mRNA_M-pyrifera_M_contig110393.2175.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig110393.2175.1
Unique NamemRNA_M-pyrifera_M_contig110393.2175.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A5A8E375_CAFRO (AAA domain-containing protein n=3 Tax=Sar TaxID=2698737 RepID=A0A5A8E375_CAFRO)

HSP 1 Score: 203 bits (517), Expect = 1.810e-62
Identity = 100/122 (81.97%), Postives = 118/122 (96.72%), Query Frame = 1 
Query:    4 QLSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            +L+D+VGNEP+VERLK I + GNMNN+ILAGPPGTGKTTS++CLARA+LGPA +K+AVLELNASDDRGIDVVRNKIK+FAQKKVTLPPGRHKI+ILDEADSMT++AQQ+LRRTME++SSTTR
Sbjct:   56 ELADIVGNEPIVERLKIIGEEGNMNNMILAGPPGTGKTTSVLCLARALLGPA-MKDAVLELNASDDRGIDVVRNKIKVFAQKKVTLPPGRHKIIILDEADSMTASAQQALRRTMEVFSSTTR 176
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A5D6XUN5_9STRA (AAA domain-containing protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XUN5_9STRA)

HSP 1 Score: 189 bits (481), Expect = 1.490e-57
Identity = 98/121 (80.99%), Postives = 111/121 (91.74%), Query Frame = 1 
Query:    7 LSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            LSDVVGN   +E L+A+A  GNM N+I++GPPGTGKTTSI+CLAR +LGPAV K+AVLELNASDDRGID VRNKIKMFAQ+KVTLPPGRHKIVILDEADSMT+AAQQ+LRRTMEI+SSTTR
Sbjct:   18 LSDVVGNADTIECLRAMAKDGNMTNLIISGPPGTGKTTSILCLARELLGPAV-KQAVLELNASDDRGIDTVRNKIKMFAQQKVTLPPGRHKIVILDEADSMTTAAQQALRRTMEIFSSTTR 137
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: F2U8I7_SALR5 (Replication factor C subunit 2 n=1 Tax=Salpingoeca rosetta (strain ATCC 50818 / BSB-021) TaxID=946362 RepID=F2U8I7_SALR5)

HSP 1 Score: 189 bits (481), Expect = 1.530e-57
Identity = 94/121 (77.69%), Postives = 110/121 (90.91%), Query Frame = 1 
Query:    7 LSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            L D+VGNE  V+RLK IA+AGNM N+I+AGPPGTGKTTSI+CLARA+LG  V KEAV+E NASDDRGI+VVRN IKMF++KKVTLPPGRHKIVILDE DSMTSAAQQ+LRRTME++++TTR
Sbjct:   14 LKDIVGNEETVDRLKVIAEAGNMPNLIIAGPPGTGKTTSILCLARALLGEDVYKEAVMETNASDDRGIEVVRNNIKMFSRKKVTLPPGRHKIVILDEVDSMTSAAQQALRRTMEMYANTTR 134
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A6F8ZZN9_9TELE (AAA domain-containing protein n=1 Tax=Coregonus sp. 'balchen' TaxID=861768 RepID=A0A6F8ZZN9_9TELE)

HSP 1 Score: 184 bits (466), Expect = 4.240e-57
Identity = 94/123 (76.42%), Postives = 110/123 (89.43%), Query Frame = 1 
Query:    1 LQLSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            L+L+++VGNE  V RL+  A  GN+ NII+AGPPGTGKTTSI+CLARA+LGPA +K+AVLELNAS+DRGIDVVRNKIKMFAQ+KVTLP GRHKI+ILDEADSMT  AQQ+LRRTMEI+S TTR
Sbjct:   42 LKLNEIVGNEETVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPA-MKDAVLELNASNDRGIDVVRNKIKMFAQQKVTLPKGRHKIIILDEADSMTDGAQQALRRTMEIYSKTTR 163
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A2D4C0G1_PYTIN (Replication factor C subunit 2 n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4C0G1_PYTIN)

HSP 1 Score: 188 bits (478), Expect = 4.580e-57
Identity = 97/121 (80.17%), Postives = 111/121 (91.74%), Query Frame = 1 
Query:    7 LSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            LSDVVGN   ++ L+A+A  GNM N+I+AGPPGTGKTTSI+CLAR +LGPA +K+AVLELNASDDRGID VRNKIKMFAQ+KVTLPPGRHKIVILDEADSMT+AAQQ+LRRTMEI+SSTTR
Sbjct:   18 LSDVVGNAETIDCLRAMARDGNMTNLIIAGPPGTGKTTSILCLARELLGPA-MKQAVLELNASDDRGIDTVRNKIKMFAQQKVTLPPGRHKIVILDEADSMTAAAQQALRRTMEIFSSTTR 137
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A0G4G9B3_9ALVE (AAA domain-containing protein (Fragment) n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4G9B3_9ALVE)

