mRNA_M-pyrifera_M_contig99148.22806.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig99148.22806.1
Unique NamemRNA_M-pyrifera_M_contig99148.22806.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: G4YUB5_PHYSP (Uncharacterized protein n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G4YUB5_PHYSP)

HSP 1 Score: 149 bits (377), Expect = 6.280e-40
Identity = 74/156 (47.44%), Postives = 106/156 (67.95%), Query Frame = 1
Query:   31 LGDGSSELEFTEHILEIDDDSKNYHAWSHRQWALKTYGLWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGFANGRISQ-AEGLQEQCRDCVEKYPTSSHALAMLCDILDDKGTAESLAEA 495
            + DGS E    E  ++I  DSKNYHAW+HRQWA+KT+GLW+ EL++V+  L ED+RNNSAWN+R+FV+SNT  L+ AA R+RE+DYAL  +  A +N+SPWNYI G   G  +  A  L+E+ ++ +   P    A  +L D+   +GT +++A A
Sbjct:  296 MRDGSEEKALCEASIDI--DSKNYHAWAHRQWAVKTFGLWDGELEYVDKLLLEDVRNNSAWNYRWFVLSNTSGLATAADRQREVDYALEKVSIAVHNESPWNYIRGLIRGHEAAFAAQLKEKAQNILTATPDCIFAAGLLVDLYAKEGTDDTVASA 449          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A7S0M885_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A7S0M885_9CRYP)

HSP 1 Score: 131 bits (330), Expect = 8.450e-35
Identity = 64/119 (53.78%), Postives = 84/119 (70.59%), Query Frame = 1
Query:    4 HHRRCLVEELGDGSSELEFTEHILEIDD--DSKNYHAWSHRQWALKTYGLWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGFA 354
            HHRRC +E     + E+ F    L  +D  D+KNYHA++HRQW ++TYGLW +EL+F E  LQ+D+ NNSAWNHRFFVVS T D+S AAVR  E+ +AL  +RRA +N S W+Y+ G A
Sbjct:  111 HHRRCCLERGAAPAGEVPFLTAFLTDEDLEDAKNYHAFAHRQWVVQTYGLWAEELEFAEGLLQQDVLNNSAWNHRFFVVSRTTDMS-AAVRTGEVAWALEHLRRAQDNDSAWSYLRGVA 228          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A812E4Q9_SEPPH (FNTA n=1 Tax=Sepia pharaonis TaxID=158019 RepID=A0A812E4Q9_SEPPH)

HSP 1 Score: 125 bits (314), Expect = 4.800e-32
Identity = 59/128 (46.09%), Postives = 82/128 (64.06%), Query Frame = 1
Query:   91 SKNYHAWSHRQWALKTYGLWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYG--FANGRISQAEGLQEQCRDCVEKYPTSSHALAMLCDILDDK 468
            +KNYHAW HRQW LKTY LW+KEL +V+  L  DLRNNSAWNHR+FV+ NT   ++  +   EI+Y   +I + P+N+S WNY+ G    NG + +   + E+C    +K   S + LA L DI +D+
Sbjct:  117 AKNYHAWQHRQWVLKTYSLWDKELNYVDKLLSLDLRNNSAWNHRYFVICNTSKYTDEVIES-EINYTWDYIEQTPHNESAWNYLKGVLMLNG-LREYPQVLEKCLHLYDKNCRSPYLLACLIDIYEDQ 242          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A165K613_EXIGL (Protein prenylyltransferase n=1 Tax=Exidia glandulosa HHB12029 TaxID=1314781 RepID=A0A165K613_EXIGL)

