGAFIDWLKATLGDG-VSAQERERRLLRWTEEAPHARHSHPREEHLLPLHVAAGAAGFAAGELVLDATVFGGMSMA 283          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A496XGL0_9GAMM (Dioxygenase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A496XGL0_9GAMM)

HSP 1 Score: 103 bits (256), Expect = 1.410e-24
Identity = 55/118 (46.61%), Postives = 74/118 (62.71%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            H+ IG AL  L +E +VLIVGSG + HNM ++   + G    +   F DWL  T TN ++ P ER RRL+EW  AP AR  HPREEHLLP+HV  G ++    + +FDD V+G ++ A
Sbjct:  144 HINIGKALSDLRKE-NVLIVGSGLSFHNMDAFLSKSDGVMDAKNRAFEDWLAHTCTNNNIPPPERERRLIEWADAPAARYCHPREEHLLPLHVCYGLSNSVAAR-VFDDKVLGMLASA 259          
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Match: A0A354A4G4_9BACT (Dioxygenase n=1 Tax=Nitrospiraceae bacterium TaxID=2026770 RepID=A0A354A4G4_9BACT)

HSP 1 Score: 102 bits (254), Expect = 1.660e-24
Identity = 51/118 (43.22%), Postives = 75/118 (63.56%), Query Frame = 1
Query:    1 HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDWLKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDFAPGKLIFDDFVMGAMSLA 354
            H+ +G ALR L   Q++L+VGSG + HNM ++       A P    F DWL ++ T+    P+ R + L+ W+ AP+AR  HPREEHLLP+HV  G +D  P K+IFDD+++G  ++A
Sbjct:  120 HIALGRALREL-MHQNILVVGSGFSFHNMEAFSWQGINAADPSNDSFQDWLIESCTDPIPQPD-REKNLIAWESAPFARYCHPREEHLLPLHVCLGMSD-TPAKVIFDDYILGKRAVA 234          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig9962.22902.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LRX5_ECTSI5.220e-4361.67LigB domain-containing protein n=2 Tax=Ectocarpus ... [more]
A0A2T6E2T7_9GAMM2.360e-2744.92Dioxygenase n=1 Tax=Saccharospirillum sp. MSK14-1 ... [more]
A0A2N2LZT9_9CHLR5.480e-2645.76Dioxygenase n=1 Tax=Chloroflexi bacterium HGW-Chlo... [more]
A0A354Z8M8_9SPIO1.670e-2544.92Dioxygenase n=1 Tax=Spirochaetaceae bacterium TaxI... [more]
A0A3D4NFJ2_9CHLR2.090e-2546.61Dioxygenase n=2 Tax=Bacteria TaxID=2 RepID=A0A3D4N... [more]
UPI001C58C7A13.250e-2548.70dioxygenase n=1 Tax=Zhongshania sp. CAU 1632 TaxID... [more]
A0A5K7YQZ9_9DELT3.600e-2549.15Dioxygenase n=1 Tax=Desulfosarcina alkanivorans Ta... [more]
A0A836CEX8_9STRA8.660e-2549.59Catalytic LigB subunit of aromatic ring-opening di... [more]
A0A496XGL0_9GAMM1.410e-2446.61Dioxygenase n=1 Tax=Gammaproteobacteria bacterium ... [more]
A0A354A4G4_9BACT1.660e-2443.22Dioxygenase n=1 Tax=Nitrospiraceae bacterium TaxID... [more]

Pages

back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig9962contigM-pyrifera_M_contig9962:1879..3225 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score152.9
Seed ortholog evalue9.3e-35
Seed eggNOG ortholog2880.D8LRX5
KEGG rclassRC00387
KEGG koko:K15777
KEGG ReactionR08836
KEGG Pathwayko00965,map00965
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ferrous iron binding
EggNOG OGs2QS66@2759,COG3384@1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons2
Model size354
Cds size354
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816913.2830758-CDS-M-pyrifera_M_contig9962:1878..19501622816913.2830758-CDS-M-pyrifera_M_contig9962:1878..1950Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9962 1879..1950 +
1692278252.041262-CDS-M-pyrifera_M_contig9962:1878..19501692278252.041262-CDS-M-pyrifera_M_contig9962:1878..1950Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9962 1879..1950 +
1622816913.295036-CDS-M-pyrifera_M_contig9962:2943..32251622816913.295036-CDS-M-pyrifera_M_contig9962:2943..3225Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9962 2944..3225 +
1692278252.061715-CDS-M-pyrifera_M_contig9962:2943..32251692278252.061715-CDS-M-pyrifera_M_contig9962:2943..3225Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9962 2944..3225 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig9962.22902.1prot_M-pyrifera_M_contig9962.22902.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig9962 1879..3225 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig9962.22902.1