HSP 1 Score: 183 bits (465), Expect = 5.140e-57
Identity = 95/122 (77.87%), Postives = 111/122 (90.98%), Query Frame = 1 
Query:    4 QLSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            +L+DVVGN+  V+RLKAIA+ GN+ NII++GPPGTGKTTSI+CLA  +LG +  K AVLELNASDDRGIDVVRNKIKMFAQ+KV+LPPGRHKIVILDEADSMT AAQQ++RRTMEI+SSTTR
Sbjct:   12 KLADVVGNDLTVDRLKAIAEDGNLPNIIISGPPGTGKTTSILCLATELLG-SYRKNAVLELNASDDRGIDVVRNKIKMFAQQKVSLPPGRHKIVILDEADSMTEAAQQAMRRTMEIYSSTTR 132
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A7K8X6B8_9PICI (RFC2 factor (Fragment) n=1 Tax=Eubucco bourcierii TaxID=91767 RepID=A0A7K8X6B8_9PICI)

HSP 1 Score: 183 bits (465), Expect = 5.650e-57
Identity = 94/123 (76.42%), Postives = 109/123 (88.62%), Query Frame = 1 
Query:    1 LQLSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            ++L ++VGNE  V RL+  A  GN+ NII+AGPPGTGKTTSI+CLARA+LGPA LK+AVLELNAS+DRGIDVVRNKIKMFAQ+KVTLP GRHKI+ILDEADSMT  AQQ+LRRTMEI+S TTR
Sbjct:   47 MKLCEIVGNEDTVSRLEVFAKEGNVPNIIIAGPPGTGKTTSILCLARALLGPA-LKDAVLELNASNDRGIDVVRNKIKMFAQQKVTLPKGRHKIIILDEADSMTDGAQQALRRTMEIYSKTTR 168
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A7S0WJW7_9CHLO (Hypothetical protein n=1 Tax=Chlamydomonas leiostraca TaxID=1034604 RepID=A0A7S0WJW7_9CHLO)

HSP 1 Score: 187 bits (476), Expect = 1.140e-56
Identity = 96/121 (79.34%), Postives = 108/121 (89.26%), Query Frame = 1 
Query:    7 LSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            + D+VGN+  V RL+ IA+ GNM N+ILAGPPGTGKTTSI+CLA  +LGP   KEAVLELNASDDRGIDVVRNKIKMF+QKKVTLPPGRHK+VILDEADSMTS AQQ+LRRTMEI+SSTTR
Sbjct:   32 IKDIVGNDEAVSRLQVIAEEGNMPNLILAGPPGTGKTTSILCLASQLLGPNY-KEAVLELNASDDRGIDVVRNKIKMFSQKKVTLPPGRHKVVILDEADSMTSGAQQALRRTMEIYSSTTR 151
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: A0A7S4KKA6_9EUKA (Hypothetical protein n=2 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4KKA6_9EUKA)

HSP 1 Score: 187 bits (474), Expect = 1.220e-56
Identity = 95/121 (78.51%), Postives = 104/121 (85.95%), Query Frame = 1 
Query:    7 LSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            + DVVGNE  V RL+ IA  GNM NII+AGPPGTGKTTSI+CLAR +LGP   KE VLELNASDDRGI +VR KIKMFAQKKVTLPPGRHKIVILDEAD +TSAAQQ+LRRTMEI+S TTR
Sbjct:    1 MEDVVGNEETVSRLRVIAKEGNMPNIIIAGPPGTGKTTSILCLARQLLGPEKAKEGVLELNASDDRGISIVREKIKMFAQKKVTLPPGRHKIVILDEADQITSAAQQALRRTMEIYSDTTR 121
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BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Match: UPI001FB0F011 (replication factor C subunit 2 isoform X2 n=1 Tax=Homo sapiens TaxID=9606 RepID=UPI001FB0F011)