HSP 1 Score: 117 bits (294), Expect = 8.600e-29
Identity = 68/160 (42.50%), Postives = 91/160 (56.88%), Query Frame = 1
Query:    4 HHRRCLVEELGDGSSELEFTEHILEIDDDSKNYHAWSHRQWALKTYG---LWEKELQFVETKLQEDLRNNSAWNHRFFVVSN----TEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGFANGRISQAEGLQEQCRDCVEKYPTSSHALAMLCDILD 462
            HHRR LV +      EL F+ ++L+   DSKNYH W++RQW L  +    LW  EL F+E  L+ED+RNNSAW+HRFFVV +    T D +   V +RE+ +A   I  APNN+S WNY+ G  N   +    L    R  VE Y T +H      D++D
Sbjct:  121 HHRRLLVTKTNKPGPELTFSANVLQT--DSKNYHTWAYRQWVLAHFEQPKLWNGELAFIEAMLEEDVRNNSAWHHRFFVVWDNGVRTGDENREDVIRRELSFAKEKIALAPNNESAWNYLRGVLNRTKTPYATL----RKFVEPY-TVAHPAEPTSDVVD 273          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A5M3MGG0_CONPW (Protein prenylyltransferase n=1 Tax=Coniophora puteana (strain RWD-64-598) TaxID=741705 RepID=A0A5M3MGG0_CONPW)

HSP 1 Score: 115 bits (289), Expect = 4.100e-28
Identity = 61/122 (50.00%), Postives = 75/122 (61.48%), Query Frame = 1
Query:    4 HHRRCLVEELGDGSSELEFTEHILEIDDDSKNYHAWSHRQWALKTYG---LWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTE----DLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYG 348
            HHRR LV    + + EL F    L    D+KNYH WS+RQW L  +    LWE+EL FV   L+EDLRNNSAW+HRFFVV  +     D    AV +REI +A  +I +APNN S WNY+ G
Sbjct:  116 HHRRLLVARTREPARELAFLSRSLRA--DTKNYHTWSYRQWLLAYFNEDTLWERELDFVGDLLEEDLRNNSAWHHRFFVVFQSGVRKGDEDRGAVAQREISFAKQYIAKAPNNASVWNYLRG 235          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A7M5X6S7_9CNID (Uncharacterized protein n=1 Tax=Clytia hemisphaerica TaxID=252671 RepID=A0A7M5X6S7_9CNID)

HSP 1 Score: 109 bits (273), Expect = 4.270e-27
Identity = 59/142 (41.55%), Postives = 83/142 (58.45%), Query Frame = 1
Query:   43 SSELEFTEHILEIDDDSKNYHAWSHRQWALKTYGLWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGFANGRISQAEGLQEQ--CRDCVEKYPTSSHALAMLCDILD 462
            S EL+FT  +L I  D+KNYH W HRQ  L  + LWE E++     L++DLRNNSAWN R++ + NT       + + E+ YA+  I++APNN+S WNY+ G     +S A+GL +    +D  EK         MLCD +D
Sbjct:    2 SFELDFTREMLHI--DAKNYHCWQHRQLVLNHFKLWEGEVELTTILLEKDLRNNSAWNQRYYAIVNTTGFVRETM-ECEVGYAIQMIKKAPNNESAWNYLKGI----LSAADGLHQYPALKDDFEK---------MLCDGMD 127          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A812QPP6_9DINO (FNTA protein n=1 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812QPP6_9DINO)

HSP 1 Score: 113 bits (283), Expect = 5.060e-27
Identity = 63/190 (33.16%), Postives = 102/190 (53.68%), Query Frame = 1
Query:    4 HHRRCLVEELGD------------------GSSELEFTEHILEIDDDSKNYHAWSHRQWALKTYGLWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGF---ANGRI--SQAEGLQEQCRDCVE----KYPTSSHALAMLCDILDDKGTAESLAE 492
            +HRR LV E+                      SEL++    + ++ D KNY+ WSHRQ+ L+ + LW +EL FVE  L+ED+RNNSAWNHR+  V +       ++R RE+++A+  IR+  NN+  WNY+  F    +G++  + A  ++  CR+ VE    +  +   A+  L +I + KG  ++  E
Sbjct:  118 YHRRWLVSEMATRARSRTPGPEAEETIQQIAKSELDYHLECMRVNSDYKNYNGWSHRQFILQQFNLWHEELPFVEQLLEEDIRNNSAWNHRYTTVRHQSWPFTDSIRDRELNFAMQAIRKCANNECAWNYLSAFLGDGDGKVPWTSAPAVEMLCREVVEVATQEGTSCCFAIQALANIHEAKGEIQAALE 307          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: K2RP97_MACPH (Protein prenyltransferase alpha subunit n=1 Tax=Macrophomina phaseolina (strain MS6) TaxID=1126212 RepID=K2RP97_MACPH)