>prot_M-pyrifera_M_contig9962.22902.1 ID=prot_M-pyrifera_M_contig9962.22902.1|Name=mRNA_M-pyrifera_M_contig9962.22902.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=118bp
HLKIGTALRTLAQEQDVLIVGSGQATHNMASYPGYARGEAAPREAQFMDW
LKDTATNASVSPEERRRRLLEWKKAPYARLAHPREEHLLPMHVVSGCTDF
APGKLIFDDFVMGAMSLA
back to top

mRNA from alignment at M-pyrifera_M_contig9962:1879..3225+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig9962.22902.1 ID=mRNA_M-pyrifera_M_contig9962.22902.1|Name=mRNA_M-pyrifera_M_contig9962.22902.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1347bp|location=Sequence derived from alignment at M-pyrifera_M_contig9962:1879..3225+ (Macrocystis pyrifera P11B4 male)
CACCTCAAAATCGGGACGGCGCTTCGAACGCTAGCCCAAGAACAGGACGT TTTAATCGTCGGCAGCGGCCAGGTGACACAATGTCAGACGTCAAACGAGC GGTCGGTGGTTTTATCGACTTGGCAAGTCGATGGCAAGTCGATGGCAAGT CGATGACCACTATTAATAGGTATCATGGTCTATTTTTCGCTTGGTAAGGT GATGACCAATAAACACGCATGTCGCGGCTGACGGAACTCTCACCCTTTAC TGGACAGCCGTCAAGGATGTCCCCGCACTGAGATTGGTATATCAAGTCAA AATAGCAAAATAGGCCTTGACGGGTCCCATTTTGACCGCCAGCCGACATC TTTGCCATAAATGGCAGAGGCCGCCGGGCAAGGTTGCGTCGCGCGCCTTG AAAAAACACTACTCGCACGAGTAGCGAAACGTGTGTCGTACCTGGTAAGT GTTTTGGGAGCAATTTCTGTGTGTAGATGTTCGTGGCAAGGGTTGGGGAA TGAGGGCGGACAACACCGCTTCCCTTAAAACCTGAAATACACGCTCACGC TTTCGAAGCCAATTCCGCGCGTATCTATCTCCATATATACGTATGATAGC ATCGTGCAAGTTTGTTTTGCGTCTAGTCTTATCGGCCAGAAATCTCATCG GCAGAGCTACAGTACACGGAATTATTCATTATGGTACAAAAGAATTTCCG TGTCTGAAATAATCGTCAGCAAAACTTCGATCAGCAGATTTTTCTTCAGC GAGCAGGTGCCTTGGTATTTCGGCGCTATACATGTATACTCAGTTGGTTG ACTCCCCCGATGTCCCTCCGTGGCCAACTTCACGGCTTGCCCGTAGGAAA CCCGGGCAAAGACCACGAGGGAAAGACCCGATGACCTAATCCGGATTGAC CCAAATGGTCACTGTTTGCGCGACCTACTTTTGTCCCTCGCGATGCTGTT TGTTCGCAATCAGATTATGTTGTCATCAACCCATCGCACTTCCTTTTCCC TCCGCCCCGCCCTTCCCCTACCCCTGGCTCGTTCTCGCCTCCTCCTGCTG CAAAACTATGGGAAGGCGACCCACAACATGGCCTCATACCCGGGATACGC GCGGGGCGAGGCGGCTCCGCGAGAGGCCCAGTTCATGGACTGGCTCAAGG ACACCGCCACGAATGCCTCCGTCTCCCCCGAAGAGCGCCGTCGTCGCTTG CTGGAGTGGAAGAAGGCCCCCTACGCGCGCCTAGCTCACCCCCGCGAGGA GCACCTGTTACCGATGCACGTGGTGTCGGGGTGCACGGACTTCGCGCCGG GCAAACTTATCTTCGACGACTTTGTCATGGGAGCCATGTCTCTCGCT
back to top

Coding sequence (CDS) from alignment at M-pyrifera_M_contig9962:1879..3225+

>mRNA_M-pyrifera_M_contig9962.22902.1 ID=mRNA_M-pyrifera_M_contig9962.22902.1|Name=mRNA_M-pyrifera_M_contig9962.22902.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=708bp|location=Sequence derived from alignment at M-pyrifera_M_contig9962:1879..3225+ (Macrocystis pyrifera P11B4 male)
CACCTCAAAATCGGGACGGCGCTTCGAACGCTAGCCCAAGAACAGGACGT
TTTAATCGTCGGCAGCGGCCAGCACCTCAAAATCGGGACGGCGCTTCGAA
CGCTAGCCCAAGAACAGGACGTTTTAATCGTCGGCAGCGGCCAGGCGACC
CACAACATGGCCTCATACCCGGGATACGCGCGGGGCGAGGCGGCTCCGCG
AGAGGCCCAGTTCATGGACTGGCTCAAGGACACCGCCACGAATGCCTCCG
TCTCCCCCGAAGAGCGCCGTCGTCGCTTGCTGGAGTGGAAGAAGGCCCCC
TACGCGCGCCTAGCTCACCCCCGCGAGGAGCACCTGTTACCGATGCACGT
GGTGTCGGGGTGCACGGACTTCGCGCCGGGCAAACTTATCTTCGACGACT
TTGTCATGGGAGCCATGTCTCTCGCTGCGACCCACAACATGGCCTCATAC
CCGGGATACGCGCGGGGCGAGGCGGCTCCGCGAGAGGCCCAGTTCATGGA
CTGGCTCAAGGACACCGCCACGAATGCCTCCGTCTCCCCCGAAGAGCGCC
GTCGTCGCTTGCTGGAGTGGAAGAAGGCCCCCTACGCGCGCCTAGCTCAC
CCCCGCGAGGAGCACCTGTTACCGATGCACGTGGTGTCGGGGTGCACGGA
CTTCGCGCCGGGCAAACTTATCTTCGACGACTTTGTCATGGGAGCCATGT
CTCTCGCT
back to top