HSP 1 Score: 182 bits (462), Expect = 2.430e-56
Identity = 93/123 (75.61%), Postives = 110/123 (89.43%), Query Frame = 1 
Query:    1 LQLSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAMLGPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEADSMTSAAQQSLRRTMEIWSSTTR 369
            ++L+++VGNE  V RL+  A  GN+ NII+AGPPGTGKTTSI+CLARA+LGPA LK+A+LELNAS+DRGIDVVRNKIKMFAQ+KVTLP GRHKI+ILDEADSMT  AQQ+LRRTMEI+S TTR
Sbjct:   45 VKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPA-LKDAMLELNASNDRGIDVVRNKIKMFAQQKVTLPKGRHKIIILDEADSMTDGAQQALRRTMEIYSKTTR 166
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig110393.2175.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8E375_CAFRO1.810e-6281.97AAA domain-containing protein n=3 Tax=Sar TaxID=26... [more]
A0A5D6XUN5_9STRA1.490e-5780.99AAA domain-containing protein n=1 Tax=Pythium bras... [more]
F2U8I7_SALR51.530e-5777.69Replication factor C subunit 2 n=1 Tax=Salpingoeca... [more]
A0A6F8ZZN9_9TELE4.240e-5776.42AAA domain-containing protein n=1 Tax=Coregonus sp... [more]
A0A2D4C0G1_PYTIN4.580e-5780.17Replication factor C subunit 2 n=1 Tax=Pythium ins... [more]
A0A0G4G9B3_9ALVE5.140e-5777.87AAA domain-containing protein (Fragment) n=1 Tax=C... [more]
A0A7K8X6B8_9PICI5.650e-5776.42RFC2 factor (Fragment) n=1 Tax=Eubucco bourcierii ... [more]
A0A7S0WJW7_9CHLO1.140e-5679.34Hypothetical protein n=1 Tax=Chlamydomonas leiostr... [more]
A0A7S4KKA6_9EUKA1.220e-5678.51Hypothetical protein n=2 Tax=Paramoeba aestuarina ... [more]
UPI001FB0F0112.430e-5675.61replication factor C subunit 2 isoform X2 n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig110393contigM-pyrifera_M_contig110393:50..598 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeOpisthokonta
Seed ortholog score190.7
Seed ortholog evalue4.2e-46
Seed eggNOG ortholog946362.XP_004994518.1
Preferred nameRFC2
KEGG koko:K10755
KEGG Pathwayko03030,ko03420,ko03430,map03030,map03420,map03430
KEGG ModuleM00289,M00295
Hectar predicted targeting categoryother localisation
GOsGO:0000075,GO:0000076,GO:0000077,GO:0000166,GO:0000228,GO:0000278,GO:0000723,GO:0000724,GO:0000725,GO:0000731,GO:0000785,GO:0000790,GO:0000819,GO:0003674,GO:0003676,GO:0003677,GO:0003824,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005657,GO:0005663,GO:0005694,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006271,GO:0006272,GO:0006275,GO:0006281,GO:0006283,GO:0006289,GO:0006296,GO:0006297,GO:0006298,GO:0006301,GO:0006302,GO:0006310,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0007049,GO:0007059,GO:0007062,GO:0008144,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009314,GO:0009411,GO:0009416,GO:0009628,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010556,GO:0010557,GO:0010604,GO:0016043,GO:0016070,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017076,GO:0017111,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0019725,GO:0019899,GO:0019985,GO:0022402,GO:0022616,GO:0030554,GO:0030894,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031389,GO:0031390,GO:0031391,GO:0031570,GO:0031974,GO:0031981,GO:0032200,GO:0032201,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0032993,GO:0033260,GO:0033554,GO:0033683,GO:0034641,GO:0034644,GO:0034645,GO:0034654,GO:0035639,GO:0036094,GO:0042276,GO:0042592,GO:0042623,GO:0042769,GO:0043085,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043596,GO:0043599,GO:0043601,GO:0044093,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044454,GO:0044464,GO:0044786,GO:0045786,GO:0045935,GO:0046483,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051052,GO:0051054,GO:0051171,GO:0051173,GO:0051276,GO:0051338,GO:0051347,GO:0051606,GO:0051716,GO:0051726,GO:0060249,GO:0060255,GO:0061860,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070914,GO:0070987,GO:0071214,GO:0071478,GO:0071482,GO:0071704,GO:0071840,GO:0071897,GO:0080090,GO:0090304,GO:0090305,GO:0090329,GO:0090618,GO:0097159,GO:0097367,GO:0098813,GO:0104004,GO:1900262,GO:1900264,GO:1901265,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1902296,GO:1902319,GO:1902494,GO:1902969,GO:1902983,GO:1903047,GO:1903460,GO:1904949,GO:2000112,GO:2000278,GO:2000573
EggNOG free text desc.positive regulation of DNA-directed DNA polymerase activity
EggNOG OGs38G2T@33154,COG0470@1,KOG0991@2759
COG Functional cat.L
Best tax levelOpisthokonta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03032,ko03036,ko03400
Exons2
Model size369
Cds size369
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815615.0521746-CDS-M-pyrifera_M_contig110393:49..1421622815615.0521746-CDS-M-pyrifera_M_contig110393:49..142Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110393 50..142 +
1692277488.44818-CDS-M-pyrifera_M_contig110393:49..1421692277488.44818-CDS-M-pyrifera_M_contig110393:49..142Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110393 50..142 +
1622815615.0640194-CDS-M-pyrifera_M_contig110393:322..5981622815615.0640194-CDS-M-pyrifera_M_contig110393:322..598Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110393 323..598 +
1692277488.4595854-CDS-M-pyrifera_M_contig110393:322..5981692277488.4595854-CDS-M-pyrifera_M_contig110393:322..598Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110393 323..598 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig110393.2175.1prot_M-pyrifera_M_contig110393.2175.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig110393 50..598 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig110393.2175.1