HSP 1 Score: 112 bits (279), Expect = 7.100e-27
Identity = 69/175 (39.43%), Postives = 96/175 (54.86%), Query Frame = 1
Query:    4 HHRRCLVEELGDGSSELEFTEHILEIDDDSKNYHAWSHRQWALKTYGLWEK-ELQFVETKLQEDLRNNSAWNHRFFVV--SNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGFANGRISQAEGLQEQCRDCVEKYP--------TSSHALAMLCDILDDKGTAESLAEA 495
            HHR  +V++LGD + E  F   +L    D          QW +  + LW+K E++ VE  L+ED+RNNSAWNHR+F+V   + E+ S  +V  RE DYA S I+ AP NQSPWNY+ G     I  A+      +D   +Y         +SSHAL +L DI  D+ + E  A+A
Sbjct:  109 HHRTTIVDKLGDATGEQAFIARMLAK--DXXXXXXXXXXQWLVGRFDLWDKGEIEAVEALLREDVRNNSAWNHRWFLVFGGSPENFSQKSVLDREFDYAKSAIKLAPQNQSPWNYLRGI----IRHAKLPLSTLKDFALQYAPVDRPDEISSSHALDLLADIYADESSKEEAAKA 277          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A812GYG0_9DINO (FTA protein n=2 Tax=unclassified Symbiodinium TaxID=279888 RepID=A0A812GYG0_9DINO)

HSP 1 Score: 112 bits (279), Expect = 1.180e-26
Identity = 60/186 (32.26%), Postives = 100/186 (53.76%), Query Frame = 1
Query:    4 HHRRCLVEELGD------------------GSSELEFTEHILEIDDDSKNYHAWSHRQWALKTYGLWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGF---ANGRI--SQAEGLQEQCRDCVE----KYPTSSHALAMLCDILDDKGTAE 480
            +HRR LV E+                      +EL++    + ++ D KNY+ WSHRQ+ L+ + +W +EL FVE  L+ED+RNNSAWNHR+ VV N       ++R RE+ +A+  IR+  +N+  WNY+  F    +G++  + A  L+  CR+ +     + P+   A+  L +I + +G  +
Sbjct:  116 YHRRWLVSEMAARARAHTPGPEAEATIQQIAKTELDYHLECMRVNSDYKNYNGWSHRQYILQHFNMWHEELPFVELLLEEDIRNNSAWNHRYTVVRNQSWPLTDSIRDRELAFAMQAIRKCASNECAWNYLSAFLGEGDGKVPWTSAPALEMLCREVIHAAENQGPSCCFAIQALANIHEARGEVQ 301          
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Match: A0A7S2G5K1_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2G5K1_9STRA)