>prot_M-pyrifera_M_contig110393.2175.1 ID=prot_M-pyrifera_M_contig110393.2175.1|Name=mRNA_M-pyrifera_M_contig110393.2175.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=123bp
LQLSDVVGNEPVVERLKAIADAGNMNNIILAGPPGTGKTTSIMCLARAML
GPAVLKEAVLELNASDDRGIDVVRNKIKMFAQKKVTLPPGRHKIVILDEA
DSMTSAAQQSLRRTMEIWSSTTR
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mRNA from alignment at M-pyrifera_M_contig110393:50..598+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig110393.2175.1 ID=mRNA_M-pyrifera_M_contig110393.2175.1|Name=mRNA_M-pyrifera_M_contig110393.2175.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=549bp|location=Sequence derived from alignment at M-pyrifera_M_contig110393:50..598+ (Macrocystis pyrifera P11B4 male)
CTGCAGTTGTCCGATGTGGTGGGAAATGAACCCGTGGTCGAGCGTCTCAA
AGCCATTGCGGACGCTGGAAACATGAACAACATTATTTTGGCTGTATGTC
GGAATGGTTGGGGAAGGTTTGGGTGGGAAATGGGGTGATCAAGGGTATAT
AGTGATTGTGATGTGGGTTTTATAGGGTAGATAGGAAGGGGAAAGGTGAG
AAAATGGAGGTTGAAAGGATTTGGGGGTAGAAACTGAGGTATTGGGGCTT
GTATGATTTATGTTGGATTGCAGGGACCACCAGGGACAGGGAAAACGACG
TCAATTATGTGTCTTGCACGTGCTATGCTTGGACCAGCAGTGCTAAAGGA
AGCAGTGTTGGAATTGAATGCTTCAGACGATCGGGGGATTGATGTGGTGC
GAAACAAGATCAAAATGTTTGCGCAGAAGAAGGTGACATTGCCTCCAGGG
CGACACAAAATTGTGATCTTGGATGAGGCTGATAGTATGACGAGTGCAGC
ACAACAAAGTCTTCGTCGCACAATGGAGATCTGGAGCTCCACAACTCGG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig110393:50..598+

>mRNA_M-pyrifera_M_contig110393.2175.1 ID=mRNA_M-pyrifera_M_contig110393.2175.1|Name=mRNA_M-pyrifera_M_contig110393.2175.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=738bp|location=Sequence derived from alignment at M-pyrifera_M_contig110393:50..598+ (Macrocystis pyrifera P11B4 male)
CTGCAGTTGTCCGATGTGGTGGGAAATGAACCCGTGGTCGAGCGTCTCAA
AGCCATTGCGGACGCTGGAAACATGAACAACATTATTTTGGCTCTGCAGT
TGTCCGATGTGGTGGGAAATGAACCCGTGGTCGAGCGTCTCAAAGCCATT
GCGGACGCTGGAAACATGAACAACATTATTTTGGCTGGACCACCAGGGAC
AGGGAAAACGACGTCAATTATGTGTCTTGCACGTGCTATGCTTGGACCAG
CAGTGCTAAAGGAAGCAGTGTTGGAATTGAATGCTTCAGACGATCGGGGG
ATTGATGTGGTGCGAAACAAGATCAAAATGTTTGCGCAGAAGAAGGTGAC
ATTGCCTCCAGGGCGACACAAAATTGTGATCTTGGATGAGGCTGATAGTA
TGACGAGTGCAGCACAACAAAGTCTTCGTCGCACAATGGAGATCTGGAGC
TCCACAACTCGGGGACCACCAGGGACAGGGAAAACGACGTCAATTATGTG
TCTTGCACGTGCTATGCTTGGACCAGCAGTGCTAAAGGAAGCAGTGTTGG
AATTGAATGCTTCAGACGATCGGGGGATTGATGTGGTGCGAAACAAGATC
AAAATGTTTGCGCAGAAGAAGGTGACATTGCCTCCAGGGCGACACAAAAT
TGTGATCTTGGATGAGGCTGATAGTATGACGAGTGCAGCACAACAAAGTC
TTCGTCGCACAATGGAGATCTGGAGCTCCACAACTCGG
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