HSP 1 Score: 107 bits (268), Expect = 1.310e-26
Identity = 50/94 (53.19%), Postives = 64/94 (68.09%), Query Frame = 1
Query:  100 YHAWSHRQWALKTYGLWEKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSWIRRAPNNQSPWNYIYGFANGRISQAEG 381
            YHAWSHRQW LKTYGLW+ EL+ V+  L +D+RNNSAWN R+FV+ NT   +   V  REI YA + I+ A +N+SPWNY+ G    +   A G
Sbjct:    1 YHAWSHRQWVLKTYGLWDGELEVVDELLTQDVRNNSAWNQRWFVIDNTSGRTPEVVA-REIAYAFAKIKVAIDNESPWNYLRGLMRVKGEAAAG 93          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig99148.22806.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
G4YUB5_PHYSP6.280e-4047.44Uncharacterized protein n=1 Tax=Phytophthora sojae... [more]
A0A7S0M885_9CRYP8.450e-3553.78Hypothetical protein (Fragment) n=1 Tax=Cryptomona... [more]
A0A812E4Q9_SEPPH4.800e-3246.09FNTA n=1 Tax=Sepia pharaonis TaxID=158019 RepID=A0... [more]
A0A165K613_EXIGL8.600e-2942.50Protein prenylyltransferase n=1 Tax=Exidia glandul... [more]
A0A5M3MGG0_CONPW4.100e-2850.00Protein prenylyltransferase n=1 Tax=Coniophora put... [more]
A0A7M5X6S7_9CNID4.270e-2741.55Uncharacterized protein n=1 Tax=Clytia hemisphaeri... [more]
A0A812QPP6_9DINO5.060e-2733.16FNTA protein n=1 Tax=Symbiodinium natans TaxID=878... [more]
K2RP97_MACPH7.100e-2739.43Protein prenyltransferase alpha subunit n=1 Tax=Ma... [more]
A0A812GYG0_9DINO1.180e-2632.26FTA protein n=2 Tax=unclassified Symbiodinium TaxI... [more]
A0A7S2G5K1_9STRA1.310e-2653.19Hypothetical protein n=1 Tax=Florenciella parvula ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig99148contigM-pyrifera_M_contig99148:2..496 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score148.7
Seed ortholog evalue2.4e-33
Seed eggNOG ortholog67593.Physo115551
Preferred nameRAM2
KEGG rclassRC00069,RC03004
KEGG koko:K05955
KEGG ReactionR09844
KEGG Pathwayko00900,ko01130,map00900,map01130
Hectar predicted targeting categoryother localisation
GOsGO:0001101,GO:0003674,GO:0003824,GO:0004311,GO:0004659,GO:0004660,GO:0004661,GO:0004662,GO:0004663,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005953,GO:0005965,GO:0006464,GO:0006807,GO:0006950,GO:0007323,GO:0008104,GO:0008150,GO:0008152,GO:0008318,GO:0008360,GO:0009414,GO:0009415,GO:0009628,GO:0009787,GO:0009788,GO:0009966,GO:0009968,GO:0009987,GO:0010035,GO:0010467,GO:0010646,GO:0010648,GO:0016740,GO:0016765,GO:0018342,GO:0018343,GO:0018344,GO:0019538,GO:0022603,GO:0022604,GO:0023051,GO:0023057,GO:0032991,GO:0033036,GO:0034613,GO:0036211,GO:0042221,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0048509,GO:0048519,GO:0048523,GO:0048583,GO:0048585,GO:0050789,GO:0050793,GO:0050794,GO:0050896,GO:0051128,GO:0051179,GO:0051604,GO:0051641,GO:0065007,GO:0065008,GO:0070727,GO:0071704,GO:0072657,GO:0072659,GO:0097354,GO:0140096,GO:1901419,GO:1901420,GO:1901564,GO:1901700,GO:1902494,GO:1905957,GO:1905958,GO:1990234,GO:1990778
EggNOG free text desc.Protein prenyltransferase alpha subunit repeat
EggNOG OGs3Q8DM@4776,COG5536@1,KOG0530@2759
EC2.5.1.58,2.5.1.59
COG Functional cat.O
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01006
Exons1
Model size495
Cds size495
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816908.159456-CDS-M-pyrifera_M_contig99148:1..4961622816908.159456-CDS-M-pyrifera_M_contig99148:1..496Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99148 2..496 -
1692278248.9709563-CDS-M-pyrifera_M_contig99148:1..4961692278248.9709563-CDS-M-pyrifera_M_contig99148:1..496Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99148 2..496 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig99148.22806.1prot_M-pyrifera_M_contig99148.22806.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig99148 2..496 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig99148.22806.1

>prot_M-pyrifera_M_contig99148.22806.1 ID=prot_M-pyrifera_M_contig99148.22806.1|Name=mRNA_M-pyrifera_M_contig99148.22806.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=165bp
RHHRRCLVEELGDGSSELEFTEHILEIDDDSKNYHAWSHRQWALKTYGLW
EKELQFVETKLQEDLRNNSAWNHRFFVVSNTEDLSNAAVRKREIDYALSW
IRRAPNNQSPWNYIYGFANGRISQAEGLQEQCRDCVEKYPTSSHALAMLC
DILDDKGTAESLAEA
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mRNA from alignment at M-pyrifera_M_contig99148:2..496-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig99148.22806.1 ID=mRNA_M-pyrifera_M_contig99148.22806.1|Name=mRNA_M-pyrifera_M_contig99148.22806.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=495bp|location=Sequence derived from alignment at M-pyrifera_M_contig99148:2..496- (Macrocystis pyrifera P11B4 male)
AGGCACCACAGGCGGTGCCTGGTGGAGGAGCTGGGTGATGGCAGCAGCGA GCTAGAGTTCACTGAGCACATCCTCGAGATAGACGACGACTCGAAGAACT ACCATGCTTGGTCCCACCGGCAGTGGGCGCTGAAGACGTACGGCCTCTGG GAGAAGGAGCTGCAATTCGTCGAGACGAAACTGCAGGAGGACCTGAGGAA TAACTCTGCTTGGAACCACCGGTTTTTTGTGGTCTCCAACACAGAGGACC TCTCGAATGCTGCGGTGCGAAAGCGAGAGATCGATTACGCCCTCTCCTGG ATCAGGAGGGCGCCCAACAACCAAAGCCCATGGAATTACATCTACGGCTT TGCAAACGGCCGGATATCACAGGCAGAGGGCCTCCAGGAGCAGTGCCGCG ACTGTGTCGAGAAATACCCCACCTCTTCGCACGCCCTCGCGATGCTCTGC GACATCCTCGACGACAAGGGCACCGCCGAGTCTCTTGCCGAAGCC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig99148:2..496-

>mRNA_M-pyrifera_M_contig99148.22806.1 ID=mRNA_M-pyrifera_M_contig99148.22806.1|Name=mRNA_M-pyrifera_M_contig99148.22806.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=990bp|location=Sequence derived from alignment at M-pyrifera_M_contig99148:2..496- (Macrocystis pyrifera P11B4 male)
AGGCACCACAGGCGGTGCCTGGTGGAGGAGCTGGGTGATGGCAGCAGCGA
GCTAGAGTTCACTGAGCACATCCTCGAGATAGACGACGACTCGAAGAACT
ACCATGCTTGGTCCCACCGGCAGTGGGCGCTGAAGACGTACGGCCTCTGG
GAGAAGGAGCTGCAATTCGTCGAGACGAAACTGCAGGAGGACCTGAGGAA
TAACTCTGCTTGGAACCACCGGTTTTTTGTGGTCTCCAACACAGAGGACC
TCTCGAATGCTGCGGTGCGAAAGCGAGAGATCGATTACGCCCTCTCCTGG
ATCAGGAGGGCGCCCAACAACCAAAGCCCATGGAATTACATCTACGGCTT
TGCAAACGGCCGGATATCACAGGCAGAGGGCCTCCAGGAGCAGTGCCGCG
ACTGTGTCGAGAAATACCCCACCTCTTCGCACGCCCTCGCGATGCTCTGC
GACATCCTCGACGACAAGGGCACCGCCGAGTCTCTTGCCGAAGCCAGGCA
CCACAGGCGGTGCCTGGTGGAGGAGCTGGGTGATGGCAGCAGCGAGCTAG
AGTTCACTGAGCACATCCTCGAGATAGACGACGACTCGAAGAACTACCAT
GCTTGGTCCCACCGGCAGTGGGCGCTGAAGACGTACGGCCTCTGGGAGAA
GGAGCTGCAATTCGTCGAGACGAAACTGCAGGAGGACCTGAGGAATAACT
CTGCTTGGAACCACCGGTTTTTTGTGGTCTCCAACACAGAGGACCTCTCG
AATGCTGCGGTGCGAAAGCGAGAGATCGATTACGCCCTCTCCTGGATCAG
GAGGGCGCCCAACAACCAAAGCCCATGGAATTACATCTACGGCTTTGCAA
ACGGCCGGATATCACAGGCAGAGGGCCTCCAGGAGCAGTGCCGCGACTGT
GTCGAGAAATACCCCACCTCTTCGCACGCCCTCGCGATGCTCTGCGACAT
CCTCGACGACAAGGGCACCGCCGAGTCTCTTGCCGAAGCC
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